AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02897


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02897.t1 MTR_2g027100 97.590 166 4 0 1 166 1 166 4.65e-116 325
MS.gene02897.t1 MTR_8g088320 46.012 163 75 2 1 157 161 316 7.02e-40 137
MS.gene02897.t1 MTR_2g011590 43.791 153 84 1 5 157 4 154 8.29e-40 132
MS.gene02897.t1 MTR_3g067585 43.312 157 87 1 1 157 1 155 1.68e-39 132
MS.gene02897.t1 MTR_6g069470 40.127 157 92 2 1 157 13 167 5.77e-37 125
MS.gene02897.t1 MTR_2g030230 44.586 157 86 1 1 157 168 323 1.31e-36 129
MS.gene02897.t1 MTR_8g107340 42.675 157 88 1 1 157 17 171 2.44e-36 124
MS.gene02897.t1 MTR_3g017500 45.223 157 85 1 1 157 209 364 1.58e-33 122
MS.gene02897.t1 MTR_3g017500 45.223 157 85 1 1 157 239 394 3.36e-33 121
MS.gene02897.t1 MTR_8g038400 42.675 157 89 1 1 157 206 361 5.01e-31 115
MS.gene02897.t1 MTR_4g084280 39.474 152 90 1 1 152 1 150 6.92e-31 110
MS.gene02897.t1 MTR_1g031650 42.282 149 83 2 5 152 6 152 5.51e-30 107
MS.gene02897.t1 MTR_1g031650 40.517 116 67 1 37 152 2 115 2.15e-20 82.0
MS.gene02897.t1 MTR_1g008950 35.417 96 60 2 6 99 4 99 9.60e-13 62.0
MS.gene02897.t1 MTR_8g032320 37.500 96 58 2 6 99 4 99 1.40e-12 62.0
MS.gene02897.t1 MTR_8g032300 37.363 91 55 2 6 94 4 94 1.10e-11 59.7
MS.gene02897.t1 MTR_6g084400 38.053 113 61 5 7 113 484 593 1.27e-11 62.4
MS.gene02897.t1 MTR_5g079880 36.364 99 57 4 5 97 3 101 2.76e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02897.t1 AT5G47710 69.277 166 51 0 1 166 1 166 1.55e-84 246
MS.gene02897.t1 AT5G47710 69.277 166 51 0 1 166 1 166 1.55e-84 246
MS.gene02897.t1 AT5G47710 69.277 166 51 0 1 166 1 166 1.55e-84 246
MS.gene02897.t1 AT5G47710 69.277 166 51 0 1 166 1 166 1.55e-84 246
MS.gene02897.t1 AT1G73580 45.223 157 84 1 1 157 4 158 4.00e-40 133
MS.gene02897.t1 AT1G70790 45.342 161 85 3 1 159 1 160 4.88e-40 134
MS.gene02897.t1 AT1G70790 45.342 161 85 3 1 159 1 160 4.88e-40 134
MS.gene02897.t1 AT1G70790 45.342 161 85 3 1 159 1 160 4.88e-40 134
MS.gene02897.t1 AT2G01540 44.156 154 84 2 5 157 6 158 4.87e-38 128
MS.gene02897.t1 AT3G17980 42.038 157 89 2 1 157 13 167 1.03e-37 127
MS.gene02897.t1 AT5G37740 40.506 158 93 1 1 157 1 158 2.36e-37 126
MS.gene02897.t1 AT1G66360 38.854 157 96 0 1 157 1 157 1.57e-36 124
MS.gene02897.t1 AT4G05330 43.949 157 87 1 1 157 140 295 2.21e-36 127
MS.gene02897.t1 AT4G05330 43.949 157 87 1 1 157 175 330 7.21e-36 127
MS.gene02897.t1 AT4G05330 43.949 157 87 1 1 157 175 330 7.21e-36 127
MS.gene02897.t1 AT4G21160 42.675 157 89 1 1 157 176 331 1.01e-35 127
MS.gene02897.t1 AT4G21160 42.675 157 89 1 1 157 176 331 1.01e-35 127
MS.gene02897.t1 AT4G21160 42.675 157 89 1 1 157 176 331 1.01e-35 127
MS.gene02897.t1 AT4G21160 42.675 157 89 1 1 157 176 331 1.01e-35 127
MS.gene02897.t1 AT1G70810 40.764 157 91 2 1 157 1 155 1.19e-35 122
MS.gene02897.t1 AT1G23140 37.576 165 101 2 1 165 1 163 1.54e-34 119
MS.gene02897.t1 AT1G48590 39.490 157 93 2 1 157 6 160 2.72e-34 119
MS.gene02897.t1 AT1G48590 39.490 157 93 2 1 157 6 160 2.72e-34 119
MS.gene02897.t1 AT1G48590 39.490 157 93 2 1 157 37 191 4.93e-34 119
MS.gene02897.t1 AT5G37740 38.095 168 93 2 1 157 1 168 6.98e-34 118
MS.gene02897.t1 AT3G07940 44.586 157 86 1 1 157 224 379 1.50e-33 122
MS.gene02897.t1 AT1G70800 36.306 157 98 1 1 157 10 164 2.92e-31 111
MS.gene02897.t1 AT1G70790 42.149 121 68 2 40 159 4 123 7.03e-27 99.4
MS.gene02897.t1 AT1G48590 32.484 157 72 3 1 157 37 159 1.58e-19 80.9
MS.gene02897.t1 AT1G63220 38.889 90 53 2 6 93 4 93 8.31e-11 57.4

Find 35 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACCTTTAGTTGCCCTAAT+TGG 0.297676 2.4:+61830518 None:intergenic
CTTCATTCCAAACAGGATTC+AGG 0.299774 2.4:+61829896 None:intergenic
ACCCTCGGGAATTCTCAATT+TGG 0.308994 2.4:-61829851 MS.gene02897:intron
CCTTATGTTGTTCTCAAACT+AGG 0.330352 2.4:-61830445 MS.gene02897:CDS
AGCATAATGAGTGACCAATT+AGG 0.335455 2.4:-61830532 None:intergenic
GCATAATGAGTGACCAATTA+GGG 0.366299 2.4:-61830531 None:intergenic
CTTCCGGTGAGCAAACATTA+AGG 0.379148 2.4:-61829367 MS.gene02897:CDS
TGTTCTCAAACTAGGGAATC+AGG 0.396033 2.4:-61830437 MS.gene02897:intron
ATAGCTGCCTGAATCCTGTT+TGG 0.398410 2.4:-61829903 MS.gene02897:CDS
GCACGGTTGTGCAGAATGTT+TGG 0.399741 2.4:-61829283 MS.gene02897:CDS
AGCAGTATCAATTGTGTTAA+CGG 0.411045 2.4:-61829305 MS.gene02897:CDS
TTCAGCTCTTCATTCCAAAC+AGG 0.413548 2.4:+61829889 None:intergenic
TTGTGCAGAATGTTTGGTTA+AGG 0.441247 2.4:-61829277 MS.gene02897:CDS
AATCTAGCTGCAGAGACTAA+TGG 0.452322 2.4:+61829410 None:intergenic
CTTATGTTGTTCTCAAACTA+GGG 0.459241 2.4:-61830444 MS.gene02897:CDS
CCTAGTTTGAGAACAACATA+AGG 0.471060 2.4:+61830445 None:intergenic
CTGCAGAGACTAATGGTTGA+AGG 0.477932 2.4:+61829417 None:intergenic
GAGTGTCAATTCTATCTCTC+CGG 0.491542 2.4:+61829238 None:intergenic
TTTGAAAGCTGATGACAAGA+TGG 0.495191 2.4:-61829456 MS.gene02897:CDS
GATCGCTGGTCTTGAAATCT+CGG 0.506401 2.4:+61830467 None:intergenic
TTGAAAGCTGATGACAAGAT+GGG 0.520635 2.4:-61829455 MS.gene02897:CDS
TCATAGTTGTGCAAGGGAAA+AGG 0.522477 2.4:-61830498 MS.gene02897:CDS
CTTCCTTAATGTTTGCTCAC+CGG 0.528914 2.4:+61829364 None:intergenic
TTTCACTTTGACAGAACCCT+CGG 0.539049 2.4:-61829866 MS.gene02897:CDS
AGGCTTCGCGAAGTAGAATC+CGG 0.566418 2.4:-61829257 MS.gene02897:CDS
GAGAACAACATAAGGATCGC+TGG 0.570782 2.4:+61830453 None:intergenic
TGACCAATTAGGGCAACTAA+AGG 0.571901 2.4:-61830521 MS.gene02897:CDS
CTAAAGGTCATAGTTGTGCA+AGG 0.584222 2.4:-61830505 MS.gene02897:CDS
CGGTGAGCAAACATTAAGGA+AGG 0.588349 2.4:-61829363 MS.gene02897:CDS
TTCACTTTGACAGAACCCTC+GGG 0.609431 2.4:-61829865 MS.gene02897:CDS
TAAAGGTCATAGTTGTGCAA+GGG 0.611606 2.4:-61830504 MS.gene02897:CDS
CACCAAATTGAGAATTCCCG+AGG 0.612418 2.4:+61829849 None:intergenic
TATCAATTGTGTTAACGGCA+CGG 0.623235 2.4:-61829300 MS.gene02897:CDS
AGTTGTGCAAGGGAAAAGGT+TGG 0.639430 2.4:-61830494 MS.gene02897:CDS
ACCAAATTGAGAATTCCCGA+GGG 0.673124 2.4:+61829850 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACTACTAAATAATGTTAA+GGG + chr2.4:61829739-61829758 None:intergenic 15.0%
!! TATACTACTAAATAATGTTA+AGG + chr2.4:61829740-61829759 None:intergenic 15.0%
! ATGCATCTTCAAAGTTTATT+TGG + chr2.4:61829702-61829721 None:intergenic 25.0%
! CTTATTTGAGCTTATCTAAT+AGG - chr2.4:61830030-61830049 MS.gene02897:intron 25.0%
! GTTTCATATTCATCTTTAGT+TGG - chr2.4:61829332-61829351 MS.gene02897:CDS 25.0%
! TATGTTATATGCGCTTAATT+AGG - chr2.4:61830179-61830198 MS.gene02897:intron 25.0%
! TTTGACATTAAGGACATATT+TGG - chr2.4:61829504-61829523 MS.gene02897:intron 25.0%
!! AATTTCTGAAAAACACTTGT+GGG - chr2.4:61829610-61829629 MS.gene02897:intron 25.0%
!! CTATTTTCTTAAGCTATTAC+CGG + chr2.4:61829405-61829424 None:intergenic 25.0%
!! TAATTTCTGAAAAACACTTG+TGG - chr2.4:61829609-61829628 MS.gene02897:intron 25.0%
!!! ATTAGGTTTTTTATCTGAAG+AGG - chr2.4:61830196-61830215 MS.gene02897:intron 25.0%
!!! CTAAACTGTTTTTGACATTA+AGG - chr2.4:61829494-61829513 MS.gene02897:intron 25.0%
!!! TTAGGTTTTTTATCTGAAGA+GGG - chr2.4:61830197-61830216 MS.gene02897:intron 25.0%
AAAATCAGACATATAGACAC+CGG - chr2.4:61829383-61829402 MS.gene02897:CDS 30.0%
AGCAGTATCAATTGTGTTAA+CGG - chr2.4:61830426-61830445 MS.gene02897:intron 30.0%
CTTATGTTGTTCTCAAACTA+GGG - chr2.4:61829287-61829306 MS.gene02897:CDS 30.0%
GTATATAGGACTGTTTAGAA+TGG - chr2.4:61830007-61830026 MS.gene02897:intron 30.0%
TGCAACTCAGTTATAAATGA+AGG + chr2.4:61830223-61830242 None:intergenic 30.0%
TTCTATAGCAAAAGCTATCA+TGG + chr2.4:61830124-61830143 None:intergenic 30.0%
! ATTTTGCGTTAGTGTTTCAT+AGG + chr2.4:61829368-61829387 None:intergenic 30.0%
! CTGAGTTGCATTTTGAATAT+AGG - chr2.4:61830233-61830252 MS.gene02897:intron 30.0%
! GAGTTTACTCATATTTTGCT+TGG - chr2.4:61829904-61829923 MS.gene02897:CDS 30.0%
! GATTATCGAATGAGCTATTT+TGG - chr2.4:61829546-61829565 MS.gene02897:intron 30.0%
! TACAAGACAATTTTCAGAGT+CGG + chr2.4:61830400-61830419 None:intergenic 30.0%
! TTACTCATATTTTGCTTGGA+AGG - chr2.4:61829908-61829927 MS.gene02897:CDS 30.0%
!! TTATGATCAGAAGCTGTTTT+TGG - chr2.4:61829582-61829601 MS.gene02897:intron 30.0%
AAGATGCATGCTCTTTCTAA+TGG - chr2.4:61829713-61829732 MS.gene02897:intron 35.0%
CCTAGTTTGAGAACAACATA+AGG + chr2.4:61829289-61829308 None:intergenic 35.0%
CCTTATGTTGTTCTCAAACT+AGG - chr2.4:61829286-61829305 MS.gene02897:CDS 35.0%
GTACTCTACATGCTGTATAT+AGG - chr2.4:61829993-61830012 MS.gene02897:intron 35.0%
TAAAGGTCATAGTTGTGCAA+GGG - chr2.4:61829227-61829246 MS.gene02897:CDS 35.0%
TATCAATTGTGTTAACGGCA+CGG - chr2.4:61830431-61830450 MS.gene02897:intron 35.0%
TTGAAAGCTGATGACAAGAT+GGG - chr2.4:61830276-61830295 MS.gene02897:intron 35.0%
TTGAATGTAACCACATGTGT+CGG - chr2.4:61829431-61829450 MS.gene02897:CDS 35.0%
TTGAGCTTATCTAATAGGCT+TGG - chr2.4:61830035-61830054 MS.gene02897:intron 35.0%
TTGTGCAGAATGTTTGGTTA+AGG - chr2.4:61830454-61830473 MS.gene02897:CDS 35.0%
TTTGAAAGCTGATGACAAGA+TGG - chr2.4:61830275-61830294 MS.gene02897:intron 35.0%
! ACAAGACAATTTTCAGAGTC+GGG + chr2.4:61830399-61830418 None:intergenic 35.0%
! CTAGGTGATGTATTTTACTG+TGG - chr2.4:61829935-61829954 MS.gene02897:intron 35.0%
!! GACATTTTAAGAGTCTCTTC+CGG - chr2.4:61830348-61830367 MS.gene02897:intron 35.0%
AATCTAGCTGCAGAGACTAA+TGG + chr2.4:61830324-61830343 None:intergenic 40.0%
ACCAAATTGAGAATTCCCGA+GGG + chr2.4:61829884-61829903 None:intergenic 40.0%
ACGCCTTCAATATGAAGTGT+CGG - chr2.4:61829469-61829488 MS.gene02897:CDS 40.0%
CAATGCGCTATATATTAGCC+TGG - chr2.4:61829967-61829986 MS.gene02897:intron 40.0%
CTAAAGGTCATAGTTGTGCA+AGG - chr2.4:61829226-61829245 MS.gene02897:CDS 40.0%
CTTCATTCCAAACAGGATTC+AGG + chr2.4:61829838-61829857 None:intergenic 40.0%
CTTCCTTAATGTTTGCTCAC+CGG + chr2.4:61830370-61830389 None:intergenic 40.0%
GAGTGTCAATTCTATCTCTC+CGG + chr2.4:61830496-61830515 None:intergenic 40.0%
TCATAGTTGTGCAAGGGAAA+AGG - chr2.4:61829233-61829252 MS.gene02897:CDS 40.0%
TGACCAATTAGGGCAACTAA+AGG - chr2.4:61829210-61829229 MS.gene02897:CDS 40.0%
TGACCTTTAGTTGCCCTAAT+TGG + chr2.4:61829216-61829235 None:intergenic 40.0%
TGTTCTCAAACTAGGGAATC+AGG - chr2.4:61829294-61829313 MS.gene02897:CDS 40.0%
TTCAGCTCTTCATTCCAAAC+AGG + chr2.4:61829845-61829864 None:intergenic 40.0%
TTTCACTTTGACAGAACCCT+CGG - chr2.4:61829865-61829884 MS.gene02897:CDS 40.0%
! GTACCGACACTTCATATTGA+AGG + chr2.4:61829475-61829494 None:intergenic 40.0%
!! TCGAATGAGCTATTTTGGAG+AGG - chr2.4:61829551-61829570 MS.gene02897:intron 40.0%
!!! TTTTGAAGTAGCAACAGGTG+TGG + chr2.4:61830526-61830545 None:intergenic 40.0%
!!! TTTTGCTTGGAAGGTACTCT+AGG - chr2.4:61829917-61829936 MS.gene02897:CDS 40.0%
ACCCTCGGGAATTCTCAATT+TGG - chr2.4:61829880-61829899 MS.gene02897:CDS 45.0%
AGTTGTGCAAGGGAAAAGGT+TGG - chr2.4:61829237-61829256 MS.gene02897:CDS 45.0%
ATAGCTGCCTGAATCCTGTT+TGG - chr2.4:61829828-61829847 MS.gene02897:intron 45.0%
CACCAAATTGAGAATTCCCG+AGG + chr2.4:61829885-61829904 None:intergenic 45.0%
CGGTGAGCAAACATTAAGGA+AGG - chr2.4:61830368-61830387 MS.gene02897:intron 45.0%
CTGCAGAGACTAATGGTTGA+AGG + chr2.4:61830317-61830336 None:intergenic 45.0%
CTTCCGGTGAGCAAACATTA+AGG - chr2.4:61830364-61830383 MS.gene02897:intron 45.0%
GAGAACAACATAAGGATCGC+TGG + chr2.4:61829281-61829300 None:intergenic 45.0%
TTCACTTTGACAGAACCCTC+GGG - chr2.4:61829866-61829885 MS.gene02897:CDS 45.0%
! CTTCATATTGAAGGCGTGTC+TGG + chr2.4:61829466-61829485 None:intergenic 45.0%
! GCGCTATATATTAGCCTGGA+AGG - chr2.4:61829971-61829990 MS.gene02897:intron 45.0%
! GTTTTCAACACCGACACATG+TGG + chr2.4:61829444-61829463 None:intergenic 45.0%
!! GATCGCTGGTCTTGAAATCT+CGG + chr2.4:61829267-61829286 None:intergenic 45.0%
AGGCTTCGCGAAGTAGAATC+CGG - chr2.4:61830474-61830493 MS.gene02897:CDS 50.0%
GCACGGTTGTGCAGAATGTT+TGG - chr2.4:61830448-61830467 MS.gene02897:CDS 50.0%
TGACTTTCGTCTTCGCAGTC+TGG + chr2.4:61829805-61829824 None:intergenic 50.0%
! CAGCATGTAGAGTACCTTCC+AGG + chr2.4:61829988-61830007 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 61829205 61830548 61829205 ID=MS.gene02897
chr2.4 mRNA 61829205 61830548 61829205 ID=MS.gene02897.t1;Parent=MS.gene02897
chr2.4 exon 61830438 61830548 61830438 ID=MS.gene02897.t1.exon1;Parent=MS.gene02897.t1
chr2.4 CDS 61830438 61830548 61830438 ID=cds.MS.gene02897.t1;Parent=MS.gene02897.t1
chr2.4 exon 61829852 61829947 61829852 ID=MS.gene02897.t1.exon2;Parent=MS.gene02897.t1
chr2.4 CDS 61829852 61829947 61829852 ID=cds.MS.gene02897.t1;Parent=MS.gene02897.t1
chr2.4 exon 61829205 61829498 61829205 ID=MS.gene02897.t1.exon3;Parent=MS.gene02897.t1
chr2.4 CDS 61829205 61829498 61829205 ID=cds.MS.gene02897.t1;Parent=MS.gene02897.t1
Gene Sequence

>MS.gene02897

ATGAGTGACCAATTAGGGCAACTAAAGGTCATAGTTGTGCAAGGGAAAAGGTTGGTGATCCGAGATTTCAAGACCAGCGATCCTTATGTTGTTCTCAAACTAGGGAATCAGGTAACTTACAATTTATGTTTCATATTCATCTTTAGTTGGTTTTTCTAGACCTATGAAACACTAACGCAAAATCAGACATATAGACACCGGTAATAGCTTAAGAAAATAGAAGTGATTGAATGTAACCACATGTGTCGGTGTTGAAAACCAGACACGCCTTCAATATGAAGTGTCGGTACTAAACTGTTTTTGACATTAAGGACATATTTGGATAAACATTTTAAGTAATTGATTATCGAATGAGCTATTTTGGAGAGGTTAAACACTTATGATCAGAAGCTGTTTTTGGCAGCTAATTTCTGAAAAACACTTGTGGGAATGTTATGATTTTCAAATTTACAAGTGTATTAGAATATGTAAGTACTCATATCATGAGATAAAATCCAAATAAACTTTGAAGATGCATGCTCTTTCTAATGGCCCTTAACATTATTTAGTAGTATATTTTAACTTCAAAATATAAAATGATTCATTCATTAAACTGTACCAGACTGCGAAGACGAAAGTCATTAATAGCTGCCTGAATCCTGTTTGGAATGAAGAGCTGAATTTCACTTTGACAGAACCCTCGGGAATTCTCAATTTGGTGAGTTTACTCATATTTTGCTTGGAAGGTACTCTAGGTGATGTATTTTACTGTGGTTGTGAGTTCAATGCGCTATATATTAGCCTGGAAGGTACTCTACATGCTGTATATAGGACTGTTTAGAATGGCTTATTTGAGCTTATCTAATAGGCTTGGAAGAGTTTATGAAAATGACACGACATATTCATAAGCTGTTTTCAATTTATTTTCACAAGCTTTCCATGATAGCTTTTGCTATAGAAATAACTTATACATAAGCACTTATATGATAAACTTTTATGTTATATGCGCTTAATTAGGTTTTTTATCTGAAGAGGGCCTTCATTTATAACTGAGTTGCATTTTGAATATAGGAAGTATTCGATAAAGATCTTTTGAAAGCTGATGACAAGATGGGAAATGCATTTATCAACCTTCAACCATTAGTCTCTGCAGCTAGATTAAGAGACATTTTAAGAGTCTCTTCCGGTGAGCAAACATTAAGGAAGGTTATACCCGACTCTGAAAATTGTCTTGTACGCGAAAGCAGTATCAATTGTGTTAACGGCACGGTTGTGCAGAATGTTTGGTTAAGGCTTCGCGAAGTAGAATCCGGAGAGATAGAATTGACACTCAAGTTAACCACACCTGTTGCTACTTCAAAATAG

Protein sequence

>MS.gene02897.t1

MSDQLGQLKVIVVQGKRLVIRDFKTSDPYVVLKLGNQTAKTKVINSCLNPVWNEELNFTLTEPSGILNLEVFDKDLLKADDKMGNAFINLQPLVSAARLRDILRVSSGEQTLRKVIPDSENCLVRESSINCVNGTVVQNVWLRLREVESGEIELTLKLTTPVATSK