AlfalfaGEDB | MS.gene02911

M. sativa cultivar XinJiangDaYe / MS.gene02911

KEGG: K08065
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0046982 GO:0046983 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0110165 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02911.t1	XP_013462945.1	97	166	1	1	1	162	1	166	6.20E-73	283.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02911.t1	O23310	82.6	121	13	1	1	113	1	121	4.8e-50	198.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02911.t1	I3TAX1	97.0	166	1	1	1	162	1	166	4.5e-73	283.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene02911.t1	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene42075	MS.gene02911	PPI
MS.gene87743	MS.gene02911	PPI
MS.gene77045	MS.gene02911	PPI
MS.gene87064	MS.gene02911	PPI
MS.gene48819	MS.gene02911	PPI
MS.gene76728	MS.gene02911	PPI
MS.gene02911	MS.gene82237	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02911.t1	MTR_2g026710	96.988	166	1	1	1	162	1	166	8.97e-117	327
MS.gene02911.t1	MTR_5g095740	66.061	165	39	4	1	151	1	162	5.49e-68	204
MS.gene02911.t1	MTR_3g058980	72.535	142	20	3	1	128	1	137	6.51e-66	199
MS.gene02911.t1	MTR_8g091720	74.046	131	27	2	7	130	22	152	2.25e-64	196
MS.gene02911.t1	MTR_7g061270	64.789	142	43	2	9	143	22	163	7.95e-62	188
MS.gene02911.t1	MTR_7g100650	84.694	98	15	0	9	106	22	119	3.74e-61	187
MS.gene02911.t1	MTR_1g072790	67.969	128	35	1	8	129	24	151	5.23e-61	186
MS.gene02911.t1	MTR_4g133938	72.308	130	27	3	1	121	1	130	3.54e-60	183
MS.gene02911.t1	MTR_8g093920	77.778	108	22	1	12	117	39	146	4.36e-58	180
MS.gene02911.t1	MTR_4g133952	53.947	152	54	4	2	140	48	196	9.64e-54	170
MS.gene02911.t1	MTR_0392s0020	68.269	104	33	0	4	107	9	112	3.46e-52	163
MS.gene02911.t1	MTR_1g088860	69.524	105	32	0	3	107	26	130	8.30e-52	164
MS.gene02911.t1	MTR_4g119500	61.864	118	44	1	4	120	12	129	1.05e-50	159
MS.gene02911.t1	MTR_1g039040	63.725	102	37	0	9	110	2	103	2.94e-48	154
MS.gene02911.t1	MTR_1g083070	64.130	92	33	0	13	104	4	95	8.30e-41	134
MS.gene02911.t1	MTR_1g029070	54.839	93	42	0	18	110	57	149	1.06e-30	109
MS.gene02911.t1	MTR_5g095900	44.762	105	55	1	1	102	1	105	1.52e-23	91.3
MS.gene02911.t1	MTR_1g028480	46.429	84	44	1	43	125	19	102	1.72e-19	79.0
MS.gene02911.t1	MTR_1g029100	40.789	76	45	0	18	93	7	82	1.18e-18	76.3
MS.gene02911.t1	MTR_2g056000	32.500	80	54	0	14	93	42	121	2.27e-14	67.4
MS.gene02911.t1	MTR_4g112380	32.530	83	56	0	9	91	7	89	3.07e-14	66.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02911.t1	AT4G14540	82.645	121	13	1	1	113	1	121	1.74e-69	207
MS.gene02911.t1	AT5G47640	83.929	112	16	2	12	123	26	135	4.11e-64	195
MS.gene02911.t1	AT2G38880	71.429	126	32	2	7	131	14	136	4.04e-60	183
MS.gene02911.t1	AT2G38880	71.429	126	32	2	7	131	14	136	5.13e-60	183
MS.gene02911.t1	AT2G38880	71.429	126	32	2	7	131	14	136	5.13e-60	183
MS.gene02911.t1	AT2G38880	71.429	126	32	2	7	131	14	136	5.13e-60	183
MS.gene02911.t1	AT2G38880	70.635	126	31	2	7	131	14	134	2.64e-58	178
MS.gene02911.t1	AT3G53340	62.252	151	40	4	8	143	24	172	2.94e-58	179
MS.gene02911.t1	AT3G53340	62.252	151	40	4	8	143	24	172	2.94e-58	179
MS.gene02911.t1	AT2G37060	80.808	99	19	0	8	106	25	123	3.36e-58	179
MS.gene02911.t1	AT2G37060	80.808	99	19	0	8	106	25	123	3.36e-58	179
MS.gene02911.t1	AT2G37060	80.808	99	19	0	8	106	25	123	3.36e-58	179
MS.gene02911.t1	AT2G13570	83.333	96	16	0	12	107	35	130	1.13e-57	179
MS.gene02911.t1	AT2G38880	82.653	98	16	1	7	103	14	111	8.26e-57	174
MS.gene02911.t1	AT2G38880	82.653	98	16	1	7	103	14	111	1.74e-56	174
MS.gene02911.t1	AT2G38880	82.653	98	16	1	7	103	14	111	1.74e-56	174
MS.gene02911.t1	AT2G38880	84.783	92	14	0	12	103	20	111	2.41e-56	174
MS.gene02911.t1	AT2G38880	84.783	92	14	0	12	103	20	111	2.41e-56	174
MS.gene02911.t1	AT2G38880	84.783	92	14	0	12	103	20	111	2.41e-56	174
MS.gene02911.t1	AT3G53340	75.728	103	25	0	8	110	24	126	2.61e-56	174
MS.gene02911.t1	AT3G53340	75.728	103	25	0	8	110	24	126	2.61e-56	174
MS.gene02911.t1	AT3G53340	78.125	96	21	0	8	103	24	119	1.23e-55	171
MS.gene02911.t1	AT2G47810	71.028	107	31	0	7	113	45	151	1.21e-52	165
MS.gene02911.t1	AT5G47670	66.337	101	34	0	7	107	23	123	3.25e-50	160
MS.gene02911.t1	AT5G47670	66.337	101	34	0	7	107	52	152	5.97e-50	160
MS.gene02911.t1	AT5G47670	66.337	101	34	0	7	107	52	152	5.97e-50	160
MS.gene02911.t1	AT1G21970	48.366	153	68	3	11	152	57	209	7.27e-47	152
MS.gene02911.t1	AT1G09030	61.458	96	37	0	13	108	3	98	4.02e-43	140
MS.gene02911.t1	AT5G08190	34.286	105	63	2	9	109	7	109	1.92e-17	75.1
MS.gene02911.t1	AT5G23090	32.381	105	65	2	9	109	7	109	9.03e-17	73.2
MS.gene02911.t1	AT5G08190	37.349	83	52	0	9	91	7	89	1.84e-16	72.4
MS.gene02911.t1	AT5G23090	34.940	83	54	0	9	91	7	89	7.07e-16	70.9
MS.gene02911.t1	AT5G23090	34.940	83	54	0	9	91	7	89	7.07e-16	70.9
MS.gene02911.t1	AT5G23090	34.940	83	54	0	9	91	7	89	7.07e-16	70.9
MS.gene02911.t1	AT5G23090	32.911	79	47	2	30	104	15	91	2.34e-11	58.9

Find 36 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGGGTAGTGGTGGAGTTTA+TGG	0.223086	2.4:-62033743	MS.gene02911:CDS
TCAACGGTGATGATCTTCTT+TGG	0.252720	2.4:-62033895	MS.gene02911:CDS
TTTGAGATCTTTGCGTTTGC+TGG	0.274169	2.4:+62034016	None:intergenic
ATCCTACTCACGTTTGCTAT+TGG	0.294063	2.4:+62034055	None:intergenic
GGGAGTGGATCGCCATCTTC+TGG	0.311375	2.4:-62033659	MS.gene02911:CDS
CGTGAGTAGGATCATGAAAA+AGG	0.331544	2.4:-62034044	MS.gene02911:CDS
GGACATAATATGTATGGATC+TGG	0.358939	2.4:-62033680	MS.gene02911:CDS
GAGTTCATCAGTTTCATAAC+AGG	0.366706	2.4:-62033959	MS.gene02911:CDS
CTTTGGGCTATGACAACTCT+TGG	0.376889	2.4:-62033878	MS.gene02911:CDS
CAACGGTGATGATCTTCTTT+GGG	0.389396	2.4:-62033894	MS.gene02911:CDS
CAGGTTGCAGAGAGCAAGAT+AGG	0.404952	2.4:-62034084	MS.gene02911:CDS
GGAGAGAAGAGTGCTATGAT+TGG	0.411557	2.4:-62033797	MS.gene02911:CDS
AGTGGTGGAGTTTATGGTCA+TGG	0.452362	2.4:-62033737	MS.gene02911:CDS
GGAGTTTATGGTCATGGAGT+TGG	0.458217	2.4:-62033731	MS.gene02911:CDS
CGAGTACTCCTTCCAGAAGA+TGG	0.478915	2.4:+62033647	None:intergenic
AGAAGAGTGCTATGATTGGT+AGG	0.483869	2.4:-62033793	MS.gene02911:CDS
ATCATCATCATCATAGAAGA+TGG	0.485382	2.4:+62033704	None:intergenic
ATCTTCTATGATGATGATGA+TGG	0.489435	2.4:-62033702	MS.gene02911:CDS
GGTAGGAGTGATCAGAGAGA+TGG	0.493822	2.4:-62033776	MS.gene02911:CDS
GGAAATGTTGGTGAGGGTAG+TGG	0.495110	2.4:-62033755	MS.gene02911:CDS
TTTGAGTAAGTATAGAGAGA+TGG	0.496858	2.4:-62033822	MS.gene02911:CDS
ATGATGGGACATAATATGTA+TGG	0.500614	2.4:-62033686	MS.gene02911:CDS
GATCAGAGAGATGGAAATGT+TGG	0.512395	2.4:-62033767	MS.gene02911:CDS
GACATAATATGTATGGATCT+GGG	0.545156	2.4:-62033679	MS.gene02911:CDS
AGAGATGGAAATGTTGGTGA+GGG	0.549342	2.4:-62033761	MS.gene02911:CDS
GAATCAGACAATGAGTCAGC+AGG	0.557061	2.4:-62034103	MS.gene02911:CDS
ATAAGTGTCAGAAAGAGAAG+AGG	0.567471	2.4:-62033925	MS.gene02911:CDS
AGTAAGTATAGAGAGATGGA+AGG	0.568189	2.4:-62033818	MS.gene02911:CDS
AATGTTGGTGAGGGTAGTGG+TGG	0.579830	2.4:-62033752	MS.gene02911:CDS
AATATGTATGGATCTGGGAG+TGG	0.582558	2.4:-62033674	MS.gene02911:CDS
TTCCAATAGCAAACGTGAGT+AGG	0.595976	2.4:-62034057	MS.gene02911:CDS
TTACTCAAATAAATCTTGAG+TGG	0.610816	2.4:+62033836	None:intergenic
GAGAAGAGGAAAACAATCAA+CGG	0.615125	2.4:-62033911	MS.gene02911:CDS
GAGAGATGGAAATGTTGGTG+AGG	0.620088	2.4:-62033762	MS.gene02911:CDS
GTGGATCGCCATCTTCTGGA+AGG	0.622809	2.4:-62033655	MS.gene02911:CDS
TCTTCTATGATGATGATGAT+GGG	0.641124	2.4:-62033701	MS.gene02911:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTACTCAAATAAATCTTGAG+TGG	+	chr2.4:62033919-62033938	None:intergenic	25.0%
	ATCATCATCATCATAGAAGA+TGG	+	chr2.4:62034051-62034070	None:intergenic	30.0%
	GACATAATATGTATGGATCT+GGG	-	chr2.4:62034073-62034092	MS.gene02911:CDS	30.0%
	TTTGAGTAAGTATAGAGAGA+TGG	-	chr2.4:62033930-62033949	MS.gene02911:CDS	30.0%
!	ATCTTCTATGATGATGATGA+TGG	-	chr2.4:62034050-62034069	MS.gene02911:CDS	30.0%
!	ATGATGGGACATAATATGTA+TGG	-	chr2.4:62034066-62034085	MS.gene02911:CDS	30.0%
!	TCTTCTATGATGATGATGAT+GGG	-	chr2.4:62034051-62034070	MS.gene02911:CDS	30.0%
	AGTAAGTATAGAGAGATGGA+AGG	-	chr2.4:62033934-62033953	MS.gene02911:CDS	35.0%
	ATAAGTGTCAGAAAGAGAAG+AGG	-	chr2.4:62033827-62033846	MS.gene02911:CDS	35.0%
	GAGAAGAGGAAAACAATCAA+CGG	-	chr2.4:62033841-62033860	MS.gene02911:CDS	35.0%
	GAGTTCATCAGTTTCATAAC+AGG	-	chr2.4:62033793-62033812	MS.gene02911:CDS	35.0%
	GGACATAATATGTATGGATC+TGG	-	chr2.4:62034072-62034091	MS.gene02911:CDS	35.0%
	AATATGTATGGATCTGGGAG+TGG	-	chr2.4:62034078-62034097	MS.gene02911:CDS	40.0%
	CAACGGTGATGATCTTCTTT+GGG	-	chr2.4:62033858-62033877	MS.gene02911:CDS	40.0%
	CGTGAGTAGGATCATGAAAA+AGG	-	chr2.4:62033708-62033727	MS.gene02911:CDS	40.0%
	GATCAGAGAGATGGAAATGT+TGG	-	chr2.4:62033985-62034004	MS.gene02911:CDS	40.0%
	TCAACGGTGATGATCTTCTT+TGG	-	chr2.4:62033857-62033876	MS.gene02911:CDS	40.0%
	TTCCAATAGCAAACGTGAGT+AGG	-	chr2.4:62033695-62033714	MS.gene02911:CDS	40.0%
!	AGAGATGGAAATGTTGGTGA+GGG	-	chr2.4:62033991-62034010	MS.gene02911:CDS	40.0%
!	TTTGAGATCTTTGCGTTTGC+TGG	+	chr2.4:62033739-62033758	None:intergenic	40.0%
!!	AGAAGAGTGCTATGATTGGT+AGG	-	chr2.4:62033959-62033978	MS.gene02911:CDS	40.0%
!!	ATCCTACTCACGTTTGCTAT+TGG	+	chr2.4:62033700-62033719	None:intergenic	40.0%
	CTTTGGGCTATGACAACTCT+TGG	-	chr2.4:62033874-62033893	MS.gene02911:CDS	45.0%
	GAATCAGACAATGAGTCAGC+AGG	-	chr2.4:62033649-62033668	MS.gene02911:CDS	45.0%
	GAGAGATGGAAATGTTGGTG+AGG	-	chr2.4:62033990-62034009	MS.gene02911:CDS	45.0%
	GGAGTTTATGGTCATGGAGT+TGG	-	chr2.4:62034021-62034040	MS.gene02911:CDS	45.0%
!	AGTGGTGGAGTTTATGGTCA+TGG	-	chr2.4:62034015-62034034	MS.gene02911:CDS	45.0%
!!	GGAGAGAAGAGTGCTATGAT+TGG	-	chr2.4:62033955-62033974	MS.gene02911:CDS	45.0%
	CAGGTTGCAGAGAGCAAGAT+AGG	-	chr2.4:62033668-62033687	MS.gene02911:CDS	50.0%
	CGAGTACTCCTTCCAGAAGA+TGG	+	chr2.4:62034108-62034127	None:intergenic	50.0%
	GGTAGGAGTGATCAGAGAGA+TGG	-	chr2.4:62033976-62033995	MS.gene02911:CDS	50.0%
!	AATGTTGGTGAGGGTAGTGG+TGG	-	chr2.4:62034000-62034019	MS.gene02911:CDS	50.0%
!	GAGGGTAGTGGTGGAGTTTA+TGG	-	chr2.4:62034009-62034028	MS.gene02911:CDS	50.0%
!	GGAAATGTTGGTGAGGGTAG+TGG	-	chr2.4:62033997-62034016	MS.gene02911:CDS	50.0%
	GTGGATCGCCATCTTCTGGA+AGG	-	chr2.4:62034097-62034116	MS.gene02911:CDS	55.0%
	GGGAGTGGATCGCCATCTTC+TGG	-	chr2.4:62034093-62034112	MS.gene02911:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	62033643	62034131	62033643	ID=MS.gene02911
chr2.4	mRNA	62033643	62034131	62033643	ID=MS.gene02911.t1;Parent=MS.gene02911
chr2.4	exon	62033643	62034131	62033643	ID=MS.gene02911.t1.exon1;Parent=MS.gene02911.t1
chr2.4	CDS	62033643	62034131	62033643	ID=cds.MS.gene02911.t1;Parent=MS.gene02911.t1

Gene Sequence

>MS.gene02911

ATGGCTGAATCAGACAATGAGTCAGCAGGTTGCAGAGAGCAAGATAGGTTTCTTCCAATAGCAAACGTGAGTAGGATCATGAAAAAGGCTTTACCAGCAAACGCAAAGATCTCAAAAGAAGCAAAAGAAACAGTGCAAGAGTGTGTATCAGAGTTCATCAGTTTCATAACAGGAGAAGCTTCTGATAAGTGTCAGAAAGAGAAGAGGAAAACAATCAACGGTGATGATCTTCTTTGGGCTATGACAACTCTTGGATTTGAAGATTATGTTGAGCCACTCAAGATTTATTTGAGTAAGTATAGAGAGATGGAAGGAGAGAAGAGTGCTATGATTGGTAGGAGTGATCAGAGAGATGGAAATGTTGGTGAGGGTAGTGGTGGAGTTTATGGTCATGGAGTTGGAGTTCCATCTTCTATGATGATGATGATGGGACATAATATGTATGGATCTGGGAGTGGATCGCCATCTTCTGGAAGGAGTACTCGATAG

Protein sequence

>MS.gene02911.t1

MAESDNESAGCREQDRFLPIANVSRIMKKALPANAKISKEAKETVQECVSEFISFITGEASDKCQKEKRKTINGDDLLWAMTTLGFEDYVEPLKIYLSKYREMEGEKSAMIGRSDQRDGNVGEGSGGVYGHGVGVPSSMMMMMGHNMYGSGSGSPSSGRSTR