Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029291.t1 | XP_013470321.1 | 96.8 | 466 | 15 | 0 | 6 | 471 | 2 | 467 | 5.10E-277 | 963 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029291.t1 | Q09131 | 83.2 | 471 | 72 | 2 | 1 | 471 | 1 | 464 | 8.9e-246 | 850.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029291.t1 | A0A072VQW8 | 96.8 | 466 | 15 | 0 | 6 | 471 | 2 | 467 | 3.7e-277 | 963.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene029291 | MS.gene58017 | PPI |
| MS.gene029291 | MS.gene58016 | PPI |
| MS.gene029291 | MS.gene58221 | PPI |
| MS.gene029291 | MS.gene036701 | PPI |
| MS.gene029291 | MS.gene58198 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029291.t1 | MTR_1g112630 | 96.781 | 466 | 15 | 0 | 6 | 471 | 2 | 467 | 0.0 | 945 |
| MS.gene029291.t1 | MTR_4g037105 | 91.537 | 449 | 37 | 1 | 23 | 471 | 18 | 465 | 0.0 | 866 |
| MS.gene029291.t1 | MTR_0188s0020 | 83.929 | 448 | 71 | 1 | 24 | 471 | 25 | 471 | 0.0 | 799 |
| MS.gene029291.t1 | MTR_4g115670 | 59.815 | 433 | 170 | 3 | 28 | 459 | 23 | 452 | 0.0 | 543 |
| MS.gene029291.t1 | MTR_3g051240 | 43.035 | 402 | 198 | 10 | 54 | 454 | 39 | 410 | 1.42e-95 | 295 |
| MS.gene029291.t1 | MTR_5g092360 | 41.452 | 427 | 216 | 12 | 30 | 454 | 22 | 416 | 3.61e-95 | 295 |
| MS.gene029291.t1 | MTR_1g060360 | 41.919 | 396 | 202 | 10 | 60 | 454 | 46 | 414 | 1.29e-91 | 285 |
| MS.gene029291.t1 | MTR_7g104330 | 41.814 | 397 | 201 | 10 | 60 | 454 | 54 | 422 | 1.87e-91 | 285 |
| MS.gene029291.t1 | MTR_7g104360 | 40.250 | 400 | 208 | 11 | 58 | 454 | 45 | 416 | 1.29e-87 | 275 |
| MS.gene029291.t1 | MTR_6g013050 | 31.028 | 535 | 261 | 15 | 7 | 449 | 1 | 519 | 1.25e-72 | 240 |
| MS.gene029291.t1 | MTR_3g083810 | 37.215 | 395 | 173 | 9 | 115 | 450 | 143 | 521 | 6.96e-72 | 237 |
| MS.gene029291.t1 | MTR_3g083820 | 37.215 | 395 | 173 | 9 | 115 | 450 | 143 | 521 | 9.66e-71 | 234 |
| MS.gene029291.t1 | MTR_7g056403 | 35.581 | 430 | 194 | 12 | 87 | 450 | 108 | 520 | 1.12e-70 | 234 |
| MS.gene029291.t1 | MTR_6g013050 | 30.291 | 515 | 251 | 15 | 7 | 429 | 1 | 499 | 8.98e-65 | 218 |
| MS.gene029291.t1 | MTR_1g052505 | 26.225 | 408 | 211 | 15 | 52 | 397 | 183 | 562 | 3.82e-29 | 121 |
| MS.gene029291.t1 | MTR_4g037670 | 27.895 | 380 | 202 | 15 | 55 | 397 | 237 | 581 | 5.52e-28 | 117 |
| MS.gene029291.t1 | MTR_4g082930 | 24.573 | 468 | 271 | 16 | 39 | 444 | 125 | 572 | 7.08e-21 | 96.3 |
| MS.gene029291.t1 | MTR_3g074930 | 25.627 | 359 | 220 | 16 | 119 | 444 | 256 | 600 | 1.90e-19 | 91.7 |
| MS.gene029291.t1 | MTR_3g074930 | 25.627 | 359 | 220 | 16 | 119 | 444 | 249 | 593 | 1.97e-19 | 91.7 |
| MS.gene029291.t1 | MTR_4g103520 | 25.915 | 355 | 224 | 13 | 119 | 444 | 176 | 520 | 6.36e-19 | 89.7 |
| MS.gene029291.t1 | MTR_4g103520 | 25.915 | 355 | 224 | 13 | 119 | 444 | 253 | 597 | 6.52e-19 | 90.1 |
| MS.gene029291.t1 | MTR_5g045600 | 25.405 | 370 | 207 | 14 | 119 | 444 | 245 | 589 | 1.43e-18 | 89.0 |
| MS.gene029291.t1 | MTR_8g063080 | 24.054 | 370 | 212 | 15 | 119 | 444 | 238 | 582 | 5.52e-17 | 84.0 |
| MS.gene029291.t1 | MTR_4g082940 | 25.424 | 236 | 149 | 8 | 227 | 444 | 332 | 558 | 3.33e-13 | 72.4 |
| MS.gene029291.t1 | MTR_3g074970 | 23.276 | 348 | 197 | 12 | 119 | 444 | 198 | 497 | 1.42e-11 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene029291.t1 | AT2G16430 | 75.484 | 465 | 112 | 2 | 1 | 465 | 1 | 463 | 0.0 | 749 |
| MS.gene029291.t1 | AT2G27190 | 67.756 | 459 | 147 | 1 | 1 | 459 | 1 | 458 | 0.0 | 662 |
| MS.gene029291.t1 | AT1G56360 | 63.274 | 452 | 158 | 4 | 18 | 464 | 10 | 458 | 0.0 | 601 |
| MS.gene029291.t1 | AT2G16430 | 80.826 | 339 | 65 | 0 | 129 | 467 | 7 | 345 | 0.0 | 599 |
| MS.gene029291.t1 | AT4G36350 | 61.842 | 456 | 167 | 4 | 13 | 464 | 6 | 458 | 0.0 | 593 |
| MS.gene029291.t1 | AT5G34850 | 56.828 | 454 | 189 | 4 | 7 | 459 | 4 | 451 | 0.0 | 540 |
| MS.gene029291.t1 | AT2G18130 | 55.580 | 457 | 177 | 6 | 13 | 466 | 8 | 441 | 0.0 | 523 |
| MS.gene029291.t1 | AT3G46120 | 46.957 | 460 | 158 | 10 | 6 | 459 | 2 | 381 | 1.60e-135 | 396 |
| MS.gene029291.t1 | AT1G52940 | 61.789 | 246 | 86 | 6 | 44 | 286 | 1 | 241 | 7.41e-106 | 315 |
| MS.gene029291.t1 | AT3G52820 | 43.182 | 396 | 197 | 11 | 60 | 454 | 47 | 415 | 8.77e-94 | 291 |
| MS.gene029291.t1 | AT3G20500 | 42.462 | 398 | 200 | 9 | 58 | 454 | 45 | 414 | 1.62e-92 | 288 |
| MS.gene029291.t1 | AT3G52810 | 39.421 | 449 | 234 | 12 | 5 | 450 | 2 | 415 | 2.94e-90 | 282 |
| MS.gene029291.t1 | AT3G52780 | 38.789 | 446 | 232 | 12 | 16 | 454 | 5 | 416 | 7.32e-86 | 270 |
| MS.gene029291.t1 | AT2G32770 | 34.353 | 425 | 189 | 10 | 100 | 450 | 122 | 530 | 2.51e-67 | 225 |
| MS.gene029291.t1 | AT3G07130 | 33.929 | 448 | 206 | 12 | 77 | 450 | 80 | 511 | 1.65e-66 | 223 |
| MS.gene029291.t1 | AT4G13700 | 35.561 | 374 | 183 | 11 | 77 | 408 | 80 | 437 | 6.71e-61 | 206 |
| MS.gene029291.t1 | AT3G07130 | 31.601 | 481 | 206 | 13 | 77 | 450 | 80 | 544 | 7.63e-60 | 206 |
| MS.gene029291.t1 | AT2G32770 | 33.002 | 403 | 180 | 10 | 100 | 428 | 41 | 427 | 9.34e-59 | 199 |
| MS.gene029291.t1 | AT3G52780 | 36.096 | 374 | 207 | 12 | 16 | 380 | 5 | 355 | 2.80e-56 | 191 |
| MS.gene029291.t1 | AT2G32770 | 31.294 | 425 | 173 | 11 | 100 | 450 | 122 | 501 | 1.46e-53 | 188 |
| MS.gene029291.t1 | AT4G13700 | 35.366 | 328 | 161 | 11 | 77 | 360 | 80 | 400 | 5.34e-49 | 173 |
| MS.gene029291.t1 | AT1G13900 | 25.108 | 462 | 258 | 17 | 47 | 444 | 132 | 569 | 3.10e-23 | 103 |
| MS.gene029291.t1 | AT2G03450 | 25.111 | 450 | 253 | 16 | 58 | 444 | 141 | 569 | 7.16e-20 | 93.2 |
| MS.gene029291.t1 | AT5G50400 | 25.281 | 356 | 225 | 14 | 119 | 444 | 165 | 509 | 8.66e-20 | 92.4 |
| MS.gene029291.t1 | AT5G50400 | 25.281 | 356 | 225 | 14 | 119 | 444 | 237 | 581 | 1.08e-19 | 92.4 |
| MS.gene029291.t1 | AT1G13750 | 25.549 | 364 | 214 | 14 | 119 | 444 | 239 | 583 | 1.52e-17 | 85.9 |
| MS.gene029291.t1 | AT4G24890 | 28.467 | 274 | 163 | 11 | 119 | 364 | 241 | 509 | 2.20e-17 | 85.5 |
Find 110 sgRNAs with CRISPR-Local
Find 127 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTTGGTTTCGCCAATTTC+TGG | 0.125859 | 1.4:+86511436 | None:intergenic |
| TCACACTACTATAAGGAATT+TGG | 0.199886 | 1.4:-86511794 | MS.gene029291:CDS |
| CACCTAACATTGTCATGATT+TGG | 0.223594 | 1.4:+86511544 | None:intergenic |
| ATTGATTTCGCCCCAGAAAT+TGG | 0.247783 | 1.4:-86511448 | MS.gene029291:CDS |
| GTACTTTGTGATGCTTTATA+AGG | 0.272518 | 1.4:+86511382 | None:intergenic |
| GGCACATCAGGACCAATTTC+AGG | 0.281679 | 1.4:+86511700 | None:intergenic |
| TTTATAACTCCTCCTGAAAT+TGG | 0.285563 | 1.4:-86511712 | MS.gene029291:CDS |
| CACACGTTCGGTTCTTTCAT+AGG | 0.290239 | 1.4:+86511081 | None:intergenic |
| TAGCAACAATCAACATTATA+TGG | 0.299454 | 1.4:-86511185 | MS.gene029291:CDS |
| CACAAAGTACTTCACCCTTT+TGG | 0.304317 | 1.4:-86511369 | MS.gene029291:CDS |
| TTATTCATCACACTACTATA+AGG | 0.305033 | 1.4:-86511801 | MS.gene029291:CDS |
| GTGATGCTTTATAAGGAGTA+TGG | 0.325433 | 1.4:+86511389 | None:intergenic |
| GTTTCTGTCCTGTTAACTTT+AGG | 0.327863 | 1.4:+86511247 | None:intergenic |
| TGGCTCATCAACCATGGATA+TGG | 0.337293 | 1.4:-86511489 | MS.gene029291:CDS |
| CTGGGGCGAAATCAATTTCA+TGG | 0.339164 | 1.4:+86511455 | None:intergenic |
| GGAACACAACAAGACAGTTT+TGG | 0.351168 | 1.4:-86511735 | MS.gene029291:CDS |
| AGATTCTTCAATTACTCATC+TGG | 0.355881 | 1.4:-86511826 | MS.gene029291:CDS |
| GTTTGGTTTCGCCAATTTCT+GGG | 0.356914 | 1.4:+86511437 | None:intergenic |
| TACTATTATGAGGTTGGACT+TGG | 0.361764 | 1.4:-86511757 | MS.gene029291:CDS |
| ACCTAACATTGTCATGATTT+GGG | 0.368249 | 1.4:+86511545 | None:intergenic |
| GGTCGAACGCAGTGCGCTAC+TGG | 0.369778 | 1.4:-86511903 | MS.gene029291:CDS |
| ACAATGTTAGGTGGGATACT+TGG | 0.374862 | 1.4:-86511534 | MS.gene029291:CDS |
| CGAATAAGGTTTGAATGGTT+TGG | 0.378529 | 1.4:+86511420 | None:intergenic |
| GGGAAAACAGTGTTGTTTGT+TGG | 0.380568 | 1.4:-86511592 | MS.gene029291:CDS |
| AATTTGGTGTTTGTGTGTAA+TGG | 0.386262 | 1.4:-86512099 | MS.gene029291:CDS |
| GTATGGTATCGGTGCGAATA+AGG | 0.387101 | 1.4:+86511406 | None:intergenic |
| AGGAGCTACCTAAAGTTAAC+AGG | 0.390499 | 1.4:-86511255 | MS.gene029291:CDS |
| CAGAAATCAAGATGGTTATT+CGG | 0.397907 | 1.4:-86510843 | MS.gene029291:CDS |
| TGCAATATTCGACACACGTT+CGG | 0.401173 | 1.4:+86511069 | None:intergenic |
| TTTGGTCTCATAGGAGATCT+TGG | 0.404619 | 1.4:-86511670 | MS.gene029291:CDS |
| CCAAATGTGTATGGCACATC+AGG | 0.405903 | 1.4:+86511688 | None:intergenic |
| GCATTCAGAGAGGCTAGCTT+TGG | 0.414506 | 1.4:-86510917 | MS.gene029291:CDS |
| TGGTTATAATGCTCCCCAAC+AGG | 0.417531 | 1.4:-86511995 | MS.gene029291:CDS |
| CCATACACATTTGGTCTCAT+AGG | 0.418182 | 1.4:-86511679 | MS.gene029291:CDS |
| TCCCAAATCATGACAATGTT+AGG | 0.419243 | 1.4:-86511546 | MS.gene029291:CDS |
| TTCAGCTCACATCATTGTCT+TGG | 0.421163 | 1.4:-86511329 | MS.gene029291:CDS |
| CCTGATGTGCCATACACATT+TGG | 0.422920 | 1.4:-86511688 | MS.gene029291:CDS |
| CGGTGCGAATAAGGTTTGAA+TGG | 0.426529 | 1.4:+86511415 | None:intergenic |
| TCTCCAATGGTTATGTATAC+AGG | 0.430699 | 1.4:+86510998 | None:intergenic |
| TTACACAAGGTGATCTTGTT+GGG | 0.430973 | 1.4:-86511966 | MS.gene029291:CDS |
| TGAGCGGTGGAATCATCAAC+TGG | 0.432170 | 1.4:+86510779 | None:intergenic |
| TTGGCCTCGTATTCAGCATA+TGG | 0.441264 | 1.4:-86511310 | MS.gene029291:CDS |
| CAATGTTAGGTGGGATACTT+GGG | 0.445086 | 1.4:-86511533 | MS.gene029291:CDS |
| ATTACACAAGGTGATCTTGT+TGG | 0.449022 | 1.4:-86511967 | MS.gene029291:CDS |
| TGGCTGTGGTTCTGTCATGT+TGG | 0.470647 | 1.4:+86510949 | None:intergenic |
| CGGTTGAGGCTGATTCCCAT+TGG | 0.478638 | 1.4:-86510823 | MS.gene029291:CDS |
| CTTGGGGAAGGTTTGTAGAA+AGG | 0.482123 | 1.4:-86511516 | MS.gene029291:CDS |
| ACAGTACCAATGGCTTGAAC+AGG | 0.482376 | 1.4:-86511275 | MS.gene029291:CDS |
| ACTAAATACTATTATGAGGT+TGG | 0.489189 | 1.4:-86511763 | MS.gene029291:CDS |
| GGAGATGGAGGGAACCTTGG+AGG | 0.490799 | 1.4:-86510980 | MS.gene029291:CDS |
| GTAGCTCCTGTTCAAGCCAT+TGG | 0.495291 | 1.4:+86511269 | None:intergenic |
| TTGTGTAATATGAACCTGTT+GGG | 0.497585 | 1.4:+86511981 | None:intergenic |
| CCTATGAGACCAAATGTGTA+TGG | 0.500434 | 1.4:+86511679 | None:intergenic |
| AAGGTTGATGTTGTGTATGC+TGG | 0.513119 | 1.4:-86511115 | MS.gene029291:CDS |
| TTAACAGGACAGAAACTCCT+TGG | 0.513520 | 1.4:-86511240 | MS.gene029291:CDS |
| TCAAGATGGTTATTCGGTTG+AGG | 0.514507 | 1.4:-86510837 | MS.gene029291:CDS |
| GCCATGGTTTGTTAAGTACA+AGG | 0.515158 | 1.4:-86511134 | MS.gene029291:CDS |
| ATTGGAGATGGAGGGAACCT+TGG | 0.515906 | 1.4:-86510983 | MS.gene029291:CDS |
| TAGCGCACTGCGTTCGACCC+TGG | 0.519503 | 1.4:+86511907 | None:intergenic |
| AAACAAATGGGTATTTCTCA+AGG | 0.519830 | 1.4:-86512165 | None:intergenic |
| GAACACATGCTCACTATAGC+TGG | 0.521332 | 1.4:-86510868 | MS.gene029291:CDS |
| TGGGTGACCGAGGATGAACC+AGG | 0.523413 | 1.4:-86511925 | MS.gene029291:CDS |
| AACAATCAACATTATATGGA+AGG | 0.525860 | 1.4:-86511181 | MS.gene029291:CDS |
| GTTCGACCCTGGTTCATCCT+CGG | 0.543679 | 1.4:+86511918 | None:intergenic |
| ATTGCATATAATGTTGTGAA+TGG | 0.545809 | 1.4:-86511052 | MS.gene029291:CDS |
| TACATAACCATTGGAGATGG+AGG | 0.550025 | 1.4:-86510992 | MS.gene029291:CDS |
| CAACACTAAATACTATTATG+AGG | 0.552300 | 1.4:-86511767 | MS.gene029291:CDS |
| GCTCCTGTATACATAACCAT+TGG | 0.553714 | 1.4:-86511001 | MS.gene029291:CDS |
| ACAATCAACATTATATGGAA+GGG | 0.554284 | 1.4:-86511180 | MS.gene029291:CDS |
| TCTCTGAATGCTGAGTACTC+TGG | 0.561355 | 1.4:+86510929 | None:intergenic |
| AATGCTGAGTACTCTGGCTG+TGG | 0.569296 | 1.4:+86510935 | None:intergenic |
| CTTTATAAGGAGTATGGTAT+CGG | 0.573117 | 1.4:+86511395 | None:intergenic |
| TTTCCTACAGTCCATATCCA+TGG | 0.573550 | 1.4:+86511478 | None:intergenic |
| CAAATCATGACAATGTTAGG+TGG | 0.575920 | 1.4:-86511543 | MS.gene029291:CDS |
| TGTTGGTTTCTAAGCCTCCA+AGG | 0.576998 | 1.4:+86510966 | None:intergenic |
| CAATCAACATTATATGGAAG+GGG | 0.577345 | 1.4:-86511179 | MS.gene029291:CDS |
| CAACCATGGATATGGACTGT+AGG | 0.580717 | 1.4:-86511481 | MS.gene029291:CDS |
| AATATACACCACAGTACCAA+TGG | 0.587307 | 1.4:-86511285 | MS.gene029291:CDS |
| TGTTCAAGCCATTGGTACTG+TGG | 0.587686 | 1.4:+86511277 | None:intergenic |
| GTATACATAACCATTGGAGA+TGG | 0.590351 | 1.4:-86510995 | MS.gene029291:CDS |
| TTGGTGTTTGTGTGTAATGG+AGG | 0.590798 | 1.4:-86512096 | MS.gene029291:CDS |
| GATCGTATCGTGGGTGACCG+AGG | 0.591550 | 1.4:-86511935 | MS.gene029291:CDS |
| TTAGGTGGGATACTTGGGGA+AGG | 0.593341 | 1.4:-86511528 | MS.gene029291:CDS |
| AAGGTTCCCTCCATCTCCAA+TGG | 0.594309 | 1.4:+86510985 | None:intergenic |
| AAATCATGACAATGTTAGGT+GGG | 0.598863 | 1.4:-86511542 | MS.gene029291:CDS |
| GGAAAGCAGTGATCGTATCG+TGG | 0.602783 | 1.4:-86511945 | MS.gene029291:CDS |
| ACTATTATGAGGTTGGACTT+GGG | 0.609032 | 1.4:-86511756 | MS.gene029291:CDS |
| TTTGGTTTCGCCAATTTCTG+GGG | 0.609945 | 1.4:+86511438 | None:intergenic |
| TTTGTTAGGAATGTTCAGAA+GGG | 0.615218 | 1.4:-86512060 | MS.gene029291:CDS |
| ACCTTGTACTTAACAAACCA+TGG | 0.616531 | 1.4:+86511133 | None:intergenic |
| TGAGAGTAATGTATGAGCCA+TGG | 0.618209 | 1.4:-86511150 | MS.gene029291:CDS |
| GAAAGCAGTGATCGTATCGT+GGG | 0.619977 | 1.4:-86511944 | MS.gene029291:CDS |
| GGAGCGTGGCTCATCAACCA+TGG | 0.634638 | 1.4:-86511495 | MS.gene029291:CDS |
| AGCTGGCACAGAAATCAAGA+TGG | 0.636064 | 1.4:-86510851 | MS.gene029291:CDS |
| TCAATGGAAAACATGAGCGG+TGG | 0.642932 | 1.4:+86510766 | None:intergenic |
| TGCATTAGAACAATCAACCA+AGG | 0.650420 | 1.4:+86511223 | None:intergenic |
| CTTGTGTAATATGAACCTGT+TGG | 0.651645 | 1.4:+86511980 | None:intergenic |
| ACATAACCATTGGAGATGGA+GGG | 0.652593 | 1.4:-86510991 | MS.gene029291:CDS |
| TGGGGAGCATTATAACCAGA+AGG | 0.658702 | 1.4:+86512000 | None:intergenic |
| CAACAGGTTCATATTACACA+AGG | 0.659532 | 1.4:-86511979 | MS.gene029291:CDS |
| TTGTTCTAATGCATTCACCA+TGG | 0.660660 | 1.4:-86511213 | MS.gene029291:CDS |
| GAGCGGTGGAATCATCAACT+GGG | 0.687131 | 1.4:+86510780 | None:intergenic |
| ACATCAGGACCAATTTCAGG+AGG | 0.690189 | 1.4:+86511703 | None:intergenic |
| TGATTGTTGCTATTATACCA+TGG | 0.695794 | 1.4:+86511196 | None:intergenic |
| AAGGTTTGTAGAAAGGAGCG+TGG | 0.697817 | 1.4:-86511509 | MS.gene029291:CDS |
| GGGTGACCGAGGATGAACCA+GGG | 0.713113 | 1.4:-86511924 | MS.gene029291:CDS |
| AGAGTACTCAGCATTCAGAG+AGG | 0.715627 | 1.4:-86510927 | MS.gene029291:CDS |
| GTATCAATGGAAAACATGAG+CGG | 0.720779 | 1.4:+86510763 | None:intergenic |
| AATGTTAGGTGGGATACTTG+GGG | 0.732363 | 1.4:-86511532 | MS.gene029291:CDS |
| TGTGTAATATGAACCTGTTG+GGG | 0.733638 | 1.4:+86511982 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATCTGTTGAAAAACCAA+TGG | + | chr1.4:86512121-86512140 | None:intergenic | 25.0% |
| ! | AAACAAAAAAAGCTAGCTAA+AGG | - | chr1.4:86511060-86511079 | MS.gene029291:CDS | 25.0% |
| ! | AAATCTGTTGAAAAACCAAT+GGG | + | chr1.4:86512120-86512139 | None:intergenic | 25.0% |
| ! | AACAAAAAAAGCTAGCTAAA+GGG | - | chr1.4:86511061-86511080 | MS.gene029291:CDS | 25.0% |
| ! | AACAATCAACATTATATGGA+AGG | - | chr1.4:86511744-86511763 | MS.gene029291:CDS | 25.0% |
| ! | ACAATCAACATTATATGGAA+GGG | - | chr1.4:86511745-86511764 | MS.gene029291:CDS | 25.0% |
| ! | ACTAAATACTATTATGAGGT+TGG | - | chr1.4:86511162-86511181 | MS.gene029291:CDS | 25.0% |
| ! | ATGAATTGAATCCCAAAAAA+GGG | - | chr1.4:86511313-86511332 | MS.gene029291:CDS | 25.0% |
| ! | ATTGCATATAATGTTGTGAA+TGG | - | chr1.4:86511873-86511892 | MS.gene029291:CDS | 25.0% |
| ! | CAACACTAAATACTATTATG+AGG | - | chr1.4:86511158-86511177 | MS.gene029291:CDS | 25.0% |
| ! | TAGCAACAATCAACATTATA+TGG | - | chr1.4:86511740-86511759 | MS.gene029291:CDS | 25.0% |
| ! | TATGAATTGAATCCCAAAAA+AGG | - | chr1.4:86511312-86511331 | MS.gene029291:CDS | 25.0% |
| ! | TCTGTTCATTATGTCAAAAA+TGG | + | chr1.4:86512039-86512058 | None:intergenic | 25.0% |
| ! | TTATTCATCACACTACTATA+AGG | - | chr1.4:86511124-86511143 | MS.gene029291:CDS | 25.0% |
| !! | CTTTTGATAGAATACCAAAA+GGG | + | chr1.4:86511573-86511592 | None:intergenic | 25.0% |
| !! | TCTTTTGATAGAATACCAAA+AGG | + | chr1.4:86511574-86511593 | None:intergenic | 25.0% |
| !!! | ATTGGTTTTTCAACAGATTT+TGG | - | chr1.4:86512120-86512139 | MS.gene029291:CDS | 25.0% |
| !!! | TTTAGCTAGCTTTTTTTGTT+TGG | + | chr1.4:86511061-86511080 | None:intergenic | 25.0% |
| AAATCATGACAATGTTAGGT+GGG | - | chr1.4:86511383-86511402 | MS.gene029291:CDS | 30.0% | |
| ACCTAACATTGTCATGATTT+GGG | + | chr1.4:86511383-86511402 | None:intergenic | 30.0% | |
| AGATTCTTCAATTACTCATC+TGG | - | chr1.4:86511099-86511118 | MS.gene029291:CDS | 30.0% | |
| CAATCAACATTATATGGAAG+GGG | - | chr1.4:86511746-86511765 | MS.gene029291:CDS | 30.0% | |
| CAGAAATCAAGATGGTTATT+CGG | - | chr1.4:86512082-86512101 | MS.gene029291:CDS | 30.0% | |
| GTACTTTGTGATGCTTTATA+AGG | + | chr1.4:86511546-86511565 | None:intergenic | 30.0% | |
| TCACACTACTATAAGGAATT+TGG | - | chr1.4:86511131-86511150 | MS.gene029291:CDS | 30.0% | |
| TTGTGTAATATGAACCTGTT+GGG | + | chr1.4:86510947-86510966 | None:intergenic | 30.0% | |
| TTTATAACTCCTCCTGAAAT+TGG | - | chr1.4:86511213-86511232 | MS.gene029291:CDS | 30.0% | |
| TTTGTTAGGAATGTTCAGAA+GGG | - | chr1.4:86510865-86510884 | MS.gene029291:CDS | 30.0% | |
| ! | AACACTGTTTTCCCTTTTTT+GGG | + | chr1.4:86511327-86511346 | None:intergenic | 30.0% |
| ! | CTTTATAAGGAGTATGGTAT+CGG | + | chr1.4:86511533-86511552 | None:intergenic | 30.0% |
| ! | TGATTGTTGCTATTATACCA+TGG | + | chr1.4:86511732-86511751 | None:intergenic | 30.0% |
| ! | TTTTGTTAGGAATGTTCAGA+AGG | - | chr1.4:86510864-86510883 | MS.gene029291:CDS | 30.0% |
| !! | AATTTGGTGTTTGTGTGTAA+TGG | - | chr1.4:86510826-86510845 | MS.gene029291:CDS | 30.0% |
| !!! | ACTAGCTCTAGTTTTGAATT+TGG | - | chr1.4:86510810-86510829 | MS.gene029291:CDS | 30.0% |
| AATATACACCACAGTACCAA+TGG | - | chr1.4:86511640-86511659 | MS.gene029291:CDS | 35.0% | |
| ACCTTGTACTTAACAAACCA+TGG | + | chr1.4:86511795-86511814 | None:intergenic | 35.0% | |
| ACTATTATGAGGTTGGACTT+GGG | - | chr1.4:86511169-86511188 | MS.gene029291:CDS | 35.0% | |
| ATTACACAAGGTGATCTTGT+TGG | - | chr1.4:86510958-86510977 | MS.gene029291:CDS | 35.0% | |
| CAAATCATGACAATGTTAGG+TGG | - | chr1.4:86511382-86511401 | MS.gene029291:CDS | 35.0% | |
| CAACAGGTTCATATTACACA+AGG | - | chr1.4:86510946-86510965 | MS.gene029291:CDS | 35.0% | |
| CACCTAACATTGTCATGATT+TGG | + | chr1.4:86511384-86511403 | None:intergenic | 35.0% | |
| CGAATAAGGTTTGAATGGTT+TGG | + | chr1.4:86511508-86511527 | None:intergenic | 35.0% | |
| CTTGTGTAATATGAACCTGT+TGG | + | chr1.4:86510948-86510967 | None:intergenic | 35.0% | |
| GTATACATAACCATTGGAGA+TGG | - | chr1.4:86511930-86511949 | MS.gene029291:CDS | 35.0% | |
| GTGATGCTTTATAAGGAGTA+TGG | + | chr1.4:86511539-86511558 | None:intergenic | 35.0% | |
| GTTTCTGTCCTGTTAACTTT+AGG | + | chr1.4:86511681-86511700 | None:intergenic | 35.0% | |
| TACTATTATGAGGTTGGACT+TGG | - | chr1.4:86511168-86511187 | MS.gene029291:CDS | 35.0% | |
| TCCCAAATCATGACAATGTT+AGG | - | chr1.4:86511379-86511398 | MS.gene029291:CDS | 35.0% | |
| TCTCCAATGGTTATGTATAC+AGG | + | chr1.4:86511930-86511949 | None:intergenic | 35.0% | |
| TGCATTAGAACAATCAACCA+AGG | + | chr1.4:86511705-86511724 | None:intergenic | 35.0% | |
| TGTGTAATATGAACCTGTTG+GGG | + | chr1.4:86510946-86510965 | None:intergenic | 35.0% | |
| TTACACAAGGTGATCTTGTT+GGG | - | chr1.4:86510959-86510978 | MS.gene029291:CDS | 35.0% | |
| TTGTTCTAATGCATTCACCA+TGG | - | chr1.4:86511712-86511731 | MS.gene029291:CDS | 35.0% | |
| ! | CAACACTGTTTTCCCTTTTT+TGG | + | chr1.4:86511328-86511347 | None:intergenic | 35.0% |
| ! | GGAGCTGATTGATCTTTTAT+AGG | + | chr1.4:86511909-86511928 | None:intergenic | 35.0% |
| ! | TTTTCCATATGCTGAATACG+AGG | + | chr1.4:86511622-86511641 | None:intergenic | 35.0% |
| AATGTTAGGTGGGATACTTG+GGG | - | chr1.4:86511393-86511412 | MS.gene029291:CDS | 40.0% | |
| ACAATGTTAGGTGGGATACT+TGG | - | chr1.4:86511391-86511410 | MS.gene029291:CDS | 40.0% | |
| ACATAACCATTGGAGATGGA+GGG | - | chr1.4:86511934-86511953 | MS.gene029291:CDS | 40.0% | |
| AGGAGCTACCTAAAGTTAAC+AGG | - | chr1.4:86511670-86511689 | MS.gene029291:CDS | 40.0% | |
| CAATGTTAGGTGGGATACTT+GGG | - | chr1.4:86511392-86511411 | MS.gene029291:CDS | 40.0% | |
| CCATACACATTTGGTCTCAT+AGG | - | chr1.4:86511246-86511265 | MS.gene029291:CDS | 40.0% | |
| CCTATGAGACCAAATGTGTA+TGG | + | chr1.4:86511249-86511268 | None:intergenic | 40.0% | |
| GCTCCTGTATACATAACCAT+TGG | - | chr1.4:86511924-86511943 | MS.gene029291:CDS | 40.0% | |
| TACATAACCATTGGAGATGG+AGG | - | chr1.4:86511933-86511952 | MS.gene029291:CDS | 40.0% | |
| TCAAGATGGTTATTCGGTTG+AGG | - | chr1.4:86512088-86512107 | MS.gene029291:CDS | 40.0% | |
| TGAGAGTAATGTATGAGCCA+TGG | - | chr1.4:86511775-86511794 | MS.gene029291:CDS | 40.0% | |
| TGCAATATTCGACACACGTT+CGG | + | chr1.4:86511859-86511878 | None:intergenic | 40.0% | |
| TTAACAGGACAGAAACTCCT+TGG | - | chr1.4:86511685-86511704 | MS.gene029291:CDS | 40.0% | |
| TTCAGCTCACATCATTGTCT+TGG | - | chr1.4:86511596-86511615 | MS.gene029291:CDS | 40.0% | |
| TTTCCTACAGTCCATATCCA+TGG | + | chr1.4:86511450-86511469 | None:intergenic | 40.0% | |
| TTTGGTCTCATAGGAGATCT+TGG | - | chr1.4:86511255-86511274 | MS.gene029291:CDS | 40.0% | |
| ! | ATTGATTTCGCCCCAGAAAT+TGG | - | chr1.4:86511477-86511496 | MS.gene029291:CDS | 40.0% |
| ! | GCACAACTAGTGCTTTTGTT+AGG | - | chr1.4:86510851-86510870 | MS.gene029291:CDS | 40.0% |
| ! | GGAACACAACAAGACAGTTT+TGG | - | chr1.4:86511190-86511209 | MS.gene029291:CDS | 40.0% |
| ! | GTTTGGTTTCGCCAATTTCT+GGG | + | chr1.4:86511491-86511510 | None:intergenic | 40.0% |
| ! | TTGGTGTTTGTGTGTAATGG+AGG | - | chr1.4:86510829-86510848 | MS.gene029291:CDS | 40.0% |
| ! | TTTGGTTTCGCCAATTTCTG+GGG | + | chr1.4:86511490-86511509 | None:intergenic | 40.0% |
| !! | AAGGTTGATGTTGTGTATGC+TGG | - | chr1.4:86511810-86511829 | MS.gene029291:CDS | 40.0% |
| !! | CACAAAGTACTTCACCCTTT+TGG | - | chr1.4:86511556-86511575 | MS.gene029291:CDS | 40.0% |
| !! | GCCATGGTTTGTTAAGTACA+AGG | - | chr1.4:86511791-86511810 | MS.gene029291:CDS | 40.0% |
| !! | GGGAAAACAGTGTTGTTTGT+TGG | - | chr1.4:86511333-86511352 | MS.gene029291:CDS | 40.0% |
| !!! | GATGTTTTTGCTGTTCCTTC+TGG | - | chr1.4:86510910-86510929 | MS.gene029291:CDS | 40.0% |
| AAGGTTTGTAGAAAGGAGCG+TGG | - | chr1.4:86511416-86511435 | MS.gene029291:CDS | 45.0% | |
| ACAGTACCAATGGCTTGAAC+AGG | - | chr1.4:86511650-86511669 | MS.gene029291:CDS | 45.0% | |
| ACATCAGGACCAATTTCAGG+AGG | + | chr1.4:86511225-86511244 | None:intergenic | 45.0% | |
| AGCTGGCACAGAAATCAAGA+TGG | - | chr1.4:86512074-86512093 | MS.gene029291:CDS | 45.0% | |
| CACACGTTCGGTTCTTTCAT+AGG | + | chr1.4:86511847-86511866 | None:intergenic | 45.0% | |
| CCAAATGTGTATGGCACATC+AGG | + | chr1.4:86511240-86511259 | None:intergenic | 45.0% | |
| CCTGATGTGCCATACACATT+TGG | - | chr1.4:86511237-86511256 | MS.gene029291:CDS | 45.0% | |
| CGGTGCGAATAAGGTTTGAA+TGG | + | chr1.4:86511513-86511532 | None:intergenic | 45.0% | |
| CTGGGGCGAAATCAATTTCA+TGG | + | chr1.4:86511473-86511492 | None:intergenic | 45.0% | |
| CTTGGGGAAGGTTTGTAGAA+AGG | - | chr1.4:86511409-86511428 | MS.gene029291:CDS | 45.0% | |
| GAAAGCAGTGATCGTATCGT+GGG | - | chr1.4:86510981-86511000 | MS.gene029291:CDS | 45.0% | |
| GAACACATGCTCACTATAGC+TGG | - | chr1.4:86512057-86512076 | MS.gene029291:CDS | 45.0% | |
| TCAATGGAAAACATGAGCGG+TGG | + | chr1.4:86512162-86512181 | None:intergenic | 45.0% | |
| TCTCTGAATGCTGAGTACTC+TGG | + | chr1.4:86511999-86512018 | None:intergenic | 45.0% | |
| TGGCTCATCAACCATGGATA+TGG | - | chr1.4:86511436-86511455 | MS.gene029291:CDS | 45.0% | |
| TGGTTATAATGCTCCCCAAC+AGG | - | chr1.4:86510930-86510949 | MS.gene029291:CDS | 45.0% | |
| TGTTCAAGCCATTGGTACTG+TGG | + | chr1.4:86511651-86511670 | None:intergenic | 45.0% | |
| TTGGCCTCGTATTCAGCATA+TGG | - | chr1.4:86511615-86511634 | MS.gene029291:CDS | 45.0% | |
| ! | AGAGTACTCAGCATTCAGAG+AGG | - | chr1.4:86511998-86512017 | MS.gene029291:CDS | 45.0% |
| ! | CAACCATGGATATGGACTGT+AGG | - | chr1.4:86511444-86511463 | MS.gene029291:CDS | 45.0% |
| ! | GGTTTGGTTTCGCCAATTTC+TGG | + | chr1.4:86511492-86511511 | None:intergenic | 45.0% |
| !! | GTATGGTATCGGTGCGAATA+AGG | + | chr1.4:86511522-86511541 | None:intergenic | 45.0% |
| !! | TGGGGAGCATTATAACCAGA+AGG | + | chr1.4:86510928-86510947 | None:intergenic | 45.0% |
| !! | TGTTGGTTTCTAAGCCTCCA+AGG | + | chr1.4:86511962-86511981 | None:intergenic | 45.0% |
| AAGGTTCCCTCCATCTCCAA+TGG | + | chr1.4:86511943-86511962 | None:intergenic | 50.0% | |
| AATGCTGAGTACTCTGGCTG+TGG | + | chr1.4:86511993-86512012 | None:intergenic | 50.0% | |
| ATTGGAGATGGAGGGAACCT+TGG | - | chr1.4:86511942-86511961 | MS.gene029291:CDS | 50.0% | |
| GAGCGGTGGAATCATCAACT+GGG | + | chr1.4:86512148-86512167 | None:intergenic | 50.0% | |
| GGAAAGCAGTGATCGTATCG+TGG | - | chr1.4:86510980-86510999 | MS.gene029291:CDS | 50.0% | |
| GGCACATCAGGACCAATTTC+AGG | + | chr1.4:86511228-86511247 | None:intergenic | 50.0% | |
| GTAGCTCCTGTTCAAGCCAT+TGG | + | chr1.4:86511659-86511678 | None:intergenic | 50.0% | |
| TGAGCGGTGGAATCATCAAC+TGG | + | chr1.4:86512149-86512168 | None:intergenic | 50.0% | |
| TTAGGTGGGATACTTGGGGA+AGG | - | chr1.4:86511397-86511416 | MS.gene029291:CDS | 50.0% | |
| ! | TGGCTGTGGTTCTGTCATGT+TGG | + | chr1.4:86511979-86511998 | None:intergenic | 50.0% |
| !! | GCATTCAGAGAGGCTAGCTT+TGG | - | chr1.4:86512008-86512027 | MS.gene029291:CDS | 50.0% |
| GTTCGACCCTGGTTCATCCT+CGG | + | chr1.4:86511010-86511029 | None:intergenic | 55.0% | |
| ! | CGGTTGAGGCTGATTCCCAT+TGG | - | chr1.4:86512102-86512121 | MS.gene029291:CDS | 55.0% |
| GATCGTATCGTGGGTGACCG+AGG | - | chr1.4:86510990-86511009 | MS.gene029291:CDS | 60.0% | |
| GGAGATGGAGGGAACCTTGG+AGG | - | chr1.4:86511945-86511964 | MS.gene029291:CDS | 60.0% | |
| GGAGCGTGGCTCATCAACCA+TGG | - | chr1.4:86511430-86511449 | MS.gene029291:CDS | 60.0% | |
| GGGTGACCGAGGATGAACCA+GGG | - | chr1.4:86511001-86511020 | MS.gene029291:CDS | 60.0% | |
| TGGGTGACCGAGGATGAACC+AGG | - | chr1.4:86511000-86511019 | MS.gene029291:CDS | 60.0% | |
| GGTCGAACGCAGTGCGCTAC+TGG | - | chr1.4:86511022-86511041 | MS.gene029291:CDS | 65.0% | |
| TAGCGCACTGCGTTCGACCC+TGG | + | chr1.4:86511021-86511040 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 86510766 | 86512181 | 86510766 | ID=MS.gene029291 |
| chr1.4 | mRNA | 86510766 | 86512181 | 86510766 | ID=MS.gene029291.t1;Parent=MS.gene029291 |
| chr1.4 | exon | 86510766 | 86512181 | 86510766 | ID=MS.gene029291.t1.exon1;Parent=MS.gene029291.t1 |
| chr1.4 | CDS | 86510766 | 86512181 | 86510766 | ID=cds.MS.gene029291.t1;Parent=MS.gene029291.t1 |
| Gene Sequence |
| Protein sequence |
