AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene029318

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029318.t1 MTR_1g112210 95.026 382 15 1 1 378 1 382 0.0 764
MS.gene029318.t1 MTR_1g112210 92.778 360 13 1 19 378 23 369 0.0 701
MS.gene029318.t1 MTR_1g050448 73.351 379 96 3 1 378 1 375 0.0 602
MS.gene029318.t1 MTR_3g464570 42.216 334 175 5 55 374 43 372 6.99e-99 298
MS.gene029318.t1 MTR_5g071350 40.233 343 181 5 53 374 56 395 1.56e-98 298
MS.gene029318.t1 MTR_2g015720 42.296 331 170 6 55 378 42 358 1.81e-95 289
MS.gene029318.t1 MTR_4g016940 42.075 347 177 8 53 378 84 427 1.76e-92 284
MS.gene029318.t1 MTR_3g049330 42.216 334 169 7 55 378 31 350 4.33e-92 280
MS.gene029318.t1 MTR_2g091175 39.458 332 180 6 54 378 49 366 1.49e-91 280
MS.gene029318.t1 MTR_7g093450 38.947 380 208 8 19 378 212 587 1.39e-89 281
MS.gene029318.t1 MTR_2g073500 42.687 335 167 8 53 378 27 345 3.05e-89 273
MS.gene029318.t1 MTR_0136s0010 41.210 347 184 7 50 378 165 509 4.87e-88 275
MS.gene029318.t1 MTR_2g073510 39.339 333 181 6 51 374 29 349 2.53e-87 268
MS.gene029318.t1 MTR_5g034810 40.719 334 176 7 53 378 33 352 1.21e-86 266
MS.gene029318.t1 MTR_5g033800 36.842 380 216 10 15 378 112 483 4.55e-85 267
MS.gene029318.t1 MTR_7g109550 37.015 335 188 5 55 374 137 463 7.96e-85 265
MS.gene029318.t1 MTR_4g079700 37.725 334 181 8 55 378 47 363 9.07e-85 262
MS.gene029318.t1 MTR_7g068920 40.058 347 184 8 53 378 78 421 1.25e-84 263
MS.gene029318.t1 MTR_5g059400 37.662 385 215 8 17 378 133 515 2.83e-84 266
MS.gene029318.t1 MTR_1g116130 37.662 385 215 8 17 378 133 515 2.83e-84 266
MS.gene029318.t1 MTR_3g070900 40.517 348 171 10 55 374 106 445 8.12e-83 259
MS.gene029318.t1 MTR_8g463790 39.595 346 180 10 55 378 71 409 2.12e-82 258
MS.gene029318.t1 MTR_4g087730 37.234 376 206 10 12 378 8 362 2.47e-82 256
MS.gene029318.t1 MTR_4g088030 35.756 344 201 6 42 378 20 350 6.36e-82 254
MS.gene029318.t1 MTR_4g014440 35.294 391 223 11 10 378 25 407 1.68e-81 255
MS.gene029318.t1 MTR_4g126950 34.896 384 220 8 21 378 67 446 5.42e-81 255
MS.gene029318.t1 MTR_5g082880 40.227 353 172 11 53 374 163 507 8.50e-80 254
MS.gene029318.t1 MTR_3g021090 35.127 353 206 7 30 374 7 344 9.70e-79 248
MS.gene029318.t1 MTR_3g035830 35.211 355 207 7 30 376 7 346 1.48e-78 245
MS.gene029318.t1 MTR_3g071650 35.969 392 222 10 14 378 41 430 4.26e-78 247
MS.gene029318.t1 MTR_1g111010 40.361 332 172 12 53 378 30 341 5.71e-78 244
MS.gene029318.t1 MTR_4g121560 36.413 368 209 8 31 378 91 453 9.70e-78 247
MS.gene029318.t1 MTR_3g021060 34.473 351 197 7 34 376 36 361 4.84e-76 239
MS.gene029318.t1 MTR_5g083260 37.971 345 190 9 55 378 83 424 7.17e-76 241
MS.gene029318.t1 MTR_8g069790 35.988 339 200 7 52 374 175 512 3.44e-75 242
MS.gene029318.t1 MTR_3g464570 41.111 270 142 4 118 374 3 268 6.89e-74 231
MS.gene029318.t1 MTR_7g070060 34.870 347 204 9 54 378 63 409 9.53e-74 235
MS.gene029318.t1 MTR_4g014430 35.244 349 207 7 47 378 100 446 8.83e-73 234
MS.gene029318.t1 MTR_1g058380 34.243 403 231 10 1 378 1 394 2.25e-71 229
MS.gene029318.t1 MTR_7g094750 35.260 346 198 8 55 378 91 432 4.77e-67 219
MS.gene029318.t1 MTR_2g022560 34.232 371 206 9 32 376 77 435 9.39e-67 218
MS.gene029318.t1 MTR_3g090120 35.638 376 204 9 25 378 100 459 2.18e-63 210
MS.gene029318.t1 MTR_8g093530 33.724 341 199 6 53 378 88 416 1.23e-61 204
MS.gene029318.t1 MTR_5g033800 37.262 263 148 6 28 279 124 380 8.82e-58 192
MS.gene029318.t1 MTR_7g070060 37.021 235 139 5 54 279 63 297 6.02e-54 181
MS.gene029318.t1 MTR_7g070060 31.741 293 180 7 106 378 15 307 6.59e-51 172
MS.gene029318.t1 MTR_7g094750 34.454 238 143 3 55 279 91 328 3.44e-48 166
MS.gene029318.t1 MTR_6g061710 29.459 370 215 12 40 374 152 510 2.64e-44 160
MS.gene029318.t1 MTR_1g076690 30.110 362 193 15 55 374 223 566 2.46e-40 150
MS.gene029318.t1 MTR_5g069320 30.466 279 152 6 98 371 145 386 1.88e-39 144
MS.gene029318.t1 MTR_8g027900 27.596 366 224 13 40 374 196 551 6.87e-36 138
MS.gene029318.t1 MTR_8g093870 28.451 355 208 15 53 376 131 470 8.00e-36 136
MS.gene029318.t1 MTR_7g019120 29.745 353 208 12 51 374 75 416 3.04e-35 134
MS.gene029318.t1 MTR_5g035090 27.596 366 212 13 46 374 74 423 4.49e-35 134
MS.gene029318.t1 MTR_7g033230 28.611 360 203 13 53 374 262 605 8.98e-35 135
MS.gene029318.t1 MTR_8g099300 27.020 396 246 14 11 374 31 415 9.16e-35 132
MS.gene029318.t1 MTR_7g033230 28.611 360 203 13 53 374 332 675 2.24e-34 134
MS.gene029318.t1 MTR_3g109840 28.169 355 204 13 55 374 73 411 1.50e-33 129
MS.gene029318.t1 MTR_8g104970 27.869 366 212 15 46 374 111 461 1.84e-33 130
MS.gene029318.t1 MTR_4g092830 27.920 351 214 11 55 375 48 389 5.91e-33 128
MS.gene029318.t1 MTR_6g007777 27.901 362 207 15 53 378 114 457 7.29e-33 128
MS.gene029318.t1 MTR_6g016880 25.735 408 254 12 7 374 17 415 7.82e-33 127
MS.gene029318.t1 MTR_5g035150 26.776 366 209 13 50 374 80 427 3.72e-32 125
MS.gene029318.t1 MTR_2g016060 28.652 356 196 13 59 374 59 396 1.22e-31 124
MS.gene029318.t1 MTR_5g035080 27.451 357 207 13 55 374 75 416 7.64e-31 122
MS.gene029318.t1 MTR_4g125060 27.933 358 212 15 52 378 168 510 9.29e-31 123
MS.gene029318.t1 MTR_4g125060 27.642 369 215 16 45 376 116 469 9.33e-31 122
MS.gene029318.t1 MTR_4g125060 27.933 358 212 15 52 378 197 539 1.18e-30 123
MS.gene029318.t1 MTR_5g035070 29.944 354 203 13 55 374 87 429 1.59e-30 121
MS.gene029318.t1 MTR_6g016875 27.042 355 214 10 53 374 62 404 2.60e-29 117
MS.gene029318.t1 MTR_6g016240 26.764 411 237 16 8 374 22 412 1.38e-25 107
MS.gene029318.t1 MTR_7g019140 46.875 96 50 1 51 145 75 170 1.43e-23 96.7
MS.gene029318.t1 MTR_5g035120 39.216 102 61 1 44 144 64 165 8.82e-20 86.3
MS.gene029318.t1 MTR_3g021150 33.645 107 64 2 240 339 1 107 2.34e-16 75.1
MS.gene029318.t1 MTR_2g022580 35.593 118 64 3 115 220 3 120 1.47e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029318.t1 AT3G54260 61.478 379 132 6 2 376 4 372 1.30e-172 486
MS.gene029318.t1 AT5G58600 40.237 338 185 6 55 378 66 400 1.11e-92 283
MS.gene029318.t1 AT2G31110 38.462 364 198 7 21 374 11 358 6.88e-92 280
MS.gene029318.t1 AT1G29050 41.088 331 175 5 55 378 61 378 1.59e-91 280
MS.gene029318.t1 AT2G30900 40.841 333 174 8 53 374 41 361 1.45e-89 274
MS.gene029318.t1 AT2G34070 39.823 339 181 7 49 378 59 383 1.47e-89 275
MS.gene029318.t1 AT5G01360 38.329 347 191 8 53 378 34 378 5.04e-86 266
MS.gene029318.t1 AT3G62390 38.576 337 187 6 53 374 136 467 2.08e-85 267
MS.gene029318.t1 AT5G01360 38.329 347 191 8 53 378 90 434 2.25e-85 266
MS.gene029318.t1 AT2G30010 40.058 347 179 8 55 378 56 396 3.30e-85 264
MS.gene029318.t1 AT5G06700 37.978 366 202 7 37 378 230 594 4.62e-85 270
MS.gene029318.t1 AT2G30010 40.058 347 179 8 55 378 99 439 5.90e-85 265
MS.gene029318.t1 AT2G42570 38.507 335 180 7 54 378 46 364 1.53e-83 259
MS.gene029318.t1 AT1G78710 38.298 329 182 7 54 374 37 352 1.82e-83 258
MS.gene029318.t1 AT1G73140 38.728 346 187 8 53 378 65 405 3.77e-83 259
MS.gene029318.t1 AT3G12060 39.080 348 190 8 50 378 191 535 8.30e-83 263
MS.gene029318.t1 AT3G55990 37.669 369 200 9 28 374 119 479 5.72e-82 259
MS.gene029318.t1 AT2G40150 38.636 352 180 9 54 374 70 416 2.57e-81 255
MS.gene029318.t1 AT5G20590 38.663 344 190 8 53 378 139 479 5.43e-81 256
MS.gene029318.t1 AT5G49340 37.571 354 199 8 45 378 90 441 3.96e-80 253
MS.gene029318.t1 AT1G60790 36.416 346 199 9 49 374 184 528 2.99e-79 253
MS.gene029318.t1 AT3G14850 37.273 330 184 7 55 374 34 350 4.87e-79 247
MS.gene029318.t1 AT5G01620 37.101 345 195 8 53 378 108 449 1.07e-78 249
MS.gene029318.t1 AT5G01620 37.101 345 195 8 53 378 108 449 1.07e-78 249
MS.gene029318.t1 AT5G01620 37.101 345 195 8 53 378 116 457 1.09e-78 249
MS.gene029318.t1 AT2G40160 37.176 347 195 9 55 378 77 423 3.65e-78 247
MS.gene029318.t1 AT2G40160 36.888 347 196 9 55 378 77 423 4.59e-78 247
MS.gene029318.t1 AT3G11030 37.609 343 195 7 55 378 106 448 1.45e-76 244
MS.gene029318.t1 AT2G38320 32.161 398 243 10 3 378 12 404 1.43e-74 237
MS.gene029318.t1 AT2G40320 34.343 396 235 9 8 378 27 422 9.25e-74 236
MS.gene029318.t1 AT1G48880 35.345 348 180 8 54 378 114 439 1.29e-67 220
MS.gene029318.t1 AT5G19160 34.133 375 209 9 33 376 75 442 1.75e-66 218
MS.gene029318.t1 AT2G31110 38.583 254 153 3 20 272 10 261 1.80e-66 212
MS.gene029318.t1 AT5G58600 41.410 227 130 3 55 279 66 291 3.54e-65 209
MS.gene029318.t1 AT3G62390 41.176 238 129 3 53 279 136 373 4.25e-65 213
MS.gene029318.t1 AT3G06080 35.028 354 195 9 50 376 98 443 8.76e-65 213
MS.gene029318.t1 AT2G38320 33.645 321 193 7 78 378 1 321 1.12e-64 209
MS.gene029318.t1 AT2G30900 42.609 230 127 5 53 279 41 268 1.55e-64 207
MS.gene029318.t1 AT5G06230 34.561 353 197 8 53 378 19 364 1.24e-62 205
MS.gene029318.t1 AT2G34070 38.783 263 139 6 124 378 1 249 2.86e-62 200
MS.gene029318.t1 AT5G06230 34.561 353 197 8 53 378 60 405 3.16e-62 205
MS.gene029318.t1 AT3G11570 32.071 396 230 11 8 378 38 419 5.10e-61 202
MS.gene029318.t1 AT2G40160 39.407 236 132 6 55 279 77 312 1.24e-56 188
MS.gene029318.t1 AT1G78710 31.549 355 176 10 30 378 3 296 9.18e-56 185
MS.gene029318.t1 AT5G20590 38.750 240 134 5 53 279 139 378 6.84e-54 182
MS.gene029318.t1 AT5G19160 37.037 243 140 4 50 279 94 336 1.83e-50 173
MS.gene029318.t1 AT3G14850 35.000 260 147 6 124 374 1 247 8.61e-50 168
MS.gene029318.t1 AT3G06080 35.802 243 143 4 50 279 98 340 1.21e-48 168
MS.gene029318.t1 AT2G31110 39.908 218 106 6 166 374 9 210 2.35e-48 163
MS.gene029318.t1 AT5G06230 34.959 246 141 4 53 279 60 305 1.38e-45 159
MS.gene029318.t1 AT1G70230 30.447 358 190 14 55 378 79 411 1.36e-40 148
MS.gene029318.t1 AT4G25360 29.096 354 211 12 54 374 174 520 2.88e-39 147
MS.gene029318.t1 AT4G25360 29.096 354 211 12 54 374 174 520 2.88e-39 147
MS.gene029318.t1 AT5G51640 28.912 377 223 13 31 374 120 484 1.54e-38 144
MS.gene029318.t1 AT1G01430 26.216 370 237 12 35 374 80 443 3.23e-38 142
MS.gene029318.t1 AT5G64020 27.606 355 219 15 55 376 57 406 4.30e-37 139
MS.gene029318.t1 AT4G01080 28.693 352 211 12 51 374 87 426 5.78e-37 139
MS.gene029318.t1 AT3G28150 28.713 404 215 18 12 376 39 408 1.87e-34 132
MS.gene029318.t1 AT5G20680 28.205 390 221 16 26 378 90 457 2.03e-34 132
MS.gene029318.t1 AT5G20680 28.205 390 221 16 26 378 184 551 4.52e-34 132
MS.gene029318.t1 AT5G20680 28.205 390 221 16 26 378 184 551 4.52e-34 132
MS.gene029318.t1 AT5G20680 28.205 390 221 16 26 378 184 551 4.52e-34 132
MS.gene029318.t1 AT5G20680 28.205 390 221 16 26 378 184 551 4.52e-34 132
MS.gene029318.t1 AT5G20680 28.205 390 221 16 26 378 166 533 5.06e-34 132
MS.gene029318.t1 AT2G37720 28.125 352 217 15 53 378 141 482 9.30e-34 131
MS.gene029318.t1 AT5G15890 27.841 352 214 10 49 374 181 518 2.17e-33 130
MS.gene029318.t1 AT5G15900 31.378 392 202 16 26 378 46 409 2.49e-33 129
MS.gene029318.t1 AT5G15900 30.280 393 205 17 26 378 65 428 3.49e-33 129
MS.gene029318.t1 AT4G11090 28.261 414 244 18 1 374 21 421 2.43e-32 126
MS.gene029318.t1 AT5G64470 26.377 345 222 10 55 375 55 391 2.14e-31 123
MS.gene029318.t1 AT5G64470 26.211 351 221 11 55 375 55 397 5.50e-31 122
MS.gene029318.t1 AT2G14530 26.741 359 214 10 55 378 62 406 6.67e-31 122
MS.gene029318.t1 AT4G23790 27.424 361 209 14 53 374 77 423 1.82e-30 121
MS.gene029318.t1 AT3G02440 28.286 350 209 11 55 374 127 464 2.62e-29 118
MS.gene029318.t1 AT3G02440 28.286 350 209 11 55 374 135 472 2.72e-29 118
MS.gene029318.t1 AT5G64470 26.423 246 162 7 55 283 55 298 3.25e-21 93.6
MS.gene029318.t1 AT5G64470 26.423 246 162 7 55 283 55 298 3.79e-21 93.6

Find 94 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCTGGTGTGCCTGATATT+TGG 0.189016 1.4:-86197050 MS.gene029318:CDS
GTGATTTCTCAGTTGGAAAA+TGG 0.208372 1.4:-86198470 MS.gene029318:CDS
AGACATCAATTGAGTTCTTC+TGG 0.226253 1.4:-86197830 MS.gene029318:CDS
ATTTCTTCCATGAATCTCTT+TGG 0.226552 1.4:+86198493 None:intergenic
GCTCATTGGTGGACTCATTC+TGG 0.278727 1.4:-86197690 MS.gene029318:CDS
TGATTAAATTGTAGGCAAAA+TGG 0.281278 1.4:-86197315 MS.gene029318:intron
AAAGTGGGTGGATTTGAATT+TGG 0.291594 1.4:-86197467 MS.gene029318:CDS
TTTCTTCCATGAATCTCTTT+GGG 0.298282 1.4:+86198494 None:intergenic
AGCATTTCGTTCCAAATATC+AGG 0.327867 1.4:+86197039 None:intergenic
TTCAGCCACATGCATGTTCC+AGG 0.356693 1.4:-86197255 MS.gene029318:CDS
GTAATATCCTGTAGATATAC+TGG 0.367331 1.4:+86197189 None:intergenic
GGACCAGTAGTAGTGCTAAA+AGG 0.371991 1.4:-86197234 MS.gene029318:CDS
TAATAAGAGAATTCTACATT+TGG 0.381121 1.4:-86197772 MS.gene029318:CDS
TTGAAGAGAATGCAAGGTAT+TGG 0.381566 1.4:-86197743 MS.gene029318:CDS
ATAGCATGGAAAGACATTAA+AGG 0.392314 1.4:+86197938 None:intergenic
TTCTTCCAATGCAAGCCAAC+TGG 0.394214 1.4:+86198542 None:intergenic
TGCAGCCATTGGTGCCTCCC+TGG 0.399442 1.4:-86197066 MS.gene029318:CDS
TTTAATGTCTTTCCATGCTA+TGG 0.404785 1.4:-86197936 MS.gene029318:intron
TTGCATTTGATTCAGCTCAT+TGG 0.406976 1.4:-86197704 MS.gene029318:CDS
TATTCCAACTGACAGGAAAA+AGG 0.407152 1.4:-86197975 MS.gene029318:CDS
TTCTTCAGTTCTACCAATAG+TGG 0.407885 1.4:+86197804 None:intergenic
TGTATTGAGTCCTTTCTCAT+AGG 0.414458 1.4:+86197495 None:intergenic
GTTCTTCTGGGCACCACTAT+TGG 0.416633 1.4:-86197817 MS.gene029318:CDS
CATTTGATTCAGCTCATTGG+TGG 0.419213 1.4:-86197701 MS.gene029318:CDS
GACATCAATTGAGTTCTTCT+GGG 0.424420 1.4:-86197829 MS.gene029318:CDS
AGCCAACTGGACACTTCCTC+TGG 0.424797 1.4:+86198555 None:intergenic
AAGAAATGTGATTTCTCAGT+TGG 0.430768 1.4:-86198477 MS.gene029318:CDS
TTTGATGCACTGAAGTATCT+TGG 0.440296 1.4:-86198085 MS.gene029318:CDS
AAATGGAAGTGGAAGCCATT+AGG 0.444002 1.4:-86198348 MS.gene029318:CDS
AGACATTAAAGGACCATCAT+AGG 0.444756 1.4:+86197949 None:intergenic
CATTTCTCATAATCTGAATC+CGG 0.445205 1.4:+86198366 None:intergenic
CTTGGGATGGAACAACCTAA+TGG 0.446475 1.4:+86198333 None:intergenic
AGTCCTCTGATTGCAGCCAT+TGG 0.458504 1.4:-86197077 MS.gene029318:CDS
CGGATTCAGATTATGAGAAA+TGG 0.459664 1.4:-86198365 MS.gene029318:CDS
TTCTATAATGAGAAACCAAT+GGG 0.459846 1.4:-86198026 MS.gene029318:CDS
GTATGGCACCTAGACATAAC+AGG 0.470822 1.4:-86197414 MS.gene029318:intron
AGTGTCCAGTTGGCTTGCAT+TGG 0.472840 1.4:-86198547 MS.gene029318:CDS
TTCCAAATATCAGGCACACC+AGG 0.474779 1.4:+86197048 None:intergenic
TGCAAACCCAAAGAGATTCA+TGG 0.480389 1.4:-86198500 MS.gene029318:CDS
TCTCTTGTATGCTTAGTTCA+AGG 0.493508 1.4:-86198001 MS.gene029318:CDS
CATCACAAACATGAATCAAA+TGG 0.494804 1.4:-86197521 MS.gene029318:CDS
CAAATGGTTGCCTATGAGAA+AGG 0.503172 1.4:-86197505 MS.gene029318:CDS
GAGATTTCCAGTATATCTAC+AGG 0.504762 1.4:-86197196 MS.gene029318:CDS
GCAGTAACTTGTCAAAAGAA+TGG 0.505516 1.4:-86198393 MS.gene029318:CDS
GTTGGAAATGTTACAATCAA+AGG 0.507595 1.4:-86197293 MS.gene029318:CDS
TAAATTGTAGGCAAAATGGT+TGG 0.508760 1.4:-86197311 MS.gene029318:intron
ATGAGAAAGGACTCAATACA+TGG 0.509591 1.4:-86197492 MS.gene029318:CDS
TTTAGCACTACTACTGGTCC+TGG 0.509768 1.4:+86197237 None:intergenic
TATTGGTAGAACTGAAGAAA+GGG 0.514662 1.4:-86197800 MS.gene029318:CDS
AAGGAGTTATTCCAACTGAC+AGG 0.516555 1.4:-86197982 MS.gene029318:CDS
TACCAGGTGGGATTATTACA+TGG 0.524110 1.4:-86197557 MS.gene029318:intron
GCACACCAGGGAGGCACCAA+TGG 0.532334 1.4:+86197061 None:intergenic
GTTACTGCAGTGGCGAGATA+AGG 0.535012 1.4:+86198408 None:intergenic
CAGATTATGAGAAATGGAAG+TGG 0.537613 1.4:-86198359 MS.gene029318:CDS
CTATTGGTAGAACTGAAGAA+AGG 0.537856 1.4:-86197801 MS.gene029318:CDS
ACATCCTTCAGTGTATAGTA+AGG 0.542161 1.4:-86197139 MS.gene029318:CDS
AGCATACAAGAGATTCCCAT+TGG 0.544133 1.4:+86198011 None:intergenic
GACTCAATACATGGGCAAAG+TGG 0.544827 1.4:-86197483 MS.gene029318:CDS
ATGACTGCATTTAGAAGAGA+TGG 0.549263 1.4:-86197162 MS.gene029318:CDS
GAGAGGCAGAAACAAGGTAA+TGG 0.549704 1.4:-86197102 MS.gene029318:CDS
ATTCTATAATGAGAAACCAA+TGG 0.552968 1.4:-86198027 MS.gene029318:CDS
ATTAATATCCTACCATAGCA+TGG 0.553143 1.4:+86197924 None:intergenic
CATTAGGTTGTTCCATCCCA+AGG 0.558505 1.4:-86198332 MS.gene029318:intron
AGGTAATAAGTGAAGAAGAG+AGG 0.559968 1.4:-86197119 MS.gene029318:CDS
GACAGTTGGAATCATAGAGA+GGG 0.562013 1.4:+86198430 None:intergenic
AGTACGTACCTGTTATGTCT+AGG 0.567486 1.4:+86197406 None:intergenic
GCACCAATGGCTGCAATCAG+AGG 0.571338 1.4:+86197074 None:intergenic
GTTTCATCATCACCTTGGGA+TGG 0.572029 1.4:+86198320 None:intergenic
TTGTCAAAAGAATGGACGGC+CGG 0.574038 1.4:-86198385 MS.gene029318:CDS
GGAAAGAGAATGATGTTGGT+GGG 0.575995 1.4:-86198052 MS.gene029318:CDS
ACTCAATACATGGGCAAAGT+GGG 0.582566 1.4:-86197482 MS.gene029318:CDS
GAGAGGAAAGAGAATGATGT+TGG 0.587409 1.4:-86198056 MS.gene029318:CDS
AATGGCTGCAATCAGAGGAC+TGG 0.587580 1.4:+86197079 None:intergenic
GAGAATGCAAGGTATTGGAA+AGG 0.589108 1.4:-86197738 MS.gene029318:CDS
ATCCAGAGGAAGTGTCCAGT+TGG 0.594248 1.4:-86198557 MS.gene029318:CDS
ATTTGATTGAAGAGAATGCA+AGG 0.595081 1.4:-86197749 MS.gene029318:CDS
AAGTATCTTGGTAAAATGAG+AGG 0.596417 1.4:-86198073 MS.gene029318:CDS
AGGTGGGATTATTACATGGA+AGG 0.598017 1.4:-86197553 MS.gene029318:CDS
CAATACATGGGCAAAGTGGG+TGG 0.598037 1.4:-86197479 MS.gene029318:CDS
ATTACCTTACTATACACTGA+AGG 0.598619 1.4:+86197135 None:intergenic
GTTGGAATCATAGAGAGGGT+AGG 0.612157 1.4:+86198434 None:intergenic
TAACTTGTCAAAAGAATGGA+CGG 0.614689 1.4:-86198389 MS.gene029318:CDS
GGAACGAAATGCTGAGTGCA+TGG 0.615044 1.4:-86197029 MS.gene029318:CDS
GAAGAAGAGAGGCAGAAACA+AGG 0.633633 1.4:-86197108 MS.gene029318:CDS
GGACAGTTGGAATCATAGAG+AGG 0.634353 1.4:+86198429 None:intergenic
TCCAAATATCAGGCACACCA+GGG 0.635948 1.4:+86197049 None:intergenic
AGTGGCGAGATAAGGACAGT+TGG 0.638580 1.4:+86198416 None:intergenic
AGGAAAGAGAATGATGTTGG+TGG 0.644337 1.4:-86198053 MS.gene029318:CDS
AGTAGTAGTGCTAAAAGGAG+TGG 0.649261 1.4:-86197229 MS.gene029318:CDS
TGAGAAAGGACTCAATACAT+GGG 0.677756 1.4:-86197491 MS.gene029318:CDS
CTGGTCCTGGAACATGCATG+TGG 0.678164 1.4:+86197250 None:intergenic
ATTATCACTGATCAATCCAG+AGG 0.696073 1.4:-86198571 MS.gene029318:CDS
AAATATCAGGCACACCAGGG+AGG 0.749580 1.4:+86197052 None:intergenic
AGAAACTGAAGATGTCAACA+TGG 0.749769 1.4:-86198523 MS.gene029318:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAATAAAATATTGATGA+AGG + chr1.4:86198290-86198309 None:intergenic 15.0%
!! AGAACATTTGATTAAATTGT+AGG - chr1.4:86198333-86198352 MS.gene029318:CDS 20.0%
!! ATAAAAATGACAGTAAATGA+AGG + chr1.4:86197040-86197059 None:intergenic 20.0%
!! TAATAAGAGAATTCTACATT+TGG - chr1.4:86197884-86197903 MS.gene029318:intron 20.0%
!!! CATTTTTAGAAGGAAAATTA+AGG + chr1.4:86197769-86197788 None:intergenic 20.0%
!!! GTAAGTAAATCATTTTTAGA+AGG + chr1.4:86197779-86197798 None:intergenic 20.0%
!!! TAATCATTTTTACATCCAAA+TGG - chr1.4:86198060-86198079 MS.gene029318:CDS 20.0%
! AAGCATGTAAGAAAACAAAA+AGG + chr1.4:86197386-86197405 None:intergenic 25.0%
! AATCAATCTTGATTAATACC+AGG - chr1.4:86198083-86198102 MS.gene029318:CDS 25.0%
! ATTCTATAATGAGAAACCAA+TGG - chr1.4:86197629-86197648 MS.gene029318:intron 25.0%
! TGATTAAATTGTAGGCAAAA+TGG - chr1.4:86198341-86198360 MS.gene029318:CDS 25.0%
! TTCTATAATGAGAAACCAAT+GGG - chr1.4:86197630-86197649 MS.gene029318:intron 25.0%
!! TAATCAAGATTGATTCCATT+TGG + chr1.4:86198078-86198097 None:intergenic 25.0%
!!! CTGTCATTTTTATTCTTCTT+AGG - chr1.4:86197047-86197066 MS.gene029318:CDS 25.0%
AAGAAATGTGATTTCTCAGT+TGG - chr1.4:86197179-86197198 MS.gene029318:CDS 30.0%
AAGTATCTTGGTAAAATGAG+AGG - chr1.4:86197583-86197602 MS.gene029318:intron 30.0%
AATCTTGATTAATACCAGGT+GGG - chr1.4:86198087-86198106 MS.gene029318:CDS 30.0%
ACATGTTGTTCACTTATTAC+AGG - chr1.4:86197797-86197816 MS.gene029318:CDS 30.0%
AGAAGCTAAATTTATCAGCA+TGG + chr1.4:86197535-86197554 None:intergenic 30.0%
ATAGCATGGAAAGACATTAA+AGG + chr1.4:86197721-86197740 None:intergenic 30.0%
ATTAATATCCTACCATAGCA+TGG + chr1.4:86197735-86197754 None:intergenic 30.0%
ATTACCTTACTATACACTGA+AGG + chr1.4:86198524-86198543 None:intergenic 30.0%
ATTTCTTCCATGAATCTCTT+TGG + chr1.4:86197166-86197185 None:intergenic 30.0%
ATTTGATTGAAGAGAATGCA+AGG - chr1.4:86197907-86197926 MS.gene029318:intron 30.0%
CATCACAAACATGAATCAAA+TGG - chr1.4:86198135-86198154 MS.gene029318:intron 30.0%
CATTTCTCATAATCTGAATC+CGG + chr1.4:86197293-86197312 None:intergenic 30.0%
GTAATATCCTGTAGATATAC+TGG + chr1.4:86198470-86198489 None:intergenic 30.0%
GTTGGAAATGTTACAATCAA+AGG - chr1.4:86198363-86198382 MS.gene029318:CDS 30.0%
TAAATTGTAGGCAAAATGGT+TGG - chr1.4:86198345-86198364 MS.gene029318:CDS 30.0%
TAATGTTTCATCATCACCTT+GGG + chr1.4:86197343-86197362 None:intergenic 30.0%
TATTGGTAGAACTGAAGAAA+GGG - chr1.4:86197856-86197875 MS.gene029318:intron 30.0%
TTAATGTTTCATCATCACCT+TGG + chr1.4:86197344-86197363 None:intergenic 30.0%
TTTAATGTCTTTCCATGCTA+TGG - chr1.4:86197720-86197739 MS.gene029318:CDS 30.0%
TTTCTTCCATGAATCTCTTT+GGG + chr1.4:86197165-86197184 None:intergenic 30.0%
! ATGTGGCTGAAATATTTTAC+AGG + chr1.4:86198392-86198411 None:intergenic 30.0%
! TAACTTGTCAAAAGAATGGA+CGG - chr1.4:86197267-86197286 MS.gene029318:CDS 30.0%
! TGCAACATTTTTGACATGTA+TGG - chr1.4:86197410-86197429 MS.gene029318:intron 30.0%
ACATCCTTCAGTGTATAGTA+AGG - chr1.4:86198517-86198536 MS.gene029318:CDS 35.0%
AGAAACTGAAGATGTCAACA+TGG - chr1.4:86197133-86197152 MS.gene029318:CDS 35.0%
AGACATCAATTGAGTTCTTC+TGG - chr1.4:86197826-86197845 MS.gene029318:CDS 35.0%
AGACATTAAAGGACCATCAT+AGG + chr1.4:86197710-86197729 None:intergenic 35.0%
AGCATTTCGTTCCAAATATC+AGG + chr1.4:86198620-86198639 None:intergenic 35.0%
AGGTAATAAGTGAAGAAGAG+AGG - chr1.4:86198537-86198556 MS.gene029318:CDS 35.0%
ATGACTGCATTTAGAAGAGA+TGG - chr1.4:86198494-86198513 MS.gene029318:CDS 35.0%
ATGAGAAAGGACTCAATACA+TGG - chr1.4:86198164-86198183 MS.gene029318:intron 35.0%
ATTATCACTGATCAATCCAG+AGG - chr1.4:86197085-86197104 MS.gene029318:CDS 35.0%
CAATCTTGATTAATACCAGG+TGG - chr1.4:86198086-86198105 MS.gene029318:CDS 35.0%
CAGATTATGAGAAATGGAAG+TGG - chr1.4:86197297-86197316 MS.gene029318:CDS 35.0%
CGGATTCAGATTATGAGAAA+TGG - chr1.4:86197291-86197310 MS.gene029318:CDS 35.0%
CTATTGGTAGAACTGAAGAA+AGG - chr1.4:86197855-86197874 MS.gene029318:intron 35.0%
GACATCAATTGAGTTCTTCT+GGG - chr1.4:86197827-86197846 MS.gene029318:CDS 35.0%
GAGATTTCCAGTATATCTAC+AGG - chr1.4:86198460-86198479 MS.gene029318:CDS 35.0%
GTGATTTCTCAGTTGGAAAA+TGG - chr1.4:86197186-86197205 MS.gene029318:CDS 35.0%
TATTCCAACTGACAGGAAAA+AGG - chr1.4:86197681-86197700 MS.gene029318:CDS 35.0%
TCTCTTGTATGCTTAGTTCA+AGG - chr1.4:86197655-86197674 MS.gene029318:intron 35.0%
TGAGAAAGGACTCAATACAT+GGG - chr1.4:86198165-86198184 MS.gene029318:intron 35.0%
TGTATTGAGTCCTTTCTCAT+AGG + chr1.4:86198164-86198183 None:intergenic 35.0%
TTCTTCAGTTCTACCAATAG+TGG + chr1.4:86197855-86197874 None:intergenic 35.0%
TTGAAGAGAATGCAAGGTAT+TGG - chr1.4:86197913-86197932 MS.gene029318:intron 35.0%
TTGCATTTGATTCAGCTCAT+TGG - chr1.4:86197952-86197971 MS.gene029318:CDS 35.0%
! AAAGTGGGTGGATTTGAATT+TGG - chr1.4:86198189-86198208 MS.gene029318:intron 35.0%
! CATACTTCTAAAAACGACTC+GGG + chr1.4:86198227-86198246 None:intergenic 35.0%
! CCATACTTCTAAAAACGACT+CGG + chr1.4:86198228-86198247 None:intergenic 35.0%
! GCAGTAACTTGTCAAAAGAA+TGG - chr1.4:86197263-86197282 MS.gene029318:CDS 35.0%
! TTTGATGCACTGAAGTATCT+TGG - chr1.4:86197571-86197590 MS.gene029318:intron 35.0%
AAATGGAAGTGGAAGCCATT+AGG - chr1.4:86197308-86197327 MS.gene029318:intron 40.0%
AAGGAGTTATTCCAACTGAC+AGG - chr1.4:86197674-86197693 MS.gene029318:intron 40.0%
ACTCAATACATGGGCAAAGT+GGG - chr1.4:86198174-86198193 MS.gene029318:intron 40.0%
AGCATACAAGAGATTCCCAT+TGG + chr1.4:86197648-86197667 None:intergenic 40.0%
AGCATGGTTTACGTGTGTTT+GGG + chr1.4:86197519-86197538 None:intergenic 40.0%
AGGAAAAAGGTCACCTATGA+TGG - chr1.4:86197694-86197713 MS.gene029318:CDS 40.0%
AGGAAAGAGAATGATGTTGG+TGG - chr1.4:86197603-86197622 MS.gene029318:intron 40.0%
AGGTGGGATTATTACATGGA+AGG - chr1.4:86198103-86198122 MS.gene029318:intron 40.0%
AGTACGTACCTGTTATGTCT+AGG + chr1.4:86198253-86198272 None:intergenic 40.0%
AGTAGTAGTGCTAAAAGGAG+TGG - chr1.4:86198427-86198446 MS.gene029318:CDS 40.0%
ATGTCTTTCCATGCTATGGT+AGG - chr1.4:86197724-86197743 MS.gene029318:CDS 40.0%
CAAATGGTTGCCTATGAGAA+AGG - chr1.4:86198151-86198170 MS.gene029318:intron 40.0%
CATTTGATTCAGCTCATTGG+TGG - chr1.4:86197955-86197974 MS.gene029318:CDS 40.0%
GACAGTTGGAATCATAGAGA+GGG + chr1.4:86197229-86197248 None:intergenic 40.0%
GAGAGGAAAGAGAATGATGT+TGG - chr1.4:86197600-86197619 MS.gene029318:intron 40.0%
GGAAAGAGAATGATGTTGGT+GGG - chr1.4:86197604-86197623 MS.gene029318:intron 40.0%
TACCAGGTGGGATTATTACA+TGG - chr1.4:86198099-86198118 MS.gene029318:intron 40.0%
TGCAAACCCAAAGAGATTCA+TGG - chr1.4:86197156-86197175 MS.gene029318:CDS 40.0%
TTCCATGTAATAATCCCACC+TGG + chr1.4:86198104-86198123 None:intergenic 40.0%
! ACTCCTTTTAGCACTACTAC+TGG + chr1.4:86198428-86198447 None:intergenic 40.0%
! AGCTTCTCGATGACATTTTC+AGG - chr1.4:86197548-86197567 MS.gene029318:CDS 40.0%
! CTTTTGACAAGTTACTGCAG+TGG + chr1.4:86197261-86197280 None:intergenic 40.0%
!! GAGAATGCAAGGTATTGGAA+AGG - chr1.4:86197918-86197937 MS.gene029318:intron 40.0%
!!! CCGAGTCGTTTTTAGAAGTA+TGG - chr1.4:86198225-86198244 MS.gene029318:intron 40.0%
CAGCATGGTTTACGTGTGTT+TGG + chr1.4:86197520-86197539 None:intergenic 45.0%
CTTGGGATGGAACAACCTAA+TGG + chr1.4:86197326-86197345 None:intergenic 45.0%
GAAGAAGAGAGGCAGAAACA+AGG - chr1.4:86198548-86198567 MS.gene029318:CDS 45.0%
GACTCAATACATGGGCAAAG+TGG - chr1.4:86198173-86198192 MS.gene029318:intron 45.0%
GAGAGGCAGAAACAAGGTAA+TGG - chr1.4:86198554-86198573 MS.gene029318:CDS 45.0%
GGACAGTTGGAATCATAGAG+AGG + chr1.4:86197230-86197249 None:intergenic 45.0%
GGACCAGTAGTAGTGCTAAA+AGG - chr1.4:86198422-86198441 MS.gene029318:CDS 45.0%
GTATGGCACCTAGACATAAC+AGG - chr1.4:86198242-86198261 MS.gene029318:intron 45.0%
GTTGGAATCATAGAGAGGGT+AGG + chr1.4:86197225-86197244 None:intergenic 45.0%
GTTTCATCATCACCTTGGGA+TGG + chr1.4:86197339-86197358 None:intergenic 45.0%
TCCAAATATCAGGCACACCA+GGG + chr1.4:86198610-86198629 None:intergenic 45.0%
TTCCAAATATCAGGCACACC+AGG + chr1.4:86198611-86198630 None:intergenic 45.0%
TTCTTCCAATGCAAGCCAAC+TGG + chr1.4:86197117-86197136 None:intergenic 45.0%
TTGTCAAAAGAATGGACGGC+CGG - chr1.4:86197271-86197290 MS.gene029318:CDS 45.0%
TTTAGCACTACTACTGGTCC+TGG + chr1.4:86198422-86198441 None:intergenic 45.0%
! CATTAGGTTGTTCCATCCCA+AGG - chr1.4:86197324-86197343 MS.gene029318:intron 45.0%
! GTGACCTTTTTCCTGTCAGT+TGG + chr1.4:86197688-86197707 None:intergenic 45.0%
!! AAAACGACTCGGGTTTTTCG+AGG + chr1.4:86198217-86198236 None:intergenic 45.0%
AAATATCAGGCACACCAGGG+AGG + chr1.4:86198607-86198626 None:intergenic 50.0%
AATGGCTGCAATCAGAGGAC+TGG + chr1.4:86198580-86198599 None:intergenic 50.0%
AGTCCTCTGATTGCAGCCAT+TGG - chr1.4:86198579-86198598 MS.gene029318:CDS 50.0%
AGTGGCGAGATAAGGACAGT+TGG + chr1.4:86197243-86197262 None:intergenic 50.0%
CAATACATGGGCAAAGTGGG+TGG - chr1.4:86198177-86198196 MS.gene029318:intron 50.0%
GGAACGAAATGCTGAGTGCA+TGG - chr1.4:86198627-86198646 MS.gene029318:CDS 50.0%
GTTACTGCAGTGGCGAGATA+AGG + chr1.4:86197251-86197270 None:intergenic 50.0%
GTTCTTCTGGGCACCACTAT+TGG - chr1.4:86197839-86197858 MS.gene029318:CDS 50.0%
TCCCTGGTGTGCCTGATATT+TGG - chr1.4:86198606-86198625 MS.gene029318:CDS 50.0%
TTCAGCCACATGCATGTTCC+AGG - chr1.4:86198401-86198420 MS.gene029318:CDS 50.0%
!! AGTGTCCAGTTGGCTTGCAT+TGG - chr1.4:86197109-86197128 MS.gene029318:CDS 50.0%
!! ATCCAGAGGAAGTGTCCAGT+TGG - chr1.4:86197099-86197118 MS.gene029318:CDS 50.0%
!! GCTCATTGGTGGACTCATTC+TGG - chr1.4:86197966-86197985 MS.gene029318:CDS 50.0%
AGCCAACTGGACACTTCCTC+TGG + chr1.4:86197104-86197123 None:intergenic 55.0%
CTGGTCCTGGAACATGCATG+TGG + chr1.4:86198409-86198428 None:intergenic 55.0%
GCACCAATGGCTGCAATCAG+AGG + chr1.4:86198585-86198604 None:intergenic 55.0%
! TGCAGCCATTGGTGCCTCCC+TGG - chr1.4:86198590-86198609 MS.gene029318:CDS 65.0%
!! GCACACCAGGGAGGCACCAA+TGG + chr1.4:86198598-86198617 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 86197023 86198655 86197023 ID=MS.gene029318
chr1.4 mRNA 86197023 86198655 86197023 ID=MS.gene029318.t1;Parent=MS.gene029318
chr1.4 exon 86198333 86198655 86198333 ID=MS.gene029318.t1.exon1;Parent=MS.gene029318.t1
chr1.4 CDS 86198333 86198655 86198333 ID=cds.MS.gene029318.t1;Parent=MS.gene029318.t1
chr1.4 exon 86197937 86198108 86197937 ID=MS.gene029318.t1.exon2;Parent=MS.gene029318.t1
chr1.4 CDS 86197937 86198108 86197937 ID=cds.MS.gene029318.t1;Parent=MS.gene029318.t1
chr1.4 exon 86197678 86197859 86197678 ID=MS.gene029318.t1.exon3;Parent=MS.gene029318.t1
chr1.4 CDS 86197678 86197859 86197678 ID=cds.MS.gene029318.t1;Parent=MS.gene029318.t1
chr1.4 exon 86197415 86197573 86197415 ID=MS.gene029318.t1.exon4;Parent=MS.gene029318.t1
chr1.4 CDS 86197415 86197573 86197415 ID=cds.MS.gene029318.t1;Parent=MS.gene029318.t1
chr1.4 exon 86197023 86197323 86197023 ID=MS.gene029318.t1.exon5;Parent=MS.gene029318.t1
chr1.4 CDS 86197023 86197323 86197023 ID=cds.MS.gene029318.t1;Parent=MS.gene029318.t1
Gene Sequence

>MS.gene029318

ATGGCAAAAACAAACCTTCATTTACTGTCATTTTTATTCTTCTTAGGCTTATTCAACTGCACATTATCACTGATCAATCCAGAGGAAGTGTCCAGTTGGCTTGCATTGGAAGAAACTGAAGATGTCAACATGGTGCAAACCCAAAGAGATTCATGGAAGAAATGTGATTTCTCAGTTGGAAAATGGATATTTGATCAAACCTACCCTCTCTATGATTCCAACTGTCCTTATCTCGCCACTGCAGTAACTTGTCAAAAGAATGGACGGCCGGATTCAGATTATGAGAAATGGAAGTGGAAGCCATTAGGTTGTTCCATCCCAAGGTGATGATGAAACATTAATTGCTCACATTAATTGCTACCTTTTTGTTTTCTTACATGCTTGCATTGCAACATTTTTGACATGTATGGATGTTCTCTACAATTGATTTTTAGTCGACTTTTATGTAAATGTATTCAAACATAAATCACTTTACATTTAACTCACTTTTAGTCCCAAACACACGTAAACCATGCTGATAAATTTAGCTTCTCGATGACATTTTCAGGTTTGATGCACTGAAGTATCTTGGTAAAATGAGAGGAAAGAGAATGATGTTGGTGGGCGATTCTATAATGAGAAACCAATGGGAATCTCTTGTATGCTTAGTTCAAGGAGTTATTCCAACTGACAGGAAAAAGGTCACCTATGATGGTCCTTTAATGTCTTTCCATGCTATGGTAGGATATTAATCAATCTTATATCCTTAATTTTCCTTCTAAAAATGATTTACTTACATGTTGTTCACTTATTACAGGATTTTGAGACATCAATTGAGTTCTTCTGGGCACCACTATTGGTAGAACTGAAGAAAGGGTCTAATAATAAGAGAATTCTACATTTGGATTTGATTGAAGAGAATGCAAGGTATTGGAAAGGAGTTGATATTCTTGCATTTGATTCAGCTCATTGGTGGACTCATTCTGGTCAATCTAGCTCGTAAGAAACATTTCTCATTTTTTAGTTTTGCACTTAATTGAAAGTGTTACATTTGAAGATAATCATTTTTACATCCAAATGGAATCAATCTTGATTAATACCAGGTGGGATTATTACATGGAAGGAAATAGTATCATCACAAACATGAATCAAATGGTTGCCTATGAGAAAGGACTCAATACATGGGCAAAGTGGGTGGATTTGAATTTGGATCCTCGAAAAACCCGAGTCGTTTTTAGAAGTATGGCACCTAGACATAACAGGTACGTACTATCAATTTTTATTTCCTTCATCAATATTTTATTTTTGCTTTAGTCTCAATAACATATTTAAGAACATTTGATTAAATTGTAGGCAAAATGGTTGGAAATGTTACAATCAAAGGCAACCTGTAAAATATTTCAGCCACATGCATGTTCCAGGACCAGTAGTAGTGCTAAAAGGAGTGGTGAAGAGAATGAGATTTCCAGTATATCTACAGGATATTACAACAATGACTGCATTTAGAAGAGATGGACATCCTTCAGTGTATAGTAAGGTAATAAGTGAAGAAGAGAGGCAGAAACAAGGTAATGGCCAGTCCTCTGATTGCAGCCATTGGTGCCTCCCTGGTGTGCCTGATATTTGGAACGAAATGCTGAGTGCATGGCTTTAA
Protein sequence

>MS.gene029318.t1

MAKTNLHLLSFLFFLGLFNCTLSLINPEEVSSWLALEETEDVNMVQTQRDSWKKCDFSVGKWIFDQTYPLYDSNCPYLATAVTCQKNGRPDSDYEKWKWKPLGCSIPRFDALKYLGKMRGKRMMLVGDSIMRNQWESLVCLVQGVIPTDRKKVTYDGPLMSFHAMDFETSIEFFWAPLLVELKKGSNNKRILHLDLIEENARYWKGVDILAFDSAHWWTHSGQSSSWDYYMEGNSIITNMNQMVAYEKGLNTWAKWVDLNLDPRKTRVVFRSMAPRHNRQNGWKCYNQRQPVKYFSHMHVPGPVVVLKGVVKRMRFPVYLQDITTMTAFRRDGHPSVYSKVISEEERQKQGNGQSSDCSHWCLPGVPDIWNEMLSAWL