AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027587

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027587.t1 MTR_5g034810 94.318 352 20 0 3 354 4 355 0.0 695
MS.gene027587.t1 MTR_3g049330 67.135 356 108 5 1 352 1 351 7.94e-175 489
MS.gene027587.t1 MTR_2g073510 53.913 345 150 3 7 348 12 350 8.41e-138 396
MS.gene027587.t1 MTR_2g015720 55.525 362 146 7 1 351 1 358 2.42e-137 395
MS.gene027587.t1 MTR_4g079700 52.368 359 156 4 5 351 8 363 6.46e-133 384
MS.gene027587.t1 MTR_4g088030 52.571 350 159 4 5 352 7 351 1.66e-131 380
MS.gene027587.t1 MTR_4g087730 52.661 357 158 5 4 352 10 363 2.49e-128 372
MS.gene027587.t1 MTR_2g091175 53.894 321 143 3 34 352 50 367 1.26e-125 365
MS.gene027587.t1 MTR_3g021060 50.305 328 158 3 26 351 39 363 9.32e-123 358
MS.gene027587.t1 MTR_3g035830 47.550 347 172 3 7 351 10 348 1.58e-119 349
MS.gene027587.t1 MTR_3g021090 48.688 343 166 4 7 347 10 344 3.35e-119 350
MS.gene027587.t1 MTR_2g073500 49.245 331 147 8 34 354 29 348 2.68e-113 333
MS.gene027587.t1 MTR_5g071350 45.125 359 173 7 17 351 41 399 3.62e-108 322
MS.gene027587.t1 MTR_3g464570 48.955 335 153 7 34 351 43 376 6.34e-107 318
MS.gene027587.t1 MTR_1g111010 46.809 329 155 10 32 354 30 344 1.00e-96 291
MS.gene027587.t1 MTR_1g050448 39.660 353 192 8 12 351 31 375 1.63e-87 268
MS.gene027587.t1 MTR_0136s0010 39.560 364 187 8 13 352 156 510 5.90e-86 269
MS.gene027587.t1 MTR_7g093450 38.889 360 194 7 17 352 231 588 1.51e-85 270
MS.gene027587.t1 MTR_5g033800 39.489 352 186 8 26 353 137 485 2.16e-85 267
MS.gene027587.t1 MTR_4g121560 40.563 355 179 10 25 352 105 454 2.83e-85 266
MS.gene027587.t1 MTR_1g112210 39.521 334 180 7 32 351 57 382 3.70e-84 260
MS.gene027587.t1 MTR_4g126950 39.769 347 181 10 30 352 105 447 3.94e-84 262
MS.gene027587.t1 MTR_7g109550 39.701 335 181 8 34 351 137 467 1.94e-82 258
MS.gene027587.t1 MTR_1g112210 40.120 334 165 10 32 351 57 369 2.67e-82 255
MS.gene027587.t1 MTR_5g059400 38.753 369 199 9 9 352 150 516 8.39e-81 256
MS.gene027587.t1 MTR_1g116130 38.753 369 199 9 9 352 150 516 8.39e-81 256
MS.gene027587.t1 MTR_8g069790 39.650 343 182 6 34 353 178 518 5.05e-79 251
MS.gene027587.t1 MTR_4g016940 38.304 342 187 8 34 351 86 427 3.58e-78 246
MS.gene027587.t1 MTR_3g464570 47.601 271 126 6 96 351 3 272 3.64e-78 241
MS.gene027587.t1 MTR_2g022560 37.861 346 188 7 33 353 96 439 3.41e-76 242
MS.gene027587.t1 MTR_5g082880 38.202 356 179 9 32 352 163 512 7.09e-76 243
MS.gene027587.t1 MTR_3g070900 37.464 347 191 7 32 352 104 450 1.70e-75 240
MS.gene027587.t1 MTR_3g071650 38.192 343 186 8 34 351 89 430 1.15e-73 234
MS.gene027587.t1 MTR_3g090120 38.506 348 184 11 28 353 122 461 2.94e-72 232
MS.gene027587.t1 MTR_8g463790 37.356 348 191 7 31 354 68 412 3.44e-72 230
MS.gene027587.t1 MTR_5g083260 38.040 347 189 9 30 351 79 424 5.81e-71 228
MS.gene027587.t1 MTR_4g014440 34.115 384 216 13 3 349 22 405 2.21e-70 225
MS.gene027587.t1 MTR_1g058380 37.572 346 188 9 32 352 53 395 8.92e-70 224
MS.gene027587.t1 MTR_7g094750 35.143 350 191 8 34 353 91 434 2.48e-69 224
MS.gene027587.t1 MTR_4g014430 35.227 352 203 8 20 348 94 443 7.07e-69 223
MS.gene027587.t1 MTR_7g070060 34.625 387 211 14 4 349 22 407 3.51e-68 220
MS.gene027587.t1 MTR_7g068920 37.427 342 190 8 34 351 80 421 7.92e-68 219
MS.gene027587.t1 MTR_8g093530 33.152 368 221 8 2 351 56 416 1.74e-65 213
MS.gene027587.t1 MTR_5g033800 38.843 242 134 4 26 253 137 378 1.79e-57 191
MS.gene027587.t1 MTR_7g094750 35.021 237 138 4 33 253 90 326 1.35e-47 164
MS.gene027587.t1 MTR_7g070060 33.007 306 177 11 71 349 1 305 1.74e-47 163
MS.gene027587.t1 MTR_7g070060 35.417 240 143 7 27 254 57 296 2.02e-45 158
MS.gene027587.t1 MTR_5g069320 32.982 285 128 9 78 344 147 386 5.19e-43 153
MS.gene027587.t1 MTR_1g076690 28.457 376 225 12 9 347 198 566 1.10e-38 145
MS.gene027587.t1 MTR_5g035150 30.682 352 201 13 32 347 83 427 1.55e-36 137
MS.gene027587.t1 MTR_8g093870 30.919 359 204 15 26 351 125 472 6.29e-36 136
MS.gene027587.t1 MTR_5g035090 30.571 350 198 13 34 347 83 423 1.65e-35 134
MS.gene027587.t1 MTR_6g061710 28.412 359 213 13 27 348 160 511 5.25e-35 134
MS.gene027587.t1 MTR_5g035080 30.086 349 202 11 34 347 75 416 8.66e-35 132
MS.gene027587.t1 MTR_8g027900 28.531 354 218 11 30 352 207 556 5.06e-34 132
MS.gene027587.t1 MTR_7g019120 30.447 358 205 13 28 351 73 420 6.59e-34 130
MS.gene027587.t1 MTR_3g109840 30.857 350 201 13 31 347 70 411 1.07e-33 129
MS.gene027587.t1 MTR_6g016875 31.680 363 204 15 27 353 57 411 1.41e-33 129
MS.gene027587.t1 MTR_6g016240 30.812 357 211 12 27 351 64 416 1.57e-33 129
MS.gene027587.t1 MTR_6g016880 29.383 405 222 16 1 351 25 419 2.73e-33 128
MS.gene027587.t1 MTR_4g125060 29.096 354 215 13 27 351 125 471 3.30e-33 129
MS.gene027587.t1 MTR_4g125060 29.096 354 215 13 27 351 164 510 5.20e-33 129
MS.gene027587.t1 MTR_4g125060 29.096 354 215 13 27 351 193 539 6.77e-33 129
MS.gene027587.t1 MTR_4g092830 28.693 352 212 12 34 352 48 393 9.05e-32 124
MS.gene027587.t1 MTR_7g033230 29.885 348 208 12 32 347 262 605 1.63e-31 125
MS.gene027587.t1 MTR_7g033230 30.000 360 192 13 32 347 332 675 3.96e-31 124
MS.gene027587.t1 MTR_8g104970 28.814 354 216 12 26 347 112 461 1.70e-30 121
MS.gene027587.t1 MTR_5g035070 30.423 355 210 13 26 347 79 429 3.07e-30 120
MS.gene027587.t1 MTR_3g021150 46.552 116 60 1 216 331 1 114 8.23e-30 110
MS.gene027587.t1 MTR_6g007777 27.324 355 224 13 26 351 108 457 3.70e-28 114
MS.gene027587.t1 MTR_8g099300 26.989 352 221 11 34 353 74 421 6.71e-27 110
MS.gene027587.t1 MTR_2g016060 28.291 357 208 16 35 353 56 402 8.21e-27 110
MS.gene027587.t1 MTR_7g019140 44.330 97 52 1 28 122 73 169 1.35e-22 93.6
MS.gene027587.t1 MTR_5g035120 41.758 91 51 1 32 120 73 163 2.04e-18 82.0
MS.gene027587.t1 MTR_3g021080 36.735 98 41 1 255 352 104 180 8.26e-16 75.1
MS.gene027587.t1 MTR_2g022580 34.188 117 63 3 92 194 2 118 1.52e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027587.t1 AT2G30900 55.402 361 152 7 2 354 9 368 2.66e-144 413
MS.gene027587.t1 AT2G42570 55.488 328 140 4 30 354 43 367 3.20e-134 387
MS.gene027587.t1 AT1G78710 54.517 321 143 3 34 352 38 357 1.93e-133 385
MS.gene027587.t1 AT1G29050 55.015 329 142 4 26 351 53 378 3.10e-133 385
MS.gene027587.t1 AT2G34070 53.571 336 149 5 20 351 51 383 7.36e-131 379
MS.gene027587.t1 AT3G14850 55.455 330 140 5 29 353 29 356 3.18e-128 372
MS.gene027587.t1 AT2G31110 53.125 320 146 3 34 351 45 362 5.16e-126 366
MS.gene027587.t1 AT5G58600 48.214 336 157 6 34 352 66 401 3.22e-106 317
MS.gene027587.t1 AT2G30900 56.917 253 103 4 2 248 9 261 4.43e-102 302
MS.gene027587.t1 AT2G30010 44.444 342 167 6 34 352 56 397 4.59e-98 296
MS.gene027587.t1 AT2G30010 44.315 343 168 6 33 352 98 440 1.09e-97 296
MS.gene027587.t1 AT1G78710 45.171 321 120 4 34 352 31 297 2.77e-97 291
MS.gene027587.t1 AT3G14850 55.686 255 108 4 102 353 1 253 5.49e-96 286
MS.gene027587.t1 AT2G34070 54.365 252 110 4 102 351 1 249 3.49e-95 284
MS.gene027587.t1 AT5G20590 41.547 349 169 11 32 352 139 480 3.85e-87 271
MS.gene027587.t1 AT5G06700 40.000 350 182 9 28 352 249 595 4.48e-85 269
MS.gene027587.t1 AT3G62390 40.294 340 179 8 34 353 138 473 8.40e-85 265
MS.gene027587.t1 AT2G31110 52.535 217 101 1 34 248 45 261 8.52e-85 258
MS.gene027587.t1 AT5G49340 40.346 347 175 9 34 352 100 442 1.55e-84 263
MS.gene027587.t1 AT3G54260 40.708 339 171 9 34 354 51 377 1.93e-84 261
MS.gene027587.t1 AT1G60790 39.945 363 187 10 19 353 175 534 4.43e-84 265
MS.gene027587.t1 AT3G12060 39.437 355 187 9 23 352 185 536 9.73e-83 262
MS.gene027587.t1 AT5G19160 39.612 361 178 11 28 354 93 447 2.29e-80 253
MS.gene027587.t1 AT2G31110 55.072 207 91 2 145 351 10 214 4.63e-79 241
MS.gene027587.t1 AT3G06080 38.983 354 187 8 28 354 97 448 5.75e-79 249
MS.gene027587.t1 AT5G01360 37.026 343 191 7 34 351 36 378 9.76e-79 246
MS.gene027587.t1 AT5G01360 37.026 343 191 7 34 351 92 434 4.64e-78 246
MS.gene027587.t1 AT2G40150 37.607 351 187 7 34 352 71 421 3.58e-76 241
MS.gene027587.t1 AT1G48880 36.740 362 204 8 2 347 83 435 4.63e-76 241
MS.gene027587.t1 AT5G58600 50.446 224 107 3 34 253 66 289 7.25e-74 231
MS.gene027587.t1 AT5G06230 37.234 376 203 9 5 352 36 406 8.67e-74 234
MS.gene027587.t1 AT2G38320 35.958 381 208 12 6 352 27 405 1.23e-73 234
MS.gene027587.t1 AT3G55990 36.812 345 192 7 34 352 140 484 2.51e-73 235
MS.gene027587.t1 AT5G06230 37.297 370 199 9 11 352 1 365 2.13e-72 229
MS.gene027587.t1 AT2G40160 37.216 352 184 10 34 352 77 424 7.70e-71 227
MS.gene027587.t1 AT2G40160 37.216 352 184 10 34 352 77 424 1.78e-70 227
MS.gene027587.t1 AT5G01620 35.362 345 197 8 28 348 104 446 1.01e-69 225
MS.gene027587.t1 AT5G01620 35.362 345 197 8 28 348 104 446 1.01e-69 225
MS.gene027587.t1 AT5G01620 35.362 345 197 8 28 348 112 454 1.31e-69 225
MS.gene027587.t1 AT3G11030 38.571 350 180 9 32 351 104 448 1.54e-68 222
MS.gene027587.t1 AT2G40320 37.752 347 190 9 30 351 77 422 1.59e-68 221
MS.gene027587.t1 AT1G73140 34.561 353 204 7 26 354 59 408 1.20e-66 216
MS.gene027587.t1 AT3G11570 36.923 325 181 7 50 352 98 420 6.39e-65 212
MS.gene027587.t1 AT2G38320 35.897 312 175 8 66 352 11 322 7.26e-61 198
MS.gene027587.t1 AT5G19160 40.496 242 128 4 28 253 93 334 1.01e-58 194
MS.gene027587.t1 AT3G62390 41.453 234 123 4 34 253 138 371 1.37e-58 195
MS.gene027587.t1 AT5G20590 41.597 238 123 6 32 253 139 376 1.85e-58 194
MS.gene027587.t1 AT3G06080 39.669 242 130 4 28 253 97 338 3.00e-54 182
MS.gene027587.t1 AT5G06230 36.900 271 146 6 5 253 36 303 3.51e-51 173
MS.gene027587.t1 AT2G40160 39.316 234 128 6 34 253 77 310 9.65e-50 169
MS.gene027587.t1 AT2G37720 31.092 357 194 12 32 351 141 482 5.83e-40 147
MS.gene027587.t1 AT5G64020 30.769 364 194 16 34 351 57 408 7.29e-40 145
MS.gene027587.t1 AT5G64470 30.172 348 211 12 34 354 55 397 9.23e-39 142
MS.gene027587.t1 AT1G70230 30.994 342 203 13 34 351 79 411 7.79e-38 140
MS.gene027587.t1 AT5G64470 29.545 352 214 12 34 354 55 403 1.98e-37 139
MS.gene027587.t1 AT1G01430 28.809 361 214 10 29 352 94 448 3.78e-36 136
MS.gene027587.t1 AT5G20680 28.571 350 216 12 30 351 114 457 6.56e-36 135
MS.gene027587.t1 AT5G51640 30.056 356 198 14 34 347 138 484 1.12e-35 136
MS.gene027587.t1 AT5G20680 28.571 350 216 12 30 351 190 533 1.35e-35 136
MS.gene027587.t1 AT5G20680 28.571 350 216 12 30 351 208 551 1.64e-35 136
MS.gene027587.t1 AT5G20680 28.571 350 216 12 30 351 208 551 1.64e-35 136
MS.gene027587.t1 AT5G20680 28.571 350 216 12 30 351 208 551 1.64e-35 136
MS.gene027587.t1 AT5G20680 28.571 350 216 12 30 351 208 551 1.64e-35 136
MS.gene027587.t1 AT4G25360 26.882 372 212 12 22 347 163 520 5.35e-34 132
MS.gene027587.t1 AT4G25360 26.882 372 212 12 22 347 163 520 5.35e-34 132
MS.gene027587.t1 AT3G28150 29.167 360 198 12 34 353 70 412 1.04e-33 129
MS.gene027587.t1 AT2G14530 28.977 352 209 13 34 351 62 406 8.31e-33 126
MS.gene027587.t1 AT5G15890 28.409 352 226 9 18 347 171 518 1.04e-30 122
MS.gene027587.t1 AT4G11090 28.895 353 212 13 34 352 79 426 2.71e-29 117
MS.gene027587.t1 AT4G01080 27.143 350 215 13 34 352 91 431 5.30e-29 116
MS.gene027587.t1 AT5G15900 28.451 355 215 13 29 351 62 409 9.96e-29 115
MS.gene027587.t1 AT3G02440 27.195 353 211 13 34 351 127 468 1.04e-28 116
MS.gene027587.t1 AT5G15900 28.451 355 215 13 29 351 81 428 1.30e-28 115
MS.gene027587.t1 AT3G02440 27.195 353 211 13 34 351 135 476 1.34e-28 116
MS.gene027587.t1 AT4G23790 27.887 355 215 13 34 352 79 428 4.72e-27 111
MS.gene027587.t1 AT5G64470 29.762 252 153 9 34 264 55 303 6.59e-23 97.8
MS.gene027587.t1 AT5G64470 29.762 252 153 9 34 264 55 303 7.91e-23 97.8

Find 70 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCCTGGATCCAAGTATT+TGG 0.171032 5.1:+25676969 MS.gene027587:CDS
ATTATTGCAGTGGATCAAAA+TGG 0.241073 5.1:+25676905 MS.gene027587:intron
TGGCTTGTTTGTAGATTTAA+TGG 0.267370 5.1:+25675025 MS.gene027587:intron
CAAAAGCAATGCAACAAATA+TGG 0.328912 5.1:-25672535 None:intergenic
TTGCAGATGGGATTTCATTC+AGG 0.342474 5.1:+25676046 MS.gene027587:intron
AAAAGCATATTCCAAGTATC+AGG 0.342861 5.1:-25677168 None:intergenic
GATTTCTTAACAAGACTTAG+AGG 0.343900 5.1:+25675052 MS.gene027587:CDS
TTTCTCTACCTTTACCTTCC+CGG 0.347701 5.1:+25675189 MS.gene027587:CDS
TACTTGAGATAGAGCTTATC+AGG 0.347749 5.1:-25672723 None:intergenic
TTTCCTGGATCCAAGTATTT+GGG 0.360598 5.1:+25676970 MS.gene027587:CDS
TGCAGCCATTGGTGTCTGCC+TGG 0.383241 5.1:+25677141 MS.gene027587:CDS
TGCCTGGTGTTCCTGATACT+TGG 0.385020 5.1:+25677157 MS.gene027587:CDS
ATATGCATCTACATCTTGTA+AGG 0.393113 5.1:+25676933 MS.gene027587:CDS
AGCTCTATCTCAAGTATAGA+TGG 0.407259 5.1:+25672730 MS.gene027587:CDS
ATACCCAGCTGAGTTGGCTT+TGG 0.407507 5.1:+25676999 MS.gene027587:CDS
ACATATTGATATTTAACTCT+TGG 0.407854 5.1:+25675908 MS.gene027587:CDS
AAATACTTGGATCCAGGAAA+TGG 0.408011 5.1:-25676967 None:intergenic
TTCTCCAAAGCCAACTCAGC+TGG 0.409434 5.1:-25677003 None:intergenic
GCTCACCCATCTGTATATAC+TGG 0.409600 5.1:+25677096 MS.gene027587:CDS
GGAAAGAGTCTCTTGTTTGT+TGG 0.412713 5.1:+25675073 MS.gene027587:CDS
TATGGTCAGGTGAGATTCCT+TGG 0.418149 5.1:-25676184 None:intergenic
CCTTGGTAAATTGCATCCAT+TGG 0.438513 5.1:-25672757 None:intergenic
ACTCCTTGAGTTTGAATCAA+TGG 0.440409 5.1:+25675098 MS.gene027587:CDS
TTATTGCAGTGGATCAAAAT+GGG 0.446967 5.1:+25676906 MS.gene027587:intron
GTATGGACCTCCCAAATACT+TGG 0.450171 5.1:-25676980 None:intergenic
ATACAAAGACATGAATCGTT+TGG 0.452117 5.1:+25676082 MS.gene027587:CDS
ACATTGTAAGTGAACGTATT+GGG 0.456349 5.1:+25675833 MS.gene027587:CDS
CTAAAATTAGATTCAATTGA+AGG 0.459132 5.1:+25675862 MS.gene027587:CDS
ACACCAATGGCTGCAGTCAT+TGG 0.459885 5.1:-25677133 None:intergenic
TATTTAACTCTTGGCATTGG+TGG 0.469517 5.1:+25675917 MS.gene027587:CDS
AAAGCCAACTCAGCTGGGTA+TGG 0.480269 5.1:-25676997 None:intergenic
TGATATTTAACTCTTGGCAT+TGG 0.480479 5.1:+25675914 MS.gene027587:CDS
TTACCACCTTATCAAAATTG+AGG 0.481938 5.1:+25677067 MS.gene027587:CDS
AAGAATTCAAATGCAAATAG+AGG 0.486202 5.1:+25672597 MS.gene027587:CDS
GACATTGTAAGTGAACGTAT+TGG 0.489693 5.1:+25675832 MS.gene027587:CDS
CATTCTGAGCTTTCATAAAG+AGG 0.492435 5.1:-25672663 None:intergenic
CTTGGTAAATTGCATCCATT+GGG 0.497452 5.1:-25672756 None:intergenic
CCTCCCAAATACTTGGATCC+AGG 0.505728 5.1:-25676973 None:intergenic
CAATTGAAGGAGGAAGACTA+TGG 0.509859 5.1:+25675875 MS.gene027587:CDS
AGATCTCACCGGGAAGGTAA+AGG 0.512881 5.1:-25675197 None:intergenic
AAAGGTAGAGAAACCTTTGT+TGG 0.517195 5.1:-25675179 None:intergenic
TTGGCATTGGTGGCTTCACA+CGG 0.534349 5.1:+25675927 MS.gene027587:CDS
AGGTCCATACCCAGCTGAGT+TGG 0.548857 5.1:+25676993 MS.gene027587:CDS
CCATGTCCAGTATATACAGA+TGG 0.552564 5.1:-25677102 None:intergenic
GAGAATGGATTCAAGTGTGT+TGG 0.562977 5.1:+25672696 MS.gene027587:CDS
AAAACAAAGGTATTCTTCCA+AGG 0.564139 5.1:+25676167 MS.gene027587:CDS
AAGTATAGATGGCAGCCCAA+TGG 0.570722 5.1:+25672741 MS.gene027587:CDS
TCTCCAAAGCCAACTCAGCT+GGG 0.571289 5.1:-25677002 None:intergenic
ATTCTGAGCTTTCATAAAGA+GGG 0.572418 5.1:-25672662 None:intergenic
ATACCAATGACTGCAGCCAT+TGG 0.577685 5.1:+25677130 MS.gene027587:CDS
TATGCATCTACATCTTGTAA+GGG 0.588457 5.1:+25676934 MS.gene027587:CDS
TTGCCATTGATTCAAACTCA+AGG 0.588655 5.1:-25675101 None:intergenic
GAAGGAGGAAGACTATGGAA+AGG 0.590329 5.1:+25675880 MS.gene027587:CDS
AGATGGGATTTCATTCAGGA+AGG 0.593260 5.1:+25676050 MS.gene027587:intron
CCTGGATCCAAGTATTTGGG+AGG 0.603347 5.1:+25676973 MS.gene027587:CDS
TGGCATTGGTGGCTTCACAC+GGG 0.603454 5.1:+25675928 MS.gene027587:CDS
TACATCTCAGTCACCAACAA+AGG 0.605171 5.1:+25675166 MS.gene027587:CDS
CCATCTGTATATACTGGACA+TGG 0.606025 5.1:+25677102 MS.gene027587:CDS
CATGTCCAGTATATACAGAT+GGG 0.613179 5.1:-25677101 None:intergenic
GTAATATTGAGCAAATGCAC+AGG 0.615565 5.1:-25677048 None:intergenic
TTCCAAGTATCAGGAACACC+AGG 0.616906 5.1:-25677159 None:intergenic
GAACACCAGGCAGACACCAA+TGG 0.622052 5.1:-25677146 None:intergenic
ATGCATCTACATCTTGTAAG+GGG 0.627882 5.1:+25676935 MS.gene027587:CDS
ACTGAGATGTACTTGATATG+TGG 0.635687 5.1:-25675154 None:intergenic
TGTTGATCCTAGCAAAACAA+AGG 0.637400 5.1:+25676154 MS.gene027587:CDS
AAATTAGATTCAATTGAAGG+AGG 0.640627 5.1:+25675865 MS.gene027587:CDS
TGAAGAAGATCTCACCGGGA+AGG 0.646618 5.1:-25675203 None:intergenic
TATTGCAGTGGATCAAAATG+GGG 0.660632 5.1:+25676907 MS.gene027587:intron
TCTGTATATACTGGACATGG+TGG 0.667740 5.1:+25677105 MS.gene027587:CDS
CCAATGGATGCAATTTACCA+AGG 0.689637 5.1:+25672757 MS.gene027587:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTAATTATTTTTTATA+GGG - chr5.1:25673289-25673308 None:intergenic 0.0%
!!! TATTATTAATTATTTTTTAT+AGG - chr5.1:25673290-25673309 None:intergenic 0.0%
!! TATATAACTCTATATAATTT+AGG - chr5.1:25676700-25676719 None:intergenic 10.0%
!! TGATACTAAAAAATAATTAA+AGG - chr5.1:25674812-25674831 None:intergenic 10.0%
!!! GATTAACATAATTAAATTTT+TGG - chr5.1:25674904-25674923 None:intergenic 10.0%
!!! GTTCAATTAATTTTATAATT+TGG - chr5.1:25676835-25676854 None:intergenic 10.0%
!!! TAATCATTTATTTATCTTTT+TGG + chr5.1:25675367-25675386 MS.gene027587:intron 10.0%
!!! TATATTATGAATTTTTTAAG+TGG - chr5.1:25673673-25673692 None:intergenic 10.0%
!!! TTTAAAATATACTTATAAGA+TGG - chr5.1:25676757-25676776 None:intergenic 10.0%
!! AAAAATAATTAAAGGACAAA+AGG - chr5.1:25674804-25674823 None:intergenic 15.0%
!! ACTATAGTACATTTAAATTA+TGG + chr5.1:25675486-25675505 MS.gene027587:intron 15.0%
!! ATAAAACTTACTAAGTATAT+TGG + chr5.1:25676668-25676687 MS.gene027587:intron 15.0%
!! CATTCAAAAAAAGTAAAAAA+AGG + chr5.1:25675634-25675653 MS.gene027587:intron 15.0%
!! TATATGCTAAATAATAGTAA+AGG - chr5.1:25675233-25675252 None:intergenic 15.0%
!! TATGGTATAATAATTTCAAA+TGG + chr5.1:25674972-25674991 MS.gene027587:intron 15.0%
!!! AAAGAATTTTTTTATCACTA+TGG + chr5.1:25674954-25674973 MS.gene027587:intron 15.0%
!!! AAATATGTACTTACTTTTTA+TGG - chr5.1:25676205-25676224 None:intergenic 15.0%
!!! ATTATTTTTTAGTATCACTT+TGG + chr5.1:25674815-25674834 MS.gene027587:intron 15.0%
!!! TCTTTAAAAAATATATATCC+AGG - chr5.1:25676231-25676250 None:intergenic 15.0%
!! AAGGTTACATAAATAAGTAA+GGG - chr5.1:25673817-25673836 None:intergenic 20.0%
!! AATAAAGAGAAATCCAAATA+AGG - chr5.1:25673836-25673855 None:intergenic 20.0%
!! ACATATTGATATTTAACTCT+TGG + chr5.1:25675908-25675927 MS.gene027587:CDS 20.0%
!! ACTTCGTTACATATAAAAAA+GGG + chr5.1:25673138-25673157 MS.gene027587:intron 20.0%
!! ATATAGAGTTATATACAACA+GGG + chr5.1:25676705-25676724 MS.gene027587:intron 20.0%
!! ATATGACATGACAAATTATA+TGG - chr5.1:25674741-25674760 None:intergenic 20.0%
!! CAAATTATAATAGGAATAAG+TGG - chr5.1:25675764-25675783 None:intergenic 20.0%
!! CTAAAATTAGATTCAATTGA+AGG + chr5.1:25675862-25675881 MS.gene027587:CDS 20.0%
!! CTTATTTATGTAACCTTATT+TGG + chr5.1:25673820-25673839 MS.gene027587:intron 20.0%
!! TAAAAAAGAGACAATAGTAA+GGG + chr5.1:25676557-25676576 MS.gene027587:intron 20.0%
!! TAAATAGTAGCAAAGAAAAA+AGG - chr5.1:25672879-25672898 None:intergenic 20.0%
!! TAAGGTTACATAAATAAGTA+AGG - chr5.1:25673818-25673837 None:intergenic 20.0%
!! TAGTTCTTGAAAAAATTGAA+TGG - chr5.1:25675470-25675489 None:intergenic 20.0%
!! TATATAGAGTTATATACAAC+AGG + chr5.1:25676704-25676723 MS.gene027587:intron 20.0%
!! TCACAACAAAATAGTATATT+AGG + chr5.1:25674768-25674787 MS.gene027587:intron 20.0%
!! TCTTTAAATAATTATTGCAG+TGG + chr5.1:25676895-25676914 MS.gene027587:intron 20.0%
!! TGAAGGAAAAAAAAAATACT+TGG - chr5.1:25675738-25675757 None:intergenic 20.0%
!! TTGAAAAATTGAAATGAACT+TGG + chr5.1:25675005-25675024 MS.gene027587:intron 20.0%
!! TTGTTATGTTGAACATATTT+AGG + chr5.1:25673056-25673075 MS.gene027587:intron 20.0%
!!! AAAATTCTTTTTTTTGCACA+AGG - chr5.1:25674944-25674963 None:intergenic 20.0%
!!! AACATAATGTCTTCAAATTT+TGG + chr5.1:25674303-25674322 MS.gene027587:intron 20.0%
!!! AACATTGACTTAAAAATTTG+AGG - chr5.1:25672906-25672925 None:intergenic 20.0%
!!! ACATAATGTCTTCAAATTTT+GGG + chr5.1:25674304-25674323 MS.gene027587:intron 20.0%
!!! ACATTGACTTAAAAATTTGA+GGG - chr5.1:25672905-25672924 None:intergenic 20.0%
!!! ATTCATTTTTCTATCTAACA+TGG - chr5.1:25674252-25674271 None:intergenic 20.0%
!!! CGTTTTAACAACAAATAAAT+AGG + chr5.1:25673693-25673712 MS.gene027587:intron 20.0%
!!! CTAATTAAGCACTTTTTTTA+AGG - chr5.1:25674668-25674687 None:intergenic 20.0%
!!! CTTCAATTGAATCTAATTTT+AGG - chr5.1:25675864-25675883 None:intergenic 20.0%
!!! TAATTTATAATAAAGTGCTC+CGG - chr5.1:25673181-25673200 None:intergenic 20.0%
!!! TACTGTTGATTAAAATTTGA+TGG - chr5.1:25672966-25672985 None:intergenic 20.0%
!!! TTAGAATAAGTTTAAAATCC+AGG - chr5.1:25674569-25674588 None:intergenic 20.0%
!!! TTCATTTTTCTATCTAACAT+GGG - chr5.1:25674251-25674270 None:intergenic 20.0%
!!! TTTATGAAGAATTAAGTTGT+TGG - chr5.1:25676800-25676819 None:intergenic 20.0%
!!! TTTTTAAGTCAATGTTATTG+TGG + chr5.1:25672910-25672929 MS.gene027587:intron 20.0%
!!! TTTTTTTTGAGGAAAAAATG+AGG + chr5.1:25673916-25673935 MS.gene027587:intron 20.0%
! AAACAAGAACACTATTAGAT+TGG + chr5.1:25673432-25673451 MS.gene027587:intron 25.0%
! AAATTAGATTCAATTGAAGG+AGG + chr5.1:25675865-25675884 MS.gene027587:CDS 25.0%
! AAGAATTCAAATGCAAATAG+AGG + chr5.1:25672597-25672616 MS.gene027587:CDS 25.0%
! AATAGTTGTATCAAAGTCAA+TGG + chr5.1:25674070-25674089 MS.gene027587:intron 25.0%
! AATGAATTAGTGAGCATTTA+TGG + chr5.1:25673335-25673354 MS.gene027587:intron 25.0%
! AATTACATGATTCTATAACC+TGG + chr5.1:25674548-25674567 MS.gene027587:intron 25.0%
! ACATAAATAAGTAAGGGAAA+TGG - chr5.1:25673811-25673830 None:intergenic 25.0%
! ATAAATGCTCACTAATTCAT+TGG - chr5.1:25673336-25673355 None:intergenic 25.0%
! ATAGATAGACTTATACTTGA+AGG + chr5.1:25674191-25674210 MS.gene027587:intron 25.0%
! ATGAATTAGTGAGCATTTAT+GGG + chr5.1:25673336-25673355 MS.gene027587:intron 25.0%
! ATGAATTTAAAGGACTCAAA+CGG - chr5.1:25674861-25674880 None:intergenic 25.0%
! ATGAGGTAGTATTATACATA+TGG + chr5.1:25673933-25673952 MS.gene027587:intron 25.0%
! ATGATGTGTAATTCTATTTG+AGG + chr5.1:25675702-25675721 MS.gene027587:intron 25.0%
! ATTAAACATATGTACACACT+AGG - chr5.1:25674709-25674728 None:intergenic 25.0%
! CAACTCTTCTTCATAATTAA+AGG - chr5.1:25674410-25674429 None:intergenic 25.0%
! CACTTCGTTACATATAAAAA+AGG + chr5.1:25673137-25673156 MS.gene027587:intron 25.0%
! CTAGTAACTAATAAACACTA+AGG + chr5.1:25673462-25673481 MS.gene027587:intron 25.0%
! GATAGAAAAATGAATGTTCA+AGG + chr5.1:25674257-25674276 MS.gene027587:intron 25.0%
! GGAAAAAAAAAATACTTGGT+TGG - chr5.1:25675734-25675753 None:intergenic 25.0%
! GTAAAAAAGAGACAATAGTA+AGG + chr5.1:25676556-25676575 MS.gene027587:intron 25.0%
! TGCTAAATAATAGTAAAGGT+AGG - chr5.1:25675229-25675248 None:intergenic 25.0%
! TGGAATTATACTGTTGAAAT+TGG - chr5.1:25676780-25676799 None:intergenic 25.0%
! TGTATTTATCTTATCGTTCA+AGG + chr5.1:25676629-25676648 MS.gene027587:intron 25.0%
! TGTTGATCTTTCACTATATT+TGG - chr5.1:25676435-25676454 None:intergenic 25.0%
! TTCTTCATAATTAAAGGTAG+AGG - chr5.1:25674404-25674423 None:intergenic 25.0%
! TTGAACGATAAGATAAATAC+AGG - chr5.1:25676630-25676649 None:intergenic 25.0%
!! AATTTATAATAAAGTGCTCC+GGG - chr5.1:25673180-25673199 None:intergenic 25.0%
!! AGATGTAATTTTCTATTGAG+AGG - chr5.1:25673627-25673646 None:intergenic 25.0%
!! ATGTATTTTTCTCATCCAAT+TGG + chr5.1:25672815-25672834 MS.gene027587:intron 25.0%
!! CTCTTTTCATTCAAATACAT+GGG + chr5.1:25672559-25672578 MS.gene027587:CDS 25.0%
!! TATAACGGAGATGAATTTAA+AGG - chr5.1:25674871-25674890 None:intergenic 25.0%
!! TCTCTTTTCATTCAAATACA+TGG + chr5.1:25672558-25672577 MS.gene027587:CDS 25.0%
!! TGTATTCTATGTTTTCTAAG+AGG + chr5.1:25673522-25673541 MS.gene027587:intron 25.0%
!! TTTTCTTATACTTTGCAGAT+GGG + chr5.1:25676034-25676053 MS.gene027587:intron 25.0%
!!! ACAAGAAAGCTTTAAAAACA+TGG + chr5.1:25676111-25676130 MS.gene027587:CDS 25.0%
!!! ATCTTTCCTCAATTTTGATA+AGG - chr5.1:25677076-25677095 None:intergenic 25.0%
!!! CAAGAAAGCTTTAAAAACAT+GGG + chr5.1:25676112-25676131 MS.gene027587:CDS 25.0%
!!! GTATTCTATGTTTTCTAAGA+GGG + chr5.1:25673523-25673542 MS.gene027587:intron 25.0%
!!! GTTGAAATAAGTTTTGTGAA+TGG - chr5.1:25673022-25673041 None:intergenic 25.0%
!!! TCAAATTTTGGGTTAAAATG+TGG + chr5.1:25674315-25674334 MS.gene027587:intron 25.0%
!!! TCTCTTTTTTACAATTGAGA+TGG - chr5.1:25676548-25676567 None:intergenic 25.0%
!!! TCTTTTGAAAAAATCGTGTA+TGG + chr5.1:25675580-25675599 MS.gene027587:intron 25.0%
!!! TGATTTGTTTTATGGTAAGT+GGG + chr5.1:25672623-25672642 MS.gene027587:CDS 25.0%
!!! TTTTGGTTTCAAGAGTTTAA+CGG + chr5.1:25675384-25675403 MS.gene027587:intron 25.0%
ACATTGTAAGTGAACGTATT+GGG + chr5.1:25675833-25675852 MS.gene027587:CDS 30.0%
ACTGGTTAAGGATATGAAAA+AGG - chr5.1:25673241-25673260 None:intergenic 30.0%
AGAGATTGTTGTGTATCATA+TGG + chr5.1:25674214-25674233 MS.gene027587:intron 30.0%
ATACAAAGACATGAATCGTT+TGG + chr5.1:25676082-25676101 MS.gene027587:CDS 30.0%
ATATGCATCTACATCTTGTA+AGG + chr5.1:25676933-25676952 MS.gene027587:CDS 30.0%
ATTATTGCAGTGGATCAAAA+TGG + chr5.1:25676905-25676924 MS.gene027587:intron 30.0%
ATTCTGAGCTTTCATAAAGA+GGG - chr5.1:25672665-25672684 None:intergenic 30.0%
CAAAAGCAATGCAACAAATA+TGG - chr5.1:25672538-25672557 None:intergenic 30.0%
CTCTTTCTTTCCCATATAAA+AGG - chr5.1:25673375-25673394 None:intergenic 30.0%
GATACAACTATTGAATCATG+AGG - chr5.1:25674062-25674081 None:intergenic 30.0%
GATTTCTTAACAAGACTTAG+AGG + chr5.1:25675052-25675071 MS.gene027587:CDS 30.0%
GTACTTTCTTGATCTATTCT+TGG - chr5.1:25676291-25676310 None:intergenic 30.0%
TATGCATCTACATCTTGTAA+GGG + chr5.1:25676934-25676953 MS.gene027587:CDS 30.0%
TCTGCAAAGTATAAGAAAAG+AGG - chr5.1:25676033-25676052 None:intergenic 30.0%
TGAATTAGTGAGCATTTATG+GGG + chr5.1:25673337-25673356 MS.gene027587:intron 30.0%
TGATATCCAAGTTACTTGTA+GGG + chr5.1:25673999-25674018 MS.gene027587:intron 30.0%
TGATATTTAACTCTTGGCAT+TGG + chr5.1:25675914-25675933 MS.gene027587:CDS 30.0%
TTACCACCTTATCAAAATTG+AGG + chr5.1:25677067-25677086 MS.gene027587:CDS 30.0%
TTATTGCAGTGGATCAAAAT+GGG + chr5.1:25676906-25676925 MS.gene027587:intron 30.0%
TTCAAGGCAATACAAAACAA+AGG + chr5.1:25676645-25676664 MS.gene027587:intron 30.0%
TTCATCTCCGTTATATAAGT+TGG + chr5.1:25674876-25674895 MS.gene027587:intron 30.0%
TTGAATCCATTCTCCAAAAA+TGG - chr5.1:25672690-25672709 None:intergenic 30.0%
TTTACAATTGAGATGGTTCA+TGG - chr5.1:25676541-25676560 None:intergenic 30.0%
! AAAAGCATATTCCAAGTATC+AGG - chr5.1:25677171-25677190 None:intergenic 30.0%
! ACTTTGATCTCGAACTTTTA+TGG - chr5.1:25676326-25676345 None:intergenic 30.0%
! AGTAAGTACATATTTTCTCC+TGG + chr5.1:25676210-25676229 MS.gene027587:intron 30.0%
! ATTTATAATAAAGTGCTCCG+GGG - chr5.1:25673179-25673198 None:intergenic 30.0%
! CAAAACTGTTGTTGATCTTT+TGG - chr5.1:25673500-25673519 None:intergenic 30.0%
! CAAAAGATCAACAACAGTTT+TGG + chr5.1:25673498-25673517 MS.gene027587:intron 30.0%
! CTTTTCTTATACTTTGCAGA+TGG + chr5.1:25676033-25676052 MS.gene027587:intron 30.0%
! GTAGCTCTGCAAATTATAAT+AGG - chr5.1:25675773-25675792 None:intergenic 30.0%
! TTTGGGACCAACTTATATAA+CGG - chr5.1:25674886-25674905 None:intergenic 30.0%
! TTTTATAGGGAAATGCTAAC+CGG - chr5.1:25673276-25673295 None:intergenic 30.0%
!! AAAACAAAGGTATTCTTCCA+AGG + chr5.1:25676167-25676186 MS.gene027587:CDS 30.0%
!! TGGCTTGTTTGTAGATTTAA+TGG + chr5.1:25675025-25675044 MS.gene027587:intron 30.0%
!! TTTCCTCAATTTTGATAAGG+TGG - chr5.1:25677073-25677092 None:intergenic 30.0%
!!! AAGAATACCTTTGTTTTGCT+AGG - chr5.1:25676164-25676183 None:intergenic 30.0%
!!! AGAGGTTGTGATTTGTTTTA+TGG + chr5.1:25672615-25672634 MS.gene027587:CDS 30.0%
!!! ATGAACTAGATTTTAACTCG+AGG - chr5.1:25676409-25676428 None:intergenic 30.0%
!!! GTGATTTGTTTTATGGTAAG+TGG + chr5.1:25672622-25672641 MS.gene027587:CDS 30.0%
!!! TGAACTAGATTTTAACTCGA+GGG - chr5.1:25676408-25676427 None:intergenic 30.0%
!!! TGTACTTACTTTTTATGGTC+AGG - chr5.1:25676200-25676219 None:intergenic 30.0%
AAAGGTAGAGAAACCTTTGT+TGG - chr5.1:25675182-25675201 None:intergenic 35.0%
AAATACTTGGATCCAGGAAA+TGG - chr5.1:25676970-25676989 None:intergenic 35.0%
AAGCTCATAAATGTAGCGTT+AGG + chr5.1:25673729-25673748 MS.gene027587:intron 35.0%
ACAACTATTGAATCATGAGG+AGG - chr5.1:25674059-25674078 None:intergenic 35.0%
ACACACTTGTATCTCAACAT+AGG - chr5.1:25673778-25673797 None:intergenic 35.0%
ACTCCTTGAGTTTGAATCAA+TGG + chr5.1:25675098-25675117 MS.gene027587:CDS 35.0%
ACTGAGATGTACTTGATATG+TGG - chr5.1:25675157-25675176 None:intergenic 35.0%
AGCTCTATCTCAAGTATAGA+TGG + chr5.1:25672730-25672749 MS.gene027587:CDS 35.0%
AGGGTCATCAATGTTAGAAA+GGG + chr5.1:25676724-25676743 MS.gene027587:intron 35.0%
AGTAGCAAAGAAAAAAGGCA+CGG - chr5.1:25672874-25672893 None:intergenic 35.0%
ATAGGAATAAGTGGTCATGA+AGG - chr5.1:25675755-25675774 None:intergenic 35.0%
ATATCCAAGTTACTTGTAGG+GGG + chr5.1:25674001-25674020 MS.gene027587:intron 35.0%
ATGCATCTACATCTTGTAAG+GGG + chr5.1:25676935-25676954 MS.gene027587:CDS 35.0%
ATTTCCTGGATCCAAGTATT+TGG + chr5.1:25676969-25676988 MS.gene027587:CDS 35.0%
CAACTATTGAATCATGAGGA+GGG - chr5.1:25674058-25674077 None:intergenic 35.0%
CATGTCCAGTATATACAGAT+GGG - chr5.1:25677104-25677123 None:intergenic 35.0%
CATGTGAGGAGTGAAAAATT+AGG - chr5.1:25675969-25675988 None:intergenic 35.0%
CATTCTGAGCTTTCATAAAG+AGG - chr5.1:25672666-25672685 None:intergenic 35.0%
CTTGGTAAATTGCATCCATT+GGG - chr5.1:25672759-25672778 None:intergenic 35.0%
GACATTGTAAGTGAACGTAT+TGG + chr5.1:25675832-25675851 MS.gene027587:CDS 35.0%
GATATCCAAGTTACTTGTAG+GGG + chr5.1:25674000-25674019 MS.gene027587:intron 35.0%
GTAATATTGAGCAAATGCAC+AGG - chr5.1:25677051-25677070 None:intergenic 35.0%
GTGAAAGATCAACAACATTG+AGG + chr5.1:25676441-25676460 MS.gene027587:intron 35.0%
GTGATATCCAAGTTACTTGT+AGG + chr5.1:25673998-25674017 MS.gene027587:intron 35.0%
TACTTGAGATAGAGCTTATC+AGG - chr5.1:25672726-25672745 None:intergenic 35.0%
TATTGCAGTGGATCAAAATG+GGG + chr5.1:25676907-25676926 MS.gene027587:intron 35.0%
TGTCCACCAAAAATGCATTA+CGG - chr5.1:25675816-25675835 None:intergenic 35.0%
TGTTGATCCTAGCAAAACAA+AGG + chr5.1:25676154-25676173 MS.gene027587:CDS 35.0%
TTAAGAAGTGATGTGTACCT+TGG - chr5.1:25672777-25672796 None:intergenic 35.0%
TTTAAAAACATGGGCGAAGT+GGG + chr5.1:25676121-25676140 MS.gene027587:CDS 35.0%
TTTCCTGGATCCAAGTATTT+GGG + chr5.1:25676970-25676989 MS.gene027587:CDS 35.0%
! AAGCTCAGAATGTCCATTTT+TGG + chr5.1:25672674-25672693 MS.gene027587:CDS 35.0%
! GTTTTCCCGTAATGCATTTT+TGG + chr5.1:25675807-25675826 MS.gene027587:CDS 35.0%
! GTTTTGTGCGCCTTTTATAT+GGG + chr5.1:25673362-25673381 MS.gene027587:intron 35.0%
! TATTTAACTCTTGGCATTGG+TGG + chr5.1:25675917-25675936 MS.gene027587:CDS 35.0%
!! AGTTTTGTGCGCCTTTTATA+TGG + chr5.1:25673361-25673380 MS.gene027587:intron 35.0%
!! GAATGTCCATTTTTGGAGAA+TGG + chr5.1:25672681-25672700 MS.gene027587:CDS 35.0%
!! GGATAGACGTTTCTAAAATG+TGG + chr5.1:25673879-25673898 MS.gene027587:intron 35.0%
!! TTGCCATTGATTCAAACTCA+AGG - chr5.1:25675104-25675123 None:intergenic 35.0%
AACCCATAAATTCAACCGCA+AGG - chr5.1:25673102-25673121 None:intergenic 40.0%
ACTTGTATCTCAACATAGGC+AGG - chr5.1:25673774-25673793 None:intergenic 40.0%
AGACCTTGCGGTTGAATTTA+TGG + chr5.1:25673096-25673115 MS.gene027587:intron 40.0%
AGATGGGATTTCATTCAGGA+AGG + chr5.1:25676050-25676069 MS.gene027587:intron 40.0%
AGCATGCATCACTATCCAAT+TGG - chr5.1:25672833-25672852 None:intergenic 40.0%
AGGTCTCAGATTCAAACTCA+AGG - chr5.1:25673082-25673101 None:intergenic 40.0%
ATGAAAAAGCCTCCACTTGA+GGG + chr5.1:25674144-25674163 MS.gene027587:intron 40.0%
ATTGAGAGGAGTAGTAAACG+TGG - chr5.1:25673613-25673632 None:intergenic 40.0%
CAATTGAAGGAGGAAGACTA+TGG + chr5.1:25675875-25675894 MS.gene027587:CDS 40.0%
CAGGGTCATCAATGTTAGAA+AGG + chr5.1:25676723-25676742 MS.gene027587:intron 40.0%
CCAATGGATGCAATTTACCA+AGG + chr5.1:25672757-25672776 MS.gene027587:CDS 40.0%
CCATCTGTATATACTGGACA+TGG + chr5.1:25677102-25677121 MS.gene027587:CDS 40.0%
CCATGTCCAGTATATACAGA+TGG - chr5.1:25677105-25677124 None:intergenic 40.0%
CCTTGGTAAATTGCATCCAT+TGG - chr5.1:25672760-25672779 None:intergenic 40.0%
CGGTTGAATTTATGGGTTTC+AGG + chr5.1:25673104-25673123 MS.gene027587:intron 40.0%
CTGCTTTAATTCTGTCCAAG+AGG - chr5.1:25673410-25673429 None:intergenic 40.0%
GACCTTGCGGTTGAATTTAT+GGG + chr5.1:25673097-25673116 MS.gene027587:intron 40.0%
GATCAACAACATTGAGGAGA+AGG + chr5.1:25676447-25676466 MS.gene027587:intron 40.0%
GCGACAACAATAAGTGTATG+TGG + chr5.1:25674121-25674140 MS.gene027587:intron 40.0%
GTCCACCAAAAATGCATTAC+GGG - chr5.1:25675815-25675834 None:intergenic 40.0%
TAAAAAAGGGTCCTCTTAGC+CGG + chr5.1:25673151-25673170 MS.gene027587:intron 40.0%
TACATCTCAGTCACCAACAA+AGG + chr5.1:25675166-25675185 MS.gene027587:CDS 40.0%
TCGAGATCAAAGTAACGACA+AGG + chr5.1:25676333-25676352 MS.gene027587:intron 40.0%
TCTGTATATACTGGACATGG+TGG + chr5.1:25677105-25677124 MS.gene027587:CDS 40.0%
TGTTAAGAGTAGTGCATGTG+AGG - chr5.1:25675983-25676002 None:intergenic 40.0%
TTGCAGATGGGATTTCATTC+AGG + chr5.1:25676046-25676065 MS.gene027587:intron 40.0%
TTTCTCTACCTTTACCTTCC+CGG + chr5.1:25675189-25675208 MS.gene027587:CDS 40.0%
! AAGGTAGAGGTACACGTTAT+TGG - chr5.1:25674391-25674410 None:intergenic 40.0%
! AGTTTGAATCTGAGACCTTG+CGG + chr5.1:25673084-25673103 MS.gene027587:intron 40.0%
! CTTTAAAAACATGGGCGAAG+TGG + chr5.1:25676120-25676139 MS.gene027587:CDS 40.0%
! GAGAATGGATTCAAGTGTGT+TGG + chr5.1:25672696-25672715 MS.gene027587:CDS 40.0%
! GGAAAGAGTCTCTTGTTTGT+TGG + chr5.1:25675073-25675092 MS.gene027587:CDS 40.0%
! GTAGCAAAGAAAAAAGGCAC+GGG - chr5.1:25672873-25672892 None:intergenic 40.0%
! TAGCAAAGAAAAAAGGCACG+GGG - chr5.1:25672872-25672891 None:intergenic 40.0%
!! TTCCCGTAATGCATTTTTGG+TGG + chr5.1:25675810-25675829 MS.gene027587:CDS 40.0%
AAGTAACGACAAGGAACGAC+AGG + chr5.1:25676342-25676361 MS.gene027587:intron 45.0%
AAGTATAGATGGCAGCCCAA+TGG + chr5.1:25672741-25672760 MS.gene027587:CDS 45.0%
ACACGTTATTGGATCTTGCG+AGG - chr5.1:25674380-25674399 None:intergenic 45.0%
AGATGGTTCATGGTTCCACA+AGG - chr5.1:25676531-25676550 None:intergenic 45.0%
ATACCAATGACTGCAGCCAT+TGG + chr5.1:25677130-25677149 MS.gene027587:CDS 45.0%
CAACAACATTGAGGAGAAGG+AGG + chr5.1:25676450-25676469 MS.gene027587:intron 45.0%
GAAGGAGGAAGACTATGGAA+AGG + chr5.1:25675880-25675899 MS.gene027587:CDS 45.0%
GATGAAAAAGCCTCCACTTG+AGG + chr5.1:25674143-25674162 MS.gene027587:intron 45.0%
GCTCACCCATCTGTATATAC+TGG + chr5.1:25677096-25677115 MS.gene027587:CDS 45.0%
GGGCAAAAACTTCCATTTCC+TGG + chr5.1:25676955-25676974 MS.gene027587:CDS 45.0%
GGGCTAGAGAATCATCTCAT+TGG - chr5.1:25674038-25674057 None:intergenic 45.0%
GTAGGTGAAGAAGATCTCAC+CGG - chr5.1:25675211-25675230 None:intergenic 45.0%
GTATGGACCTCCCAAATACT+TGG - chr5.1:25676983-25677002 None:intergenic 45.0%
TAGGTGAAGAAGATCTCACC+GGG - chr5.1:25675210-25675229 None:intergenic 45.0%
TATGGTCAGGTGAGATTCCT+TGG - chr5.1:25676187-25676206 None:intergenic 45.0%
TGAGTATACTCTCCCTCAAG+TGG - chr5.1:25674159-25674178 None:intergenic 45.0%
TTCCAAGTATCAGGAACACC+AGG - chr5.1:25677162-25677181 None:intergenic 45.0%
!! TACTTGTAGGGGGTTGCTTT+TGG + chr5.1:25674011-25674030 MS.gene027587:intron 45.0%
!!! GAGTTTTGTTGTGCTCCTCT+TGG + chr5.1:25673392-25673411 MS.gene027587:intron 45.0%
!! TTAATAAAAATGAAAAATAA+TGG + chr5.1:25673858-25673877 MS.gene027587:intron 5.0%
!!! ATTAACATAATTAAATTTTT+GGG - chr5.1:25674903-25674922 None:intergenic 5.0%
!!! TATTTTTTTTTTTTTTTTTG+AGG + chr5.1:25673905-25673924 MS.gene027587:intron 5.0%
!!! TTTTTATTTTTTATTTATCT+TGG - chr5.1:25675541-25675560 None:intergenic 5.0%
AAAGCCAACTCAGCTGGGTA+TGG - chr5.1:25677000-25677019 None:intergenic 50.0%
ACACCAATGGCTGCAGTCAT+TGG - chr5.1:25677136-25677155 None:intergenic 50.0%
AGATCTCACCGGGAAGGTAA+AGG - chr5.1:25675200-25675219 None:intergenic 50.0%
ATATCCTTAACCAGTGCCCC+GGG + chr5.1:25673246-25673265 MS.gene027587:intron 50.0%
CATATCCTTAACCAGTGCCC+CGG + chr5.1:25673245-25673264 MS.gene027587:intron 50.0%
CCTCCCAAATACTTGGATCC+AGG - chr5.1:25676976-25676995 None:intergenic 50.0%
CCTGGATCCAAGTATTTGGG+AGG + chr5.1:25676973-25676992 MS.gene027587:CDS 50.0%
GCAACCCCCTACAAGTAACT+TGG - chr5.1:25674008-25674027 None:intergenic 50.0%
GTATACTCTCCCTCAAGTGG+AGG - chr5.1:25674156-25674175 None:intergenic 50.0%
TCTCCAAAGCCAACTCAGCT+GGG - chr5.1:25677005-25677024 None:intergenic 50.0%
TGAAGAAGATCTCACCGGGA+AGG - chr5.1:25675206-25675225 None:intergenic 50.0%
TGCCTGGTGTTCCTGATACT+TGG + chr5.1:25677157-25677176 MS.gene027587:CDS 50.0%
TGGATCTTGCGAGGAGTTCA+TGG - chr5.1:25674371-25674390 None:intergenic 50.0%
TTCTCCAAAGCCAACTCAGC+TGG - chr5.1:25677006-25677025 None:intergenic 50.0%
! ATACCCAGCTGAGTTGGCTT+TGG + chr5.1:25676999-25677018 MS.gene027587:CDS 50.0%
!! TGTAGCGTTAGGCGTCGAAT+CGG + chr5.1:25673740-25673759 MS.gene027587:intron 50.0%
!! TTGGCATTGGTGGCTTCACA+CGG + chr5.1:25675927-25675946 MS.gene027587:CDS 50.0%
AAATGCTAACCGGTGCTCCC+GGG - chr5.1:25673266-25673285 None:intergenic 55.0%
AGGTCCATACCCAGCTGAGT+TGG + chr5.1:25676993-25677012 MS.gene027587:CDS 55.0%
GAAATGCTAACCGGTGCTCC+CGG - chr5.1:25673267-25673286 None:intergenic 55.0%
GAACACCAGGCAGACACCAA+TGG - chr5.1:25677149-25677168 None:intergenic 55.0%
! AATAAAGTGCTCCGGGGCAC+CGG - chr5.1:25673173-25673192 None:intergenic 55.0%
!! GTAGCGTTAGGCGTCGAATC+GGG + chr5.1:25673741-25673760 MS.gene027587:intron 55.0%
!! TGGCATTGGTGGCTTCACAC+GGG + chr5.1:25675928-25675947 MS.gene027587:CDS 55.0%
AATGCTAACCGGTGCTCCCG+GGG - chr5.1:25673265-25673284 None:intergenic 60.0%
! TGCAGCCATTGGTGTCTGCC+TGG + chr5.1:25677141-25677160 MS.gene027587:CDS 60.0%
!! CGTTAGGCGTCGAATCGGGC+GGG + chr5.1:25673745-25673764 MS.gene027587:intron 65.0%
!! GCGTTAGGCGTCGAATCGGG+CGG + chr5.1:25673744-25673763 MS.gene027587:intron 65.0%
!! TGCTCCCGGGGCACTGGTTA+AGG - chr5.1:25673253-25673272 None:intergenic 65.0%
GGTCCTCTTAGCCGGTGCCC+CGG + chr5.1:25673159-25673178 MS.gene027587:intron 70.0%
! AACCAGTGCCCCGGGAGCAC+CGG + chr5.1:25673254-25673273 MS.gene027587:intron 70.0%
AACCGGTGCTCCCGGGGCAC+TGG - chr5.1:25673259-25673278 None:intergenic 75.0%
!! GCTCCGGGGCACCGGCTAAG+AGG - chr5.1:25673165-25673184 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 25672522 25677215 25672522 ID=MS.gene027587
chr5.1 mRNA 25672522 25677215 25672522 ID=MS.gene027587.t1;Parent=MS.gene027587
chr5.1 exon 25672522 25672778 25672522 ID=MS.gene027587.t1.exon1;Parent=MS.gene027587.t1
chr5.1 CDS 25672522 25672778 25672522 ID=cds.MS.gene027587.t1;Parent=MS.gene027587.t1
chr5.1 exon 25675039 25675210 25675039 ID=MS.gene027587.t1.exon2;Parent=MS.gene027587.t1
chr5.1 CDS 25675039 25675210 25675039 ID=cds.MS.gene027587.t1;Parent=MS.gene027587.t1
chr5.1 exon 25675787 25675962 25675787 ID=MS.gene027587.t1.exon3;Parent=MS.gene027587.t1
chr5.1 CDS 25675787 25675962 25675787 ID=cds.MS.gene027587.t1;Parent=MS.gene027587.t1
chr5.1 exon 25676052 25676210 25676052 ID=MS.gene027587.t1.exon4;Parent=MS.gene027587.t1
chr5.1 CDS 25676052 25676210 25676052 ID=cds.MS.gene027587.t1;Parent=MS.gene027587.t1
chr5.1 exon 25676915 25677215 25676915 ID=MS.gene027587.t1.exon5;Parent=MS.gene027587.t1
chr5.1 CDS 25676915 25677215 25676915 ID=cds.MS.gene027587.t1;Parent=MS.gene027587.t1
Gene Sequence

>MS.gene027587

ATGGGGTCTTTTACCATATTTGTTGCATTGCTTTTGTCTCTTTTCATTCAAATACATGGGAGTGATAGTTTTCACAAGAATTCAAATGCAAATAGAGGTTGTGATTTGTTTTATGGTAAGTGGGTGTATGATGATTCATACCCTCTTTATGAAAGCTCAGAATGTCCATTTTTGGAGAATGGATTCAAGTGTGTTGGTCGTCCTGATAAGCTCTATCTCAAGTATAGATGGCAGCCCAATGGATGCAATTTACCAAGGTACACATCACTTCTTAATTAGACATGTCATTTACTATGTATTTTTCTCATCCAATTGGATAGTGATGCATGCTTAAATTTTCTTGTGATCCCCGTGCCTTTTTTCTTTGCTACTATTTATTACCCTCAAATTTTTAAGTCAATGTTATTGTGGCATCATTCTTTTTGATATTATGAACAATTGCCATCAAATTTTAATCAACAGTAGTTTTTATTTTTTATTTTTTATATAATTAACAACCATTCACAAAACTTATTTCAACTGACAATGTTAATATTGTTATGTTGAACATATTTAGGCCTTGAGTTTGAATCTGAGACCTTGCGGTTGAATTTATGGGTTTCAGGTGATATTTGTCACTTCGTTACATATAAAAAAGGGTCCTCTTAGCCGGTGCCCCGGAGCACTTTATTATAAATTAAGTACACAATTTCAATGCATTAACTACTATATAATTTCCTTTTTCATATCCTTAACCAGTGCCCCGGGAGCACCGGTTAGCATTTCCCTATAAAAAATAATTAATAATATAATAATAATTAATAATGAACAACCAATGAATTAGTGAGCATTTATGGGGAAGTTTTGTGCGCCTTTTATATGGGAAAGAAAGAGTTTTGTTGTGCTCCTCTTGGACAGAATTAAAGCAGAAAAACAAGAACACTATTAGATTGGATCTCTACTAGTAACTAATAAACACTAAGGTTGATTAACGAACCAAAAGATCAACAACAGTTTTGGATGTATTCTATGTTTTCTAAGAGGGATGTATATATTATATACTATGATTATCATACTAGAAAAATTTACGCGTGACAATTAATTAGATTCCACGTTTACTACTCCTCTCAATAGAAAATTACATCTTTGAATTGTACTTGTTTTTTAAACCACTTAAAAAATTCATAATATACGTTTTAACAACAAATAAATAGGAATAAAATGAGACAAGCTCATAAATGTAGCGTTAGGCGTCGAATCGGGCGGGAATCCTGCCTATGTTGAGATACAAGTGTGTCATGTGTGAGCCATTTCCCTTACTTATTTATGTAACCTTATTTGGATTTCTCTTTATTAGTTAATAAAAATGAAAAATAATGGATAGACGTTTCTAAAATGTGGTAGTATTTTTTTTTTTTTTTTTGAGGAAAAAATGAGGTAGTATTATACATATGGAGAGACACACAAACTTAATGTCTTAAGATTTTAGTTTAAAATGTGATATCCAAGTTACTTGTAGGGGGTTGCTTTTGGTCCAATGAGATGATTCTCTAGCCCTCCTCATGATTCAATAGTTGTATCAAAGTCAATGGTGATCAAACGACGTGTTTAATTGATGCAGCGACAACAATAAGTGTATGTGGATGAAAAAGCCTCCACTTGAGGGAGAGTATACTCATATATATGTGAAATAGATAGACTTATACTTGAAGGAGAGATTGTTGTGTATCATATGGTAGTTAGAAGACCCATGTTAGATAGAAAAATGAATGTTCAAGGACATGTAAGTAAATAGACTAATAAACATAATGTCTTCAAATTTTGGGTTAAAATGTGGTGTCAAATTCGCTTGTGTGATTGCTATTGACCATGAACTCCTCGCAAGATCCAATAACGTGTACCTCTACCTTTAATTATGAAGAAGAGTTGAATATTTGAACTTATTGCGTCAAAAAAAAATTTGAACTTATTTTATATATTTTTTTACAATAAACTTATTTTATATCTTAAACATTAGTGTTTCGATATACTTGTAAAATAAAAAAAAAATTACATGATTCTATAACCTGGATTTTAAACTTATTCTAATAAATTTTGAAACTATAACTTACAAATAAGTACTTAATTAGATTTTTGTTGTTGAAAATAAATGCTTAATTAGATTCCTTAAAAAAAGTGCTTAATTAGATAAACTTATTAAATAAACCTAGTGTGTACATATGTTTAATTTTGAGACTCCATATAATTTGTCATGTCATATTATCATATCACAACAAAATAGTATATTAGGATAAATTGTTCCTTTTGTCCTTTAATTATTTTTTAGTATCACTTTGGTTTCATAATTAGCTTTTAGTCCGTTTGAGTCCTTTAAATTCATCTCCGTTATATAAGTTGGTCCCAAAAATTTAATTATGTTAATCAAAGAATTTTTTTATCACCTTGTGCAAAAAAAAGAATTTTTTTATCACTATGGTATAATAATTTCAAATGGATACTCAACTTTGAAAAATTGAAATGAACTTGGCTTGTTTGTAGATTTAATGGTGAAGATTTCTTAACAAGACTTAGAGGAAAGAGTCTCTTGTTTGTTGGAGACTCCTTGAGTTTGAATCAATGGCAATCACTCTCTTGCATGATTCACAAATCTATGCCACATATCAAGTACATCTCAGTCACCAACAAAGGTTTCTCTACCTTTACCTTCCCGGTGAGATCTTCTTCACCTACCTTTACTATTATTTAGCATATATTTTTAAAATCATTATTAAGACGATTTTAATTAACAATGTAATTTTTTCTTCAAAAAAAAAAATTAACAATGTAATTTATTTCTAATTGTGTAAGTCTCAAACACTTAGTAACATAATCATTTATTTATCTTTTTGGTTTCAAGAGTTTAACGGCAAAACTAACACACACTTAGAACTTAATGATACAAACATGTTTATCTTTTGTTTTTATCTCCATTCAATTTTTTCAAGAACTATAGTACATTTAAATTATGGTACATTTTAGAGAATAAATCATCAATGTACCAAGATAAATAAAAAATAAAAATTAAATGATGAACTGATTCTCTTTTGAAAAAATCGTGTATGGTCACAAGATTTACACACAATATTTAGACACACATTCAAAAAAAGTAAAAAAAGGTAGAAGAAATAAAAATTAAATAATGCTTATTTCTAGAATAATTAAATGATGTGTAATTCTATTTGAGGTTGTTACCAACCAAGTATTTTTTTTTTCCTTCATGACCACTTATTCCTATTATAATTTGCAGAGCTACAATGTAAAGTTTATGTTTTCCCGTAATGCATTTTTGGTGGACATTGTAAGTGAACGTATTGGGCAAGTCCTAAAATTAGATTCAATTGAAGGAGGAAGACTATGGAAAGGAAATGACATATTGATATTTAACTCTTGGCATTGGTGGCTTCACACGGGAAAAAAACAAATGTACCTAATTTTTCACTCCTCACATGCACTACTCTTAACATTACATGCATAATGCTTGATGCTTATTCCTCTTTTCTTATACTTTGCAGATGGGATTTCATTCAGGAAGGAAAACATATATACAAAGACATGAATCGTTTGGTTATGTACAAGAAAGCTTTAAAAACATGGGCGAAGTGGGTTGATGACAATGTTGATCCTAGCAAAACAAAGGTATTCTTCCAAGGAATCTCACCTGACCATAAAAAGTAAGTACATATTTTCTCCTGGATATATATTTTTTAAAGACAATTTCGTAAAAAATTTAGTCTAAGCACAAGATGCGCCAAGAATAGATCAAGAAAGTACTAAGCGTTATAACCATAAAAGTTCGAGATCAAAGTAACGACAAGGAACGACAGGAAGACAAAGCACATCGCATTATCTTGTTATGTATCATTCACCCTCGAGTTAAAATCTAGTTCATCATCCAAATATAGTGAAAGATCAACAACATTGAGGAGAAGGAGGTAGAAGACTAGAAGTAGTTGATTAAAATGAGAAGTAGAGTAGCATAGCAGCAGCACCTTGTGGAACCATGAACCATCTCAATTGTAAAAAAGAGACAATAGTAAGGGCAACATAAGTAATACATGAACAGAAGATTAATAACTTAAAATGTAATCCTGTATTTATCTTATCGTTCAAGGCAATACAAAACAAAGGATAAAACTTACTAAGTATATTGGAATTTACCTAAATTATATAGAGTTATATACAACAGGGTCATCAATGTTAGAAAGGGTTGAAATCCATCTTATAAGTATATTTTAAACCAATTTCAACAGTATAATTCCAACAACTTAATTCTTCATAAATCTTCTCATTCTCCAAATTATAAAATTAATTGAACAATAAGTTAATGTGCAAGTCTATATATTTGAAATTTACTTTCTTTAAATAATTATTGCAGTGGATCAAAATGGGGTCAATATGCATCTACATCTTGTAAGGGGCAAAAACTTCCATTTCCTGGATCCAAGTATTTGGGAGGTCCATACCCAGCTGAGTTGGCTTTGGAGAAAGTGATAAGAGCTATGTCAAAGCCTGTGCATTTGCTCAATATTACCACCTTATCAAAATTGAGGAAAGATGCTCACCCATCTGTATATACTGGACATGGTGGACATACCAATGACTGCAGCCATTGGTGTCTGCCTGGTGTTCCTGATACTTGGAATATGCTTTTATATGCATCTCTCATTCAAAATTAA
Protein sequence

>MS.gene027587.t1

MGSFTIFVALLLSLFIQIHGSDSFHKNSNANRGCDLFYGKWVYDDSYPLYESSECPFLENGFKCVGRPDKLYLKYRWQPNGCNLPRFNGEDFLTRLRGKSLLFVGDSLSLNQWQSLSCMIHKSMPHIKYISVTNKGFSTFTFPSYNVKFMFSRNAFLVDIVSERIGQVLKLDSIEGGRLWKGNDILIFNSWHWWLHTGKKQIWDFIQEGKHIYKDMNRLVMYKKALKTWAKWVDDNVDPSKTKVFFQGISPDHKNGSKWGQYASTSCKGQKLPFPGSKYLGGPYPAELALEKVIRAMSKPVHLLNITTLSKLRKDAHPSVYTGHGGHTNDCSHWCLPGVPDTWNMLLYASLIQN