AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027586

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027586.t1 MTR_5g034810 94.587 351 19 0 4 354 5 355 0.0 695
MS.gene027586.t1 MTR_3g049330 66.572 353 107 5 3 352 7 351 2.88e-173 486
MS.gene027586.t1 MTR_2g015720 55.525 362 146 7 1 351 1 358 1.88e-139 400
MS.gene027586.t1 MTR_2g073510 56.481 324 138 2 28 348 27 350 4.18e-138 397
MS.gene027586.t1 MTR_4g079700 52.113 355 155 4 9 351 12 363 7.68e-131 379
MS.gene027586.t1 MTR_4g088030 52.478 343 154 4 12 352 16 351 1.46e-130 377
MS.gene027586.t1 MTR_4g087730 52.707 351 153 6 12 352 16 363 6.49e-127 369
MS.gene027586.t1 MTR_2g091175 53.894 321 143 3 34 352 50 367 1.13e-124 363
MS.gene027586.t1 MTR_3g021060 50.313 320 155 2 34 351 46 363 1.18e-121 355
MS.gene027586.t1 MTR_3g021090 50.314 318 154 2 32 347 29 344 6.58e-119 350
MS.gene027586.t1 MTR_3g035830 49.689 322 158 2 32 351 29 348 7.41e-119 347
MS.gene027586.t1 MTR_2g073500 48.943 331 148 8 34 354 29 348 1.77e-112 331
MS.gene027586.t1 MTR_5g071350 46.491 342 159 7 34 351 58 399 3.04e-107 320
MS.gene027586.t1 MTR_3g464570 48.665 337 151 6 34 351 43 376 7.00e-107 318
MS.gene027586.t1 MTR_1g111010 46.084 332 159 10 29 354 27 344 2.50e-96 290
MS.gene027586.t1 MTR_5g033800 39.773 352 185 8 26 353 137 485 9.36e-86 268
MS.gene027586.t1 MTR_1g050448 39.093 353 194 8 12 351 31 375 1.25e-85 263
MS.gene027586.t1 MTR_0136s0010 39.286 364 188 8 13 352 156 510 1.07e-84 266
MS.gene027586.t1 MTR_1g112210 39.820 334 179 7 32 351 57 382 1.46e-84 261
MS.gene027586.t1 MTR_4g121560 41.210 347 172 10 33 352 113 454 3.08e-84 263
MS.gene027586.t1 MTR_7g093450 38.611 360 195 7 17 352 231 588 5.62e-84 266
MS.gene027586.t1 MTR_4g126950 38.764 356 190 10 21 352 96 447 1.22e-83 261
MS.gene027586.t1 MTR_7g109550 39.701 335 181 8 34 351 137 467 1.78e-82 258
MS.gene027586.t1 MTR_1g112210 39.820 334 166 8 32 351 57 369 3.78e-82 254
MS.gene027586.t1 MTR_5g059400 40.870 345 177 9 33 352 174 516 2.24e-80 255
MS.gene027586.t1 MTR_1g116130 40.870 345 177 9 33 352 174 516 2.24e-80 255
MS.gene027586.t1 MTR_8g069790 38.611 360 194 7 19 353 161 518 2.44e-79 252
MS.gene027586.t1 MTR_3g464570 47.619 273 123 5 96 351 3 272 8.16e-79 243
MS.gene027586.t1 MTR_2g022560 38.150 346 187 7 33 353 96 439 1.12e-77 246
MS.gene027586.t1 MTR_4g016940 38.012 342 188 8 34 351 86 427 2.73e-77 244
MS.gene027586.t1 MTR_3g070900 36.286 350 197 7 29 352 101 450 1.12e-74 238
MS.gene027586.t1 MTR_5g082880 38.310 355 180 10 32 352 163 512 3.23e-74 238
MS.gene027586.t1 MTR_3g071650 37.356 348 182 9 34 351 89 430 4.24e-73 233
MS.gene027586.t1 MTR_8g463790 37.356 348 191 7 31 354 68 412 3.59e-72 230
MS.gene027586.t1 MTR_3g090120 37.536 349 189 10 26 353 121 461 4.41e-72 231
MS.gene027586.t1 MTR_5g083260 37.822 349 191 9 28 351 77 424 6.53e-71 227
MS.gene027586.t1 MTR_4g014430 35.227 352 203 8 20 348 94 443 2.29e-70 226
MS.gene027586.t1 MTR_4g014440 34.037 379 220 12 1 349 27 405 4.64e-70 224
MS.gene027586.t1 MTR_7g094750 35.143 350 191 8 34 353 91 434 1.05e-69 225
MS.gene027586.t1 MTR_1g058380 38.040 347 185 11 32 352 53 395 1.75e-69 223
MS.gene027586.t1 MTR_7g070060 35.511 352 197 12 27 349 57 407 9.21e-69 221
MS.gene027586.t1 MTR_7g068920 37.427 342 190 8 34 351 80 421 4.87e-67 217
MS.gene027586.t1 MTR_8g093530 34.006 347 206 7 21 351 77 416 3.88e-64 210
MS.gene027586.t1 MTR_5g033800 39.256 242 133 4 26 253 137 378 5.72e-58 192
MS.gene027586.t1 MTR_7g094750 35.021 237 138 4 33 253 90 326 2.55e-48 166
MS.gene027586.t1 MTR_7g070060 33.793 290 165 10 86 349 17 305 7.61e-47 161
MS.gene027586.t1 MTR_7g070060 35.000 240 144 7 27 254 57 296 3.31e-46 160
MS.gene027586.t1 MTR_5g069320 30.194 361 179 11 12 344 71 386 1.25e-43 155
MS.gene027586.t1 MTR_1g076690 28.877 374 222 12 11 347 200 566 7.83e-39 145
MS.gene027586.t1 MTR_8g093870 31.073 354 200 15 31 351 130 472 1.09e-35 135
MS.gene027586.t1 MTR_8g027900 28.933 356 218 11 28 352 205 556 4.82e-35 135
MS.gene027586.t1 MTR_5g035090 30.447 358 203 14 26 347 76 423 8.65e-35 132
MS.gene027586.t1 MTR_5g035150 30.114 352 203 13 32 347 83 427 2.18e-34 131
MS.gene027586.t1 MTR_5g035080 29.972 357 208 11 26 347 67 416 3.21e-34 130
MS.gene027586.t1 MTR_6g061710 27.966 354 211 13 32 348 165 511 1.43e-33 130
MS.gene027586.t1 MTR_7g019120 30.337 356 204 13 30 351 75 420 1.96e-33 129
MS.gene027586.t1 MTR_3g109840 30.659 349 203 12 31 347 70 411 7.88e-33 127
MS.gene027586.t1 MTR_4g125060 29.096 354 215 13 27 351 125 471 2.41e-32 126
MS.gene027586.t1 MTR_6g016880 30.208 384 219 17 7 351 46 419 2.54e-32 125
MS.gene027586.t1 MTR_4g125060 29.096 354 215 13 27 351 164 510 2.95e-32 126
MS.gene027586.t1 MTR_4g125060 29.096 354 215 13 27 351 193 539 4.62e-32 126
MS.gene027586.t1 MTR_6g016875 31.667 360 208 14 27 353 57 411 4.95e-32 124
MS.gene027586.t1 MTR_4g092830 28.490 351 214 12 34 352 48 393 1.12e-31 124
MS.gene027586.t1 MTR_7g033230 30.172 348 207 12 32 347 262 605 1.29e-30 122
MS.gene027586.t1 MTR_3g021150 46.552 116 60 1 216 331 1 114 1.64e-30 112
MS.gene027586.t1 MTR_7g033230 30.172 348 207 12 32 347 332 675 2.13e-30 122
MS.gene027586.t1 MTR_6g016240 30.114 352 206 12 34 351 71 416 2.35e-30 120
MS.gene027586.t1 MTR_8g104970 29.023 348 211 12 32 347 118 461 2.26e-29 118
MS.gene027586.t1 MTR_5g035070 30.141 355 211 13 26 347 79 429 4.04e-29 117
MS.gene027586.t1 MTR_6g007777 27.794 349 218 13 32 351 114 457 1.69e-28 115
MS.gene027586.t1 MTR_8g099300 26.705 352 222 11 34 353 74 421 4.32e-26 108
MS.gene027586.t1 MTR_2g016060 28.011 357 209 16 35 353 56 402 8.99e-26 107
MS.gene027586.t1 MTR_7g019140 42.268 97 54 1 28 122 73 169 4.20e-21 89.4
MS.gene027586.t1 MTR_5g035120 40.659 91 52 1 32 120 73 163 2.89e-17 79.0
MS.gene027586.t1 MTR_3g021080 36.735 98 41 1 255 352 104 180 1.64e-16 77.0
MS.gene027586.t1 MTR_2g022580 34.188 117 63 3 92 194 2 118 1.12e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027586.t1 AT2G30900 55.125 361 153 8 2 354 9 368 7.69e-143 409
MS.gene027586.t1 AT2G42570 54.518 332 145 4 26 354 39 367 1.21e-133 386
MS.gene027586.t1 AT1G29050 52.958 355 159 5 2 351 27 378 2.21e-133 385
MS.gene027586.t1 AT1G78710 54.489 323 140 5 34 352 38 357 2.07e-132 382
MS.gene027586.t1 AT2G34070 54.167 336 147 5 20 351 51 383 2.44e-130 378
MS.gene027586.t1 AT3G14850 55.623 329 139 5 30 353 30 356 4.22e-128 371
MS.gene027586.t1 AT2G31110 52.812 320 147 3 34 351 45 362 8.72e-126 365
MS.gene027586.t1 AT5G58600 47.619 336 159 6 34 352 66 401 2.35e-105 315
MS.gene027586.t1 AT2G30900 56.522 253 104 5 2 248 9 261 4.09e-101 300
MS.gene027586.t1 AT2G30010 43.804 347 172 6 29 352 51 397 6.09e-98 296
MS.gene027586.t1 AT2G30010 43.804 347 172 6 29 352 94 440 1.22e-97 296
MS.gene027586.t1 AT1G78710 45.062 324 118 6 34 353 31 298 1.82e-96 289
MS.gene027586.t1 AT3G14850 56.078 255 107 4 102 353 1 253 2.56e-96 286
MS.gene027586.t1 AT2G34070 54.762 252 109 4 102 351 1 249 9.14e-96 285
MS.gene027586.t1 AT5G20590 41.261 349 170 11 32 352 139 480 2.91e-86 269
MS.gene027586.t1 AT3G54260 40.708 339 171 9 34 354 51 377 2.23e-85 263
MS.gene027586.t1 AT2G31110 52.074 217 102 1 34 248 45 261 1.34e-84 257
MS.gene027586.t1 AT5G49340 40.634 347 174 9 34 352 100 442 1.81e-84 263
MS.gene027586.t1 AT3G62390 40.000 340 180 8 34 353 138 473 1.92e-84 264
MS.gene027586.t1 AT5G06700 39.884 346 180 9 32 352 253 595 2.07e-83 265
MS.gene027586.t1 AT1G60790 39.945 363 187 10 19 353 175 534 3.73e-83 262
MS.gene027586.t1 AT3G12060 39.595 346 181 9 32 352 194 536 4.85e-81 257
MS.gene027586.t1 AT5G19160 40.169 356 173 11 33 354 98 447 2.06e-80 253
MS.gene027586.t1 AT2G31110 55.072 207 91 2 145 351 10 214 4.38e-79 241
MS.gene027586.t1 AT3G06080 39.255 349 183 8 33 354 102 448 4.95e-79 249
MS.gene027586.t1 AT5G01360 36.735 343 192 7 34 351 36 378 1.56e-77 243
MS.gene027586.t1 AT5G01360 36.735 343 192 7 34 351 92 434 6.52e-77 243
MS.gene027586.t1 AT1G48880 37.249 349 194 8 15 347 96 435 1.73e-75 240
MS.gene027586.t1 AT2G40150 37.322 351 188 7 34 352 71 421 2.82e-75 239
MS.gene027586.t1 AT2G38320 35.279 394 210 12 2 352 14 405 6.97e-75 237
MS.gene027586.t1 AT5G58600 50.000 224 108 3 34 253 66 289 1.70e-73 229
MS.gene027586.t1 AT3G55990 36.522 345 193 7 34 352 140 484 1.04e-72 234
MS.gene027586.t1 AT5G06230 37.607 351 189 8 30 352 17 365 5.38e-72 228
MS.gene027586.t1 AT5G06230 36.702 376 205 9 5 352 36 406 6.22e-72 229
MS.gene027586.t1 AT2G40160 36.932 352 185 10 34 352 77 424 1.63e-70 226
MS.gene027586.t1 AT2G40160 36.932 352 185 10 34 352 77 424 4.18e-70 226
MS.gene027586.t1 AT5G01620 35.673 342 194 8 31 348 107 446 6.02e-70 226
MS.gene027586.t1 AT5G01620 35.673 342 194 8 31 348 107 446 6.02e-70 226
MS.gene027586.t1 AT5G01620 35.673 342 194 8 31 348 115 454 6.28e-70 226
MS.gene027586.t1 AT2G40320 37.536 349 192 9 28 351 75 422 1.17e-68 221
MS.gene027586.t1 AT3G11030 38.136 354 184 9 28 351 100 448 2.50e-68 221
MS.gene027586.t1 AT1G73140 34.278 353 205 7 26 354 59 408 2.40e-66 215
MS.gene027586.t1 AT3G11570 36.615 325 182 7 50 352 98 420 3.10e-64 210
MS.gene027586.t1 AT2G38320 35.577 312 176 8 66 352 11 322 2.49e-60 197
MS.gene027586.t1 AT5G19160 41.350 237 123 4 33 253 98 334 6.62e-59 194
MS.gene027586.t1 AT3G62390 41.026 234 124 4 34 253 138 371 3.35e-58 194
MS.gene027586.t1 AT5G20590 41.176 238 124 6 32 253 139 376 3.87e-58 193
MS.gene027586.t1 AT3G06080 40.506 237 125 4 33 253 102 338 6.40e-55 184
MS.gene027586.t1 AT2G40160 38.889 234 129 6 34 253 77 310 1.25e-49 169
MS.gene027586.t1 AT5G06230 36.162 271 148 6 5 253 36 303 2.07e-49 168
MS.gene027586.t1 AT2G37720 31.111 360 196 12 29 351 138 482 1.11e-39 146
MS.gene027586.t1 AT5G64020 30.769 364 194 16 34 351 57 408 2.14e-39 144
MS.gene027586.t1 AT5G64470 30.172 348 211 12 34 354 55 397 3.19e-38 141
MS.gene027586.t1 AT1G70230 30.994 342 203 13 34 351 79 411 2.15e-37 139
MS.gene027586.t1 AT5G20680 28.412 359 223 12 21 351 105 457 5.22e-37 139
MS.gene027586.t1 AT5G64470 29.545 352 214 12 34 354 55 403 5.32e-37 138
MS.gene027586.t1 AT5G20680 28.412 359 223 12 21 351 181 533 1.20e-36 139
MS.gene027586.t1 AT5G20680 28.412 359 223 12 21 351 199 551 2.01e-36 139
MS.gene027586.t1 AT5G20680 28.412 359 223 12 21 351 199 551 2.01e-36 139
MS.gene027586.t1 AT5G20680 28.412 359 223 12 21 351 199 551 2.01e-36 139
MS.gene027586.t1 AT5G20680 28.412 359 223 12 21 351 199 551 2.01e-36 139
MS.gene027586.t1 AT1G01430 28.933 356 210 10 34 352 99 448 5.55e-35 133
MS.gene027586.t1 AT5G51640 29.494 356 200 14 34 347 138 484 7.28e-35 134
MS.gene027586.t1 AT3G28150 29.167 360 198 12 34 353 70 412 6.85e-33 127
MS.gene027586.t1 AT4G25360 26.575 365 222 11 22 347 163 520 2.87e-32 127
MS.gene027586.t1 AT4G25360 26.575 365 222 11 22 347 163 520 2.87e-32 127
MS.gene027586.t1 AT2G14530 27.920 351 214 13 34 351 62 406 3.65e-31 122
MS.gene027586.t1 AT5G15890 29.464 336 211 10 34 347 187 518 2.71e-30 121
MS.gene027586.t1 AT4G11090 28.933 356 218 12 29 352 74 426 7.11e-29 116
MS.gene027586.t1 AT4G01080 26.685 356 221 13 28 352 85 431 7.65e-29 116
MS.gene027586.t1 AT5G15900 28.177 362 215 14 26 351 57 409 2.02e-28 115
MS.gene027586.t1 AT5G15900 28.177 362 215 14 26 351 76 428 2.49e-28 115
MS.gene027586.t1 AT3G02440 26.912 353 212 14 34 351 127 468 1.10e-27 113
MS.gene027586.t1 AT3G02440 26.912 353 212 14 34 351 135 476 1.22e-27 113
MS.gene027586.t1 AT4G23790 27.222 360 221 12 29 352 74 428 3.42e-26 108
MS.gene027586.t1 AT5G64470 29.762 252 153 9 34 264 55 303 1.63e-22 96.7
MS.gene027586.t1 AT5G64470 29.762 252 153 9 34 264 55 303 2.40e-22 96.3

Find 69 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCCTGGATCCAAGTATT+TGG 0.171032 5.1:+25687375 MS.gene027586:CDS
ATTATTGCAGTGGATCAAAA+TGG 0.241073 5.1:+25687311 MS.gene027586:intron
TACTTGAGATAGAACTTATC+TGG 0.342249 5.1:-25683096 None:intergenic
TTGCAGATGGGATTTCATTC+AGG 0.342474 5.1:+25686414 MS.gene027586:intron
AAAAGCATATTCCAAGTATC+AGG 0.342861 5.1:-25687574 None:intergenic
GATTTCTTAACAAGACTTAG+AGG 0.343900 5.1:+25685420 MS.gene027586:CDS
TTTCTCTACCTTTACCTTCC+CGG 0.347701 5.1:+25685557 MS.gene027586:CDS
TTTCCTGGATCCAAGTATTT+GGG 0.360598 5.1:+25687376 MS.gene027586:CDS
TGCAGCCATTGGTGTCTGCC+TGG 0.383241 5.1:+25687547 MS.gene027586:CDS
TGCCTGGTGTTCCTGATACT+TGG 0.385020 5.1:+25687563 MS.gene027586:CDS
CAAAAGCAATACACCAGATA+TGG 0.385433 5.1:-25682908 None:intergenic
ATATGCATCTACATCTTGTA+AGG 0.393113 5.1:+25687339 MS.gene027586:CDS
ATACCCAGCTGAGTTTGCTT+TGG 0.399109 5.1:+25687405 MS.gene027586:CDS
ACATATTGATATTTAACTCT+TGG 0.407854 5.1:+25686276 MS.gene027586:CDS
AAATACTTGGATCCAGGAAA+TGG 0.408011 5.1:-25687373 None:intergenic
GCTCACCCATCTGTATATAC+TGG 0.409600 5.1:+25687502 MS.gene027586:CDS
GGAAAGAGTCTCTTGTTTGT+TGG 0.412713 5.1:+25685441 MS.gene027586:CDS
TATGGTCAGGTGAGATTCCT+TGG 0.418149 5.1:-25686552 None:intergenic
TTCTCCAAAGCAAACTCAGC+TGG 0.437024 5.1:-25687409 None:intergenic
AGTTCTATCTCAAGTATAGA+TGG 0.438438 5.1:+25683103 MS.gene027586:CDS
ACTCCTTGAGTTTGAATCAA+TGG 0.440409 5.1:+25685466 MS.gene027586:CDS
CCAATGGCTGCAACTTTCCT+AGG 0.440858 5.1:+25683130 MS.gene027586:CDS
CCTAGGAAAGTTGCAGCCAT+TGG 0.443231 5.1:-25683130 None:intergenic
TTATTGCAGTGGATCAAAAT+GGG 0.446967 5.1:+25687312 MS.gene027586:intron
GTATGGACCTCCCAAATACT+TGG 0.450171 5.1:-25687386 None:intergenic
ATACAAAGACATGAATCGTT+TGG 0.452117 5.1:+25686450 MS.gene027586:CDS
ACATTGTAAGTGAACGTATT+GGG 0.456349 5.1:+25686201 MS.gene027586:CDS
CTAAAATTAGATTCAATTGA+AGG 0.459132 5.1:+25686230 MS.gene027586:CDS
ACACCAATGGCTGCAGTCAT+TGG 0.459885 5.1:-25687539 None:intergenic
TATTTAACTCTTGGCATTGG+TGG 0.469517 5.1:+25686285 MS.gene027586:CDS
CTAGGAAAGTTGCAGCCATT+GGG 0.477708 5.1:-25683129 None:intergenic
AAAGCAAACTCAGCTGGGTA+TGG 0.480269 5.1:-25687403 None:intergenic
TGATATTTAACTCTTGGCAT+TGG 0.480479 5.1:+25686282 MS.gene027586:CDS
TTACCACCTTATCAAAATTG+AGG 0.481938 5.1:+25687473 MS.gene027586:CDS
GACATTGTAAGTGAACGTAT+TGG 0.489693 5.1:+25686200 MS.gene027586:CDS
CATTCTGAGCTTTCATAAAG+AGG 0.492435 5.1:-25683036 None:intergenic
TGGCTTGTTTGTAGATTCAA+TGG 0.495819 5.1:+25685393 MS.gene027586:intron
CCTCCCAAATACTTGGATCC+AGG 0.505728 5.1:-25687379 None:intergenic
AAGAATGGAATTGCAAATAG+AGG 0.507153 5.1:+25682970 MS.gene027586:CDS
CAATTGAAGGAGGAAGACTA+TGG 0.509859 5.1:+25686243 MS.gene027586:CDS
AGATCTCACCGGGAAGGTAA+AGG 0.512881 5.1:-25685565 None:intergenic
AAAGGTAGAGAAACCTTTGT+TGG 0.517195 5.1:-25685547 None:intergenic
TTGGCATTGGTGGCTTCACA+CGG 0.534349 5.1:+25686295 MS.gene027586:CDS
GAGAATGGATTCAAGTGTGC+TGG 0.537595 5.1:+25683069 MS.gene027586:CDS
CCATGTCCAGTATATACAGA+TGG 0.552564 5.1:-25687508 None:intergenic
AAAACAAAGGTATTCTTCCA+AGG 0.564139 5.1:+25686535 MS.gene027586:CDS
TCTCCAAAGCAAACTCAGCT+GGG 0.571289 5.1:-25687408 None:intergenic
ATTCTGAGCTTTCATAAAGA+GGG 0.572418 5.1:-25683035 None:intergenic
ATACCAATGACTGCAGCCAT+TGG 0.577685 5.1:+25687536 MS.gene027586:CDS
AAGTATAGATGGCAGCCCAA+TGG 0.587504 5.1:+25683114 MS.gene027586:CDS
TATGCATCTACATCTTGTAA+GGG 0.588457 5.1:+25687340 MS.gene027586:CDS
TTGCCATTGATTCAAACTCA+AGG 0.588655 5.1:-25685469 None:intergenic
GAAGGAGGAAGACTATGGAA+AGG 0.590329 5.1:+25686248 MS.gene027586:CDS
AGATGGGATTTCATTCAGGA+AGG 0.593260 5.1:+25686418 MS.gene027586:intron
CCTGGATCCAAGTATTTGGG+AGG 0.603347 5.1:+25687379 MS.gene027586:CDS
TGGCATTGGTGGCTTCACAC+GGG 0.603454 5.1:+25686296 MS.gene027586:CDS
TACATCTCAGTCACCAACAA+AGG 0.605171 5.1:+25685534 MS.gene027586:CDS
CCATCTGTATATACTGGACA+TGG 0.606025 5.1:+25687508 MS.gene027586:CDS
CATGTCCAGTATATACAGAT+GGG 0.613179 5.1:-25687507 None:intergenic
GTAATATTGAGCAAATGCAC+AGG 0.615565 5.1:-25687454 None:intergenic
TTCCAAGTATCAGGAACACC+AGG 0.616906 5.1:-25687565 None:intergenic
GAACACCAGGCAGACACCAA+TGG 0.622052 5.1:-25687552 None:intergenic
ATGCATCTACATCTTGTAAG+GGG 0.627882 5.1:+25687341 MS.gene027586:CDS
ACTGAGATGTACTTGATATG+TGG 0.635687 5.1:-25685522 None:intergenic
TGTTGATCCTAGCAAAACAA+AGG 0.637400 5.1:+25686522 MS.gene027586:CDS
AAATTAGATTCAATTGAAGG+AGG 0.640627 5.1:+25686233 MS.gene027586:CDS
TGAAGAAGATCTCACCGGGA+AGG 0.646618 5.1:-25685571 None:intergenic
TATTGCAGTGGATCAAAATG+GGG 0.660632 5.1:+25687313 MS.gene027586:intron
TCTGTATATACTGGACATGG+TGG 0.667740 5.1:+25687511 MS.gene027586:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTAATTATTTTTTATA+GGG - chr5.1:25683662-25683681 None:intergenic 0.0%
!!! TATTATTAATTATTTTTTAT+AGG - chr5.1:25683663-25683682 None:intergenic 0.0%
!! TATATAACTCTATATAATTT+AGG - chr5.1:25687070-25687089 None:intergenic 10.0%
!! TGATACTAAAAAATAATTAA+AGG - chr5.1:25685175-25685194 None:intergenic 10.0%
!!! GATTAACATAATTAAATTTT+TGG - chr5.1:25685267-25685286 None:intergenic 10.0%
!!! GTATTTTTTTTTTTTTTTTG+AGG + chr5.1:25684270-25684289 MS.gene027586:intron 10.0%
!!! GTTCAATTAATTTTATAATT+TGG - chr5.1:25687241-25687260 None:intergenic 10.0%
!!! TAATCATTTATTTATCTTTT+TGG + chr5.1:25685734-25685753 MS.gene027586:intron 10.0%
!!! TATATTATGAATTTTTTAAG+TGG - chr5.1:25684039-25684058 None:intergenic 10.0%
!!! TTTAAAATATACTTATAAGA+TGG - chr5.1:25687163-25687182 None:intergenic 10.0%
!! AAAAAAAAAAAACGTTAGAA+AGG + chr5.1:25687129-25687148 MS.gene027586:intron 15.0%
!! AAAAAAAAAAACGTTAGAAA+GGG + chr5.1:25687130-25687149 MS.gene027586:intron 15.0%
!! AAAAATAATTAAAGGACAAA+AGG - chr5.1:25685167-25685186 None:intergenic 15.0%
!! AAAAATTGAAATATTGAACT+TGG + chr5.1:25685373-25685392 MS.gene027586:intron 15.0%
!! ACTATAGTACATTTAAATTA+TGG + chr5.1:25685854-25685873 MS.gene027586:intron 15.0%
!! CATTCAAAAAAAGTAAAAAA+AGG + chr5.1:25686002-25686021 MS.gene027586:intron 15.0%
!! TATATGCTAAATAATAGTAA+AGG - chr5.1:25685601-25685620 None:intergenic 15.0%
!! TATGGTATAATAATTTCAAA+TGG + chr5.1:25685337-25685356 MS.gene027586:intron 15.0%
!! TCTTAAAAAAATATATATCC+AGG - chr5.1:25686599-25686618 None:intergenic 15.0%
!!! AAAGAATTTTTTTATCACTA+TGG + chr5.1:25685319-25685338 MS.gene027586:intron 15.0%
!!! AAATATGTACTTACTTTTTA+TGG - chr5.1:25686573-25686592 None:intergenic 15.0%
!!! ATTATTTTTTAGTATCACTT+TGG + chr5.1:25685178-25685197 MS.gene027586:intron 15.0%
!! AAGGTTACATAAATAAGTAA+GGG - chr5.1:25684183-25684202 None:intergenic 20.0%
!! AATAAAGAGAAATCCAAATA+AGG - chr5.1:25684202-25684221 None:intergenic 20.0%
!! ACATATTGATATTTAACTCT+TGG + chr5.1:25686276-25686295 MS.gene027586:CDS 20.0%
!! ACTTCGTTACATATAAAAAA+GGG + chr5.1:25683511-25683530 MS.gene027586:intron 20.0%
!! AGTATTTATCTTATTGTTCA+AGG + chr5.1:25686999-25687018 MS.gene027586:intron 20.0%
!! ATAAAACTTACCAAGTATAT+TGG + chr5.1:25687038-25687057 MS.gene027586:intron 20.0%
!! ATATAGAGTTATATACAACA+GGG + chr5.1:25687075-25687094 MS.gene027586:intron 20.0%
!! ATATGACATGACAAATTATA+TGG - chr5.1:25685104-25685123 None:intergenic 20.0%
!! CAAATTATAATAGGAATAAG+TGG - chr5.1:25686132-25686151 None:intergenic 20.0%
!! CTAAAATTAGATTCAATTGA+AGG + chr5.1:25686230-25686249 MS.gene027586:CDS 20.0%
!! CTTATTTATGTAACCTTATT+TGG + chr5.1:25684186-25684205 MS.gene027586:intron 20.0%
!! TAAATAGTAGCAAAGAAAAA+AGG - chr5.1:25683252-25683271 None:intergenic 20.0%
!! TAAGGTTACATAAATAAGTA+AGG - chr5.1:25684184-25684203 None:intergenic 20.0%
!! TAGTTCTTGAAAAAATTGAA+TGG - chr5.1:25685838-25685857 None:intergenic 20.0%
!! TATATAGAGTTATATACAAC+AGG + chr5.1:25687074-25687093 MS.gene027586:intron 20.0%
!! TCACAACAAAATAGTATATT+AGG + chr5.1:25685131-25685150 MS.gene027586:intron 20.0%
!! TCTTTAAATAATTATTGCAG+TGG + chr5.1:25687301-25687320 MS.gene027586:intron 20.0%
!! TGAACAATAAGATAAATACT+AGG - chr5.1:25686999-25687018 None:intergenic 20.0%
!! TGAAGGAAAAAAAAAATACT+TGG - chr5.1:25686106-25686125 None:intergenic 20.0%
!! TTGTTATGTTGAACATATTT+AGG + chr5.1:25683429-25683448 MS.gene027586:intron 20.0%
!!! AAATTCTTTTTTTTTGCACA+AGG - chr5.1:25685308-25685327 None:intergenic 20.0%
!!! AACATAATGTCTTCAAATTT+TGG + chr5.1:25684664-25684683 MS.gene027586:intron 20.0%
!!! AACATTGACTTAAAAATTTG+AGG - chr5.1:25683279-25683298 None:intergenic 20.0%
!!! ACATAATGTCTTCAAATTTT+GGG + chr5.1:25684665-25684684 MS.gene027586:intron 20.0%
!!! ACATTGACTTAAAAATTTGA+GGG - chr5.1:25683278-25683297 None:intergenic 20.0%
!!! CGTTTTAACAACAAATAAAT+AGG + chr5.1:25684059-25684078 MS.gene027586:intron 20.0%
!!! CTAATTAAGCACTTTTTTTA+AGG - chr5.1:25685031-25685050 None:intergenic 20.0%
!!! CTTCAATTGAATCTAATTTT+AGG - chr5.1:25686232-25686251 None:intergenic 20.0%
!!! TAATTTATAATAAAGTGCTC+CGG - chr5.1:25683554-25683573 None:intergenic 20.0%
!!! TACTGTTGATTAAAATTTGA+TGG - chr5.1:25683339-25683358 None:intergenic 20.0%
!!! TGATTTATTTTATGGTAAGT+GGG + chr5.1:25682996-25683015 MS.gene027586:CDS 20.0%
!!! TTAGAATAAGTTTAAAATCC+AGG - chr5.1:25684932-25684951 None:intergenic 20.0%
!!! TTTATGAAGAATTAAGTTGT+TGG - chr5.1:25687206-25687225 None:intergenic 20.0%
!!! TTTTTAAGTCAATGTTATTG+TGG + chr5.1:25683283-25683302 MS.gene027586:intron 20.0%
!!! TTTTTTTTGAGGAAAAAATG+AGG + chr5.1:25684281-25684300 MS.gene027586:intron 20.0%
! AAACAAGAACACTATTAGAT+TGG + chr5.1:25683805-25683824 MS.gene027586:intron 25.0%
! AAATTAGATTCAATTGAAGG+AGG + chr5.1:25686233-25686252 MS.gene027586:CDS 25.0%
! AATAGTTGTATCAAAGTCAA+TGG + chr5.1:25684435-25684454 MS.gene027586:intron 25.0%
! AATGAATTAGTGAGCATTTA+TGG + chr5.1:25683708-25683727 MS.gene027586:intron 25.0%
! AATGACATGTCTAATTTAGA+AGG - chr5.1:25683164-25683183 None:intergenic 25.0%
! AATTACATGATTCTATAACC+TGG + chr5.1:25684911-25684930 MS.gene027586:intron 25.0%
! ACATAAATAAGTAAGGGAAA+TGG - chr5.1:25684177-25684196 None:intergenic 25.0%
! ATAAATGCTCACTAATTCAT+TGG - chr5.1:25683709-25683728 None:intergenic 25.0%
! ATAGATAGACTTATACTTGA+AGG + chr5.1:25684552-25684571 MS.gene027586:intron 25.0%
! ATGAATTAGTGAGCATTTAT+GGG + chr5.1:25683709-25683728 MS.gene027586:intron 25.0%
! ATGAATTTAAAGGACTCAAA+CGG - chr5.1:25685224-25685243 None:intergenic 25.0%
! ATGACATGTCTAATTTAGAA+GGG - chr5.1:25683163-25683182 None:intergenic 25.0%
! ATGAGGTAGTATTATACATA+TGG + chr5.1:25684298-25684317 MS.gene027586:intron 25.0%
! ATGATGTGTAATTCTATTTG+AGG + chr5.1:25686070-25686089 MS.gene027586:intron 25.0%
! ATTAAACATATGTACACACT+AGG - chr5.1:25685072-25685091 None:intergenic 25.0%
! CAACTCTTCTTCATAATTAA+AGG - chr5.1:25684771-25684790 None:intergenic 25.0%
! CACTTCGTTACATATAAAAA+AGG + chr5.1:25683510-25683529 MS.gene027586:intron 25.0%
! CTAGTAACTAATAAACACTA+AGG + chr5.1:25683835-25683854 MS.gene027586:intron 25.0%
! GATAGAAAAATGAATGTTCA+AGG + chr5.1:25684618-25684637 MS.gene027586:intron 25.0%
! GGAAAAAAAAAATACTTGGT+TGG - chr5.1:25686102-25686121 None:intergenic 25.0%
! TAAAAAAGAGACAACAGTAA+GGG + chr5.1:25686926-25686945 MS.gene027586:intron 25.0%
! TAGGTAAATTCCAATATACT+TGG - chr5.1:25687051-25687070 None:intergenic 25.0%
! TGCTAAATAATAGTAAAGGT+AGG - chr5.1:25685597-25685616 None:intergenic 25.0%
! TGGAATTATACTGTTGAAAT+TGG - chr5.1:25687186-25687205 None:intergenic 25.0%
! TTCTTCATAATTAAAGGTAG+AGG - chr5.1:25684765-25684784 None:intergenic 25.0%
!! AATTTATAATAAAGTGCTCC+GGG - chr5.1:25683553-25683572 None:intergenic 25.0%
!! AGATGTAATTTTCTATTGAG+AGG - chr5.1:25683993-25684012 None:intergenic 25.0%
!! ATGTATTTTTCTCATCCAAT+TGG + chr5.1:25683188-25683207 MS.gene027586:intron 25.0%
!! CTCTTTTCATTCAAATACAT+GGG + chr5.1:25682932-25682951 MS.gene027586:CDS 25.0%
!! TATAACGGAGATGAATTTAA+AGG - chr5.1:25685234-25685253 None:intergenic 25.0%
!! TCTCTTTTCATTCAAATACA+TGG + chr5.1:25682931-25682950 MS.gene027586:CDS 25.0%
!! TGTATTCTATGTTTTCTAAG+AGG + chr5.1:25683895-25683914 MS.gene027586:intron 25.0%
!! TTTTCTTATACTTTGCAGAT+GGG + chr5.1:25686402-25686421 MS.gene027586:intron 25.0%
!!! ACAAGAAAGCTTTAAAAACA+TGG + chr5.1:25686479-25686498 MS.gene027586:CDS 25.0%
!!! AGAGGTTGTGATTTATTTTA+TGG + chr5.1:25682988-25683007 MS.gene027586:CDS 25.0%
!!! ATCTTTCCTCAATTTTGATA+AGG - chr5.1:25687482-25687501 None:intergenic 25.0%
!!! ATTCATTTTTCTATCTAACG+TGG - chr5.1:25684613-25684632 None:intergenic 25.0%
!!! CAAGAAAGCTTTAAAAACAT+GGG + chr5.1:25686480-25686499 MS.gene027586:CDS 25.0%
!!! GTATTCTATGTTTTCTAAGA+GGG + chr5.1:25683896-25683915 MS.gene027586:intron 25.0%
!!! GTGATTTATTTTATGGTAAG+TGG + chr5.1:25682995-25683014 MS.gene027586:CDS 25.0%
!!! TCAAATTTTGGGTTAAAATG+TGG + chr5.1:25684676-25684695 MS.gene027586:intron 25.0%
!!! TCTCTTTTTTACAATTGAGA+TGG - chr5.1:25686917-25686936 None:intergenic 25.0%
!!! TCTTTTGAAAAAATCGTGTA+TGG + chr5.1:25685948-25685967 MS.gene027586:intron 25.0%
!!! TTCATTTTTCTATCTAACGT+GGG - chr5.1:25684612-25684631 None:intergenic 25.0%
!!! TTTTGGTTTCAAGAGTTTAA+CGG + chr5.1:25685751-25685770 MS.gene027586:intron 25.0%
AAGAATGGAATTGCAAATAG+AGG + chr5.1:25682970-25682989 MS.gene027586:CDS 30.0%
ACATTGTAAGTGAACGTATT+GGG + chr5.1:25686201-25686220 MS.gene027586:CDS 30.0%
ACTGGTTAAGGATATGAAAA+AGG - chr5.1:25683614-25683633 None:intergenic 30.0%
AGAGATTGTTGTGTATCATA+TGG + chr5.1:25684575-25684594 MS.gene027586:intron 30.0%
AGTTCTATCTCAAGTATAGA+TGG + chr5.1:25683103-25683122 MS.gene027586:CDS 30.0%
ATACAAAGACATGAATCGTT+TGG + chr5.1:25686450-25686469 MS.gene027586:CDS 30.0%
ATATGCATCTACATCTTGTA+AGG + chr5.1:25687339-25687358 MS.gene027586:CDS 30.0%
ATTATTGCAGTGGATCAAAA+TGG + chr5.1:25687311-25687330 MS.gene027586:intron 30.0%
ATTCTGAGCTTTCATAAAGA+GGG - chr5.1:25683038-25683057 None:intergenic 30.0%
CTCTTTCTTTCCCATATAAA+AGG - chr5.1:25683748-25683767 None:intergenic 30.0%
GATACAACTATTGAATCATG+AGG - chr5.1:25684427-25684446 None:intergenic 30.0%
GATTTCTTAACAAGACTTAG+AGG + chr5.1:25685420-25685439 MS.gene027586:CDS 30.0%
GTAAAAAAGAGACAACAGTA+AGG + chr5.1:25686925-25686944 MS.gene027586:intron 30.0%
GTACTTTCTTGATCTATTCT+TGG - chr5.1:25686659-25686678 None:intergenic 30.0%
TACTTGAGATAGAACTTATC+TGG - chr5.1:25683099-25683118 None:intergenic 30.0%
TATGCATCTACATCTTGTAA+GGG + chr5.1:25687340-25687359 MS.gene027586:CDS 30.0%
TCTGCAAAGTATAAGAAAAG+AGG - chr5.1:25686401-25686420 None:intergenic 30.0%
TGAATTAGTGAGCATTTATG+GGG + chr5.1:25683710-25683729 MS.gene027586:intron 30.0%
TGATATCCAACTTACTTGTA+GGG + chr5.1:25684364-25684383 MS.gene027586:intron 30.0%
TGATATTTAACTCTTGGCAT+TGG + chr5.1:25686282-25686301 MS.gene027586:CDS 30.0%
TGTCGATCTTTCACTATATT+TGG - chr5.1:25686804-25686823 None:intergenic 30.0%
TTACCACCTTATCAAAATTG+AGG + chr5.1:25687473-25687492 MS.gene027586:CDS 30.0%
TTATTGCAGTGGATCAAAAT+GGG + chr5.1:25687312-25687331 MS.gene027586:intron 30.0%
TTCAAGGCAATACAAAACAA+AGG + chr5.1:25687015-25687034 MS.gene027586:intron 30.0%
TTCATCTCCGTTATATAAGT+TGG + chr5.1:25685239-25685258 MS.gene027586:intron 30.0%
TTGAATCCATTCTCCAAAAA+TGG - chr5.1:25683063-25683082 None:intergenic 30.0%
TTTACAATTGAGATGGTTCA+TGG - chr5.1:25686910-25686929 None:intergenic 30.0%
! AAAAGCATATTCCAAGTATC+AGG - chr5.1:25687577-25687596 None:intergenic 30.0%
! ACTTTGATCTCGAACTTTTA+TGG - chr5.1:25686695-25686714 None:intergenic 30.0%
! AGTAAGTACATATTTTCTCC+TGG + chr5.1:25686578-25686597 MS.gene027586:intron 30.0%
! AGTGATAGTTTTCACAAGAA+TGG + chr5.1:25682955-25682974 MS.gene027586:CDS 30.0%
! ATTTATAATAAAGTGCTCCG+GGG - chr5.1:25683552-25683571 None:intergenic 30.0%
! CAAAACTGTTGTTGATCTTT+TGG - chr5.1:25683873-25683892 None:intergenic 30.0%
! CAAAAGATCAACAACAGTTT+TGG + chr5.1:25683871-25683890 MS.gene027586:intron 30.0%
! CTTTTCTTATACTTTGCAGA+TGG + chr5.1:25686401-25686420 MS.gene027586:intron 30.0%
! GTAGCTCTGCAAATTATAAT+AGG - chr5.1:25686141-25686160 None:intergenic 30.0%
! GTTGAAATAAGCTTTGTGAA+TGG - chr5.1:25683395-25683414 None:intergenic 30.0%
! TTTGGGACCAACTTATATAA+CGG - chr5.1:25685249-25685268 None:intergenic 30.0%
! TTTTATAGGGAAATGCTAAC+CGG - chr5.1:25683649-25683668 None:intergenic 30.0%
!! AAAACAAAGGTATTCTTCCA+AGG + chr5.1:25686535-25686554 MS.gene027586:CDS 30.0%
!! TTTCCTCAATTTTGATAAGG+TGG - chr5.1:25687479-25687498 None:intergenic 30.0%
!!! AAGAATACCTTTGTTTTGCT+AGG - chr5.1:25686532-25686551 None:intergenic 30.0%
!!! ATGAACTAGATTTTAACTCG+AGG - chr5.1:25686778-25686797 None:intergenic 30.0%
!!! TGAACTAGATTTTAACTCGA+GGG - chr5.1:25686777-25686796 None:intergenic 30.0%
!!! TGTACTTACTTTTTATGGTC+AGG - chr5.1:25686568-25686587 None:intergenic 30.0%
AAAGGTAGAGAAACCTTTGT+TGG - chr5.1:25685550-25685569 None:intergenic 35.0%
AAATACTTGGATCCAGGAAA+TGG - chr5.1:25687376-25687395 None:intergenic 35.0%
AAGCTCATAAATGTAGCGTT+AGG + chr5.1:25684095-25684114 MS.gene027586:intron 35.0%
ACAACTATTGAATCATGAGG+AGG - chr5.1:25684424-25684443 None:intergenic 35.0%
ACACACTTGTATCTCAACAT+AGG - chr5.1:25684144-25684163 None:intergenic 35.0%
ACTCCTTGAGTTTGAATCAA+TGG + chr5.1:25685466-25685485 MS.gene027586:CDS 35.0%
ACTGAGATGTACTTGATATG+TGG - chr5.1:25685525-25685544 None:intergenic 35.0%
AGTAGCAAAGAAAAAAGGCA+CGG - chr5.1:25683247-25683266 None:intergenic 35.0%
ATAGGAATAAGTGGTCATGA+AGG - chr5.1:25686123-25686142 None:intergenic 35.0%
ATATCCAACTTACTTGTAGG+GGG + chr5.1:25684366-25684385 MS.gene027586:intron 35.0%
ATGCATCTACATCTTGTAAG+GGG + chr5.1:25687341-25687360 MS.gene027586:CDS 35.0%
ATTTCCTGGATCCAAGTATT+TGG + chr5.1:25687375-25687394 MS.gene027586:CDS 35.0%
CAAAAGCAATACACCAGATA+TGG - chr5.1:25682911-25682930 None:intergenic 35.0%
CAACTATTGAATCATGAGGA+GGG - chr5.1:25684423-25684442 None:intergenic 35.0%
CATGTCCAGTATATACAGAT+GGG - chr5.1:25687510-25687529 None:intergenic 35.0%
CATGTGAGGAGTGAAAAATT+AGG - chr5.1:25686337-25686356 None:intergenic 35.0%
CATTCTGAGCTTTCATAAAG+AGG - chr5.1:25683039-25683058 None:intergenic 35.0%
GACATTGTAAGTGAACGTAT+TGG + chr5.1:25686200-25686219 MS.gene027586:CDS 35.0%
GATATCCAACTTACTTGTAG+GGG + chr5.1:25684365-25684384 MS.gene027586:intron 35.0%
GTAATATTGAGCAAATGCAC+AGG - chr5.1:25687457-25687476 None:intergenic 35.0%
GTGATATCCAACTTACTTGT+AGG + chr5.1:25684363-25684382 MS.gene027586:intron 35.0%
TAAAAAAGGGTCCTCTTAAC+CGG + chr5.1:25683524-25683543 MS.gene027586:intron 35.0%
TATTGCAGTGGATCAAAATG+GGG + chr5.1:25687313-25687332 MS.gene027586:intron 35.0%
TGTCCACCAAAAATGCATTA+CGG - chr5.1:25686184-25686203 None:intergenic 35.0%
TGTTGATCCTAGCAAAACAA+AGG + chr5.1:25686522-25686541 MS.gene027586:CDS 35.0%
TTTAAAAACATGGGCGAAGT+GGG + chr5.1:25686489-25686508 MS.gene027586:CDS 35.0%
TTTCCTGGATCCAAGTATTT+GGG + chr5.1:25687376-25687395 MS.gene027586:CDS 35.0%
! AAGCTCAGAATGTCCATTTT+TGG + chr5.1:25683047-25683066 MS.gene027586:CDS 35.0%
! GTTTTCCCGTAATGCATTTT+TGG + chr5.1:25686175-25686194 MS.gene027586:CDS 35.0%
! GTTTTGTGCGCCTTTTATAT+GGG + chr5.1:25683735-25683754 MS.gene027586:intron 35.0%
! TATTTAACTCTTGGCATTGG+TGG + chr5.1:25686285-25686304 MS.gene027586:CDS 35.0%
!! AGTTTTGTGCGCCTTTTATA+TGG + chr5.1:25683734-25683753 MS.gene027586:intron 35.0%
!! ATGGGGTTTTATACCATATC+TGG + chr5.1:25682895-25682914 MS.gene027586:CDS 35.0%
!! GAATGTCCATTTTTGGAGAA+TGG + chr5.1:25683054-25683073 MS.gene027586:CDS 35.0%
!! GGATAGACGTTTCTAAAATG+TGG + chr5.1:25684245-25684264 MS.gene027586:intron 35.0%
!! TGGCTTGTTTGTAGATTCAA+TGG + chr5.1:25685393-25685412 MS.gene027586:intron 35.0%
!! TTGCCATTGATTCAAACTCA+AGG - chr5.1:25685472-25685491 None:intergenic 35.0%
!!! AGTTTTGTTGTGCTCATCTT+GGG + chr5.1:25683766-25683785 MS.gene027586:intron 35.0%
AACCCATAAATTCAACCGCA+AGG - chr5.1:25683475-25683494 None:intergenic 40.0%
ACTTGTATCTCAACATAGGC+AGG - chr5.1:25684140-25684159 None:intergenic 40.0%
AGACCTTGCGGTTGAATTTA+TGG + chr5.1:25683469-25683488 MS.gene027586:intron 40.0%
AGATGGGATTTCATTCAGGA+AGG + chr5.1:25686418-25686437 MS.gene027586:intron 40.0%
AGCATGCATCACTATCCAAT+TGG - chr5.1:25683206-25683225 None:intergenic 40.0%
AGGGTCATCAACGTTAGAAA+GGG + chr5.1:25687094-25687113 MS.gene027586:intron 40.0%
ATGAAAAAGCCTCCACTTGA+GGG + chr5.1:25684509-25684528 MS.gene027586:intron 40.0%
ATTGAGAGGAGTAGTAAACG+TGG - chr5.1:25683979-25683998 None:intergenic 40.0%
CAATTGAAGGAGGAAGACTA+TGG + chr5.1:25686243-25686262 MS.gene027586:CDS 40.0%
CCATCTGTATATACTGGACA+TGG + chr5.1:25687508-25687527 MS.gene027586:CDS 40.0%
CCATGTCCAGTATATACAGA+TGG - chr5.1:25687511-25687530 None:intergenic 40.0%
CGGTTGAATTTATGGGTTTC+AGG + chr5.1:25683477-25683496 MS.gene027586:intron 40.0%
GACCTTGCGGTTGAATTTAT+GGG + chr5.1:25683470-25683489 MS.gene027586:intron 40.0%
GCGACAACAATAAGTGTATG+TGG + chr5.1:25684486-25684505 MS.gene027586:intron 40.0%
GTCCACCAAAAATGCATTAC+GGG - chr5.1:25686183-25686202 None:intergenic 40.0%
GTGAAAGATCGACAACATTG+AGG + chr5.1:25686810-25686829 MS.gene027586:intron 40.0%
TAAATTCAACCGCAAGGTCT+CGG - chr5.1:25683469-25683488 None:intergenic 40.0%
TACATCTCAGTCACCAACAA+AGG + chr5.1:25685534-25685553 MS.gene027586:CDS 40.0%
TCGAGATCAAAGTAACGACA+AGG + chr5.1:25686702-25686721 MS.gene027586:intron 40.0%
TCTGTATATACTGGACATGG+TGG + chr5.1:25687511-25687530 MS.gene027586:CDS 40.0%
TGTTAAGAGTAGTGCATGTG+AGG - chr5.1:25686351-25686370 None:intergenic 40.0%
TTGCAGATGGGATTTCATTC+AGG + chr5.1:25686414-25686433 MS.gene027586:intron 40.0%
TTTAGAAGGGATGTGTACCT+AGG - chr5.1:25683150-25683169 None:intergenic 40.0%
TTTCTCTACCTTTACCTTCC+CGG + chr5.1:25685557-25685576 MS.gene027586:CDS 40.0%
! AAGGTAGAGGTACACGTTAT+TGG - chr5.1:25684752-25684771 None:intergenic 40.0%
! CTTTAAAAACATGGGCGAAG+TGG + chr5.1:25686488-25686507 MS.gene027586:CDS 40.0%
! GGAAAGAGTCTCTTGTTTGT+TGG + chr5.1:25685441-25685460 MS.gene027586:CDS 40.0%
! GTAGCAAAGAAAAAAGGCAC+GGG - chr5.1:25683246-25683265 None:intergenic 40.0%
! TAGCAAAGAAAAAAGGCACG+GGG - chr5.1:25683245-25683264 None:intergenic 40.0%
!! TTCCCGTAATGCATTTTTGG+TGG + chr5.1:25686178-25686197 MS.gene027586:CDS 40.0%
!!! GAGTTTTGTTGTGCTCATCT+TGG + chr5.1:25683765-25683784 MS.gene027586:intron 40.0%
AAAGCAAACTCAGCTGGGTA+TGG - chr5.1:25687406-25687425 None:intergenic 45.0%
AAGTATAGATGGCAGCCCAA+TGG + chr5.1:25683114-25683133 MS.gene027586:CDS 45.0%
ACACGTTATTGGATCTTGCG+AGG - chr5.1:25684741-25684760 None:intergenic 45.0%
AGATGGTTCATGGTTCCACA+AGG - chr5.1:25686900-25686919 None:intergenic 45.0%
AGGTCTCGGATTCAAACTCA+AGG - chr5.1:25683455-25683474 None:intergenic 45.0%
AGTTTGAATCCGAGACCTTG+CGG + chr5.1:25683457-25683476 MS.gene027586:intron 45.0%
ATACCAATGACTGCAGCCAT+TGG + chr5.1:25687536-25687555 MS.gene027586:CDS 45.0%
CAGGGTCATCAACGTTAGAA+AGG + chr5.1:25687093-25687112 MS.gene027586:intron 45.0%
CTAGGAAAGTTGCAGCCATT+GGG - chr5.1:25683132-25683151 None:intergenic 45.0%
GAAGGAGGAAGACTATGGAA+AGG + chr5.1:25686248-25686267 MS.gene027586:CDS 45.0%
GATCGACAACATTGAGGAGA+AGG + chr5.1:25686816-25686835 MS.gene027586:intron 45.0%
GATGAAAAAGCCTCCACTTG+AGG + chr5.1:25684508-25684527 MS.gene027586:intron 45.0%
GCTCACCCATCTGTATATAC+TGG + chr5.1:25687502-25687521 MS.gene027586:CDS 45.0%
GGGCAAAAACTTCCATTTCC+TGG + chr5.1:25687361-25687380 MS.gene027586:CDS 45.0%
GGGCTAGAGAATCATCTCAT+TGG - chr5.1:25684403-25684422 None:intergenic 45.0%
GTAGGTGAAGAAGATCTCAC+CGG - chr5.1:25685579-25685598 None:intergenic 45.0%
GTATGGACCTCCCAAATACT+TGG - chr5.1:25687389-25687408 None:intergenic 45.0%
TAGGTGAAGAAGATCTCACC+GGG - chr5.1:25685578-25685597 None:intergenic 45.0%
TATGGTCAGGTGAGATTCCT+TGG - chr5.1:25686555-25686574 None:intergenic 45.0%
TCTCCAAAGCAAACTCAGCT+GGG - chr5.1:25687411-25687430 None:intergenic 45.0%
TGAGTATACTCTCCCTCAAG+TGG - chr5.1:25684524-25684543 None:intergenic 45.0%
TTCCAAGTATCAGGAACACC+AGG - chr5.1:25687568-25687587 None:intergenic 45.0%
TTCTCCAAAGCAAACTCAGC+TGG - chr5.1:25687412-25687431 None:intergenic 45.0%
! ATACCCAGCTGAGTTTGCTT+TGG + chr5.1:25687405-25687424 MS.gene027586:CDS 45.0%
! GAGAATGGATTCAAGTGTGC+TGG + chr5.1:25683069-25683088 MS.gene027586:CDS 45.0%
!! TACTTGTAGGGGGTTGCTTT+TGG + chr5.1:25684376-25684395 MS.gene027586:intron 45.0%
!! TTAATAAAAATGAAAAATAA+TGG + chr5.1:25684224-25684243 MS.gene027586:intron 5.0%
!!! ATTAACATAATTAAATTTTT+GGG - chr5.1:25685266-25685285 None:intergenic 5.0%
!!! TTTTTATTTTTTATTTATCT+TGG - chr5.1:25685909-25685928 None:intergenic 5.0%
ACACCAATGGCTGCAGTCAT+TGG - chr5.1:25687542-25687561 None:intergenic 50.0%
AGATCTCACCGGGAAGGTAA+AGG - chr5.1:25685568-25685587 None:intergenic 50.0%
ATATCCTTAACCAGTGCCCC+GGG + chr5.1:25683619-25683638 MS.gene027586:intron 50.0%
CATATCCTTAACCAGTGCCC+CGG + chr5.1:25683618-25683637 MS.gene027586:intron 50.0%
CCAATGGCTGCAACTTTCCT+AGG + chr5.1:25683130-25683149 MS.gene027586:CDS 50.0%
CCTAGGAAAGTTGCAGCCAT+TGG - chr5.1:25683133-25683152 None:intergenic 50.0%
CCTCCCAAATACTTGGATCC+AGG - chr5.1:25687382-25687401 None:intergenic 50.0%
CCTGGATCCAAGTATTTGGG+AGG + chr5.1:25687379-25687398 MS.gene027586:CDS 50.0%
CGACAACATTGAGGAGAAGG+AGG + chr5.1:25686819-25686838 MS.gene027586:intron 50.0%
GCAACCCCCTACAAGTAAGT+TGG - chr5.1:25684373-25684392 None:intergenic 50.0%
GTATACTCTCCCTCAAGTGG+AGG - chr5.1:25684521-25684540 None:intergenic 50.0%
TGAAGAAGATCTCACCGGGA+AGG - chr5.1:25685574-25685593 None:intergenic 50.0%
TGCCTGGTGTTCCTGATACT+TGG + chr5.1:25687563-25687582 MS.gene027586:CDS 50.0%
TGGATCTTGCGAGGAGTTCA+TGG - chr5.1:25684732-25684751 None:intergenic 50.0%
!! TGTAGCGTTAGGCGTCGAAT+CGG + chr5.1:25684106-25684125 MS.gene027586:intron 50.0%
!! TTGGCATTGGTGGCTTCACA+CGG + chr5.1:25686295-25686314 MS.gene027586:CDS 50.0%
AAATGCTAACCGGTGCTCCC+GGG - chr5.1:25683639-25683658 None:intergenic 55.0%
GAAATGCTAACCGGTGCTCC+CGG - chr5.1:25683640-25683659 None:intergenic 55.0%
GAACACCAGGCAGACACCAA+TGG - chr5.1:25687555-25687574 None:intergenic 55.0%
! AATAAAGTGCTCCGGGGCAC+CGG - chr5.1:25683546-25683565 None:intergenic 55.0%
!! GTAGCGTTAGGCGTCGAATC+GGG + chr5.1:25684107-25684126 MS.gene027586:intron 55.0%
!! TGGCATTGGTGGCTTCACAC+GGG + chr5.1:25686296-25686315 MS.gene027586:CDS 55.0%
AATGCTAACCGGTGCTCCCG+GGG - chr5.1:25683638-25683657 None:intergenic 60.0%
! TGCAGCCATTGGTGTCTGCC+TGG + chr5.1:25687547-25687566 MS.gene027586:CDS 60.0%
GGTCCTCTTAACCGGTGCCC+CGG + chr5.1:25683532-25683551 MS.gene027586:intron 65.0%
!! CGTTAGGCGTCGAATCGGGC+GGG + chr5.1:25684111-25684130 MS.gene027586:intron 65.0%
!! GCGTTAGGCGTCGAATCGGG+CGG + chr5.1:25684110-25684129 MS.gene027586:intron 65.0%
!! TGCTCCCGGGGCACTGGTTA+AGG - chr5.1:25683626-25683645 None:intergenic 65.0%
! AACCAGTGCCCCGGGAGCAC+CGG + chr5.1:25683627-25683646 MS.gene027586:intron 70.0%
!! GCTCCGGGGCACCGGTTAAG+AGG - chr5.1:25683538-25683557 None:intergenic 70.0%
AACCGGTGCTCCCGGGGCAC+TGG - chr5.1:25683632-25683651 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 25682895 25687621 25682895 ID=MS.gene027586
chr5.1 mRNA 25682895 25687621 25682895 ID=MS.gene027586.t1;Parent=MS.gene027586
chr5.1 exon 25682895 25683151 25682895 ID=MS.gene027586.t1.exon1;Parent=MS.gene027586.t1
chr5.1 CDS 25682895 25683151 25682895 ID=cds.MS.gene027586.t1;Parent=MS.gene027586.t1
chr5.1 exon 25685407 25685578 25685407 ID=MS.gene027586.t1.exon2;Parent=MS.gene027586.t1
chr5.1 CDS 25685407 25685578 25685407 ID=cds.MS.gene027586.t1;Parent=MS.gene027586.t1
chr5.1 exon 25686155 25686330 25686155 ID=MS.gene027586.t1.exon3;Parent=MS.gene027586.t1
chr5.1 CDS 25686155 25686330 25686155 ID=cds.MS.gene027586.t1;Parent=MS.gene027586.t1
chr5.1 exon 25686420 25686578 25686420 ID=MS.gene027586.t1.exon4;Parent=MS.gene027586.t1
chr5.1 CDS 25686420 25686578 25686420 ID=cds.MS.gene027586.t1;Parent=MS.gene027586.t1
chr5.1 exon 25687321 25687621 25687321 ID=MS.gene027586.t1.exon5;Parent=MS.gene027586.t1
chr5.1 CDS 25687321 25687621 25687321 ID=cds.MS.gene027586.t1;Parent=MS.gene027586.t1
Gene Sequence

>MS.gene027586

ATGGGGTTTTATACCATATCTGGTGTATTGCTTTTGTCTCTTTTCATTCAAATACATGGGAGTGATAGTTTTCACAAGAATGGAATTGCAAATAGAGGTTGTGATTTATTTTATGGTAAGTGGGTGTTTGATGATTCATACCCTCTTTATGAAAGCTCAGAATGTCCATTTTTGGAGAATGGATTCAAGTGTGCTGGTCGTCCAGATAAGTTCTATCTCAAGTATAGATGGCAGCCCAATGGCTGCAACTTTCCTAGGTACACATCCCTTCTAAATTAGACATGTCATTTACTATGTATTTTTCTCATCCAATTGGATAGTGATGCATGCTTAAATTTTCTTGTGATCCCCGTGCCTTTTTTCTTTGCTACTATTTATTACCCTCAAATTTTTAAGTCAATGTTATTGTGGCATCATTCTTTTTGATATTATGAACAATTGCCATCAAATTTTAATCAACAGTAGTTTTTATTTTTTATTTTTTATATAATTAACAACCATTCACAAAGCTTATTTCAACTGACAATGTTAATATTGTTATGTTGAACATATTTAGGCCTTGAGTTTGAATCCGAGACCTTGCGGTTGAATTTATGGGTTTCAGGTGATATTTGTCACTTCGTTACATATAAAAAAGGGTCCTCTTAACCGGTGCCCCGGAGCACTTTATTATAAATTAATTACACAATTTCAATGCATTAACTACTATATAATTTCCTTTTTCATATCCTTAACCAGTGCCCCGGGAGCACCGGTTAGCATTTCCCTATAAAAAATAATTAATAATATAATAATAATTAATAATGAACAACCAATGAATTAGTGAGCATTTATGGGGAAGTTTTGTGCGCCTTTTATATGGGAAAGAAAGAGTTTTGTTGTGCTCATCTTGGGCAGAATTAAAGCAGAAAAACAAGAACACTATTAGATTGGATCTCTACTAGTAACTAATAAACACTAAGGTTGATTAACGAACCAAAAGATCAACAACAGTTTTGGATGTATTCTATGTTTTCTAAGAGGGATGTATATACTATGATTATCATACTAGAAGAATTTACGCGTGACAATTAATTAGATTCCACGTTTACTACTCCTCTCAATAGAAAATTACATCTTTGAATTGTACTTGTTTTTTAAACCACTTAAAAAATTCATAATATACGTTTTAACAACAAATAAATAGGAATAAAATGAGACAAGCTCATAAATGTAGCGTTAGGCGTCGAATCGGGCGGGAATCCTGCCTATGTTGAGATACAAGTGTGTCATGTGTGAGCCATTTCCCTTACTTATTTATGTAACCTTATTTGGATTTCTCTTTATTAGTTAATAAAAATGAAAAATAATGGATAGACGTTTCTAAAATGTGGTAGTATTTTTTTTTTTTTTTTGAGGAAAAAATGAGGTAGTATTATACATATGGAGAGACACACAAACTTAATGTCTTAAGATTTTAGTTTAAAATGTGATATCCAACTTACTTGTAGGGGGTTGCTTTTGGTCCAATGAGATGATTCTCTAGCCCTCCTCATGATTCAATAGTTGTATCAAAGTCAATGGTGATCAAACGACGTGTTTAATTGATGCAGCGACAACAATAAGTGTATGTGGATGAAAAAGCCTCCACTTGAGGGAGAGTATACTCATATGTGAAATAGATAGACTTATACTTGAAGGAGAGATTGTTGTGTATCATATGGTAGTTAGAAGACCCACGTTAGATAGAAAAATGAATGTTCAAGGACATGTAAGTAAATAGACTAATAAACATAATGTCTTCAAATTTTGGGTTAAAATGTGGTGTCAAATTCGCTTGTGTGATTGCTATTGACCATGAACTCCTCGCAAGATCCAATAACGTGTACCTCTACCTTTAATTATGAAGAAGAGTTGAATATTTGAACTTATTGCGTCAAAAAAAAAATTGAACTTATTTTATATATTTTTTTTACAATAAACTTATTTTATATCTTAAACATTAGTGTTTCGATATACTTGTAAAATAAAAAAAAAAATTACATGATTCTATAACCTGGATTTTAAACTTATTCTAATAAATTTTGAAACTATAACTTACAAATAAGTACTTAATTAGATTTTTGTTGTTGAAAATAAATGCTTAATTAGATTCCTTAAAAAAAGTGCTTAATTAGATAAACTTATTAAATAAACCTAGTGTGTACATATGTTTAATTTTGAGACTCCATATAATTTGTCATGTCATATTATCATATCACAACAAAATAGTATATTAGGATAAATTGTTCCTTTTGTCCTTTAATTATTTTTTAGTATCACTTTGGTTTCATAATTAGTTTTTAGTCCGTTTGAGTCCTTTAAATTCATCTCCGTTATATAAGTTGGTCCCAAAAATTTAATTATGTTAATCAAAGAATTTTTTTTATCACCTTGTGCAAAAAAAAAGAATTTTTTTATCACTATGGTATAATAATTTCAAATGGATACTCAACTTTGAAAAATTGAAATATTGAACTTGGCTTGTTTGTAGATTCAATGGTGAAGATTTCTTAACAAGACTTAGAGGAAAGAGTCTCTTGTTTGTTGGAGACTCCTTGAGTTTGAATCAATGGCAATCACTCTCTTGCATGATTCACAAATCTATGCCACATATCAAGTACATCTCAGTCACCAACAAAGGTTTCTCTACCTTTACCTTCCCGGTGAGATCTTCTTCACCTACCTTTACTATTATTTAGCATATATTTTTAAAATCATTATTAAGACGATTTTAATTAACAATGTAATTTTTTCTTCAAAAAAAAAATTAACAATGTAATTTATTTCTAATTGTGTAAGTCTCAAACACTTAGTAACATAATCATTTATTTATCTTTTTGGTTTCAAGAGTTTAACGGCAAAACTAACACACACTTAAGAACTTAATGATACAAACATGTTTATCTTTTGTTTTTATCTCCATTCAATTTTTTCAAGAACTATAGTACATTTAAATTATGGTACATTTTAGAGAATAAATCATCAATGTACCAAGATAAATAAAAAATAAAAATTAAATGATGAACTGATTCTCTTTTGAAAAAATCGTGTATGGTCACAAGATTTACACACAATATTTAGACACACATTCAAAAAAAGTAAAAAAAGGTAGAAGAAATAAAAATTAAATAATGCTTATTTCTAGAATAATTAAATGATGTGTAATTCTATTTGAGGTTGTTACCAACCAAGTATTTTTTTTTTCCTTCATGACCACTTATTCCTATTATAATTTGCAGAGCTACAATGTAAAGTTGATGTTTTCCCGTAATGCATTTTTGGTGGACATTGTAAGTGAACGTATTGGGCAAGTCCTAAAATTAGATTCAATTGAAGGAGGAAGACTATGGAAAGGAAATGACATATTGATATTTAACTCTTGGCATTGGTGGCTTCACACGGGAAAAAAACAAATGTACCTAATTTTTCACTCCTCACATGCACTACTCTTAACATTACATGCATAATGCTTGATGCTTATTCCTCTTTTCTTATACTTTGCAGATGGGATTTCATTCAGGAAGGAAAACATATATACAAAGACATGAATCGTTTGGTTATGTACAAGAAAGCTTTAAAAACATGGGCGAAGTGGGTTGATGACAATGTTGATCCTAGCAAAACAAAGGTATTCTTCCAAGGAATCTCACCTGACCATAAAAAGTAAGTACATATTTTCTCCTGGATATATATTTTTTTAAGACAATTTCGTAAAAAATTTAGTCTAAGCACAAGATGCGCCAAGAATAGATCAAGAAAGTACTAAAGCGATATAACCATAAAAGTTCGAGATCAAAGTAACGACAAGGAACGACACGAAGACAAAGCACATCGCATTATCTTGTTATGTATCATTCACCCTCGAGTTAAAATCTAGTTCATCATCCAAATATAGTGAAAGATCGACAACATTGAGGAGAAGGAGGTAGAAGACTAGAAGTAATTGATTAAAATGAGAAGTAGAGTAGCATAGCAGCAGCACCTTGTGGAACCATGAACCATCTCAATTGTAAAAAAGAGACAACAGTAAGGGCAACACAAGTAATACATGAACAGAAGATTAATAACTTAAAATGTAATCCTAGTATTTATCTTATTGTTCAAGGCAATACAAAACAAAGGATAAAACTTACCAAGTATATTGGAATTTACCTAAATTATATAGAGTTATATACAACAGGGTCATCAACGTTAGAAAGGGTTTTTGTCAAAAAAAAAAAAAAAACGTTAGAAAGGGTTGAAATCCATCTTATAAGTATATTTTAAACCAATTTCAACAGTATAATTCCAACAACTTAATTCTTCATAAATCTTCTCATTCTCCAAATTATAAAATTAATTGAACAATAAGTTAATGTGCAAGTCTATATATTTGAAATTTACTTTCTTTAAATAATTATTGCAGTGGATCAAAATGGGGTCAATATGCATCTACATCTTGTAAGGGGCAAAAACTTCCATTTCCTGGATCCAAGTATTTGGGAGGTCCATACCCAGCTGAGTTTGCTTTGGAGAAAGTGATAAGAGCTATGTCAAAGCCTGTGCATTTGCTCAATATTACCACCTTATCAAAATTGAGGAAAGATGCTCACCCATCTGTATATACTGGACATGGTGGACATACCAATGACTGCAGCCATTGGTGTCTGCCTGGTGTTCCTGATACTTGGAATATGCTTTTATATGCATCTCTCATTCAAAATTAA
Protein sequence

>MS.gene027586.t1

MGFYTISGVLLLSLFIQIHGSDSFHKNGIANRGCDLFYGKWVFDDSYPLYESSECPFLENGFKCAGRPDKFYLKYRWQPNGCNFPRFNGEDFLTRLRGKSLLFVGDSLSLNQWQSLSCMIHKSMPHIKYISVTNKGFSTFTFPSYNVKLMFSRNAFLVDIVSERIGQVLKLDSIEGGRLWKGNDILIFNSWHWWLHTGKKQIWDFIQEGKHIYKDMNRLVMYKKALKTWAKWVDDNVDPSKTKVFFQGISPDHKNGSKWGQYASTSCKGQKLPFPGSKYLGGPYPAEFALEKVIRAMSKPVHLLNITTLSKLRKDAHPSVYTGHGGHTNDCSHWCLPGVPDTWNMLLYASLIQN