AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032884

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032884.t1 MTR_5g040360 96.223 609 23 0 1 609 1 609 0.0 1231
MS.gene032884.t1 MTR_8g055840 84.426 610 94 1 1 609 1 610 0.0 1101
MS.gene032884.t1 MTR_3g040370 72.559 594 159 3 15 608 17 606 0.0 937
MS.gene032884.t1 MTR_4g098890 65.630 611 193 6 8 609 9 611 0.0 865
MS.gene032884.t1 MTR_1g101370 55.814 602 239 9 18 600 25 618 0.0 695
MS.gene032884.t1 MTR_8g100075 52.818 621 262 7 9 607 17 628 0.0 690
MS.gene032884.t1 MTR_0019s0120 55.150 602 247 7 19 601 31 628 0.0 689
MS.gene032884.t1 MTR_7g076170 53.571 616 259 8 11 601 14 627 0.0 685
MS.gene032884.t1 MTR_8g432680 51.967 610 264 9 17 601 27 632 0.0 660
MS.gene032884.t1 MTR_7g006060 51.866 509 235 3 77 575 36 544 0.0 563
MS.gene032884.t1 MTR_0019s0120 53.242 509 214 8 18 507 31 534 0.0 545
MS.gene032884.t1 MTR_8g071280 46.095 525 272 7 84 601 70 590 9.47e-164 481
MS.gene032884.t1 MTR_5g026930 42.010 607 321 10 2 599 6 590 1.49e-161 475
MS.gene032884.t1 MTR_7g076150 44.280 542 273 8 71 599 62 587 2.04e-160 472
MS.gene032884.t1 MTR_8g432620 43.609 532 271 8 82 600 86 601 3.17e-159 469
MS.gene032884.t1 MTR_5g080720 43.551 535 276 11 75 596 116 637 5.90e-152 452
MS.gene032884.t1 MTR_1g069785 39.360 625 317 17 1 601 1 587 1.19e-143 429
MS.gene032884.t1 MTR_1g442840 38.654 639 346 15 1 608 1 624 2.51e-143 429
MS.gene032884.t1 MTR_3g068990 43.902 533 265 11 82 597 151 666 7.18e-143 430
MS.gene032884.t1 MTR_1g075900 42.831 551 280 12 73 601 79 616 1.37e-142 427
MS.gene032884.t1 MTR_1g029730 43.585 530 272 13 84 598 141 658 1.55e-140 423
MS.gene032884.t1 MTR_1g442840 42.913 515 264 10 111 608 10 511 7.09e-140 417
MS.gene032884.t1 MTR_7g076150 44.880 459 224 8 154 599 2 444 2.77e-138 410
MS.gene032884.t1 MTR_5g080720 43.579 475 242 11 75 536 116 577 3.05e-132 399
MS.gene032884.t1 MTR_5g026930 41.553 515 271 9 2 507 6 499 2.50e-131 394
MS.gene032884.t1 MTR_5g083150 40.000 515 274 13 92 590 100 595 1.30e-129 394
MS.gene032884.t1 MTR_5g021100 40.927 518 270 15 93 590 329 830 6.04e-125 389
MS.gene032884.t1 MTR_3g096480 40.875 526 275 13 93 599 268 776 1.75e-124 386
MS.gene032884.t1 MTR_3g071530 38.475 551 293 17 62 599 129 646 1.04e-123 380
MS.gene032884.t1 MTR_8g076650 41.426 519 265 15 93 590 387 887 4.48e-123 385
MS.gene032884.t1 MTR_1g051055 39.139 534 285 13 87 599 89 603 8.40e-123 376
MS.gene032884.t1 MTR_8g093880 40.114 526 279 13 93 599 298 806 2.53e-120 376
MS.gene032884.t1 MTR_3g088790 40.076 529 276 16 93 599 233 742 1.54e-119 372
MS.gene032884.t1 MTR_4g083030 31.216 551 338 12 70 598 72 603 6.15e-89 288
MS.gene032884.t1 MTR_3g074780 30.769 572 341 17 70 604 140 693 6.37e-88 288
MS.gene032884.t1 MTR_1g022255 32.959 534 307 15 84 599 199 699 4.64e-86 283
MS.gene032884.t1 MTR_5g064080 29.595 642 371 21 2 599 66 670 6.63e-84 276
MS.gene032884.t1 MTR_1g022255 28.500 400 241 12 84 466 199 570 1.01e-41 159
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032884.t1 AT1G26850 76.797 612 134 2 3 606 4 615 0.0 1012
MS.gene032884.t1 AT1G26850 76.797 612 134 2 3 606 4 615 0.0 1012
MS.gene032884.t1 AT4G18030 66.389 601 188 5 9 601 11 605 0.0 850
MS.gene032884.t1 AT1G26850 75.800 500 113 2 3 494 4 503 0.0 813
MS.gene032884.t1 AT4G10440 54.221 616 248 6 9 601 14 618 0.0 702
MS.gene032884.t1 AT1G33170 51.084 646 267 7 1 604 1 639 0.0 684
MS.gene032884.t1 AT4G00750 51.466 614 279 6 5 601 13 624 0.0 666
MS.gene032884.t1 AT2G45750 51.433 628 276 9 5 608 7 629 0.0 659
MS.gene032884.t1 AT2G43200 52.023 519 238 5 84 594 91 606 0.0 556
MS.gene032884.t1 AT2G43200 52.023 519 238 5 84 594 91 606 0.0 556
MS.gene032884.t1 AT1G31850 42.003 619 323 13 2 607 8 603 1.86e-168 493
MS.gene032884.t1 AT1G31850 42.003 619 323 13 2 607 8 603 1.86e-168 493
MS.gene032884.t1 AT1G31850 42.003 619 323 13 2 607 8 603 1.86e-168 493
MS.gene032884.t1 AT1G31850 42.003 619 323 13 2 607 8 603 1.86e-168 493
MS.gene032884.t1 AT4G19120 44.102 551 291 10 69 607 52 597 3.42e-166 487
MS.gene032884.t1 AT4G19120 44.102 551 291 10 69 607 52 597 3.42e-166 487
MS.gene032884.t1 AT4G00740 45.489 532 261 9 87 605 84 599 1.09e-161 476
MS.gene032884.t1 AT4G00740 45.489 532 261 9 87 605 84 599 1.09e-161 476
MS.gene032884.t1 AT5G06050 43.182 528 274 11 84 598 149 663 2.44e-145 437
MS.gene032884.t1 AT2G39750 42.056 535 284 11 78 597 167 690 1.83e-142 429
MS.gene032884.t1 AT1G04430 39.234 627 328 15 8 601 9 615 7.41e-142 426
MS.gene032884.t1 AT1G04430 39.234 627 328 15 8 601 9 615 7.41e-142 426
MS.gene032884.t1 AT1G04430 39.234 627 328 15 8 601 9 615 7.41e-142 426
MS.gene032884.t1 AT3G23300 42.620 542 276 13 81 601 76 603 2.01e-140 422
MS.gene032884.t1 AT3G23300 42.620 542 276 13 81 601 76 603 2.01e-140 422
MS.gene032884.t1 AT4G14360 43.860 513 257 11 81 575 73 572 2.62e-140 421
MS.gene032884.t1 AT4G14360 43.860 513 257 11 81 575 73 572 2.62e-140 421
MS.gene032884.t1 AT1G77260 42.056 535 282 12 76 597 135 654 4.97e-138 417
MS.gene032884.t1 AT4G19120 44.568 451 235 8 69 507 52 499 1.47e-135 405
MS.gene032884.t1 AT2G40280 41.426 519 266 13 87 590 83 578 8.58e-133 401
MS.gene032884.t1 AT2G34300 42.105 532 273 14 93 606 250 764 1.04e-131 404
MS.gene032884.t1 AT2G34300 42.105 532 273 14 93 606 250 764 1.04e-131 404
MS.gene032884.t1 AT2G34300 42.105 532 273 14 93 606 250 764 1.04e-131 404
MS.gene032884.t1 AT1G29470 41.199 534 280 12 93 609 250 766 1.64e-131 404
MS.gene032884.t1 AT1G29470 41.199 534 280 12 93 609 250 766 1.64e-131 404
MS.gene032884.t1 AT5G14430 38.537 615 325 16 11 599 17 604 7.23e-130 394
MS.gene032884.t1 AT5G04060 39.205 528 290 9 87 599 86 597 3.99e-129 392
MS.gene032884.t1 AT5G14430 38.211 615 327 16 11 599 17 604 4.94e-128 390
MS.gene032884.t1 AT3G56080 39.923 521 273 12 93 599 114 608 1.34e-127 389
MS.gene032884.t1 AT3G10200 38.649 533 288 12 87 599 75 588 2.48e-125 382
MS.gene032884.t1 AT5G64030 40.952 525 276 13 93 599 308 816 2.60e-125 389
MS.gene032884.t1 AT3G51070 41.045 536 272 19 92 604 380 894 4.14e-123 385
MS.gene032884.t1 AT3G10200 40.166 483 251 11 87 550 75 538 1.32e-116 360
MS.gene032884.t1 AT3G56080 41.333 450 232 11 93 528 114 545 1.66e-112 347
MS.gene032884.t1 AT3G51070 41.756 467 236 17 92 537 380 831 2.58e-105 337
MS.gene032884.t1 AT1G13860 32.844 545 311 14 82 593 79 601 5.93e-91 293
MS.gene032884.t1 AT1G13860 32.844 545 311 14 82 593 79 601 5.93e-91 293
MS.gene032884.t1 AT1G13860 32.844 545 311 14 82 593 79 601 5.93e-91 293
MS.gene032884.t1 AT1G13860 32.844 545 311 14 82 593 79 601 5.93e-91 293
MS.gene032884.t1 AT2G03480 30.915 634 352 17 3 598 7 592 5.49e-88 285
MS.gene032884.t1 AT2G03480 30.732 628 372 15 3 598 7 603 4.46e-87 283
MS.gene032884.t1 AT1G19430 33.142 522 304 14 93 599 231 722 3.55e-86 284
MS.gene032884.t1 AT1G19430 33.142 522 304 14 93 599 231 722 3.55e-86 284
MS.gene032884.t1 AT1G13860 35.099 453 253 12 171 593 4 445 8.99e-85 272
MS.gene032884.t1 AT1G78240 31.150 565 330 18 74 598 133 678 4.33e-84 277
MS.gene032884.t1 AT1G78240 31.150 565 330 18 74 598 133 678 4.33e-84 277
MS.gene032884.t1 AT2G03480 30.000 410 249 9 3 393 7 397 2.33e-46 169
MS.gene032884.t1 AT1G19430 29.231 390 237 11 93 468 231 595 8.00e-44 166

Find 172 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGGTTATTGGGTGCTTTC+GGG 0.168627 5.4:-30064570 MS.gene032884:CDS
TGAGAATCAAAGCTTAAATT+TGG 0.224891 5.4:+30065691 None:intergenic
CCAGTTGGGGTGCATATCTT+TGG 0.240419 5.4:-30064905 MS.gene032884:CDS
TTCTAAGACCTGGCGGTTAT+TGG 0.250522 5.4:-30064581 MS.gene032884:CDS
TGGCGGTTATTGGGTGCTTT+CGG 0.251157 5.4:-30064571 MS.gene032884:CDS
TTCTCGTTGCTTAATTCCTT+GGG 0.266033 5.4:-30064730 MS.gene032884:CDS
TGTTGTTTCTTCTACATATT+AGG 0.267388 5.4:-30065796 MS.gene032884:CDS
GTTCTACTACCAGAAGAAAT+TGG 0.274137 5.4:+30065856 None:intergenic
TGTCAGCCCCTTGAGGGAAT+TGG 0.275822 5.4:+30065320 None:intergenic
TCGTTGCAGGTATAAGAAAA+TGG 0.307086 5.4:-30064152 MS.gene032884:intron
TCCATCTGGAGCCTTTGATA+TGG 0.309219 5.4:-30064760 MS.gene032884:CDS
TGAACACATTTCCCTCATAT+TGG 0.312865 5.4:+30065362 None:intergenic
GTTCTCGTTGCTTAATTCCT+TGG 0.315484 5.4:-30064731 MS.gene032884:CDS
GACAGCAATTTCATTCTTCT+CGG 0.325154 5.4:+30064431 None:intergenic
TAACATAAATGACATTCTTC+TGG 0.335562 5.4:-30063084 MS.gene032884:CDS
GTTGCCAGTGGTTCTGTTCC+CGG 0.338306 5.4:-30064049 MS.gene032884:CDS
GTGCAGATCTTTATAGTAGT+TGG 0.347054 5.4:-30065823 MS.gene032884:CDS
GTCAGCCCCTTGAGGGAATT+GGG 0.348985 5.4:+30065321 None:intergenic
ATGGCTAAACCAATTTCTTC+TGG 0.354667 5.4:-30065865 MS.gene032884:CDS
CGGCTCTTTGGCCATGGAAA+TGG 0.358252 5.4:+30065454 None:intergenic
CTATTCAATCTCCTAAGTTA+TGG 0.359289 5.4:-30063883 MS.gene032884:CDS
GGAACATAATCACGGCTCTT+TGG 0.368346 5.4:+30065442 None:intergenic
ATAAAATTGCCTTATCCATC+TGG 0.370154 5.4:-30064774 MS.gene032884:CDS
AATTGCTGTCAAGCAGTATT+GGG 0.374007 5.4:-30062910 MS.gene032884:CDS
CGGAATATCACAGCACCTTC+AGG 0.375594 5.4:+30063041 None:intergenic
CATGGATATGAATGCTGGTT+TGG 0.377343 5.4:-30063921 MS.gene032884:CDS
CGAAGTAGACAAGAAGATTC+TGG 0.381386 5.4:-30064378 MS.gene032884:CDS
ACAGAACCACTGGCAACTTT+AGG 0.382460 5.4:+30064055 None:intergenic
GCGTGGCAGAGAACTGGTTT+TGG 0.384451 5.4:-30065772 MS.gene032884:CDS
TCTAAGACCTGGCGGTTATT+GGG 0.391716 5.4:-30064580 MS.gene032884:CDS
TTGTAGGGTGCGTTCGCATA+TGG 0.395645 5.4:+30065421 None:intergenic
AGGGAATTGGGTTCCACCAC+CGG 0.404220 5.4:+30065333 None:intergenic
TTCCACTGTAAGACTCTTGT+AGG 0.404847 5.4:+30065405 None:intergenic
AGGTTGCTAAGCTCCTTTGC+TGG 0.405835 5.4:-30064464 MS.gene032884:CDS
TATTCAATCTCCTAAGTTAT+GGG 0.408342 5.4:-30063882 MS.gene032884:CDS
ATAAATAAGCTCCTGGACTC+TGG 0.414706 5.4:-30063959 MS.gene032884:CDS
TCTTCCTCAAGATCCTCCTT+AGG 0.414914 5.4:+30064504 None:intergenic
ATCAAGGTAAAGAAAATTGT+TGG 0.416395 5.4:-30062999 MS.gene032884:CDS
TAATTAGCCTTCCAATGGAT+TGG 0.416920 5.4:+30064543 None:intergenic
CACTATGTCATTTGCACCTA+GGG 0.417543 5.4:-30064868 MS.gene032884:CDS
GGCACGCCGTTGATCATGGC+AGG 0.421898 5.4:+30065579 None:intergenic
TAATTGCTGTCAAGCAGTAT+TGG 0.427048 5.4:-30062911 MS.gene032884:CDS
AGTACAATTTGCACTTGAAA+GGG 0.428802 5.4:-30064829 MS.gene032884:CDS
ATCCTCCTTAGGTCTTTGCC+AGG 0.433073 5.4:+30064515 None:intergenic
TCAGAACTGGATCCAATATG+AGG 0.434365 5.4:-30065374 MS.gene032884:CDS
CAATTGGAGGTTACTAAGAA+AGG 0.434885 5.4:-30065733 MS.gene032884:CDS
CAAGAGTCTTACAGTGGAAA+AGG 0.435530 5.4:-30065401 MS.gene032884:CDS
ATTTATCAGCGAGGACTCAT+AGG 0.437788 5.4:-30063812 MS.gene032884:CDS
CCAAAGATATGCACCCCAAC+TGG 0.442920 5.4:+30064905 None:intergenic
GCATATAGTCTCTGTGGAAA+AGG 0.443799 5.4:+30064085 None:intergenic
CACCTTCAGGTCGTAAAATC+CGG 0.448926 5.4:+30063054 None:intergenic
TTGCAAGGCTTAAATACCTT+AGG 0.449525 5.4:+30065625 None:intergenic
CAACAGATGCTGATGATGTT+TGG 0.451762 5.4:-30064338 MS.gene032884:intron
GGCAGATGGGATTTATATGA+TGG 0.451926 5.4:-30064616 MS.gene032884:intron
GTATGTCTCAGCTGAAACTC+CGG 0.453655 5.4:+30064030 None:intergenic
TTAATTCCTTGGGGATCAAA+TGG 0.459096 5.4:-30064720 MS.gene032884:intron
TCTCCGTGGACTTTACGATT+AGG 0.459427 5.4:+30064115 None:intergenic
TTAGGTGCGTGGCAGAGAAC+TGG 0.459773 5.4:-30065778 MS.gene032884:CDS
AAGTACAATTTGCACTTGAA+AGG 0.461765 5.4:-30064830 MS.gene032884:CDS
CCTGCCGTCATTGGTGTCCT+AGG 0.462243 5.4:-30064801 MS.gene032884:CDS
ACAGCACTGGACACTGGTTG+TGG 0.462399 5.4:-30065244 MS.gene032884:CDS
TATGAGATGGGATACTAAAC+TGG 0.463047 5.4:-30062973 MS.gene032884:CDS
GGGAATTGGGTTCCACCACC+GGG 0.470402 5.4:+30065334 None:intergenic
ATGGATATGAATGCTGGTTT+GGG 0.470981 5.4:-30063920 MS.gene032884:CDS
GAGGTTACTAAGAAAGGTGT+TGG 0.471208 5.4:-30065727 MS.gene032884:CDS
TATATTTGTCAGCCCCTTGA+GGG 0.481593 5.4:+30065314 None:intergenic
TGGAAAAGGCTATTCAGAAC+TGG 0.488249 5.4:-30065387 MS.gene032884:CDS
GGTTGCTAAGCTCCTTTGCT+GGG 0.492174 5.4:-30064463 MS.gene032884:CDS
TCATAGGCATTTATCACGAT+TGG 0.492557 5.4:-30063796 MS.gene032884:intron
CAAGAAAATAAATAAGCTCC+TGG 0.495511 5.4:-30063966 MS.gene032884:CDS
TGAATGAGGTCGTACGTCCT+TGG 0.496095 5.4:+30063356 None:intergenic
CTGACTGTTCCATTCTCTAT+CGG 0.496657 5.4:+30065268 None:intergenic
GTGTTCAGATTCCCCGGTGG+TGG 0.497584 5.4:-30065346 MS.gene032884:CDS
GTAACATACCCTTTCGGTGC+TGG 0.499813 5.4:+30065478 None:intergenic
TCCACTGTAAGACTCTTGTA+GGG 0.500127 5.4:+30065406 None:intergenic
GTATGTTTCTCAGCTATAGT+AGG 0.501052 5.4:+30063845 None:intergenic
TAATCACGGCTCTTTGGCCA+TGG 0.503657 5.4:+30065448 None:intergenic
TGGAAGGTATCGCAATATCA+TGG 0.504476 5.4:-30063939 MS.gene032884:CDS
TCATGGCACGCCGTTGATCA+TGG 0.504850 5.4:+30065575 None:intergenic
ACGACCTCATTCATAGCAAT+GGG 0.505409 5.4:-30063346 MS.gene032884:CDS
TGCGATACCTTCCAGAGTCC+AGG 0.507113 5.4:+30063948 None:intergenic
GGAAGGCTAATTACAAAGCC+TGG 0.510311 5.4:-30064533 MS.gene032884:CDS
GTCGTACGTCCTTGGATATG+TGG 0.513166 5.4:+30063364 None:intergenic
TCAAAGGCTCCAGATGGATA+AGG 0.515562 5.4:+30064765 None:intergenic
CTTTGTAATTAGCCTTCCAA+TGG 0.517155 5.4:+30064538 None:intergenic
ACCAGGATGATAACGAAAAG+TGG 0.518679 5.4:-30064009 MS.gene032884:CDS
ACATTCTTCTGGAGATGGAC+CGG 0.522486 5.4:-30063073 MS.gene032884:CDS
TACGACCTCATTCATAGCAA+TGG 0.523430 5.4:-30063347 MS.gene032884:CDS
TTCTCACCATTTGATCCCCA+AGG 0.523610 5.4:+30064714 None:intergenic
TGAGGTTGATTCAAAACCTA+AGG 0.526700 5.4:-30065641 MS.gene032884:CDS
AATGGAGTAACATACCCTTT+CGG 0.527057 5.4:+30065472 None:intergenic
AATATCATGGATATGAATGC+TGG 0.527971 5.4:-30063926 MS.gene032884:CDS
CAGCACTGGACACTGGTTGT+GGG 0.532258 5.4:-30065243 MS.gene032884:CDS
CGGTCTTCTTCTCCCAGCAA+AGG 0.532439 5.4:+30064451 None:intergenic
AGAAGAATGAAATTGCTGTC+TGG 0.538296 5.4:-30064428 MS.gene032884:CDS
AGGGGTGTACCTGCCGTCAT+TGG 0.542782 5.4:-30064810 MS.gene032884:CDS
CAAAGCCTGGCAAAGACCTA+AGG 0.543305 5.4:-30064520 MS.gene032884:CDS
TTTGCACAGGTTGCCAGTTG+GGG 0.547748 5.4:-30064918 MS.gene032884:intron
TGAAGTTGATGTCTTAATCA+AGG 0.548028 5.4:-30063015 MS.gene032884:CDS
TCTGTGATACCGATAGAGAA+TGG 0.548272 5.4:-30065277 MS.gene032884:CDS
CGATAGAGAATGGAACAGTC+AGG 0.551422 5.4:-30065267 MS.gene032884:CDS
GGAAATGTGTTCAGATTCCC+CGG 0.555028 5.4:-30065352 MS.gene032884:CDS
GTTTGATACCAGCACCGAAA+GGG 0.555864 5.4:-30065486 MS.gene032884:CDS
TTAGCCTTCCAATGGATTGG+AGG 0.556029 5.4:+30064546 None:intergenic
AAATTGTTGGAGGTATGAGA+TGG 0.556242 5.4:-30062986 MS.gene032884:CDS
AAACAAGTGGACCGTCCTCA+TGG 0.559718 5.4:+30062946 None:intergenic
TCACTATGTCATTTGCACCT+AGG 0.560218 5.4:-30064869 MS.gene032884:CDS
TTTCGGGACCTCCAATCCAT+TGG 0.560894 5.4:-30064554 MS.gene032884:CDS
ACATTCATGACCCATAACTT+AGG 0.560995 5.4:+30063872 None:intergenic
TGTGCTTCATGTGAGTCCCT+AGG 0.562927 5.4:+30064852 None:intergenic
AATTGTTGGAGGTATGAGAT+GGG 0.562974 5.4:-30062985 MS.gene032884:CDS
AAATGACATTCTTCTGGAGA+TGG 0.564104 5.4:-30063078 MS.gene032884:CDS
GAACCACTGGCAACTTTAGG+AGG 0.564388 5.4:+30064058 None:intergenic
TGGAACAGTCAGGACAGCAC+TGG 0.564499 5.4:-30065257 MS.gene032884:CDS
AGAACAAAGAAAGATTGAAG+AGG 0.565209 5.4:-30064484 MS.gene032884:CDS
CAGAACTGGATCCAATATGA+GGG 0.569553 5.4:-30065373 MS.gene032884:CDS
TCGATATATCGAGCCTTGCA+AGG 0.569809 5.4:+30065610 None:intergenic
AGGTTACTAAGAAAGGTGTT+GGG 0.570211 5.4:-30065726 MS.gene032884:CDS
AACTCCGGGAACAGAACCAC+TGG 0.570803 5.4:+30064045 None:intergenic
GTCAGGACAGCACTGGACAC+TGG 0.572331 5.4:-30065250 MS.gene032884:CDS
AAGGTAAAGAAAATTGTTGG+AGG 0.572644 5.4:-30062996 MS.gene032884:CDS
GGGACCTCCAATCCATTGGA+AGG 0.573733 5.4:-30064550 MS.gene032884:CDS
TAAGGTATTTAAGCCTTGCA+AGG 0.576659 5.4:-30065623 MS.gene032884:CDS
TATGTCTCAGCTGAAACTCC+GGG 0.580149 5.4:+30064031 None:intergenic
ATAAGCTCCTGGACTCTGGA+AGG 0.581133 5.4:-30063955 MS.gene032884:CDS
AGAACAATGTGCCATATCAA+AGG 0.583862 5.4:+30064749 None:intergenic
GTGGAACCCAATTCCCTCAA+GGG 0.584099 5.4:-30065327 MS.gene032884:CDS
AACCGCCAGGTCTTAGAACT+CGG 0.584438 5.4:+30064586 None:intergenic
GAAAGCACCCAATAACCGCC+AGG 0.587918 5.4:+30064573 None:intergenic
GGTGGAACCCAATTCCCTCA+AGG 0.590459 5.4:-30065328 MS.gene032884:CDS
AAAATATGATTTATCGAGAG+AGG 0.590909 5.4:-30065540 MS.gene032884:CDS
GCCATATCAAAGGCTCCAGA+TGG 0.590985 5.4:+30064759 None:intergenic
TTAAGCTTTGATTCTCACCA+TGG 0.593567 5.4:-30065685 MS.gene032884:CDS
AAGACCTAAGGAGGATCTTG+AGG 0.593947 5.4:-30064508 MS.gene032884:CDS
AGCACTGGACACTGGTTGTG+GGG 0.594228 5.4:-30065242 MS.gene032884:intron
ATATATTTGTCAGCCCCTTG+AGG 0.597173 5.4:+30065313 None:intergenic
ACACACCCTGCCATGATCAA+CGG 0.601047 5.4:-30065585 MS.gene032884:CDS
GGTATGTTACTCCATTTCCA+TGG 0.603095 5.4:-30065465 MS.gene032884:CDS
AGAAGTTAGCAAAATTGATG+AGG 0.606974 5.4:-30065659 MS.gene032884:CDS
TCTCGTTGCTTAATTCCTTG+GGG 0.606987 5.4:-30064729 MS.gene032884:CDS
CGTTTGATACCAGCACCGAA+AGG 0.611026 5.4:-30065487 MS.gene032884:CDS
AGTTTCAGCTGAGACATACC+AGG 0.611606 5.4:-30064026 MS.gene032884:CDS
GTTCCTCCTAAAGTTGCCAG+TGG 0.612508 5.4:-30064061 MS.gene032884:CDS
CCTAGGACACCAATGACGGC+AGG 0.614190 5.4:+30064801 None:intergenic
TACTAAACTGGTTGACCATG+AGG 0.614521 5.4:-30062961 MS.gene032884:CDS
TTTACGATTAGGAGTAACAC+AGG 0.615416 5.4:+30064126 None:intergenic
AGCTTTGATTCTCACCATGG+TGG 0.615893 5.4:-30065682 MS.gene032884:CDS
GCACGCCGTTGATCATGGCA+GGG 0.616382 5.4:+30065580 None:intergenic
TGGGCTCTTTAGTCTGTACA+AGG 0.617149 5.4:-30063327 MS.gene032884:CDS
AGAGCCCATTGCTATGAATG+AGG 0.631489 5.4:+30063342 None:intergenic
GGTTCCTAGGACACCAATGA+CGG 0.632608 5.4:+30064797 None:intergenic
AAGCTTTCTCCACATATCCA+AGG 0.633136 5.4:-30063373 MS.gene032884:CDS
AGCCTGGCAAAGACCTAAGG+AGG 0.634638 5.4:-30064517 MS.gene032884:CDS
GTTGACCGAGTTCTAAGACC+TGG 0.636678 5.4:-30064591 MS.gene032884:CDS
GTACAATTTGCACTTGAAAG+GGG 0.638641 5.4:-30064828 MS.gene032884:CDS
TCTTCTACATATTAGGTGCG+TGG 0.640208 5.4:-30065789 MS.gene032884:CDS
GAGTGGTTATTGTGCTGACG+TGG 0.643693 5.4:+30062872 None:intergenic
TGGAACCCAATTCCCTCAAG+GGG 0.646344 5.4:-30065326 MS.gene032884:CDS
GGAACTGCATATAGTCTCTG+TGG 0.648475 5.4:+30064079 None:intergenic
ACTCCTAATCGTAAAGTCCA+CGG 0.651226 5.4:-30064118 MS.gene032884:CDS
AAACTGGTTGACCATGAGGA+CGG 0.653450 5.4:-30062957 MS.gene032884:CDS
TGTAACTTCTCTTCTTCACG+AGG 0.654884 5.4:+30065511 None:intergenic
TCGCATATGGAACATAATCA+CGG 0.658822 5.4:+30065434 None:intergenic
GACCGAGTTCTAAGACCTGG+CGG 0.681581 5.4:-30064588 MS.gene032884:CDS
TTAAGACATCAACTTCATCA+CGG 0.685496 5.4:+30063021 None:intergenic
CTCGGTGTCATTTATCAGCG+AGG 0.696680 5.4:-30063821 MS.gene032884:CDS
GTAACTTCTCTTCTTCACGA+GGG 0.701314 5.4:+30065512 None:intergenic
GAAAAGGCTTAAGATCTCCG+TGG 0.706045 5.4:+30064101 None:intergenic
ATAGCTGAGAAACATACACT+CGG 0.706522 5.4:-30063839 MS.gene032884:CDS
GGAATTGGGTTCCACCACCG+GGG 0.720073 5.4:+30065335 None:intergenic
ACCCTACAAGAGTCTTACAG+TGG 0.737297 5.4:-30065407 MS.gene032884:CDS
AATGTGTTCAGATTCCCCGG+TGG 0.776641 5.4:-30065349 MS.gene032884:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAATAACCATATAATTAA+AGG + chr5.4:30065455-30065474 None:intergenic 15.0%
!! TGAGTATTCCTTTAATTATA+TGG - chr5.4:30065444-30065463 MS.gene032884:CDS 20.0%
!! TGTTAGTAGAACTAAATTAA+TGG + chr5.4:30064488-30064507 None:intergenic 20.0%
!!! AATCATATTTTGTCTAGAAA+AGG + chr5.4:30063194-30063213 None:intergenic 20.0%
!!! CATAAAGGCATAAATAAATA+AGG + chr5.4:30065339-30065358 None:intergenic 20.0%
! AAAATATGATTTATCGAGAG+AGG - chr5.4:30063203-30063222 MS.gene032884:intron 25.0%
! AATTAATGGAATACCAACAA+TGG + chr5.4:30064474-30064493 None:intergenic 25.0%
! ACATAAAAAGTAAGAGAAAG+AGG - chr5.4:30065147-30065166 MS.gene032884:intron 25.0%
! AGGTTAGAGATAAACATATA+CGG - chr5.4:30065167-30065186 MS.gene032884:intron 25.0%
! ATCAAGGTAAAGAAAATTGT+TGG - chr5.4:30065744-30065763 MS.gene032884:CDS 25.0%
! GAATGCATTATAAGTTGTAT+AGG - chr5.4:30065504-30065523 MS.gene032884:CDS 25.0%
! TAACATAAATGACATTCTTC+TGG - chr5.4:30065659-30065678 MS.gene032884:CDS 25.0%
! TATTCAATCTCCTAAGTTAT+GGG - chr5.4:30064861-30064880 MS.gene032884:CDS 25.0%
! TGAGAATCAAAGCTTAAATT+TGG + chr5.4:30063055-30063074 None:intergenic 25.0%
! TTATAATGCATTCTATGCAA+AGG + chr5.4:30065497-30065516 None:intergenic 25.0%
!!! TGTTGTTTCTTCTACATATT+AGG - chr5.4:30062947-30062966 MS.gene032884:CDS 25.0%
AAGAGAGAACTAAAACTAAG+TGG + chr5.4:30065573-30065592 None:intergenic 30.0%
AAGGTAAAGAAAATTGTTGG+AGG - chr5.4:30065747-30065766 MS.gene032884:CDS 30.0%
AAGTACAATTTGCACTTGAA+AGG - chr5.4:30063913-30063932 MS.gene032884:CDS 30.0%
AATATCATGGATATGAATGC+TGG - chr5.4:30064817-30064836 MS.gene032884:CDS 30.0%
ACAAAAATCTTGTACTGAGT+TGG - chr5.4:30064553-30064572 MS.gene032884:CDS 30.0%
AGAACAAAGAAAGATTGAAG+AGG - chr5.4:30064259-30064278 MS.gene032884:intron 30.0%
AGAAGTTAGCAAAATTGATG+AGG - chr5.4:30063084-30063103 MS.gene032884:CDS 30.0%
AGTACAATTTGCACTTGAAA+GGG - chr5.4:30063914-30063933 MS.gene032884:CDS 30.0%
ATAAAATTGCCTTATCCATC+TGG - chr5.4:30063969-30063988 MS.gene032884:CDS 30.0%
CAAGAAAATAAATAAGCTCC+TGG - chr5.4:30064777-30064796 MS.gene032884:CDS 30.0%
CTATTCAATCTCCTAAGTTA+TGG - chr5.4:30064860-30064879 MS.gene032884:CDS 30.0%
GACACTCTGAATAAAATAGA+AGG + chr5.4:30064992-30065011 None:intergenic 30.0%
TATTTATGCCTTTATGATCC+AGG - chr5.4:30065343-30065362 MS.gene032884:CDS 30.0%
TGCATTATAAGTTGTATAGG+TGG - chr5.4:30065507-30065526 MS.gene032884:CDS 30.0%
TTAAGACATCAACTTCATCA+CGG + chr5.4:30065725-30065744 None:intergenic 30.0%
TTAGCACAACTTTCTTTGAT+CGG - chr5.4:30065289-30065308 MS.gene032884:CDS 30.0%
! AAAGGGAGATCTTTTACAAT+TGG - chr5.4:30062994-30063013 MS.gene032884:CDS 30.0%
! AAATAACGAAAGTGCATCTT+GGG - chr5.4:30064096-30064115 MS.gene032884:CDS 30.0%
! AGATGGATAAGGCAATTTTA+TGG + chr5.4:30063970-30063989 None:intergenic 30.0%
! AGTATCTTTTCAGAAACAAG+TGG + chr5.4:30065813-30065832 None:intergenic 30.0%
! ATTTTGTCTAGAAAAGGTCA+TGG + chr5.4:30063188-30063207 None:intergenic 30.0%
! TAAATAACGAAAGTGCATCT+TGG - chr5.4:30064095-30064114 MS.gene032884:CDS 30.0%
! TGCATTTGATGATTAGATAC+AGG + chr5.4:30065635-30065654 None:intergenic 30.0%
!! TGAAGTTGATGTCTTAATCA+AGG - chr5.4:30065728-30065747 MS.gene032884:CDS 30.0%
!! TTCAAAGTGTTGTCAAATAG+AGG - chr5.4:30063693-30063712 MS.gene032884:intron 30.0%
!!! TGAGTTAGTTTGTTTTAGCT+AGG - chr5.4:30063780-30063799 MS.gene032884:intron 30.0%
AAGGAAAAACATGCGAGAAA+AGG + chr5.4:30065320-30065339 None:intergenic 35.0%
AATGGAGTAACATACCCTTT+CGG + chr5.4:30063274-30063293 None:intergenic 35.0%
AATTGCTGTCAAGCAGTATT+GGG - chr5.4:30065833-30065852 MS.gene032884:CDS 35.0%
ACATTCATGACCCATAACTT+AGG + chr5.4:30064874-30064893 None:intergenic 35.0%
AGAACAATGTGCCATATCAA+AGG + chr5.4:30063997-30064016 None:intergenic 35.0%
AGAAGAATGAAATTGCTGTC+TGG - chr5.4:30064315-30064334 MS.gene032884:intron 35.0%
AGAATATGATACAAGCCCTA+AGG + chr5.4:30063547-30063566 None:intergenic 35.0%
AGGAAAAACATGCGAGAAAA+GGG + chr5.4:30065319-30065338 None:intergenic 35.0%
ATAGCTGAGAAACATACACT+CGG - chr5.4:30064904-30064923 MS.gene032884:CDS 35.0%
ATGGCTAAACCAATTTCTTC+TGG - chr5.4:30062878-30062897 MS.gene032884:CDS 35.0%
CAAATACTGCTATGCTATAG+CGG + chr5.4:30063638-30063657 None:intergenic 35.0%
CAATTGGAGGTTACTAAGAA+AGG - chr5.4:30063010-30063029 MS.gene032884:CDS 35.0%
CTAGGTATCTGTAATGTGTA+AGG - chr5.4:30063798-30063817 MS.gene032884:CDS 35.0%
GACAGCAATTTCATTCTTCT+CGG + chr5.4:30064315-30064334 None:intergenic 35.0%
GTACAATTTGCACTTGAAAG+GGG - chr5.4:30063915-30063934 MS.gene032884:CDS 35.0%
GTATGTTTCTCAGCTATAGT+AGG + chr5.4:30064901-30064920 None:intergenic 35.0%
GTGCAGATCTTTATAGTAGT+TGG - chr5.4:30062920-30062939 MS.gene032884:CDS 35.0%
GTTCTACTACCAGAAGAAAT+TGG + chr5.4:30062890-30062909 None:intergenic 35.0%
TAATTGCTGTCAAGCAGTAT+TGG - chr5.4:30065832-30065851 MS.gene032884:CDS 35.0%
TAGCACAACTTTCTTTGATC+GGG - chr5.4:30065290-30065309 MS.gene032884:CDS 35.0%
TATGAGATGGGATACTAAAC+TGG - chr5.4:30065770-30065789 MS.gene032884:CDS 35.0%
TCATAGGCATTTATCACGAT+TGG - chr5.4:30064947-30064966 MS.gene032884:intron 35.0%
TCGCATATGGAACATAATCA+CGG + chr5.4:30063312-30063331 None:intergenic 35.0%
TCGTTGCAGGTATAAGAAAA+TGG - chr5.4:30064591-30064610 MS.gene032884:CDS 35.0%
TGAACACATTTCCCTCATAT+TGG + chr5.4:30063384-30063403 None:intergenic 35.0%
TTAATTCCTTGGGGATCAAA+TGG - chr5.4:30064023-30064042 MS.gene032884:CDS 35.0%
TTCTCGTTGCTTAATTCCTT+GGG - chr5.4:30064013-30064032 MS.gene032884:CDS 35.0%
TTGCAAGGCTTAAATACCTT+AGG + chr5.4:30063121-30063140 None:intergenic 35.0%
TTTACGATTAGGAGTAACAC+AGG + chr5.4:30064620-30064639 None:intergenic 35.0%
! AAATGACATTCTTCTGGAGA+TGG - chr5.4:30065665-30065684 MS.gene032884:CDS 35.0%
! AAATTGTTGGAGGTATGAGA+TGG - chr5.4:30065757-30065776 MS.gene032884:CDS 35.0%
! AAGGCAATTTTATGGTTCCT+AGG + chr5.4:30063962-30063981 None:intergenic 35.0%
! AATCTTGTACTGAGTTGGAA+TGG - chr5.4:30064558-30064577 MS.gene032884:CDS 35.0%
! AATTGTTGGAGGTATGAGAT+GGG - chr5.4:30065758-30065777 MS.gene032884:CDS 35.0%
! CGTTTTCATGCAATACATCA+AGG + chr5.4:30065250-30065269 None:intergenic 35.0%
! TAAGGTATTTAAGCCTTGCA+AGG - chr5.4:30063120-30063139 MS.gene032884:intron 35.0%
! TAGCAGCATAGTCTAGTTTA+GGG - chr5.4:30063593-30063612 MS.gene032884:intron 35.0%
! TGAGGTTGATTCAAAACCTA+AGG - chr5.4:30063102-30063121 MS.gene032884:intron 35.0%
! TTAAGCTTTGATTCTCACCA+TGG - chr5.4:30063058-30063077 MS.gene032884:CDS 35.0%
! TTAGCAGCATAGTCTAGTTT+AGG - chr5.4:30063592-30063611 MS.gene032884:intron 35.0%
!! AACATATACGGTGTCACATA+TGG - chr5.4:30065179-30065198 MS.gene032884:intron 35.0%
!! AGGTTACTAAGAAAGGTGTT+GGG - chr5.4:30063017-30063036 MS.gene032884:CDS 35.0%
!! ATGGATATGAATGCTGGTTT+GGG - chr5.4:30064823-30064842 MS.gene032884:CDS 35.0%
!! CTTTGTAATTAGCCTTCCAA+TGG + chr5.4:30064208-30064227 None:intergenic 35.0%
!! TAATTAGCCTTCCAATGGAT+TGG + chr5.4:30064203-30064222 None:intergenic 35.0%
!! TGCATTTTAGCATCCATTGT+TGG - chr5.4:30064458-30064477 MS.gene032884:CDS 35.0%
AAGCTTTCTCCACATATCCA+AGG - chr5.4:30065370-30065389 MS.gene032884:CDS 40.0%
AATGAACACAACTGGCAAAG+GGG + chr5.4:30065597-30065616 None:intergenic 40.0%
ACGACCTCATTCATAGCAAT+GGG - chr5.4:30065397-30065416 MS.gene032884:CDS 40.0%
ACTCCTAATCGTAAAGTCCA+CGG - chr5.4:30064625-30064644 MS.gene032884:intron 40.0%
AGAATGAACACAACTGGCAA+AGG + chr5.4:30065599-30065618 None:intergenic 40.0%
ATAAATAAGCTCCTGGACTC+TGG - chr5.4:30064784-30064803 MS.gene032884:CDS 40.0%
ATATATTTGTCAGCCCCTTG+AGG + chr5.4:30063433-30063452 None:intergenic 40.0%
ATTTATCAGCGAGGACTCAT+AGG - chr5.4:30064931-30064950 MS.gene032884:intron 40.0%
CAAGAGTCTTACAGTGGAAA+AGG - chr5.4:30063342-30063361 MS.gene032884:CDS 40.0%
CAGAACTGGATCCAATATGA+GGG - chr5.4:30063370-30063389 MS.gene032884:CDS 40.0%
CGAAGTAGACAAGAAGATTC+TGG - chr5.4:30064365-30064384 MS.gene032884:CDS 40.0%
CTGACTGTTCCATTCTCTAT+CGG + chr5.4:30063478-30063497 None:intergenic 40.0%
GAATGAACACAACTGGCAAA+GGG + chr5.4:30065598-30065617 None:intergenic 40.0%
GACACAAGAATGAACACAAC+TGG + chr5.4:30065605-30065624 None:intergenic 40.0%
GCATATAGTCTCTGTGGAAA+AGG + chr5.4:30064661-30064680 None:intergenic 40.0%
GGCAGATGGGATTTATATGA+TGG - chr5.4:30064127-30064146 MS.gene032884:CDS 40.0%
GGTATGTTACTCCATTTCCA+TGG - chr5.4:30063278-30063297 MS.gene032884:intron 40.0%
GTAACTTCTCTTCTTCACGA+GGG + chr5.4:30063234-30063253 None:intergenic 40.0%
GTTCTCGTTGCTTAATTCCT+TGG - chr5.4:30064012-30064031 MS.gene032884:CDS 40.0%
GTTGTGCTAACAATACACGA+CGG + chr5.4:30065279-30065298 None:intergenic 40.0%
TAACTCAGTGATGTTAGTCG+CGG + chr5.4:30063767-30063786 None:intergenic 40.0%
TACGACCTCATTCATAGCAA+TGG - chr5.4:30065396-30065415 MS.gene032884:CDS 40.0%
TACTAAACTGGTTGACCATG+AGG - chr5.4:30065782-30065801 MS.gene032884:CDS 40.0%
TATATTTGTCAGCCCCTTGA+GGG + chr5.4:30063432-30063451 None:intergenic 40.0%
TCAGAACTGGATCCAATATG+AGG - chr5.4:30063369-30063388 MS.gene032884:CDS 40.0%
TCCACTGTAAGACTCTTGTA+GGG + chr5.4:30063340-30063359 None:intergenic 40.0%
TCTCGTTGCTTAATTCCTTG+GGG - chr5.4:30064014-30064033 MS.gene032884:CDS 40.0%
TCTGTGATACCGATAGAGAA+TGG - chr5.4:30063466-30063485 MS.gene032884:intron 40.0%
TCTTCTACATATTAGGTGCG+TGG - chr5.4:30062954-30062973 MS.gene032884:CDS 40.0%
TGGAAAAGGCTATTCAGAAC+TGG - chr5.4:30063356-30063375 MS.gene032884:CDS 40.0%
TGTAACTTCTCTTCTTCACG+AGG + chr5.4:30063235-30063254 None:intergenic 40.0%
TTCCACTGTAAGACTCTTGT+AGG + chr5.4:30063341-30063360 None:intergenic 40.0%
! ACCAGGATGATAACGAAAAG+TGG - chr5.4:30064734-30064753 MS.gene032884:CDS 40.0%
! AGTGCATCTTGGGTTGTTAT+TGG - chr5.4:30064106-30064125 MS.gene032884:CDS 40.0%
! CAACAGATGCTGATGATGTT+TGG - chr5.4:30064405-30064424 MS.gene032884:CDS 40.0%
! CACTATGTCATTTGCACCTA+GGG - chr5.4:30063875-30063894 MS.gene032884:CDS 40.0%
! CATGGATATGAATGCTGGTT+TGG - chr5.4:30064822-30064841 MS.gene032884:CDS 40.0%
! GAGGTTACTAAGAAAGGTGT+TGG - chr5.4:30063016-30063035 MS.gene032884:CDS 40.0%
! GGGAGATCTTTTACAATTGG+AGG - chr5.4:30062997-30063016 MS.gene032884:CDS 40.0%
! TCACTATGTCATTTGCACCT+AGG - chr5.4:30063874-30063893 MS.gene032884:CDS 40.0%
! TTGGGTTGTTATTGGCAGAT+GGG - chr5.4:30064114-30064133 MS.gene032884:CDS 40.0%
! TTTTGCTAACTTCTCCACCA+TGG + chr5.4:30063078-30063097 None:intergenic 40.0%
!! TCCACTTTTCGTTATCATCC+TGG + chr5.4:30064738-30064757 None:intergenic 40.0%
!! TGGAAGGTATCGCAATATCA+TGG - chr5.4:30064804-30064823 MS.gene032884:CDS 40.0%
!! TGTGTAAGGTTGTTTTGCAC+AGG - chr5.4:30063812-30063831 MS.gene032884:CDS 40.0%
!!! CAGAGAACTGGTTTTGGAAA+GGG - chr5.4:30062977-30062996 MS.gene032884:CDS 40.0%
!!! TGGATTTGTTTTGTCGTTGC+AGG - chr5.4:30064578-30064597 MS.gene032884:CDS 40.0%
AAACTGGTTGACCATGAGGA+CGG - chr5.4:30065786-30065805 MS.gene032884:CDS 45.0%
AAGACCTAAGGAGGATCTTG+AGG - chr5.4:30064235-30064254 MS.gene032884:intron 45.0%
ACAGAACCACTGGCAACTTT+AGG + chr5.4:30064691-30064710 None:intergenic 45.0%
ACCCTACAAGAGTCTTACAG+TGG - chr5.4:30063336-30063355 MS.gene032884:CDS 45.0%
AGAGCCCATTGCTATGAATG+AGG + chr5.4:30065404-30065423 None:intergenic 45.0%
AGTTTCAGCTGAGACATACC+AGG - chr5.4:30064717-30064736 MS.gene032884:intron 45.0%
CACAACTTTCTTTGATCGGG+TGG - chr5.4:30065293-30065312 MS.gene032884:CDS 45.0%
CACCTTCAGGTCGTAAAATC+CGG + chr5.4:30065692-30065711 None:intergenic 45.0%
CGATAGAGAATGGAACAGTC+AGG - chr5.4:30063476-30063495 MS.gene032884:intron 45.0%
GAAAAGGCTTAAGATCTCCG+TGG + chr5.4:30064645-30064664 None:intergenic 45.0%
GCTTCACACCTGGATCATAA+AGG + chr5.4:30065354-30065373 None:intergenic 45.0%
GGAAATGTGTTCAGATTCCC+CGG - chr5.4:30063391-30063410 MS.gene032884:intron 45.0%
GGAACATAATCACGGCTCTT+TGG + chr5.4:30063304-30063323 None:intergenic 45.0%
GGAACTGCATATAGTCTCTG+TGG + chr5.4:30064667-30064686 None:intergenic 45.0%
GGAAGGCTAATTACAAAGCC+TGG - chr5.4:30064210-30064229 MS.gene032884:intron 45.0%
GTATGTCTCAGCTGAAACTC+CGG + chr5.4:30064716-30064735 None:intergenic 45.0%
TATGTCTCAGCTGAAACTCC+GGG + chr5.4:30064715-30064734 None:intergenic 45.0%
TCAAAGGCTCCAGATGGATA+AGG + chr5.4:30063981-30064000 None:intergenic 45.0%
TCGATATATCGAGCCTTGCA+AGG + chr5.4:30063136-30063155 None:intergenic 45.0%
TCTCCGTGGACTTTACGATT+AGG + chr5.4:30064631-30064650 None:intergenic 45.0%
TCTTCCTCAAGATCCTCCTT+AGG + chr5.4:30064242-30064261 None:intergenic 45.0%
TGGGCTCTTTAGTCTGTACA+AGG - chr5.4:30065416-30065435 MS.gene032884:CDS 45.0%
TTCTCACCATTTGATCCCCA+AGG + chr5.4:30064032-30064051 None:intergenic 45.0%
TTGCCTGCGTGAATTCCTTA+GGG - chr5.4:30063529-30063548 MS.gene032884:intron 45.0%
TTTGCCTGCGTGAATTCCTT+AGG - chr5.4:30063528-30063547 MS.gene032884:intron 45.0%
! ACATTCTTCTGGAGATGGAC+CGG - chr5.4:30065670-30065689 MS.gene032884:CDS 45.0%
! AGCTTTGATTCTCACCATGG+TGG - chr5.4:30063061-30063080 MS.gene032884:CDS 45.0%
! CTTGGGTTGTTATTGGCAGA+TGG - chr5.4:30064113-30064132 MS.gene032884:CDS 45.0%
! GTGATGTTAGTCGCGGATTA+TGG + chr5.4:30063760-30063779 None:intergenic 45.0%
! GTTTGATACCAGCACCGAAA+GGG - chr5.4:30063257-30063276 MS.gene032884:intron 45.0%
! TCCATCTGGAGCCTTTGATA+TGG - chr5.4:30063983-30064002 MS.gene032884:CDS 45.0%
! TCTAAGACCTGGCGGTTATT+GGG - chr5.4:30064163-30064182 MS.gene032884:intron 45.0%
! TTTTGCACAGGTTGCCAGTT+GGG - chr5.4:30063824-30063843 MS.gene032884:CDS 45.0%
!! TTAGCCTTCCAATGGATTGG+AGG + chr5.4:30064200-30064219 None:intergenic 45.0%
!! TTCTAAGACCTGGCGGTTAT+TGG - chr5.4:30064162-30064181 MS.gene032884:intron 45.0%
!!! GCAGAGAACTGGTTTTGGAA+AGG - chr5.4:30062976-30062995 MS.gene032884:CDS 45.0%
AAACAAGTGGACCGTCCTCA+TGG + chr5.4:30065800-30065819 None:intergenic 50.0%
AACCGCCAGGTCTTAGAACT+CGG + chr5.4:30064160-30064179 None:intergenic 50.0%
AAGCCCTAAGGAATTCACGC+AGG + chr5.4:30063535-30063554 None:intergenic 50.0%
AATGTGTTCAGATTCCCCGG+TGG - chr5.4:30063394-30063413 MS.gene032884:intron 50.0%
ACACACCCTGCCATGATCAA+CGG - chr5.4:30063158-30063177 MS.gene032884:intron 50.0%
AGGTTGCTAAGCTCCTTTGC+TGG - chr5.4:30064279-30064298 MS.gene032884:intron 50.0%
ATAAGCTCCTGGACTCTGGA+AGG - chr5.4:30064788-30064807 MS.gene032884:CDS 50.0%
ATCCTCCTTAGGTCTTTGCC+AGG + chr5.4:30064231-30064250 None:intergenic 50.0%
CAAAGCCTGGCAAAGACCTA+AGG - chr5.4:30064223-30064242 MS.gene032884:intron 50.0%
CCAAAGATATGCACCCCAAC+TGG + chr5.4:30063841-30063860 None:intergenic 50.0%
CCAGTTGGGGTGCATATCTT+TGG - chr5.4:30063838-30063857 MS.gene032884:CDS 50.0%
CGGAATATCACAGCACCTTC+AGG + chr5.4:30065705-30065724 None:intergenic 50.0%
GAACCACTGGCAACTTTAGG+AGG + chr5.4:30064688-30064707 None:intergenic 50.0%
GCCATATCAAAGGCTCCAGA+TGG + chr5.4:30063987-30064006 None:intergenic 50.0%
GGTTCCTAGGACACCAATGA+CGG + chr5.4:30063949-30063968 None:intergenic 50.0%
GGTTGCTAAGCTCCTTTGCT+GGG - chr5.4:30064280-30064299 MS.gene032884:intron 50.0%
GTCGTACGTCCTTGGATATG+TGG + chr5.4:30065382-30065401 None:intergenic 50.0%
GTGGAACCCAATTCCCTCAA+GGG - chr5.4:30063416-30063435 MS.gene032884:intron 50.0%
GTTCCTCCTAAAGTTGCCAG+TGG - chr5.4:30064682-30064701 MS.gene032884:intron 50.0%
TAATCACGGCTCTTTGGCCA+TGG + chr5.4:30063298-30063317 None:intergenic 50.0%
TGAATGAGGTCGTACGTCCT+TGG + chr5.4:30065390-30065409 None:intergenic 50.0%
TGGAACCCAATTCCCTCAAG+GGG - chr5.4:30063417-30063436 MS.gene032884:intron 50.0%
TGGCGGTTATTGGGTGCTTT+CGG - chr5.4:30064172-30064191 MS.gene032884:intron 50.0%
TGTGCTTCATGTGAGTCCCT+AGG + chr5.4:30063894-30063913 None:intergenic 50.0%
TGTGGAGAAAGCTTCACACC+TGG + chr5.4:30065364-30065383 None:intergenic 50.0%
TTGTAGGGTGCGTTCGCATA+TGG + chr5.4:30063325-30063344 None:intergenic 50.0%
TTTCGGGACCTCCAATCCAT+TGG - chr5.4:30064189-30064208 MS.gene032884:intron 50.0%
TTTGCACAGGTTGCCAGTTG+GGG - chr5.4:30063825-30063844 MS.gene032884:CDS 50.0%
! CGTTTGATACCAGCACCGAA+AGG - chr5.4:30063256-30063275 MS.gene032884:intron 50.0%
! GACCGGATTTTACGACCTGA+AGG - chr5.4:30065687-30065706 MS.gene032884:CDS 50.0%
! GTAACATACCCTTTCGGTGC+TGG + chr5.4:30063268-30063287 None:intergenic 50.0%
! GTTTTGCACAGGTTGCCAGT+TGG - chr5.4:30063823-30063842 MS.gene032884:CDS 50.0%
!! CTCGGTGTCATTTATCAGCG+AGG - chr5.4:30064922-30064941 MS.gene032884:intron 50.0%
!! GTTGACCGAGTTCTAAGACC+TGG - chr5.4:30064152-30064171 MS.gene032884:intron 50.0%
AACTCCGGGAACAGAACCAC+TGG + chr5.4:30064701-30064720 None:intergenic 55.0%
AGCCTGGCAAAGACCTAAGG+AGG - chr5.4:30064226-30064245 MS.gene032884:intron 55.0%
AGGGAATTGGGTTCCACCAC+CGG + chr5.4:30063413-30063432 None:intergenic 55.0%
CGGCTCTTTGGCCATGGAAA+TGG + chr5.4:30063292-30063311 None:intergenic 55.0%
CGGTCTTCTTCTCCCAGCAA+AGG + chr5.4:30064295-30064314 None:intergenic 55.0%
GGCGGTTATTGGGTGCTTTC+GGG - chr5.4:30064173-30064192 MS.gene032884:intron 55.0%
GGGACCTCCAATCCATTGGA+AGG - chr5.4:30064193-30064212 MS.gene032884:intron 55.0%
GGTGGAACCCAATTCCCTCA+AGG - chr5.4:30063415-30063434 MS.gene032884:intron 55.0%
GTCAGCCCCTTGAGGGAATT+GGG + chr5.4:30063425-30063444 None:intergenic 55.0%
TCATGGCACGCCGTTGATCA+TGG + chr5.4:30063171-30063190 None:intergenic 55.0%
TGCGATACCTTCCAGAGTCC+AGG + chr5.4:30064798-30064817 None:intergenic 55.0%
TGGAACAGTCAGGACAGCAC+TGG - chr5.4:30063486-30063505 MS.gene032884:intron 55.0%
TGTCAGCCCCTTGAGGGAAT+TGG + chr5.4:30063426-30063445 None:intergenic 55.0%
TTAGGTGCGTGGCAGAGAAC+TGG - chr5.4:30062965-30062984 MS.gene032884:CDS 55.0%
! ACAGCACTGGACACTGGTTG+TGG - chr5.4:30063499-30063518 MS.gene032884:intron 55.0%
! AGCACTGGACACTGGTTGTG+GGG - chr5.4:30063501-30063520 MS.gene032884:intron 55.0%
! CAGCACTGGACACTGGTTGT+GGG - chr5.4:30063500-30063519 MS.gene032884:intron 55.0%
! GAGGCGCTATAGCACTGTAG+TGG - chr5.4:30063712-30063731 MS.gene032884:intron 55.0%
! GTTGCCAGTGGTTCTGTTCC+CGG - chr5.4:30064694-30064713 MS.gene032884:intron 55.0%
!! GAAAGCACCCAATAACCGCC+AGG + chr5.4:30064173-30064192 None:intergenic 55.0%
!! GACCGAGTTCTAAGACCTGG+CGG - chr5.4:30064155-30064174 MS.gene032884:intron 55.0%
!! GCGTGGCAGAGAACTGGTTT+TGG - chr5.4:30062971-30062990 MS.gene032884:CDS 55.0%
CCTAGGACACCAATGACGGC+AGG + chr5.4:30063945-30063964 None:intergenic 60.0%
GCACGCCGTTGATCATGGCA+GGG + chr5.4:30063166-30063185 None:intergenic 60.0%
GGAATTGGGTTCCACCACCG+GGG + chr5.4:30063411-30063430 None:intergenic 60.0%
GGGAATTGGGTTCCACCACC+GGG + chr5.4:30063412-30063431 None:intergenic 60.0%
GTGTTCAGATTCCCCGGTGG+TGG - chr5.4:30063397-30063416 MS.gene032884:intron 60.0%
! AGGGGTGTACCTGCCGTCAT+TGG - chr5.4:30063933-30063952 MS.gene032884:CDS 60.0%
! CCTGCCGTCATTGGTGTCCT+AGG - chr5.4:30063942-30063961 MS.gene032884:CDS 60.0%
! GTCAGGACAGCACTGGACAC+TGG - chr5.4:30063493-30063512 MS.gene032884:intron 60.0%
GGCACGCCGTTGATCATGGC+AGG + chr5.4:30063167-30063186 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 30062878 30065887 30062878 ID=MS.gene032884
chr5.4 mRNA 30062878 30065887 30062878 ID=MS.gene032884.t1;Parent=MS.gene032884
chr5.4 exon 30065243 30065887 30065243 ID=MS.gene032884.t1.exon1;Parent=MS.gene032884.t1
chr5.4 CDS 30065243 30065887 30065243 ID=cds.MS.gene032884.t1;Parent=MS.gene032884.t1
chr5.4 exon 30064721 30064931 30064721 ID=MS.gene032884.t1.exon2;Parent=MS.gene032884.t1
chr5.4 CDS 30064721 30064931 30064721 ID=cds.MS.gene032884.t1;Parent=MS.gene032884.t1
chr5.4 exon 30064339 30064633 30064339 ID=MS.gene032884.t1.exon3;Parent=MS.gene032884.t1
chr5.4 CDS 30064339 30064633 30064339 ID=cds.MS.gene032884.t1;Parent=MS.gene032884.t1
chr5.4 exon 30063797 30064165 30063797 ID=MS.gene032884.t1.exon4;Parent=MS.gene032884.t1
chr5.4 CDS 30063797 30064165 30063797 ID=cds.MS.gene032884.t1;Parent=MS.gene032884.t1
chr5.4 exon 30063323 30063400 30063323 ID=MS.gene032884.t1.exon5;Parent=MS.gene032884.t1
chr5.4 CDS 30063323 30063400 30063323 ID=cds.MS.gene032884.t1;Parent=MS.gene032884.t1
chr5.4 exon 30062878 30063109 30062878 ID=MS.gene032884.t1.exon6;Parent=MS.gene032884.t1
chr5.4 CDS 30062878 30063109 30062878 ID=cds.MS.gene032884.t1;Parent=MS.gene032884.t1
Gene Sequence

>MS.gene032884

ATGGCTAAACCAATTTCTTCTGGTAGTAGAACTAGAAGCTTTGTGCAGATCTTTATAGTAGTTGGTCTATGTTGTTTCTTCTACATATTAGGTGCGTGGCAGAGAACTGGTTTTGGAAAGGGAGATCTTTTACAATTGGAGGTTACTAAGAAAGGTGTTGGGTGTGATATTGTTCCAAATTTAAGCTTTGATTCTCACCATGGTGGAGAAGTTAGCAAAATTGATGAGGTTGATTCAAAACCTAAGGTATTTAAGCCTTGCAAGGCTCGATATATCGATTACACACCCTGCCATGATCAACGGCGTGCCATGACCTTTTCTAGACAAAATATGATTTATCGAGAGAGGCATTGCCCTCGTGAAGAAGAGAAGTTACATCGTTTGATACCAGCACCGAAAGGGTATGTTACTCCATTTCCATGGCCAAAGAGCCGTGATTATGTTCCATATGCGAACGCACCCTACAAGAGTCTTACAGTGGAAAAGGCTATTCAGAACTGGATCCAATATGAGGGAAATGTGTTCAGATTCCCCGGTGGTGGAACCCAATTCCCTCAAGGGGCTGACAAATATATTGATCAAATTGCTTCTGTGATACCGATAGAGAATGGAACAGTCAGGACAGCACTGGACACTGGTTGTGGGGTACGTTTGCCTGCGTGAATTCCTTAGGGCTTGTATCATATTCTTTTGTCTTTTTTCATGTTAATTTGATTAGCAGCATAGTCTAGTTTAGGGTTGTCAAATAGTAGCTATGCCGCTATAGCATAGCAGTATTTGAACAAATCGCTATTGTTATGTAATACGCTATTTAGTTCAAAGTGTTGTCAAATAGAGGCGCTATAGCACTGTAGTGGAATTTGAACAAACGAATATTTTCCATAATCCGCGACTAACATCACTGAGTTAGTTTGTTTTAGCTAGGTATCTGTAATGTGTAAGGTTGTTTTGCACAGGTTGCCAGTTGGGGTGCATATCTTTGGAGCAGAAATGTTATCACTATGTCATTTGCACCTAGGGACTCACATGAAGCACAAGTACAATTTGCACTTGAAAGGGGTGTACCTGCCGTCATTGGTGTCCTAGGAACCATAAAATTGCCTTATCCATCTGGAGCCTTTGATATGGCACATTGTTCTCGTTGCTTAATTCCTTGGGGATCAAATGGTGAGAAATTTACATTAAGAACGATAATTTTTCTTCTGAAGTACTTCGAGTAAATAACGAAAGTGCATCTTGGGTTGTTATTGGCAGATGGGATTTATATGATGGAAGTTGACCGAGTTCTAAGACCTGGCGGTTATTGGGTGCTTTCGGGACCTCCAATCCATTGGAAGGCTAATTACAAAGCCTGGCAAAGACCTAAGGAGGATCTTGAGGAAGAACAAAGAAAGATTGAAGAGGTTGCTAAGCTCCTTTGCTGGGAGAAGAAGACCGAGAAGAATGAAATTGCTGTCTGGCAGAAAACTGTTGATTCTGAAACATGTCGAAGTAGACAAGAAGATTCTGGTGCGAAGTTCTGTGAATCAACAGATGCTGATGATGTTTGGTATGTGCTTTCTTTCAGTGTTAAACATTTATGCATTTTAGCATCCATTGTTGGTATTCCATTAATTTAGTTCTACTAACAAACTAGATCATTCGAGCATGCATAAATATAAGAAAAAAGTTATGTACAAAAATCTTGTACTGAGTTGGAATGGATTTGTTTTGTCGTTGCAGGTATAAGAAAATGGAAGCCTGTGTTACTCCTAATCGTAAAGTCCACGGAGATCTTAAGCCTTTTCCACAGAGACTATATGCAGTTCCTCCTAAAGTTGCCAGTGGTTCTGTTCCCGGAGTTTCAGCTGAGACATACCAGGATGATAACGAAAAGTGGAAAAAACATGTAAATGCTTACAAGAAAATAAATAAGCTCCTGGACTCTGGAAGGTATCGCAATATCATGGATATGAATGCTGGTTTGGGTAGTTTTGCTGCTGCTATTCAATCTCCTAAGTTATGGGTCATGAATGTTGTGCCTACTATAGCTGAGAAACATACACTCGGTGTCATTTATCAGCGAGGACTCATAGGCATTTATCACGATTGGTATAGTTTTCTAACTTCTTCCTTCTATTTTATTCAGAGTGTCTTGATTTTATGTTTGAAAATGCTTAGTCTTGAACTATTATTGTTTTGAGAAATGACACTTTGACATCAAAATTTGATTTCAAATACAGTGTTCTTTGCATTTATGTGTATCTATGAAATGTGTGTAAATTGTAGAACATAAAAAGTAAGAGAAAGAGGTTAGAGATAAACATATACGGTGTCACATATGGTGTCTGTATTTTGATGTTCAAATAACACAACTCAATTCTTTTTCTCCTTGATGTATTGCATGAAAACGTAGACTCCGTCGTGTATTGTTAGCACAACTTTCTTTGATCGGGTGGCCCTTTTCTCGCATGTTTTTCCTTATTTATTTATGCCTTTATGATCCAGGTGTGAAGCTTTCTCCACATATCCAAGGACGTACGACCTCATTCATAGCAATGGGCTCTTTAGTCTGTACAAGGACAAGTGAGTATTCCTTTAATTATATGGTTATTCAATTCTTAATCAATAGTTTAGCCTTTGCATAGAATGCATTATAAGTTGTATAGGTGGTATGAAAATGATTTCAAGATAATGATTATCAGTGATAGAACCACTTAGTTTTAGTTCTCTCTTTCCCCTTTGCCAGTTGTGTTCATTCTTGTGTCTCAAAATCCTGTATCTAATCATCAAATGCAGATGTAACATAAATGACATTCTTCTGGAGATGGACCGGATTTTACGACCTGAAGGTGCTGTGATATTCCGTGATGAAGTTGATGTCTTAATCAAGGTAAAGAAAATTGTTGGAGGTATGAGATGGGATACTAAACTGGTTGACCATGAGGACGGTCCACTTGTTTCTGAAAAGATACTAATTGCTGTCAAGCAGTATTGGGTTGCTGATACAAATGCCACGTCAGCACAATAA
Protein sequence

>MS.gene032884.t1

MAKPISSGSRTRSFVQIFIVVGLCCFFYILGAWQRTGFGKGDLLQLEVTKKGVGCDIVPNLSFDSHHGGEVSKIDEVDSKPKVFKPCKARYIDYTPCHDQRRAMTFSRQNMIYRERHCPREEEKLHRLIPAPKGYVTPFPWPKSRDYVPYANAPYKSLTVEKAIQNWIQYEGNVFRFPGGGTQFPQGADKYIDQIASVIPIENGTVRTALDTGCGVASWGAYLWSRNVITMSFAPRDSHEAQVQFALERGVPAVIGVLGTIKLPYPSGAFDMAHCSRCLIPWGSNDGIYMMEVDRVLRPGGYWVLSGPPIHWKANYKAWQRPKEDLEEEQRKIEEVAKLLCWEKKTEKNEIAVWQKTVDSETCRSRQEDSGAKFCESTDADDVWYKKMEACVTPNRKVHGDLKPFPQRLYAVPPKVASGSVPGVSAETYQDDNEKWKKHVNAYKKINKLLDSGRYRNIMDMNAGLGSFAAAIQSPKLWVMNVVPTIAEKHTLGVIYQRGLIGIYHDWCEAFSTYPRTYDLIHSNGLFSLYKDKCNINDILLEMDRILRPEGAVIFRDEVDVLIKVKKIVGGMRWDTKLVDHEDGPLVSEKILIAVKQYWVADTNATSAQ