AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60370.t1 MTR_4g098890 98.835 601 7 0 1 601 1 601 0.0 1254
MS.gene60370.t1 MTR_8g055840 68.071 617 182 6 3 613 2 609 0.0 910
MS.gene60370.t1 MTR_5g040360 66.288 617 192 6 3 613 2 608 0.0 884
MS.gene60370.t1 MTR_3g040370 63.592 618 208 7 1 613 1 606 0.0 834
MS.gene60370.t1 MTR_8g100075 56.260 615 239 7 18 612 25 629 0.0 739
MS.gene60370.t1 MTR_7g076170 56.683 621 246 7 5 605 10 627 0.0 733
MS.gene60370.t1 MTR_0019s0120 56.209 612 249 6 17 612 27 635 0.0 730
MS.gene60370.t1 MTR_1g101370 57.167 600 249 4 13 604 19 618 0.0 728
MS.gene60370.t1 MTR_8g432680 54.173 611 252 6 18 605 27 632 0.0 696
MS.gene60370.t1 MTR_7g006060 52.841 528 238 4 82 598 39 566 0.0 595
MS.gene60370.t1 MTR_0019s0120 54.403 511 214 6 17 511 27 534 0.0 583
MS.gene60370.t1 MTR_8g071280 47.932 532 264 6 86 610 70 595 2.85e-178 518
MS.gene60370.t1 MTR_5g026930 44.243 608 309 11 10 610 13 597 3.09e-173 505
MS.gene60370.t1 MTR_7g076150 45.936 566 259 13 60 603 47 587 9.25e-167 488
MS.gene60370.t1 MTR_8g432620 44.102 551 276 9 67 604 70 601 1.20e-166 488
MS.gene60370.t1 MTR_5g080720 43.178 535 270 12 82 600 121 637 1.43e-151 451
MS.gene60370.t1 MTR_1g442840 42.173 543 280 11 84 606 90 618 4.70e-149 444
MS.gene60370.t1 MTR_1g442840 43.725 510 257 10 113 606 10 505 3.65e-147 436
MS.gene60370.t1 MTR_1g075900 41.261 555 292 11 72 606 77 617 4.67e-146 437
MS.gene60370.t1 MTR_3g068990 43.126 531 274 12 84 601 151 666 1.55e-144 434
MS.gene60370.t1 MTR_1g069785 40.255 549 294 11 84 612 60 594 8.23e-144 430
MS.gene60370.t1 MTR_1g029730 44.030 536 263 14 86 602 141 658 5.08e-143 430
MS.gene60370.t1 MTR_7g076150 47.974 469 199 12 155 603 1 444 6.16e-143 422
MS.gene60370.t1 MTR_3g096480 44.343 548 266 16 76 603 248 776 2.26e-141 430
MS.gene60370.t1 MTR_5g026930 44.204 509 255 10 10 511 13 499 8.16e-141 419
MS.gene60370.t1 MTR_8g093880 42.486 539 269 15 86 603 288 806 9.47e-135 414
MS.gene60370.t1 MTR_8g076650 44.078 515 259 13 95 594 387 887 5.59e-133 411
MS.gene60370.t1 MTR_1g051055 41.078 538 271 12 89 603 89 603 1.45e-132 401
MS.gene60370.t1 MTR_5g083150 39.811 530 282 14 94 610 100 605 4.91e-131 397
MS.gene60370.t1 MTR_5g080720 43.158 475 236 12 82 540 121 577 2.07e-130 395
MS.gene60370.t1 MTR_5g021100 41.714 525 271 14 89 594 322 830 1.90e-129 400
MS.gene60370.t1 MTR_3g088790 39.405 538 288 15 86 603 223 742 2.85e-123 382
MS.gene60370.t1 MTR_3g071530 39.400 500 274 12 91 579 149 630 5.23e-122 375
MS.gene60370.t1 MTR_1g022255 35.581 534 295 15 86 603 199 699 7.93e-96 309
MS.gene60370.t1 MTR_3g074780 32.200 559 322 17 82 603 150 688 6.22e-91 295
MS.gene60370.t1 MTR_4g083030 31.639 610 364 15 16 602 24 603 1.95e-90 292
MS.gene60370.t1 MTR_5g064080 31.794 563 321 18 76 603 136 670 3.27e-88 288
MS.gene60370.t1 MTR_1g022255 30.846 402 234 13 86 472 199 571 1.80e-46 173
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60370.t1 AT4G18030 74.794 607 149 3 1 605 1 605 0.0 970
MS.gene60370.t1 AT1G26850 68.720 617 184 5 1 610 1 615 0.0 912
MS.gene60370.t1 AT1G26850 68.720 617 184 5 1 610 1 615 0.0 912
MS.gene60370.t1 AT4G10440 56.981 616 240 6 11 609 15 622 0.0 753
MS.gene60370.t1 AT1G33170 54.874 636 253 6 3 607 6 638 0.0 729
MS.gene60370.t1 AT1G26850 67.723 505 154 5 1 498 1 503 0.0 725
MS.gene60370.t1 AT4G00750 55.025 607 261 6 18 612 25 631 0.0 716
MS.gene60370.t1 AT2G45750 54.248 612 263 8 18 613 19 629 0.0 689
MS.gene60370.t1 AT2G43200 51.731 520 240 5 86 598 91 606 0.0 563
MS.gene60370.t1 AT2G43200 51.731 520 240 5 86 598 91 606 0.0 563
MS.gene60370.t1 AT1G31850 44.928 621 309 13 2 610 4 603 0.0 538
MS.gene60370.t1 AT1G31850 44.928 621 309 13 2 610 4 603 0.0 538
MS.gene60370.t1 AT1G31850 44.928 621 309 13 2 610 4 603 0.0 538
MS.gene60370.t1 AT1G31850 44.928 621 309 13 2 610 4 603 0.0 538
MS.gene60370.t1 AT4G19120 44.545 550 290 6 73 613 57 600 7.21e-178 517
MS.gene60370.t1 AT4G19120 44.545 550 290 6 73 613 57 600 7.21e-178 517
MS.gene60370.t1 AT4G00740 44.505 546 275 9 69 603 65 593 5.14e-162 477
MS.gene60370.t1 AT4G00740 44.505 546 275 9 69 603 65 593 5.14e-162 477
MS.gene60370.t1 AT1G04430 38.622 624 342 12 9 606 8 616 2.46e-146 437
MS.gene60370.t1 AT1G04430 38.622 624 342 12 9 606 8 616 2.46e-146 437
MS.gene60370.t1 AT1G04430 38.622 624 342 12 9 606 8 616 2.46e-146 437
MS.gene60370.t1 AT5G06050 42.752 545 262 14 82 602 145 663 5.23e-146 438
MS.gene60370.t1 AT4G19120 45.759 448 229 5 73 511 57 499 7.65e-146 432
MS.gene60370.t1 AT2G39750 41.516 554 282 12 69 600 156 689 5.35e-144 434
MS.gene60370.t1 AT4G14360 42.690 513 263 10 84 579 74 572 7.05e-144 431
MS.gene60370.t1 AT4G14360 42.690 513 263 10 84 579 74 572 7.05e-144 431
MS.gene60370.t1 AT3G23300 40.433 554 297 11 72 606 65 604 1.63e-143 430
MS.gene60370.t1 AT3G23300 40.433 554 297 11 72 606 65 604 1.63e-143 430
MS.gene60370.t1 AT1G77260 43.097 536 259 15 86 600 143 653 2.67e-142 428
MS.gene60370.t1 AT1G29470 43.885 556 267 18 73 603 222 757 1.13e-139 425
MS.gene60370.t1 AT1G29470 43.885 556 267 18 73 603 222 757 1.13e-139 425
MS.gene60370.t1 AT5G14430 39.016 610 324 16 16 603 21 604 5.43e-136 410
MS.gene60370.t1 AT2G34300 42.937 538 267 15 86 603 240 757 1.72e-135 414
MS.gene60370.t1 AT2G34300 42.937 538 267 15 86 603 240 757 1.72e-135 414
MS.gene60370.t1 AT2G34300 42.937 538 267 15 86 603 240 757 1.72e-135 414
MS.gene60370.t1 AT5G04060 39.962 533 283 10 88 603 85 597 4.80e-135 407
MS.gene60370.t1 AT5G14430 38.689 610 326 16 16 603 21 604 3.50e-134 405
MS.gene60370.t1 AT3G56080 42.032 502 257 12 95 582 114 595 6.57e-134 405
MS.gene60370.t1 AT3G10200 40.706 538 287 11 81 603 68 588 1.99e-133 403
MS.gene60370.t1 AT5G64030 42.694 527 266 15 95 603 308 816 8.35e-133 409
MS.gene60370.t1 AT3G51070 42.453 530 265 14 94 603 380 889 5.60e-129 401
MS.gene60370.t1 AT2G40280 40.421 522 270 13 94 603 89 581 2.33e-127 387
MS.gene60370.t1 AT3G10200 40.234 512 273 11 81 576 68 562 1.48e-125 383
MS.gene60370.t1 AT3G56080 43.142 452 223 12 95 532 114 545 3.46e-119 365
MS.gene60370.t1 AT3G51070 43.982 457 224 12 94 532 380 822 9.75e-112 354
MS.gene60370.t1 AT1G19430 35.609 542 298 17 80 603 214 722 1.55e-94 306
MS.gene60370.t1 AT1G19430 35.609 542 298 17 80 603 214 722 1.55e-94 306
MS.gene60370.t1 AT1G13860 32.072 555 308 13 82 600 77 598 1.42e-89 290
MS.gene60370.t1 AT1G13860 32.072 555 308 13 82 600 77 598 1.42e-89 290
MS.gene60370.t1 AT1G13860 32.072 555 308 13 82 600 77 598 1.42e-89 290
MS.gene60370.t1 AT1G13860 32.072 555 308 13 82 600 77 598 1.42e-89 290
MS.gene60370.t1 AT1G78240 32.671 554 319 16 84 602 144 678 5.10e-89 290
MS.gene60370.t1 AT1G78240 32.671 554 319 16 84 602 144 678 5.10e-89 290
MS.gene60370.t1 AT2G03480 31.942 551 301 13 84 602 84 592 4.04e-86 280
MS.gene60370.t1 AT2G03480 32.545 550 310 14 84 602 84 603 1.50e-84 276
MS.gene60370.t1 AT1G13860 33.550 462 252 11 172 600 3 442 1.32e-79 259
MS.gene60370.t1 AT1G19430 31.873 411 234 15 80 473 214 595 2.02e-48 179
MS.gene60370.t1 AT2G03480 30.282 284 184 3 84 358 84 362 4.16e-41 155

Find 161 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATCTAAATCACATAAAT+TGG 0.157551 8.1:-23732999 None:intergenic
CCTGCTATTATTGGTGTTCT+TGG 0.181876 8.1:+23732474 MS.gene60370:CDS
TGCAAGGCTGTCTCCCTTTC+CGG 0.190229 8.1:-23731411 None:intergenic
TATCTGGCCCTCCAATTAAT+TGG 0.198353 8.1:+23732810 MS.gene60370:CDS
ATCAAGAGGACAACAAATTA+TGG 0.295703 8.1:+23733351 MS.gene60370:CDS
ACAAGGTTCAGAAAATTGTT+AGG 0.303090 8.1:+23734347 MS.gene60370:CDS
TAGAGATGGACCGCATATTA+AGG 0.304977 8.1:+23734281 MS.gene60370:CDS
CTAATACCATGGGCTGAAAA+TGG 0.306663 8.1:+23732555 MS.gene60370:CDS
TCTCTAAAAGGATGTCTTCT+AGG 0.313204 8.1:-23734264 None:intergenic
CAAACTGAACTTGTGCTTCA+TGG 0.317331 8.1:-23732434 None:intergenic
GGCTGGTTACCTCGGGGATA+GGG 0.326072 8.1:-23733223 None:intergenic
CCTTCGTTCGCTTCCATGTT+TGG 0.326669 8.1:-23732844 None:intergenic
ATATGCGGTCCATCTCTAAA+AGG 0.333621 8.1:-23734276 None:intergenic
CTAGAGAAAACATGATCTAT+AGG 0.334371 8.1:+23731621 MS.gene60370:CDS
TGGAGAAGGCTAATCAAAAC+TGG 0.334883 8.1:+23731780 MS.gene60370:CDS
CGAGGGGTACCTGCTATTAT+TGG 0.338287 8.1:+23732465 MS.gene60370:CDS
ATAACGTCTTTAGCTATTCT+AGG 0.339212 8.1:-23733305 None:intergenic
TACAATAACCAATATCTTCT+CGG 0.340229 8.1:-23734422 None:intergenic
GATTATGCAGGTTGCGAGTT+GGG 0.346051 8.1:+23732356 MS.gene60370:intron
ACAATTGCTGACAACACTTT+AGG 0.348076 8.1:+23733518 MS.gene60370:CDS
GGTTATTGTAAAAGAGTATT+GGG 0.355588 8.1:+23734435 MS.gene60370:CDS
ATTTGCACCCGAGAAGATAT+TGG 0.359714 8.1:+23734414 MS.gene60370:CDS
AATGAGATGGGAATCTAAAA+TGG 0.360431 8.1:+23734372 MS.gene60370:CDS
AATTTCTACATATTTGTGTT+GGG 0.361502 8.1:-23731498 None:intergenic
CGGATTATGACCGATCCTTC+TGG 0.383497 8.1:-23734304 None:intergenic
GGGAATGTGTTCAAATTTCC+AGG 0.385082 8.1:+23731815 MS.gene60370:CDS
TAGAGAAAACATGATCTATA+GGG 0.387569 8.1:+23731622 MS.gene60370:CDS
AATATCTTCTCGGGTGCAAA+TGG 0.387847 8.1:-23734412 None:intergenic
CAAGGTTCAGAAAATTGTTA+GGG 0.396641 8.1:+23734348 MS.gene60370:CDS
TCCGGCAACTTCACTTTGGC+TGG 0.396919 8.1:-23733240 None:intergenic
TGGCTGGTTACCTCGGGGAT+AGG 0.400033 8.1:-23733224 None:intergenic
ATAGCTAAAGACGTTATTCC+AGG 0.406709 8.1:+23733311 MS.gene60370:CDS
TCACTTTGGCTGGTTACCTC+GGG 0.406855 8.1:-23733230 None:intergenic
AAAGGAGTCGTGTACCCTTC+GGG 0.407789 8.1:-23731695 None:intergenic
CTGACAGTACCATCTTTGAT+TGG 0.409983 8.1:-23731899 None:intergenic
AAAAGGGCGATATCGCTATC+TGG 0.411310 8.1:+23732936 MS.gene60370:CDS
TAGATAATGCTGATGATGTT+TGG 0.414758 8.1:+23733014 MS.gene60370:CDS
GGGAGACAGCCTTGCATTGA+AGG 0.415396 8.1:+23731418 MS.gene60370:CDS
GTTCTCGTTGTCTAATACCA+TGG 0.415422 8.1:+23732544 MS.gene60370:CDS
CCGTGCTCTGTTGCCCGGTA+AGG 0.424595 8.1:-23731312 None:intergenic
TGGGGTTCAAAATTCAAATT+CGG 0.428738 8.1:-23731479 None:intergenic
ATCTAGCCGAAAGTCTTTGC+TGG 0.428753 8.1:+23732900 MS.gene60370:CDS
CTCTAGGGAATGTCATTGCT+CGG 0.429428 8.1:-23731604 None:intergenic
CAGCATTGGACACTGGTTGC+GGG 0.430729 8.1:+23731924 MS.gene60370:CDS
TCCACTGTCAGATGCTTGTA+AGG 0.431312 8.1:-23731761 None:intergenic
CAAAACTGGGTGAAATTTGA+AGG 0.440422 8.1:+23731794 MS.gene60370:CDS
TCATTGCTCGGTCTTGCTCT+TGG 0.442210 8.1:-23731592 None:intergenic
TGTGCTTCATGGTTATCCTT+CGG 0.442466 8.1:-23732423 None:intergenic
GTAAGGAACATTAGCATAGT+AGG 0.443219 8.1:-23731744 None:intergenic
TAAAGTGTTGTCAGCAATTG+TGG 0.446000 8.1:-23733516 None:intergenic
GTCGTGTACCCTTCGGGAGC+AGG 0.448795 8.1:-23731689 None:intergenic
AAAAGGAGTCGTGTACCCTT+CGG 0.449355 8.1:-23731696 None:intergenic
TGGTTATTGTAAAAGAGTAT+TGG 0.449942 8.1:+23734434 MS.gene60370:CDS
TTCACTTTGGCTGGTTACCT+CGG 0.450057 8.1:-23733231 None:intergenic
ACCGAAAGCTAAAATGTTCA+AGG 0.457641 8.1:+23731535 MS.gene60370:CDS
ACAATAACCAATATCTTCTC+GGG 0.458082 8.1:-23734421 None:intergenic
CTCCTCCGGCAACTTCACTT+TGG 0.458578 8.1:-23733244 None:intergenic
CAATTTCTACATATTTGTGT+TGG 0.459965 8.1:-23731499 None:intergenic
ATCTATGAAAGAGGTCTCAT+TGG 0.460262 8.1:+23733545 MS.gene60370:CDS
TGCAGAGGGCATGTATCTAA+TGG 0.460772 8.1:+23732748 MS.gene60370:intron
ATGGACATGAATGCAGGCCT+TGG 0.463464 8.1:+23733440 MS.gene60370:CDS
ACATGGCAAAGCAGTGGTTC+CGG 0.463502 8.1:+23731392 MS.gene60370:CDS
GGCCTCCGTGCTCTGTTGCC+CGG 0.469795 8.1:-23731317 None:intergenic
TTAGGTGTTATCTATGAAAG+AGG 0.473905 8.1:+23733536 MS.gene60370:CDS
CAAAGCAGTGGTTCCGGAAA+GGG 0.478553 8.1:+23731398 MS.gene60370:CDS
ACCAGCCAAAGTGAAGTTGC+CGG 0.483225 8.1:+23733239 MS.gene60370:CDS
GGGACTGCAAACAGCCTAGC+AGG 0.484901 8.1:-23733281 None:intergenic
GACATGAATGCAGGCCTTGG+AGG 0.485877 8.1:+23733443 MS.gene60370:CDS
TGATCGGCACCACTAGATAT+CGG 0.486791 8.1:+23733411 MS.gene60370:CDS
AGCACAAGTTCAGTTTGCAT+TGG 0.488386 8.1:+23732440 MS.gene60370:CDS
CTGGGAAAAGAAGTATGAAA+AGG 0.490567 8.1:+23732919 MS.gene60370:CDS
TCATTGGTATTTATCATGAC+TGG 0.490759 8.1:+23733561 MS.gene60370:CDS
AAACTTCTTCAATTCTCCTC+CGG 0.491193 8.1:-23733258 None:intergenic
TATACGCATCGGCTCCTTGA+GGG 0.491733 8.1:-23731853 None:intergenic
GGGAACATGGTTCCTCCACC+TGG 0.492570 8.1:-23731833 None:intergenic
TATGATCTTATTCATGCCAA+TGG 0.492874 8.1:+23733769 MS.gene60370:CDS
ATTGTAAAAGAGTATTGGGT+TGG 0.493911 8.1:+23734439 MS.gene60370:CDS
TTATCTAAATCACATAAATT+GGG 0.494725 8.1:-23732998 None:intergenic
TGAATAAGATCATATGTCCT+TGG 0.500320 8.1:-23733760 None:intergenic
ACAGCATTGGACACTGGTTG+CGG 0.504094 8.1:+23731923 MS.gene60370:CDS
AAAAGAATTAATAGATTGAT+CGG 0.507870 8.1:+23733395 MS.gene60370:CDS
TGCCTCATTCCTGCTCCCGA+AGG 0.510952 8.1:+23731680 MS.gene60370:CDS
AGGAAGAAACTTACTTGTCC+TGG 0.512195 8.1:-23733807 None:intergenic
AGCTATGTCATTTGCTCCGA+AGG 0.512623 8.1:+23732407 MS.gene60370:CDS
AATTGTTAGGGGAATGAGAT+GGG 0.515509 8.1:+23734360 MS.gene60370:CDS
AACAGTTGATTCTTATCAAG+AGG 0.515523 8.1:+23733337 MS.gene60370:CDS
GCTCGGTCTTGCTCTTGGCA+AGG 0.517894 8.1:-23731587 None:intergenic
GTGTTCAAATTTCCAGGTGG+AGG 0.519025 8.1:+23731821 MS.gene60370:CDS
TAAGAATCAACTGTTACGCC+TGG 0.523031 8.1:-23733329 None:intergenic
CAAGCATCTGACAGTGGAGA+AGG 0.525351 8.1:+23731766 MS.gene60370:CDS
TCAAAGGCTCGTGATGGGAA+TGG 0.531393 8.1:-23732510 None:intergenic
GTACAAACTAAATAAACCAT+TGG 0.532852 8.1:-23733785 None:intergenic
TCTTATTTACCTTCAATGCA+AGG 0.533350 8.1:-23731427 None:intergenic
ATTATGCAGGTTGCGAGTTG+GGG 0.534569 8.1:+23732357 MS.gene60370:intron
ATTGAAGGTAAATAAGATGC+AGG 0.535476 8.1:+23731433 MS.gene60370:CDS
CACTAGATATCGGAATGTAA+TGG 0.536983 8.1:+23733421 MS.gene60370:CDS
GCTGGTTACCTCGGGGATAG+GGG 0.539426 8.1:-23733222 None:intergenic
TTCTCGTTGTCTAATACCAT+GGG 0.539833 8.1:+23732545 MS.gene60370:CDS
GACATATCAAAGGCTCGTGA+TGG 0.541258 8.1:-23732516 None:intergenic
ATCTAAAATGGTGGATCATG+AGG 0.541586 8.1:+23734384 MS.gene60370:CDS
ATTTCTACATATTTGTGTTG+GGG 0.545040 8.1:-23731497 None:intergenic
TGGTTTATTTAGTTTGTACC+AGG 0.546436 8.1:+23733789 MS.gene60370:CDS
ATATCTTCTCGGGTGCAAAT+GGG 0.548383 8.1:-23734411 None:intergenic
GCCTCATTCCTGCTCCCGAA+GGG 0.551559 8.1:+23731681 MS.gene60370:CDS
ACCCTTCGGGAGCAGGAATG+AGG 0.552400 8.1:-23731682 None:intergenic
CATATTAAGGCCAGAAGGAT+CGG 0.556558 8.1:+23734294 MS.gene60370:CDS
TTCAGTTTGCATTGGAACGA+GGG 0.556579 8.1:+23732448 MS.gene60370:CDS
AAAACATGATCTATAGGGAA+AGG 0.562202 8.1:+23731627 MS.gene60370:CDS
AATTGAAGAAGTTTCCTGCT+AGG 0.562951 8.1:+23733267 MS.gene60370:CDS
TGATTATGCAGGTTGCGAGT+TGG 0.564155 8.1:+23732355 MS.gene60370:intron
TCGGTTATTCCAATCAAAGA+TGG 0.565140 8.1:+23731890 MS.gene60370:CDS
GCAAAGCAGTGGTTCCGGAA+AGG 0.568170 8.1:+23731397 MS.gene60370:CDS
CCTTACCGGGCAACAGAGCA+CGG 0.569031 8.1:+23731312 MS.gene60370:CDS
GCGAACGAAGGAGGATCTGA+AGG 0.569849 8.1:+23732856 MS.gene60370:CDS
CGTCTTTAGCTATTCTAGGA+GGG 0.573557 8.1:-23733301 None:intergenic
CCAAGAACACCAATAATAGC+AGG 0.574717 8.1:-23732474 None:intergenic
AATGTAATGGACATGAATGC+AGG 0.577859 8.1:+23733434 MS.gene60370:CDS
ATCCTTCTGGCCTTAATATG+CGG 0.578717 8.1:-23734291 None:intergenic
TGGGAAAAGAAGTATGAAAA+GGG 0.579153 8.1:+23732920 MS.gene60370:CDS
ATCGGCTCCTTGAGGGAACA+TGG 0.579987 8.1:-23731846 None:intergenic
AAGGCATTGTCCACCTCAAG+AGG 0.580231 8.1:+23731646 MS.gene60370:CDS
ACGTCTTTAGCTATTCTAGG+AGG 0.584489 8.1:-23733302 None:intergenic
ACATATCAAAGGCTCGTGAT+GGG 0.586233 8.1:-23732515 None:intergenic
TCTAGCCGAAAGTCTTTGCT+GGG 0.586847 8.1:+23732901 MS.gene60370:CDS
CATTACATTCCGATATCTAG+TGG 0.587708 8.1:-23733420 None:intergenic
GTTCAGTTTGCATTGGAACG+AGG 0.591548 8.1:+23732447 MS.gene60370:CDS
TGGTACTGTCAGAACAGCAT+TGG 0.594187 8.1:+23731910 MS.gene60370:CDS
CTCGTGATGGGAATGGAAGA+CGG 0.594874 8.1:-23732503 None:intergenic
CGAAGTTGATGTGATTAACA+AGG 0.596587 8.1:+23734330 MS.gene60370:CDS
TTGGGAACATGGCAAAGCAG+TGG 0.599535 8.1:+23731386 MS.gene60370:CDS
GTGCAGCTGCAAATCCTCCA+AGG 0.600414 8.1:-23733457 None:intergenic
GGAAAACGTACTACCAAACA+TGG 0.600730 8.1:+23732831 MS.gene60370:CDS
AAAACTGGGTGAAATTTGAA+GGG 0.602703 8.1:+23731795 MS.gene60370:CDS
AGCATTGGACACTGGTTGCG+GGG 0.605572 8.1:+23731925 MS.gene60370:CDS
AAAGTGTTGTCAGCAATTGT+GGG 0.608729 8.1:-23733515 None:intergenic
AAATTGTTAGGGGAATGAGA+TGG 0.612828 8.1:+23734359 MS.gene60370:CDS
GAGATGGGAATCTAAAATGG+TGG 0.616902 8.1:+23734375 MS.gene60370:CDS
GTCAGAACAGCATTGGACAC+TGG 0.619837 8.1:+23731917 MS.gene60370:CDS
AAGCTCATCAATATACGCAT+CGG 0.620380 8.1:-23731864 None:intergenic
AACATGGAAGCGAACGAAGG+AGG 0.623111 8.1:+23732847 MS.gene60370:CDS
AAAATGGTGGATCATGAGGA+TGG 0.624067 8.1:+23734388 MS.gene60370:CDS
GGAGGAACCATGTTCCCTCA+AGG 0.625347 8.1:+23731839 MS.gene60370:CDS
CACTTTGGCTGGTTACCTCG+GGG 0.630898 8.1:-23733229 None:intergenic
GTATATTGATGAGCTTGCAT+CGG 0.639331 8.1:+23731871 MS.gene60370:CDS
ATATACGCATCGGCTCCTTG+AGG 0.645967 8.1:-23731854 None:intergenic
GACCGCATATTAAGGCCAGA+AGG 0.646017 8.1:+23734289 MS.gene60370:CDS
GGAGAAGGCTAATCAAAACT+GGG 0.651414 8.1:+23731781 MS.gene60370:CDS
CCAAACATGGAAGCGAACGA+AGG 0.652207 8.1:+23732844 MS.gene60370:CDS
TAGCATAGTAGGCATAATCA+CGG 0.653977 8.1:-23731733 None:intergenic
GATAAAATCCAAAATCCACC+AGG 0.658663 8.1:-23732791 None:intergenic
TCAGTTTGCATTGGAACGAG+GGG 0.665623 8.1:+23732449 MS.gene60370:CDS
AGCACAGCGAAAATTGACAA+AGG 0.667719 8.1:-23731338 None:intergenic
AAGGTTCAGAAAATTGTTAG+GGG 0.677847 8.1:+23734349 MS.gene60370:CDS
TCCTTACAAGCATCTGACAG+TGG 0.686028 8.1:+23731760 MS.gene60370:CDS
AGCCAAAGTGAAGTTGCCGG+AGG 0.689567 8.1:+23733242 MS.gene60370:CDS
AGAACACTGAGACATATCAA+AGG 0.695313 8.1:-23732526 None:intergenic
AGGGCGATATCGCTATCTGG+AGG 0.701556 8.1:+23732939 MS.gene60370:CDS
TAATCACATCAACTTCGTCG+CGG 0.731217 8.1:-23734324 None:intergenic
AATGTGTTCAAATTTCCAGG+TGG 0.732268 8.1:+23731818 MS.gene60370:CDS
CTGCAAAACCCCTATCCCCG+AGG 0.738570 8.1:+23733214 MS.gene60370:CDS
TACCGGGCAACAGAGCACGG+AGG 0.774215 8.1:+23731315 MS.gene60370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATTAAACTGATATTTA+TGG + chr8.1:23733665-23733684 MS.gene60370:intron 10.0%
!! AAAAGAATTAATAGATTGAT+CGG + chr8.1:23733395-23733414 MS.gene60370:CDS 15.0%
!! AAATAACATACTATATCAAA+AGG - chr8.1:23731972-23731991 None:intergenic 15.0%
!! AATTATGTGTAATGTATAAT+GGG + chr8.1:23732159-23732178 MS.gene60370:intron 15.0%
!! ATGATTATATTTAGAAGTTA+AGG - chr8.1:23732660-23732679 None:intergenic 15.0%
!! ATTATCTAAATCACATAAAT+TGG - chr8.1:23733002-23733021 None:intergenic 15.0%
!! TAATTATGTGTAATGTATAA+TGG + chr8.1:23732158-23732177 MS.gene60370:intron 15.0%
!! TTATCTAAATCACATAAATT+GGG - chr8.1:23733001-23733020 None:intergenic 15.0%
!!! AATTAATCATTTTAGAGTAT+AGG - chr8.1:23733652-23733671 None:intergenic 15.0%
!!! TATTTATGGATATTTTGTTA+TGG + chr8.1:23733679-23733698 MS.gene60370:intron 15.0%
!! AAATTTGACATCAAATTTGA+TGG + chr8.1:23732020-23732039 MS.gene60370:intron 20.0%
!! AATTTCTACATATTTGTGTT+GGG - chr8.1:23731501-23731520 None:intergenic 20.0%
!! ATTATGTGTAATGTATAATG+GGG + chr8.1:23732160-23732179 MS.gene60370:intron 20.0%
!! GATGATTAATGAATATAACA+AGG + chr8.1:23732078-23732097 MS.gene60370:intron 20.0%
!! TTAAACACATTTGAAAATTG+TGG - chr8.1:23732141-23732160 None:intergenic 20.0%
!! TTGATACCATCTTAAAATTT+AGG - chr8.1:23734071-23734090 None:intergenic 20.0%
!!! TGTTGTTTGTTTTATCTTTT+GGG + chr8.1:23731368-23731387 MS.gene60370:CDS 20.0%
!!! TTTTTTTTTCCTAGTAGATT+AGG - chr8.1:23734169-23734188 None:intergenic 20.0%
! AAGTCTATTCAAGATTTCAT+TGG + chr8.1:23734090-23734109 MS.gene60370:intron 25.0%
! ACACTGAAAATTGAGAAATT+TGG - chr8.1:23732202-23732221 None:intergenic 25.0%
! ACTTGAATCACAAAAATCTT+GGG + chr8.1:23733478-23733497 MS.gene60370:CDS 25.0%
! ATTTCTACATATTTGTGTTG+GGG - chr8.1:23731500-23731519 None:intergenic 25.0%
! CAATTTCTACATATTTGTGT+TGG - chr8.1:23731502-23731521 None:intergenic 25.0%
! CCTGAAAATATGTTTATAAG+TGG + chr8.1:23733992-23734011 MS.gene60370:intron 25.0%
! GTAAGCATTAAAACAAAGAA+AGG - chr8.1:23732255-23732274 None:intergenic 25.0%
! GTACAAACTAAATAAACCAT+TGG - chr8.1:23733788-23733807 None:intergenic 25.0%
! TAATGGTAGGTACAAAAAAA+TGG + chr8.1:23733187-23733206 MS.gene60370:intron 25.0%
! TACAATAACCAATATCTTCT+CGG - chr8.1:23734425-23734444 None:intergenic 25.0%
! TAGAGAAAACATGATCTATA+GGG + chr8.1:23731622-23731641 MS.gene60370:CDS 25.0%
! TGTATTTAGTATACTGAAGT+CGG + chr8.1:23732219-23732238 MS.gene60370:intron 25.0%
! TTCATATAATTCCACGATTT+CGG + chr8.1:23733118-23733137 MS.gene60370:intron 25.0%
!! CCACTTATAAACATATTTTC+AGG - chr8.1:23733995-23734014 None:intergenic 25.0%
!! GATCTAGAATACAGTATTAT+CGG + chr8.1:23732608-23732627 MS.gene60370:intron 25.0%
!! TAGTACGTTTTCCAATTAAT+TGG - chr8.1:23732824-23732843 None:intergenic 25.0%
!! TATAGATCATGTTTTCTCTA+GGG - chr8.1:23731622-23731641 None:intergenic 25.0%
!! TGGTTATTGTAAAAGAGTAT+TGG + chr8.1:23734434-23734453 MS.gene60370:CDS 25.0%
!!! GGTTATTGTAAAAGAGTATT+GGG + chr8.1:23734435-23734454 MS.gene60370:CDS 25.0%
!!! GTGTTGTTTGTTTTATCTTT+TGG + chr8.1:23731367-23731386 MS.gene60370:CDS 25.0%
!!! TTCTTTGTTTTAATGCTTAC+TGG + chr8.1:23732255-23732274 MS.gene60370:intron 25.0%
!!! TTTACTGATTTTTCCAATTC+TGG + chr8.1:23733922-23733941 MS.gene60370:intron 25.0%
AAAACATGATCTATAGGGAA+AGG + chr8.1:23731627-23731646 MS.gene60370:CDS 30.0%
AAAACTGGGTGAAATTTGAA+GGG + chr8.1:23731795-23731814 MS.gene60370:CDS 30.0%
AAGGTTCAGAAAATTGTTAG+GGG + chr8.1:23734349-23734368 MS.gene60370:CDS 30.0%
AATGAGATGGGAATCTAAAA+TGG + chr8.1:23734372-23734391 MS.gene60370:CDS 30.0%
ACAAGGTTCAGAAAATTGTT+AGG + chr8.1:23734347-23734366 MS.gene60370:CDS 30.0%
ACAATAACCAATATCTTCTC+GGG - chr8.1:23734424-23734443 None:intergenic 30.0%
ACAATCAAGAAGCAATTGAA+AGG - chr8.1:23732315-23732334 None:intergenic 30.0%
AGGTCTAATCTTATCAAATG+TGG - chr8.1:23733975-23733994 None:intergenic 30.0%
AGTCTCAATTATACCAGAAT+TGG - chr8.1:23733938-23733957 None:intergenic 30.0%
ATAACGTCTTTAGCTATTCT+AGG - chr8.1:23733308-23733327 None:intergenic 30.0%
ATCAAGAGGACAACAAATTA+TGG + chr8.1:23733351-23733370 MS.gene60370:CDS 30.0%
ATTATCACTATGATCACTTG+TGG - chr8.1:23733840-23733859 None:intergenic 30.0%
ATTGAAGGTAAATAAGATGC+AGG + chr8.1:23731433-23731452 MS.gene60370:CDS 30.0%
CAAGGTTCAGAAAATTGTTA+GGG + chr8.1:23734348-23734367 MS.gene60370:CDS 30.0%
CAAGTGATCATAGTGATAAT+TGG + chr8.1:23733840-23733859 MS.gene60370:intron 30.0%
CACTTGAATCACAAAAATCT+TGG + chr8.1:23733477-23733496 MS.gene60370:CDS 30.0%
CTAGAGAAAACATGATCTAT+AGG + chr8.1:23731621-23731640 MS.gene60370:CDS 30.0%
GAAATGTGTAGAAATGATCA+GGG - chr8.1:23733044-23733063 None:intergenic 30.0%
GGTCTAATCTTATCAAATGT+GGG - chr8.1:23733974-23733993 None:intergenic 30.0%
GTCTAATCTTATCAAATGTG+GGG - chr8.1:23733973-23733992 None:intergenic 30.0%
TATGATCTTATTCATGCCAA+TGG + chr8.1:23733769-23733788 MS.gene60370:CDS 30.0%
TCATTGGTATTTATCATGAC+TGG + chr8.1:23733561-23733580 MS.gene60370:CDS 30.0%
TCTTACAAAATCAGCTTGTA+AGG - chr8.1:23734031-23734050 None:intergenic 30.0%
TGAAATGTGTAGAAATGATC+AGG - chr8.1:23733045-23733064 None:intergenic 30.0%
TGAATAAGATCATATGTCCT+TGG - chr8.1:23733763-23733782 None:intergenic 30.0%
TGGGGTTCAAAATTCAAATT+CGG - chr8.1:23731482-23731501 None:intergenic 30.0%
! AACAGTTGATTCTTATCAAG+AGG + chr8.1:23733337-23733356 MS.gene60370:CDS 30.0%
! ACGTTTTCCAATTAATTGGA+GGG - chr8.1:23732820-23732839 None:intergenic 30.0%
! CTATAGATCATGTTTTCTCT+AGG - chr8.1:23731623-23731642 None:intergenic 30.0%
! GTACCTACCATTACAAATTT+TGG - chr8.1:23733180-23733199 None:intergenic 30.0%
! TACGTTTTCCAATTAATTGG+AGG - chr8.1:23732821-23732840 None:intergenic 30.0%
! TAGATAATGCTGATGATGTT+TGG + chr8.1:23733014-23733033 MS.gene60370:CDS 30.0%
! TCTTATTTACCTTCAATGCA+AGG - chr8.1:23731430-23731449 None:intergenic 30.0%
! TGGGAAAAGAAGTATGAAAA+GGG + chr8.1:23732920-23732939 MS.gene60370:CDS 30.0%
! TTAGGTGTTATCTATGAAAG+AGG + chr8.1:23733536-23733555 MS.gene60370:CDS 30.0%
!! ATTGTAAAAGAGTATTGGGT+TGG + chr8.1:23734439-23734458 MS.gene60370:CDS 30.0%
!! GTATTGTTGATTTGATTGAC+AGG + chr8.1:23734236-23734255 MS.gene60370:intron 30.0%
!! TATAATGGGGTTTTGTCTAT+TGG + chr8.1:23732173-23732192 MS.gene60370:intron 30.0%
!! TGGTTTATTTAGTTTGTACC+AGG + chr8.1:23733789-23733808 MS.gene60370:CDS 30.0%
!! TGTTTTATCTTTTGGGAACA+TGG + chr8.1:23731375-23731394 MS.gene60370:CDS 30.0%
AAACTTCTTCAATTCTCCTC+CGG - chr8.1:23733261-23733280 None:intergenic 35.0%
AAATTGTTAGGGGAATGAGA+TGG + chr8.1:23734359-23734378 MS.gene60370:CDS 35.0%
AAGCTCATCAATATACGCAT+CGG - chr8.1:23731867-23731886 None:intergenic 35.0%
AATGTGTTCAAATTTCCAGG+TGG + chr8.1:23731818-23731837 MS.gene60370:CDS 35.0%
AATTGAAGAAGTTTCCTGCT+AGG + chr8.1:23733267-23733286 MS.gene60370:CDS 35.0%
AATTGTTAGGGGAATGAGAT+GGG + chr8.1:23734360-23734379 MS.gene60370:CDS 35.0%
ACAATTGCTGACAACACTTT+AGG + chr8.1:23733518-23733537 MS.gene60370:CDS 35.0%
ACCCAATAATTGAATGCAGA+GGG + chr8.1:23732734-23732753 MS.gene60370:intron 35.0%
ACCGAAAGCTAAAATGTTCA+AGG + chr8.1:23731535-23731554 MS.gene60370:CDS 35.0%
AGAACACTGAGACATATCAA+AGG - chr8.1:23732529-23732548 None:intergenic 35.0%
ATAAGCGTGAATGATTATGC+AGG + chr8.1:23732344-23732363 MS.gene60370:intron 35.0%
ATACTGAAAGGAAGTAAACC+GGG - chr8.1:23732289-23732308 None:intergenic 35.0%
ATAGCTAAAGACGTTATTCC+AGG + chr8.1:23733311-23733330 MS.gene60370:CDS 35.0%
ATCTATGAAAGAGGTCTCAT+TGG + chr8.1:23733545-23733564 MS.gene60370:CDS 35.0%
ATGGTTACTATTCAGATGTG+TGG + chr8.1:23732039-23732058 MS.gene60370:intron 35.0%
CAAAACTGGGTGAAATTTGA+AGG + chr8.1:23731794-23731813 MS.gene60370:CDS 35.0%
CACTAGATATCGGAATGTAA+TGG + chr8.1:23733421-23733440 MS.gene60370:CDS 35.0%
CATACTGAAAGGAAGTAAAC+CGG - chr8.1:23732290-23732309 None:intergenic 35.0%
CATTACATTCCGATATCTAG+TGG - chr8.1:23733423-23733442 None:intergenic 35.0%
CCATGCTCCAAAATTTGTAA+TGG + chr8.1:23733170-23733189 MS.gene60370:intron 35.0%
GATAAAATCCAAAATCCACC+AGG - chr8.1:23732794-23732813 None:intergenic 35.0%
GCTCCAAAATTTGTAATGGT+AGG + chr8.1:23733174-23733193 MS.gene60370:intron 35.0%
GTAAGGAACATTAGCATAGT+AGG - chr8.1:23731747-23731766 None:intergenic 35.0%
GTATATTGATGAGCTTGCAT+CGG + chr8.1:23731871-23731890 MS.gene60370:CDS 35.0%
TAGCATAGTAGGCATAATCA+CGG - chr8.1:23731736-23731755 None:intergenic 35.0%
TCGGTTATTCCAATCAAAGA+TGG + chr8.1:23731890-23731909 MS.gene60370:CDS 35.0%
TGGTAGGTACAAAAAAATGG+AGG + chr8.1:23733190-23733209 MS.gene60370:intron 35.0%
TTCTCGTTGTCTAATACCAT+GGG + chr8.1:23732545-23732564 MS.gene60370:CDS 35.0%
! AAGGCTTTTCTACATATCCA+AGG + chr8.1:23733743-23733762 MS.gene60370:CDS 35.0%
! ATTGAAAGGTGCATACTGAA+AGG - chr8.1:23732301-23732320 None:intergenic 35.0%
! CTGGGAAAAGAAGTATGAAA+AGG + chr8.1:23732919-23732938 MS.gene60370:CDS 35.0%
! GACATTCGTTTTCTTTCTTC+AGG + chr8.1:23733716-23733735 MS.gene60370:intron 35.0%
! TCTCTAAAAGGATGTCTTCT+AGG - chr8.1:23734267-23734286 None:intergenic 35.0%
! TTTTCCTAGTAGATTAGGAC+TGG - chr8.1:23734164-23734183 None:intergenic 35.0%
! TTTTCTTTCTTCAGGTGTGA+AGG + chr8.1:23733724-23733743 MS.gene60370:intron 35.0%
!! AAAGTGTTGTCAGCAATTGT+GGG - chr8.1:23733518-23733537 None:intergenic 35.0%
!! AATGTAATGGACATGAATGC+AGG + chr8.1:23733434-23733453 MS.gene60370:CDS 35.0%
!! AGAAGACATCCTTTTAGAGA+TGG + chr8.1:23734267-23734286 MS.gene60370:CDS 35.0%
!! ATCTAAAATGGTGGATCATG+AGG + chr8.1:23734384-23734403 MS.gene60370:CDS 35.0%
!! CGAAGTTGATGTGATTAACA+AGG + chr8.1:23734330-23734349 MS.gene60370:CDS 35.0%
!! GCTCGACCTAAATTTTAAGA+TGG + chr8.1:23734062-23734081 MS.gene60370:intron 35.0%
!! GGTGGATTTTGGATTTTATC+TGG + chr8.1:23732794-23732813 MS.gene60370:CDS 35.0%
!! TAAAGTGTTGTCAGCAATTG+TGG - chr8.1:23733519-23733538 None:intergenic 35.0%
!! TGGAAGTTGATCGAGTTTTA+AGG + chr8.1:23732768-23732787 MS.gene60370:CDS 35.0%
!!! CCATTACAAATTTTGGAGCA+TGG - chr8.1:23733173-23733192 None:intergenic 35.0%
!!! GAACATTTTAGCTTTCGGTT+CGG - chr8.1:23731534-23731553 None:intergenic 35.0%
!!! GCCTTGAACATTTTAGCTTT+CGG - chr8.1:23731539-23731558 None:intergenic 35.0%
AATATCTTCTCGGGTGCAAA+TGG - chr8.1:23734415-23734434 None:intergenic 40.0%
ACATATCAAAGGCTCGTGAT+GGG - chr8.1:23732518-23732537 None:intergenic 40.0%
ACGTCTTTAGCTATTCTAGG+AGG - chr8.1:23733305-23733324 None:intergenic 40.0%
AGCACAAGTTCAGTTTGCAT+TGG + chr8.1:23732440-23732459 MS.gene60370:CDS 40.0%
AGCACAGCGAAAATTGACAA+AGG - chr8.1:23731341-23731360 None:intergenic 40.0%
AGGAAGAAACTTACTTGTCC+TGG - chr8.1:23733810-23733829 None:intergenic 40.0%
ATATCTTCTCGGGTGCAAAT+GGG - chr8.1:23734414-23734433 None:intergenic 40.0%
ATATGCGGTCCATCTCTAAA+AGG - chr8.1:23734279-23734298 None:intergenic 40.0%
ATTTGCACCCGAGAAGATAT+TGG + chr8.1:23734414-23734433 MS.gene60370:CDS 40.0%
CAAAATCAGCTTGTAAGGTG+AGG - chr8.1:23734026-23734045 None:intergenic 40.0%
CAAACTGAACTTGTGCTTCA+TGG - chr8.1:23732437-23732456 None:intergenic 40.0%
CAACTCATTAACCATCCACT+GGG + chr8.1:23732106-23732125 MS.gene60370:intron 40.0%
CACCCAATAATTGAATGCAG+AGG + chr8.1:23732733-23732752 MS.gene60370:intron 40.0%
CATATTAAGGCCAGAAGGAT+CGG + chr8.1:23734294-23734313 MS.gene60370:CDS 40.0%
CCAAGAACACCAATAATAGC+AGG - chr8.1:23732477-23732496 None:intergenic 40.0%
CGCATTATAATCCGAAATCG+TGG - chr8.1:23733132-23733151 None:intergenic 40.0%
CTGACAGTACCATCTTTGAT+TGG - chr8.1:23731902-23731921 None:intergenic 40.0%
GCCCTCTGCATTCAATTATT+GGG - chr8.1:23732738-23732757 None:intergenic 40.0%
GGAAAACGTACTACCAAACA+TGG + chr8.1:23732831-23732850 MS.gene60370:CDS 40.0%
GGAGAAGGCTAATCAAAACT+GGG + chr8.1:23731781-23731800 MS.gene60370:CDS 40.0%
GGGAATGTGTTCAAATTTCC+AGG + chr8.1:23731815-23731834 MS.gene60370:CDS 40.0%
GTTCTCGTTGTCTAATACCA+TGG + chr8.1:23732544-23732563 MS.gene60370:CDS 40.0%
TAAGAATCAACTGTTACGCC+TGG - chr8.1:23733332-23733351 None:intergenic 40.0%
TAATCACATCAACTTCGTCG+CGG - chr8.1:23734327-23734346 None:intergenic 40.0%
TAGAGATGGACCGCATATTA+AGG + chr8.1:23734281-23734300 MS.gene60370:CDS 40.0%
TATCTGGCCCTCCAATTAAT+TGG + chr8.1:23732810-23732829 MS.gene60370:CDS 40.0%
TCACTATGATCACTTGTGGA+TGG - chr8.1:23733836-23733855 None:intergenic 40.0%
TGATCACTTGTGGATGGAAA+AGG - chr8.1:23733830-23733849 None:intergenic 40.0%
TGCCCTCTGCATTCAATTAT+TGG - chr8.1:23732739-23732758 None:intergenic 40.0%
TGGAGAAGGCTAATCAAAAC+TGG + chr8.1:23731780-23731799 MS.gene60370:CDS 40.0%
TGTGCTTCATGGTTATCCTT+CGG - chr8.1:23732426-23732445 None:intergenic 40.0%
TTCAGTTTGCATTGGAACGA+GGG + chr8.1:23732448-23732467 MS.gene60370:CDS 40.0%
! CAACGCAATTTTTCCTCTTG+AGG - chr8.1:23731662-23731681 None:intergenic 40.0%
! CCTGCTATTATTGGTGTTCT+TGG + chr8.1:23732474-23732493 MS.gene60370:CDS 40.0%
! CGTCTTTAGCTATTCTAGGA+GGG - chr8.1:23733304-23733323 None:intergenic 40.0%
! CTAATACCATGGGCTGAAAA+TGG + chr8.1:23732555-23732574 MS.gene60370:CDS 40.0%
! CTTTTCCCAGCAAAGACTTT+CGG - chr8.1:23732909-23732928 None:intergenic 40.0%
! GAGATGGGAATCTAAAATGG+TGG + chr8.1:23734375-23734394 MS.gene60370:CDS 40.0%
! TAGGCATAATCACGGCTTTT+AGG - chr8.1:23731728-23731747 None:intergenic 40.0%
!! AAAATGGTGGATCATGAGGA+TGG + chr8.1:23734388-23734407 MS.gene60370:CDS 40.0%
!! ATCCTTCTGGCCTTAATATG+CGG - chr8.1:23734294-23734313 None:intergenic 40.0%
!! GGGTTCTAAAAATGCCTTAC+CGG + chr8.1:23731298-23731317 MS.gene60370:CDS 40.0%
!! GGTTCTAAAAATGCCTTACC+GGG + chr8.1:23731299-23731318 MS.gene60370:CDS 40.0%
!! TATAGGAAGCTGACTTGCAA+GGG - chr8.1:23733635-23733654 None:intergenic 40.0%
!!! TTTTAAGGCCTGGTGGATTT+TGG + chr8.1:23732783-23732802 MS.gene60370:CDS 40.0%
AAAAGGAGTCGTGTACCCTT+CGG - chr8.1:23731699-23731718 None:intergenic 45.0%
AAAAGGGCGATATCGCTATC+TGG + chr8.1:23732936-23732955 MS.gene60370:CDS 45.0%
ACCATTAGGTCTCCTGCTAT+CGG + chr8.1:23734120-23734139 MS.gene60370:intron 45.0%
ATAGCAGGAGACCTAATGGT+GGG - chr8.1:23734120-23734139 None:intergenic 45.0%
ATCTAGCCGAAAGTCTTTGC+TGG + chr8.1:23732900-23732919 MS.gene60370:CDS 45.0%
ATTATGCAGGTTGCGAGTTG+GGG + chr8.1:23732357-23732376 MS.gene60370:intron 45.0%
GACATATCAAAGGCTCGTGA+TGG - chr8.1:23732519-23732538 None:intergenic 45.0%
GATTATGCAGGTTGCGAGTT+GGG + chr8.1:23732356-23732375 MS.gene60370:intron 45.0%
GCAACTCATTAACCATCCAC+TGG + chr8.1:23732105-23732124 MS.gene60370:intron 45.0%
GCTTCCAGTCCTAATCTACT+AGG + chr8.1:23734157-23734176 MS.gene60370:intron 45.0%
GGCAGAGCAGAAAAAAATCG+AGG + chr8.1:23732877-23732896 MS.gene60370:CDS 45.0%
GTGTTCAAATTTCCAGGTGG+AGG + chr8.1:23731821-23731840 MS.gene60370:CDS 45.0%
GTTCAGTTTGCATTGGAACG+AGG + chr8.1:23732447-23732466 MS.gene60370:CDS 45.0%
TCAGTTTGCATTGGAACGAG+GGG + chr8.1:23732449-23732468 MS.gene60370:CDS 45.0%
TCATTGGATCACCCACCATT+AGG + chr8.1:23734106-23734125 MS.gene60370:intron 45.0%
TCCACTGTCAGATGCTTGTA+AGG - chr8.1:23731764-23731783 None:intergenic 45.0%
TCCTTACAAGCATCTGACAG+TGG + chr8.1:23731760-23731779 MS.gene60370:CDS 45.0%
TCTAGCCGAAAGTCTTTGCT+GGG + chr8.1:23732901-23732920 MS.gene60370:CDS 45.0%
TGATCGGCACCACTAGATAT+CGG + chr8.1:23733411-23733430 MS.gene60370:CDS 45.0%
TGATTATGCAGGTTGCGAGT+TGG + chr8.1:23732355-23732374 MS.gene60370:intron 45.0%
TGGTACTGTCAGAACAGCAT+TGG + chr8.1:23731910-23731929 MS.gene60370:CDS 45.0%
TTGTGGTTAATTGTGCCCAG+TGG - chr8.1:23732124-23732143 None:intergenic 45.0%
TTTAATGCTTACTGGCCCAC+TGG + chr8.1:23732263-23732282 MS.gene60370:intron 45.0%
! AGCTATGTCATTTGCTCCGA+AGG + chr8.1:23732407-23732426 MS.gene60370:CDS 45.0%
! CACTAACCATTTTCAGCCCA+TGG - chr8.1:23732564-23732583 None:intergenic 45.0%
! CGCAATTTTTCCTCTTGAGG+TGG - chr8.1:23731659-23731678 None:intergenic 45.0%
! CTCTAGGGAATGTCATTGCT+CGG - chr8.1:23731607-23731626 None:intergenic 45.0%
! TAATCACGGCTTTTAGGCCA+TGG - chr8.1:23731722-23731741 None:intergenic 45.0%
! TGCAGAGGGCATGTATCTAA+TGG + chr8.1:23732748-23732767 MS.gene60370:intron 45.0%
! TTCACTTTGGCTGGTTACCT+CGG - chr8.1:23733234-23733253 None:intergenic 45.0%
!! GTATAGGAAGCTGACTTGCA+AGG - chr8.1:23733636-23733655 None:intergenic 45.0%
!!! GTTGATCGAGTTTTAAGGCC+TGG + chr8.1:23732773-23732792 MS.gene60370:CDS 45.0%
!!! AAATAGTAATATTTTATTTT+TGG - chr8.1:23733903-23733922 None:intergenic 5.0%
AAAGGAGTCGTGTACCCTTC+GGG - chr8.1:23731698-23731717 None:intergenic 50.0%
AACATGGAAGCGAACGAAGG+AGG + chr8.1:23732847-23732866 MS.gene60370:CDS 50.0%
ACATGGCAAAGCAGTGGTTC+CGG + chr8.1:23731392-23731411 MS.gene60370:CDS 50.0%
ACCAGCCAAAGTGAAGTTGC+CGG + chr8.1:23733239-23733258 MS.gene60370:CDS 50.0%
ATGGACATGAATGCAGGCCT+TGG + chr8.1:23733440-23733459 MS.gene60370:CDS 50.0%
CAAAGCAGTGGTTCCGGAAA+GGG + chr8.1:23731398-23731417 MS.gene60370:CDS 50.0%
CAAGCATCTGACAGTGGAGA+AGG + chr8.1:23731766-23731785 MS.gene60370:CDS 50.0%
CCAAACATGGAAGCGAACGA+AGG + chr8.1:23732844-23732863 MS.gene60370:CDS 50.0%
CCTTCGTTCGCTTCCATGTT+TGG - chr8.1:23732847-23732866 None:intergenic 50.0%
CGGATTATGACCGATCCTTC+TGG - chr8.1:23734307-23734326 None:intergenic 50.0%
GACCGCATATTAAGGCCAGA+AGG + chr8.1:23734289-23734308 MS.gene60370:CDS 50.0%
GATAGCAGGAGACCTAATGG+TGG - chr8.1:23734121-23734140 None:intergenic 50.0%
GGTTAATTGTGCCCAGTGGA+TGG - chr8.1:23732120-23732139 None:intergenic 50.0%
TCATTGCTCGGTCTTGCTCT+TGG - chr8.1:23731595-23731614 None:intergenic 50.0%
TCCGATAGCAGGAGACCTAA+TGG - chr8.1:23734124-23734143 None:intergenic 50.0%
TTGGGAACATGGCAAAGCAG+TGG + chr8.1:23731386-23731405 MS.gene60370:CDS 50.0%
! ACAGCATTGGACACTGGTTG+CGG + chr8.1:23731923-23731942 MS.gene60370:CDS 50.0%
! CGGCTTTTAGGCCATGGAAA+AGG - chr8.1:23731716-23731735 None:intergenic 50.0%
! CTCGTGATGGGAATGGAAGA+CGG - chr8.1:23732506-23732525 None:intergenic 50.0%
! GGTACACGACTCCTTTTCCA+TGG + chr8.1:23731702-23731721 MS.gene60370:CDS 50.0%
! GTCAGAACAGCATTGGACAC+TGG + chr8.1:23731917-23731936 MS.gene60370:CDS 50.0%
! TCAAAGGCTCGTGATGGGAA+TGG - chr8.1:23732513-23732532 None:intergenic 50.0%
! TCACTTTGGCTGGTTACCTC+GGG - chr8.1:23733233-23733252 None:intergenic 50.0%
!! AAGGCATTGTCCACCTCAAG+AGG + chr8.1:23731646-23731665 MS.gene60370:CDS 50.0%
!! ATATACGCATCGGCTCCTTG+AGG - chr8.1:23731857-23731876 None:intergenic 50.0%
!! CGAGGGGTACCTGCTATTAT+TGG + chr8.1:23732465-23732484 MS.gene60370:CDS 50.0%
!! TATACGCATCGGCTCCTTGA+GGG - chr8.1:23731856-23731875 None:intergenic 50.0%
!!! GATCGAGTTTTAAGGCCTGG+TGG + chr8.1:23732776-23732795 MS.gene60370:CDS 50.0%
AAGGAAGTAAACCGGGCCAG+TGG - chr8.1:23732282-23732301 None:intergenic 55.0%
AGCCAAAGTGAAGTTGCCGG+AGG + chr8.1:23733242-23733261 MS.gene60370:CDS 55.0%
AGGAAGTAAACCGGGCCAGT+GGG - chr8.1:23732281-23732300 None:intergenic 55.0%
AGGGCGATATCGCTATCTGG+AGG + chr8.1:23732939-23732958 MS.gene60370:CDS 55.0%
CTCCTCCGGCAACTTCACTT+TGG - chr8.1:23733247-23733266 None:intergenic 55.0%
GACATGAATGCAGGCCTTGG+AGG + chr8.1:23733443-23733462 MS.gene60370:CDS 55.0%
GCAAAGCAGTGGTTCCGGAA+AGG + chr8.1:23731397-23731416 MS.gene60370:CDS 55.0%
GCGAACGAAGGAGGATCTGA+AGG + chr8.1:23732856-23732875 MS.gene60370:CDS 55.0%
GGAGGAACCATGTTCCCTCA+AGG + chr8.1:23731839-23731858 MS.gene60370:CDS 55.0%
GGGAGACAGCCTTGCATTGA+AGG + chr8.1:23731418-23731437 MS.gene60370:CDS 55.0%
GTGCAGCTGCAAATCCTCCA+AGG - chr8.1:23733460-23733479 None:intergenic 55.0%
TCCGGCAACTTCACTTTGGC+TGG - chr8.1:23733243-23733262 None:intergenic 55.0%
TCTCCTGCTATCGGACCAGT+TGG + chr8.1:23734129-23734148 MS.gene60370:intron 55.0%
TGCAAGGCTGTCTCCCTTTC+CGG - chr8.1:23731414-23731433 None:intergenic 55.0%
! AGCATTGGACACTGGTTGCG+GGG + chr8.1:23731925-23731944 MS.gene60370:CDS 55.0%
! ATCGGCTCCTTGAGGGAACA+TGG - chr8.1:23731849-23731868 None:intergenic 55.0%
! CACTTTGGCTGGTTACCTCG+GGG - chr8.1:23733232-23733251 None:intergenic 55.0%
! CAGCATTGGACACTGGTTGC+GGG + chr8.1:23731924-23731943 MS.gene60370:CDS 55.0%
ACCCTTCGGGAGCAGGAATG+AGG - chr8.1:23731685-23731704 None:intergenic 60.0%
CCTTACCGGGCAACAGAGCA+CGG + chr8.1:23731312-23731331 MS.gene60370:CDS 60.0%
CTGCAAAACCCCTATCCCCG+AGG + chr8.1:23733214-23733233 MS.gene60370:CDS 60.0%
GACTGGAAGCCTGAGCCAAC+TGG - chr8.1:23734147-23734166 None:intergenic 60.0%
GAGCCAACTGGTCCGATAGC+AGG - chr8.1:23734135-23734154 None:intergenic 60.0%
GCCTCATTCCTGCTCCCGAA+GGG + chr8.1:23731681-23731700 MS.gene60370:CDS 60.0%
GCTCGGTCTTGCTCTTGGCA+AGG - chr8.1:23731590-23731609 None:intergenic 60.0%
GCTGGTTACCTCGGGGATAG+GGG - chr8.1:23733225-23733244 None:intergenic 60.0%
GGGAACATGGTTCCTCCACC+TGG - chr8.1:23731836-23731855 None:intergenic 60.0%
GGGACTGCAAACAGCCTAGC+AGG - chr8.1:23733284-23733303 None:intergenic 60.0%
TGCCTCATTCCTGCTCCCGA+AGG + chr8.1:23731680-23731699 MS.gene60370:CDS 60.0%
! GCTATCGGACCAGTTGGCTC+AGG + chr8.1:23734135-23734154 MS.gene60370:intron 60.0%
! GGCTGGTTACCTCGGGGATA+GGG - chr8.1:23733226-23733245 None:intergenic 60.0%
! TGGCTGGTTACCTCGGGGAT+AGG - chr8.1:23733227-23733246 None:intergenic 60.0%
CCGTGCTCTGTTGCCCGGTA+AGG - chr8.1:23731315-23731334 None:intergenic 65.0%
GTCGTGTACCCTTCGGGAGC+AGG - chr8.1:23731692-23731711 None:intergenic 65.0%
TACCGGGCAACAGAGCACGG+AGG + chr8.1:23731315-23731334 MS.gene60370:CDS 65.0%
TGCTTACTGGCCCACTGGCC+CGG + chr8.1:23732268-23732287 MS.gene60370:intron 65.0%
GGCCTCCGTGCTCTGTTGCC+CGG - chr8.1:23731320-23731339 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 23731296 23734489 23731296 ID=MS.gene60370
chr8.1 mRNA 23731296 23734489 23731296 ID=MS.gene60370.t1;Parent=MS.gene60370
chr8.1 exon 23731296 23731946 23731296 ID=MS.gene60370.t1.exon1;Parent=MS.gene60370.t1
chr8.1 CDS 23731296 23731946 23731296 ID=cds.MS.gene60370.t1;Parent=MS.gene60370.t1
chr8.1 exon 23732366 23732576 23732366 ID=MS.gene60370.t1.exon2;Parent=MS.gene60370.t1
chr8.1 CDS 23732366 23732576 23732366 ID=cds.MS.gene60370.t1;Parent=MS.gene60370.t1
chr8.1 exon 23732753 23733035 23732753 ID=MS.gene60370.t1.exon3;Parent=MS.gene60370.t1
chr8.1 CDS 23732753 23733035 23732753 ID=cds.MS.gene60370.t1;Parent=MS.gene60370.t1
chr8.1 exon 23733196 23733582 23733196 ID=MS.gene60370.t1.exon4;Parent=MS.gene60370.t1
chr8.1 CDS 23733196 23733582 23733196 ID=cds.MS.gene60370.t1;Parent=MS.gene60370.t1
chr8.1 exon 23733738 23733815 23733738 ID=MS.gene60370.t1.exon5;Parent=MS.gene60370.t1
chr8.1 CDS 23733738 23733815 23733738 ID=cds.MS.gene60370.t1;Parent=MS.gene60370.t1
chr8.1 exon 23734258 23734489 23734258 ID=MS.gene60370.t1.exon6;Parent=MS.gene60370.t1
chr8.1 CDS 23734258 23734489 23734258 ID=cds.MS.gene60370.t1;Parent=MS.gene60370.t1
Gene Sequence

>MS.gene60370

ATGGGTTCTAAAAATGCCTTACCGGGCAACAGAGCACGGAGGCCTTTGTCAATTTTCGCTGTGCTTGCTCTGTGTTGTTTGTTTTATCTTTTGGGAACATGGCAAAGCAGTGGTTCCGGAAAGGGAGACAGCCTTGCATTGAAGGTAAATAAGATGCAGGCAACAACAGACTGCAACATTGTACCGAATTTGAATTTTGAACCCCAACACAAATATGTAGAAATTGTTGAGCAATCCGAACCGAAAGCTAAAATGTTCAAGGCATGTGATGTAAAATATACTGATTATACACCTTGCCAAGAGCAAGACCGAGCAATGACATTCCCTAGAGAAAACATGATCTATAGGGAAAGGCATTGTCCACCTCAAGAGGAAAAATTGCGTTGCCTCATTCCTGCTCCCGAAGGGTACACGACTCCTTTTCCATGGCCTAAAAGCCGTGATTATGCCTACTATGCTAATGTTCCTTACAAGCATCTGACAGTGGAGAAGGCTAATCAAAACTGGGTGAAATTTGAAGGGAATGTGTTCAAATTTCCAGGTGGAGGAACCATGTTCCCTCAAGGAGCCGATGCGTATATTGATGAGCTTGCATCGGTTATTCCAATCAAAGATGGTACTGTCAGAACAGCATTGGACACTGGTTGCGGGGTAAGCTTTCATGTCAAATAATCCTTTTGATATAGTATGTTATTTTTCTTTGTTGAGAAATATTTCGACACACAAATTTGACATCAAATTTGATGGTTACTATTCAGATGTGTGGTTAATTACGTTTAGTTGATGATTAATGAATATAACAAGGTGTAGCAACTCATTAACCATCCACTGGGCACAATTAACCACAATTTTCAAATGTGTTTAATTATGTGTAATGTATAATGGGGTTTTGTCTATTGGATTCCAAATTTCTCAATTTTCAGTGTATTTAGTATACTGAAGTCGGAATCATTTGTCCTTTCTTTGTTTTAATGCTTACTGGCCCACTGGCCCGGTTTACTTCCTTTCAGTATGCACCTTTCAATTGCTTCTTGATTGTTGTAGCAATATAAGCGTGAATGATTATGCAGGTTGCGAGTTGGGGAGCGTACTTGCTAAAGAGAAATGTGCTAGCTATGTCATTTGCTCCGAAGGATAACCATGAAGCACAAGTTCAGTTTGCATTGGAACGAGGGGTACCTGCTATTATTGGTGTTCTTGGTTCGATCCGTCTTCCATTCCCATCACGAGCCTTTGATATGTCTCAGTGTTCTCGTTGTCTAATACCATGGGCTGAAAATGGTTAGTGCTAAATTAGACAGTCAATAAGATTGATCTAGAATACAGTATTATCGGCATTTTCTTATGTCGATGATGATATTCCTTAACTTCTAAATATAATCATGCAAATGTTAATAATTCTGAAAATCTGAATGCAACATTGTTTAGTTGATTTTTCACCCAATAATTGAATGCAGAGGGCATGTATCTAATGGAAGTTGATCGAGTTTTAAGGCCTGGTGGATTTTGGATTTTATCTGGCCCTCCAATTAATTGGAAAACGTACTACCAAACATGGAAGCGAACGAAGGAGGATCTGAAGGCAGAGCAGAAAAAAATCGAGGATCTAGCCGAAAGTCTTTGCTGGGAAAAGAAGTATGAAAAGGGCGATATCGCTATCTGGAGGAAGAAAATAAATGCTAAATCATGTCAAAGAAAATCTCCCAATTTATGTGATTTAGATAATGCTGATGATGTTTGGTAAGCCCTGATCATTTCTACACATTTCATTTAACAAGTATAATGATTCATTTGTTGTTAGAGAGATTTGAATAATTATTTGTTCATATAATTCCACGATTTCGGATTATAATGCGCTCATGAATAACTTTTCTCCATGCTCCAAAATTTGTAATGGTAGGTACAAAAAAATGGAGGTCTGCAAAACCCCTATCCCCGAGGTAACCAGCCAAAGTGAAGTTGCCGGAGGAGAATTGAAGAAGTTTCCTGCTAGGCTGTTTGCAGTCCCTCCTAGAATAGCTAAAGACGTTATTCCAGGCGTAACAGTTGATTCTTATCAAGAGGACAACAAATTATGGAAAAAGCGTGTTAATAATTACAAAAGAATTAATAGATTGATCGGCACCACTAGATATCGGAATGTAATGGACATGAATGCAGGCCTTGGAGGATTTGCAGCTGCACTTGAATCACAAAAATCTTGGGTGATGAATGCTGTGCCCACAATTGCTGACAACACTTTAGGTGTTATCTATGAAAGAGGTCTCATTGGTATTTATCATGACTGGTAAGTTTCTAAAATTATCTTCATAAAGTTTTCTAATTTTATGATGATTCCCTTGCAAGTCAGCTTCCTATACTCTAAAATGATTAATTAAACTGATATTTATGGATATTTTGTTATGGTATGAAATTGCAATGACATTCGTTTTCTTTCTTCAGGTGTGAAGGCTTTTCTACATATCCAAGGACATATGATCTTATTCATGCCAATGGTTTATTTAGTTTGTACCAGGACAAGTAAGTTTCTTCCTTTTCCATCCACAAGTGATCATAGTGATAATTGGTAATTTGAAATTATAACTATATCAAGTCTTTTACTTACCAAAAATAAAATATTACTATTTTTACTGATTTTTCCAATTCTGGTATAATTGAGACTATAATTTGTTTGCCCCACATTTGATAAGATTAGACCTGAAAATATGTTTATAAGTGGTAGACAATCCTCACCTTACAAGCTGATTTTGTAAGAGTTAAGTTAAAGCTCGACCTAAATTTTAAGATGGTATCAAAGTCTATTCAAGATTTCATTGGATCACCCACCATTAGGTCTCCTGCTATCGGACCAGTTGGCTCAGGCTTCCAGTCCTAATCTACTAGGAAAAAAAAACAGAGAACTTCAACATGTGATTATAATTCTGACTTAATTTAACATAAGTATTGTTGATTTGATTGACAGGTGCAACCTAGAAGACATCCTTTTAGAGATGGACCGCATATTAAGGCCAGAAGGATCGGTCATAATCCGCGACGAAGTTGATGTGATTAACAAGGTTCAGAAAATTGTTAGGGGAATGAGATGGGAATCTAAAATGGTGGATCATGAGGATGGCCCATTTGCACCCGAGAAGATATTGGTTATTGTAAAAGAGTATTGGGTTGGCAGCAGTAAAAACAACACATCCAGTTAG
Protein sequence

>MS.gene60370.t1

MGSKNALPGNRARRPLSIFAVLALCCLFYLLGTWQSSGSGKGDSLALKVNKMQATTDCNIVPNLNFEPQHKYVEIVEQSEPKAKMFKACDVKYTDYTPCQEQDRAMTFPRENMIYRERHCPPQEEKLRCLIPAPEGYTTPFPWPKSRDYAYYANVPYKHLTVEKANQNWVKFEGNVFKFPGGGTMFPQGADAYIDELASVIPIKDGTVRTALDTGCGVASWGAYLLKRNVLAMSFAPKDNHEAQVQFALERGVPAIIGVLGSIRLPFPSRAFDMSQCSRCLIPWAENEGMYLMEVDRVLRPGGFWILSGPPINWKTYYQTWKRTKEDLKAEQKKIEDLAESLCWEKKYEKGDIAIWRKKINAKSCQRKSPNLCDLDNADDVWYKKMEVCKTPIPEVTSQSEVAGGELKKFPARLFAVPPRIAKDVIPGVTVDSYQEDNKLWKKRVNNYKRINRLIGTTRYRNVMDMNAGLGGFAAALESQKSWVMNAVPTIADNTLGVIYERGLIGIYHDWCEGFSTYPRTYDLIHANGLFSLYQDKCNLEDILLEMDRILRPEGSVIIRDEVDVINKVQKIVRGMRWESKMVDHEDGPFAPEKILVIVKEYWVGSSKNNTSS