Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034054.t1 | XP_024638013.1 | 95.3 | 106 | 5 | 0 | 1 | 106 | 1 | 106 | 7.70E-48 | 199.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034054.t1 | Q9SSC1 | 41.5 | 142 | 43 | 6 | 1 | 106 | 1 | 138 | 3.3e-15 | 82.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034054.t1 | A0A396HYZ6 | 95.3 | 106 | 5 | 0 | 1 | 106 | 1 | 106 | 5.6e-48 | 199.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene034054 | MS.gene86369 | PPI |
| MS.gene36231 | MS.gene034054 | PPI |
| MS.gene36229 | MS.gene034054 | PPI |
| MS.gene89049 | MS.gene034054 | PPI |
| MS.gene060879 | MS.gene034054 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034054.t1 | MTR_4g011060 | 95.238 | 105 | 5 | 0 | 1 | 105 | 1 | 105 | 4.98e-69 | 202 |
| MS.gene034054.t1 | MTR_2g103380 | 56.780 | 118 | 39 | 1 | 1 | 106 | 1 | 118 | 8.79e-39 | 126 |
| MS.gene034054.t1 | MTR_4g114050 | 53.191 | 94 | 32 | 4 | 1 | 93 | 1 | 83 | 2.86e-23 | 86.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034054.t1 | AT1G80180 | 41.549 | 142 | 43 | 6 | 1 | 106 | 1 | 138 | 2.62e-23 | 87.4 |
| MS.gene034054.t1 | AT5G20100 | 47.959 | 98 | 47 | 2 | 3 | 96 | 2 | 99 | 5.49e-22 | 83.6 |
| MS.gene034054.t1 | AT1G15400 | 39.394 | 132 | 46 | 6 | 1 | 103 | 1 | 127 | 2.78e-19 | 77.4 |
| MS.gene034054.t1 | AT1G15400 | 39.394 | 132 | 46 | 6 | 1 | 103 | 1 | 127 | 3.02e-19 | 77.0 |
| MS.gene034054.t1 | AT1G15400 | 39.394 | 132 | 46 | 6 | 1 | 103 | 1 | 127 | 3.58e-19 | 77.4 |
Find 30 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGGTTCATCTGGTCTTGTT+TGG | 0.224185 | 4.4:+88270402 | MS.gene034054:CDS |
| AGGTTCATCTGGTCTTGTTT+GGG | 0.315565 | 4.4:+88270403 | MS.gene034054:CDS |
| TGTGGATTCTGTAGCCCTTT+TGG | 0.323362 | 4.4:+88270614 | MS.gene034054:CDS |
| CTACCTCGACCGACGTTTAC+CGG | 0.339840 | 4.4:-88270660 | None:intergenic |
| TGGGATGACAGATTCTTATC+AGG | 0.348639 | 4.4:+88270422 | MS.gene034054:CDS |
| CTGAATGATACTTCAGATCT+TGG | 0.351403 | 4.4:-88270374 | None:intergenic |
| GGAGACGGTGGTTCGATCGC+TGG | 0.391781 | 4.4:-88270578 | None:intergenic |
| TTCAGAAGATCAGGTTCATC+TGG | 0.432719 | 4.4:+88270392 | MS.gene034054:CDS |
| GAAGTATCATTCAGAAGATC+AGG | 0.445781 | 4.4:+88270383 | MS.gene034054:CDS |
| AGAAGCCATTCCACCGGTGC+TGG | 0.447002 | 4.4:+88270527 | MS.gene034054:CDS |
| GGTGGAATGGCTTCTTTGTA+CGG | 0.453050 | 4.4:-88270519 | None:intergenic |
| GAGACGGTGGTTCGATCGCT+GGG | 0.462717 | 4.4:-88270577 | None:intergenic |
| CTCGTCCAGCACCGGTGGAA+TGG | 0.498060 | 4.4:-88270532 | None:intergenic |
| TCTCCGAAGGTTTCTGCGTG+TGG | 0.512115 | 4.4:+88270596 | MS.gene034054:CDS |
| CATTCCACCGGTGCTGGACG+AGG | 0.514080 | 4.4:+88270533 | MS.gene034054:CDS |
| TACCTCGACCGACGTTTACC+GGG | 0.526089 | 4.4:-88270659 | None:intergenic |
| AATCCACACGCAGAAACCTT+CGG | 0.536395 | 4.4:-88270599 | None:intergenic |
| ACGGTATCCTCGTCCAGCAC+CGG | 0.568784 | 4.4:-88270540 | None:intergenic |
| AGCCCGGTAAACGTCGGTCG+AGG | 0.570758 | 4.4:+88270657 | MS.gene034054:CDS |
| CACGCAGAAACCTTCGGAGA+CGG | 0.583247 | 4.4:-88270593 | None:intergenic |
| AAGGGTCAACGATCAAAGCC+CGG | 0.598386 | 4.4:+88270641 | MS.gene034054:CDS |
| GATCGAACCACCGTCTCCGA+AGG | 0.606120 | 4.4:+88270583 | MS.gene034054:CDS |
| CGGTAAACGTCGGTCGAGGT+AGG | 0.609038 | 4.4:+88270661 | MS.gene034054:CDS |
| GGAATGGCTTCTTTGTACGG+TGG | 0.613872 | 4.4:-88270516 | None:intergenic |
| GTATCCTCGTCCAGCACCGG+TGG | 0.623334 | 4.4:-88270537 | None:intergenic |
| GCTGGACGAGGATACCGTAC+CGG | 0.652103 | 4.4:+88270545 | MS.gene034054:CDS |
| GCAGAAACCTTCGGAGACGG+TGG | 0.660406 | 4.4:-88270590 | None:intergenic |
| AGACGGTGGTTCGATCGCTG+GGG | 0.689604 | 4.4:-88270576 | None:intergenic |
| GTACAAAGAAGCCATTCCAC+CGG | 0.692582 | 4.4:+88270521 | MS.gene034054:CDS |
| GATCAAAGCCCGGTAAACGT+CGG | 0.721418 | 4.4:+88270651 | MS.gene034054:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTGAATGATACTTCAGATCT+TGG | - | chr4.4:88270377-88270396 | None:intergenic | 35.0% | |
| ! | GAAGTATCATTCAGAAGATC+AGG | + | chr4.4:88270383-88270402 | MS.gene034054:CDS | 35.0% |
| ! | GGTTTTCACATGAAGTTCTT+TGG | - | chr4.4:88270498-88270517 | None:intergenic | 35.0% |
| !! | TGTAGCCCTTTTGGAAAAAA+GGG | + | chr4.4:88270623-88270642 | MS.gene034054:CDS | 35.0% |
| !!! | GTTGACCCTTTTTTCCAAAA+GGG | - | chr4.4:88270631-88270650 | None:intergenic | 35.0% |
| TGGGATGACAGATTCTTATC+AGG | + | chr4.4:88270422-88270441 | MS.gene034054:CDS | 40.0% | |
| TTCAGAAGATCAGGTTCATC+TGG | + | chr4.4:88270392-88270411 | MS.gene034054:CDS | 40.0% | |
| !! | AGGTTCATCTGGTCTTGTTT+GGG | + | chr4.4:88270403-88270422 | MS.gene034054:CDS | 40.0% |
| !! | CTGTAGCCCTTTTGGAAAAA+AGG | + | chr4.4:88270622-88270641 | MS.gene034054:CDS | 40.0% |
| !!! | CGTTGACCCTTTTTTCCAAA+AGG | - | chr4.4:88270632-88270651 | None:intergenic | 40.0% |
| AATCCACACGCAGAAACCTT+CGG | - | chr4.4:88270602-88270621 | None:intergenic | 45.0% | |
| GGTGGAATGGCTTCTTTGTA+CGG | - | chr4.4:88270522-88270541 | None:intergenic | 45.0% | |
| GTACAAAGAAGCCATTCCAC+CGG | + | chr4.4:88270521-88270540 | MS.gene034054:CDS | 45.0% | |
| ! | TGTGGATTCTGTAGCCCTTT+TGG | + | chr4.4:88270614-88270633 | MS.gene034054:CDS | 45.0% |
| !! | CAGGTTCATCTGGTCTTGTT+TGG | + | chr4.4:88270402-88270421 | MS.gene034054:CDS | 45.0% |
| AAGGGTCAACGATCAAAGCC+CGG | + | chr4.4:88270641-88270660 | MS.gene034054:CDS | 50.0% | |
| GATCAAAGCCCGGTAAACGT+CGG | + | chr4.4:88270651-88270670 | MS.gene034054:CDS | 50.0% | |
| GGAATGGCTTCTTTGTACGG+TGG | - | chr4.4:88270519-88270538 | None:intergenic | 50.0% | |
| CACGCAGAAACCTTCGGAGA+CGG | - | chr4.4:88270596-88270615 | None:intergenic | 55.0% | |
| CTACCTCGACCGACGTTTAC+CGG | - | chr4.4:88270663-88270682 | None:intergenic | 55.0% | |
| TACCTCGACCGACGTTTACC+GGG | - | chr4.4:88270662-88270681 | None:intergenic | 55.0% | |
| TCTCCGAAGGTTTCTGCGTG+TGG | + | chr4.4:88270596-88270615 | MS.gene034054:CDS | 55.0% | |
| ! | CTGGGGAGACTTTTCCGGTA+CGG | - | chr4.4:88270562-88270581 | None:intergenic | 55.0% |
| ! | GATCGCTGGGGAGACTTTTC+CGG | - | chr4.4:88270567-88270586 | None:intergenic | 55.0% |
| ACGGTATCCTCGTCCAGCAC+CGG | - | chr4.4:88270543-88270562 | None:intergenic | 60.0% | |
| AGAAGCCATTCCACCGGTGC+TGG | + | chr4.4:88270527-88270546 | MS.gene034054:CDS | 60.0% | |
| AGACGGTGGTTCGATCGCTG+GGG | - | chr4.4:88270579-88270598 | None:intergenic | 60.0% | |
| GAGACGGTGGTTCGATCGCT+GGG | - | chr4.4:88270580-88270599 | None:intergenic | 60.0% | |
| GATCGAACCACCGTCTCCGA+AGG | + | chr4.4:88270583-88270602 | MS.gene034054:CDS | 60.0% | |
| GCAGAAACCTTCGGAGACGG+TGG | - | chr4.4:88270593-88270612 | None:intergenic | 60.0% | |
| GCTGGACGAGGATACCGTAC+CGG | + | chr4.4:88270545-88270564 | MS.gene034054:CDS | 60.0% | |
| AGCCCGGTAAACGTCGGTCG+AGG | + | chr4.4:88270657-88270676 | MS.gene034054:CDS | 65.0% | |
| GGAGACGGTGGTTCGATCGC+TGG | - | chr4.4:88270581-88270600 | None:intergenic | 65.0% | |
| ! | CATTCCACCGGTGCTGGACG+AGG | + | chr4.4:88270533-88270552 | MS.gene034054:CDS | 65.0% |
| ! | GTATCCTCGTCCAGCACCGG+TGG | - | chr4.4:88270540-88270559 | None:intergenic | 65.0% |
| !! | CTCGTCCAGCACCGGTGGAA+TGG | - | chr4.4:88270535-88270554 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 88270362 | 88270682 | 88270362 | ID=MS.gene034054 |
| chr4.4 | mRNA | 88270362 | 88270682 | 88270362 | ID=MS.gene034054.t1;Parent=MS.gene034054 |
| chr4.4 | exon | 88270362 | 88270682 | 88270362 | ID=MS.gene034054.t1.exon1;Parent=MS.gene034054.t1 |
| chr4.4 | CDS | 88270362 | 88270682 | 88270362 | ID=cds.MS.gene034054.t1;Parent=MS.gene034054.t1 |
| Gene Sequence |
| Protein sequence |
