AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034244

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034244.t1 MTR_4g006940 98.810 336 4 0 1 336 1 336 0.0 689
MS.gene034244.t1 MTR_4g006940 99.286 280 2 0 1 280 1 280 0.0 574
MS.gene034244.t1 MTR_4g006940 99.286 280 2 0 1 280 1 280 0.0 574
MS.gene034244.t1 MTR_2g104960 81.155 329 59 2 7 335 12 337 0.0 536
MS.gene034244.t1 MTR_3g005170 51.242 322 148 6 9 323 2 321 2.76e-105 311
MS.gene034244.t1 MTR_3g005210 50.159 315 148 6 16 323 5 317 6.79e-104 308
MS.gene034244.t1 MTR_4g077100 48.125 320 157 6 11 323 5 322 1.23e-96 289
MS.gene034244.t1 MTR_5g072620 46.519 316 162 3 15 324 3 317 2.45e-96 288
MS.gene034244.t1 MTR_8g062440 47.531 324 160 6 10 326 1 321 1.52e-95 286
MS.gene034244.t1 MTR_4g077130 47.500 320 159 7 11 323 3 320 8.83e-95 285
MS.gene034244.t1 MTR_4g009040 46.296 324 162 4 11 326 4 323 1.35e-94 284
MS.gene034244.t1 MTR_4g009040 45.732 328 162 4 11 326 4 327 5.64e-94 283
MS.gene034244.t1 MTR_8g062440 47.385 325 160 7 10 326 1 322 1.49e-93 281
MS.gene034244.t1 MTR_8g062110 47.352 321 159 6 10 323 11 328 4.63e-93 280
MS.gene034244.t1 MTR_8g062110 47.352 321 159 6 10 323 2 319 4.66e-93 280
MS.gene034244.t1 MTR_3g005170 51.254 279 128 5 9 280 2 279 7.23e-91 273
MS.gene034244.t1 MTR_4g009690 46.914 324 160 4 11 326 4 323 1.26e-88 269
MS.gene034244.t1 MTR_3g005170 46.541 318 138 6 9 323 2 290 2.02e-88 267
MS.gene034244.t1 MTR_4g009690 47.222 324 158 5 11 326 4 322 3.79e-88 268
MS.gene034244.t1 MTR_4g077100 49.458 277 132 5 11 280 5 280 1.62e-84 257
MS.gene034244.t1 MTR_3g031650 42.089 316 176 3 15 324 3 317 1.65e-83 256
MS.gene034244.t1 MTR_8g062440 48.571 280 135 5 10 282 1 278 4.50e-82 252
MS.gene034244.t1 MTR_8g062110 47.842 278 136 5 10 280 2 277 6.41e-81 248
MS.gene034244.t1 MTR_8g062110 47.842 278 136 5 10 280 2 277 7.25e-81 248
MS.gene034244.t1 MTR_5g056600 41.456 316 177 5 18 326 5 319 4.04e-80 247
MS.gene034244.t1 MTR_1g022440 41.411 326 173 11 15 327 6 326 1.87e-76 238
MS.gene034244.t1 MTR_3g031650 44.569 267 142 2 15 275 3 269 3.64e-76 235
MS.gene034244.t1 MTR_2g028620 41.176 323 177 6 14 326 7 326 3.59e-75 234
MS.gene034244.t1 MTR_2g101330 39.506 324 185 5 17 332 5 325 1.02e-74 233
MS.gene034244.t1 MTR_1g022445 41.159 328 171 12 15 327 6 326 2.37e-73 230
MS.gene034244.t1 MTR_2g028620 42.336 274 146 5 14 277 7 278 4.96e-64 206
MS.gene034244.t1 MTR_4g092080 35.385 325 189 9 18 324 15 336 5.32e-54 180
MS.gene034244.t1 MTR_7g074880 33.333 327 201 9 13 326 4 326 5.52e-54 180
MS.gene034244.t1 MTR_8g062110 41.964 224 125 4 102 323 13 233 1.20e-52 174
MS.gene034244.t1 MTR_7g074820 33.837 331 197 12 13 326 4 329 1.61e-51 174
MS.gene034244.t1 MTR_7g074730 33.435 329 200 10 13 326 4 328 2.77e-51 173
MS.gene034244.t1 MTR_4g081440 34.328 335 190 10 18 327 21 350 1.28e-49 169
MS.gene034244.t1 MTR_4g081440 34.821 336 188 11 18 327 25 355 1.66e-49 169
MS.gene034244.t1 MTR_7g074850 32.432 333 199 12 13 326 4 329 6.53e-48 164
MS.gene034244.t1 MTR_4g023730 33.846 325 197 10 17 326 8 329 2.45e-44 155
MS.gene034244.t1 MTR_7g074710 32.622 328 202 12 13 326 4 326 4.53e-44 154
MS.gene034244.t1 MTR_8g062110 41.436 181 102 3 102 280 13 191 8.35e-41 141
MS.gene034244.t1 MTR_6g406250 34.333 300 178 8 13 299 51 344 4.14e-40 144
MS.gene034244.t1 MTR_0685s0030 41.875 160 91 2 164 323 2 159 1.84e-37 132
MS.gene034244.t1 MTR_7g074870 30.000 290 186 10 50 326 3 288 7.33e-34 126
MS.gene034244.t1 MTR_5g029990 30.449 312 192 10 2 306 3 296 1.25e-33 125
MS.gene034244.t1 MTR_2g013250 27.219 338 217 10 17 328 7 341 2.40e-28 112
MS.gene034244.t1 MTR_6g406250 33.491 212 128 5 13 213 51 260 2.17e-26 105
MS.gene034244.t1 MTR_7g117190 27.059 340 212 13 17 326 7 340 2.87e-26 107
MS.gene034244.t1 MTR_0164s0070 43.262 141 61 4 17 138 5 145 3.68e-26 103
MS.gene034244.t1 MTR_2g013230 27.874 348 202 13 17 328 8 342 3.75e-26 107
MS.gene034244.t1 MTR_0685s0030 42.735 117 66 1 164 280 2 117 1.00e-25 99.8
MS.gene034244.t1 MTR_2g013260 26.393 341 208 11 17 324 8 338 2.61e-25 104
MS.gene034244.t1 MTR_7g117190 28.620 297 180 12 17 284 7 300 5.65e-25 103
MS.gene034244.t1 MTR_2g013260 26.689 296 178 9 17 280 8 296 2.48e-23 98.2
MS.gene034244.t1 MTR_3g011130 41.667 108 60 3 108 212 35 142 2.19e-18 80.9
MS.gene034244.t1 MTR_8g062710 48.750 80 31 1 17 96 20 89 1.54e-17 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034244.t1 AT1G80820 73.968 315 82 0 15 329 6 320 2.96e-171 479
MS.gene034244.t1 AT1G15950 72.871 317 86 0 10 326 6 322 3.26e-167 469
MS.gene034244.t1 AT1G15950 72.871 317 86 0 10 326 6 322 3.26e-167 469
MS.gene034244.t1 AT1G15950 72.168 309 86 0 10 318 6 314 1.18e-159 450
MS.gene034244.t1 AT1G80820 73.308 266 71 0 15 280 6 271 3.98e-138 393
MS.gene034244.t1 AT1G15950 72.222 270 75 0 11 280 7 276 1.57e-136 389
MS.gene034244.t1 AT1G15950 71.533 274 78 0 11 284 7 280 1.83e-136 389
MS.gene034244.t1 AT1G15950 72.119 269 75 0 11 279 7 275 9.31e-136 387
MS.gene034244.t1 AT1G15950 75.893 224 54 0 103 326 1 224 7.11e-115 333
MS.gene034244.t1 AT5G19440 51.090 321 148 6 11 324 4 322 3.45e-105 311
MS.gene034244.t1 AT1G51410 48.457 324 156 7 11 326 3 323 3.23e-98 293
MS.gene034244.t1 AT5G58490 44.025 318 171 3 15 326 7 323 3.44e-87 265
MS.gene034244.t1 AT1G66800 47.385 325 150 8 10 323 1 315 1.05e-86 264
MS.gene034244.t1 AT1G09480 44.037 327 168 7 10 326 1 322 4.66e-86 262
MS.gene034244.t1 AT2G33590 47.436 312 154 5 17 322 9 316 1.21e-85 261
MS.gene034244.t1 AT1G09480 44.037 327 168 7 10 326 48 369 1.28e-85 263
MS.gene034244.t1 AT1G09510 45.031 322 166 7 10 323 1 319 3.15e-85 260
MS.gene034244.t1 AT1G09490 44.410 322 168 7 13 326 4 322 9.41e-85 259
MS.gene034244.t1 AT2G02400 41.509 318 177 4 15 326 4 318 9.18e-83 254
MS.gene034244.t1 AT1G09500 44.000 325 168 8 10 323 1 322 2.88e-81 250
MS.gene034244.t1 AT1G76470 43.614 321 171 3 15 327 5 323 4.05e-80 247
MS.gene034244.t1 AT4G35420 42.587 317 172 7 17 326 8 321 9.72e-80 246
MS.gene034244.t1 AT1G68540 40.379 317 179 5 19 326 6 321 7.01e-75 234
MS.gene034244.t1 AT1G09500 46.182 275 135 7 10 274 1 272 1.34e-74 231
MS.gene034244.t1 AT2G33600 45.513 312 160 5 17 322 9 316 1.20e-73 230
MS.gene034244.t1 AT1G25460 36.825 315 191 4 19 326 6 319 1.10e-66 213
MS.gene034244.t1 AT5G42800 39.264 326 182 10 15 328 6 327 7.08e-66 213
MS.gene034244.t1 AT1G09490 41.135 282 156 6 52 326 13 291 2.89e-64 205
MS.gene034244.t1 AT1G09500 41.667 288 154 8 47 323 4 288 2.38e-61 198
MS.gene034244.t1 AT2G45400 35.976 328 191 10 16 326 39 364 5.05e-53 179
MS.gene034244.t1 AT1G61720 34.084 311 185 9 13 306 9 316 6.78e-51 172
MS.gene034244.t1 AT2G45400 37.276 279 160 7 16 279 39 317 4.97e-49 167
MS.gene034244.t1 AT4G27250 35.647 317 171 12 18 306 14 325 1.31e-47 164
MS.gene034244.t1 AT4G27250 35.922 309 171 12 18 306 14 315 5.56e-47 162
MS.gene034244.t1 AT1G68540 37.441 211 130 2 117 326 5 214 3.26e-46 156
MS.gene034244.t1 AT2G23910 32.441 299 176 8 18 302 11 297 6.25e-44 153
MS.gene034244.t1 AT2G23910 32.971 276 160 7 18 280 11 274 7.22e-44 152
MS.gene034244.t1 AT2G23910 33.212 274 158 7 18 278 11 272 1.26e-43 151
MS.gene034244.t1 AT4G30470 30.952 294 186 6 18 302 11 296 1.21e-38 139
MS.gene034244.t1 AT2G45400 32.653 294 180 9 49 326 16 307 1.39e-35 131
MS.gene034244.t1 AT5G14700 33.213 277 161 8 12 270 50 320 1.70e-35 132
MS.gene034244.t1 AT2G23910 31.679 262 154 7 54 302 14 263 1.14e-34 128
MS.gene034244.t1 AT2G45400 37.037 216 122 6 16 217 39 254 5.29e-34 125
MS.gene034244.t1 AT5G14700 32.444 225 132 6 12 221 50 269 9.07e-27 107
MS.gene034244.t1 AT4G33360 28.279 244 152 8 17 253 15 242 1.10e-17 82.8
MS.gene034244.t1 AT4G33360 28.279 244 152 8 17 253 3 230 1.37e-17 82.8
MS.gene034244.t1 AT4G33360 28.279 244 152 8 17 253 15 242 1.78e-17 82.4

Find 77 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACATCTCTACTCGTATT+TGG 0.148678 4.1:+88443420 None:intergenic
CTGGCGGGTTTATAGCTTCT+TGG 0.222359 4.1:-88444105 MS.gene034244:CDS
CACATCCTCAAGTACCTTAA+TGG 0.244324 4.1:-88443074 MS.gene034244:CDS
GTGAACCCAGTTGTGGTTCT+TGG 0.275623 4.1:-88443134 MS.gene034244:CDS
TCTTTCAAGTGATCATTCTT+TGG 0.278143 4.1:+88443796 None:intergenic
TGGTGAGGTTGTTGAAATTT+TGG 0.282212 4.1:-88442919 MS.gene034244:CDS
AAAAGAAAATCAAGTTGATT+TGG 0.293241 4.1:-88443162 MS.gene034244:CDS
ATCGATTGGAACGGTCTATA+TGG 0.308610 4.1:-88443445 MS.gene034244:CDS
GAATGATCACTTGAAAGAAT+TGG 0.322640 4.1:-88443791 MS.gene034244:CDS
TTGGCTTGTCAAACTTCTCT+TGG 0.330178 4.1:-88444086 MS.gene034244:CDS
AATATGTATGGTTTCACTCT+TGG 0.331350 4.1:+88441690 None:intergenic
TCTATCCAATCTGTTGTTCA+TGG 0.337063 4.1:-88443715 MS.gene034244:CDS
TATGAGACAAATTCTGCTTC+TGG 0.341001 4.1:-88442975 MS.gene034244:CDS
TTCTTTGTTCCATTCACTGC+TGG 0.346517 4.1:+88443519 None:intergenic
AATGTTTATATGAAACTGTT+AGG 0.352716 4.1:-88441617 MS.gene034244:CDS
TGTTGCATTCACATAAGTTT+TGG 0.352789 4.1:+88443046 None:intergenic
GAAAGAGGTTACACTGTTAG+AGG 0.360156 4.1:-88444064 MS.gene034244:CDS
GTGCAGAACTGGTATTGTTA+TGG 0.378304 4.1:-88443221 MS.gene034244:intron
CAACATCTCTACTCGTATTT+GGG 0.380185 4.1:+88443421 None:intergenic
AAATTTGCATACCTGGGTTT+CGG 0.388841 4.1:+88444035 None:intergenic
TCTTCTTGCATGGGAGGAAT+AGG 0.388850 4.1:+88441570 None:intergenic
ACAATCTTATGTTCATGTTA+AGG 0.400887 4.1:-88443024 MS.gene034244:CDS
ATCGTGTGTGTCACCGGTGC+TGG 0.407046 4.1:-88444124 MS.gene034244:CDS
CACCGGTGACACACACGATT+TGG 0.409017 4.1:+88444128 None:intergenic
TGCAGAACTGGTATTGTTAT+GGG 0.425363 4.1:-88443220 MS.gene034244:intron
TTAATTGTTGGTTGAAGTAA+TGG 0.425863 4.1:+88443110 None:intergenic
TCATATAAACATTGCTTCAC+AGG 0.433601 4.1:+88441627 None:intergenic
AATGGTCCAAGAACCACAAC+TGG 0.433883 4.1:+88443128 None:intergenic
ATTGTACTAGCATTAATTGT+TGG 0.463159 4.1:+88443098 None:intergenic
GTCAAACTTCTCTTGGAAAG+AGG 0.467940 4.1:-88444079 MS.gene034244:CDS
AAAATAACTCACAGGGTTGT+CGG 0.470932 4.1:+88443657 None:intergenic
AGAGAGGCTAACTTTGCATA+AGG 0.471199 4.1:-88443758 MS.gene034244:CDS
AAATCAAAAGCTGAAAGACT+TGG 0.471600 4.1:-88441664 MS.gene034244:CDS
GATAACACTTCATCAGTCTC+TGG 0.493312 4.1:-88444157 MS.gene034244:CDS
GACTGATGAAGTGTTATCAT+AGG 0.499014 4.1:+88444162 None:intergenic
CATATAATAGGATGAGATGT+TGG 0.502572 4.1:-88443544 MS.gene034244:intron
AACACTTACTTGGTAGGAAG+TGG 0.504048 4.1:+88442876 None:intergenic
TTATGTTCATGTTAAGGATG+TGG 0.505241 4.1:-88443018 MS.gene034244:CDS
GTTGTGTTCACTTCATCGAT+TGG 0.505971 4.1:-88443459 MS.gene034244:CDS
AGCTATAAACCCGCCAGCAC+CGG 0.506486 4.1:+88444111 None:intergenic
AAAGCTGAAAGACTTGGGAT+TGG 0.510048 4.1:-88441658 MS.gene034244:CDS
CTCACAGGGTTGTCGGTGAC+AGG 0.510560 4.1:+88443664 None:intergenic
GACCAAATCGTGTGTGTCAC+CGG 0.524328 4.1:-88444130 MS.gene034244:CDS
TTGTAGTTGATGAATCGTAT+TGG 0.525667 4.1:-88443401 MS.gene034244:CDS
GGCAACCATGAACAACAGAT+TGG 0.526807 4.1:+88443710 None:intergenic
TTAAGCAGAGTCTTCTTGCA+TGG 0.532426 4.1:+88441560 None:intergenic
ATGTTGGAGCCAGCAGTGAA+TGG 0.533675 4.1:-88443528 MS.gene034244:CDS
TGTGTGTCACCGGTGCTGGC+GGG 0.540247 4.1:-88444120 MS.gene034244:CDS
GATGAAGTGTTATCATAGGC+AGG 0.541028 4.1:+88444166 None:intergenic
GTGTGTGTCACCGGTGCTGG+CGG 0.544302 4.1:-88444121 MS.gene034244:CDS
AATCAAAAGCTGAAAGACTT+GGG 0.547219 4.1:-88441663 MS.gene034244:CDS
TTGGTAGGAAGTGGGTACTC+AGG 0.551005 4.1:+88442885 None:intergenic
TCTGTTGTTCATGGTTGCCA+TGG 0.552494 4.1:-88443706 MS.gene034244:CDS
ATATGAAACACTTACTTGGT+AGG 0.553678 4.1:+88442870 None:intergenic
TAAGCAGAGTCTTCTTGCAT+GGG 0.557099 4.1:+88441561 None:intergenic
AGAGGAACCGTCCGAAACCC+AGG 0.563011 4.1:-88444046 MS.gene034244:intron
GTTAGGAGTCTACAAGAGAA+AGG 0.565801 4.1:-88441600 MS.gene034244:CDS
GGTTGTTGTGAACCCAGTTG+TGG 0.566597 4.1:-88443141 MS.gene034244:CDS
TGGGTACTCAGGAAAATACT+TGG 0.568031 4.1:+88442896 None:intergenic
GCAGAGTCTTCTTGCATGGG+AGG 0.572180 4.1:+88441564 None:intergenic
GATCACTTGAAAGAATTGGA+AGG 0.576295 4.1:-88443787 MS.gene034244:CDS
CAGCACCATTAAGGTACTTG+AGG 0.581125 4.1:+88443069 None:intergenic
TTGCATACCTGGGTTTCGGA+CGG 0.587038 4.1:+88444039 None:intergenic
GTTCACTTCATCGATTGGAA+CGG 0.592538 4.1:-88443454 MS.gene034244:CDS
AACTGCTTTGCATCGTGGTG+AGG 0.603860 4.1:-88442934 MS.gene034244:CDS
CAGTGGCTGAACAATCAGCA+TGG 0.627131 4.1:-88443193 MS.gene034244:CDS
AGTGGCTGAACAATCAGCAT+GGG 0.640259 4.1:-88443192 MS.gene034244:CDS
AACACTTCATCAGTCTCTGG+TGG 0.644313 4.1:-88444154 MS.gene034244:CDS
CTTGCATGGGAGGAATAGGA+AGG 0.645331 4.1:+88441574 None:intergenic
AATTGGAAGGAGCAAGAGAG+AGG 0.645670 4.1:-88443774 MS.gene034244:CDS
ACACTTACTTGGTAGGAAGT+GGG 0.647055 4.1:+88442877 None:intergenic
AAGCTGTGTGGAACACACCA+TGG 0.653770 4.1:+88443689 None:intergenic
ATGGTCCAAGAACCACAACT+GGG 0.677277 4.1:+88443129 None:intergenic
TGTGAAACTGCTTTGCATCG+TGG 0.688310 4.1:-88442939 MS.gene034244:CDS
AGAGCATTGCAAGAACACCA+AGG 0.704040 4.1:-88443370 MS.gene034244:intron
CGGTGACAGGAGAAGCTGTG+TGG 0.708743 4.1:+88443677 None:intergenic
GTATTGTTATGGGAAGACAG+TGG 0.831616 4.1:-88443210 MS.gene034244:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTTATGATATATTCT+TGG + chr4.1:88443653-88443672 None:intergenic 10.0%
!! ATTAAGAAAAATTTATTGAT+TGG - chr4.1:88443559-88443578 MS.gene034244:intron 10.0%
!! CATTTAGTTAATTAATATAA+GGG - chr4.1:88443496-88443515 MS.gene034244:CDS 10.0%
!! GTTAAGTTAAATAAATAAAA+TGG - chr4.1:88441824-88441843 MS.gene034244:intron 10.0%
!!! TTAAGAAAAATTTATTGATT+GGG - chr4.1:88443560-88443579 MS.gene034244:intron 10.0%
!!! TTTTTAAGTTAATTATGTTA+TGG - chr4.1:88442895-88442914 MS.gene034244:CDS 10.0%
!! CATAATTTAACAAATACTTT+GGG - chr4.1:88443930-88443949 MS.gene034244:intron 15.0%
!! GCATTTAGTTAATTAATATA+AGG - chr4.1:88443495-88443514 MS.gene034244:CDS 15.0%
!! TATTAATTAACTAAATGCTT+AGG + chr4.1:88443493-88443512 None:intergenic 15.0%
!! TGACTTAATTATAAATTCTA+AGG + chr4.1:88443327-88443346 None:intergenic 15.0%
!! TGTGAAATTAAATTTACAAA+TGG + chr4.1:88442107-88442126 None:intergenic 15.0%
!! TTTATAAAGAGTAACTTATA+AGG - chr4.1:88442141-88442160 MS.gene034244:intron 15.0%
!!! AGTATATTAGAACTTTTAAT+TGG + chr4.1:88443535-88443554 None:intergenic 15.0%
!!! ATATATCATAAATAATTTGC+TGG - chr4.1:88443656-88443675 MS.gene034244:intron 15.0%
!! AAATAATTGCTATGTTTATG+TGG - chr4.1:88441762-88441781 MS.gene034244:intron 20.0%
!! AATGTTTATATGAAACTGTT+AGG - chr4.1:88444112-88444131 MS.gene034244:CDS 20.0%
!! AGATGAACATATCATATATT+GGG - chr4.1:88443616-88443635 MS.gene034244:intron 20.0%
!! GCATAATTTAACAAATACTT+TGG - chr4.1:88443929-88443948 MS.gene034244:intron 20.0%
!! TAGATGAACATATCATATAT+TGG - chr4.1:88443615-88443634 MS.gene034244:intron 20.0%
!!! AAAAGAAAATCAAGTTGATT+TGG - chr4.1:88442567-88442586 MS.gene034244:intron 20.0%
!!! AAGGTGAATTAATCATTTTA+TGG - chr4.1:88442160-88442179 MS.gene034244:intron 20.0%
!!! AAGTCTTAAATTAAGTTGTT+TGG - chr4.1:88443106-88443125 MS.gene034244:CDS 20.0%
!!! AATTATAAATTCTAAGGAGT+AGG + chr4.1:88443321-88443340 None:intergenic 20.0%
!!! ACTTTTGATCTCAAAATTTT+GGG + chr4.1:88443090-88443109 None:intergenic 20.0%
!!! ATTGAGTTTTTTTGTTTGTT+AGG + chr4.1:88443229-88443248 None:intergenic 20.0%
!!! CATTTTATGGAACATATAAT+AGG - chr4.1:88442173-88442192 MS.gene034244:intron 20.0%
!!! GTGAATTTTTGTATAATTAG+AGG - chr4.1:88441908-88441927 MS.gene034244:intron 20.0%
!!! TATTAGAACTTTTAATTGGA+TGG + chr4.1:88443531-88443550 None:intergenic 20.0%
!!! TTGAGTTTTTTTGTTTGTTA+GGG + chr4.1:88443228-88443247 None:intergenic 20.0%
!!! TTTTGATTTGAGAATATGTA+TGG + chr4.1:88444054-88444073 None:intergenic 20.0%
! AAACATAGAAAATAACTCAC+AGG + chr4.1:88442083-88442102 None:intergenic 25.0%
! AACATAGAAAATAACTCACA+GGG + chr4.1:88442082-88442101 None:intergenic 25.0%
! ACAATCTTATGTTCATGTTA+AGG - chr4.1:88442705-88442724 MS.gene034244:intron 25.0%
! ATGATATATTCTTGGTTAGT+AGG + chr4.1:88443645-88443664 None:intergenic 25.0%
! ATTACATTAAGAGTCAAAGT+TGG + chr4.1:88443294-88443313 None:intergenic 25.0%
! ATTCATCATAGCAATAGAAA+CGG + chr4.1:88443844-88443863 None:intergenic 25.0%
! ATTGTACTAGCATTAATTGT+TGG + chr4.1:88442634-88442653 None:intergenic 25.0%
! TAGATGTACACATTGAATAA+GGG + chr4.1:88443132-88443151 None:intergenic 25.0%
! TCTATTGCTATGATGAATTA+GGG - chr4.1:88443846-88443865 MS.gene034244:intron 25.0%
! TTAGATGTACACATTGAATA+AGG + chr4.1:88443133-88443152 None:intergenic 25.0%
! TTCTATTGCTATGATGAATT+AGG - chr4.1:88443845-88443864 MS.gene034244:intron 25.0%
! TTTAAAGAAACATGCACTTA+AGG + chr4.1:88443684-88443703 None:intergenic 25.0%
!! ACTTAAGTCAATGATTTTAC+AGG - chr4.1:88443367-88443386 MS.gene034244:intron 25.0%
!! CTTTTGATCTCAAAATTTTG+GGG + chr4.1:88443089-88443108 None:intergenic 25.0%
!! GACTTTTGATCTCAAAATTT+TGG + chr4.1:88443091-88443110 None:intergenic 25.0%
!! TTAATTGTTGGTTGAAGTAA+TGG + chr4.1:88442622-88442641 None:intergenic 25.0%
!!! AATTTTTTTTTGTGCAGAAC+TGG - chr4.1:88442497-88442516 MS.gene034244:intron 25.0%
!!! GCTAAAATTTTGAAAGTGAA+AGG + chr4.1:88443973-88443992 None:intergenic 25.0%
AAATCAAAAGCTGAAAGACT+TGG - chr4.1:88444065-88444084 MS.gene034244:CDS 30.0%
AATATGTATGGTTTCACTCT+TGG + chr4.1:88444042-88444061 None:intergenic 30.0%
AATCAAAAGCTGAAAGACTT+GGG - chr4.1:88444066-88444085 MS.gene034244:CDS 30.0%
ATATGAAACACTTACTTGGT+AGG + chr4.1:88442862-88442881 None:intergenic 30.0%
ATGACTTCTCCTAAAAATAG+TGG + chr4.1:88443435-88443454 None:intergenic 30.0%
CATATAATAGGATGAGATGT+TGG - chr4.1:88442185-88442204 MS.gene034244:intron 30.0%
CTTCTGACTTATCTAGATTA+GGG - chr4.1:88442935-88442954 MS.gene034244:CDS 30.0%
GAAAAAAACTTAGTTGAGAG+AGG + chr4.1:88442980-88442999 None:intergenic 30.0%
GAATGATCACTTGAAAGAAT+TGG - chr4.1:88441938-88441957 MS.gene034244:intron 30.0%
GACTATATGAAACACTTACT+TGG + chr4.1:88442866-88442885 None:intergenic 30.0%
GTAGTGAATTAATCAAACCT+TGG + chr4.1:88442379-88442398 None:intergenic 30.0%
TCATATAAACATTGCTTCAC+AGG + chr4.1:88444105-88444124 None:intergenic 30.0%
TGTACATCTAACTTGATCTT+GGG - chr4.1:88443142-88443161 MS.gene034244:CDS 30.0%
TTAAGTGTCTTGCATATATG+AGG - chr4.1:88443907-88443926 MS.gene034244:intron 30.0%
TTATGTTCATGTTAAGGATG+TGG - chr4.1:88442711-88442730 MS.gene034244:intron 30.0%
! ATTTTACAGGTAAAAACGAG+TGG - chr4.1:88443380-88443399 MS.gene034244:CDS 30.0%
! TCTTTCAAGTGATCATTCTT+TGG + chr4.1:88441936-88441955 None:intergenic 30.0%
! TGTTGCATTCACATAAGTTT+TGG + chr4.1:88442686-88442705 None:intergenic 30.0%
!! TGACTTGTTCATTCTAAACA+GGG + chr4.1:88443188-88443207 None:intergenic 30.0%
!! TTGACTTGTTCATTCTAAAC+AGG + chr4.1:88443189-88443208 None:intergenic 30.0%
!! TTGTAGTTGATGAATCGTAT+TGG - chr4.1:88442328-88442347 MS.gene034244:intron 30.0%
AAAATAACTCACAGGGTTGT+CGG + chr4.1:88442075-88442094 None:intergenic 35.0%
AAACACCAATTGATGCAGAA+AGG - chr4.1:88443252-88443271 MS.gene034244:intron 35.0%
AAATTTGCATACCTGGGTTT+CGG + chr4.1:88441697-88441716 None:intergenic 35.0%
ACAACATCTCTACTCGTATT+TGG + chr4.1:88442312-88442331 None:intergenic 35.0%
CAACATCTCTACTCGTATTT+GGG + chr4.1:88442311-88442330 None:intergenic 35.0%
GATCACTTGAAAGAATTGGA+AGG - chr4.1:88441942-88441961 MS.gene034244:intron 35.0%
GCTTCTGACTTATCTAGATT+AGG - chr4.1:88442934-88442953 MS.gene034244:CDS 35.0%
GTACATCTAACTTGATCTTG+GGG - chr4.1:88443143-88443162 MS.gene034244:CDS 35.0%
GTGTACATCTAACTTGATCT+TGG - chr4.1:88443141-88443160 MS.gene034244:CDS 35.0%
TAATCTATAGTCGCAGAGAT+AGG + chr4.1:88443812-88443831 None:intergenic 35.0%
TATGAGACAAATTCTGCTTC+TGG - chr4.1:88442754-88442773 MS.gene034244:intron 35.0%
TCTATCCAATCTGTTGTTCA+TGG - chr4.1:88442014-88442033 MS.gene034244:intron 35.0%
TGCAGAACTGGTATTGTTAT+GGG - chr4.1:88442509-88442528 MS.gene034244:intron 35.0%
! GACTGATGAAGTGTTATCAT+AGG + chr4.1:88441570-88441589 None:intergenic 35.0%
! TGGTGAGGTTGTTGAAATTT+TGG - chr4.1:88442810-88442829 MS.gene034244:intron 35.0%
!! CATTACCAGCCACTATTTTT+AGG - chr4.1:88443423-88443442 MS.gene034244:CDS 35.0%
!! CGATATTTGTTAACGCGTTT+TGG - chr4.1:88443777-88443796 MS.gene034244:CDS 35.0%
!! GAACTCGAGATTTTGTTTAC+AGG - chr4.1:88443013-88443032 MS.gene034244:CDS 35.0%
AAAGCTGAAAGACTTGGGAT+TGG - chr4.1:88444071-88444090 MS.gene034244:CDS 40.0%
AACACTTACTTGGTAGGAAG+TGG + chr4.1:88442856-88442875 None:intergenic 40.0%
ACACTTACTTGGTAGGAAGT+GGG + chr4.1:88442855-88442874 None:intergenic 40.0%
ACCAATTGATGCAGAAAGGT+GGG - chr4.1:88443256-88443275 MS.gene034244:intron 40.0%
AGAGAGGCTAACTTTGCATA+AGG - chr4.1:88441971-88441990 MS.gene034244:intron 40.0%
ATCGATTGGAACGGTCTATA+TGG - chr4.1:88442284-88442303 MS.gene034244:intron 40.0%
ATCTAACTTGATCTTGGGGT+GGG - chr4.1:88443147-88443166 MS.gene034244:CDS 40.0%
CACATCCTCAAGTACCTTAA+TGG - chr4.1:88442655-88442674 MS.gene034244:intron 40.0%
CTTCTCCTAAAAATAGTGGC+TGG + chr4.1:88443431-88443450 None:intergenic 40.0%
GAAAGAGGTTACACTGTTAG+AGG - chr4.1:88441665-88441684 MS.gene034244:CDS 40.0%
GATAACACTTCATCAGTCTC+TGG - chr4.1:88441572-88441591 MS.gene034244:CDS 40.0%
GTATTGTTATGGGAAGACAG+TGG - chr4.1:88442519-88442538 MS.gene034244:intron 40.0%
GTCAAACTTCTCTTGGAAAG+AGG - chr4.1:88441650-88441669 MS.gene034244:CDS 40.0%
GTGCAGAACTGGTATTGTTA+TGG - chr4.1:88442508-88442527 MS.gene034244:intron 40.0%
GTGTGTGACAATACAATGGT+TGG + chr4.1:88443460-88443479 None:intergenic 40.0%
GTGTGTGTGTGACAATACAA+TGG + chr4.1:88443464-88443483 None:intergenic 40.0%
GTTAGGAGTCTACAAGAGAA+AGG - chr4.1:88444129-88444148 MS.gene034244:CDS 40.0%
GTTGTGTTCACTTCATCGAT+TGG - chr4.1:88442270-88442289 MS.gene034244:intron 40.0%
TAAGCAGAGTCTTCTTGCAT+GGG + chr4.1:88444171-88444190 None:intergenic 40.0%
TGGGTACTCAGGAAAATACT+TGG + chr4.1:88442836-88442855 None:intergenic 40.0%
TTAAGCAGAGTCTTCTTGCA+TGG + chr4.1:88444172-88444191 None:intergenic 40.0%
TTCTTTGTTCCATTCACTGC+TGG + chr4.1:88442213-88442232 None:intergenic 40.0%
! GATGAAGTGTTATCATAGGC+AGG + chr4.1:88441566-88441585 None:intergenic 40.0%
! GTTCACTTCATCGATTGGAA+CGG - chr4.1:88442275-88442294 MS.gene034244:intron 40.0%
!! AAGTTTTGGCAGCACCATTA+AGG + chr4.1:88442672-88442691 None:intergenic 40.0%
!! GTCAAAGTTGGTATACCTAG+AGG + chr4.1:88443282-88443301 None:intergenic 40.0%
!! TTGGCTTGTCAAACTTCTCT+TGG - chr4.1:88441643-88441662 MS.gene034244:CDS 40.0%
AACACTTCATCAGTCTCTGG+TGG - chr4.1:88441575-88441594 MS.gene034244:CDS 45.0%
AATGGTCCAAGAACCACAAC+TGG + chr4.1:88442604-88442623 None:intergenic 45.0%
AATTGGAAGGAGCAAGAGAG+AGG - chr4.1:88441955-88441974 MS.gene034244:intron 45.0%
AGTGGCTGAACAATCAGCAT+GGG - chr4.1:88442537-88442556 MS.gene034244:intron 45.0%
ATGGTCCAAGAACCACAACT+GGG + chr4.1:88442603-88442622 None:intergenic 45.0%
CACCAATTGATGCAGAAAGG+TGG - chr4.1:88443255-88443274 MS.gene034244:intron 45.0%
CATCTAACTTGATCTTGGGG+TGG - chr4.1:88443146-88443165 MS.gene034244:CDS 45.0%
CGCGACAAATTTGCATACCT+GGG + chr4.1:88441703-88441722 None:intergenic 45.0%
CTTAGTTGAGAGAGGAGAGT+TGG + chr4.1:88442972-88442991 None:intergenic 45.0%
GGCAACCATGAACAACAGAT+TGG + chr4.1:88442022-88442041 None:intergenic 45.0%
GTCGCAGAGATAGGTACAAT+TGG + chr4.1:88443803-88443822 None:intergenic 45.0%
TCGCGACAAATTTGCATACC+TGG + chr4.1:88441704-88441723 None:intergenic 45.0%
TCTAACTTGATCTTGGGGTG+GGG - chr4.1:88443148-88443167 MS.gene034244:CDS 45.0%
TCTGTTGTTCATGGTTGCCA+TGG - chr4.1:88442023-88442042 MS.gene034244:intron 45.0%
TCTTCTTGCATGGGAGGAAT+AGG + chr4.1:88444162-88444181 None:intergenic 45.0%
TGTGAAACTGCTTTGCATCG+TGG - chr4.1:88442790-88442809 MS.gene034244:intron 45.0%
! CAGCACCATTAAGGTACTTG+AGG + chr4.1:88442663-88442682 None:intergenic 45.0%
!! AGAGCATTGCAAGAACACCA+AGG - chr4.1:88442359-88442378 MS.gene034244:intron 45.0%
!! AATATTAAGATTAATTAATT+AGG - chr4.1:88442460-88442479 MS.gene034244:intron 5.0%
AACTGCTTTGCATCGTGGTG+AGG - chr4.1:88442795-88442814 MS.gene034244:intron 50.0%
AAGCTGTGTGGAACACACCA+TGG + chr4.1:88442043-88442062 None:intergenic 50.0%
ATGTTGGAGCCAGCAGTGAA+TGG - chr4.1:88442201-88442220 MS.gene034244:intron 50.0%
CAGTGGCTGAACAATCAGCA+TGG - chr4.1:88442536-88442555 MS.gene034244:intron 50.0%
CTTGCATGGGAGGAATAGGA+AGG + chr4.1:88444158-88444177 None:intergenic 50.0%
GACCAAATCGTGTGTGTCAC+CGG - chr4.1:88441599-88441618 MS.gene034244:CDS 50.0%
GCCCACCTTTCTGCATCAAT+TGG + chr4.1:88443260-88443279 None:intergenic 50.0%
GGTTGTTGTGAACCCAGTTG+TGG - chr4.1:88442588-88442607 MS.gene034244:intron 50.0%
GTGAACCCAGTTGTGGTTCT+TGG - chr4.1:88442595-88442614 MS.gene034244:intron 50.0%
TTGCATACCTGGGTTTCGGA+CGG + chr4.1:88441693-88441712 None:intergenic 50.0%
TTGGTAGGAAGTGGGTACTC+AGG + chr4.1:88442847-88442866 None:intergenic 50.0%
!! CTGGCGGGTTTATAGCTTCT+TGG - chr4.1:88441624-88441643 MS.gene034244:CDS 50.0%
AACTTGATCTTGGGGTGGGG+TGG - chr4.1:88443151-88443170 MS.gene034244:CDS 55.0%
ACTTGATCTTGGGGTGGGGT+GGG - chr4.1:88443152-88443171 MS.gene034244:CDS 55.0%
AGCTATAAACCCGCCAGCAC+CGG + chr4.1:88441621-88441640 None:intergenic 55.0%
ATGCAGAAAGGTGGGCCTCT+AGG - chr4.1:88443264-88443283 MS.gene034244:intron 55.0%
CACCGGTGACACACACGATT+TGG + chr4.1:88441604-88441623 None:intergenic 55.0%
GCAGAGTCTTCTTGCATGGG+AGG + chr4.1:88444168-88444187 None:intergenic 55.0%
AGAGGAACCGTCCGAAACCC+AGG - chr4.1:88441683-88441702 MS.gene034244:CDS 60.0%
ATCGTGTGTGTCACCGGTGC+TGG - chr4.1:88441605-88441624 MS.gene034244:CDS 60.0%
CGGTGACAGGAGAAGCTGTG+TGG + chr4.1:88442055-88442074 None:intergenic 60.0%
CTCACAGGGTTGTCGGTGAC+AGG + chr4.1:88442068-88442087 None:intergenic 60.0%
! GTGTGTGTCACCGGTGCTGG+CGG - chr4.1:88441608-88441627 MS.gene034244:CDS 65.0%
!! TGTGTGTCACCGGTGCTGGC+GGG - chr4.1:88441609-88441628 MS.gene034244:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 88441560 88444191 88441560 ID=MS.gene034244
chr4.1 mRNA 88441560 88444191 88441560 ID=MS.gene034244.t1;Parent=MS.gene034244
chr4.1 exon 88444047 88444191 88444047 ID=MS.gene034244.t1.exon1;Parent=MS.gene034244.t1
chr4.1 CDS 88444047 88444191 88444047 ID=cds.MS.gene034244.t1;Parent=MS.gene034244.t1
chr4.1 exon 88443669 88443823 88443669 ID=MS.gene034244.t1.exon2;Parent=MS.gene034244.t1
chr4.1 CDS 88443669 88443823 88443669 ID=cds.MS.gene034244.t1;Parent=MS.gene034244.t1
chr4.1 exon 88443371 88443556 88443371 ID=MS.gene034244.t1.exon3;Parent=MS.gene034244.t1
chr4.1 CDS 88443371 88443556 88443371 ID=cds.MS.gene034244.t1;Parent=MS.gene034244.t1
chr4.1 exon 88442885 88443237 88442885 ID=MS.gene034244.t1.exon4;Parent=MS.gene034244.t1
chr4.1 CDS 88442885 88443237 88442885 ID=cds.MS.gene034244.t1;Parent=MS.gene034244.t1
chr4.1 exon 88441560 88441731 88441560 ID=MS.gene034244.t1.exon5;Parent=MS.gene034244.t1
chr4.1 CDS 88441560 88441731 88441560 ID=cds.MS.gene034244.t1;Parent=MS.gene034244.t1
Gene Sequence

>MS.gene034244

ATGCCTGCCTATGATAACACTTCATCAGTCTCTGGTGGCGACCAAATCGTGTGTGTCACCGGTGCTGGCGGGTTTATAGCTTCTTGGCTTGTCAAACTTCTCTTGGAAAGAGGTTACACTGTTAGAGGAACCGTCCGAAACCCAGGTATGCAAATTTGTCGCGATAAGTAGCATTACTCTAAAAATCAAGTATAAGAAAATGAAATAATTGCTATGTTTATGTGGTTACATAACATAACATGACATAATATGTATTTCTTGCATGTTAAGTTAAATAAATAAAATGGTAGCTTTATTAATTTCATGTTTAATTGAAATTTATGTTATTTCTGACTTGAGTATGCATGTGTGAATTTTTGTATAATTAGAGGATCCAAAGAATGATCACTTGAAAGAATTGGAAGGAGCAAGAGAGAGGCTAACTTTGCATAAGGTTGATCTTCTTGATCTTCAATCTATCCAATCTGTTGTTCATGGTTGCCATGGTGTGTTCCACACAGCTTCTCCTGTCACCGACAACCCTGTGAGTTATTTTCTATGTTTTCCATTTGTAAATTTAATTTCACATAGAAAATGTAACATTTATAAAGAGTAACTTATAAGGTGAATTAATCATTTTATGGAACATATAATAGGATGAGATGTTGGAGCCAGCAGTGAATGGAACAAAGAATGTGATTATAGCGTCTGCTGAAGCAAAAGTGCGACGTGTTGTGTTCACTTCATCGATTGGAACGGTCTATATGGACCCAAATACGAGTAGAGATGTTGTAGTTGATGAATCGTATTGGAGTGATTTAGAGCATTGCAAGAACACCAAGGTTTGATTAATTCACTACTTTAATAGTATTTACTATAATAGAAAATAATAGTGTGATATCATAACTTAATGAGAATATTAATATTAAGATTAATTAATTAGGTGATTAATAATTTTAATTTTTTTTTGTGCAGAACTGGTATTGTTATGGGAAGACAGTGGCTGAACAATCAGCATGGGACATAGCAAAAGAAAATCAAGTTGATTTGGTTGTTGTGAACCCAGTTGTGGTTCTTGGACCATTACTTCAACCAACAATTAATGCTAGTACAATTCACATCCTCAAGTACCTTAATGGTGCTGCCAAAACTTATGTGAATGCAACACAATCTTATGTTCATGTTAAGGATGTGGCATTAGCACATCTTCTTGTTTATGAGACAAATTCTGCTTCTGGTAGATACATATGTTGTGAAACTGCTTTGCATCGTGGTGAGGTTGTTGAAATTTTGGCCAAGTATTTTCCTGAGTACCCACTTCCTACCAAGTAAGTGTTTCATATAGTCAAATTGTCTTTTTTAAGTTAATTATGTTATGGTGCGAAATATATATATGCTTCTGACTTATCTAGATTAGGGTATCTGTCAGACCAACTCTCCTCTCTCAACTAAGTTTTTTTCATCGACAAAATTTGAACTCGAGATTTTGTTTACAGGATCAAAGTGAAGTTTTACTCAGACTATTATTATACATAGTTAAAAAATTTCCCCAAAATTTTGAGATCAAAAGTCTTAAATTAAGTTGTTTGGCCCTTATTCAATGTGTACATCTAACTTGATCTTGGGGTGGGGTGGGAAAACTAGTTCCCTGTTTAGAATGAACAAGTCAAGTATTTTCAATGTGTTCCCTAACAAACAAAAAAACTCAATGTGAAACACCAATTGATGCAGAAAGGTGGGCCTCTAGGTATACCAACTTTGACTCTTAATGTAATATTTCCTACTCCTTAGAATTTATAATTAAGTCAATTTAAATTCAAGCTTCTCAACTTAAGTCAATGATTTTACAGGTAAAAACGAGTGGAGAGTACAAGTTGTATAAGTCATTACCAGCCACTATTTTTAGGAGAAGTCATTACCAACCATTGTATTGTCACACACACACACTTTACCTAAGCATTTAGTTAATTAATATAAGGGAGTGCTTAACCATCCAATTAAAAGTTCTAATATACTTTATATTAAGAAAAATTTATTGATTGGGTAAAGTAAAGTGACTTGCTTTGAAAACTTGTTTAGATGAACATATCATATATTGGGAAACCTACTAACCAAGAATATATCATAAATAATTTGCTGGTGCCTTAAGTGCATGTTTCTTTAAATAAAATATTTTTAGAACAACAACAAGTAATAAAAGATTAAATAAGAGAACAAAAGTAAAAGATAATGAACACACGATATTTGTTAACGCGTTTTGGCCAATTGTACCTATCTCTGCGACTATAGATTAGCTATAGTTCCGTTTCTATTGCTATGATGAATTAGGGTTTTAATGTTACGAGCAATATATATAGTACTACTACGATTAAGTGTCTTGCATATATGAGGCATAATTTAACAAATACTTTGGGTTTGAGAAAAGAATATGCCTTTCACTTTCAAAATTTTAGCTAAACATAATTTTTTTTTTCTTTGCAGATGTTCAGATGAAAAGAATCCAAGAGTGAAACCATACATATTCTCAAATCAAAAGCTGAAAGACTTGGGATTGGAATTCACACCTGTGAAGCAATGTTTATATGAAACTGTTAGGAGTCTACAAGAGAAAGGACACCTTCCTATTCCTCCCATGCAAGAAGACTCTGCTTAA
Protein sequence

>MS.gene034244.t1

MPAYDNTSSVSGGDQIVCVTGAGGFIASWLVKLLLERGYTVRGTVRNPEDPKNDHLKELEGARERLTLHKVDLLDLQSIQSVVHGCHGVFHTASPVTDNPDEMLEPAVNGTKNVIIASAEAKVRRVVFTSSIGTVYMDPNTSRDVVVDESYWSDLEHCKNTKNWYCYGKTVAEQSAWDIAKENQVDLVVVNPVVVLGPLLQPTINASTIHILKYLNGAAKTYVNATQSYVHVKDVALAHLLVYETNSASGRYICCETALHRGEVVEILAKYFPEYPLPTKCSDEKNPRVKPYIFSNQKLKDLGLEFTPVKQCLYETVRSLQEKGHLPIPPMQEDSA