AlfalfaGEDB | MS.gene035678

M. sativa cultivar XinJiangDaYe / MS.gene035678

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
PFAM: PF00643.27 PF06203.17

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035678.t1	XP_003610187.1	81.9	376	11	2	1	320	1	375	9.20E-121	443.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035678.t1	Q9FHH8	42.0	371	148	11	1	320	1	355	1.2e-36	155.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035678.t1	G7JD46	81.9	376	11	2	1	320	1	375	6.7e-121	443.4

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene035678.t1	TF	C2C2-CO-like

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene035678	MS.gene75965	PPI
MS.gene035678	MS.gene62915	PPI
MS.gene035678	MS.gene44781	PPI
MS.gene035678	MS.gene022048	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035678.t1	MTR_4g128930	82.447	376	9	5	1	320	1	375	0.0	606
MS.gene035678.t1	MTR_3g105710	48.036	331	96	10	20	319	4	289	3.33e-84	256
MS.gene035678.t1	MTR_1g013450	43.103	348	114	8	20	319	4	315	2.11e-81	249
MS.gene035678.t1	MTR_7g018170	64.835	91	32	0	20	110	24	114	2.98e-39	142
MS.gene035678.t1	MTR_7g018170	58.427	89	29	1	232	320	316	396	6.81e-29	114
MS.gene035678.t1	MTR_3g034680	79.487	78	16	0	67	144	12	89	3.43e-38	131
MS.gene035678.t1	MTR_1g023260	45.161	93	41	1	22	104	5	97	1.32e-20	88.2
MS.gene035678.t1	MTR_1g023260	45.161	93	41	1	22	104	5	97	1.35e-20	90.1
MS.gene035678.t1	MTR_5g069480	43.333	90	51	0	21	110	4	93	6.70e-19	87.0
MS.gene035678.t1	MTR_5g069480	43.333	90	51	0	21	110	4	93	8.18e-19	85.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035678.t1	AT5G57660	47.978	371	126	11	1	320	1	355	1.39e-97	292
MS.gene035678.t1	AT5G24930	44.444	378	114	11	20	320	4	362	6.07e-86	263
MS.gene035678.t1	AT2G24790	51.186	295	90	8	20	287	6	273	3.92e-83	253
MS.gene035678.t1	AT5G15840	35.657	373	165	11	13	320	11	373	9.31e-68	216
MS.gene035678.t1	AT3G02380	36.264	364	139	9	20	320	14	347	1.46e-67	215
MS.gene035678.t1	AT5G15850	38.292	363	156	8	13	320	6	355	2.87e-67	214
MS.gene035678.t1	AT2G24790	52.604	192	58	7	20	210	6	165	5.54e-55	179
MS.gene035678.t1	AT5G15840	36.620	213	120	7	13	212	11	221	7.13e-39	139
MS.gene035678.t1	AT2G33500	44.944	89	49	0	22	110	12	100	1.61e-18	85.9
MS.gene035678.t1	AT2G33500	44.944	89	49	0	22	110	12	100	1.78e-18	85.9

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Find 74 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTAAACCTACTCCAGCG+CGG	0.776053	8.1:-3943046	MS.gene035678:CDS
AAAGTGGGAACGACGCCGTA+TGG	0.475583	8.1:+3942471	None:intergenic
AACAACGAAATTGAAAGACG+CGG	0.720436	8.1:+3943286	None:intergenic
AACCGAAATTGACTCGGATG+TGG	0.481901	8.1:-3942553	MS.gene035678:CDS
AAGATGGGTATTGAAAGAGG+AGG	0.750089	8.1:-3943671	MS.gene035678:CDS
ACATCCAGACGCGCTCGTGG+CGG	0.606168	8.1:+3943498	None:intergenic
ACCGGCCTTTAATTCTAGGC+CGG	0.502804	8.1:+3942586	None:intergenic
ACGACGAGCGAGTGGATTGG+CGG	0.592706	8.1:+3943388	None:intergenic
AGACGCGGCTGCTGCAGCAG+CGG	0.489035	8.1:+3943301	None:intergenic
AGAGATGTTCTTCTCTGATA+TGG	0.330404	8.1:-3943156	MS.gene035678:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAGAAATGCAGAATCAGAA+CGG	+	chr8.1:3942571-3942590	None:intergenic	30.0%
ATCTTGAGTTTCATTGAGTT+TGG	+	chr8.1:3942948-3942967	None:intergenic	30.0%
ATGATGAATCATAACTCAGA+AGG	-	chr8.1:3943112-3943131	MS.gene035678:CDS	30.0%
AACAACGAAATTGAAAGACG+CGG	+	chr8.1:3942852-3942871	None:intergenic	35.0%
AGAGATGTTCTTCTCTGATA+TGG	-	chr8.1:3942979-3942998	MS.gene035678:intron	35.0%
AGCAACACAATTATGTGGAA+TGG	-	chr8.1:3943429-3943448	MS.gene035678:CDS	35.0%
AGTTCCTTCATCTATGCTAA+TGG	-	chr8.1:3943636-3943655	MS.gene035678:CDS	35.0%
ATTACTTACGCTATGGCTAA+TGG	+	chr8.1:3943197-3943216	None:intergenic	35.0%
CTGATTCCGAATCCTAATTT+CGG	-	chr8.1:3942920-3942939	MS.gene035678:intron	35.0%
TAAACGAACCGAAATTGACT+CGG	-	chr8.1:3943576-3943595	MS.gene035678:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	3942467	3943690	3942467	ID=MS.gene035678
chr8.1	mRNA	3942467	3943690	3942467	ID=MS.gene035678.t1;Parent=MS.gene035678
chr8.1	exon	3943024	3943690	3943024	ID=MS.gene035678.t1.exon1;Parent=MS.gene035678.t1
chr8.1	CDS	3943024	3943690	3943024	ID=cds.MS.gene035678.t1;Parent=MS.gene035678.t1
chr8.1	exon	3942467	3942762	3942467	ID=MS.gene035678.t1.exon2;Parent=MS.gene035678.t1
chr8.1	CDS	3942467	3942762	3942467	ID=cds.MS.gene035678.t1;Parent=MS.gene035678.t1

Gene Sequence

>MS.gene035678

ATGGGTATTGAAAGAGGAGGACTAAAGAGTTTTCGAAGCGGCTGGAGTGTGCCACCTAAGCTATGTGATTCTTGCAAGCTTACTCCCGCCGCGCTTTTTTGCCGTTCTGATTCTGCATTTCTATGCATCAACTGTGATTCCACCATTCACTCTGCTAACAAACTCTCTTCCCGCCACGAGCGCGTCTGGATGTGTGAAGTTTGTGAACAAGCACCAGCTTCCGTCACGTGCAAAGCTGACGCAGCTGCTCTATGCGTCACGTGCGATTCCGACATCCACTCCGCCAATCCACTCGCTCGTCGTCACGAACGTGTTCCCGTCGAGCCTTTCTTTGACTCCGCTGAATCCGTCGTCAAATCCTCCACCGCCGCTGCTGCAGCAGCCGCGTCTTTCAATTTCGTTGTTCCTACTGACGACGGTTACGGTCAAGATGATGCTGAAGCAGCTGCGTGGCTGATTCCGAATCCTAATTTCGGATCCAAACTCAATGAAACTCAAGATATCAAAACTAGAGAGATGTTCTTCTCTGATATGGATCCATTTCTTGATTTTGATTACTCAAACAATTTCCAGAACAATATTAACTGCAGCAACGCCATGAACGATAGCGTTGTTCCTGTTCAAACTAAACCTACTCCAGCGCGGATGATGAATCATAACTCAGAAGGTTGCTTTGACATTGATTTTTGCAGATCGAAGCTATCTTCCTTTAACTATCCATCACATTCCATTAGCCATAGCGTAAGTAATTAATTAATGATTTGATTAATTAAGTAATAAGTGTCATTAGAAGTTAATTAATTAATTAATAATGATTTGGTTTGTTATTAGCAGGTTTCGTCATCTTCACTTGACGTAGGAGTGGTGCCAGATGCAAACACGGTGTCAGAGATATCATACAATTTTGGATCGGAATCGATGGTTTCAGGGGGTGTGAATAGTAACCAAGGGGTGGTGCAAGGAGCAACACAATTATGTGGAATGGATCGTGAAGCGAGGGTTATGAGGTACAGAGAGAAGAGGAAGAACCGAAAATTCGAGAAGACTATTCGTTATGCTTCTCGAAAAGCTTATGCAGAAACCCGGCCTAGAATTAAAGGCCGGTTCGCTAAACGAACCGAAATTGACTCGGATGTGGACCGTCTCTATAACCCTGCTGATCCTCTCGCAGTTCCTTCATCTATGCTAATGGACTGTCCATACGGCGTCGTTCCCACTTTTTAG

Protein sequence

>MS.gene035678.t1

MGIERGGLKSFRSGWSVPPKLCDSCKLTPAALFCRSDSAFLCINCDSTIHSANKLSSRHERVWMCEVCEQAPASVTCKADAAALCVTCDSDIHSANPLARRHERVPVEPFFDSAESVVKSSTAAAAAAASFNFVVPTDDGYGQDDAEAAAWLIPNPNFGSKLNETQDIKTREMFFSDMDPFLDFDYSNNFQNNINCSNAMNDSVVPVQTKPTPARMMNHNSEGGVNSNQGVVQGATQLCGMDREARVMRYREKRKNRKFEKTIRYASRKAYAETRPRIKGRFAKRTEIDSDVDRLYNPADPLAVPSSMLMDCPYGVVPTF