AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene03694


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03694.t1 MTR_3g045440 97.026 269 8 0 1 269 1 269 0.0 543
MS.gene03694.t1 MTR_6g086560 63.498 263 95 1 8 269 17 279 2.31e-125 358
MS.gene03694.t1 MTR_1g018320 38.077 260 159 2 7 265 5 263 3.78e-73 225
MS.gene03694.t1 MTR_4g095310 40.816 245 143 2 13 256 9 252 1.42e-70 218
MS.gene03694.t1 MTR_5g016150 38.776 245 148 2 13 256 9 252 7.29e-68 213
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03694.t1 AT3G24420 50.185 271 132 2 1 268 1 271 3.00e-104 304
MS.gene03694.t1 AT3G03990 38.697 261 159 1 5 265 3 262 8.24e-76 231
MS.gene03694.t1 AT4G37470 42.041 245 140 2 13 256 9 252 1.99e-71 221

Find 53 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTAATGCCCTATGCTTAT+TGG 0.241237 3.4:+35899320 None:intergenic
TGACATAAAACTATTGTTTC+GGG 0.281340 3.4:+35900577 None:intergenic
TACTTGGATGAGAACATTAA+AGG 0.285814 3.4:-35899302 MS.gene03694:CDS
ATGACATAAAACTATTGTTT+CGG 0.322926 3.4:+35900576 None:intergenic
TTGGAACAGACCAGTCTATA+TGG 0.354404 3.4:-35900549 MS.gene03694:CDS
TCAATAGAGTCAGCATCATT+TGG 0.360021 3.4:+35899488 None:intergenic
ACAGTCTAGTTCTGTTTGAT+TGG 0.364702 3.4:-35900495 MS.gene03694:CDS
TGATGAGTTAATCACTCTTA+TGG 0.373825 3.4:-35900401 MS.gene03694:CDS
TGAGGTGCTGAAGGGTATTG+TGG 0.383521 3.4:-35899210 MS.gene03694:CDS
AATAAGCATAGGGCATTACT+TGG 0.397845 3.4:-35899318 MS.gene03694:CDS
GCTTTCGTATTGAGACTCTA+TGG 0.399369 3.4:+35899544 None:intergenic
TCTGTAATAGCTCCAGAAAA+TGG 0.417553 3.4:+35900472 None:intergenic
TCATTTGGATCCACTGCAAT+AGG 0.424577 3.4:+35899503 None:intergenic
AAAAGCCTGAGCTCTTTAAG+AGG 0.425563 3.4:-35900303 MS.gene03694:CDS
TAGAGTCTCAATACGAAAGC+TGG 0.436550 3.4:-35899541 MS.gene03694:CDS
CTCATCAGCATAAGGTTCAT+AGG 0.436848 3.4:+35900417 None:intergenic
ACTTGGATGAGAACATTAAA+GGG 0.437938 3.4:-35899301 MS.gene03694:CDS
GAGGTGCTGAAGGGTATTGT+GGG 0.441840 3.4:-35899209 MS.gene03694:CDS
CTATAGAGATATAGTTGAAA+AGG 0.445888 3.4:-35899390 MS.gene03694:CDS
CATTAAAGGGATGTCTACTT+TGG 0.446680 3.4:-35899288 MS.gene03694:CDS
ATAAATACAGATGACTATAA+AGG 0.458026 3.4:-35899605 MS.gene03694:CDS
ACAATCCTCTTAAAGAGCTC+AGG 0.466269 3.4:+35900298 None:intergenic
TGTTTCGGGTCCTGAGCCTA+TGG 0.470244 3.4:+35900591 None:intergenic
CACTCTATGTCTGCTATGAT+AGG 0.473029 3.4:-35900343 MS.gene03694:CDS
TTCTTCTTTGTGCTTCCCCA+AGG 0.483467 3.4:-35900276 MS.gene03694:intron
GTGATTAACTCATCAGCATA+AGG 0.495421 3.4:+35900409 None:intergenic
AATCACTCTTATGGATGAGT+TGG 0.497837 3.4:-35900392 MS.gene03694:CDS
CAACAAAAGTGATACATTTG+AGG 0.498332 3.4:+35900368 None:intergenic
CTCAGTTAACTGCACACCTA+AGG 0.500170 3.4:-35899238 MS.gene03694:CDS
GTGCAGTTAACTGAGGGAAA+TGG 0.509673 3.4:+35899246 None:intergenic
TCAGCACCTCAACCAACCTT+AGG 0.510018 3.4:+35899222 None:intergenic
TTGGAGTTTATAGATATGAC+TGG 0.515619 3.4:-35899269 MS.gene03694:CDS
AAGGTTGGTTGAGGTGCTGA+AGG 0.529671 3.4:-35899219 MS.gene03694:CDS
AATGCAAGAACCATAGGCTC+AGG 0.553052 3.4:-35900601 MS.gene03694:CDS
GAAAGCTGGGTTTCAGCATA+CGG 0.556562 3.4:-35899527 MS.gene03694:CDS
TCTTTAAATGCAAGAACCAT+AGG 0.556752 3.4:-35900607 MS.gene03694:CDS
GTGGCAGTTCCAATAAGCAT+AGG 0.560917 3.4:-35899329 MS.gene03694:CDS
ACTTGACTGAATGATTGTGC+AGG 0.563263 3.4:+35899358 None:intergenic
AGCATACGGTCCTATTGCAG+TGG 0.564652 3.4:-35899513 MS.gene03694:CDS
TTGCATTTAAAGATGTTGAG+AGG 0.576785 3.4:+35900617 None:intergenic
AGAGTCTCAATACGAAAGCT+GGG 0.578249 3.4:-35899540 MS.gene03694:CDS
TGCACACCTAAGGTTGGTTG+AGG 0.583937 3.4:-35899228 MS.gene03694:CDS
TGGCAGTTCCAATAAGCATA+GGG 0.586920 3.4:-35899328 MS.gene03694:CDS
GTTAACTGCACACCTAAGGT+TGG 0.590880 3.4:-35899234 MS.gene03694:CDS
CTTGACTGAATGATTGTGCA+GGG 0.604202 3.4:+35899359 None:intergenic
AATACAGATGACTATAAAGG+AGG 0.635005 3.4:-35899602 MS.gene03694:CDS
CATTCAGTCAAGTAATGATG+TGG 0.635222 3.4:-35899348 MS.gene03694:CDS
TGGTGAAGTTGCAATTACCT+TGG 0.640152 3.4:-35899435 MS.gene03694:CDS
CTTAGGTGTGCAGTTAACTG+AGG 0.642434 3.4:+35899239 None:intergenic
AGGTTGGTTGAGGTGCTGAA+GGG 0.645453 3.4:-35899218 MS.gene03694:CDS
TTAGGTGTGCAGTTAACTGA+GGG 0.650507 3.4:+35899240 None:intergenic
AAATGCTTGAAAAGAATGGG+TGG 0.686067 3.4:-35899455 MS.gene03694:CDS
TTGACTGAATGATTGTGCAG+GGG 0.705699 3.4:+35899360 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAACTTTTTAACTATGTTAT+TGG - chr3.4:35899942-35899961 MS.gene03694:intron 15.0%
!!! TATATTTTGCATGATTATAA+AGG + chr3.4:35899402-35899421 None:intergenic 15.0%
!! AATTAGTTATTGACAAAACT+CGG + chr3.4:35899981-35900000 None:intergenic 20.0%
!! ACCTATGAATGAATAATTAT+GGG + chr3.4:35900215-35900234 None:intergenic 20.0%
!! ATAAATACAGATGACTATAA+AGG - chr3.4:35900237-35900256 MS.gene03694:intron 20.0%
!! ATGACATAAAACTATTGTTT+CGG + chr3.4:35899269-35899288 None:intergenic 20.0%
!! TACCTATGAATGAATAATTA+TGG + chr3.4:35900216-35900235 None:intergenic 20.0%
!!! AAACAATAGTTTTATGTCAT+GGG - chr3.4:35899269-35899288 MS.gene03694:CDS 20.0%
! ATTAGTTATTGACAAAACTC+GGG + chr3.4:35899980-35899999 None:intergenic 25.0%
! CAAAAATGCTTGAAAAGAAT+GGG - chr3.4:35900384-35900403 MS.gene03694:CDS 25.0%
! CTATAGAGATATAGTTGAAA+AGG - chr3.4:35900452-35900471 MS.gene03694:CDS 25.0%
! GAAATCTCATATTGTGTATT+TGG - chr3.4:35899715-35899734 MS.gene03694:intron 25.0%
! TCAAAAATGCTTGAAAAGAA+TGG - chr3.4:35900383-35900402 MS.gene03694:CDS 25.0%
! TCCCATAATTATTCATTCAT+AGG - chr3.4:35900211-35900230 MS.gene03694:intron 25.0%
! TGACATAAAACTATTGTTTC+GGG + chr3.4:35899268-35899287 None:intergenic 25.0%
! TGTTAAAACGCTTGATTATT+TGG - chr3.4:35899691-35899710 MS.gene03694:intron 25.0%
!! AAACATTTGATGGTACAATA+TGG - chr3.4:35900161-35900180 MS.gene03694:intron 25.0%
!! AACATTTGATGGTACAATAT+GGG - chr3.4:35900162-35900181 MS.gene03694:intron 25.0%
!! CAATTTTATTCCATATAGAC+TGG + chr3.4:35899306-35899325 None:intergenic 25.0%
!! CTGTAATTTTCAGTTAAAAG+AGG + chr3.4:35899331-35899350 None:intergenic 25.0%
!!! GAAACAATAGTTTTATGTCA+TGG - chr3.4:35899268-35899287 MS.gene03694:CDS 25.0%
AATACAGATGACTATAAAGG+AGG - chr3.4:35900240-35900259 MS.gene03694:intron 30.0%
ACTTGGATGAGAACATTAAA+GGG - chr3.4:35900541-35900560 MS.gene03694:CDS 30.0%
CAACAAAAGTGATACATTTG+AGG + chr3.4:35899477-35899496 None:intergenic 30.0%
GTACCATCAAATGTTTGAAT+TGG + chr3.4:35900157-35900176 None:intergenic 30.0%
TACTTGGATGAGAACATTAA+AGG - chr3.4:35900540-35900559 MS.gene03694:CDS 30.0%
TCTTTAAATGCAAGAACCAT+AGG - chr3.4:35899235-35899254 MS.gene03694:CDS 30.0%
TGATGAGTTAATCACTCTTA+TGG - chr3.4:35899441-35899460 MS.gene03694:CDS 30.0%
TTAGTTATTGACAAAACTCG+GGG + chr3.4:35899979-35899998 None:intergenic 30.0%
TTGCATTTAAAGATGTTGAG+AGG + chr3.4:35899228-35899247 None:intergenic 30.0%
TTGGAGTTTATAGATATGAC+TGG - chr3.4:35900573-35900592 MS.gene03694:CDS 30.0%
! TTTTGTGGAAGGACAAAAAT+AGG + chr3.4:35900088-35900107 None:intergenic 30.0%
!! CTCAAATGTATCACTTTTGT+TGG - chr3.4:35899475-35899494 MS.gene03694:CDS 30.0%
!!! ATAGTTTTATGTCATGGGTT+TGG - chr3.4:35899274-35899293 MS.gene03694:CDS 30.0%
!!! GTTTAGCATTTCTAGTTTTG+TGG + chr3.4:35900103-35900122 None:intergenic 30.0%
AAATGCTTGAAAAGAATGGG+TGG - chr3.4:35900387-35900406 MS.gene03694:CDS 35.0%
AAGTAATGCCCTATGCTTAT+TGG + chr3.4:35900525-35900544 None:intergenic 35.0%
AATCACTCTTATGGATGAGT+TGG - chr3.4:35899450-35899469 MS.gene03694:CDS 35.0%
AGGCCAATTCAAACATTTGA+TGG - chr3.4:35900151-35900170 MS.gene03694:intron 35.0%
CATTAAAGGGATGTCTACTT+TGG - chr3.4:35900554-35900573 MS.gene03694:CDS 35.0%
CATTCAGTCAAGTAATGATG+TGG - chr3.4:35900494-35900513 MS.gene03694:CDS 35.0%
GAAAAACATAGATGTGAGAG+AGG + chr3.4:35900040-35900059 None:intergenic 35.0%
GTGATTAACTCATCAGCATA+AGG + chr3.4:35899436-35899455 None:intergenic 35.0%
TAGTTATTGACAAAACTCGG+GGG + chr3.4:35899978-35899997 None:intergenic 35.0%
TCAATAGAGTCAGCATCATT+TGG + chr3.4:35900357-35900376 None:intergenic 35.0%
TCTGTAATAGCTCCAGAAAA+TGG + chr3.4:35899373-35899392 None:intergenic 35.0%
! AAAGAGCTCAGGCTTTTTAA+TGG + chr3.4:35899536-35899555 None:intergenic 35.0%
! AATAAGCATAGGGCATTACT+TGG - chr3.4:35900524-35900543 MS.gene03694:CDS 35.0%
! ACATCAGAGAGAGTATATGT+AGG + chr3.4:35899606-35899625 None:intergenic 35.0%
! ATTTGATGGTACAATATGGG+TGG - chr3.4:35900165-35900184 MS.gene03694:intron 35.0%
! GTCACTGAAAAACACAGTTT+TGG + chr3.4:35900433-35900452 None:intergenic 35.0%
! TCAAATGTTTGAATTGGCCT+AGG + chr3.4:35900151-35900170 None:intergenic 35.0%
!! ACAGTCTAGTTCTGTTTGAT+TGG - chr3.4:35899347-35899366 MS.gene03694:CDS 35.0%
!!! AGCATTTCTAGTTTTGTGGA+AGG + chr3.4:35900099-35900118 None:intergenic 35.0%
!!! TCACTTTTTTTTCCTAGCCT+AGG - chr3.4:35900131-35900150 MS.gene03694:intron 35.0%
AAAAGCCTGAGCTCTTTAAG+AGG - chr3.4:35899539-35899558 MS.gene03694:CDS 40.0%
ACAATCCTCTTAAAGAGCTC+AGG + chr3.4:35899547-35899566 None:intergenic 40.0%
ACTTGACTGAATGATTGTGC+AGG + chr3.4:35900487-35900506 None:intergenic 40.0%
AGAGTCTCAATACGAAAGCT+GGG - chr3.4:35900302-35900321 MS.gene03694:CDS 40.0%
CACTCTATGTCTGCTATGAT+AGG - chr3.4:35899499-35899518 MS.gene03694:CDS 40.0%
CATGTGTTACCGACATATGT+CGG - chr3.4:35899846-35899865 MS.gene03694:intron 40.0%
CTCATCAGCATAAGGTTCAT+AGG + chr3.4:35899428-35899447 None:intergenic 40.0%
CTTGACTGAATGATTGTGCA+GGG + chr3.4:35900486-35900505 None:intergenic 40.0%
GCTTTCGTATTGAGACTCTA+TGG + chr3.4:35900301-35900320 None:intergenic 40.0%
GTGCACTTCTTTATGTGTTG+TGG + chr3.4:35900066-35900085 None:intergenic 40.0%
TAGAGTCTCAATACGAAAGC+TGG - chr3.4:35900301-35900320 MS.gene03694:CDS 40.0%
TAGTGTCAACGTGTTAGTGA+CGG + chr3.4:35899783-35899802 None:intergenic 40.0%
TCATTTGGATCCACTGCAAT+AGG + chr3.4:35900342-35900361 None:intergenic 40.0%
TGGCAGTTCCAATAAGCATA+GGG - chr3.4:35900514-35900533 MS.gene03694:CDS 40.0%
TGGTGAAGTTGCAATTACCT+TGG - chr3.4:35900407-35900426 MS.gene03694:CDS 40.0%
TTAGGTGTGCAGTTAACTGA+GGG + chr3.4:35900605-35900624 None:intergenic 40.0%
TTGACTGAATGATTGTGCAG+GGG + chr3.4:35900485-35900504 None:intergenic 40.0%
TTGGAACAGACCAGTCTATA+TGG - chr3.4:35899293-35899312 MS.gene03694:CDS 40.0%
! GAGAGAGGCTACTTTAAAGA+TGG + chr3.4:35900025-35900044 None:intergenic 40.0%
!! AGGTAAAGATGAGAGTACCT+TGG + chr3.4:35899586-35899605 None:intergenic 40.0%
!! CTGTTTGATTGGCCATTTTC+TGG - chr3.4:35899358-35899377 MS.gene03694:CDS 40.0%
!! GAAAAACACAGTTTTGGCCA+AGG + chr3.4:35900427-35900446 None:intergenic 40.0%
!! GGTAAAGATGAGAGTACCTT+GGG + chr3.4:35899585-35899604 None:intergenic 40.0%
!! GTAAAGATGAGAGTACCTTG+GGG + chr3.4:35899584-35899603 None:intergenic 40.0%
!!! GATGTTGAGAGGCTCTTTTT+TGG + chr3.4:35899217-35899236 None:intergenic 40.0%
AATGCAAGAACCATAGGCTC+AGG - chr3.4:35899241-35899260 MS.gene03694:CDS 45.0%
CTCAGTTAACTGCACACCTA+AGG - chr3.4:35900604-35900623 MS.gene03694:CDS 45.0%
CTTAGGTGTGCAGTTAACTG+AGG + chr3.4:35900606-35900625 None:intergenic 45.0%
GAAAGCTGGGTTTCAGCATA+CGG - chr3.4:35900315-35900334 MS.gene03694:CDS 45.0%
GTCTGATGTCCGACATATGT+CGG + chr3.4:35899858-35899877 None:intergenic 45.0%
GTGCAGTTAACTGAGGGAAA+TGG + chr3.4:35900599-35900618 None:intergenic 45.0%
GTGGCAGTTCCAATAAGCAT+AGG - chr3.4:35900513-35900532 MS.gene03694:CDS 45.0%
GTTAACTGCACACCTAAGGT+TGG - chr3.4:35900608-35900627 MS.gene03694:CDS 45.0%
TTCTTCTTTGTGCTTCCCCA+AGG - chr3.4:35899566-35899585 MS.gene03694:CDS 45.0%
! ACGCTTTCCATCAGAAGTGT+CGG - chr3.4:35899878-35899897 MS.gene03694:intron 45.0%
! GAGCTCAGGCTTTTTAATGG+AGG + chr3.4:35899533-35899552 None:intergenic 45.0%
! TGTTTGAATTGGCCTAGGCT+AGG + chr3.4:35900146-35900165 None:intergenic 45.0%
!! GTGTCGGTGCTACAAAGATT+TGG - chr3.4:35899894-35899913 MS.gene03694:intron 45.0%
AGCATACGGTCCTATTGCAG+TGG - chr3.4:35900329-35900348 MS.gene03694:CDS 50.0%
TCAGCACCTCAACCAACCTT+AGG + chr3.4:35900623-35900642 None:intergenic 50.0%
! TGCACACCTAAGGTTGGTTG+AGG - chr3.4:35900614-35900633 MS.gene03694:CDS 50.0%
!! AAGGTTGGTTGAGGTGCTGA+AGG - chr3.4:35900623-35900642 MS.gene03694:CDS 50.0%
!! AGGTTGGTTGAGGTGCTGAA+GGG - chr3.4:35900624-35900643 MS.gene03694:CDS 50.0%
!! GAGGTGCTGAAGGGTATTGT+GGG - chr3.4:35900633-35900652 MS.gene03694:CDS 50.0%
!! TGAGGTGCTGAAGGGTATTG+TGG - chr3.4:35900632-35900651 MS.gene03694:CDS 50.0%
!! TGTAGCACCGACACTTCTGA+TGG + chr3.4:35899888-35899907 None:intergenic 50.0%
! TGTTTCGGGTCCTGAGCCTA+TGG + chr3.4:35899254-35899273 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 35899205 35900659 35899205 ID=MS.gene03694
chr3.4 mRNA 35899205 35900659 35899205 ID=MS.gene03694.t1;Parent=MS.gene03694
chr3.4 exon 35900277 35900659 35900277 ID=MS.gene03694.t1.exon1;Parent=MS.gene03694.t1
chr3.4 CDS 35900277 35900659 35900277 ID=cds.MS.gene03694.t1;Parent=MS.gene03694.t1
chr3.4 exon 35899205 35899631 35899205 ID=MS.gene03694.t1.exon2;Parent=MS.gene03694.t1
chr3.4 CDS 35899205 35899631 35899205 ID=cds.MS.gene03694.t1;Parent=MS.gene03694.t1
Gene Sequence

>MS.gene03694

ATGGTGACACCAAAAAAGAGCCTCTCAACATCTTTAAATGCAAGAACCATAGGCTCAGGACCCGAAACAATAGTTTTATGTCATGGGTTTGGAACAGACCAGTCTATATGGAATAAAATTGTTCCTCTTTTAACTGAAAATTACAGTCTAGTTCTGTTTGATTGGCCATTTTCTGGAGCTATTACAGATAAAAGCCTTTATAATCATGCAAAATATAATTCCTATGAACCTTATGCTGATGAGTTAATCACTCTTATGGATGAGTTGGACCTCAAATGTATCACTTTTGTTGGACACTCTATGTCTGCTATGATAGGTTGCATTGCCTCCATTAAAAAGCCTGAGCTCTTTAAGAGGATTGTTCTTCTTTGTGCTTCCCCAAGGTACTCTCATCTTTACCTACATATACTCTCTCTGATGTTGTCTTTTTTTTTTTATTCTCTTTTTATGAAAATACTAGAAGTAACACAATATTTGAGATTCTGTTGTTAAAACGCTTGATTATTTGGTGAAATCTCATATTGTGTATTTGGATCTATGAAGTATCGTCACAAACACTAATATCATATAAGACACCGTCACTAACACGTTGACACTAGTAAAAATATGAAGAAAAAAAATGAATTAATTGAACGTAATCACATGTGTTACCGACATATGTCGGACATCAGACACGCTTTCCATCAGAAGTGTCGGTGCTACAAAGATTTGGATAAATATTTGAAAGTAATTTAAAGTAACTTTTTAACTATGTTATTGGTGCAAAGTCTCCCCCGAGTTTTGTCAATAACTAATTTCTGTCGAGAAAATTCACTAACCATCTTTAAAGTAGCCTCTCTCACATCTATGTTTTTCTATCCACAACACATAAAGAAGTGCACCTATTTTTGTCCTTCCACAAAACTAGAAATGCTAAACTCGATAAATCACTTTTTTTTCCTAGCCTAGGCCAATTCAAACATTTGATGGTACAATATGGGTGGAATCAAATAGTCTTGCATTAACATCCCATAATTATTCATTCATAGGTATATAAATACAGATGACTATAAAGGAGGCTTTGAGAGATCAGATATTGAGAACTTAATATCAACCATAGAGTCTCAATACGAAAGCTGGGTTTCAGCATACGGTCCTATTGCAGTGGATCCAAATGATGCTGACTCTATTGACAAATTTCAAAAATGCTTGAAAAGAATGGGTGGTGAAGTTGCAATTACCTTGGCCAAAACTGTGTTTTTCAGTGACTATAGAGATATAGTTGAAAAGGTAGTGATCCCCTGCACAATCATTCAGTCAAGTAATGATGTGGCAGTTCCAATAAGCATAGGGCATTACTTGGATGAGAACATTAAAGGGATGTCTACTTTGGAGTTTATAGATATGACTGGCCATTTCCCTCAGTTAACTGCACACCTAAGGTTGGTTGAGGTGCTGAAGGGTATTGTGGGTTAG

Protein sequence

>MS.gene03694.t1

MVTPKKSLSTSLNARTIGSGPETIVLCHGFGTDQSIWNKIVPLLTENYSLVLFDWPFSGAITDKSLYNHAKYNSYEPYADELITLMDELDLKCITFVGHSMSAMIGCIASIKKPELFKRIVLLCASPRYINTDDYKGGFERSDIENLISTIESQYESWVSAYGPIAVDPNDADSIDKFQKCLKRMGGEVAITLAKTVFFSDYRDIVEKVVIPCTIIQSSNDVAVPISIGHYLDENIKGMSTLEFIDMTGHFPQLTAHLRLVEVLKGIVG