Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037374.t1 | XP_004497247.1 | 84 | 219 | 35 | 0 | 1 | 219 | 1 | 219 | 1.10E-99 | 372.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037374.t1 | Q9FMW4 | 58.4 | 202 | 84 | 0 | 16 | 217 | 18 | 219 | 4.1e-60 | 232.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037374.t1 | A0A1S2Y1F9 | 84.0 | 219 | 35 | 0 | 1 | 219 | 1 | 219 | 7.6e-100 | 372.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene93120 | MS.gene037374 | PPI |
| MS.gene93121 | MS.gene037374 | PPI |
| MS.gene040857 | MS.gene037374 | PPI |
| MS.gene93119 | MS.gene037374 | PPI |
| MS.gene037375 | MS.gene037374 | PPI |
| MS.gene061035 | MS.gene037374 | PPI |
| MS.gene026428 | MS.gene037374 | PPI |
| MS.gene037373 | MS.gene037374 | PPI |
| MS.gene061654 | MS.gene037374 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037374.t1 | MTR_1g114640 | 83.486 | 218 | 29 | 1 | 1 | 211 | 1 | 218 | 2.24e-132 | 371 |
| MS.gene037374.t1 | MTR_1g033750 | 64.091 | 220 | 69 | 2 | 5 | 214 | 4 | 223 | 2.15e-97 | 283 |
| MS.gene037374.t1 | MTR_1g114640 | 81.818 | 165 | 23 | 1 | 1 | 158 | 1 | 165 | 6.35e-93 | 270 |
| MS.gene037374.t1 | MTR_1g114690 | 54.386 | 228 | 80 | 3 | 1 | 214 | 1 | 218 | 2.95e-79 | 236 |
| MS.gene037374.t1 | MTR_1g114690 | 53.304 | 227 | 82 | 3 | 1 | 213 | 1 | 217 | 3.61e-77 | 231 |
| MS.gene037374.t1 | MTR_1g114690 | 47.710 | 262 | 79 | 4 | 1 | 214 | 1 | 252 | 6.61e-72 | 219 |
| MS.gene037374.t1 | MTR_1g114750 | 49.107 | 224 | 101 | 3 | 1 | 214 | 1 | 221 | 4.24e-71 | 216 |
| MS.gene037374.t1 | MTR_1g114680 | 93.103 | 116 | 8 | 0 | 104 | 219 | 53 | 168 | 3.63e-68 | 207 |
| MS.gene037374.t1 | MTR_8g100065 | 58.896 | 163 | 66 | 1 | 53 | 214 | 23 | 185 | 2.14e-63 | 195 |
| MS.gene037374.t1 | MTR_6g077990 | 55.625 | 160 | 71 | 0 | 57 | 216 | 41 | 200 | 7.41e-63 | 194 |
| MS.gene037374.t1 | MTR_6g077990 | 55.625 | 160 | 71 | 0 | 57 | 216 | 42 | 201 | 7.57e-63 | 194 |
| MS.gene037374.t1 | MTR_8g100065 | 58.896 | 163 | 66 | 1 | 53 | 214 | 45 | 207 | 9.87e-63 | 194 |
| MS.gene037374.t1 | MTR_1g115130 | 59.333 | 150 | 61 | 0 | 66 | 215 | 46 | 195 | 1.14e-61 | 191 |
| MS.gene037374.t1 | MTR_6g077990 | 55.629 | 151 | 67 | 0 | 66 | 216 | 8 | 158 | 1.42e-58 | 182 |
| MS.gene037374.t1 | MTR_1g114690 | 52.121 | 165 | 71 | 2 | 1 | 159 | 1 | 163 | 1.28e-49 | 160 |
| MS.gene037374.t1 | MTR_7g083570 | 35.417 | 192 | 124 | 0 | 21 | 212 | 84 | 275 | 9.47e-43 | 145 |
| MS.gene037374.t1 | MTR_8g069110 | 35.912 | 181 | 105 | 2 | 41 | 211 | 88 | 267 | 6.76e-42 | 143 |
| MS.gene037374.t1 | MTR_3g100750 | 32.850 | 207 | 127 | 3 | 16 | 211 | 50 | 255 | 5.60e-41 | 140 |
| MS.gene037374.t1 | MTR_8g100065 | 59.813 | 107 | 42 | 1 | 53 | 158 | 23 | 129 | 1.22e-38 | 130 |
| MS.gene037374.t1 | MTR_6g077990 | 52.137 | 117 | 56 | 0 | 42 | 158 | 26 | 142 | 1.39e-38 | 131 |
| MS.gene037374.t1 | MTR_8g100065 | 59.813 | 107 | 42 | 1 | 53 | 158 | 45 | 151 | 4.05e-38 | 130 |
| MS.gene037374.t1 | MTR_5g015750 | 79.167 | 72 | 8 | 1 | 1 | 65 | 1 | 72 | 4.82e-33 | 115 |
| MS.gene037374.t1 | MTR_2g076350 | 33.854 | 192 | 126 | 1 | 23 | 213 | 36 | 227 | 6.32e-33 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene037374.t1 | AT5G23370 | 56.881 | 218 | 88 | 2 | 1 | 214 | 1 | 216 | 1.84e-81 | 242 |
| MS.gene037374.t1 | AT5G23350 | 52.889 | 225 | 90 | 4 | 1 | 216 | 1 | 218 | 1.68e-77 | 232 |
| MS.gene037374.t1 | AT5G08350 | 57.071 | 198 | 76 | 2 | 21 | 214 | 23 | 215 | 8.02e-77 | 231 |
| MS.gene037374.t1 | AT5G23360 | 53.960 | 202 | 83 | 2 | 16 | 216 | 18 | 210 | 4.14e-75 | 226 |
| MS.gene037374.t1 | AT4G01600 | 40.341 | 176 | 104 | 1 | 41 | 215 | 14 | 189 | 1.82e-47 | 155 |
| MS.gene037374.t1 | AT4G01600 | 40.341 | 176 | 104 | 1 | 41 | 215 | 53 | 228 | 5.30e-47 | 155 |
| MS.gene037374.t1 | AT5G13200 | 36.170 | 188 | 120 | 0 | 28 | 215 | 77 | 264 | 6.29e-44 | 148 |
| MS.gene037374.t1 | AT4G01600 | 43.046 | 151 | 85 | 1 | 66 | 215 | 73 | 223 | 2.08e-42 | 143 |
| MS.gene037374.t1 | AT1G28200 | 35.354 | 198 | 117 | 4 | 22 | 211 | 60 | 254 | 1.59e-41 | 142 |
| MS.gene037374.t1 | AT1G28200 | 35.354 | 198 | 117 | 4 | 22 | 211 | 60 | 254 | 1.59e-41 | 142 |
| MS.gene037374.t1 | AT2G22475 | 38.509 | 161 | 96 | 2 | 52 | 211 | 133 | 291 | 4.34e-41 | 142 |
| MS.gene037374.t1 | AT5G13200 | 33.333 | 132 | 88 | 0 | 28 | 159 | 77 | 208 | 2.53e-25 | 99.0 |
| MS.gene037374.t1 | AT4G40100 | 28.177 | 181 | 95 | 2 | 31 | 211 | 71 | 216 | 6.30e-22 | 90.1 |
| MS.gene037374.t1 | AT2G22475 | 34.746 | 118 | 74 | 2 | 52 | 168 | 133 | 248 | 2.23e-20 | 86.7 |
Find 39 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTTCATGAGTTACTTAA+TGG | 0.146739 | 1.3:-77687150 | MS.gene037374:CDS |
| AAGATAACAGATACAGTAAA+AGG | 0.228633 | 1.3:-77686710 | MS.gene037374:CDS |
| TTTACTGTATCTGTTATCTT+TGG | 0.247894 | 1.3:+77686713 | None:intergenic |
| AGATATCTGCCTGATTCTTC+TGG | 0.324067 | 1.3:-77687105 | MS.gene037374:CDS |
| GTTGGGAGCCAGAATTCTTC+AGG | 0.336328 | 1.3:-77686678 | MS.gene037374:CDS |
| CCCGCCAACCTGAAGAATTC+TGG | 0.351542 | 1.3:+77686670 | None:intergenic |
| GATGAACATGTTTGGAAGAA+AGG | 0.373892 | 1.3:-77686931 | MS.gene037374:CDS |
| ATTCATTAGAGTTCACTATA+AGG | 0.401080 | 1.3:-77686471 | MS.gene037374:CDS |
| CTGTATATGCCAGAAGAATC+AGG | 0.412229 | 1.3:+77687096 | None:intergenic |
| ATGACTAAGATGAACATGTT+TGG | 0.423883 | 1.3:-77686939 | MS.gene037374:CDS |
| ATGAACATGTTTGGAAGAAA+GGG | 0.430526 | 1.3:-77686930 | MS.gene037374:CDS |
| CCAGAATTCTTCAGGTTGGC+GGG | 0.441065 | 1.3:-77686670 | MS.gene037374:CDS |
| GCCAGAATTCTTCAGGTTGG+CGG | 0.449134 | 1.3:-77686671 | MS.gene037374:CDS |
| CTCTAAATCTCAGAGGGAAA+AGG | 0.451562 | 1.3:-77686288 | MS.gene037374:CDS |
| ATCAAAATTTCTTCCTCGAA+TGG | 0.453468 | 1.3:-77686497 | MS.gene037374:CDS |
| ACCTTGCTTTGACTTTGTGA+TGG | 0.474385 | 1.3:+77687068 | None:intergenic |
| TCTAATGAATTCTCCATTCG+AGG | 0.480167 | 1.3:+77686484 | None:intergenic |
| ATGAAGAGAAGGCCAGCTAT+AGG | 0.493362 | 1.3:+77686557 | None:intergenic |
| AGTAAAAGGGAAACTGAGGT+TGG | 0.524536 | 1.3:-77686696 | MS.gene037374:CDS |
| GAGATTTAGAGATAGCCTGC+TGG | 0.531131 | 1.3:+77686300 | None:intergenic |
| ACCACGTCAGGTCCTATAGC+TGG | 0.538413 | 1.3:-77686569 | MS.gene037374:CDS |
| AGATAACAGATACAGTAAAA+GGG | 0.540258 | 1.3:-77686709 | MS.gene037374:CDS |
| GGAGCCAGAATTCTTCAGGT+TGG | 0.551383 | 1.3:-77686674 | MS.gene037374:CDS |
| TTGTAACCTGCAGTGAGACT+TGG | 0.558391 | 1.3:-77686737 | MS.gene037374:intron |
| AGCTTTAAGATATCTCCAGC+AGG | 0.564241 | 1.3:-77686315 | MS.gene037374:CDS |
| GGCTATCTCTAAATCTCAGA+GGG | 0.564713 | 1.3:-77686294 | MS.gene037374:CDS |
| GCACATGGAGTCCGAGAGCA+TGG | 0.566367 | 1.3:-77686897 | MS.gene037374:intron |
| GTAAAAGGGAAACTGAGGTT+GGG | 0.569074 | 1.3:-77686695 | MS.gene037374:CDS |
| TGTTACTTATCAACCACGTC+AGG | 0.572611 | 1.3:-77686581 | MS.gene037374:CDS |
| TCCATCACAAAGTCAAAGCA+AGG | 0.594218 | 1.3:-77687069 | MS.gene037374:intron |
| AAGATTACATACCATGCTCT+CGG | 0.595383 | 1.3:+77686886 | None:intergenic |
| ACAGAAAGCAACTTTGTGAG+TGG | 0.605272 | 1.3:+77686532 | None:intergenic |
| TGTGAGTGGAAATGAAGAGA+AGG | 0.611200 | 1.3:+77686546 | None:intergenic |
| CAGAATTCTTCAGGTTGGCG+GGG | 0.646633 | 1.3:-77686669 | MS.gene037374:CDS |
| AGGCAGATATCTCTTTGATG+AGG | 0.651918 | 1.3:+77687116 | None:intergenic |
| ATACAGTAAAAGGGAAACTG+AGG | 0.664591 | 1.3:-77686700 | MS.gene037374:CDS |
| AGGCTATCTCTAAATCTCAG+AGG | 0.678214 | 1.3:-77686295 | MS.gene037374:CDS |
| GCCAGCTATAGGACCTGACG+TGG | 0.697419 | 1.3:+77686568 | None:intergenic |
| ATACATAGAAATAGTCACTG+TGG | 0.703928 | 1.3:-77686381 | MS.gene037374:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAAATAATAAAGTTTTGTT+AGG | + | chr1.3:77686412-77686431 | None:intergenic | 15.0% |
| !!! | ACTTGTTGTATTTTTTCATA+TGG | + | chr1.3:77687008-77687027 | None:intergenic | 20.0% |
| !!! | ATTTCTATGTATTTTTCTGA+TGG | + | chr1.3:77687056-77687075 | None:intergenic | 20.0% |
| !!! | TTTAATGTCATTGATGATAA+TGG | - | chr1.3:77686449-77686468 | MS.gene037374:CDS | 20.0% |
| ! | AAGATAACAGATACAGTAAA+AGG | - | chr1.3:77686735-77686754 | MS.gene037374:intron | 25.0% |
| ! | AGATAACAGATACAGTAAAA+GGG | - | chr1.3:77686736-77686755 | MS.gene037374:intron | 25.0% |
| ! | ATTCATTAGAGTTCACTATA+AGG | - | chr1.3:77686974-77686993 | MS.gene037374:intron | 25.0% |
| ! | TAGAATGAAGTAGTAATTGA+TGG | - | chr1.3:77686672-77686691 | MS.gene037374:CDS | 25.0% |
| ! | TTTACTGTATCTGTTATCTT+TGG | + | chr1.3:77686735-77686754 | None:intergenic | 25.0% |
| ! | TTTCTTCATGAGTTACTTAA+TGG | - | chr1.3:77686295-77686314 | MS.gene037374:CDS | 25.0% |
| !! | AATGGTCATTTCATATTTTC+AGG | - | chr1.3:77686467-77686486 | MS.gene037374:CDS | 25.0% |
| !! | ATGATGCATCATACATATTT+TGG | + | chr1.3:77686628-77686647 | None:intergenic | 25.0% |
| !! | ATGATGCATCATTTGAAATT+TGG | - | chr1.3:77686636-77686655 | MS.gene037374:CDS | 25.0% |
| !! | CATTTGAAATTTGGAATGAT+TGG | - | chr1.3:77686645-77686664 | MS.gene037374:CDS | 25.0% |
| !!! | GTTTCATCATATTTTGACTT+TGG | + | chr1.3:77687034-77687053 | None:intergenic | 25.0% |
| ATACATAGAAATAGTCACTG+TGG | - | chr1.3:77687064-77687083 | MS.gene037374:intron | 30.0% | |
| ATCAAAATTTCTTCCTCGAA+TGG | - | chr1.3:77686948-77686967 | MS.gene037374:CDS | 30.0% | |
| ATGAACATGTTTGGAAGAAA+GGG | - | chr1.3:77686515-77686534 | MS.gene037374:CDS | 30.0% | |
| ATGACTAAGATGAACATGTT+TGG | - | chr1.3:77686506-77686525 | MS.gene037374:CDS | 30.0% | |
| ! | ATTCGAGGAAGAAATTTTGA+TGG | + | chr1.3:77686949-77686968 | None:intergenic | 30.0% |
| !!! | AGTTTTGACTTCTGGTTTAT+GGG | - | chr1.3:77687089-77687108 | MS.gene037374:CDS | 30.0% |
| !!! | TAGTTTTGACTTCTGGTTTA+TGG | - | chr1.3:77687088-77687107 | MS.gene037374:CDS | 30.0% |
| AAGATTACATACCATGCTCT+CGG | + | chr1.3:77686562-77686581 | None:intergenic | 35.0% | |
| ATACAGTAAAAGGGAAACTG+AGG | - | chr1.3:77686745-77686764 | MS.gene037374:intron | 35.0% | |
| GATGAACATGTTTGGAAGAA+AGG | - | chr1.3:77686514-77686533 | MS.gene037374:CDS | 35.0% | |
| TCTAATGAATTCTCCATTCG+AGG | + | chr1.3:77686964-77686983 | None:intergenic | 35.0% | |
| ACAGAAAGCAACTTTGTGAG+TGG | + | chr1.3:77686916-77686935 | None:intergenic | 40.0% | |
| AGATATCTGCCTGATTCTTC+TGG | - | chr1.3:77686340-77686359 | MS.gene037374:CDS | 40.0% | |
| AGGCAGATATCTCTTTGATG+AGG | + | chr1.3:77686332-77686351 | None:intergenic | 40.0% | |
| AGGCTATCTCTAAATCTCAG+AGG | - | chr1.3:77687150-77687169 | MS.gene037374:CDS | 40.0% | |
| AGTAAAAGGGAAACTGAGGT+TGG | - | chr1.3:77686749-77686768 | MS.gene037374:intron | 40.0% | |
| CACAAAGTCAAAGCAAGGTA+TGG | - | chr1.3:77686381-77686400 | MS.gene037374:CDS | 40.0% | |
| CTCTAAATCTCAGAGGGAAA+AGG | - | chr1.3:77687157-77687176 | MS.gene037374:CDS | 40.0% | |
| CTGTATATGCCAGAAGAATC+AGG | + | chr1.3:77686352-77686371 | None:intergenic | 40.0% | |
| GGCTATCTCTAAATCTCAGA+GGG | - | chr1.3:77687151-77687170 | MS.gene037374:CDS | 40.0% | |
| GTAAAAGGGAAACTGAGGTT+GGG | - | chr1.3:77686750-77686769 | MS.gene037374:intron | 40.0% | |
| TCCATCACAAAGTCAAAGCA+AGG | - | chr1.3:77686376-77686395 | MS.gene037374:CDS | 40.0% | |
| TGTGAGTGGAAATGAAGAGA+AGG | + | chr1.3:77686902-77686921 | None:intergenic | 40.0% | |
| ! | ACCTTGCTTTGACTTTGTGA+TGG | + | chr1.3:77686380-77686399 | None:intergenic | 40.0% |
| ! | GAGAGCATGGTATGTAATCT+TGG | - | chr1.3:77686561-77686580 | MS.gene037374:CDS | 40.0% |
| ! | TGTTACTTATCAACCACGTC+AGG | - | chr1.3:77686864-77686883 | MS.gene037374:intron | 40.0% |
| !! | AGCTTTAAGATATCTCCAGC+AGG | - | chr1.3:77687130-77687149 | MS.gene037374:CDS | 40.0% |
| !!! | CTGTGGATAGTTTTGACTTC+TGG | - | chr1.3:77687081-77687100 | MS.gene037374:CDS | 40.0% |
| ATGAAGAGAAGGCCAGCTAT+AGG | + | chr1.3:77686891-77686910 | None:intergenic | 45.0% | |
| GAGATTTAGAGATAGCCTGC+TGG | + | chr1.3:77687148-77687167 | None:intergenic | 45.0% | |
| TTGTAACCTGCAGTGAGACT+TGG | - | chr1.3:77686708-77686727 | MS.gene037374:CDS | 45.0% | |
| ! | AAGGGTGACAGTTTTGCACA+TGG | - | chr1.3:77686533-77686552 | MS.gene037374:CDS | 45.0% |
| GGAGCCAGAATTCTTCAGGT+TGG | - | chr1.3:77686771-77686790 | MS.gene037374:intron | 50.0% | |
| GTTGGGAGCCAGAATTCTTC+AGG | - | chr1.3:77686767-77686786 | MS.gene037374:intron | 50.0% | |
| TTTGGTCCAAGTCTCACTGC+AGG | + | chr1.3:77686717-77686736 | None:intergenic | 50.0% | |
| !! | CAGAATTCTTCAGGTTGGCG+GGG | - | chr1.3:77686776-77686795 | MS.gene037374:intron | 50.0% |
| !! | CCAGAATTCTTCAGGTTGGC+GGG | - | chr1.3:77686775-77686794 | MS.gene037374:intron | 50.0% |
| !! | GCCAGAATTCTTCAGGTTGG+CGG | - | chr1.3:77686774-77686793 | MS.gene037374:intron | 50.0% |
| ACCACGTCAGGTCCTATAGC+TGG | - | chr1.3:77686876-77686895 | MS.gene037374:intron | 55.0% | |
| CCCGCCAACCTGAAGAATTC+TGG | + | chr1.3:77686778-77686797 | None:intergenic | 55.0% | |
| GCACATGGAGTCCGAGAGCA+TGG | - | chr1.3:77686548-77686567 | MS.gene037374:CDS | 60.0% | |
| GCCAGCTATAGGACCTGACG+TGG | + | chr1.3:77686880-77686899 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 77686283 | 77687184 | 77686283 | ID=MS.gene037374 |
| chr1.3 | mRNA | 77686283 | 77687184 | 77686283 | ID=MS.gene037374.t1;Parent=MS.gene037374 |
| chr1.3 | exon | 77687070 | 77687184 | 77687070 | ID=MS.gene037374.t1.exon1;Parent=MS.gene037374.t1 |
| chr1.3 | CDS | 77687070 | 77687184 | 77687070 | ID=cds.MS.gene037374.t1;Parent=MS.gene037374.t1 |
| chr1.3 | exon | 77686898 | 77686978 | 77686898 | ID=MS.gene037374.t1.exon2;Parent=MS.gene037374.t1 |
| chr1.3 | CDS | 77686898 | 77686978 | 77686898 | ID=cds.MS.gene037374.t1;Parent=MS.gene037374.t1 |
| chr1.3 | exon | 77686283 | 77686746 | 77686283 | ID=MS.gene037374.t1.exon3;Parent=MS.gene037374.t1 |
| chr1.3 | CDS | 77686283 | 77686746 | 77686283 | ID=cds.MS.gene037374.t1;Parent=MS.gene037374.t1 |
| Gene Sequence |
| Protein sequence |
