Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061035.t1 | XP_013466677.1 | 83 | 223 | 27 | 1 | 1 | 212 | 1 | 223 | 1.10E-88 | 336.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061035.t1 | Q9FTA0 | 58.0 | 176 | 69 | 1 | 37 | 212 | 45 | 215 | 6.1e-53 | 208.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061035.t1 | A0A072VGZ4 | 83.0 | 223 | 27 | 1 | 1 | 212 | 1 | 223 | 7.6e-89 | 336.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene061035 | MS.gene040857 | PPI |
| MS.gene93121 | MS.gene061035 | PPI |
| MS.gene93119 | MS.gene061035 | PPI |
| MS.gene061035 | MS.gene037374 | PPI |
| MS.gene026428 | MS.gene061035 | PPI |
| MS.gene061035 | MS.gene93119 | PPI |
| MS.gene061035 | MS.gene037375 | PPI |
| MS.gene037373 | MS.gene061035 | PPI |
| MS.gene061654 | MS.gene061035 | PPI |
| MS.gene93120 | MS.gene061035 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061035.t1 | MTR_1g033750 | 88.789 | 223 | 14 | 1 | 1 | 212 | 1 | 223 | 8.35e-144 | 400 |
| MS.gene061035.t1 | MTR_1g114640 | 65.455 | 220 | 64 | 2 | 2 | 211 | 3 | 220 | 8.17e-99 | 286 |
| MS.gene061035.t1 | MTR_1g114690 | 57.767 | 206 | 71 | 2 | 12 | 211 | 22 | 217 | 1.93e-78 | 234 |
| MS.gene061035.t1 | MTR_1g114690 | 57.005 | 207 | 73 | 2 | 12 | 212 | 22 | 218 | 1.34e-77 | 232 |
| MS.gene061035.t1 | MTR_1g114750 | 56.500 | 200 | 83 | 2 | 13 | 212 | 26 | 221 | 1.43e-76 | 230 |
| MS.gene061035.t1 | MTR_1g114640 | 65.680 | 169 | 46 | 2 | 2 | 160 | 3 | 169 | 1.75e-70 | 213 |
| MS.gene061035.t1 | MTR_1g114690 | 48.963 | 241 | 73 | 3 | 12 | 212 | 22 | 252 | 4.70e-70 | 214 |
| MS.gene061035.t1 | MTR_1g115130 | 65.772 | 149 | 51 | 0 | 64 | 212 | 46 | 194 | 1.11e-66 | 204 |
| MS.gene061035.t1 | MTR_8g100065 | 51.366 | 183 | 88 | 1 | 31 | 212 | 3 | 185 | 9.54e-63 | 193 |
| MS.gene061035.t1 | MTR_8g100065 | 56.442 | 163 | 70 | 1 | 51 | 212 | 45 | 207 | 1.99e-61 | 191 |
| MS.gene061035.t1 | MTR_6g077990 | 47.525 | 202 | 99 | 2 | 11 | 212 | 5 | 199 | 3.71e-59 | 185 |
| MS.gene061035.t1 | MTR_6g077990 | 54.321 | 162 | 74 | 0 | 51 | 212 | 37 | 198 | 5.32e-59 | 184 |
| MS.gene061035.t1 | MTR_6g077990 | 56.376 | 149 | 65 | 0 | 64 | 212 | 8 | 156 | 5.79e-56 | 175 |
| MS.gene061035.t1 | MTR_1g114690 | 49.198 | 187 | 67 | 4 | 12 | 192 | 22 | 186 | 1.61e-49 | 160 |
| MS.gene061035.t1 | MTR_1g114680 | 69.369 | 111 | 34 | 0 | 102 | 212 | 53 | 163 | 7.26e-47 | 153 |
| MS.gene061035.t1 | MTR_7g083570 | 37.714 | 175 | 109 | 0 | 37 | 211 | 102 | 276 | 1.12e-41 | 142 |
| MS.gene061035.t1 | MTR_3g100750 | 40.000 | 155 | 92 | 1 | 55 | 209 | 102 | 255 | 9.95e-40 | 137 |
| MS.gene061035.t1 | MTR_8g069110 | 37.430 | 179 | 101 | 2 | 41 | 209 | 90 | 267 | 1.48e-38 | 134 |
| MS.gene061035.t1 | MTR_8g100065 | 48.819 | 127 | 64 | 1 | 31 | 156 | 3 | 129 | 3.86e-36 | 124 |
| MS.gene061035.t1 | MTR_6g077990 | 53.704 | 108 | 50 | 0 | 51 | 158 | 37 | 144 | 6.89e-36 | 124 |
| MS.gene061035.t1 | MTR_8g100065 | 56.075 | 107 | 46 | 1 | 51 | 156 | 45 | 151 | 5.32e-35 | 121 |
| MS.gene061035.t1 | MTR_2g076350 | 36.788 | 193 | 113 | 3 | 28 | 211 | 35 | 227 | 2.02e-34 | 122 |
| MS.gene061035.t1 | MTR_5g015750 | 48.611 | 72 | 25 | 2 | 2 | 63 | 3 | 72 | 4.04e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061035.t1 | AT5G08350 | 55.051 | 198 | 82 | 2 | 17 | 212 | 23 | 215 | 4.42e-73 | 221 |
| MS.gene061035.t1 | AT5G23370 | 61.111 | 162 | 63 | 0 | 51 | 212 | 55 | 216 | 3.09e-70 | 213 |
| MS.gene061035.t1 | AT5G23350 | 59.748 | 159 | 64 | 0 | 51 | 209 | 55 | 213 | 1.00e-68 | 209 |
| MS.gene061035.t1 | AT5G23360 | 56.790 | 162 | 70 | 0 | 51 | 212 | 47 | 208 | 3.42e-67 | 205 |
| MS.gene061035.t1 | AT4G01600 | 37.209 | 215 | 121 | 4 | 12 | 212 | 13 | 227 | 1.50e-43 | 146 |
| MS.gene061035.t1 | AT4G01600 | 39.227 | 181 | 102 | 2 | 40 | 212 | 8 | 188 | 1.99e-42 | 142 |
| MS.gene061035.t1 | AT5G13200 | 37.059 | 170 | 107 | 0 | 41 | 210 | 92 | 261 | 2.89e-41 | 141 |
| MS.gene061035.t1 | AT4G01600 | 36.967 | 211 | 122 | 5 | 12 | 212 | 13 | 222 | 1.32e-39 | 135 |
| MS.gene061035.t1 | AT1G28200 | 35.849 | 159 | 101 | 1 | 51 | 209 | 97 | 254 | 2.67e-36 | 128 |
| MS.gene061035.t1 | AT1G28200 | 35.849 | 159 | 101 | 1 | 51 | 209 | 97 | 254 | 2.67e-36 | 128 |
| MS.gene061035.t1 | AT2G22475 | 34.545 | 165 | 107 | 1 | 45 | 209 | 128 | 291 | 1.51e-35 | 127 |
| MS.gene061035.t1 | AT5G13200 | 36.207 | 116 | 74 | 0 | 41 | 156 | 92 | 207 | 5.33e-23 | 92.4 |
| MS.gene061035.t1 | AT4G40100 | 29.891 | 184 | 95 | 3 | 29 | 209 | 64 | 216 | 4.91e-21 | 87.4 |
| MS.gene061035.t1 | AT2G22475 | 30.435 | 115 | 79 | 1 | 45 | 159 | 128 | 241 | 2.09e-16 | 75.5 |
Find 31 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCAATTTCAATGTACTTT+TGG | 0.206367 | 1.4:+18415299 | None:intergenic |
| GTTCTAATAAAGTGACCTTT+TGG | 0.297999 | 1.4:+18415568 | None:intergenic |
| CTCACTAGGATGAACAATCT+TGG | 0.396062 | 1.4:-18422414 | MS.gene061035:CDS |
| AATTGATCTCTCACTACAAA+AGG | 0.403914 | 1.4:+18415603 | None:intergenic |
| TTTAACAATGTAGTGAAACT+TGG | 0.423527 | 1.4:-18422027 | MS.gene061035:intron |
| ACTTTGATTTGAACTTGTGC+TGG | 0.436691 | 1.4:+18422595 | None:intergenic |
| ATTAAAATCTCTTCTCCAAA+AGG | 0.447843 | 1.4:-18415583 | MS.gene061035:CDS |
| GCTGGACTTATTGTAGTAGT+TGG | 0.453049 | 1.4:+18422613 | None:intergenic |
| TCACTAGGATGAACAATCTT+GGG | 0.454379 | 1.4:-18422413 | MS.gene061035:CDS |
| CTTATTGTAGTAGTTGGACT+TGG | 0.455357 | 1.4:+18422619 | None:intergenic |
| TTATTCCACTTGGCAAGATA+AGG | 0.462580 | 1.4:-18415357 | MS.gene061035:CDS |
| TGTTATCTATCAACAACATC+TGG | 0.466437 | 1.4:-18415667 | MS.gene061035:CDS |
| GGAGCTAGAATTCTTCAAGT+TGG | 0.473121 | 1.4:-18421964 | MS.gene061035:CDS |
| TCAAGTTGGAGGAGTGGAAA+AGG | 0.489991 | 1.4:-18421950 | MS.gene061035:intron |
| CAACTATTTAGTGTAAAAGA+TGG | 0.490960 | 1.4:-18415715 | MS.gene061035:CDS |
| TTTCTTCAAGAGCTATTCGT+TGG | 0.492879 | 1.4:-18422674 | MS.gene061035:CDS |
| TTAACAATGTAGTGAAACTT+GGG | 0.511880 | 1.4:-18422026 | MS.gene061035:intron |
| GCTAGAATTCTTCAAGTTGG+AGG | 0.512754 | 1.4:-18421961 | MS.gene061035:CDS |
| CTGAAAGGAAAGCTGAGCTT+AGG | 0.525427 | 1.4:-18421985 | MS.gene061035:CDS |
| ACAACATCTGGTCCTCTAGC+AGG | 0.532817 | 1.4:-18415655 | MS.gene061035:CDS |
| CTTTATTAGAACATACTACA+AGG | 0.557502 | 1.4:-18415557 | MS.gene061035:intron |
| TTGTAGGTCATTATTCCACT+TGG | 0.560547 | 1.4:-18415367 | MS.gene061035:intron |
| AATTCTTCAAGTTGGAGGAG+TGG | 0.572161 | 1.4:-18421956 | MS.gene061035:CDS |
| GCAACGGGACTTAAAGAACA+TGG | 0.586634 | 1.4:-18422372 | MS.gene061035:intron |
| GAGCAAATTCAGTTCTCACT+AGG | 0.589590 | 1.4:-18422428 | MS.gene061035:CDS |
| AAAGTACATTGAAATTGCTA+CGG | 0.602017 | 1.4:-18415296 | MS.gene061035:CDS |
| ACAAACCTTATCTTGCCAAG+TGG | 0.607201 | 1.4:+18415352 | None:intergenic |
| AACATAACTGATACACTGAA+AGG | 0.613918 | 1.4:-18422000 | MS.gene061035:CDS |
| GTACATTGAAATTGCTACGG+TGG | 0.620058 | 1.4:-18415293 | MS.gene061035:CDS |
| TTAGTGTAAAAGATGGAGAG+AGG | 0.661609 | 1.4:-18415708 | MS.gene061035:CDS |
| ATGAAGAGTAGACCTGCTAG+AGG | 0.665363 | 1.4:+18415643 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 18415210 | 18422705 | 18415210 | ID=MS.gene061035 |
| chr1.4 | mRNA | 18415210 | 18422705 | 18415210 | ID=MS.gene061035.t1;Parent=MS.gene061035 |
| chr1.4 | exon | 18422597 | 18422705 | 18422597 | ID=MS.gene061035.t1.exon1;Parent=MS.gene061035.t1 |
| chr1.4 | CDS | 18422597 | 18422705 | 18422597 | ID=cds.MS.gene061035.t1;Parent=MS.gene061035.t1 |
| chr1.4 | exon | 18422373 | 18422453 | 18422373 | ID=MS.gene061035.t1.exon2;Parent=MS.gene061035.t1 |
| chr1.4 | CDS | 18422373 | 18422453 | 18422373 | ID=cds.MS.gene061035.t1;Parent=MS.gene061035.t1 |
| chr1.4 | exon | 18421951 | 18422036 | 18421951 | ID=MS.gene061035.t1.exon3;Parent=MS.gene061035.t1 |
| chr1.4 | CDS | 18421951 | 18422036 | 18421951 | ID=cds.MS.gene061035.t1;Parent=MS.gene061035.t1 |
| chr1.4 | exon | 18415558 | 18415746 | 18415558 | ID=MS.gene061035.t1.exon4;Parent=MS.gene061035.t1 |
| chr1.4 | CDS | 18415558 | 18415746 | 18415558 | ID=cds.MS.gene061035.t1;Parent=MS.gene061035.t1 |
| chr1.4 | exon | 18415210 | 18415383 | 18415210 | ID=MS.gene061035.t1.exon5;Parent=MS.gene061035.t1 |
| chr1.4 | CDS | 18415210 | 18415383 | 18415210 | ID=cds.MS.gene061035.t1;Parent=MS.gene061035.t1 |
| Gene Sequence |
| Protein sequence |
