Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93120.t1 | XP_004497247.1 | 83.6 | 220 | 35 | 1 | 1 | 220 | 1 | 219 | 3.10E-99 | 371.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93120.t1 | Q9FMW4 | 58.1 | 203 | 84 | 1 | 16 | 218 | 18 | 219 | 5.9e-59 | 228.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93120.t1 | A0A1S2Y1F9 | 83.6 | 220 | 35 | 1 | 1 | 220 | 1 | 219 | 2.2e-99 | 371.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054142 | MS.gene93120 | 0.833714 | 4.55E-56 | -1.69E-46 |
| MS.gene057102 | MS.gene93120 | 0.815768 | 7.81E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene93120 | -0.805831 | 1.10E-49 | -1.69E-46 |
| MS.gene061100 | MS.gene93120 | 0.817552 | 3.11E-52 | -1.69E-46 |
| MS.gene061188 | MS.gene93120 | 0.816569 | 5.17E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene93120 | MS.gene037374 | PPI |
| MS.gene037373 | MS.gene93120 | PPI |
| MS.gene061654 | MS.gene93120 | PPI |
| MS.gene93120 | MS.gene037375 | PPI |
| MS.gene93120 | MS.gene93119 | PPI |
| MS.gene93121 | MS.gene93120 | PPI |
| MS.gene026428 | MS.gene93120 | PPI |
| MS.gene93120 | MS.gene040857 | PPI |
| MS.gene93120 | MS.gene061035 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93120.t1 | MTR_1g114640 | 83.105 | 219 | 29 | 2 | 1 | 212 | 1 | 218 | 2.23e-131 | 369 |
| MS.gene93120.t1 | MTR_1g033750 | 63.636 | 220 | 71 | 3 | 5 | 215 | 4 | 223 | 7.37e-95 | 276 |
| MS.gene93120.t1 | MTR_1g114640 | 80.723 | 166 | 24 | 2 | 1 | 159 | 1 | 165 | 1.76e-90 | 263 |
| MS.gene93120.t1 | MTR_1g114690 | 53.070 | 228 | 84 | 3 | 1 | 215 | 1 | 218 | 3.25e-77 | 231 |
| MS.gene93120.t1 | MTR_1g114690 | 51.982 | 227 | 86 | 3 | 1 | 214 | 1 | 217 | 4.93e-75 | 226 |
| MS.gene93120.t1 | MTR_1g114750 | 48.214 | 224 | 104 | 2 | 1 | 215 | 1 | 221 | 1.41e-70 | 215 |
| MS.gene93120.t1 | MTR_1g114690 | 46.565 | 262 | 83 | 4 | 1 | 215 | 1 | 252 | 5.12e-70 | 214 |
| MS.gene93120.t1 | MTR_1g114680 | 93.103 | 116 | 8 | 0 | 105 | 220 | 53 | 168 | 2.25e-68 | 208 |
| MS.gene93120.t1 | MTR_8g100065 | 58.896 | 163 | 66 | 1 | 54 | 215 | 23 | 185 | 4.32e-63 | 194 |
| MS.gene93120.t1 | MTR_6g077990 | 55.625 | 160 | 71 | 0 | 58 | 217 | 42 | 201 | 1.04e-62 | 194 |
| MS.gene93120.t1 | MTR_6g077990 | 52.000 | 175 | 84 | 0 | 43 | 217 | 26 | 200 | 1.05e-62 | 194 |
| MS.gene93120.t1 | MTR_1g115130 | 60.000 | 150 | 60 | 0 | 67 | 216 | 46 | 195 | 1.25e-62 | 194 |
| MS.gene93120.t1 | MTR_8g100065 | 58.896 | 163 | 66 | 1 | 54 | 215 | 45 | 207 | 1.65e-62 | 194 |
| MS.gene93120.t1 | MTR_6g077990 | 55.629 | 151 | 67 | 0 | 67 | 217 | 8 | 158 | 1.55e-58 | 182 |
| MS.gene93120.t1 | MTR_1g114690 | 50.867 | 173 | 62 | 3 | 1 | 160 | 1 | 163 | 2.37e-48 | 157 |
| MS.gene93120.t1 | MTR_7g083570 | 35.052 | 194 | 126 | 0 | 20 | 213 | 82 | 275 | 4.38e-43 | 147 |
| MS.gene93120.t1 | MTR_3g100750 | 32.212 | 208 | 130 | 2 | 15 | 212 | 49 | 255 | 8.09e-42 | 142 |
| MS.gene93120.t1 | MTR_8g069110 | 35.359 | 181 | 106 | 2 | 42 | 212 | 88 | 267 | 4.66e-41 | 141 |
| MS.gene93120.t1 | MTR_6g077990 | 51.240 | 121 | 59 | 0 | 43 | 163 | 26 | 146 | 3.50e-39 | 132 |
| MS.gene93120.t1 | MTR_8g100065 | 59.813 | 107 | 42 | 1 | 54 | 159 | 23 | 129 | 1.22e-38 | 130 |
| MS.gene93120.t1 | MTR_8g100065 | 59.813 | 107 | 42 | 1 | 54 | 159 | 45 | 151 | 4.13e-38 | 130 |
| MS.gene93120.t1 | MTR_2g076350 | 33.491 | 212 | 134 | 3 | 6 | 214 | 20 | 227 | 1.56e-33 | 120 |
| MS.gene93120.t1 | MTR_5g015750 | 76.712 | 73 | 9 | 2 | 1 | 66 | 1 | 72 | 7.69e-31 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93120.t1 | AT5G23370 | 59.000 | 200 | 81 | 1 | 16 | 215 | 18 | 216 | 6.17e-80 | 238 |
| MS.gene93120.t1 | AT5G08350 | 57.000 | 200 | 74 | 3 | 21 | 215 | 23 | 215 | 3.80e-76 | 229 |
| MS.gene93120.t1 | AT5G23350 | 55.072 | 207 | 82 | 3 | 16 | 217 | 18 | 218 | 4.02e-75 | 226 |
| MS.gene93120.t1 | AT5G23360 | 59.756 | 164 | 66 | 0 | 54 | 217 | 47 | 210 | 1.02e-72 | 220 |
| MS.gene93120.t1 | AT4G01600 | 40.909 | 176 | 103 | 1 | 42 | 216 | 14 | 189 | 5.01e-48 | 156 |
| MS.gene93120.t1 | AT4G01600 | 40.909 | 176 | 103 | 1 | 42 | 216 | 53 | 228 | 1.60e-47 | 156 |
| MS.gene93120.t1 | AT5G13200 | 36.170 | 188 | 120 | 0 | 29 | 216 | 77 | 264 | 2.96e-44 | 149 |
| MS.gene93120.t1 | AT4G01600 | 43.709 | 151 | 84 | 1 | 67 | 216 | 73 | 223 | 5.11e-43 | 145 |
| MS.gene93120.t1 | AT1G28200 | 35.678 | 199 | 116 | 4 | 22 | 212 | 60 | 254 | 6.17e-42 | 143 |
| MS.gene93120.t1 | AT1G28200 | 35.678 | 199 | 116 | 4 | 22 | 212 | 60 | 254 | 6.17e-42 | 143 |
| MS.gene93120.t1 | AT2G22475 | 35.079 | 191 | 121 | 2 | 23 | 212 | 103 | 291 | 7.77e-42 | 144 |
| MS.gene93120.t1 | AT5G13200 | 33.333 | 132 | 88 | 0 | 29 | 160 | 77 | 208 | 2.11e-25 | 99.0 |
| MS.gene93120.t1 | AT4G40100 | 27.368 | 190 | 103 | 2 | 23 | 212 | 62 | 216 | 4.53e-22 | 90.5 |
| MS.gene93120.t1 | AT2G22475 | 31.757 | 148 | 98 | 2 | 23 | 169 | 103 | 248 | 6.63e-22 | 90.5 |
Find 42 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTTTCGACTTCTGGTTTA+TGG | 0.139805 | 1.1:-80518655 | MS.gene93120:CDS |
| TTTCTTCATGAGTTACTTAA+TGG | 0.146739 | 1.1:-80519448 | MS.gene93120:CDS |
| CTATGGATGGTTTCGACTTC+TGG | 0.198951 | 1.1:-80518662 | MS.gene93120:CDS |
| AAGATAACAGATACAGTAAA+AGG | 0.228633 | 1.1:-80519008 | MS.gene93120:CDS |
| TTTACTGTATCTGTTATCTT+TGG | 0.247894 | 1.1:+80519011 | None:intergenic |
| GGTTTCGACTTCTGGTTTAT+GGG | 0.291409 | 1.1:-80518654 | MS.gene93120:CDS |
| GTTGGGAGCCAGAATTCTTC+AGG | 0.336328 | 1.1:-80518976 | MS.gene93120:CDS |
| CCCGCCAACCTGAAGAATTC+TGG | 0.351542 | 1.1:+80518968 | None:intergenic |
| GATGAACATGTTTGGAAGAA+AGG | 0.373892 | 1.1:-80519229 | MS.gene93120:CDS |
| ATTCATTAGAGTTCACTATA+AGG | 0.401080 | 1.1:-80518769 | MS.gene93120:CDS |
| TATCTGCCAGCTGATTCTTC+TGG | 0.418036 | 1.1:-80519400 | MS.gene93120:CDS |
| ATGACTAAGATGAACATGTT+TGG | 0.423883 | 1.1:-80519237 | MS.gene93120:CDS |
| ATGAACATGTTTGGAAGAAA+GGG | 0.430526 | 1.1:-80519228 | MS.gene93120:CDS |
| ATACATAGAAATAGTCACTA+TGG | 0.430727 | 1.1:-80518679 | MS.gene93120:CDS |
| CCAGAATTCTTCAGGTTGGC+GGG | 0.441065 | 1.1:-80518968 | MS.gene93120:CDS |
| GCCAGAATTCTTCAGGTTGG+CGG | 0.449134 | 1.1:-80518969 | MS.gene93120:CDS |
| CTCTAAATCTCAGAGGGAAA+AGG | 0.451562 | 1.1:-80518586 | MS.gene93120:CDS |
| ATCAAAATTTCTTCCTCGAA+TGG | 0.453468 | 1.1:-80518795 | MS.gene93120:CDS |
| ACCTTGCTTTGACTTTGTGA+TGG | 0.474385 | 1.1:+80519363 | None:intergenic |
| TCTAATGAATTCTCCATTCG+AGG | 0.480167 | 1.1:+80518782 | None:intergenic |
| ATGAAGAGAAGGCCAGCTAT+AGG | 0.493362 | 1.1:+80518855 | None:intergenic |
| AGCTTTAAGATATCTTCAGC+AGG | 0.515990 | 1.1:-80518613 | MS.gene93120:CDS |
| AGTAAAAGGGAAACTGAGGT+TGG | 0.524536 | 1.1:-80518994 | MS.gene93120:CDS |
| TTGTAACCTGCAGTGAGACT+TGG | 0.527828 | 1.1:-80519035 | MS.gene93120:intron |
| ACCACGTCAGGTCCTATAGC+TGG | 0.538413 | 1.1:-80518867 | MS.gene93120:CDS |
| AGATAACAGATACAGTAAAA+GGG | 0.540258 | 1.1:-80519007 | MS.gene93120:CDS |
| GGAGCCAGAATTCTTCAGGT+TGG | 0.551383 | 1.1:-80518972 | MS.gene93120:CDS |
| GGCTATCTCTAAATCTCAGA+GGG | 0.564713 | 1.1:-80518592 | MS.gene93120:CDS |
| GCACATGGAGTCCGAGAGCA+TGG | 0.566367 | 1.1:-80519195 | MS.gene93120:intron |
| GTAAAAGGGAAACTGAGGTT+GGG | 0.569074 | 1.1:-80518993 | MS.gene93120:CDS |
| TGTTACTTATCAACCACGTC+AGG | 0.572611 | 1.1:-80518879 | MS.gene93120:CDS |
| ATAGAAATAGTCACTATGGA+TGG | 0.592517 | 1.1:-80518675 | MS.gene93120:CDS |
| TCCATCACAAAGTCAAAGCA+AGG | 0.594218 | 1.1:-80519364 | MS.gene93120:intron |
| AAGATTACATACCATGCTCT+CGG | 0.595383 | 1.1:+80519184 | None:intergenic |
| ACAGAAAGCAACTTTGTGAG+TGG | 0.605272 | 1.1:+80518830 | None:intergenic |
| TGTGAGTGGAAATGAAGAGA+AGG | 0.611200 | 1.1:+80518844 | None:intergenic |
| CAGAATTCTTCAGGTTGGCG+GGG | 0.646633 | 1.1:-80518967 | MS.gene93120:CDS |
| TGGCAGATATCTCTTTGATG+AGG | 0.656890 | 1.1:+80519414 | None:intergenic |
| ATACAGTAAAAGGGAAACTG+AGG | 0.664591 | 1.1:-80518998 | MS.gene93120:CDS |
| TATATGCCAGAAGAATCAGC+TGG | 0.671059 | 1.1:+80519394 | None:intergenic |
| AGGCTATCTCTAAATCTCAG+AGG | 0.678214 | 1.1:-80518593 | MS.gene93120:CDS |
| GCCAGCTATAGGACCTGACG+TGG | 0.697419 | 1.1:+80518866 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTCTATGTATTTTTCTGA+TGG | + | chr1.1:80519354-80519373 | None:intergenic | 20.0% |
| !!! | TGAAATAATACAGTTTTGTT+AGG | + | chr1.1:80518713-80518732 | None:intergenic | 20.0% |
| ! | AAGATAACAGATACAGTAAA+AGG | - | chr1.1:80519033-80519052 | MS.gene93120:intron | 25.0% |
| ! | AGATAACAGATACAGTAAAA+GGG | - | chr1.1:80519034-80519053 | MS.gene93120:intron | 25.0% |
| ! | ATACATAGAAATAGTCACTA+TGG | - | chr1.1:80519362-80519381 | MS.gene93120:intron | 25.0% |
| ! | ATTCATTAGAGTTCACTATA+AGG | - | chr1.1:80519272-80519291 | MS.gene93120:intron | 25.0% |
| ! | TAGAATGAAGTAGTAATTGA+TGG | - | chr1.1:80518970-80518989 | MS.gene93120:CDS | 25.0% |
| ! | TTTACTGTATCTGTTATCTT+TGG | + | chr1.1:80519033-80519052 | None:intergenic | 25.0% |
| ! | TTTCTTCATGAGTTACTTAA+TGG | - | chr1.1:80518593-80518612 | MS.gene93120:CDS | 25.0% |
| !! | ATGATGCATCATACATATTT+TGG | + | chr1.1:80518926-80518945 | None:intergenic | 25.0% |
| !! | ATGATGCATCATTTGAAATT+TGG | - | chr1.1:80518934-80518953 | MS.gene93120:CDS | 25.0% |
| !! | CATTTGAAATTTGGAATGAT+TGG | - | chr1.1:80518943-80518962 | MS.gene93120:CDS | 25.0% |
| !! | TTTAATGTCATTGATGATCA+TGG | - | chr1.1:80518747-80518766 | MS.gene93120:CDS | 25.0% |
| !!! | ACTTGTTGTATTTTTTCATG+TGG | + | chr1.1:80519306-80519325 | None:intergenic | 25.0% |
| !!! | GTTTCATCATATTTTGACTT+TGG | + | chr1.1:80519332-80519351 | None:intergenic | 25.0% |
| ATAGAAATAGTCACTATGGA+TGG | - | chr1.1:80519366-80519385 | MS.gene93120:CDS | 30.0% | |
| ATCAAAATTTCTTCCTCGAA+TGG | - | chr1.1:80519246-80519265 | MS.gene93120:CDS | 30.0% | |
| ATGAACATGTTTGGAAGAAA+GGG | - | chr1.1:80518813-80518832 | MS.gene93120:CDS | 30.0% | |
| ATGACTAAGATGAACATGTT+TGG | - | chr1.1:80518804-80518823 | MS.gene93120:CDS | 30.0% | |
| ! | ATTCGAGGAAGAAATTTTGA+TGG | + | chr1.1:80519247-80519266 | None:intergenic | 30.0% |
| ! | CATGGTCATTTCATATTTTC+AGG | - | chr1.1:80518765-80518784 | MS.gene93120:CDS | 30.0% |
| AAGATTACATACCATGCTCT+CGG | + | chr1.1:80518860-80518879 | None:intergenic | 35.0% | |
| ATACAGTAAAAGGGAAACTG+AGG | - | chr1.1:80519043-80519062 | MS.gene93120:intron | 35.0% | |
| GATGAACATGTTTGGAAGAA+AGG | - | chr1.1:80518812-80518831 | MS.gene93120:CDS | 35.0% | |
| TCTAATGAATTCTCCATTCG+AGG | + | chr1.1:80519262-80519281 | None:intergenic | 35.0% | |
| !! | AGCTTTAAGATATCTTCAGC+AGG | - | chr1.1:80519428-80519447 | MS.gene93120:CDS | 35.0% |
| ACAGAAAGCAACTTTGTGAG+TGG | + | chr1.1:80519214-80519233 | None:intergenic | 40.0% | |
| AGGCTATCTCTAAATCTCAG+AGG | - | chr1.1:80519448-80519467 | MS.gene93120:CDS | 40.0% | |
| AGTAAAAGGGAAACTGAGGT+TGG | - | chr1.1:80519047-80519066 | MS.gene93120:intron | 40.0% | |
| CACAAAGTCAAAGCAAGGTA+TGG | - | chr1.1:80518682-80518701 | MS.gene93120:CDS | 40.0% | |
| CTCTAAATCTCAGAGGGAAA+AGG | - | chr1.1:80519455-80519474 | MS.gene93120:CDS | 40.0% | |
| GGCTATCTCTAAATCTCAGA+GGG | - | chr1.1:80519449-80519468 | MS.gene93120:CDS | 40.0% | |
| GTAAAAGGGAAACTGAGGTT+GGG | - | chr1.1:80519048-80519067 | MS.gene93120:intron | 40.0% | |
| TATATGCCAGAAGAATCAGC+TGG | + | chr1.1:80518650-80518669 | None:intergenic | 40.0% | |
| TCCATCACAAAGTCAAAGCA+AGG | - | chr1.1:80518677-80518696 | MS.gene93120:CDS | 40.0% | |
| TGGCAGATATCTCTTTGATG+AGG | + | chr1.1:80518630-80518649 | None:intergenic | 40.0% | |
| TGTGAGTGGAAATGAAGAGA+AGG | + | chr1.1:80519200-80519219 | None:intergenic | 40.0% | |
| ! | ACCTTGCTTTGACTTTGTGA+TGG | + | chr1.1:80518681-80518700 | None:intergenic | 40.0% |
| ! | GAGAGCATGGTATGTAATCT+TGG | - | chr1.1:80518859-80518878 | MS.gene93120:CDS | 40.0% |
| ! | TGTTACTTATCAACCACGTC+AGG | - | chr1.1:80519162-80519181 | MS.gene93120:intron | 40.0% |
| !! | GGTTTCGACTTCTGGTTTAT+GGG | - | chr1.1:80519387-80519406 | MS.gene93120:CDS | 40.0% |
| !! | TGGTTTCGACTTCTGGTTTA+TGG | - | chr1.1:80519386-80519405 | MS.gene93120:CDS | 40.0% |
| ATGAAGAGAAGGCCAGCTAT+AGG | + | chr1.1:80519189-80519208 | None:intergenic | 45.0% | |
| CTATGGATGGTTTCGACTTC+TGG | - | chr1.1:80519379-80519398 | MS.gene93120:CDS | 45.0% | |
| TTGTAACCTGCAGTGAGACT+TGG | - | chr1.1:80519006-80519025 | MS.gene93120:CDS | 45.0% | |
| ! | AAGGGTGACAGTTTTGCACA+TGG | - | chr1.1:80518831-80518850 | MS.gene93120:CDS | 45.0% |
| ! | TATCTGCCAGCTGATTCTTC+TGG | - | chr1.1:80518641-80518660 | MS.gene93120:CDS | 45.0% |
| GGAGCCAGAATTCTTCAGGT+TGG | - | chr1.1:80519069-80519088 | MS.gene93120:intron | 50.0% | |
| GTTGGGAGCCAGAATTCTTC+AGG | - | chr1.1:80519065-80519084 | MS.gene93120:intron | 50.0% | |
| TTTGGTCCAAGTCTCACTGC+AGG | + | chr1.1:80519015-80519034 | None:intergenic | 50.0% | |
| !! | CAGAATTCTTCAGGTTGGCG+GGG | - | chr1.1:80519074-80519093 | MS.gene93120:intron | 50.0% |
| !! | CCAGAATTCTTCAGGTTGGC+GGG | - | chr1.1:80519073-80519092 | MS.gene93120:intron | 50.0% |
| !! | GCCAGAATTCTTCAGGTTGG+CGG | - | chr1.1:80519072-80519091 | MS.gene93120:intron | 50.0% |
| ACCACGTCAGGTCCTATAGC+TGG | - | chr1.1:80519174-80519193 | MS.gene93120:intron | 55.0% | |
| CCCGCCAACCTGAAGAATTC+TGG | + | chr1.1:80519076-80519095 | None:intergenic | 55.0% | |
| GCACATGGAGTCCGAGAGCA+TGG | - | chr1.1:80518846-80518865 | MS.gene93120:CDS | 60.0% | |
| GCCAGCTATAGGACCTGACG+TGG | + | chr1.1:80519178-80519197 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 80518581 | 80519482 | 80518581 | ID=MS.gene93120 |
| chr1.1 | mRNA | 80518581 | 80519482 | 80518581 | ID=MS.gene93120.t1;Parent=MS.gene93120 |
| chr1.1 | exon | 80519365 | 80519482 | 80519365 | ID=MS.gene93120.t1.exon1;Parent=MS.gene93120.t1 |
| chr1.1 | CDS | 80519365 | 80519482 | 80519365 | ID=cds.MS.gene93120.t1;Parent=MS.gene93120.t1 |
| chr1.1 | exon | 80519196 | 80519276 | 80519196 | ID=MS.gene93120.t1.exon2;Parent=MS.gene93120.t1 |
| chr1.1 | CDS | 80519196 | 80519276 | 80519196 | ID=cds.MS.gene93120.t1;Parent=MS.gene93120.t1 |
| chr1.1 | exon | 80518581 | 80519044 | 80518581 | ID=MS.gene93120.t1.exon3;Parent=MS.gene93120.t1 |
| chr1.1 | CDS | 80518581 | 80519044 | 80518581 | ID=cds.MS.gene93120.t1;Parent=MS.gene93120.t1 |
| Gene Sequence |
| Protein sequence |
