AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93119


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93119.t1 MTR_1g114640 83.957 187 30 0 1 187 1 187 1.97e-112 320
MS.gene93119.t1 MTR_1g114640 81.818 165 30 0 1 165 1 165 9.87e-96 276
MS.gene93119.t1 MTR_1g033750 59.804 204 79 2 5 205 4 207 1.32e-76 229
MS.gene93119.t1 MTR_1g114690 50.794 189 86 3 1 187 1 184 8.03e-55 174
MS.gene93119.t1 MTR_1g114690 49.198 187 92 2 1 187 1 184 3.50e-54 172
MS.gene93119.t1 MTR_1g114750 47.619 189 95 2 1 187 1 187 3.24e-53 170
MS.gene93119.t1 MTR_1g114690 43.498 223 85 4 1 187 1 218 6.23e-48 157
MS.gene93119.t1 MTR_6g077990 53.793 145 59 2 65 205 43 183 2.00e-46 152
MS.gene93119.t1 MTR_6g077990 53.793 145 59 2 65 205 42 182 2.09e-46 152
MS.gene93119.t1 MTR_1g114690 50.000 168 77 3 1 166 1 163 6.31e-46 151
MS.gene93119.t1 MTR_1g115130 42.927 205 90 3 1 205 1 178 1.31e-43 145
MS.gene93119.t1 MTR_8g100065 50.993 151 65 3 60 205 23 169 4.70e-43 143
MS.gene93119.t1 MTR_6g077990 54.015 137 55 2 73 205 8 140 1.57e-42 140
MS.gene93119.t1 MTR_8g100065 50.993 151 65 3 60 205 45 191 1.66e-42 142
MS.gene93119.t1 MTR_6g077990 58.095 105 44 0 65 169 42 146 4.82e-38 129
MS.gene93119.t1 MTR_8g100065 57.009 107 45 1 60 165 23 129 2.12e-36 124
MS.gene93119.t1 MTR_8g100065 57.009 107 45 1 60 165 45 151 5.59e-36 124
MS.gene93119.t1 MTR_5g015750 80.556 72 14 0 1 72 1 72 6.67e-35 120
MS.gene93119.t1 MTR_1g114680 85.714 77 11 0 111 187 53 129 3.17e-31 112
MS.gene93119.t1 MTR_7g083570 32.215 149 101 0 28 176 84 232 1.16e-24 98.2
MS.gene93119.t1 MTR_3g100750 35.766 137 81 3 71 202 109 243 2.73e-23 94.0
MS.gene93119.t1 MTR_8g069110 31.176 170 98 4 50 205 90 254 3.18e-22 91.7
MS.gene93119.t1 MTR_2g076350 41.176 102 59 1 46 146 52 153 7.78e-18 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93119.t1 AT5G08350 52.459 183 76 3 28 205 23 199 2.78e-55 175
MS.gene93119.t1 AT5G23370 48.293 205 101 1 1 205 1 200 4.00e-55 175
MS.gene93119.t1 AT5G23350 56.364 165 62 2 28 187 23 182 4.39e-54 172
MS.gene93119.t1 AT5G23360 47.525 202 87 4 1 197 1 188 3.35e-52 167
MS.gene93119.t1 AT4G01600 36.905 168 98 2 46 205 5 172 7.91e-30 109
MS.gene93119.t1 AT4G01600 34.343 198 121 3 16 205 15 211 1.54e-29 109
MS.gene93119.t1 AT4G01600 34.694 196 118 4 16 205 15 206 1.95e-26 101
MS.gene93119.t1 AT5G13200 31.373 153 105 0 35 187 77 229 6.04e-24 96.3
MS.gene93119.t1 AT1G28200 35.664 143 85 3 60 197 97 237 1.17e-22 92.4
MS.gene93119.t1 AT1G28200 35.664 143 85 3 60 197 97 237 1.17e-22 92.4
MS.gene93119.t1 AT5G13200 32.576 132 89 0 35 166 77 208 2.07e-22 90.9
MS.gene93119.t1 AT2G22475 32.895 152 91 4 59 205 133 278 6.11e-21 88.6
MS.gene93119.t1 AT2G22475 39.241 79 48 0 71 149 145 223 5.05e-16 74.3

Find 39 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTCACAAAGTTGCTTTC+TGG 0.165974 1.1:-80532771 MS.gene93119:CDS
TTCCTTCATGAGCTACTTAA+TGG 0.213634 1.1:-80533632 MS.gene93119:CDS
GCGTAGATGGTTTCGACTTC+TGG 0.218018 1.1:-80532515 MS.gene93119:CDS
AAGATAACAGATACAGTAAA+AGG 0.228633 1.1:-80532949 MS.gene93119:CDS
TTTACTGTATCTGTTATCTT+TGG 0.247894 1.1:+80532952 None:intergenic
ACTCTAATCAATTCTCCTTT+TGG 0.287784 1.1:+80532721 None:intergenic
AGATACTTGGCCGATTCTTC+TGG 0.307658 1.1:-80533566 MS.gene93119:CDS
ATACAGTAAAAGGGAAATTA+AGG 0.314014 1.1:-80532939 MS.gene93119:CDS
CTGACTAAGATGAACATGTT+TGG 0.338683 1.1:-80533203 MS.gene93119:CDS
TGTTACTTATCAACCACATT+AGG 0.368586 1.1:-80532820 MS.gene93119:CDS
TGACTAAGATGAACATGTTT+GGG 0.379362 1.1:-80533202 MS.gene93119:CDS
GATGAACATGTTTGGGAGAA+AGG 0.393124 1.1:-80533195 MS.gene93119:CDS
ATTGATTAGAGTTCTCTATA+AGG 0.399862 1.1:-80532710 MS.gene93119:intron
AGTAAAAGGGAAATTAAGGT+TGG 0.443877 1.1:-80532935 MS.gene93119:CDS
GTTGTATATGCCAGAAGAAT+CGG 0.444156 1.1:+80533556 None:intergenic
GGAGGCAAAATTATTCAAGT+TGG 0.454364 1.1:-80532913 MS.gene93119:CDS
ATGAACATGTTTGGGAGAAA+GGG 0.454867 1.1:-80533194 MS.gene93119:CDS
GGCAAAATTATTCAAGTTGG+AGG 0.471062 1.1:-80532910 MS.gene93119:CDS
ATCACCTCAACTCATTCAGT+TGG 0.473136 1.1:-80533602 MS.gene93119:CDS
ATAGAAATAGTCAGCGTAGA+TGG 0.474979 1.1:-80532528 MS.gene93119:CDS
ATGAAGAGGAGGCCAGCTAT+AGG 0.476519 1.1:+80532796 None:intergenic
CTGTAACCTGCAGTGAGACT+TGG 0.489945 1.1:-80532976 MS.gene93119:intron
ATCAAGATCTCGTCACCAAA+AGG 0.523407 1.1:-80532736 MS.gene93119:CDS
GTAAAAGGGAAATTAAGGTT+GGG 0.528971 1.1:-80532934 MS.gene93119:CDS
TCACCTCAACTCATTCAGTT+GGG 0.537734 1.1:-80533601 MS.gene93119:CDS
AGATAACAGATACAGTAAAA+GGG 0.540258 1.1:-80532948 MS.gene93119:CDS
GTGTATGCAGTCCGAGAGCA+TGG 0.564348 1.1:-80533161 MS.gene93119:intron
CTGATTACATACCATGCTCT+CGG 0.564630 1.1:+80533150 None:intergenic
ATGTTTGGGAGAAAGGGTGA+TGG 0.565214 1.1:-80533188 MS.gene93119:CDS
TCCATTACAAAATCAAAGCA+AGG 0.583726 1.1:-80533530 MS.gene93119:intron
TTCCATTAAGTAGCTCATGA+AGG 0.584791 1.1:+80533630 None:intergenic
GAAGTCATCAAAGAGATACT+TGG 0.585940 1.1:-80533579 MS.gene93119:CDS
ACCACATTAGGTCCTATAGC+TGG 0.588201 1.1:-80532808 MS.gene93119:CDS
CCAGAAAGCAACTTTGTGAG+TGG 0.608051 1.1:+80532771 None:intergenic
CTTTGTGAGTGGATATGAAG+AGG 0.608902 1.1:+80532782 None:intergenic
AAAGGGAAATTAAGGTTGGG+AGG 0.624997 1.1:-80532931 MS.gene93119:CDS
GCCAGCTATAGGACCTAATG+TGG 0.654715 1.1:+80532807 None:intergenic
TGTGAGTGGATATGAAGAGG+AGG 0.670894 1.1:+80532785 None:intergenic
CTTCCCAACTGAATGAGTTG+AGG 0.692685 1.1:+80533598 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATAATTTAATTCATCA+TGG - chr1.1:80532881-80532900 MS.gene93119:CDS 10.0%
!! TTGTTGCAACAAAAAAAAAA+TGG + chr1.1:80532779-80532798 None:intergenic 20.0%
!!! AAATACAGTTTTGTTAATGT+AGG + chr1.1:80532648-80532667 None:intergenic 20.0%
!!! AATGAAGTTTTAACTGATAA+TGG - chr1.1:80533130-80533149 MS.gene93119:intron 20.0%
!!! ATGGTCATTTTTATATTTTC+AGG - chr1.1:80532900-80532919 MS.gene93119:CDS 20.0%
!!! CTTAATTTGTTTACTATTTC+AGG - chr1.1:80533519-80533538 MS.gene93119:intron 20.0%
! AAGATAACAGATACAGTAAA+AGG - chr1.1:80533193-80533212 MS.gene93119:CDS 25.0%
! AAGTGTGAAGAAAAGATAAA+AGG + chr1.1:80533502-80533521 None:intergenic 25.0%
! AGATAACAGATACAGTAAAA+GGG - chr1.1:80533194-80533213 MS.gene93119:CDS 25.0%
! ATACAGTAAAAGGGAAATTA+AGG - chr1.1:80533203-80533222 MS.gene93119:CDS 25.0%
! ATCAATGAAAAATTGAAGGT+TGG + chr1.1:80533031-80533050 None:intergenic 25.0%
! ATTGATTAGAGTTCTCTATA+AGG - chr1.1:80533432-80533451 MS.gene93119:intron 25.0%
! TGGAATCAATGAAAAATTGA+AGG + chr1.1:80533035-80533054 None:intergenic 25.0%
! TTTACTGTATCTGTTATCTT+TGG + chr1.1:80533193-80533212 None:intergenic 25.0%
!! TATACTTTGAGTTTGAACTT+TGG - chr1.1:80533091-80533110 MS.gene93119:intron 25.0%
!!! TTTCTATGTACTTTTTCTGA+AGG + chr1.1:80533601-80533620 None:intergenic 25.0%
AAACTACTATACAATGCTCT+TGG + chr1.1:80533055-80533074 None:intergenic 30.0%
AAGTATACATCATAGCAGTT+TGG + chr1.1:80533078-80533097 None:intergenic 30.0%
AGTAAAAGGGAAATTAAGGT+TGG - chr1.1:80533207-80533226 MS.gene93119:CDS 30.0%
CAATCAAAATAACTGATGTC+CGG - chr1.1:80532733-80532752 MS.gene93119:CDS 30.0%
GTAAAAGGGAAATTAAGGTT+GGG - chr1.1:80533208-80533227 MS.gene93119:CDS 30.0%
TACAAAATCAAAGCAAGGTA+TGG - chr1.1:80532617-80532636 MS.gene93119:intron 30.0%
TCCATTACAAAATCAAAGCA+AGG - chr1.1:80532612-80532631 MS.gene93119:intron 30.0%
TGACTAAGATGAACATGTTT+GGG - chr1.1:80532940-80532959 MS.gene93119:CDS 30.0%
! ACTCTAATCAATTCTCCTTT+TGG + chr1.1:80533424-80533443 None:intergenic 30.0%
! TGTTACTTATCAACCACATT+AGG - chr1.1:80533322-80533341 MS.gene93119:intron 30.0%
!! ACCTTGCTTTGATTTTGTAA+TGG + chr1.1:80532616-80532635 None:intergenic 30.0%
AACTTCCCAACAAAATGTCT+CGG + chr1.1:80532825-80532844 None:intergenic 35.0%
ATAGAAATAGTCAGCGTAGA+TGG - chr1.1:80533614-80533633 MS.gene93119:CDS 35.0%
ATGAACATGTTTGGGAGAAA+GGG - chr1.1:80532948-80532967 MS.gene93119:CDS 35.0%
CTGACTAAGATGAACATGTT+TGG - chr1.1:80532939-80532958 MS.gene93119:CDS 35.0%
GAAGTCATCAAAGAGATACT+TGG - chr1.1:80532563-80532582 MS.gene93119:CDS 35.0%
GGAGGCAAAATTATTCAAGT+TGG - chr1.1:80533229-80533248 MS.gene93119:intron 35.0%
GTTGTATATGCCAGAAGAAT+CGG + chr1.1:80532589-80532608 None:intergenic 35.0%
TTCCATTAAGTAGCTCATGA+AGG + chr1.1:80532515-80532534 None:intergenic 35.0%
TTCCTTCATGAGCTACTTAA+TGG - chr1.1:80532510-80532529 MS.gene93119:CDS 35.0%
! ACATGCTGTATCTTTTCATG+TGG + chr1.1:80533553-80533572 None:intergenic 35.0%
! TTCATTCGGGTTAAATAGCA+TGG + chr1.1:80532679-80532698 None:intergenic 35.0%
!! AAGGAATGAGAGCATTTACA+TGG + chr1.1:80533483-80533502 None:intergenic 35.0%
!! GGCAAAATTATTCAAGTTGG+AGG - chr1.1:80533232-80533251 MS.gene93119:intron 35.0%
!!! CGGACATCAGTTATTTTGAT+TGG + chr1.1:80532735-80532754 None:intergenic 35.0%
!!! GCTTCTTCACATTTTGACTT+TGG + chr1.1:80533579-80533598 None:intergenic 35.0%
AAAGGGAAATTAAGGTTGGG+AGG - chr1.1:80533211-80533230 MS.gene93119:CDS 40.0%
ACTTCCCAACAAAATGTCTC+GGG + chr1.1:80532824-80532843 None:intergenic 40.0%
AGTTTGAACTTTGGCGTGAT+TGG - chr1.1:80533100-80533119 MS.gene93119:intron 40.0%
ATCAAGATCTCGTCACCAAA+AGG - chr1.1:80533406-80533425 MS.gene93119:intron 40.0%
ATCACCTCAACTCATTCAGT+TGG - chr1.1:80532540-80532559 MS.gene93119:CDS 40.0%
CTGATTACATACCATGCTCT+CGG + chr1.1:80532995-80533014 None:intergenic 40.0%
CTTTGTGAGTGGATATGAAG+AGG + chr1.1:80533363-80533382 None:intergenic 40.0%
GATGAACATGTTTGGGAGAA+AGG - chr1.1:80532947-80532966 MS.gene93119:CDS 40.0%
TAACTGATGTCCGGAATTCA+CGG - chr1.1:80532742-80532761 MS.gene93119:CDS 40.0%
TCACCTCAACTCATTCAGTT+GGG - chr1.1:80532541-80532560 MS.gene93119:CDS 40.0%
! AACCTCGGGATGTTTTCATT+CGG + chr1.1:80532693-80532712 None:intergenic 40.0%
! AACTTTTCAGCGTACAAGAC+GGG - chr1.1:80533275-80533294 MS.gene93119:intron 40.0%
! ATGTCCCGAGACATTTTGTT+GGG - chr1.1:80532817-80532836 MS.gene93119:CDS 40.0%
! CAACTTTTCAGCGTACAAGA+CGG - chr1.1:80533274-80533293 MS.gene93119:intron 40.0%
! TATGTCCCGAGACATTTTGT+TGG - chr1.1:80532816-80532835 MS.gene93119:CDS 40.0%
!!! GGTTTCGACTTCTGGTTTTT+GGG - chr1.1:80533635-80533654 MS.gene93119:CDS 40.0%
!!! TGGTTTCGACTTCTGGTTTT+TGG - chr1.1:80533634-80533653 MS.gene93119:CDS 40.0%
!!! TTTTGGTGACGAGATCTTGA+TGG + chr1.1:80533407-80533426 None:intergenic 40.0%
ACCACATTAGGTCCTATAGC+TGG - chr1.1:80533334-80533353 MS.gene93119:intron 45.0%
ATGTTTGGGAGAAAGGGTGA+TGG - chr1.1:80532954-80532973 MS.gene93119:CDS 45.0%
CCAGAAAGCAACTTTGTGAG+TGG + chr1.1:80533374-80533393 None:intergenic 45.0%
CTTCCCAACTGAATGAGTTG+AGG + chr1.1:80532547-80532566 None:intergenic 45.0%
TGTGAGTGGATATGAAGAGG+AGG + chr1.1:80533360-80533379 None:intergenic 45.0%
! ACCTCGGGATGTTTTCATTC+GGG + chr1.1:80532692-80532711 None:intergenic 45.0%
! ACTTTTCAGCGTACAAGACG+GGG - chr1.1:80533276-80533295 MS.gene93119:intron 45.0%
! AGATACTTGGCCGATTCTTC+TGG - chr1.1:80532576-80532595 MS.gene93119:CDS 45.0%
! CCACTCACAAAGTTGCTTTC+TGG - chr1.1:80533371-80533390 MS.gene93119:intron 45.0%
!! TGATTGGCTGCTGCTTTGTT+CGG + chr1.1:80532719-80532738 None:intergenic 45.0%
!! TGAATTAAATTATATTAAAT+TGG + chr1.1:80532878-80532897 None:intergenic 5.0%
ACCACTACCTCCGTGAATTC+CGG + chr1.1:80532755-80532774 None:intergenic 50.0%
ACCCGAATGAAAACATCCCG+AGG - chr1.1:80532688-80532707 MS.gene93119:intron 50.0%
ATGAAGAGGAGGCCAGCTAT+AGG + chr1.1:80533349-80533368 None:intergenic 50.0%
CTGTAACCTGCAGTGAGACT+TGG - chr1.1:80533166-80533185 MS.gene93119:CDS 50.0%
GCCAGCTATAGGACCTAATG+TGG + chr1.1:80533338-80533357 None:intergenic 50.0%
GCGTAGATGGTTTCGACTTC+TGG - chr1.1:80533627-80533646 MS.gene93119:CDS 50.0%
GTCTCGGGACATATTCACGA+TGG + chr1.1:80532809-80532828 None:intergenic 50.0%
TTTGGTCCAAGTCTCACTGC+AGG + chr1.1:80533175-80533194 None:intergenic 50.0%
! TGCTTTGTTCGGGACAACCT+CGG + chr1.1:80532708-80532727 None:intergenic 50.0%
!! GATTGGCTGCTGCTTTGTTC+GGG + chr1.1:80532718-80532737 None:intergenic 50.0%
CTGATGTCCGGAATTCACGG+AGG - chr1.1:80532745-80532764 MS.gene93119:CDS 55.0%
GTGTATGCAGTCCGAGAGCA+TGG - chr1.1:80532981-80533000 MS.gene93119:intron 55.0%
TCCGGAATTCACGGAGGTAG+TGG - chr1.1:80532751-80532770 MS.gene93119:CDS 55.0%
! GCTTTGTTCGGGACAACCTC+GGG + chr1.1:80532707-80532726 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 80532498 80533666 80532498 ID=MS.gene93119
chr1.1 mRNA 80532498 80533666 80532498 ID=MS.gene93119.t1;Parent=MS.gene93119
chr1.1 exon 80533531 80533666 80533531 ID=MS.gene93119.t1.exon1;Parent=MS.gene93119.t1
chr1.1 CDS 80533531 80533666 80533531 ID=cds.MS.gene93119.t1;Parent=MS.gene93119.t1
chr1.1 exon 80533162 80533242 80533162 ID=MS.gene93119.t1.exon2;Parent=MS.gene93119.t1
chr1.1 CDS 80533162 80533242 80533162 ID=cds.MS.gene93119.t1;Parent=MS.gene93119.t1
chr1.1 exon 80532711 80532985 80532711 ID=MS.gene93119.t1.exon3;Parent=MS.gene93119.t1
chr1.1 CDS 80532711 80532985 80532711 ID=cds.MS.gene93119.t1;Parent=MS.gene93119.t1
chr1.1 exon 80532498 80532623 80532498 ID=MS.gene93119.t1.exon4;Parent=MS.gene93119.t1
chr1.1 CDS 80532498 80532623 80532498 ID=cds.MS.gene93119.t1;Parent=MS.gene93119.t1
Gene Sequence

>MS.gene93119

ATGAAGACGTCATTCCTTCATGAGCTACTTAATGGAATTTCTATCACCTCAACTCATTCAGTTGGGAAGTCATCAAAGAGATACTTGGCCGATTCTTCTGGCATATACAACAAATCCATTACAAAATCAAAGCAAGGTATGGACAAACCTACATTAACAAAACTGTATTTCATAAGAGCCATGCTATTTAACCCGAATGAAAACATCCCGAGGTTGTCCCGAACAAAGCAGCAGCCAATCAAAATAACTGATGTCCGGAATTCACGGAGGTAGTGGTGCCATTTTTTTTTTGTTGCAACAAGCAATTTCCATCGTGAATATGTCCCGAGACATTTTGTTGGGAAGTTTAGCAATTTTCATTTCATAAATACTTCATTCCAATTTAATATAATTTAATTCATCATGGTCATTTTTATATTTTCAGGTAAATTTAATTCAGTTCTGACTAAGATGAACATGTTTGGGAGAAAGGGTGATGGTTTTGTGTATGCAGTCCGAGAGCATGGTATGTAATCAGTAATCTTTAAACACCAACCTTCAATTTTTCATTGATTCCAAGAGCATTGTATAGTAGTTTCCAAACTGCTATGATGTATACTTTGAGTTTGAACTTTGGCGTGATTGGAAAATTGAATGAAGTTTTAACTGATAATGGCTTAAAACTGTTACTGTAACCTGCAGTGAGACTTGGACCAAAGATAACAGATACAGTAAAAGGGAAATTAAGGTTGGGAGGCAAAATTATTCAAGTTGGAGGAATAGAGAAAGTGTTTATGCAACTTTTCAGCGTACAAGACGGGGAGAAGCTGTTGAAAGCGTCACAATGTTACTTATCAACCACATTAGGTCCTATAGCTGGCCTCCTCTTCATATCCACTCACAAAGTTGCTTTCTGGAGTGACAAATCCATCAAGATCTCGTCACCAAAAGGAGAATTGATTAGAGTTCTCTATAAGGTGATTTTCTTACTTTTTTATAATGTCCATGTAAATGCTCTCATTCCTTTTATCTTTTCTTCACACTTAATTTGTTTACTATTTCAGGTCTCTATTCCACATGAAAAGATACAGCATGTCAACCAAAGTCAAAATGTGAAGAAGCCTTCAGAAAAAGTACATAGAAATAGTCAGCGTAGATGGTTTCGACTTCTGGTTTTTGGGTTTCTTTAA

Protein sequence

>MS.gene93119.t1

MKTSFLHELLNGISITSTHSVGKSSKRYLADSSGIYNKSITKSKQGKFNSVLTKMNMFGRKGDGFVYAVREHVRLGPKITDTVKGKLRLGGKIIQVGGIEKVFMQLFSVQDGEKLLKASQCYLSTTLGPIAGLLFISTHKVAFWSDKSIKISSPKGELIRVLYKVSIPHEKIQHVNQSQNVKKPSEKVHRNSQRRWFRLLVFGFL