Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038370.t1 | XP_003617458.1 | 98.1 | 157 | 3 | 0 | 1 | 157 | 1 | 157 | 7.60E-84 | 319.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038370.t1 | Q682H0 | 63.9 | 158 | 56 | 1 | 1 | 157 | 1 | 158 | 1.6e-53 | 210.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038370.t1 | B7FNC9 | 98.1 | 157 | 3 | 0 | 1 | 157 | 1 | 157 | 5.5e-84 | 319.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene91904 | MS.gene038370 | PPI |
| MS.gene038370 | MS.gene074024 | PPI |
| MS.gene038370 | MS.gene78507 | PPI |
| MS.gene038370 | MS.gene032837 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038370.t1 | MTR_5g091800 | 98.089 | 157 | 3 | 0 | 1 | 157 | 1 | 157 | 2.93e-115 | 323 |
| MS.gene038370.t1 | MTR_3g452470 | 78.481 | 158 | 33 | 1 | 1 | 157 | 1 | 158 | 8.81e-94 | 268 |
| MS.gene038370.t1 | MTR_4g108350 | 51.333 | 150 | 71 | 1 | 4 | 153 | 12 | 159 | 2.38e-46 | 149 |
| MS.gene038370.t1 | MTR_5g039530 | 44.578 | 166 | 74 | 2 | 2 | 155 | 7 | 166 | 1.18e-40 | 135 |
| MS.gene038370.t1 | MTR_4g108350 | 48.000 | 150 | 67 | 2 | 4 | 153 | 12 | 150 | 3.87e-39 | 130 |
| MS.gene038370.t1 | MTR_3g099010 | 48.760 | 121 | 56 | 1 | 1 | 121 | 1 | 115 | 9.50e-34 | 116 |
| MS.gene038370.t1 | MTR_8g096670 | 50.962 | 104 | 51 | 0 | 1 | 104 | 1 | 104 | 1.21e-32 | 112 |
| MS.gene038370.t1 | MTR_8g096670 | 55.682 | 88 | 39 | 0 | 17 | 104 | 7 | 94 | 7.77e-30 | 104 |
| MS.gene038370.t1 | MTR_4g093260 | 31.333 | 150 | 89 | 2 | 3 | 147 | 6 | 146 | 2.50e-20 | 87.0 |
| MS.gene038370.t1 | MTR_4g093510 | 33.088 | 136 | 77 | 2 | 17 | 147 | 20 | 146 | 3.59e-20 | 86.3 |
| MS.gene038370.t1 | MTR_4g093270 | 31.159 | 138 | 81 | 2 | 17 | 149 | 17 | 145 | 1.08e-19 | 84.0 |
| MS.gene038370.t1 | MTR_4g093260 | 32.540 | 126 | 71 | 2 | 27 | 147 | 21 | 137 | 5.28e-17 | 77.4 |
| MS.gene038370.t1 | MTR_4g093280 | 30.496 | 141 | 84 | 2 | 17 | 152 | 20 | 151 | 1.93e-14 | 69.7 |
| MS.gene038370.t1 | MTR_5g019720 | 29.688 | 128 | 78 | 2 | 7 | 134 | 28 | 143 | 3.64e-14 | 67.0 |
| MS.gene038370.t1 | MTR_5g019720 | 32.990 | 97 | 61 | 1 | 7 | 103 | 28 | 120 | 3.19e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038370.t1 | AT2G42820 | 63.924 | 158 | 56 | 1 | 1 | 157 | 1 | 158 | 1.18e-74 | 220 |
| MS.gene038370.t1 | AT1G74520 | 45.395 | 152 | 81 | 1 | 4 | 155 | 9 | 158 | 1.43e-41 | 137 |
| MS.gene038370.t1 | AT1G74520 | 45.395 | 152 | 81 | 1 | 4 | 155 | 9 | 158 | 1.43e-41 | 137 |
| MS.gene038370.t1 | AT1G69700 | 44.295 | 149 | 80 | 1 | 2 | 147 | 9 | 157 | 1.68e-36 | 124 |
| MS.gene038370.t1 | AT5G50720 | 58.095 | 105 | 44 | 0 | 1 | 105 | 1 | 105 | 2.82e-35 | 119 |
| MS.gene038370.t1 | AT5G62490 | 56.842 | 95 | 41 | 0 | 7 | 101 | 12 | 106 | 2.17e-34 | 118 |
| MS.gene038370.t1 | AT4G24960 | 45.669 | 127 | 68 | 1 | 8 | 134 | 8 | 133 | 3.92e-32 | 112 |
| MS.gene038370.t1 | AT4G24960 | 40.441 | 136 | 71 | 1 | 20 | 155 | 1 | 126 | 1.28e-29 | 105 |
| MS.gene038370.t1 | AT4G24960 | 41.748 | 103 | 59 | 1 | 32 | 134 | 1 | 102 | 7.56e-23 | 87.4 |
| MS.gene038370.t1 | AT4G36720 | 36.842 | 95 | 56 | 1 | 7 | 101 | 36 | 126 | 5.19e-16 | 72.0 |
Find 34 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCAACAGTGGCTAACATAT+TGG | 0.311228 | 5.4:+72739529 | MS.gene038370:CDS |
| GAACGTTATATTGATAGATT+TGG | 0.323087 | 5.4:+72739902 | MS.gene038370:CDS |
| CCAACAGTGGCTAACATATT+GGG | 0.341811 | 5.4:+72739530 | MS.gene038370:CDS |
| CAACTTACAAGGTCCTATAT+TGG | 0.344124 | 5.4:+72739586 | MS.gene038370:CDS |
| ATCACTCTCTCAAATGCATC+TGG | 0.360770 | 5.4:-72739926 | None:intergenic |
| ATATCAATAGGTTTCCCATT+TGG | 0.373951 | 5.4:+72739696 | MS.gene038370:intron |
| AGACATTTGGACACAACAAT+AGG | 0.378599 | 5.4:+72738981 | MS.gene038370:CDS |
| ATTGCTGAACCTACCAATAT+AGG | 0.380907 | 5.4:-72739599 | None:intergenic |
| TGGAGCAATACTAAGACATT+TGG | 0.388072 | 5.4:+72738968 | MS.gene038370:CDS |
| AAGCTTCAAGTATGGCCAAA+TGG | 0.413601 | 5.4:-72739711 | None:intergenic |
| CTTCGAGCTTTCAACTTACA+AGG | 0.426910 | 5.4:+72739575 | MS.gene038370:CDS |
| AAAATCATCATGGGTGTCCT+TGG | 0.439409 | 5.4:+72738948 | None:intergenic |
| AGCTTCAAGTATGGCCAAAT+GGG | 0.446743 | 5.4:-72739710 | None:intergenic |
| CAGAACACAAGCTTCAAGTA+TGG | 0.447263 | 5.4:-72739719 | None:intergenic |
| GACATTTGGACACAACAATA+GGG | 0.477953 | 5.4:+72738982 | MS.gene038370:CDS |
| TGAAGCTTGTGTTCTGCGTA+TGG | 0.484389 | 5.4:+72739726 | MS.gene038370:CDS |
| TATTGAAAGTCCTTCAACCT+TGG | 0.507265 | 5.4:+72739503 | MS.gene038370:CDS |
| TGTTGGTCATCCAAGGTTGA+AGG | 0.521506 | 5.4:-72739513 | None:intergenic |
| AGAACCTTCTTGTACTCCTC+AGG | 0.524082 | 5.4:-72739845 | None:intergenic |
| CCCAATATGTTAGCCACTGT+TGG | 0.527231 | 5.4:-72739530 | None:intergenic |
| TAGCCACTGTTGGTCATCCA+AGG | 0.555283 | 5.4:-72739520 | None:intergenic |
| AGGATAAAGAAGCATTACTC+CGG | 0.560010 | 5.4:-72739375 | None:intergenic |
| TTGGTACTGCCAATGTTCAA+TGG | 0.561469 | 5.4:+72739749 | MS.gene038370:CDS |
| GCTTGTGTTCTGCGTATGGT+TGG | 0.576715 | 5.4:+72739730 | MS.gene038370:CDS |
| GGATAAAGAAGCATTACTCC+GGG | 0.584585 | 5.4:-72739374 | None:intergenic |
| TCATAGATACGCATCAATGA+GGG | 0.589923 | 5.4:+72739479 | MS.gene038370:intron |
| ATCATAGATACGCATCAATG+AGG | 0.601236 | 5.4:+72739478 | MS.gene038370:intron |
| TCACTCTCTCAAATGCATCT+GGG | 0.601880 | 5.4:-72739925 | None:intergenic |
| TAAGCTGCTCCATTGAACAT+TGG | 0.632735 | 5.4:-72739758 | None:intergenic |
| TTATCCTGAGGAGTACAAGA+AGG | 0.638050 | 5.4:+72739841 | MS.gene038370:CDS |
| AATGTCTTAGTATTGCTCCA+AGG | 0.660944 | 5.4:-72738965 | None:intergenic |
| ACATATATGAGAATTATGCA+AGG | 0.691799 | 5.4:+72739780 | MS.gene038370:CDS |
| CAACCTTGGATGACCAACAG+TGG | 0.691907 | 5.4:+72739517 | MS.gene038370:CDS |
| TGGAAATTACAATTATCCTG+AGG | 0.710511 | 5.4:+72739829 | MS.gene038370:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTAATAAAAAAATATCAAT+AGG | + | chr5.4:72739684-72739703 | MS.gene038370:intron | 10.0% |
| !! | TAAAGATATATTTATAACTT+TGG | - | chr5.4:72739982-72740001 | None:intergenic | 10.0% |
| !!! | TAATTATCTTATCTTTTTAT+TGG | + | chr5.4:72739117-72739136 | MS.gene038370:intron | 10.0% |
| !! | ATAAGATAATTATAGAAAAG+TGG | - | chr5.4:72739109-72739128 | None:intergenic | 15.0% |
| !!! | ACAATTTGATAATATTTATC+TGG | + | chr5.4:72739661-72739680 | MS.gene038370:intron | 15.0% |
| !! | AATATGATCATTACTTACAA+AGG | - | chr5.4:72739398-72739417 | None:intergenic | 20.0% |
| !! | TCTATTTCACTTTATATTTC+AGG | + | chr5.4:72740046-72740065 | MS.gene038370:intron | 20.0% |
| !! | TTCTATAATTATCTAGATGA+AGG | + | chr5.4:72739219-72739238 | MS.gene038370:intron | 20.0% |
| !!! | TTATCTTATCTTTTTATTGG+CGG | + | chr5.4:72739120-72739139 | MS.gene038370:intron | 20.0% |
| ! | AAAAGTAGGATGTTATTAAG+AGG | - | chr5.4:72739342-72739361 | None:intergenic | 25.0% |
| ! | ACATATATGAGAATTATGCA+AGG | + | chr5.4:72739780-72739799 | MS.gene038370:CDS | 25.0% |
| ! | GAAATTCTTTCAAAACACAT+GGG | + | chr5.4:72739280-72739299 | MS.gene038370:intron | 25.0% |
| ! | GAACGTTATATTGATAGATT+TGG | + | chr5.4:72739902-72739921 | MS.gene038370:CDS | 25.0% |
| !! | GTCAAAAAATTTGGTAACTA+TGG | + | chr5.4:72739809-72739828 | MS.gene038370:CDS | 25.0% |
| AGGACATACGTCAAAAAATT+TGG | + | chr5.4:72739800-72739819 | MS.gene038370:CDS | 30.0% | |
| ATATCAATAGGTTTCCCATT+TGG | + | chr5.4:72739696-72739715 | MS.gene038370:intron | 30.0% | |
| CGAAATTCTTTCAAAACACA+TGG | + | chr5.4:72739279-72739298 | MS.gene038370:intron | 30.0% | |
| GACAATGCATAAAATATGCA+AGG | - | chr5.4:72739168-72739187 | None:intergenic | 30.0% | |
| TGGAAATTACAATTATCCTG+AGG | + | chr5.4:72739829-72739848 | MS.gene038370:CDS | 30.0% | |
| !! | TCGTACATGCAATATTTTGA+AGG | - | chr5.4:72739262-72739281 | None:intergenic | 30.0% |
| AATGTCTTAGTATTGCTCCA+AGG | - | chr5.4:72738968-72738987 | None:intergenic | 35.0% | |
| AGACATTTGGACACAACAAT+AGG | + | chr5.4:72738981-72739000 | MS.gene038370:CDS | 35.0% | |
| ATCATAGATACGCATCAATG+AGG | + | chr5.4:72739478-72739497 | MS.gene038370:intron | 35.0% | |
| ATGGGACACACTCTAAATTT+AGG | + | chr5.4:72739298-72739317 | MS.gene038370:intron | 35.0% | |
| ATTGCTGAACCTACCAATAT+AGG | - | chr5.4:72739602-72739621 | None:intergenic | 35.0% | |
| CAACTTACAAGGTCCTATAT+TGG | + | chr5.4:72739586-72739605 | MS.gene038370:CDS | 35.0% | |
| GACATTTGGACACAACAATA+GGG | + | chr5.4:72738982-72739001 | MS.gene038370:CDS | 35.0% | |
| TATTGAAAGTCCTTCAACCT+TGG | + | chr5.4:72739503-72739522 | MS.gene038370:CDS | 35.0% | |
| TCATAGATACGCATCAATGA+GGG | + | chr5.4:72739479-72739498 | MS.gene038370:intron | 35.0% | |
| TGCATAAAATATGCAAGGTC+CGG | - | chr5.4:72739163-72739182 | None:intergenic | 35.0% | |
| TGGAGCAATACTAAGACATT+TGG | + | chr5.4:72738968-72738987 | MS.gene038370:CDS | 35.0% | |
| TTACAAGGTCCTATATTGGT+AGG | + | chr5.4:72739590-72739609 | MS.gene038370:intron | 35.0% | |
| ! | TCTTATCTTTTTATTGGCGG+TGG | + | chr5.4:72739123-72739142 | MS.gene038370:intron | 35.0% |
| !! | AGGATAAAGAAGCATTACTC+CGG | - | chr5.4:72739378-72739397 | None:intergenic | 35.0% |
| !!! | ATCCTACTTTTTTGTGTACC+AGG | + | chr5.4:72739351-72739370 | MS.gene038370:intron | 35.0% |
| AAGCTTCAAGTATGGCCAAA+TGG | - | chr5.4:72739714-72739733 | None:intergenic | 40.0% | |
| ACCAACAGTGGCTAACATAT+TGG | + | chr5.4:72739529-72739548 | MS.gene038370:CDS | 40.0% | |
| AGCTTCAAGTATGGCCAAAT+GGG | - | chr5.4:72739713-72739732 | None:intergenic | 40.0% | |
| ATCACTCTCTCAAATGCATC+TGG | - | chr5.4:72739929-72739948 | None:intergenic | 40.0% | |
| CAGAACACAAGCTTCAAGTA+TGG | - | chr5.4:72739722-72739741 | None:intergenic | 40.0% | |
| CATAAAATATGCAAGGTCCG+GGG | - | chr5.4:72739161-72739180 | None:intergenic | 40.0% | |
| CCAACAGTGGCTAACATATT+GGG | + | chr5.4:72739530-72739549 | MS.gene038370:CDS | 40.0% | |
| CTTCGAGCTTTCAACTTACA+AGG | + | chr5.4:72739575-72739594 | MS.gene038370:CDS | 40.0% | |
| GCATAAAATATGCAAGGTCC+GGG | - | chr5.4:72739162-72739181 | None:intergenic | 40.0% | |
| TAAGCTGCTCCATTGAACAT+TGG | - | chr5.4:72739761-72739780 | None:intergenic | 40.0% | |
| TCACTCTCTCAAATGCATCT+GGG | - | chr5.4:72739928-72739947 | None:intergenic | 40.0% | |
| TTGGTACTGCCAATGTTCAA+TGG | + | chr5.4:72739749-72739768 | MS.gene038370:CDS | 40.0% | |
| ! | TTATCCTGAGGAGTACAAGA+AGG | + | chr5.4:72739841-72739860 | MS.gene038370:CDS | 40.0% |
| !! | GGATAAAGAAGCATTACTCC+GGG | - | chr5.4:72739377-72739396 | None:intergenic | 40.0% |
| AGAACCTTCTTGTACTCCTC+AGG | - | chr5.4:72739848-72739867 | None:intergenic | 45.0% | |
| CCCAATATGTTAGCCACTGT+TGG | - | chr5.4:72739533-72739552 | None:intergenic | 45.0% | |
| GGCCTGGTACACAAAAAAGT+AGG | - | chr5.4:72739356-72739375 | None:intergenic | 45.0% | |
| TGAAGCTTGTGTTCTGCGTA+TGG | + | chr5.4:72739726-72739745 | MS.gene038370:CDS | 45.0% | |
| TGTTGGTCATCCAAGGTTGA+AGG | - | chr5.4:72739516-72739535 | None:intergenic | 45.0% | |
| ! | ATCTTTTTATTGGCGGTGGC+CGG | + | chr5.4:72739127-72739146 | MS.gene038370:intron | 45.0% |
| ! | TGAGCTTAGCTCAGTTGGTA+TGG | - | chr5.4:72739190-72739209 | None:intergenic | 45.0% |
| !!! | ACTTTTTTGTGTACCAGGCC+CGG | + | chr5.4:72739356-72739375 | MS.gene038370:intron | 45.0% |
| CAACCTTGGATGACCAACAG+TGG | + | chr5.4:72739517-72739536 | MS.gene038370:CDS | 50.0% | |
| GCTTGTGTTCTGCGTATGGT+TGG | + | chr5.4:72739730-72739749 | MS.gene038370:CDS | 50.0% | |
| TAGCCACTGTTGGTCATCCA+AGG | - | chr5.4:72739523-72739542 | None:intergenic | 50.0% | |
| ! | TCTTTTTATTGGCGGTGGCC+GGG | + | chr5.4:72739128-72739147 | MS.gene038370:intron | 50.0% |
| CCAACTGAGCTAAGCTCACG+TGG | + | chr5.4:72739192-72739211 | MS.gene038370:intron | 55.0% | |
| CCACGTGAGCTTAGCTCAGT+TGG | - | chr5.4:72739195-72739214 | None:intergenic | 55.0% | |
| !! | AAGAAGCATTACTCCGGGCC+TGG | - | chr5.4:72739372-72739391 | None:intergenic | 55.0% |
| AAGGTCCGGGGTTCGAATCC+CGG | - | chr5.4:72739149-72739168 | None:intergenic | 60.0% | |
| GGTGGCCGGGATTCGAACCC+CGG | + | chr5.4:72739141-72739160 | MS.gene038370:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 72738957 | 72740094 | 72738957 | ID=MS.gene038370 |
| chr5.4 | mRNA | 72738957 | 72740094 | 72738957 | ID=MS.gene038370.t1;Parent=MS.gene038370 |
| chr5.4 | exon | 72738957 | 72739003 | 72738957 | ID=MS.gene038370.t1.exon1;Parent=MS.gene038370.t1 |
| chr5.4 | CDS | 72738957 | 72739003 | 72738957 | ID=cds.MS.gene038370.t1;Parent=MS.gene038370.t1 |
| chr5.4 | exon | 72739373 | 72739399 | 72739373 | ID=MS.gene038370.t1.exon2;Parent=MS.gene038370.t1 |
| chr5.4 | CDS | 72739373 | 72739399 | 72739373 | ID=cds.MS.gene038370.t1;Parent=MS.gene038370.t1 |
| chr5.4 | exon | 72739485 | 72739607 | 72739485 | ID=MS.gene038370.t1.exon3;Parent=MS.gene038370.t1 |
| chr5.4 | CDS | 72739485 | 72739607 | 72739485 | ID=cds.MS.gene038370.t1;Parent=MS.gene038370.t1 |
| chr5.4 | exon | 72739706 | 72739955 | 72739706 | ID=MS.gene038370.t1.exon4;Parent=MS.gene038370.t1 |
| chr5.4 | CDS | 72739706 | 72739955 | 72739706 | ID=cds.MS.gene038370.t1;Parent=MS.gene038370.t1 |
| chr5.4 | exon | 72740068 | 72740094 | 72740068 | ID=MS.gene038370.t1.exon5;Parent=MS.gene038370.t1 |
| chr5.4 | CDS | 72740068 | 72740094 | 72740068 | ID=cds.MS.gene038370.t1;Parent=MS.gene038370.t1 |
| Gene Sequence |
| Protein sequence |
