AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene040643

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040643.t1 MTR_1g050448 97.867 375 8 0 1 375 1 375 0.0 772
MS.gene040643.t1 MTR_1g112210 75.069 361 85 3 19 375 23 382 0.0 580
MS.gene040643.t1 MTR_1g112210 72.853 361 80 4 19 375 23 369 0.0 551
MS.gene040643.t1 MTR_5g071350 39.316 351 190 5 45 375 52 399 1.00e-96 294
MS.gene040643.t1 MTR_2g015720 42.246 374 188 9 9 375 6 358 1.99e-95 289
MS.gene040643.t1 MTR_2g091175 42.560 336 173 7 47 375 44 366 4.59e-93 283
MS.gene040643.t1 MTR_7g093450 41.449 345 185 6 48 375 243 587 6.79e-93 290
MS.gene040643.t1 MTR_2g073510 41.791 335 177 5 49 375 29 353 3.06e-91 278
MS.gene040643.t1 MTR_0136s0010 40.870 345 187 6 48 375 165 509 4.73e-91 283
MS.gene040643.t1 MTR_3g049330 39.572 374 192 8 10 375 3 350 5.82e-91 277
MS.gene040643.t1 MTR_2g073500 43.413 334 161 9 53 375 29 345 2.48e-89 273
MS.gene040643.t1 MTR_3g464570 38.988 336 190 5 53 375 43 376 4.18e-88 271
MS.gene040643.t1 MTR_4g079700 38.933 375 196 10 14 375 9 363 1.97e-87 269
MS.gene040643.t1 MTR_4g088030 39.521 334 185 5 48 375 28 350 9.91e-87 266
MS.gene040643.t1 MTR_5g034810 39.093 353 194 8 31 375 13 352 1.25e-85 264
MS.gene040643.t1 MTR_4g126950 34.908 381 221 6 21 375 67 446 5.72e-85 265
MS.gene040643.t1 MTR_5g059400 35.770 383 222 7 17 375 133 515 2.05e-84 266
MS.gene040643.t1 MTR_1g116130 35.770 383 222 7 17 375 133 515 2.05e-84 266
MS.gene040643.t1 MTR_4g087730 40.000 335 184 5 47 375 39 362 2.89e-84 260
MS.gene040643.t1 MTR_4g016940 39.244 344 190 7 51 375 84 427 2.90e-84 263
MS.gene040643.t1 MTR_3g021090 39.823 339 182 6 40 371 21 344 1.67e-83 260
MS.gene040643.t1 MTR_5g033800 37.830 341 196 5 51 375 143 483 2.02e-83 262
MS.gene040643.t1 MTR_7g109550 37.425 334 187 6 53 371 137 463 2.34e-83 261
MS.gene040643.t1 MTR_3g035830 39.589 341 184 6 40 373 21 346 3.31e-82 254
MS.gene040643.t1 MTR_4g121560 37.135 342 194 6 53 375 114 453 2.50e-81 256
MS.gene040643.t1 MTR_8g463790 39.942 343 182 8 53 375 71 409 4.46e-81 254
MS.gene040643.t1 MTR_4g014440 37.037 351 198 9 48 375 57 407 1.74e-80 252
MS.gene040643.t1 MTR_3g021060 38.554 332 185 5 49 373 42 361 2.29e-80 251
MS.gene040643.t1 MTR_1g058380 36.476 403 219 11 1 375 1 394 5.23e-79 248
MS.gene040643.t1 MTR_8g069790 37.647 340 196 5 52 375 177 516 2.25e-78 250
MS.gene040643.t1 MTR_1g111010 39.881 336 169 11 50 375 29 341 2.41e-78 245
MS.gene040643.t1 MTR_7g070060 34.336 399 226 11 10 375 14 409 3.35e-77 244
MS.gene040643.t1 MTR_7g068920 38.841 345 192 7 50 375 77 421 3.42e-76 241
MS.gene040643.t1 MTR_3g070900 37.353 340 192 7 53 371 106 445 5.68e-76 242
MS.gene040643.t1 MTR_5g082880 37.391 345 192 8 51 371 163 507 5.08e-73 236
MS.gene040643.t1 MTR_2g022560 35.795 352 196 7 48 375 92 437 5.25e-73 234
MS.gene040643.t1 MTR_3g071650 35.673 342 201 7 53 375 89 430 1.37e-71 230
MS.gene040643.t1 MTR_5g083260 33.417 398 235 10 8 375 27 424 2.58e-71 229
MS.gene040643.t1 MTR_7g094750 35.311 354 206 6 43 375 81 432 1.32e-70 228
MS.gene040643.t1 MTR_4g014430 35.244 349 206 8 45 375 100 446 1.76e-70 228
MS.gene040643.t1 MTR_3g464570 38.603 272 153 4 116 375 3 272 1.13e-65 210
MS.gene040643.t1 MTR_8g093530 32.853 347 207 7 44 375 81 416 1.89e-62 206
MS.gene040643.t1 MTR_3g090120 35.072 345 196 7 49 375 125 459 4.68e-62 206
MS.gene040643.t1 MTR_5g033800 37.815 238 137 3 51 277 143 380 5.85e-57 191
MS.gene040643.t1 MTR_7g070060 35.943 281 158 7 16 277 20 297 3.02e-56 186
MS.gene040643.t1 MTR_7g070060 33.106 293 175 7 104 375 15 307 5.14e-53 178
MS.gene040643.t1 MTR_7g094750 34.677 248 149 3 43 277 81 328 3.09e-50 171
MS.gene040643.t1 MTR_8g027900 27.363 402 242 12 1 375 177 555 6.99e-42 154
MS.gene040643.t1 MTR_6g061710 31.383 376 203 16 40 375 154 514 5.01e-41 151
MS.gene040643.t1 MTR_8g093870 30.295 373 216 14 35 375 112 472 1.21e-39 147
MS.gene040643.t1 MTR_1g076690 30.769 377 209 16 36 375 209 570 6.49e-39 146
MS.gene040643.t1 MTR_5g069320 28.425 292 140 6 96 368 145 386 7.99e-38 140
MS.gene040643.t1 MTR_5g035080 28.169 355 214 10 53 375 75 420 3.81e-37 139
MS.gene040643.t1 MTR_7g033230 28.000 350 225 7 51 375 262 609 4.63e-37 141
MS.gene040643.t1 MTR_7g019120 29.412 357 211 13 49 375 75 420 6.65e-37 139
MS.gene040643.t1 MTR_4g092830 27.762 353 217 12 53 375 48 392 7.80e-37 138
MS.gene040643.t1 MTR_4g125060 30.919 359 209 16 45 375 124 471 1.30e-36 138
MS.gene040643.t1 MTR_4g125060 30.919 359 209 16 45 375 163 510 1.43e-36 139
MS.gene040643.t1 MTR_7g033230 28.000 350 225 7 51 375 332 679 1.65e-36 140
MS.gene040643.t1 MTR_4g125060 30.919 359 209 16 45 375 192 539 2.32e-36 139
MS.gene040643.t1 MTR_8g104970 27.957 372 225 12 39 375 102 465 7.60e-36 136
MS.gene040643.t1 MTR_3g109840 28.045 353 214 9 53 375 73 415 1.66e-35 134
MS.gene040643.t1 MTR_5g035090 28.726 369 211 15 44 375 74 427 9.96e-35 132
MS.gene040643.t1 MTR_8g099300 26.027 365 215 12 50 375 71 419 3.74e-34 131
MS.gene040643.t1 MTR_5g035150 28.852 357 220 12 48 375 80 431 2.65e-33 129
MS.gene040643.t1 MTR_6g016875 28.492 358 224 10 45 375 56 408 2.12e-32 126
MS.gene040643.t1 MTR_6g016880 26.214 412 252 12 4 375 20 419 6.19e-32 125
MS.gene040643.t1 MTR_2g016060 28.177 362 197 13 57 375 59 400 1.52e-31 123
MS.gene040643.t1 MTR_6g016240 28.293 410 234 18 4 371 21 412 7.89e-31 122
MS.gene040643.t1 MTR_5g035070 30.055 366 212 13 44 375 78 433 2.67e-29 118
MS.gene040643.t1 MTR_6g007777 28.142 366 206 15 48 375 111 457 1.11e-28 116
MS.gene040643.t1 MTR_7g019140 44.792 96 52 1 49 143 75 170 1.84e-22 93.6
MS.gene040643.t1 MTR_5g035120 33.571 140 88 2 8 142 26 165 8.65e-20 86.3
MS.gene040643.t1 MTR_3g021150 37.037 108 62 1 238 339 1 108 8.86e-18 79.0
MS.gene040643.t1 MTR_2g022580 32.773 119 68 2 112 218 2 120 2.75e-15 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040643.t1 AT3G54260 61.842 380 134 5 1 375 1 374 9.74e-170 479
MS.gene040643.t1 AT2G31110 39.623 371 205 6 13 375 3 362 9.15e-93 282
MS.gene040643.t1 AT5G06700 40.870 345 187 6 48 375 250 594 1.59e-90 284
MS.gene040643.t1 AT5G58600 39.130 345 193 6 44 375 60 400 6.25e-90 276
MS.gene040643.t1 AT1G29050 39.714 350 185 5 41 375 40 378 6.93e-90 275
MS.gene040643.t1 AT2G30900 41.369 336 175 8 51 375 41 365 3.76e-88 271
MS.gene040643.t1 AT2G34070 38.576 337 189 5 46 375 58 383 1.26e-87 270
MS.gene040643.t1 AT2G42570 39.940 333 183 5 49 375 43 364 3.31e-87 268
MS.gene040643.t1 AT3G12060 39.710 345 191 5 48 375 191 535 3.70e-87 274
MS.gene040643.t1 AT5G20590 38.551 345 194 6 48 375 136 479 5.46e-85 266
MS.gene040643.t1 AT5G01360 38.728 346 190 8 51 375 34 378 1.43e-84 262
MS.gene040643.t1 AT5G01360 38.728 346 190 8 51 375 90 434 5.28e-84 262
MS.gene040643.t1 AT5G49340 38.122 362 203 8 35 375 80 441 1.51e-83 262
MS.gene040643.t1 AT3G62390 36.000 375 205 8 23 371 102 467 2.87e-83 261
MS.gene040643.t1 AT2G30010 37.568 370 198 8 35 375 31 396 1.82e-81 254
MS.gene040643.t1 AT2G30010 37.568 370 198 8 35 375 74 439 4.45e-81 255
MS.gene040643.t1 AT1G73140 39.181 342 186 8 53 375 67 405 1.52e-80 253
MS.gene040643.t1 AT2G40160 38.040 347 191 9 53 375 77 423 1.75e-79 251
MS.gene040643.t1 AT3G14850 38.066 331 189 5 48 371 29 350 2.40e-79 248
MS.gene040643.t1 AT2G40160 37.752 347 192 9 53 375 77 423 3.38e-79 249
MS.gene040643.t1 AT1G60790 36.364 352 200 9 46 375 183 532 4.07e-79 253
MS.gene040643.t1 AT1G78710 34.897 341 203 7 43 375 28 357 9.45e-79 246
MS.gene040643.t1 AT3G55990 37.427 342 193 7 51 371 138 479 5.12e-77 246
MS.gene040643.t1 AT2G40150 36.888 347 192 7 52 371 70 416 5.33e-77 244
MS.gene040643.t1 AT2G38320 33.149 362 219 9 37 375 43 404 2.29e-75 239
MS.gene040643.t1 AT5G19160 36.486 370 203 8 32 375 81 444 2.39e-75 241
MS.gene040643.t1 AT5G01620 35.278 360 211 9 35 375 93 449 4.65e-74 237
MS.gene040643.t1 AT5G01620 35.278 360 211 9 35 375 93 449 4.65e-74 237
MS.gene040643.t1 AT5G01620 35.278 360 211 9 35 375 101 457 5.80e-74 237
MS.gene040643.t1 AT3G11570 35.606 396 214 12 8 375 37 419 3.66e-73 234
MS.gene040643.t1 AT3G06080 36.752 351 196 7 48 375 98 445 7.48e-71 229
MS.gene040643.t1 AT2G40320 34.568 405 219 13 8 375 27 422 1.84e-70 227
MS.gene040643.t1 AT5G06230 37.677 353 187 8 50 375 18 364 9.73e-70 223
MS.gene040643.t1 AT5G06230 37.815 357 181 10 50 375 59 405 1.65e-69 224
MS.gene040643.t1 AT3G11030 35.549 346 197 8 53 375 106 448 5.19e-69 224
MS.gene040643.t1 AT2G31110 40.230 261 151 4 13 270 3 261 5.74e-67 213
MS.gene040643.t1 AT1G48880 35.294 340 188 9 53 375 115 439 3.38e-66 216
MS.gene040643.t1 AT2G38320 33.022 321 194 7 76 375 1 321 4.69e-66 213
MS.gene040643.t1 AT2G30900 42.609 230 127 5 51 277 41 268 1.44e-63 204
MS.gene040643.t1 AT5G58600 40.678 236 134 4 44 277 60 291 1.32e-62 202
MS.gene040643.t1 AT3G62390 36.765 272 155 4 23 277 102 373 3.86e-62 205
MS.gene040643.t1 AT2G34070 37.308 260 146 4 122 375 1 249 2.43e-60 195
MS.gene040643.t1 AT2G40160 40.678 236 129 6 53 277 77 312 3.19e-59 194
MS.gene040643.t1 AT5G19160 37.066 259 147 4 32 277 81 336 6.79e-56 187
MS.gene040643.t1 AT5G20590 38.272 243 137 4 48 277 136 378 2.08e-55 186
MS.gene040643.t1 AT1G78710 30.532 357 180 11 23 375 5 297 7.88e-53 177
MS.gene040643.t1 AT3G06080 35.802 243 143 3 48 277 98 340 7.80e-50 171
MS.gene040643.t1 AT3G14850 35.938 256 149 4 122 371 1 247 2.74e-49 167
MS.gene040643.t1 AT2G31110 40.553 217 113 4 164 375 9 214 3.40e-49 165
MS.gene040643.t1 AT5G06230 37.652 247 135 4 50 277 59 305 5.16e-49 168
MS.gene040643.t1 AT1G70230 31.534 352 193 12 53 375 79 411 5.90e-42 152
MS.gene040643.t1 AT1G01430 28.378 370 228 12 37 375 84 447 4.61e-41 150
MS.gene040643.t1 AT4G25360 31.383 376 208 16 37 375 162 524 7.62e-41 151
MS.gene040643.t1 AT4G25360 31.383 376 208 16 37 375 162 524 7.62e-41 151
MS.gene040643.t1 AT4G01080 28.736 348 215 11 49 371 87 426 1.35e-39 146
MS.gene040643.t1 AT5G64020 29.050 358 213 15 53 375 57 408 1.11e-38 143
MS.gene040643.t1 AT5G51640 29.223 373 218 12 39 375 126 488 2.52e-37 141
MS.gene040643.t1 AT2G37720 29.096 354 210 14 51 375 141 482 1.30e-36 139
MS.gene040643.t1 AT5G20680 27.041 392 232 17 19 375 85 457 1.78e-36 138
MS.gene040643.t1 AT5G20680 27.273 385 226 17 26 375 168 533 3.50e-36 138
MS.gene040643.t1 AT5G64470 27.095 358 228 12 43 375 45 394 3.71e-36 136
MS.gene040643.t1 AT5G20680 27.273 385 226 17 26 375 186 551 4.59e-36 138
MS.gene040643.t1 AT5G20680 27.273 385 226 17 26 375 186 551 4.59e-36 138
MS.gene040643.t1 AT5G20680 27.273 385 226 17 26 375 186 551 4.59e-36 138
MS.gene040643.t1 AT5G20680 27.273 385 226 17 26 375 186 551 4.59e-36 138
MS.gene040643.t1 AT4G23790 27.559 381 215 13 34 371 61 423 4.70e-36 136
MS.gene040643.t1 AT5G64470 26.923 364 227 12 43 375 45 400 1.19e-35 135
MS.gene040643.t1 AT4G11090 29.477 363 204 15 51 375 77 425 1.47e-35 135
MS.gene040643.t1 AT5G15900 28.926 363 224 11 40 375 54 409 1.12e-33 130
MS.gene040643.t1 AT5G15900 28.926 363 224 11 40 375 73 428 1.66e-33 129
MS.gene040643.t1 AT5G15890 28.691 359 209 11 47 375 181 522 4.31e-33 129
MS.gene040643.t1 AT3G02440 28.125 352 214 12 53 375 135 476 6.08e-33 129
MS.gene040643.t1 AT3G02440 28.125 352 214 12 53 375 127 468 7.12e-33 128
MS.gene040643.t1 AT2G14530 27.701 361 213 13 50 375 59 406 3.08e-32 125
MS.gene040643.t1 AT3G28150 28.117 377 209 18 39 375 56 410 5.04e-31 122
MS.gene040643.t1 AT5G64470 26.848 257 169 7 43 282 45 299 4.19e-24 101
MS.gene040643.t1 AT5G64470 26.953 256 168 7 43 281 45 298 6.51e-24 101

Find 81 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTAGATTCTCTATTGTTT+TGG 0.217386 1.2:+33343860 None:intergenic
TAATACCAACTGGTAGAAAA+AGG 0.249328 1.2:-33342125 MS.gene040643:CDS
CTTATGGTTGCTTATCAAAA+AGG 0.263528 1.2:-33341413 MS.gene040643:CDS
CCACTGCACATTATCTCTTC+TGG 0.264239 1.2:-33343935 MS.gene040643:CDS
TTTGATGCACTAAGGTTTCT+TGG 0.268531 1.2:-33342234 MS.gene040643:CDS
TCCCTGGTGTGCCTGATATA+TGG 0.296302 1.2:-33340817 MS.gene040643:CDS
GCTGAAGAAAATGAGGTTTC+CGG 0.326494 1.2:-33340969 MS.gene040643:CDS
GTTCAGGAGCATGTTGTTGC+AGG 0.359962 1.2:+33341014 None:intergenic
AACATCTCATTCCATATATC+AGG 0.370214 1.2:+33340806 None:intergenic
TGGACAATGTTAACATCATC+AGG 0.381677 1.2:+33343880 None:intergenic
GCATGTCACCTAGGCATAAC+AGG 0.386311 1.2:-33341322 MS.gene040643:intron
AAACAAGAGAGTCTTACATT+TGG 0.395362 1.2:-33341919 MS.gene040643:CDS
CATGCTTCTGAAAATGACTT+TGG 0.398420 1.2:+33341340 None:intergenic
ATTCATCTGATTGCAGTCAT+TGG 0.399784 1.2:-33340844 MS.gene040643:CDS
CACTTCTCATAATCAAAATC+TGG 0.400310 1.2:+33343727 None:intergenic
ATTGAATGGAAGGCCATTGC+AGG 0.414061 1.2:+33342087 None:intergenic
TGCAGTCATTGGTGCCTCCC+TGG 0.417455 1.2:-33340833 MS.gene040643:CDS
CTTACATTTGGATTTGATTG+AGG 0.428799 1.2:-33341907 MS.gene040643:CDS
TCATTGTCGTAATATCTTGC+AGG 0.431750 1.2:+33340942 None:intergenic
ATGTCTTCATTACCATTGAA+TGG 0.434469 1.2:+33342073 None:intergenic
TGGACCCACAAGATGATGTT+GGG 0.437455 1.2:-33343915 MS.gene040643:CDS
AAAGAAAGCAAATGAGTCAT+TGG 0.443285 1.2:+33343979 None:intergenic
TTGAGGAGAATGCAAAGTAT+TGG 0.448978 1.2:-33341890 MS.gene040643:CDS
GCAGTTACTTGTCAAAAGAA+TGG 0.450896 1.2:-33343754 MS.gene040643:CDS
TTCCATATATCAGGCACACC+AGG 0.460916 1.2:+33340815 None:intergenic
ATGGACATCCTTCAGTGTAT+AGG 0.463306 1.2:-33340904 MS.gene040643:CDS
TGGAGAACAACTAAAGGTTC+AGG 0.465074 1.2:+33340998 None:intergenic
GTAACTGCAGTACTAAGATA+AGG 0.467342 1.2:+33343769 None:intergenic
TCTGTAGGTTTGATGCACTA+AGG 0.472754 1.2:-33342242 MS.gene040643:intron
TGCAATGGCCTTCCATTCAA+TGG 0.483402 1.2:-33342085 MS.gene040643:intron
GTACTAAGATAAGGGCAGTT+AGG 0.483942 1.2:+33343778 None:intergenic
CAGTTACTTGTCAAAAGAAT+GGG 0.497842 1.2:-33343753 MS.gene040643:CDS
CTGGACCCACAAGATGATGT+TGG 0.501831 1.2:-33343916 MS.gene040643:CDS
GTACAAGGTGTAATACCAAC+TGG 0.502784 1.2:-33342135 MS.gene040643:CDS
TAACTGCAGTACTAAGATAA+GGG 0.511515 1.2:+33343770 None:intergenic
GTAATATCTTGCAGGTATAC+CGG 0.515454 1.2:+33340950 None:intergenic
CTTCATTACCATTGAATGGA+AGG 0.525435 1.2:+33342077 None:intergenic
TATTTGATTCAGCTCAGTGG+TGG 0.527529 1.2:-33341848 MS.gene040643:CDS
TTGATTATGAGAAGTGGAAG+TGG 0.528886 1.2:-33343720 MS.gene040643:CDS
TTCAATAATGAGAAATCAGT+GGG 0.529697 1.2:-33342175 MS.gene040643:CDS
GAAACCCAACATCATCTTGT+GGG 0.534722 1.2:+33343911 None:intergenic
TTCTTCAGTTCAACTAACAA+TGG 0.540448 1.2:+33341951 None:intergenic
AATGACTGCAATCAGATGAA+TGG 0.543715 1.2:+33340846 None:intergenic
ACTGAGTACATGGGCAAGGT+GGG 0.549939 1.2:-33341390 MS.gene040643:CDS
GGGCAGTTAGGATCATAAAG+AGG 0.560599 1.2:+33343790 None:intergenic
AGGAAGAGAATCATGTTGGT+GGG 0.564160 1.2:-33342201 MS.gene040643:CDS
ACTCCTTGGAGAACAACTAA+AGG 0.569021 1.2:+33340992 None:intergenic
GGAAGGCCATTGCAGGTCCA+TGG 0.570480 1.2:+33342094 None:intergenic
GGCAGTTAGGATCATAAAGA+GGG 0.572794 1.2:+33343791 None:intergenic
GACCTACCATGGACCTGCAA+TGG 0.573638 1.2:-33342100 MS.gene040643:CDS
GAGAAGGAAGAGAATCATGT+TGG 0.573655 1.2:-33342205 MS.gene040643:CDS
ATATAGTACCTGTTATGCCT+AGG 0.573860 1.2:+33341314 None:intergenic
AAGGAGTGCTGAAGAAAATG+AGG 0.580576 1.2:-33340976 MS.gene040643:CDS
GAGTACATGGGCAAGGTGGG+TGG 0.582998 1.2:-33341387 MS.gene040643:CDS
TCTTTAGTATGCTTAGTACA+AGG 0.583313 1.2:-33342150 MS.gene040643:CDS
GAGAATGCAAAGTATTGGAG+AGG 0.588312 1.2:-33341885 MS.gene040643:CDS
CTAGTGGTTGTTCTATCCCA+AGG 0.588736 1.2:-33343693 MS.gene040643:intron
GACTGAGTACATGGGCAAGG+TGG 0.591699 1.2:-33341391 MS.gene040643:CDS
ATGAGTCATTGGTGTTGCCA+TGG 0.591707 1.2:+33343990 None:intergenic
TTGTATTTGATTCAGCTCAG+TGG 0.592724 1.2:-33341851 MS.gene040643:CDS
GAACCTTTAGTTGTTCTCCA+AGG 0.596967 1.2:-33340995 MS.gene040643:CDS
AGGATGTCCATCTCTGCGTA+AGG 0.608878 1.2:+33340916 None:intergenic
AGAAACCCAACATCATCTTG+TGG 0.610318 1.2:+33343910 None:intergenic
GGCCATTGCAGGTCCATGGT+AGG 0.611844 1.2:+33342098 None:intergenic
GTTGCCTTCCTATACACTGA+AGG 0.612680 1.2:+33340896 None:intergenic
AAGGAAGAGAATCATGTTGG+TGG 0.613045 1.2:-33342202 MS.gene040643:CDS
ATGACCGCCTTACGCAGAGA+TGG 0.614118 1.2:-33340923 MS.gene040643:CDS
ATTCAATAATGAGAAATCAG+TGG 0.617219 1.2:-33342176 MS.gene040643:CDS
ATACAGGTGGGATTACTACA+AGG 0.634100 1.2:-33341465 MS.gene040643:intron
ACTGAGCTGAATCAAATACA+AGG 0.634640 1.2:+33341853 None:intergenic
CTTGGGATAGAACAACCACT+AGG 0.637657 1.2:+33343694 None:intergenic
TCCATATATCAGGCACACCA+GGG 0.647522 1.2:+33340816 None:intergenic
AGGTGGGATTACTACAAGGA+GGG 0.648410 1.2:-33341461 MS.gene040643:CDS
AAGGACTGAGTACATGGGCA+AGG 0.650632 1.2:-33341394 MS.gene040643:CDS
ATGTCCATCTCTGCGTAAGG+CGG 0.652540 1.2:+33340919 None:intergenic
CCAGAAGAGATAATGTGCAG+TGG 0.657294 1.2:+33343935 None:intergenic
TTACTTGTCAAAAGAATGGG+AGG 0.658568 1.2:-33343750 MS.gene040643:CDS
ACATCCTTCAGTGTATAGGA+AGG 0.659861 1.2:-33340900 MS.gene040643:CDS
CAGGTGGGATTACTACAAGG+AGG 0.703135 1.2:-33341462 MS.gene040643:CDS
AAGTGGAAGTGGAAGCCTAG+TGG 0.748991 1.2:-33343709 MS.gene040643:CDS
ATATATCAGGCACACCAGGG+AGG 0.777258 1.2:+33340819 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTGTTATTGTTTTTTATA+GGG - chr1.2:33341278-33341297 MS.gene040643:intron 10.0%
!!! CAAAATATAAATTTTATCTA+AGG - chr1.2:33341438-33341457 MS.gene040643:CDS 10.0%
!! AGTTCTTAAAATAAGTAAAA+TGG + chr1.2:33342375-33342394 None:intergenic 15.0%
!! CATTTACTTAAATACAAATT+TGG + chr1.2:33342458-33342477 None:intergenic 15.0%
!!! AACTTTGTTTATCAATTAAA+TGG + chr1.2:33341596-33341615 None:intergenic 15.0%
!!! ACTTGTTAAAGATTAAAAAA+AGG + chr1.2:33341534-33341553 None:intergenic 15.0%
!!! AGTATATTCTAGATTTTTTA+GGG - chr1.2:33341882-33341901 MS.gene040643:CDS 15.0%
!!! GATTTGTTATTGTTTTTTAT+AGG - chr1.2:33341277-33341296 MS.gene040643:intron 15.0%
!!! TAGTATATTCTAGATTTTTT+AGG - chr1.2:33341881-33341900 MS.gene040643:CDS 15.0%
!!! TTTGTTATTGTTTTTTATAG+GGG - chr1.2:33341279-33341298 MS.gene040643:intron 15.0%
!! AGATTTATCATTCAAATTTG+TGG - chr1.2:33341916-33341935 MS.gene040643:CDS 20.0%
!! CTTAAAATAAGTAAAATGGA+AGG + chr1.2:33342371-33342390 None:intergenic 20.0%
!! TGATTTCCGATAAAATATTA+TGG + chr1.2:33343587-33343606 None:intergenic 20.0%
!!! ACAACACCATAATATTTTAT+CGG - chr1.2:33343578-33343597 MS.gene040643:intron 20.0%
!!! ATTGAGTTCTAAAAAAAACA+TGG - chr1.2:33341807-33341826 MS.gene040643:intron 20.0%
!!! GTATATTCTAGATTTTTTAG+GGG - chr1.2:33341883-33341902 MS.gene040643:CDS 20.0%
!!! TTGTGTTATTGTAATTTGTA+CGG - chr1.2:33342094-33342113 MS.gene040643:CDS 20.0%
!!! TTGTTATTGTTTTTTATAGG+GGG - chr1.2:33341280-33341299 MS.gene040643:intron 20.0%
!!! TTTTTTTAGAACTCAATATC+CGG + chr1.2:33341804-33341823 None:intergenic 20.0%
! AAAATAAGTAAAATGGAAGG+TGG + chr1.2:33342368-33342387 None:intergenic 25.0%
! AAACACTGAATTATGTAGTT+TGG + chr1.2:33343052-33343071 None:intergenic 25.0%
! AACTATCTTAGAAAGCTTAT+GGG - chr1.2:33343173-33343192 MS.gene040643:intron 25.0%
! AATGCCAAAAACAAAACTAT+AGG + chr1.2:33343660-33343679 None:intergenic 25.0%
! ATTCAATAATGAGAAATCAG+TGG - chr1.2:33342602-33342621 MS.gene040643:intron 25.0%
! CAAGATGAGAAACAAAAAAT+AGG - chr1.2:33343909-33343928 MS.gene040643:CDS 25.0%
! GATAAAAAACATGACTCTTA+TGG - chr1.2:33343349-33343368 MS.gene040643:intron 25.0%
! GTAAATGAATTTCAACACTT+TGG - chr1.2:33342471-33342490 MS.gene040643:intron 25.0%
! GTGATGTCAATAATTTACAA+AGG + chr1.2:33342759-33342778 None:intergenic 25.0%
! GTGTATAGAAGAAAACAAAA+AGG + chr1.2:33341127-33341146 None:intergenic 25.0%
! GTTCTTGAAATTGTAAATGT+CGG + chr1.2:33342436-33342455 None:intergenic 25.0%
! TAACTATCTTAGAAAGCTTA+TGG - chr1.2:33343172-33343191 MS.gene040643:intron 25.0%
! TATAGAAGAAAACAAAAAGG+TGG + chr1.2:33341124-33341143 None:intergenic 25.0%
! TGTATTTACTTGGTCAAAAT+TGG - chr1.2:33341221-33341240 MS.gene040643:intron 25.0%
! TGTCAATAATTTACAAAGGA+AGG + chr1.2:33342755-33342774 None:intergenic 25.0%
! TTCAATAATGAGAAATCAGT+GGG - chr1.2:33342603-33342622 MS.gene040643:intron 25.0%
! TTTATCAATTAAATGGATGC+AGG + chr1.2:33341589-33341608 None:intergenic 25.0%
! TTTGTGGATTCTTATTAATG+TGG - chr1.2:33342306-33342325 MS.gene040643:intron 25.0%
!! ACAAATATTTTCAGCCAAAA+TGG + chr1.2:33342291-33342310 None:intergenic 25.0%
!! AGACATCAATTGAGTTTTTA+TGG - chr1.2:33342801-33342820 MS.gene040643:intron 25.0%
!! GACAAAGATAAACAAGTTTT+AGG + chr1.2:33343501-33343520 None:intergenic 25.0%
!! GACATCAATTGAGTTTTTAT+GGG - chr1.2:33342802-33342821 MS.gene040643:intron 25.0%
!! GGAAACAATTGACAAATTTT+AGG + chr1.2:33341513-33341532 None:intergenic 25.0%
!! TATTGTAATTTGTACGGATA+TGG - chr1.2:33342100-33342119 MS.gene040643:CDS 25.0%
!!! AAAATGACTTTGGTTTTTTG+AGG + chr1.2:33343431-33343450 None:intergenic 25.0%
!!! AAATGACTTTGGTTTTTTGA+GGG + chr1.2:33343430-33343449 None:intergenic 25.0%
!!! AGTGATTGGATTTTTTATGT+AGG - chr1.2:33343691-33343710 MS.gene040643:intron 25.0%
!!! AGTTCTAAAAAAAACATGGT+TGG - chr1.2:33341811-33341830 MS.gene040643:intron 25.0%
!!! ATTTTGGCTGAAAATATTTG+TGG - chr1.2:33342290-33342309 MS.gene040643:intron 25.0%
!!! CTATTTTTTGTTTCTCATCT+TGG + chr1.2:33343911-33343930 None:intergenic 25.0%
!!! GATTTTTTATGTAGGGAAAA+TGG - chr1.2:33343699-33343718 MS.gene040643:CDS 25.0%
!!! GTGATTGGATTTTTTATGTA+GGG - chr1.2:33343692-33343711 MS.gene040643:intron 25.0%
!!! TTTTTTATCAACTCTGAGTA+GGG - chr1.2:33341974-33341993 MS.gene040643:CDS 25.0%
AAACAAGAGAGTCTTACATT+TGG - chr1.2:33342859-33342878 MS.gene040643:intron 30.0%
AAAGAAAGCAAATGAGTCAT+TGG + chr1.2:33340802-33340821 None:intergenic 30.0%
AACATCTCATTCCATATATC+AGG + chr1.2:33343975-33343994 None:intergenic 30.0%
AAGTTATGCATATAGGAGAT+GGG + chr1.2:33342059-33342078 None:intergenic 30.0%
AATAAGCCAAAATCAGCTTA+TGG - chr1.2:33343196-33343215 MS.gene040643:intron 30.0%
AATACCAACTGGTAGAAAAA+GGG - chr1.2:33342654-33342673 MS.gene040643:intron 30.0%
ACTATGGAAGTTATGCATAT+AGG + chr1.2:33342066-33342085 None:intergenic 30.0%
ACTTAGTGAGATATAAGACT+TGG + chr1.2:33343537-33343556 None:intergenic 30.0%
AGTAAATACATGTGTCATGA+TGG + chr1.2:33341211-33341230 None:intergenic 30.0%
ATCACAAGATTGTGATACTT+AGG - chr1.2:33341308-33341327 MS.gene040643:intron 30.0%
ATGTCTTCATTACCATTGAA+TGG + chr1.2:33342708-33342727 None:intergenic 30.0%
ATTAGATCTCTCGAATTAGT+CGG - chr1.2:33341770-33341789 MS.gene040643:intron 30.0%
ATTTCATTGTCTCTAGTGAT+TGG - chr1.2:33343677-33343696 MS.gene040643:intron 30.0%
CACTTCTCATAATCAAAATC+TGG + chr1.2:33341054-33341073 None:intergenic 30.0%
CAGTTACTTGTCAAAAGAAT+GGG - chr1.2:33341025-33341044 MS.gene040643:CDS 30.0%
CTTACATTTGGATTTGATTG+AGG - chr1.2:33342871-33342890 MS.gene040643:intron 30.0%
CTTATGGTTGCTTATCAAAA+AGG - chr1.2:33343365-33343384 MS.gene040643:intron 30.0%
CTTATTAACAAATCCATGAG+AGG + chr1.2:33341416-33341435 None:intergenic 30.0%
GACTAACACTTACAATTTCA+GGG + chr1.2:33342175-33342194 None:intergenic 30.0%
GATTCCTTCTGTATCAATTT+CGG - chr1.2:33341686-33341705 MS.gene040643:intron 30.0%
GTTAAGATTTAGAAGCTCAA+TGG + chr1.2:33343628-33343647 None:intergenic 30.0%
TAACACAAAAACCATGACTA+TGG + chr1.2:33342082-33342101 None:intergenic 30.0%
TAACTGCAGTACTAAGATAA+GGG + chr1.2:33341011-33341030 None:intergenic 30.0%
TAATACCAACTGGTAGAAAA+AGG - chr1.2:33342653-33342672 MS.gene040643:intron 30.0%
TAATCTCATTGTTCACTTGT+GGG + chr1.2:33341754-33341773 None:intergenic 30.0%
TCACAAGATTGTGATACTTA+GGG - chr1.2:33341309-33341328 MS.gene040643:intron 30.0%
TCATGACACATGTATTTACT+TGG - chr1.2:33341211-33341230 MS.gene040643:intron 30.0%
TCTTTAGTATGCTTAGTACA+AGG - chr1.2:33342628-33342647 MS.gene040643:intron 30.0%
TGACTAACACTTACAATTTC+AGG + chr1.2:33342176-33342195 None:intergenic 30.0%
TTCTTCAGTTCAACTAACAA+TGG + chr1.2:33342830-33342849 None:intergenic 30.0%
TTTCATGTGCATAACAATGA+TGG + chr1.2:33341362-33341381 None:intergenic 30.0%
! AAAAGATGTGACTTTTCTGT+TGG - chr1.2:33340940-33340959 MS.gene040643:CDS 30.0%
! ACTAAGTGAAGTTGATTACA+TGG - chr1.2:33343550-33343569 MS.gene040643:intron 30.0%
! AGTTGCCTTTTCATGAAATT+TGG + chr1.2:33342208-33342227 None:intergenic 30.0%
! CATAGGTTTAATTGCAGTTT+TGG + chr1.2:33341337-33341356 None:intergenic 30.0%
! CTATTTTCTCAATTTCCCTT+GGG - chr1.2:33343026-33343045 MS.gene040643:intron 30.0%
! GGATTAAAATCTGAGATCAA+TGG + chr1.2:33342038-33342057 None:intergenic 30.0%
! GTATCACAATCTTGTGATTT+TGG + chr1.2:33341306-33341325 None:intergenic 30.0%
! GTTGCCTTTTCATGAAATTT+GGG + chr1.2:33342207-33342226 None:intergenic 30.0%
! TAAAATCTGAGATCAATGGA+GGG + chr1.2:33342034-33342053 None:intergenic 30.0%
! TCTATTTTCTCAATTTCCCT+TGG - chr1.2:33343025-33343044 MS.gene040643:intron 30.0%
! TGACTTTTCTGTTGGAAAAT+GGG - chr1.2:33340948-33340967 MS.gene040643:CDS 30.0%
! TGCTAGATTCTCTATTGTTT+TGG + chr1.2:33340921-33340940 None:intergenic 30.0%
! TTAAAATCTGAGATCAATGG+AGG + chr1.2:33342035-33342054 None:intergenic 30.0%
! TTGCCTTTTCATGAAATTTG+GGG + chr1.2:33342206-33342225 None:intergenic 30.0%
! TTTTATGTAGGGAAAATGGT+TGG - chr1.2:33343703-33343722 MS.gene040643:CDS 30.0%
! TTTTTATCAACTCTGAGTAG+GGG - chr1.2:33341975-33341994 MS.gene040643:CDS 30.0%
!!! CAGATTTTGATTATGAGAAG+TGG - chr1.2:33341052-33341071 MS.gene040643:CDS 30.0%
!!! GTTTTTTATCAACTCTGAGT+AGG - chr1.2:33341973-33341992 MS.gene040643:CDS 30.0%
!!! TTCGCCTATAGTTTTGTTTT+TGG - chr1.2:33343653-33343672 MS.gene040643:intron 30.0%
AATAGAACGATGTGAAGAGA+AGG + chr1.2:33343011-33343030 None:intergenic 35.0%
AATCTCATTGTTCACTTGTG+GGG + chr1.2:33341753-33341772 None:intergenic 35.0%
AATGACTGCAATCAGATGAA+TGG + chr1.2:33343935-33343954 None:intergenic 35.0%
AATTATGTAGTTTGGCCCAA+GGG + chr1.2:33343044-33343063 None:intergenic 35.0%
ACAAGATTGTGATACTTAGG+GGG - chr1.2:33341311-33341330 MS.gene040643:intron 35.0%
ACTGAGCTGAATCAAATACA+AGG + chr1.2:33342928-33342947 None:intergenic 35.0%
AGCTAGTTAAGTTTACAAGC+AGG + chr1.2:33342509-33342528 None:intergenic 35.0%
AGTCCCCAAATTTCATGAAA+AGG - chr1.2:33342200-33342219 MS.gene040643:CDS 35.0%
ATATAGTACCTGTTATGCCT+AGG + chr1.2:33343467-33343486 None:intergenic 35.0%
ATCAAAAAGGACTGAGTACA+TGG - chr1.2:33343378-33343397 MS.gene040643:intron 35.0%
ATGCATAACTTCCATAGTCA+TGG - chr1.2:33342068-33342087 MS.gene040643:intron 35.0%
ATTCATCTGATTGCAGTCAT+TGG - chr1.2:33343934-33343953 MS.gene040643:CDS 35.0%
CAAAATCAGCTTATGGACAT+GGG - chr1.2:33343203-33343222 MS.gene040643:intron 35.0%
CACAAGATTGTGATACTTAG+GGG - chr1.2:33341310-33341329 MS.gene040643:intron 35.0%
CTAATCTCATTGTTCACTTG+TGG + chr1.2:33341755-33341774 None:intergenic 35.0%
CTTCATTACCATTGAATGGA+AGG + chr1.2:33342704-33342723 None:intergenic 35.0%
CTTTCTTGACTAATACAGGT+GGG - chr1.2:33343301-33343320 MS.gene040643:intron 35.0%
GAAGTTATGCATATAGGAGA+TGG + chr1.2:33342060-33342079 None:intergenic 35.0%
GATCTCAAATCAACGTCAAT+AGG + chr1.2:33341635-33341654 None:intergenic 35.0%
GCAGTTACTTGTCAAAAGAA+TGG - chr1.2:33341024-33341043 MS.gene040643:CDS 35.0%
GCTTCTTTCTTGACTAATAC+AGG - chr1.2:33343297-33343316 MS.gene040643:intron 35.0%
GGTTTCTTGGAAAAATGAGA+AGG - chr1.2:33342557-33342576 MS.gene040643:intron 35.0%
GTAACTGCAGTACTAAGATA+AGG + chr1.2:33341012-33341031 None:intergenic 35.0%
GTAATATCTTGCAGGTATAC+CGG + chr1.2:33343831-33343850 None:intergenic 35.0%
TAATCTTTAACAAGTGCCCT+CGG - chr1.2:33341539-33341558 MS.gene040643:intron 35.0%
TACTATGACTACTTGTGTCA+AGG + chr1.2:33341866-33341885 None:intergenic 35.0%
TCAAAAAGGACTGAGTACAT+GGG - chr1.2:33343379-33343398 MS.gene040643:intron 35.0%
TCATTGTCGTAATATCTTGC+AGG + chr1.2:33343839-33343858 None:intergenic 35.0%
TCTTTCTTGACTAATACAGG+TGG - chr1.2:33343300-33343319 MS.gene040643:intron 35.0%
TGGACAATGTTAACATCATC+AGG + chr1.2:33340901-33340920 None:intergenic 35.0%
TTACTTGTCAAAAGAATGGG+AGG - chr1.2:33341028-33341047 MS.gene040643:CDS 35.0%
TTGAGGAGAATGCAAAGTAT+TGG - chr1.2:33342888-33342907 MS.gene040643:intron 35.0%
TTGATTATGAGAAGTGGAAG+TGG - chr1.2:33341058-33341077 MS.gene040643:CDS 35.0%
TTGTATTTGATTCAGCTCAG+TGG - chr1.2:33342927-33342946 MS.gene040643:intron 35.0%
TTTGATGCACTAAGGTTTCT+TGG - chr1.2:33342544-33342563 MS.gene040643:intron 35.0%
! AACTAGCTTATTACCTCTGT+AGG - chr1.2:33342521-33342540 MS.gene040643:intron 35.0%
! ATTGACATCACAGTTTTCTC+TGG - chr1.2:33342768-33342787 MS.gene040643:intron 35.0%
! CAAAAGGGTGTAAAGTTAGA+GGG - chr1.2:33341252-33341271 MS.gene040643:intron 35.0%
! CATGCTTCTGAAAATGACTT+TGG + chr1.2:33343441-33343460 None:intergenic 35.0%
! CTTAGAGTGTGCTTTTCTTA+AGG - chr1.2:33341654-33341673 MS.gene040643:intron 35.0%
! GTGACTTTTCTGTTGGAAAA+TGG - chr1.2:33340947-33340966 MS.gene040643:CDS 35.0%
! GTGGATGATGAGTTTTTGAA+TGG - chr1.2:33342130-33342149 MS.gene040643:CDS 35.0%
!! GCATAACAATGATGGATCAT+AGG + chr1.2:33341354-33341373 None:intergenic 35.0%
!! GTCGATTAGCTTTCTAAAAG+AGG - chr1.2:33343235-33343254 MS.gene040643:intron 35.0%
!! TTTTTGTTTCTCATCTTGGC+TGG + chr1.2:33343907-33343926 None:intergenic 35.0%
!!! TCCTTACGAGCTTGTTTTAT+CGG + chr1.2:33342963-33342982 None:intergenic 35.0%
!!! TGTAAGACTCTCTTGTTTTC+TGG + chr1.2:33342857-33342876 None:intergenic 35.0%
AAGGAAGAGAATCATGTTGG+TGG - chr1.2:33342576-33342595 MS.gene040643:intron 40.0%
AATGTTAACGTGTCCTCTCA+TGG - chr1.2:33341400-33341419 MS.gene040643:CDS 40.0%
ACATCCTTCAGTGTATAGGA+AGG - chr1.2:33343878-33343897 MS.gene040643:CDS 40.0%
ACTCCTTGGAGAACAACTAA+AGG + chr1.2:33343789-33343808 None:intergenic 40.0%
AGAAACCCAACATCATCTTG+TGG + chr1.2:33340871-33340890 None:intergenic 40.0%
AGATTTAGAAGCTCAATGGC+TGG + chr1.2:33343624-33343643 None:intergenic 40.0%
AGGAAGAGAATCATGTTGGT+GGG - chr1.2:33342577-33342596 MS.gene040643:intron 40.0%
ATACAGGTGGGATTACTACA+AGG - chr1.2:33343313-33343332 MS.gene040643:intron 40.0%
ATCTCATTGTTCACTTGTGG+GGG + chr1.2:33341752-33341771 None:intergenic 40.0%
ATGGACATCCTTCAGTGTAT+AGG - chr1.2:33343874-33343893 MS.gene040643:CDS 40.0%
CCAAAATCAGCTTATGGACA+TGG - chr1.2:33343202-33343221 MS.gene040643:intron 40.0%
GAAACCCAACATCATCTTGT+GGG + chr1.2:33340870-33340889 None:intergenic 40.0%
GAACCTTTAGTTGTTCTCCA+AGG - chr1.2:33343783-33343802 MS.gene040643:CDS 40.0%
GAATTATGTAGTTTGGCCCA+AGG + chr1.2:33343045-33343064 None:intergenic 40.0%
GAGAAGGAAGAGAATCATGT+TGG - chr1.2:33342573-33342592 MS.gene040643:intron 40.0%
GCTGAAGAAAATGAGGTTTC+CGG - chr1.2:33343809-33343828 MS.gene040643:CDS 40.0%
GGCAGTTAGGATCATAAAGA+GGG + chr1.2:33340990-33341009 None:intergenic 40.0%
GTACAAGGTGTAATACCAAC+TGG - chr1.2:33342643-33342662 MS.gene040643:intron 40.0%
GTACTAAGATAAGGGCAGTT+AGG + chr1.2:33341003-33341022 None:intergenic 40.0%
TATTTGATTCAGCTCAGTGG+TGG - chr1.2:33342930-33342949 MS.gene040643:intron 40.0%
TCCACCGAAATTGATACAGA+AGG + chr1.2:33341693-33341712 None:intergenic 40.0%
TCCGATAAAACAAGCTCGTA+AGG - chr1.2:33342959-33342978 MS.gene040643:intron 40.0%
TCCTTCTGTATCAATTTCGG+TGG - chr1.2:33341689-33341708 MS.gene040643:intron 40.0%
TCTCGAATTAGTCGGTCTTT+AGG - chr1.2:33341778-33341797 MS.gene040643:intron 40.0%
TCTGTAGGTTTGATGCACTA+AGG - chr1.2:33342536-33342555 MS.gene040643:intron 40.0%
TGAAATTCTAAGTGCTCCGA+GGG + chr1.2:33341558-33341577 None:intergenic 40.0%
TGGAGAACAACTAAAGGTTC+AGG + chr1.2:33343783-33343802 None:intergenic 40.0%
TGTTGTTGCAGGCTGAAAAA+TGG + chr1.2:33343756-33343775 None:intergenic 40.0%
TTAGTGCATCAAACCTACAG+AGG + chr1.2:33342537-33342556 None:intergenic 40.0%
TTGAAATTCTAAGTGCTCCG+AGG + chr1.2:33341559-33341578 None:intergenic 40.0%
! AAAATTGGTCTGTGGCCAAA+AGG - chr1.2:33341236-33341255 MS.gene040643:intron 40.0%
! AAATTGGTCTGTGGCCAAAA+GGG - chr1.2:33341237-33341256 MS.gene040643:intron 40.0%
! ACAACTCACAAGGACCATTT+TGG - chr1.2:33342274-33342293 MS.gene040643:intron 40.0%
! ACATCACAGTTTTCTCTGGT+AGG - chr1.2:33342772-33342791 MS.gene040643:intron 40.0%
! ACTTGGTCAAAATTGGTCTG+TGG - chr1.2:33341228-33341247 MS.gene040643:intron 40.0%
! CCAAAAGGGTGTAAAGTTAG+AGG - chr1.2:33341251-33341270 MS.gene040643:intron 40.0%
! CCTCTAACTTTACACCCTTT+TGG + chr1.2:33341254-33341273 None:intergenic 40.0%
! TCATTTTCTTCAGCACTCCT+TGG + chr1.2:33343803-33343822 None:intergenic 40.0%
!! AAGGAGTGCTGAAGAAAATG+AGG - chr1.2:33343802-33343821 MS.gene040643:CDS 40.0%
!! CCATGTCCATAAGCTGATTT+TGG + chr1.2:33343205-33343224 None:intergenic 40.0%
!! GAGAATGCAAAGTATTGGAG+AGG - chr1.2:33342893-33342912 MS.gene040643:intron 40.0%
!! TTTTCAGAAGCATGTCACCT+AGG - chr1.2:33343447-33343466 MS.gene040643:intron 40.0%
AGAAAAAGGGTGACCTACCA+TGG - chr1.2:33342667-33342686 MS.gene040643:intron 45.0%
AGAACGATGTGAAGAGAAGG+TGG + chr1.2:33343008-33343027 None:intergenic 45.0%
AGGTGGGATTACTACAAGGA+GGG - chr1.2:33343317-33343336 MS.gene040643:intron 45.0%
ATTGAATGGAAGGCCATTGC+AGG + chr1.2:33342694-33342713 None:intergenic 45.0%
CCACTGCACATTATCTCTTC+TGG - chr1.2:33340843-33340862 MS.gene040643:CDS 45.0%
CCAGAAGAGATAATGTGCAG+TGG + chr1.2:33340846-33340865 None:intergenic 45.0%
CTAGTGGTTGTTCTATCCCA+AGG - chr1.2:33341085-33341104 MS.gene040643:intron 45.0%
CTTGGGATAGAACAACCACT+AGG + chr1.2:33341087-33341106 None:intergenic 45.0%
GAATTAGTCGGTCTTTAGGC+CGG - chr1.2:33341782-33341801 MS.gene040643:intron 45.0%
GGGCAGTTAGGATCATAAAG+AGG + chr1.2:33340991-33341010 None:intergenic 45.0%
GTACGGATATGGTAAGCTTG+TGG - chr1.2:33342111-33342130 MS.gene040643:CDS 45.0%
GTTGCCTTCCTATACACTGA+AGG + chr1.2:33343885-33343904 None:intergenic 45.0%
TCCATATATCAGGCACACCA+GGG + chr1.2:33343965-33343984 None:intergenic 45.0%
TGCAATGGCCTTCCATTCAA+TGG - chr1.2:33342693-33342712 MS.gene040643:intron 45.0%
TGCAGGCTGAAAAATGGTAG+AGG + chr1.2:33343750-33343769 None:intergenic 45.0%
TGGACCCACAAGATGATGTT+GGG - chr1.2:33340863-33340882 MS.gene040643:CDS 45.0%
TGGCAATGTGAACAGACCTT+GGG + chr1.2:33341104-33341123 None:intergenic 45.0%
TTCCATATATCAGGCACACC+AGG + chr1.2:33343966-33343985 None:intergenic 45.0%
TTGCGAAGTCACAACTCACA+AGG - chr1.2:33342264-33342283 MS.gene040643:intron 45.0%
! ATCTGAGATCAATGGAGGGT+TGG + chr1.2:33342030-33342049 None:intergenic 45.0%
! GTCACCCTTTTTCTACCAGT+TGG + chr1.2:33342661-33342680 None:intergenic 45.0%
! TAGAAGCTCAATGGCTGGTT+TGG + chr1.2:33343619-33343638 None:intergenic 45.0%
AAGGACTGAGTACATGGGCA+AGG - chr1.2:33343384-33343403 MS.gene040643:intron 50.0%
AAGTGGAAGTGGAAGCCTAG+TGG - chr1.2:33341069-33341088 MS.gene040643:intron 50.0%
ACTGAGTACATGGGCAAGGT+GGG - chr1.2:33343388-33343407 MS.gene040643:intron 50.0%
AGGATGTCCATCTCTGCGTA+AGG + chr1.2:33343865-33343884 None:intergenic 50.0%
ATATATCAGGCACACCAGGG+AGG + chr1.2:33343962-33343981 None:intergenic 50.0%
ATCAACTCTGAGTAGGGGAG+TGG - chr1.2:33341980-33341999 MS.gene040643:CDS 50.0%
ATGTCCATCTCTGCGTAAGG+CGG + chr1.2:33343862-33343881 None:intergenic 50.0%
CAGGTGGGATTACTACAAGG+AGG - chr1.2:33343316-33343335 MS.gene040643:intron 50.0%
CTGGACCCACAAGATGATGT+TGG - chr1.2:33340862-33340881 MS.gene040643:CDS 50.0%
GCATGTCACCTAGGCATAAC+AGG - chr1.2:33343456-33343475 MS.gene040643:intron 50.0%
GTGGCAATGTGAACAGACCT+TGG + chr1.2:33341105-33341124 None:intergenic 50.0%
TCCCTGGTGTGCCTGATATA+TGG - chr1.2:33343961-33343980 MS.gene040643:CDS 50.0%
TGAGTAGGGGAGTGGAATGT+GGG - chr1.2:33341988-33342007 MS.gene040643:intron 50.0%
! GTTCAGGAGCATGTTGTTGC+AGG + chr1.2:33343767-33343786 None:intergenic 50.0%
ATGACCGCCTTACGCAGAGA+TGG - chr1.2:33343855-33343874 MS.gene040643:CDS 55.0%
CTGAGTAGGGGAGTGGAATG+TGG - chr1.2:33341987-33342006 MS.gene040643:CDS 55.0%
GACCTACCATGGACCTGCAA+TGG - chr1.2:33342678-33342697 MS.gene040643:intron 55.0%
GACTGAGTACATGGGCAAGG+TGG - chr1.2:33343387-33343406 MS.gene040643:intron 55.0%
GAGTACATGGGCAAGGTGGG+TGG - chr1.2:33343391-33343410 MS.gene040643:intron 60.0%
GGAAGGCCATTGCAGGTCCA+TGG + chr1.2:33342687-33342706 None:intergenic 60.0%
GGCCATTGCAGGTCCATGGT+AGG + chr1.2:33342683-33342702 None:intergenic 60.0%
! TGCAGTCATTGGTGCCTCCC+TGG - chr1.2:33343945-33343964 MS.gene040643:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 33340790 33344010 33340790 ID=MS.gene040643
chr1.2 mRNA 33340790 33344010 33340790 ID=MS.gene040643.t1;Parent=MS.gene040643
chr1.2 exon 33343694 33344010 33343694 ID=MS.gene040643.t1.exon1;Parent=MS.gene040643.t1
chr1.2 CDS 33343694 33344010 33343694 ID=cds.MS.gene040643.t1;Parent=MS.gene040643.t1
chr1.2 exon 33342086 33342257 33342086 ID=MS.gene040643.t1.exon2;Parent=MS.gene040643.t1
chr1.2 CDS 33342086 33342257 33342086 ID=cds.MS.gene040643.t1;Parent=MS.gene040643.t1
chr1.2 exon 33341825 33342006 33341825 ID=MS.gene040643.t1.exon3;Parent=MS.gene040643.t1
chr1.2 CDS 33341825 33342006 33341825 ID=cds.MS.gene040643.t1;Parent=MS.gene040643.t1
chr1.2 exon 33341323 33341481 33341323 ID=MS.gene040643.t1.exon4;Parent=MS.gene040643.t1
chr1.2 CDS 33341323 33341481 33341323 ID=cds.MS.gene040643.t1;Parent=MS.gene040643.t1
chr1.2 exon 33340790 33341087 33340790 ID=MS.gene040643.t1.exon5;Parent=MS.gene040643.t1
chr1.2 CDS 33340790 33341087 33340790 ID=cds.MS.gene040643.t1;Parent=MS.gene040643.t1
Gene Sequence

>MS.gene040643

ATGGCAACACCAATGACTCATTTGCTTTCTTTTTTCTTGCTGTTCAGCATCTTCCACTGCACATTATCTCTTCTGGACCCACAAGATGATGTTGGGTTTCTAGTTGAACCTGATGATGTTAACATTGTCCAAAACAATAGAGAATCTAGCAAAAGATGTGACTTTTCTGTTGGAAAATGGGTTTATGATGAATCTTACCCTCTTTATGATCCTAACTGCCCTTATCTTAGTACTGCAGTTACTTGTCAAAAGAATGGGAGGCCAGATTTTGATTATGAGAAGTGGAAGTGGAAGCCTAGTGGTTGTTCTATCCCAAGGTCTGTTCACATTGCCACCTTTTTGTTTTCTTCTATACACTCTCATTTACTCAATATTTATTTATTTATTATTTCTGTTTTATATTTAGTATATATGTGATCCATCATGACACATGTATTTACTTGGTCAAAATTGGTCTGTGGCCAAAAGGGTGTAAAGTTAGAGGGCTGATTTGTTATTGTTTTTTATAGGGGGCCAAAATCACAAGATTGTGATACTTAGGGGGCCAAAACTGCAATTAAACCTATGATCCATCATTGTTATGCACATGAAATAAAATAAAAATTTGAGAAATGTTAACGTGTCCTCTCATGGATTTGTTAATAAGTACAAAATATAAATTTTATCTAAGGATGATGCATTCAATTAAATATAACTTTAAAAAACAATTTTTTTTTTTTACCTAAAATTTGTCAATTGTTTCCTTTTTTTAATCTTTAACAAGTGCCCTCGGAGCACTTAGAATTTCAATAGTAGTCCTGCATCCATTTAATTGATAAACAAAGTTTACTAATCAAACATAGCCTATTGACGTTGATTTGAGATCTTAGAGTGTGCTTTTCTTAAGGTCTTAGTTTGATTCCTTCTGTATCAATTTCGGTGGACTAGTTATATAGAGATTTGATTTGACTTTAAATGAGCCCCCACAAGTGAACAATGAGATTAGATCTCTCGAATTAGTCGGTCTTTAGGCCGGATATTGAGTTCTAAAAAAAACATGGTTGGACTTTTTAGTTAATTAATTTGAGTATTTACCTTGACACAAGTAGTCATAGTATATTCTAGATTTTTTAGGGGAATATATTCTAGATTTATCATTCAAATTTGTGGTACTTTTGTCACTTTTTTATTTTTGTCAATGCTTGTTTTTTATCAACTCTGAGTAGGGGAGTGGAATGTGGGATGTTACTATGTCATTCCAACCCTCCATTGATCTCAGATTTTAATCCCATCTCCTATATGCATAACTTCCATAGTCATGGTTTTTGTGTTATTGTAATTTGTACGGATATGGTAAGCTTGTGGATGATGAGTTTTTGAATGGAGTAGATGATCTAATTCATCCCTGAAATTGTAAGTGTTAGTCATTTTAGTCCCCAAATTTCATGAAAAGGCAACTATGTCTGCTAAGTTGTATGAGCTAGACGTCACATCATTGCGAAGTCACAACTCACAAGGACCATTTTGGCTGAAAATATTTGTGGATTCTTATTAATGTGGTTAAATTGAAGAAAGACTAATTTTCTAGCAATTAAACCACCTTCCATTTTACTTATTTTAAGAACTATAAATGACTGATGTTCACGATTTCATGACTTAAGCGACCGACATTTACAATTTCAAGAACCAAATTTGTATTTAAGTAAATGAATTTCAACACTTTGGTATGAATAAACTCCTGCTTGTAAACTTAACTAGCTTATTACCTCTGTAGGTTTGATGCACTAAGGTTTCTTGGAAAAATGAGAAGGAAGAGAATCATGTTGGTGGGTGATTCAATAATGAGAAATCAGTGGGAATCTTTAGTATGCTTAGTACAAGGTGTAATACCAACTGGTAGAAAAAGGGTGACCTACCATGGACCTGCAATGGCCTTCCATTCAATGGTAATGAAGACATTCATCAAACACATTTTCTTTTCTTCCTTCCTTTGTAAATTATTGACATCACAGTTTTCTCTGGTAGGATTTTGAGACATCAATTGAGTTTTTATGGGCACCATTGTTAGTTGAACTGAAGAAAAGTCCAGAAAACAAGAGAGTCTTACATTTGGATTTGATTGAGGAGAATGCAAAGTATTGGAGAGGAGTTGATATCCTTGTATTTGATTCAGCTCAGTGGTGGACTCACTCCGATAAAACAAGCTCGTAAGGAAGATTCTTCATCAATTAATATTCCACCTTCTCTTCACATCGTTCTATTTTCTCAATTTCCCTTGGGCCAAACTACATAATTCAGTGTTTATAACATAAGTGTTTATCATTTAAGTGCTTATGTAAAAGATATTTTAATAACAAAAATTAAGTCAATTTTTTTATTTTTTTTTATATAAGTTGTTTTCGTTAACTATCTTAGAAAGCTTATGGGAATAAGCCAAAATCAGCTTATGGACATGGGATAAGTTGCGTCGATTAGCTTTCTAAAAGAGGCTTAATCTTAAATTTTGAATAAATGCTTCACATTTATATGCTTCTTTCTTGACTAATACAGGTGGGATTACTACAAGGAGGGAAATAATCTGATAAAAAACATGACTCTTATGGTTGCTTATCAAAAAGGACTGAGTACATGGGCAAGGTGGGTGGATCTAAATCTAAACCCTCAAAAAACCAAAGTCATTTTCAGAAGCATGTCACCTAGGCATAACAGGTACTATATACTTTTATATGCCTAAAACTTGTTTATCTTTGTCTATACAATAACGACCAAGTCTTATATCTCACTAAGTGAAGTTGATTACATGGATCAAACAACACCATAATATTTTATCGGAAATCATGTTTATAGCCAAACCAGCCATTGAGCTTCTAAATCTTAACAGTTTTTCGCCTATAGTTTTGTTTTTGGCATTTCATTGTCTCTAGTGATTGGATTTTTTATGTAGGGAAAATGGTTGGAAATGTGACAATCAGAAACAACCTCTACCATTTTTCAGCCTGCAACAACATGCTCCTGAACCTTTAGTTGTTCTCCAAGGAGTGCTGAAGAAAATGAGGTTTCCGGTATACCTGCAAGATATTACGACAATGACCGCCTTACGCAGAGATGGACATCCTTCAGTGTATAGGAAGGCAACCAGCCAAGATGAGAAACAAAAAATAGGCCATTCATCTGATTGCAGTCATTGGTGCCTCCCTGGTGTGCCTGATATATGGAATGAGATGTTGAGTGCGCTGCTTTGA
Protein sequence

>MS.gene040643.t1

MATPMTHLLSFFLLFSIFHCTLSLLDPQDDVGFLVEPDDVNIVQNNRESSKRCDFSVGKWVYDESYPLYDPNCPYLSTAVTCQKNGRPDFDYEKWKWKPSGCSIPRFDALRFLGKMRRKRIMLVGDSIMRNQWESLVCLVQGVIPTGRKRVTYHGPAMAFHSMDFETSIEFLWAPLLVELKKSPENKRVLHLDLIEENAKYWRGVDILVFDSAQWWTHSDKTSSWDYYKEGNNLIKNMTLMVAYQKGLSTWARWVDLNLNPQKTKVIFRSMSPRHNRENGWKCDNQKQPLPFFSLQQHAPEPLVVLQGVLKKMRFPVYLQDITTMTALRRDGHPSVYRKATSQDEKQKIGHSSDCSHWCLPGVPDIWNEMLSALL