AlfalfaGEDB | MS.gene04096

M. sativa cultivar XinJiangDaYe / MS.gene04096

KEGG: K12898
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00076.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04096.t1	XP_003600475.1	99.2	244	2	0	1	244	16	259	2.90E-135	491.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04096.t1	Q8C5Q4	33.2	226	108	4	43	229	250	471	9.0e-24	112.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04096.t1	G7J263	99.2	244	2	0	1	244	16	259	2.1e-135	491.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene04096	MS.gene006028	PPI
MS.gene04096	MS.gene041727	PPI
MS.gene33973	MS.gene04096	PPI
MS.gene60814	MS.gene04096	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04096.t1	MTR_3g061590	99.180	244	2	0	1	244	16	259	3.25e-180	494
MS.gene04096.t1	MTR_8g073580	50.199	251	113	4	1	244	44	289	4.70e-72	222
MS.gene04096.t1	MTR_8g073580	52.632	209	88	3	43	244	38	242	2.91e-71	218

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04096.t1	AT3G20890	61.786	280	69	4	1	244	15	292	3.41e-107	311
MS.gene04096.t1	AT3G20890	59.113	203	50	1	75	244	27	229	6.47e-80	239
MS.gene04096.t1	AT5G66010	48.729	236	108	5	1	232	16	242	3.91e-71	218
MS.gene04096.t1	AT5G66010	47.260	146	72	2	91	232	1	145	9.71e-43	142

Find 69 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGCGAAGGCTTCTGTGTTT+TGG	0.180490	3.2:-52975909	MS.gene04096:CDS
GACGCGGGATCTTCATTCTA+TGG	0.326213	3.2:-52976121	MS.gene04096:CDS
TCATCCTTATTAGCAGAAAA+TGG	0.345506	3.2:+52975659	None:intergenic
AATTCAGAGGGAAGACCATC+TGG	0.348327	3.2:-52975578	MS.gene04096:CDS
AGATGGATTGTACATATAAC+TGG	0.382208	3.2:+52976087	None:intergenic
ACTTTGCTCTTCAAAGAAAC+AGG	0.406141	3.2:-52975868	MS.gene04096:CDS
TGCTAATAAGGATGACATAA+TGG	0.410596	3.2:-52975651	MS.gene04096:CDS
TCTTCATTCTATGGTTCCCC+TGG	0.414721	3.2:-52976112	MS.gene04096:CDS
TCCATCTCACCATGCGTTGA+AGG	0.422533	3.2:+52975461	None:intergenic
CTACAAGGCAATTGCAAATG+AGG	0.431623	3.2:-52975792	MS.gene04096:CDS
CGCAGTCGAACAACAGGAAA+TGG	0.442842	3.2:+52976031	None:intergenic
AGACCGCGCAGTCGAACAAC+AGG	0.447152	3.2:+52976025	None:intergenic
CTCCATCATATGATTTAGCT+CGG	0.453352	3.2:+52975729	None:intergenic
ACCATCTGGGGAGGCTTATG+TGG	0.470749	3.2:-52975564	MS.gene04096:CDS
CTTCAAGGAGTATGTGTTGT+CGG	0.500765	3.2:-52975624	MS.gene04096:CDS
CACAGGAGTGTTGCGACTGA+GGG	0.505697	3.2:-52975687	MS.gene04096:CDS
TCCATCATATGATTTAGCTC+GGG	0.508153	3.2:+52975730	None:intergenic
ATGTACAATCCATCTCCATA+TGG	0.508179	3.2:-52976079	MS.gene04096:CDS
CTCGGGGAGCACTACGACGA+GGG	0.508597	3.2:+52975747	None:intergenic
ACAGGAAATGGTGGAGGAGG+AGG	0.509420	3.2:+52976043	None:intergenic
ATCAGTTTCAGTGCAATCAA+AGG	0.523371	3.2:+52976000	None:intergenic
CCATCTCACCATGCGTTGAA+GGG	0.532489	3.2:+52975462	None:intergenic
TGACATAATGGAGTTCTTCA+AGG	0.535855	3.2:-52975639	MS.gene04096:CDS
CCCCGAGCTAAATCATATGA+TGG	0.536873	3.2:-52975731	MS.gene04096:CDS
CCCTTCAACGCATGGTGAGA+TGG	0.538750	3.2:-52975462	MS.gene04096:CDS
GATGTGGCTGAGTTCTTCCA+TGG	0.538968	3.2:-52975980	MS.gene04096:CDS
GCAAAGGATAGAATGACGCT+TGG	0.544787	3.2:-52975506	MS.gene04096:CDS
CGTCATTCTATCCTTTGCCA+TGG	0.545218	3.2:+52975511	None:intergenic
TCAGTTTCAGTGCAATCAAA+GGG	0.547953	3.2:+52976001	None:intergenic
CAAAGGATAGAATGACGCTT+GGG	0.551160	3.2:-52975505	MS.gene04096:CDS
GGTTGGCTGACATATCCATA+TGG	0.556296	3.2:+52976064	None:intergenic
CAAAGAAACAGGCAGAACAT+AGG	0.560049	3.2:-52975857	MS.gene04096:CDS
CTGACATATCCATATGGAGA+TGG	0.564910	3.2:+52976070	None:intergenic
ATTCAGAGGGAAGACCATCT+GGG	0.571117	3.2:-52975577	MS.gene04096:CDS
TGTACATATAACTGGAACCA+GGG	0.571227	3.2:+52976095	None:intergenic
AGAGCAAAGTCAACTTGAAG+AGG	0.573046	3.2:+52975881	None:intergenic
ACAACAGGAAATGGTGGAGG+AGG	0.573282	3.2:+52976040	None:intergenic
TCCACATAAGCCTCCCCAGA+TGG	0.579302	3.2:+52975563	None:intergenic
GAGCTATTCCCTTCAACGCA+TGG	0.586026	3.2:-52975470	MS.gene04096:CDS
TGATGATTCAAAAGCAGCCA+TGG	0.593984	3.2:-52975528	MS.gene04096:CDS
ACACAGGAGTGTTGCGACTG+AGG	0.596079	3.2:-52975688	MS.gene04096:CDS
TTCAAAAGCAGCCATGGCAA+AGG	0.597698	3.2:-52975522	MS.gene04096:CDS
CCACATTGTAATGAATTCAG+AGG	0.601918	3.2:-52975591	MS.gene04096:CDS
TGATTGCACTGAAACTGATG+TGG	0.603910	3.2:-52975996	MS.gene04096:CDS
CGAACAACAGGAAATGGTGG+AGG	0.604157	3.2:+52976037	None:intergenic
GAAATGGTGGAGGAGGAGGT+TGG	0.608351	3.2:+52976047	None:intergenic
ATGGAAGATGCAATTTCAAG+AGG	0.609038	3.2:-52975443	MS.gene04096:CDS
CACATTGTAATGAATTCAGA+GGG	0.613126	3.2:-52975590	MS.gene04096:CDS
CCATCATATGATTTAGCTCG+GGG	0.614326	3.2:+52975731	None:intergenic
ACGTCAGCGGACAGTTGACG+CGG	0.625189	3.2:-52976137	MS.gene04096:CDS
TCGGGGAGCACTACGACGAG+GGG	0.629479	3.2:+52975748	None:intergenic
AAAGATTCAGCTGAGCACAC+AGG	0.630687	3.2:-52975704	MS.gene04096:CDS
TTTCCTGTTGTTCGACTGCG+CGG	0.636494	3.2:-52976028	MS.gene04096:CDS
TAAGAGGCAGGAATACTACA+AGG	0.640162	3.2:-52975807	MS.gene04096:CDS
CCTCTGAATTCATTACAATG+TGG	0.641568	3.2:+52975591	None:intergenic
TTCAGAGGGAAGACCATCTG+GGG	0.646058	3.2:-52975576	MS.gene04096:CDS
CAAGGAGTATGTGTTGTCGG+AGG	0.654683	3.2:-52975621	MS.gene04096:CDS
CGTCAGCGGACAGTTGACGC+GGG	0.660169	3.2:-52976136	MS.gene04096:CDS
CGAGCTAAATCATATGATGG+AGG	0.660288	3.2:-52975728	MS.gene04096:CDS
TTGTACATATAACTGGAACC+AGG	0.660817	3.2:+52976094	None:intergenic
CATCAACTATGTCTAGACCA+TGG	0.660852	3.2:+52975963	None:intergenic
AGAGGGAAGACCATCTGGGG+AGG	0.662119	3.2:-52975573	MS.gene04096:CDS
GCTCGGGGAGCACTACGACG+AGG	0.665801	3.2:+52975746	None:intergenic
AGTCGAACAACAGGAAATGG+TGG	0.675557	3.2:+52976034	None:intergenic
ACAGGAGTGTTGCGACTGAG+GGG	0.684610	3.2:-52975686	MS.gene04096:CDS
AATGAGGTTTCAGATGCTCG+AGG	0.692222	3.2:-52975776	MS.gene04096:CDS
AAATGGCTTCAAAACGTCAG+CGG	0.695070	3.2:-52976150	MS.gene04096:CDS
GAGGTTTCAGATGCTCGAGG+CGG	0.695093	3.2:-52975773	MS.gene04096:CDS
GTACATATAACTGGAACCAG+GGG	0.705619	3.2:+52976096	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTACCATTTTCTGCTAATA+AGG	-	chr3.2:52975921-52975940	MS.gene04096:CDS	25.0%
	CACATTGTAATGAATTCAGA+GGG	-	chr3.2:52975994-52976013	MS.gene04096:CDS	30.0%
	TCATCCTTATTAGCAGAAAA+TGG	+	chr3.2:52975928-52975947	None:intergenic	30.0%
	TGCTAATAAGGATGACATAA+TGG	-	chr3.2:52975933-52975952	MS.gene04096:CDS	30.0%
!	GATGTTCTTTTTGTTCACAA+AGG	-	chr3.2:52975640-52975659	MS.gene04096:CDS	30.0%
!	TTGAAGTTTTCAGAAGTAAG+AGG	-	chr3.2:52975761-52975780	MS.gene04096:CDS	30.0%
!!	AGATGGATTGTACATATAAC+TGG	+	chr3.2:52975500-52975519	None:intergenic	30.0%
	ACTTTGCTCTTCAAAGAAAC+AGG	-	chr3.2:52975716-52975735	MS.gene04096:CDS	35.0%
	ATCAGTTTCAGTGCAATCAA+AGG	+	chr3.2:52975587-52975606	None:intergenic	35.0%
	ATGGAAGATGCAATTTCAAG+AGG	-	chr3.2:52976141-52976160	MS.gene04096:CDS	35.0%
	ATGTACAATCCATCTCCATA+TGG	-	chr3.2:52975505-52975524	MS.gene04096:CDS	35.0%
	CCACATTGTAATGAATTCAG+AGG	-	chr3.2:52975993-52976012	MS.gene04096:CDS	35.0%
	CCTCTGAATTCATTACAATG+TGG	+	chr3.2:52975996-52976015	None:intergenic	35.0%
	CTCCATCATATGATTTAGCT+CGG	+	chr3.2:52975858-52975877	None:intergenic	35.0%
	TCAGTTTCAGTGCAATCAAA+GGG	+	chr3.2:52975586-52975605	None:intergenic	35.0%
	TGTACATATAACTGGAACCA+GGG	+	chr3.2:52975492-52975511	None:intergenic	35.0%
	TTGTACATATAACTGGAACC+AGG	+	chr3.2:52975493-52975512	None:intergenic	35.0%
!	GTTCTTTTTGTTCACAAAGG+TGG	-	chr3.2:52975643-52975662	MS.gene04096:CDS	35.0%
!	TCCATCATATGATTTAGCTC+GGG	+	chr3.2:52975857-52975876	None:intergenic	35.0%
!	TGACATAATGGAGTTCTTCA+AGG	-	chr3.2:52975945-52975964	MS.gene04096:CDS	35.0%
	AGAGCAAAGTCAACTTGAAG+AGG	+	chr3.2:52975706-52975725	None:intergenic	40.0%
	CAAAGAAACAGGCAGAACAT+AGG	-	chr3.2:52975727-52975746	MS.gene04096:CDS	40.0%
	CAAAGGATAGAATGACGCTT+GGG	-	chr3.2:52976079-52976098	MS.gene04096:CDS	40.0%
	CATCAACTATGTCTAGACCA+TGG	+	chr3.2:52975624-52975643	None:intergenic	40.0%
	CGAGCTAAATCATATGATGG+AGG	-	chr3.2:52975856-52975875	MS.gene04096:CDS	40.0%
	CTACAAGGCAATTGCAAATG+AGG	-	chr3.2:52975792-52975811	MS.gene04096:CDS	40.0%
	CTGACATATCCATATGGAGA+TGG	+	chr3.2:52975517-52975536	None:intergenic	40.0%
	GTACATATAACTGGAACCAG+GGG	+	chr3.2:52975491-52975510	None:intergenic	40.0%
	TAAGAGGCAGGAATACTACA+AGG	-	chr3.2:52975777-52975796	MS.gene04096:CDS	40.0%
	TGATGATTCAAAAGCAGCCA+TGG	-	chr3.2:52976056-52976075	MS.gene04096:CDS	40.0%
	TGATTGCACTGAAACTGATG+TGG	-	chr3.2:52975588-52975607	MS.gene04096:CDS	40.0%
!	AGTTTTCAGAAGTAAGAGGC+AGG	-	chr3.2:52975765-52975784	MS.gene04096:CDS	40.0%
!	CCATCATATGATTTAGCTCG+GGG	+	chr3.2:52975856-52975875	None:intergenic	40.0%
!	CTTCAAGGAGTATGTGTTGT+CGG	-	chr3.2:52975960-52975979	MS.gene04096:CDS	40.0%
	AAAGATTCAGCTGAGCACAC+AGG	-	chr3.2:52975880-52975899	MS.gene04096:CDS	45.0%
	AATGAGGTTTCAGATGCTCG+AGG	-	chr3.2:52975808-52975827	MS.gene04096:CDS	45.0%
	AATTCAGAGGGAAGACCATC+TGG	-	chr3.2:52976006-52976025	MS.gene04096:CDS	45.0%
	AGTCGAACAACAGGAAATGG+TGG	+	chr3.2:52975553-52975572	None:intergenic	45.0%
	ATTCAGAGGGAAGACCATCT+GGG	-	chr3.2:52976007-52976026	MS.gene04096:CDS	45.0%
	CCCCGAGCTAAATCATATGA+TGG	-	chr3.2:52975853-52975872	MS.gene04096:CDS	45.0%
	CGTCATTCTATCCTTTGCCA+TGG	+	chr3.2:52976076-52976095	None:intergenic	45.0%
	GCAAAGGATAGAATGACGCT+TGG	-	chr3.2:52976078-52976097	MS.gene04096:CDS	45.0%
	TCTTCATTCTATGGTTCCCC+TGG	-	chr3.2:52975472-52975491	MS.gene04096:CDS	45.0%
	TTCAAAAGCAGCCATGGCAA+AGG	-	chr3.2:52976062-52976081	MS.gene04096:CDS	45.0%
!	CACAAAGGTGGCAAGTTTTC+TGG	-	chr3.2:52975655-52975674	MS.gene04096:CDS	45.0%
!	GGTTGGCTGACATATCCATA+TGG	+	chr3.2:52975523-52975542	None:intergenic	45.0%
	CCATCTCACCATGCGTTGAA+GGG	+	chr3.2:52976125-52976144	None:intergenic	50.0%
	CGAACAACAGGAAATGGTGG+AGG	+	chr3.2:52975550-52975569	None:intergenic	50.0%
	CGCAGTCGAACAACAGGAAA+TGG	+	chr3.2:52975556-52975575	None:intergenic	50.0%
	GACGCGGGATCTTCATTCTA+TGG	-	chr3.2:52975463-52975482	MS.gene04096:CDS	50.0%
	GAGCTATTCCCTTCAACGCA+TGG	-	chr3.2:52976114-52976133	MS.gene04096:CDS	50.0%
	GATGTGGCTGAGTTCTTCCA+TGG	-	chr3.2:52975604-52975623	MS.gene04096:CDS	50.0%
	TCCATCTCACCATGCGTTGA+AGG	+	chr3.2:52976126-52976145	None:intergenic	50.0%
	TTCAGAGGGAAGACCATCTG+GGG	-	chr3.2:52976008-52976027	MS.gene04096:CDS	50.0%
	TTTCCTGTTGTTCGACTGCG+CGG	-	chr3.2:52975556-52975575	MS.gene04096:CDS	50.0%
!	ACAACAGGAAATGGTGGAGG+AGG	+	chr3.2:52975547-52975566	None:intergenic	50.0%
!	CAAGGAGTATGTGTTGTCGG+AGG	-	chr3.2:52975963-52975982	MS.gene04096:CDS	50.0%
!	GGCGAAGGCTTCTGTGTTTT+GGG	-	chr3.2:52975676-52975695	MS.gene04096:CDS	50.0%
!	TGGCGAAGGCTTCTGTGTTT+TGG	-	chr3.2:52975675-52975694	MS.gene04096:CDS	50.0%
	ACCATCTGGGGAGGCTTATG+TGG	-	chr3.2:52976020-52976039	MS.gene04096:CDS	55.0%
	CCCTTCAACGCATGGTGAGA+TGG	-	chr3.2:52976122-52976141	MS.gene04096:CDS	55.0%
	GAGGTTTCAGATGCTCGAGG+CGG	-	chr3.2:52975811-52975830	MS.gene04096:CDS	55.0%
	TCCACATAAGCCTCCCCAGA+TGG	+	chr3.2:52976024-52976043	None:intergenic	55.0%
!	ACAGGAAATGGTGGAGGAGG+AGG	+	chr3.2:52975544-52975563	None:intergenic	55.0%
!	GAAATGGTGGAGGAGGAGGT+TGG	+	chr3.2:52975540-52975559	None:intergenic	55.0%
!!	ACACAGGAGTGTTGCGACTG+AGG	-	chr3.2:52975896-52975915	MS.gene04096:CDS	55.0%
!!	ACAGGAGTGTTGCGACTGAG+GGG	-	chr3.2:52975898-52975917	MS.gene04096:CDS	55.0%
!!	CACAGGAGTGTTGCGACTGA+GGG	-	chr3.2:52975897-52975916	MS.gene04096:CDS	55.0%
!!	GGTGGCAAGTTTTCTGGCGA+AGG	-	chr3.2:52975661-52975680	MS.gene04096:CDS	55.0%
	ACGTCAGCGGACAGTTGACG+CGG	-	chr3.2:52975447-52975466	MS.gene04096:CDS	60.0%
	AGACCGCGCAGTCGAACAAC+AGG	+	chr3.2:52975562-52975581	None:intergenic	60.0%
	AGAGGGAAGACCATCTGGGG+AGG	-	chr3.2:52976011-52976030	MS.gene04096:CDS	60.0%
	CGTCAGCGGACAGTTGACGC+GGG	-	chr3.2:52975448-52975467	MS.gene04096:CDS	65.0%
!!	CTCGGGGAGCACTACGACGA+GGG	+	chr3.2:52975840-52975859	None:intergenic	65.0%
!!	TCGGGGAGCACTACGACGAG+GGG	+	chr3.2:52975839-52975858	None:intergenic	65.0%
!!	GCTCGGGGAGCACTACGACG+AGG	+	chr3.2:52975841-52975860	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	52975436	52976170	52975436	ID=MS.gene04096
chr3.2	mRNA	52975436	52976170	52975436	ID=MS.gene04096.t1;Parent=MS.gene04096
chr3.2	exon	52975436	52976170	52975436	ID=MS.gene04096.t1.exon1;Parent=MS.gene04096.t1
chr3.2	CDS	52975436	52976170	52975436	ID=cds.MS.gene04096.t1;Parent=MS.gene04096.t1

Gene Sequence

>MS.gene04096

ATGGCTTCAAAACGTCAGCGGACAGTTGACGCGGGATCTTCATTCTATGGTTCCCCTGGTTCCAGTTATATGTACAATCCATCTCCATATGGATATGTCAGCCAACCTCCTCCTCCACCATTTCCTGTTGTTCGACTGCGCGGTCTTCCCTTTGATTGCACTGAAACTGATGTGGCTGAGTTCTTCCATGGTCTAGACATAGTTGATGTTCTTTTTGTTCACAAAGGTGGCAAGTTTTCTGGCGAAGGCTTCTGTGTTTTGGGATATCCTCTTCAAGTTGACTTTGCTCTTCAAAGAAACAGGCAGAACATAGGTAGAAGATATGTTGAAGTTTTCAGAAGTAAGAGGCAGGAATACTACAAGGCAATTGCAAATGAGGTTTCAGATGCTCGAGGCGGTTCCCCTCGTCGTAGTGCTCCCCGAGCTAAATCATATGATGGAGGAAAAGATTCAGCTGAGCACACAGGAGTGTTGCGACTGAGGGGATTACCATTTTCTGCTAATAAGGATGACATAATGGAGTTCTTCAAGGAGTATGTGTTGTCGGAGGATTCGATCCACATTGTAATGAATTCAGAGGGAAGACCATCTGGGGAGGCTTATGTGGAATTTGAAAATGCTGATGATTCAAAAGCAGCCATGGCAAAGGATAGAATGACGCTTGGGAGTCGCTATATAGAGCTATTCCCTTCAACGCATGGTGAGATGGAAGATGCAATTTCAAGAGGACGATGA

Protein sequence

>MS.gene04096.t1

MASKRQRTVDAGSSFYGSPGSSYMYNPSPYGYVSQPPPPPFPVVRLRGLPFDCTETDVAEFFHGLDIVDVLFVHKGGKFSGEGFCVLGYPLQVDFALQRNRQNIGRRYVEVFRSKRQEYYKAIANEVSDARGGSPRRSAPRAKSYDGGKDSAEHTGVLRLRGLPFSANKDDIMEFFKEYVLSEDSIHIVMNSEGRPSGEAYVEFENADDSKAAMAKDRMTLGSRYIELFPSTHGEMEDAISRGR