AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60814

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60814.t1 MTR_1g093095 41.447 152 83 6 239 387 107 255 2.19e-26 108
MS.gene60814.t1 MTR_1g112270 36.076 158 88 5 224 381 63 207 1.05e-24 108
MS.gene60814.t1 MTR_1g112270 35.762 151 85 4 231 381 58 196 1.94e-24 107
MS.gene60814.t1 MTR_4g059870 34.868 152 86 4 231 381 52 191 2.92e-24 106
MS.gene60814.t1 MTR_8g017210 35.099 151 86 3 231 381 76 214 7.65e-24 105
MS.gene60814.t1 MTR_2g099990 34.503 171 95 5 215 381 41 198 1.81e-23 103
MS.gene60814.t1 MTR_1g094115 33.775 151 88 4 231 381 61 199 6.31e-23 102
MS.gene60814.t1 MTR_2g093960 35.099 151 87 4 231 381 46 185 6.91e-23 101
MS.gene60814.t1 MTR_1g016010 35.099 151 87 3 231 381 55 194 3.10e-22 99.8
MS.gene60814.t1 MTR_2g090745 31.765 170 101 4 215 381 2 159 3.88e-21 96.3
MS.gene60814.t1 MTR_2g099990 36.170 141 78 3 241 381 1 129 3.97e-21 95.9
MS.gene60814.t1 MTR_1g094115 35.000 140 79 4 242 381 55 182 8.13e-21 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60814.t1 AT1G34370 55.534 515 191 9 1 496 1 496 1.72e-178 511
MS.gene60814.t1 AT1G34370 55.534 515 191 9 1 496 1 496 1.72e-178 511
MS.gene60814.t1 AT1G34370 71.528 288 74 3 214 496 2 286 6.73e-142 409
MS.gene60814.t1 AT5G22890 40.517 348 179 10 64 401 39 368 9.01e-75 240
MS.gene60814.t1 AT1G51220 37.423 163 92 5 228 387 160 315 8.05e-26 108
MS.gene60814.t1 AT1G34790 40.645 155 79 7 238 387 136 282 1.32e-25 107
MS.gene60814.t1 AT1G13290 39.535 172 91 8 220 386 76 239 2.92e-25 106
MS.gene60814.t1 AT3G20880 37.821 156 87 5 240 392 250 398 9.36e-25 106
MS.gene60814.t1 AT1G08290 40.000 150 81 5 241 387 175 318 1.50e-24 104
MS.gene60814.t1 AT3G45260 35.762 151 86 3 231 381 53 192 2.74e-24 105
MS.gene60814.t1 AT3G45260 35.762 151 86 3 231 381 53 192 2.74e-24 105
MS.gene60814.t1 AT5G44160 34.437 151 87 3 231 381 51 189 2.99e-24 105
MS.gene60814.t1 AT5G44160 34.437 151 87 3 231 381 12 150 3.73e-24 105
MS.gene60814.t1 AT5G66730 34.437 151 87 3 231 381 46 184 3.84e-24 105
MS.gene60814.t1 AT3G57670 33.498 203 119 6 215 409 181 375 4.10e-24 104
MS.gene60814.t1 AT1G03840 34.545 165 93 5 219 381 40 191 7.24e-24 105
MS.gene60814.t1 AT1G03840 34.437 151 87 3 231 381 55 193 1.26e-23 104
MS.gene60814.t1 AT5G60470 35.135 148 85 3 231 378 58 194 1.43e-23 103
MS.gene60814.t1 AT5G60470 35.135 148 85 3 231 378 49 185 1.43e-23 103
MS.gene60814.t1 AT5G60470 35.135 148 85 3 231 378 54 190 1.50e-23 103
MS.gene60814.t1 AT5G03150 35.294 153 84 4 231 381 67 206 1.87e-23 103
MS.gene60814.t1 AT1G14580 33.775 151 88 3 231 381 67 205 2.77e-23 102
MS.gene60814.t1 AT1G14580 33.775 151 88 3 231 381 67 205 2.77e-23 102
MS.gene60814.t1 AT1G14580 33.775 151 88 3 231 381 81 219 3.35e-23 102
MS.gene60814.t1 AT2G02080 33.775 151 88 3 231 381 68 206 6.22e-23 102
MS.gene60814.t1 AT2G02080 33.775 151 88 3 231 381 68 206 6.22e-23 102
MS.gene60814.t1 AT2G02080 33.775 151 88 3 231 381 68 206 6.22e-23 102
MS.gene60814.t1 AT2G02080 33.775 151 88 3 231 381 68 206 6.22e-23 102
MS.gene60814.t1 AT1G55110 33.775 151 89 3 231 381 77 216 6.39e-23 101
MS.gene60814.t1 AT1G55110 33.775 151 89 3 231 381 77 216 6.39e-23 101
MS.gene60814.t1 AT1G55110 33.775 151 89 3 231 381 77 216 6.39e-23 101
MS.gene60814.t1 AT3G50700 35.762 151 85 3 231 381 48 186 8.32e-23 101
MS.gene60814.t1 AT2G02070 33.113 151 89 3 231 381 66 204 1.13e-22 102
MS.gene60814.t1 AT2G02070 33.113 151 89 3 231 381 66 204 1.13e-22 102
MS.gene60814.t1 AT3G13810 34.437 151 88 3 231 381 79 218 1.26e-22 101
MS.gene60814.t1 AT3G13810 35.220 159 91 4 223 381 78 224 1.35e-22 101
MS.gene60814.t1 AT3G13810 35.220 159 91 4 223 381 64 210 1.47e-22 101
MS.gene60814.t1 AT3G13810 34.437 151 88 3 231 381 84 223 1.50e-22 101
MS.gene60814.t1 AT3G13810 34.437 151 88 3 231 381 84 223 1.50e-22 101
MS.gene60814.t1 AT3G13810 34.437 151 88 3 231 381 65 204 1.54e-22 100
MS.gene60814.t1 AT4G02670 35.099 151 87 3 231 381 69 208 1.63e-21 97.1
MS.gene60814.t1 AT4G02670 35.099 151 87 3 231 381 67 206 1.66e-21 97.1
MS.gene60814.t1 AT2G02080 34.752 141 80 3 241 381 1 129 8.35e-21 95.1
MS.gene60814.t1 AT2G02080 34.752 141 80 3 241 381 1 129 8.35e-21 95.1
MS.gene60814.t1 AT1G25250 33.333 159 95 5 221 378 14 162 9.45e-18 85.1
MS.gene60814.t1 AT1G68130 34.899 149 85 6 231 378 55 192 3.18e-17 84.3
MS.gene60814.t1 AT1G25250 34.228 149 87 5 231 378 47 185 3.99e-17 83.6
MS.gene60814.t1 AT2G01940 32.886 149 88 5 231 378 58 195 6.99e-17 83.6
MS.gene60814.t1 AT2G01940 32.847 137 80 5 231 366 58 183 2.91e-14 75.5

Find 114 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATTCCCACACTGTAAAA+AGG 0.196818 1.2:-72355435 None:intergenic
TCAAATCCTTCAAGTGAAAA+TGG 0.258615 1.2:+72356596 MS.gene60814:CDS
TGCACCAATTCACAATTATT+AGG 0.289796 1.2:-72355921 None:intergenic
AACTTTGAAGGCTGTAACTT+TGG 0.299493 1.2:+72356683 MS.gene60814:CDS
TCCTTGGCTCCCACTGGGAA+TGG 0.315241 1.2:+72355381 MS.gene60814:CDS
AACACTGTGGCAAGGATAAA+TGG 0.316031 1.2:+72356393 MS.gene60814:CDS
GCAAAACCACACAAAGAAAC+TGG 0.316849 1.2:+72356167 MS.gene60814:CDS
TCGAACTCAGTCTGTGATTC+CGG 0.317053 1.2:+72355488 MS.gene60814:CDS
GGAAGCATGAACTTCTGTTT+TGG 0.324815 1.2:+72356572 MS.gene60814:CDS
TCCTCTTCCTTGGCTCCCAC+TGG 0.331174 1.2:+72355375 MS.gene60814:CDS
TTTAATTATGCTCCTAAGTC+TGG 0.333799 1.2:+72356779 MS.gene60814:CDS
AACGGGATTAGCCTTCCTTT+TGG 0.342253 1.2:+72355819 MS.gene60814:CDS
CCATACACCGGCTATTCCTT+TGG 0.353696 1.2:+72356484 MS.gene60814:CDS
TTCTCACCTTTGAACTTTGA+AGG 0.355262 1.2:+72356671 MS.gene60814:CDS
GGAGTCTTCTCCCTTCTGTT+AGG 0.361673 1.2:+72355748 MS.gene60814:CDS
GTTACAAATGAATTTGTCTT+CGG 0.368363 1.2:+72355404 MS.gene60814:CDS
CCTTGGCTCCCACTGGGAAT+GGG 0.390829 1.2:+72355382 MS.gene60814:CDS
TTACAAATGAATTTGTCTTC+GGG 0.398647 1.2:+72355405 MS.gene60814:CDS
GAAGCTTGTGTGCTAACAAC+TGG 0.402631 1.2:+72355346 MS.gene60814:CDS
CTGGGCTAGGTCCTCTTCCT+TGG 0.412335 1.2:+72355365 MS.gene60814:CDS
GACAGCTTAGCCAACTTAAC+GGG 0.414271 1.2:+72355802 MS.gene60814:CDS
TGAATCTTCAAACGGAGGTT+GGG 0.420262 1.2:-72356723 None:intergenic
CTGAATCTTCAAACGGAGGT+TGG 0.420596 1.2:-72356724 None:intergenic
GAATAGCCGGTGTATGGCCT+TGG 0.431520 1.2:-72356478 None:intergenic
AGTTCACGCGGTTGTTCAAA+AGG 0.437372 1.2:-72355654 None:intergenic
AAATGGAGCTGATAGCATTA+TGG 0.446519 1.2:+72356613 MS.gene60814:CDS
AAATGAGTAAACTTCAAGAC+TGG 0.451293 1.2:+72355535 MS.gene60814:CDS
AGACGAAGAAGATGTGGAAG+AGG 0.452899 1.2:+72355974 MS.gene60814:CDS
GTGGCTCGGCAGATCCTTTG+TGG 0.455339 1.2:-72356511 None:intergenic
CAATCAAATGCACAAGATCC+TGG 0.461240 1.2:-72355614 None:intergenic
CAACTTGTGCTTGTTCCAAA+AGG 0.469664 1.2:-72355834 None:intergenic
TGATATTAAGATCACTGATC+AGG 0.471924 1.2:+72355512 MS.gene60814:CDS
GTTACGCTTGCAGCCACCAT+AGG 0.472872 1.2:-72356225 None:intergenic
TTGAAGGCTGTAACTTTGGC+GGG 0.475842 1.2:+72356687 MS.gene60814:CDS
AAGTTTGAAATGAATCCAAC+TGG 0.479394 1.2:+72355324 None:intergenic
GGACAGCTTAGCCAACTTAA+CGG 0.484196 1.2:+72355801 MS.gene60814:CDS
CGATGTGACACCAGAGAAAA+CGG 0.484392 1.2:+72356539 MS.gene60814:CDS
GAATACCTCTTGATAAGCTT+CGG 0.485383 1.2:-72356197 None:intergenic
AAAGGTGAGAAATAATTGAT+AGG 0.486126 1.2:-72356659 None:intergenic
CCAAAGGAATAGCCGGTGTA+TGG 0.488982 1.2:-72356484 None:intergenic
ATTAGGTACATCCTTCTGAA+TGG 0.490088 1.2:-72355904 None:intergenic
TGTACCTAATAATTGTGAAT+TGG 0.493814 1.2:+72355917 MS.gene60814:CDS
TTCTGTACAATCTGTGGCAA+GGG 0.495568 1.2:+72356071 MS.gene60814:CDS
GTTCAGTTGATCTCTACTGC+AGG 0.497725 1.2:+72355726 MS.gene60814:CDS
CTCCCTTCTGTTAGGAACTC+CGG 0.500730 1.2:+72355756 MS.gene60814:CDS
CCTCTTCCTTGGCTCCCACT+GGG 0.502672 1.2:+72355376 MS.gene60814:CDS
TTTGAAGGCTGTAACTTTGG+CGG 0.506735 1.2:+72356686 MS.gene60814:CDS
GACGAAGAAGATGTGGAAGA+GGG 0.507980 1.2:+72355975 MS.gene60814:CDS
CATGATTCACCACCAGACTT+AGG 0.509941 1.2:-72356791 None:intergenic
TCTCTGGTGTCACATCGATC+AGG 0.512441 1.2:-72356533 None:intergenic
CCTCTTGATAAGCTTCGGTT+CGG 0.514238 1.2:-72356192 None:intergenic
GAAGGCTAATCCCGTTAAGT+TGG 0.516535 1.2:-72355812 None:intergenic
ACAAACACAAGTCCGTTGCT+TGG 0.519115 1.2:+72355780 MS.gene60814:CDS
CGAATGCACATGCGAGGCCA+TGG 0.526902 1.2:+72356116 MS.gene60814:CDS
GAGCCTTCTGAATCTTCAAA+CGG 0.528176 1.2:-72356731 None:intergenic
AAGAGGTATTCGTGTCCCTA+TGG 0.530145 1.2:+72356209 MS.gene60814:CDS
GGTTGGTTGATTTCTTTGAA+AGG 0.533908 1.2:-72355462 None:intergenic
TGTGTGCTAACAACTGGGCT+AGG 0.534923 1.2:+72355352 MS.gene60814:CDS
CCCATTCCCAGTGGGAGCCA+AGG 0.535284 1.2:-72355382 None:intergenic
GAGGGTGAGAATCTTGCTCC+TGG 0.536278 1.2:+72355993 MS.gene60814:CDS
AGAAAAGATTCATCAGCTCC+AGG 0.538362 1.2:+72355596 MS.gene60814:CDS
GTCACATCGATCAGGTGGCT+CGG 0.538841 1.2:-72356525 None:intergenic
TCGGACCCAGTTTCTTTGTG+TGG 0.543092 1.2:-72356173 None:intergenic
TTTCAATCAGTCCATTCAGA+AGG 0.546357 1.2:+72355893 MS.gene60814:CDS
TCAGTGATCTTAATATCATC+CGG 0.547189 1.2:-72355507 None:intergenic
CAACGGACTTGTGTTTGTAC+CGG 0.548811 1.2:-72355775 None:intergenic
CCCAGTGGGAGCCAAGGAAG+AGG 0.551638 1.2:-72355376 None:intergenic
TGGTCTTCCAAAGGAATAGC+CGG 0.552157 1.2:-72356491 None:intergenic
TTGTGCTTGTTCCAAAAGGA+AGG 0.553484 1.2:-72355830 None:intergenic
TTACGCTTGCAGCCACCATA+GGG 0.561037 1.2:-72356224 None:intergenic
CAGACTGAGTTCGAGATGGT+TGG 0.568725 1.2:-72355479 None:intergenic
TTACAGCCTTCAAAGTTCAA+AGG 0.572217 1.2:-72356677 None:intergenic
GTACCGGAGTTCCTAACAGA+AGG 0.573753 1.2:-72355759 None:intergenic
GATAAATGGCTTTGTTCATG+TGG 0.574687 1.2:+72356407 MS.gene60814:CDS
GATCCTTTGTGGTCTTCCAA+AGG 0.575837 1.2:-72356500 None:intergenic
TTTCTGTACAATCTGTGGCA+AGG 0.576014 1.2:+72356070 MS.gene60814:CDS
TTCAGTTGATCTCTACTGCA+GGG 0.580172 1.2:+72355727 MS.gene60814:CDS
CAAAACCACACAAAGAAACT+GGG 0.588364 1.2:+72356168 MS.gene60814:CDS
TGGTGGCTGCAAGCGTAACA+AGG 0.588657 1.2:+72356229 MS.gene60814:CDS
TTAGCACACAAGCTTCCAGT+TGG 0.591117 1.2:-72355339 None:intergenic
AATTATGCTCCTAAGTCTGG+TGG 0.594213 1.2:+72356782 MS.gene60814:CDS
AGAGAAAACGGTGCTGTGGT+AGG 0.594886 1.2:+72356551 MS.gene60814:CDS
AATGAGTAAACTTCAAGACT+GGG 0.596306 1.2:+72355536 MS.gene60814:CDS
AAGCTTATTCAACATAACAC+TGG 0.596534 1.2:-72355562 None:intergenic
GATAGCATTATGGATGTGAA+AGG 0.598382 1.2:+72356623 MS.gene60814:CDS
ACATAAAGACGAAGAAGATG+TGG 0.601216 1.2:+72355968 MS.gene60814:CDS
ACACATAAAATAGTCTTCAG+AGG 0.602166 1.2:-72356269 None:intergenic
CCTCCGTTTGAAGATTCAGA+AGG 0.608990 1.2:+72356728 MS.gene60814:CDS
AGGTATTCGTGTCCCTATGG+TGG 0.612960 1.2:+72356212 MS.gene60814:CDS
TGGCTAAGCTGTCCAAGCAA+CGG 0.613429 1.2:-72355792 None:intergenic
TTTGGAACAAGCACAAGTTG+TGG 0.616227 1.2:+72355837 MS.gene60814:CDS
TGTAAAATCTCATATGAACC+AGG 0.616349 1.2:-72356011 None:intergenic
CACCAGAGAAAACGGTGCTG+TGG 0.617127 1.2:+72356547 MS.gene60814:CDS
CTGGTGTCACATCGATCAGG+TGG 0.620999 1.2:-72356530 None:intergenic
CATGTTCTGACAATCTGTAA+CGG 0.621637 1.2:+72356810 MS.gene60814:CDS
ATTTGTAACCCATTCCCAGT+GGG 0.628478 1.2:-72355390 None:intergenic
TACCGGAGTTCCTAACAGAA+GGG 0.630611 1.2:-72355758 None:intergenic
ACACATTTCTGTACAATCTG+TGG 0.631898 1.2:+72356065 MS.gene60814:CDS
ATCACAGACTGAGTTCGAGA+TGG 0.643055 1.2:-72355483 None:intergenic
AAGCTTGTGTGCTAACAACT+GGG 0.643592 1.2:+72355347 MS.gene60814:CDS
CCTTCTGAATCTTCAAACGG+AGG 0.643624 1.2:-72356728 None:intergenic
AATCTTCGAATGCACATGCG+AGG 0.648293 1.2:+72356110 MS.gene60814:CDS
TCATGAGAAACACTGTGGCA+AGG 0.649401 1.2:+72356385 MS.gene60814:CDS
TGACTGAAAACTTCTTCGTG+TGG 0.651786 1.2:-72356346 None:intergenic
CTGTGGCAAGGGTTTCAAGA+GGG 0.652480 1.2:+72356082 MS.gene60814:CDS
AGAAATGTGTGTGAGGTGCA+AGG 0.661202 1.2:-72356052 None:intergenic
CCGAACCGAAGCTTATCAAG+AGG 0.666381 1.2:+72356192 MS.gene60814:CDS
TCTGTGGCAAGGGTTTCAAG+AGG 0.676093 1.2:+72356081 MS.gene60814:CDS
CATTTGTAACCCATTCCCAG+TGG 0.676566 1.2:-72355391 None:intergenic
ATTCCTTTGGAAGACCACAA+AGG 0.685978 1.2:+72356497 MS.gene60814:CDS
TCTTGAGTCACAAGTTCACG+CGG 0.701065 1.2:-72355666 None:intergenic
ATTGGTGCAAAACAATACTG+TGG 0.704627 1.2:+72355935 MS.gene60814:CDS
ATTGTACAGAAATGTGTGTG+AGG 0.743882 1.2:-72356059 None:intergenic
AAAACTCATGAGAAACACTG+TGG 0.781827 1.2:+72356380 MS.gene60814:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGGTGAGAAATAATTGAT+AGG - chr1.2:72356662-72356681 None:intergenic 25.0%
! GTTACAAATGAATTTGTCTT+CGG + chr1.2:72355404-72355423 MS.gene60814:CDS 25.0%
! TGTACCTAATAATTGTGAAT+TGG + chr1.2:72355917-72355936 MS.gene60814:CDS 25.0%
! TTACAAATGAATTTGTCTTC+GGG + chr1.2:72355405-72355424 MS.gene60814:CDS 25.0%
!! GTTGAATAAGCTTTCATTTT+TGG + chr1.2:72355572-72355591 MS.gene60814:CDS 25.0%
AAATGAGTAAACTTCAAGAC+TGG + chr1.2:72355535-72355554 MS.gene60814:CDS 30.0%
AAGCTTATTCAACATAACAC+TGG - chr1.2:72355565-72355584 None:intergenic 30.0%
AATGAGTAAACTTCAAGACT+GGG + chr1.2:72355536-72355555 MS.gene60814:CDS 30.0%
ACACATAAAATAGTCTTCAG+AGG - chr1.2:72356272-72356291 None:intergenic 30.0%
TCAAATCCTTCAAGTGAAAA+TGG + chr1.2:72356596-72356615 MS.gene60814:CDS 30.0%
TCAGTGATCTTAATATCATC+CGG - chr1.2:72355510-72355529 None:intergenic 30.0%
TGATATTAAGATCACTGATC+AGG + chr1.2:72355512-72355531 MS.gene60814:CDS 30.0%
TGCACCAATTCACAATTATT+AGG - chr1.2:72355924-72355943 None:intergenic 30.0%
TGTAAAATCTCATATGAACC+AGG - chr1.2:72356014-72356033 None:intergenic 30.0%
TTTAATTATGCTCCTAAGTC+TGG + chr1.2:72356779-72356798 MS.gene60814:CDS 30.0%
AAAACTCATGAGAAACACTG+TGG + chr1.2:72356380-72356399 MS.gene60814:CDS 35.0%
AAATGGAGCTGATAGCATTA+TGG + chr1.2:72356613-72356632 MS.gene60814:CDS 35.0%
AATTCCCACACTGTAAAAAG+GGG - chr1.2:72355436-72355455 None:intergenic 35.0%
ACACATTTCTGTACAATCTG+TGG + chr1.2:72356065-72356084 MS.gene60814:CDS 35.0%
ACATAAAGACGAAGAAGATG+TGG + chr1.2:72355968-72355987 MS.gene60814:CDS 35.0%
ATTAGGTACATCCTTCTGAA+TGG - chr1.2:72355907-72355926 None:intergenic 35.0%
ATTGGTGCAAAACAATACTG+TGG + chr1.2:72355935-72355954 MS.gene60814:CDS 35.0%
ATTGTACAGAAATGTGTGTG+AGG - chr1.2:72356062-72356081 None:intergenic 35.0%
CAAAACCACACAAAGAAACT+GGG + chr1.2:72356168-72356187 MS.gene60814:CDS 35.0%
CAATTCCCACACTGTAAAAA+GGG - chr1.2:72355437-72355456 None:intergenic 35.0%
GAATACCTCTTGATAAGCTT+CGG - chr1.2:72356200-72356219 None:intergenic 35.0%
GATAGCATTATGGATGTGAA+AGG + chr1.2:72356623-72356642 MS.gene60814:CDS 35.0%
TCAATTCCCACACTGTAAAA+AGG - chr1.2:72355438-72355457 None:intergenic 35.0%
TTACAGCCTTCAAAGTTCAA+AGG - chr1.2:72356680-72356699 None:intergenic 35.0%
TTCTCACCTTTGAACTTTGA+AGG + chr1.2:72356671-72356690 MS.gene60814:CDS 35.0%
TTTCAATCAGTCCATTCAGA+AGG + chr1.2:72355893-72355912 MS.gene60814:CDS 35.0%
! AACTTTGAAGGCTGTAACTT+TGG + chr1.2:72356683-72356702 MS.gene60814:CDS 35.0%
! CTCTTTCTCTTTTCTCATGT+CGG + chr1.2:72356751-72356770 MS.gene60814:CDS 35.0%
! GGTTGGTTGATTTCTTTGAA+AGG - chr1.2:72355465-72355484 None:intergenic 35.0%
! TCAAGGAAAGACAAGCTTTT+CGG + chr1.2:72356440-72356459 MS.gene60814:CDS 35.0%
! TCTTTCTCTTTTCTCATGTC+GGG + chr1.2:72356752-72356771 MS.gene60814:CDS 35.0%
!! GATAAATGGCTTTGTTCATG+TGG + chr1.2:72356407-72356426 MS.gene60814:CDS 35.0%
!! TTCTTTGTGTGGTTTTGCAA+GGG - chr1.2:72356165-72356184 None:intergenic 35.0%
!! TTTCTTTGTGTGGTTTTGCA+AGG - chr1.2:72356166-72356185 None:intergenic 35.0%
!!! GTAGTTGCACTGTTATTTTG+TGG - chr1.2:72355870-72355889 None:intergenic 35.0%
AACACTGTGGCAAGGATAAA+TGG + chr1.2:72356393-72356412 MS.gene60814:CDS 40.0%
AAGCTTGTGTGCTAACAACT+GGG + chr1.2:72355347-72355366 MS.gene60814:CDS 40.0%
AATTATGCTCCTAAGTCTGG+TGG + chr1.2:72356782-72356801 MS.gene60814:CDS 40.0%
AGAAAAGATTCATCAGCTCC+AGG + chr1.2:72355596-72355615 MS.gene60814:CDS 40.0%
ATTTGTAACCCATTCCCAGT+GGG - chr1.2:72355393-72355412 None:intergenic 40.0%
CAACTTGTGCTTGTTCCAAA+AGG - chr1.2:72355837-72355856 None:intergenic 40.0%
CAATCAAATGCACAAGATCC+TGG - chr1.2:72355617-72355636 None:intergenic 40.0%
GAGCCTTCTGAATCTTCAAA+CGG - chr1.2:72356734-72356753 None:intergenic 40.0%
GCAAAACCACACAAAGAAAC+TGG + chr1.2:72356167-72356186 MS.gene60814:CDS 40.0%
TGAATCTTCAAACGGAGGTT+GGG - chr1.2:72356726-72356745 None:intergenic 40.0%
TGACTGAAAACTTCTTCGTG+TGG - chr1.2:72356349-72356368 None:intergenic 40.0%
TTCAGTTGATCTCTACTGCA+GGG + chr1.2:72355727-72355746 MS.gene60814:CDS 40.0%
TTCTGTACAATCTGTGGCAA+GGG + chr1.2:72356071-72356090 MS.gene60814:CDS 40.0%
TTGTGCTTGTTCCAAAAGGA+AGG - chr1.2:72355833-72355852 None:intergenic 40.0%
TTTCTGTACAATCTGTGGCA+AGG + chr1.2:72356070-72356089 MS.gene60814:CDS 40.0%
TTTGAAGGCTGTAACTTTGG+CGG + chr1.2:72356686-72356705 MS.gene60814:CDS 40.0%
! ATTCCTTTGGAAGACCACAA+AGG + chr1.2:72356497-72356516 MS.gene60814:CDS 40.0%
! CACAGTGTGTTCTTTTGTAG+TGG - chr1.2:72356301-72356320 None:intergenic 40.0%
! CACGAAGAAGTTTTCAGTCA+TGG + chr1.2:72356349-72356368 MS.gene60814:CDS 40.0%
! CATGTGGCACAACATTTTCA+AGG + chr1.2:72356423-72356442 MS.gene60814:CDS 40.0%
! TCAGCTCCATTTTCACTTGA+AGG - chr1.2:72356605-72356624 None:intergenic 40.0%
! TTTGGAACAAGCACAAGTTG+TGG + chr1.2:72355837-72355856 MS.gene60814:CDS 40.0%
!! GAGTTTTGTACTCATCACCA+TGG - chr1.2:72356136-72356155 None:intergenic 40.0%
!! GGAAGCATGAACTTCTGTTT+TGG + chr1.2:72356572-72356591 MS.gene60814:CDS 40.0%
AATCTTCGAATGCACATGCG+AGG + chr1.2:72356110-72356129 MS.gene60814:CDS 45.0%
ACAAACACAAGTCCGTTGCT+TGG + chr1.2:72355780-72355799 MS.gene60814:CDS 45.0%
AGAAATGTGTGTGAGGTGCA+AGG - chr1.2:72356055-72356074 None:intergenic 45.0%
AGACGAAGAAGATGTGGAAG+AGG + chr1.2:72355974-72355993 MS.gene60814:CDS 45.0%
AGTTCACGCGGTTGTTCAAA+AGG - chr1.2:72355657-72355676 None:intergenic 45.0%
ATCACAGACTGAGTTCGAGA+TGG - chr1.2:72355486-72355505 None:intergenic 45.0%
CATGATTCACCACCAGACTT+AGG - chr1.2:72356794-72356813 None:intergenic 45.0%
CATTTGTAACCCATTCCCAG+TGG - chr1.2:72355394-72355413 None:intergenic 45.0%
CCTCCGTTTGAAGATTCAGA+AGG + chr1.2:72356728-72356747 MS.gene60814:CDS 45.0%
CCTCTTGATAAGCTTCGGTT+CGG - chr1.2:72356195-72356214 None:intergenic 45.0%
CCTTCTGAATCTTCAAACGG+AGG - chr1.2:72356731-72356750 None:intergenic 45.0%
CGATGTGACACCAGAGAAAA+CGG + chr1.2:72356539-72356558 MS.gene60814:CDS 45.0%
CTGAATCTTCAAACGGAGGT+TGG - chr1.2:72356727-72356746 None:intergenic 45.0%
GAAGCTTGTGTGCTAACAAC+TGG + chr1.2:72355346-72355365 MS.gene60814:CDS 45.0%
GAAGGCTAATCCCGTTAAGT+TGG - chr1.2:72355815-72355834 None:intergenic 45.0%
GACAGCTTAGCCAACTTAAC+GGG + chr1.2:72355802-72355821 MS.gene60814:CDS 45.0%
GACGAAGAAGATGTGGAAGA+GGG + chr1.2:72355975-72355994 MS.gene60814:CDS 45.0%
GATCCTTTGTGGTCTTCCAA+AGG - chr1.2:72356503-72356522 None:intergenic 45.0%
GGACAGCTTAGCCAACTTAA+CGG + chr1.2:72355801-72355820 MS.gene60814:CDS 45.0%
GTTCAGTTGATCTCTACTGC+AGG + chr1.2:72355726-72355745 MS.gene60814:CDS 45.0%
TACCGGAGTTCCTAACAGAA+GGG - chr1.2:72355761-72355780 None:intergenic 45.0%
TCATGAGAAACACTGTGGCA+AGG + chr1.2:72356385-72356404 MS.gene60814:CDS 45.0%
TCGAACTCAGTCTGTGATTC+CGG + chr1.2:72355488-72355507 MS.gene60814:CDS 45.0%
TCTTGAGTCACAAGTTCACG+CGG - chr1.2:72355669-72355688 None:intergenic 45.0%
TGGTCTTCCAAAGGAATAGC+CGG - chr1.2:72356494-72356513 None:intergenic 45.0%
TTAGCACACAAGCTTCCAGT+TGG - chr1.2:72355342-72355361 None:intergenic 45.0%
TTGAAGGCTGTAACTTTGGC+GGG + chr1.2:72356687-72356706 MS.gene60814:CDS 45.0%
! CAACGGACTTGTGTTTGTAC+CGG - chr1.2:72355778-72355797 None:intergenic 45.0%
! CCTTCCCCTTTTTACAGTGT+GGG + chr1.2:72355429-72355448 MS.gene60814:CDS 45.0%
! GGACACATTGCTCTTTTCCA+AGG + chr1.2:72356461-72356480 MS.gene60814:CDS 45.0%
! TCCTTCCCCTTTTTACAGTG+TGG + chr1.2:72355428-72355447 MS.gene60814:CDS 45.0%
! TCTTTTCCAAGGCCATACAC+CGG + chr1.2:72356472-72356491 MS.gene60814:CDS 45.0%
!! AAGAGGTATTCGTGTCCCTA+TGG + chr1.2:72356209-72356228 MS.gene60814:CDS 45.0%
!!! AACGGGATTAGCCTTCCTTT+TGG + chr1.2:72355819-72355838 MS.gene60814:CDS 45.0%
AGGTATTCGTGTCCCTATGG+TGG + chr1.2:72356212-72356231 MS.gene60814:CDS 50.0%
CAGACTGAGTTCGAGATGGT+TGG - chr1.2:72355482-72355501 None:intergenic 50.0%
CCAAAGGAATAGCCGGTGTA+TGG - chr1.2:72356487-72356506 None:intergenic 50.0%
CCCACACTGTAAAAAGGGGA+AGG - chr1.2:72355432-72355451 None:intergenic 50.0%
CCGAACCGAAGCTTATCAAG+AGG + chr1.2:72356192-72356211 MS.gene60814:CDS 50.0%
CTCCCTTCTGTTAGGAACTC+CGG + chr1.2:72355756-72355775 MS.gene60814:CDS 50.0%
CTGTGGCAAGGGTTTCAAGA+GGG + chr1.2:72356082-72356101 MS.gene60814:CDS 50.0%
GGAGTCTTCTCCCTTCTGTT+AGG + chr1.2:72355748-72355767 MS.gene60814:CDS 50.0%
GTACCGGAGTTCCTAACAGA+AGG - chr1.2:72355762-72355781 None:intergenic 50.0%
TCGGACCCAGTTTCTTTGTG+TGG - chr1.2:72356176-72356195 None:intergenic 50.0%
TCTGTGGCAAGGGTTTCAAG+AGG + chr1.2:72356081-72356100 MS.gene60814:CDS 50.0%
TGGCTAAGCTGTCCAAGCAA+CGG - chr1.2:72355795-72355814 None:intergenic 50.0%
TGTGTGCTAACAACTGGGCT+AGG + chr1.2:72355352-72355371 MS.gene60814:CDS 50.0%
TTACGCTTGCAGCCACCATA+GGG - chr1.2:72356227-72356246 None:intergenic 50.0%
! CCATACACCGGCTATTCCTT+TGG + chr1.2:72356484-72356503 MS.gene60814:CDS 50.0%
! TACCACAGCACCGTTTTCTC+TGG - chr1.2:72356552-72356571 None:intergenic 50.0%
!! AGAGAAAACGGTGCTGTGGT+AGG + chr1.2:72356551-72356570 MS.gene60814:CDS 50.0%
!! TCTCTGGTGTCACATCGATC+AGG - chr1.2:72356536-72356555 None:intergenic 50.0%
GAATAGCCGGTGTATGGCCT+TGG - chr1.2:72356481-72356500 None:intergenic 55.0%
GAGGGTGAGAATCTTGCTCC+TGG + chr1.2:72355993-72356012 MS.gene60814:CDS 55.0%
GTCACATCGATCAGGTGGCT+CGG - chr1.2:72356528-72356547 None:intergenic 55.0%
GTTACGCTTGCAGCCACCAT+AGG - chr1.2:72356228-72356247 None:intergenic 55.0%
TGGTGGCTGCAAGCGTAACA+AGG + chr1.2:72356229-72356248 MS.gene60814:CDS 55.0%
! CACCAGAGAAAACGGTGCTG+TGG + chr1.2:72356547-72356566 MS.gene60814:CDS 55.0%
!! CTGGTGTCACATCGATCAGG+TGG - chr1.2:72356533-72356552 None:intergenic 55.0%
!! TGTGGTTTTGCAAGGGCTGC+TGG - chr1.2:72356158-72356177 None:intergenic 55.0%
CCTCTTCCTTGGCTCCCACT+GGG + chr1.2:72355376-72355395 MS.gene60814:CDS 60.0%
CCTTGGCTCCCACTGGGAAT+GGG + chr1.2:72355382-72355401 MS.gene60814:CDS 60.0%
CGAATGCACATGCGAGGCCA+TGG + chr1.2:72356116-72356135 MS.gene60814:CDS 60.0%
TCCTCTTCCTTGGCTCCCAC+TGG + chr1.2:72355375-72355394 MS.gene60814:CDS 60.0%
TCCTTGGCTCCCACTGGGAA+TGG + chr1.2:72355381-72355400 MS.gene60814:CDS 60.0%
! CTGGGCTAGGTCCTCTTCCT+TGG + chr1.2:72355365-72355384 MS.gene60814:CDS 60.0%
! GTGGCTCGGCAGATCCTTTG+TGG - chr1.2:72356514-72356533 None:intergenic 60.0%
CCCAGTGGGAGCCAAGGAAG+AGG - chr1.2:72355379-72355398 None:intergenic 65.0%
CCCATTCCCAGTGGGAGCCA+AGG - chr1.2:72355385-72355404 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 72355333 72356829 72355333 ID=MS.gene60814
chr1.2 mRNA 72355333 72356829 72355333 ID=MS.gene60814.t1;Parent=MS.gene60814
chr1.2 exon 72355333 72356829 72355333 ID=MS.gene60814.t1.exon1;Parent=MS.gene60814.t1
chr1.2 CDS 72355333 72356829 72355333 ID=cds.MS.gene60814.t1;Parent=MS.gene60814.t1
Gene Sequence

>MS.gene60814

ATGAATCCAACTGGAAGCTTGTGTGCTAACAACTGGGCTAGGTCCTCTTCCTTGGCTCCCACTGGGAATGGGTTACAAATGAATTTGTCTTCGGGTCCTTCCCCTTTTTACAGTGTGGGAATTGAAACACCTTTCAAAGAAATCAACCAACCATCTCGAACTCAGTCTGTGATTCCGGATGATATTAAGATCACTGATCAGGAAATGAGTAAACTTCAAGACTGGGATTCCAGTGTTATGTTGAATAAGCTTTCATTTTTGGAAGAAAAGATTCATCAGCTCCAGGATCTTGTGCATTTGATTGATAGTAAGAAGTGTCAACCTTTTGAACAACCGCGTGAACTTGTGACTCAAGAACAACAACTCATCACAGCTGATTTAACATCAATTATTGTTCAGTTGATCTCTACTGCAGGGAGTCTTCTCCCTTCTGTTAGGAACTCCGGTACAAACACAAGTCCGTTGCTTGGACAGCTTAGCCAACTTAACGGGATTAGCCTTCCTTTTGGAACAAGCACAAGTTGTGGTATTCAGCCACAAAATAACAGTGCAACTACATTTTTCAATCAGTCCATTCAGAAGGATGTACCTAATAATTGTGAATTGGTGCAAAACAATACTGTGGAAGAACATGAACATAAAGACGAAGAAGATGTGGAAGAGGGTGAGAATCTTGCTCCTGGTTCATATGAGATTTTACAACTAGAAAAAGAAGAAATCCTTGCACCTCACACACATTTCTGTACAATCTGTGGCAAGGGTTTCAAGAGGGATGCAAATCTTCGAATGCACATGCGAGGCCATGGTGATGAGTACAAAACTCCAGCAGCCCTTGCAAAACCACACAAAGAAACTGGGTCCGAACCGAAGCTTATCAAGAGGTATTCGTGTCCCTATGGTGGCTGCAAGCGTAACAAGGATCACAAGAAATTTCAGCCTCTGAAGACTATTTTATGTGTCAAAAACCACTACAAAAGAACACACTGTGATAAGAGTTACACTTGCAGCAGATGCCACACGAAGAAGTTTTCAGTCATGGCAGATCTGAAAACTCATGAGAAACACTGTGGCAAGGATAAATGGCTTTGTTCATGTGGCACAACATTTTCAAGGAAAGACAAGCTTTTCGGACACATTGCTCTTTTCCAAGGCCATACACCGGCTATTCCTTTGGAAGACCACAAAGGATCTGCCGAGCCACCTGATCGATGTGACACCAGAGAAAACGGTGCTGTGGTAGGAAGCATGAACTTCTGTTTTGGATCAAATCCTTCAAGTGAAAATGGAGCTGATAGCATTATGGATGTGAAAGGTAATATTGATGATCCTATCAATTATTTCTCACCTTTGAACTTTGAAGGCTGTAACTTTGGCGGGTTTAATGAATTCTCCCAACCTCCGTTTGAAGATTCAGAAGGCTCTTTCTCTTTTCTCATGTCGGGTTCGTTTAATTATGCTCCTAAGTCTGGTGGTGAATCATGTTCTGACAATCTGTAA
Protein sequence

>MS.gene60814.t1

MNPTGSLCANNWARSSSLAPTGNGLQMNLSSGPSPFYSVGIETPFKEINQPSRTQSVIPDDIKITDQEMSKLQDWDSSVMLNKLSFLEEKIHQLQDLVHLIDSKKCQPFEQPRELVTQEQQLITADLTSIIVQLISTAGSLLPSVRNSGTNTSPLLGQLSQLNGISLPFGTSTSCGIQPQNNSATTFFNQSIQKDVPNNCELVQNNTVEEHEHKDEEDVEEGENLAPGSYEILQLEKEEILAPHTHFCTICGKGFKRDANLRMHMRGHGDEYKTPAALAKPHKETGSEPKLIKRYSCPYGGCKRNKDHKKFQPLKTILCVKNHYKRTHCDKSYTCSRCHTKKFSVMADLKTHEKHCGKDKWLCSCGTTFSRKDKLFGHIALFQGHTPAIPLEDHKGSAEPPDRCDTRENGAVVGSMNFCFGSNPSSENGADSIMDVKGNIDDPINYFSPLNFEGCNFGGFNEFSQPPFEDSEGSFSFLMSGSFNYAPKSGGESCSDNL