AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33973

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33973.t1 MTR_1g093095 41.447 152 83 6 239 387 107 255 2.59e-26 108
MS.gene33973.t1 MTR_1g112270 36.076 158 88 5 224 381 63 207 1.57e-24 107
MS.gene33973.t1 MTR_1g112270 35.762 151 85 4 231 381 58 196 2.98e-24 106
MS.gene33973.t1 MTR_4g059870 34.868 152 86 4 231 381 52 191 3.52e-24 105
MS.gene33973.t1 MTR_8g017210 34.731 167 92 5 219 381 61 214 7.94e-24 105
MS.gene33973.t1 MTR_2g099990 34.503 171 95 5 215 381 41 198 3.18e-23 102
MS.gene33973.t1 MTR_2g093960 35.099 151 87 4 231 381 46 185 8.74e-23 100
MS.gene33973.t1 MTR_1g094115 33.775 151 88 4 231 381 61 199 9.51e-23 101
MS.gene33973.t1 MTR_1g016010 35.099 151 87 3 231 381 55 194 3.52e-22 99.4
MS.gene33973.t1 MTR_2g099990 36.170 141 78 3 241 381 1 129 5.55e-21 95.5
MS.gene33973.t1 MTR_2g090745 31.765 170 101 4 215 381 2 159 8.73e-21 95.1
MS.gene33973.t1 MTR_1g094115 35.000 140 79 4 242 381 55 182 1.13e-20 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33973.t1 AT1G34370 54.951 515 194 8 1 496 1 496 1.90e-178 510
MS.gene33973.t1 AT1G34370 54.951 515 194 8 1 496 1 496 1.90e-178 510
MS.gene33973.t1 AT1G34370 70.588 289 75 4 214 496 2 286 3.20e-141 408
MS.gene33973.t1 AT5G22890 40.401 349 178 10 64 401 39 368 9.58e-76 243
MS.gene33973.t1 AT1G51220 37.576 165 89 6 228 387 160 315 1.06e-25 108
MS.gene33973.t1 AT1G34790 40.645 155 79 7 238 387 136 282 1.94e-25 106
MS.gene33973.t1 AT1G13290 38.764 178 96 8 220 392 76 245 2.33e-25 106
MS.gene33973.t1 AT3G20880 39.073 151 82 5 240 387 250 393 1.16e-24 106
MS.gene33973.t1 AT1G08290 40.000 150 81 5 241 387 175 318 1.70e-24 104
MS.gene33973.t1 AT3G45260 33.918 171 98 4 231 401 53 208 2.90e-24 105
MS.gene33973.t1 AT3G45260 33.918 171 98 4 231 401 53 208 2.90e-24 105
MS.gene33973.t1 AT5G44160 34.437 151 87 3 231 381 51 189 3.88e-24 105
MS.gene33973.t1 AT5G66730 34.437 151 87 3 231 381 46 184 5.07e-24 105
MS.gene33973.t1 AT5G44160 34.437 151 87 3 231 381 12 150 5.34e-24 104
MS.gene33973.t1 AT3G57670 36.364 176 96 6 215 382 181 348 7.46e-24 103
MS.gene33973.t1 AT5G60470 35.135 148 85 3 231 378 58 194 1.45e-23 103
MS.gene33973.t1 AT1G03840 34.437 151 87 3 231 381 55 193 1.66e-23 103
MS.gene33973.t1 AT5G60470 35.135 148 85 3 231 378 54 190 1.70e-23 103
MS.gene33973.t1 AT1G03840 34.437 151 87 3 231 381 53 191 1.77e-23 103
MS.gene33973.t1 AT5G60470 35.135 148 85 3 231 378 49 185 1.84e-23 103
MS.gene33973.t1 AT5G03150 35.294 153 84 4 231 381 67 206 2.21e-23 103
MS.gene33973.t1 AT1G14580 33.775 151 88 3 231 381 67 205 3.80e-23 102
MS.gene33973.t1 AT1G14580 33.775 151 88 3 231 381 67 205 3.80e-23 102
MS.gene33973.t1 AT1G14580 33.775 151 88 3 231 381 81 219 4.26e-23 102
MS.gene33973.t1 AT1G55110 33.775 151 89 3 231 381 77 216 7.14e-23 101
MS.gene33973.t1 AT1G55110 33.775 151 89 3 231 381 77 216 7.14e-23 101
MS.gene33973.t1 AT1G55110 33.775 151 89 3 231 381 77 216 7.14e-23 101
MS.gene33973.t1 AT2G02080 33.775 151 88 3 231 381 68 206 8.64e-23 102
MS.gene33973.t1 AT2G02080 33.775 151 88 3 231 381 68 206 8.64e-23 102
MS.gene33973.t1 AT2G02080 33.775 151 88 3 231 381 68 206 8.64e-23 102
MS.gene33973.t1 AT2G02080 33.775 151 88 3 231 381 68 206 8.64e-23 102
MS.gene33973.t1 AT3G50700 35.762 151 85 3 231 381 48 186 1.02e-22 101
MS.gene33973.t1 AT2G02070 33.113 151 89 3 231 381 66 204 1.50e-22 101
MS.gene33973.t1 AT2G02070 33.113 151 89 3 231 381 66 204 1.50e-22 101
MS.gene33973.t1 AT3G13810 34.437 151 88 3 231 381 79 218 1.77e-22 100
MS.gene33973.t1 AT3G13810 34.437 151 88 3 231 381 84 223 1.87e-22 100
MS.gene33973.t1 AT3G13810 34.437 151 88 3 231 381 84 223 1.87e-22 100
MS.gene33973.t1 AT3G13810 35.220 159 91 4 223 381 78 224 1.88e-22 100
MS.gene33973.t1 AT3G13810 35.220 159 91 4 223 381 64 210 1.93e-22 100
MS.gene33973.t1 AT3G13810 34.437 151 88 3 231 381 65 204 2.14e-22 100
MS.gene33973.t1 AT4G02670 35.099 151 87 3 231 381 69 208 1.84e-21 97.1
MS.gene33973.t1 AT4G02670 35.099 151 87 3 231 381 67 206 2.41e-21 96.7
MS.gene33973.t1 AT2G02080 34.752 141 80 3 241 381 1 129 1.17e-20 94.7
MS.gene33973.t1 AT2G02080 34.752 141 80 3 241 381 1 129 1.17e-20 94.7
MS.gene33973.t1 AT1G25250 33.333 159 95 5 221 378 14 162 9.19e-18 85.1
MS.gene33973.t1 AT1G68130 34.899 149 85 6 231 378 55 192 3.04e-17 84.3
MS.gene33973.t1 AT1G25250 34.228 149 87 5 231 378 47 185 4.25e-17 83.6
MS.gene33973.t1 AT2G01940 32.886 149 88 5 231 378 58 195 7.12e-17 83.2
MS.gene33973.t1 AT2G01940 32.847 137 80 5 231 366 58 183 3.23e-14 75.1

Find 107 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATTCCCACCCTGTAAAA+AGG 0.182953 1.4:+74783838 None:intergenic
TCAAATCCTTCAAGTGAAAA+TGG 0.239095 1.4:-74782677 MS.gene33973:CDS
CATGATTCACCACCAGATTT+AGG 0.245532 1.4:+74782482 None:intergenic
TTTAATTATGCTCCTAAATC+TGG 0.266493 1.4:-74782494 MS.gene33973:CDS
TGCACCATTTCACAATTATT+AGG 0.289796 1.4:+74783352 None:intergenic
AACTTTGAAGGCTGTAACTT+TGG 0.308561 1.4:-74782590 MS.gene33973:CDS
AACACTGTGGCAAGGATAAA+TGG 0.316031 1.4:-74782880 MS.gene33973:CDS
GCAAAACCACACAAAGAAAC+TGG 0.316849 1.4:-74783106 MS.gene33973:CDS
TCGAACTCAGTCTGTGATTC+CGG 0.317053 1.4:-74783785 MS.gene33973:CDS
GGAAGCATGAACTTCTGTTT+TGG 0.324815 1.4:-74782701 MS.gene33973:CDS
AACGGGATTAGCCTTCCTTT+TGG 0.342253 1.4:-74783454 MS.gene33973:CDS
TTCTCACCTTTGAACTTTGA+AGG 0.355262 1.4:-74782602 MS.gene33973:CDS
GGAGTCTTCTCCCTTCTGTT+AGG 0.361673 1.4:-74783525 MS.gene33973:CDS
CTGGGCTAGGTCCTCTTCCT+TGG 0.390105 1.4:-74783908 MS.gene33973:CDS
GAAGCTTGTGTGCTAACAAC+TGG 0.402631 1.4:-74783927 MS.gene33973:CDS
AAATGGATCTGATAGCATTA+TGG 0.409807 1.4:-74782660 MS.gene33973:CDS
GACAGCTTAGCCAACTTAAC+GGG 0.414271 1.4:-74783471 MS.gene33973:CDS
GAATAGCCGGTGTATGGCCT+TGG 0.434473 1.4:+74782795 None:intergenic
AAGATTGAAATGAATCCAAC+TGG 0.437188 1.4:-74783949 None:intergenic
AGTTCACGCGGTTGTTCAAA+AGG 0.437372 1.4:+74783619 None:intergenic
ACATAAAGACGAAGAAGATA+TGG 0.453200 1.4:-74783305 MS.gene33973:CDS
GTGGCTCGGCAGATCCTTTG+TGG 0.455339 1.4:+74782762 None:intergenic
GAATCTTCAAATGGAGGTTG+AGG 0.464007 1.4:+74782551 None:intergenic
CAATCAAATGCACAAGATCC+TGG 0.464133 1.4:+74783659 None:intergenic
GAGCCTTCTGAATCTTCAAA+TGG 0.464855 1.4:+74782542 None:intergenic
TGATATTAAGATCACTGATC+AGG 0.471924 1.4:-74783761 MS.gene33973:CDS
GTTACGCTTGCAGCCACCAT+AGG 0.472872 1.4:+74783048 None:intergenic
CCATACACCGGCTATTCCTT+CGG 0.472983 1.4:-74782789 MS.gene33973:CDS
AAACGAGTAAACTTCAAGAC+TGG 0.479681 1.4:-74783738 MS.gene33973:CDS
GGACAGCTTAGCCAACTTAA+CGG 0.484196 1.4:-74783472 MS.gene33973:CDS
CGATGTGACACCAGAGAAAA+CGG 0.484392 1.4:-74782734 MS.gene33973:CDS
GAATACCTCTTGATAAGCTT+CGG 0.485383 1.4:+74783076 None:intergenic
AAAGGTGAGAAATAATTGAT+AGG 0.486126 1.4:+74782614 None:intergenic
CCGAAGGAATAGCCGGTGTA+TGG 0.486962 1.4:+74782789 None:intergenic
TTGAAGGCTGTAACTTTGGT+GGG 0.487577 1.4:-74782586 MS.gene33973:CDS
ATTAGGTACATCCTTCTGAA+TGG 0.490088 1.4:+74783369 None:intergenic
TTCTGTACAATCTGTGGCAA+GGG 0.495568 1.4:-74783202 MS.gene33973:CDS
GTTCAGTTGATCTCTACTGC+AGG 0.497725 1.4:-74783547 MS.gene33973:CDS
CTCCCTTCTGTTAGGAACTC+CGG 0.500730 1.4:-74783517 MS.gene33973:CDS
TTTGAAGGCTGTAACTTTGG+TGG 0.506070 1.4:-74782587 MS.gene33973:CDS
CAACTTGTGCCTGTTCCAAA+AGG 0.509708 1.4:+74783439 None:intergenic
TCTCTGGTGTCACATCGATC+AGG 0.512441 1.4:+74782740 None:intergenic
CCTCTTGATAAGCTTCGGTT+CGG 0.514238 1.4:+74783081 None:intergenic
GAAGGCTAATCCCGTTAAGT+TGG 0.516535 1.4:+74783461 None:intergenic
ACAAACACAAGTCCGTTGCT+TGG 0.519115 1.4:-74783493 MS.gene33973:CDS
GACGAAGAAGATATGGAAGA+GGG 0.519377 1.4:-74783298 MS.gene33973:CDS
TGTACCTAATAATTGTGAAA+TGG 0.520470 1.4:-74783356 MS.gene33973:CDS
AGACGAAGAAGATATGGAAG+AGG 0.521674 1.4:-74783299 MS.gene33973:CDS
CGAATGCACATGCGAGGCCA+TGG 0.526902 1.4:-74783157 MS.gene33973:CDS
AATCTTCAAATGGAGGTTGA+GGG 0.529470 1.4:+74782552 None:intergenic
AAGAGGTATTCGTGTCCCTA+TGG 0.530145 1.4:-74783064 MS.gene33973:CDS
GGTTGGTTGATTTCTTTGAA+AGG 0.533908 1.4:+74783811 None:intergenic
TTTGGAACAGGCACAAGTTG+TGG 0.534584 1.4:-74783436 MS.gene33973:CDS
TGTGTGCTAACAACTGGGCT+AGG 0.534923 1.4:-74783921 MS.gene33973:CDS
GAGGGTGAGAATCTTGCTCC+TGG 0.536278 1.4:-74783280 MS.gene33973:CDS
AGAAAAGATTCATCAGCTCC+AGG 0.538362 1.4:-74783677 MS.gene33973:CDS
GTCACATCGATCAGGTGGCT+CGG 0.538841 1.4:+74782748 None:intergenic
TCGGACCCAGTTTCTTTGTG+TGG 0.543092 1.4:+74783100 None:intergenic
TTTCGATCAGTCCATTCAGA+AGG 0.546357 1.4:-74783380 MS.gene33973:CDS
TCAGTGATCTTAATATCATC+CGG 0.547189 1.4:+74783766 None:intergenic
CAACGGACTTGTGTTTGTAC+CGG 0.548811 1.4:+74783498 None:intergenic
TGGTCTTCCGAAGGAATAGC+CGG 0.552762 1.4:+74782782 None:intergenic
GTTTGTAACCCATTCCCAGT+GGG 0.558849 1.4:+74783883 None:intergenic
TTACGCTTGCAGCCACCATA+GGG 0.561037 1.4:+74783049 None:intergenic
CAGACTGAGTTCGAGATGGT+TGG 0.568725 1.4:+74783794 None:intergenic
TTACAGCCTTCAAAGTTCAA+AGG 0.572217 1.4:+74782596 None:intergenic
GTACCGGAGTTCCTAACAGA+AGG 0.573753 1.4:+74783514 None:intergenic
GATAAATGGCTTTGTTCATG+TGG 0.574687 1.4:-74782866 MS.gene33973:CDS
TTTCTGTACAATCTGTGGCA+AGG 0.576014 1.4:-74783203 MS.gene33973:CDS
GATCCTTTGTGGTCTTCCGA+AGG 0.576114 1.4:+74782773 None:intergenic
AACGAGTAAACTTCAAGACT+GGG 0.577049 1.4:-74783737 MS.gene33973:CDS
TTCAGTTGATCTCTACTGCA+GGG 0.580172 1.4:-74783546 MS.gene33973:CDS
TTGTGCCTGTTCCAAAAGGA+AGG 0.586342 1.4:+74783443 None:intergenic
CCTTCTGAATCTTCAAATGG+AGG 0.588246 1.4:+74782545 None:intergenic
CAAAACCACACAAAGAAACT+GGG 0.588364 1.4:-74783105 MS.gene33973:CDS
TGGTGGCTGCAAGCGTAACA+AGG 0.588657 1.4:-74783044 MS.gene33973:CDS
AATTATGCTCCTAAATCTGG+TGG 0.589400 1.4:-74782491 MS.gene33973:CDS
TTAGCACACAAGCTTCCAGT+TGG 0.591117 1.4:+74783934 None:intergenic
AGAGAAAACGGTGCTGTGGT+AGG 0.594886 1.4:-74782722 MS.gene33973:CDS
AAGCTTATTCAACATAACAC+TGG 0.596534 1.4:+74783711 None:intergenic
GATAGCATTATGGATGTGAA+AGG 0.598382 1.4:-74782650 MS.gene33973:CDS
ACACATAAAATAGTCTTCAG+AGG 0.602166 1.4:+74783004 None:intergenic
TTGTAACCCATTCCCAGTGG+GGG 0.606052 1.4:+74783885 None:intergenic
CCTCCATTTGAAGATTCAGA+AGG 0.608580 1.4:-74782545 MS.gene33973:CDS
AGGTATTCGTGTCCCTATGG+TGG 0.612960 1.4:-74783061 MS.gene33973:CDS
TGGCTAAGCTGTCCAAGCAA+CGG 0.613429 1.4:+74783481 None:intergenic
TGTAAAATCTCATATGAACC+AGG 0.616349 1.4:+74783262 None:intergenic
CACCAGAGAAAACGGTGCTG+TGG 0.617127 1.4:-74782726 MS.gene33973:CDS
CTGGTGTCACATCGATCAGG+TGG 0.620999 1.4:+74782743 None:intergenic
TACCGGAGTTCCTAACAGAA+GGG 0.630611 1.4:+74783515 None:intergenic
ACACATTTCTGTACAATCTG+TGG 0.631898 1.4:-74783208 MS.gene33973:CDS
TTTGTAACCCATTCCCAGTG+GGG 0.637498 1.4:+74783884 None:intergenic
ATCACAGACTGAGTTCGAGA+TGG 0.643055 1.4:+74783790 None:intergenic
AAGCTTGTGTGCTAACAACT+GGG 0.643592 1.4:-74783926 MS.gene33973:CDS
CGTTTGTAACCCATTCCCAG+TGG 0.646958 1.4:+74783882 None:intergenic
AATCTTCGAATGCACATGCG+AGG 0.648293 1.4:-74783163 MS.gene33973:CDS
TCATGAGAAACACTGTGGCA+AGG 0.649401 1.4:-74782888 MS.gene33973:CDS
TGACTGAAAACTTCTTCGTG+TGG 0.651786 1.4:+74782927 None:intergenic
CTGTGGCAAGGGTTTCAAGA+GGG 0.652480 1.4:-74783191 MS.gene33973:CDS
AGAAATGTGTGTGAGGTGCA+AGG 0.661202 1.4:+74783221 None:intergenic
CCGAACCGAAGCTTATCAAG+AGG 0.666381 1.4:-74783081 MS.gene33973:CDS
TCTGTGGCAAGGGTTTCAAG+AGG 0.676093 1.4:-74783192 MS.gene33973:CDS
TCTTGAGTCACAAGTTCACG+CGG 0.701065 1.4:+74783607 None:intergenic
AATGGTGCAAAACAATACTG+TGG 0.704627 1.4:-74783338 MS.gene33973:CDS
ATTCCTTCGGAAGACCACAA+AGG 0.713111 1.4:-74782776 MS.gene33973:CDS
ATTGTACAGAAATGTGTGTG+AGG 0.743882 1.4:+74783214 None:intergenic
AAAACTCATGAGAAACACTG+TGG 0.781827 1.4:-74782893 MS.gene33973:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGGTGAGAAATAATTGAT+AGG + chr1.4:74783795-74783814 None:intergenic 25.0%
! TGTACCTAATAATTGTGAAA+TGG - chr1.4:74783050-74783069 MS.gene33973:CDS 25.0%
! TTTAATTATGCTCCTAAATC+TGG - chr1.4:74783912-74783931 MS.gene33973:CDS 25.0%
!! GTTGAATAAGCTTTCATTTT+TGG - chr1.4:74782705-74782724 MS.gene33973:CDS 25.0%
AAATGGATCTGATAGCATTA+TGG - chr1.4:74783746-74783765 MS.gene33973:CDS 30.0%
AAGCTTATTCAACATAACAC+TGG + chr1.4:74782698-74782717 None:intergenic 30.0%
ACACATAAAATAGTCTTCAG+AGG + chr1.4:74783405-74783424 None:intergenic 30.0%
ACATAAAGACGAAGAAGATA+TGG - chr1.4:74783101-74783120 MS.gene33973:CDS 30.0%
TCAAATCCTTCAAGTGAAAA+TGG - chr1.4:74783729-74783748 MS.gene33973:CDS 30.0%
TCAGTGATCTTAATATCATC+CGG + chr1.4:74782643-74782662 None:intergenic 30.0%
TGATATTAAGATCACTGATC+AGG - chr1.4:74782645-74782664 MS.gene33973:CDS 30.0%
TGCACCATTTCACAATTATT+AGG + chr1.4:74783057-74783076 None:intergenic 30.0%
TGTAAAATCTCATATGAACC+AGG + chr1.4:74783147-74783166 None:intergenic 30.0%
AAAACTCATGAGAAACACTG+TGG - chr1.4:74783513-74783532 MS.gene33973:CDS 35.0%
AAACGAGTAAACTTCAAGAC+TGG - chr1.4:74782668-74782687 MS.gene33973:CDS 35.0%
AACGAGTAAACTTCAAGACT+GGG - chr1.4:74782669-74782688 MS.gene33973:CDS 35.0%
AATCTTCAAATGGAGGTTGA+GGG + chr1.4:74783857-74783876 None:intergenic 35.0%
AATGGTGCAAAACAATACTG+TGG - chr1.4:74783068-74783087 MS.gene33973:CDS 35.0%
AATTATGCTCCTAAATCTGG+TGG - chr1.4:74783915-74783934 MS.gene33973:CDS 35.0%
ACACATTTCTGTACAATCTG+TGG - chr1.4:74783198-74783217 MS.gene33973:CDS 35.0%
ATTAGGTACATCCTTCTGAA+TGG + chr1.4:74783040-74783059 None:intergenic 35.0%
ATTGTACAGAAATGTGTGTG+AGG + chr1.4:74783195-74783214 None:intergenic 35.0%
CAAAACCACACAAAGAAACT+GGG - chr1.4:74783301-74783320 MS.gene33973:CDS 35.0%
GAATACCTCTTGATAAGCTT+CGG + chr1.4:74783333-74783352 None:intergenic 35.0%
GATAGCATTATGGATGTGAA+AGG - chr1.4:74783756-74783775 MS.gene33973:CDS 35.0%
TTACAGCCTTCAAAGTTCAA+AGG + chr1.4:74783813-74783832 None:intergenic 35.0%
TTCTCACCTTTGAACTTTGA+AGG - chr1.4:74783804-74783823 MS.gene33973:CDS 35.0%
! AACTTTGAAGGCTGTAACTT+TGG - chr1.4:74783816-74783835 MS.gene33973:CDS 35.0%
! CTCTTTCTCTTTTCTCATGT+CGG - chr1.4:74783884-74783903 MS.gene33973:CDS 35.0%
! GGTTGGTTGATTTCTTTGAA+AGG + chr1.4:74782598-74782617 None:intergenic 35.0%
! TCAAGGAAAGACAAGCTTTT+CGG - chr1.4:74783573-74783592 MS.gene33973:CDS 35.0%
! TCAGATCCATTTTCACTTGA+AGG + chr1.4:74783738-74783757 None:intergenic 35.0%
! TCTTTCTCTTTTCTCATGTC+GGG - chr1.4:74783885-74783904 MS.gene33973:CDS 35.0%
!! GATAAATGGCTTTGTTCATG+TGG - chr1.4:74783540-74783559 MS.gene33973:CDS 35.0%
!! TTCTTTGTGTGGTTTTGCAA+GGG + chr1.4:74783298-74783317 None:intergenic 35.0%
!! TTTCTTTGTGTGGTTTTGCA+AGG + chr1.4:74783299-74783318 None:intergenic 35.0%
!!! GTAGTTGCACTGTTATTTTG+TGG + chr1.4:74783003-74783022 None:intergenic 35.0%
AACACTGTGGCAAGGATAAA+TGG - chr1.4:74783526-74783545 MS.gene33973:CDS 40.0%
AAGCTTGTGTGCTAACAACT+GGG - chr1.4:74782480-74782499 MS.gene33973:CDS 40.0%
AATTCCCACCCTGTAAAAAG+GGG + chr1.4:74782569-74782588 None:intergenic 40.0%
AGAAAAGATTCATCAGCTCC+AGG - chr1.4:74782729-74782748 MS.gene33973:CDS 40.0%
AGACGAAGAAGATATGGAAG+AGG - chr1.4:74783107-74783126 MS.gene33973:CDS 40.0%
CAATCAAATGCACAAGATCC+TGG + chr1.4:74782750-74782769 None:intergenic 40.0%
CAATTCCCACCCTGTAAAAA+GGG + chr1.4:74782570-74782589 None:intergenic 40.0%
CATGATTCACCACCAGATTT+AGG + chr1.4:74783927-74783946 None:intergenic 40.0%
CCTCCATTTGAAGATTCAGA+AGG - chr1.4:74783861-74783880 MS.gene33973:CDS 40.0%
CCTTCTGAATCTTCAAATGG+AGG + chr1.4:74783864-74783883 None:intergenic 40.0%
GAATCTTCAAATGGAGGTTG+AGG + chr1.4:74783858-74783877 None:intergenic 40.0%
GACGAAGAAGATATGGAAGA+GGG - chr1.4:74783108-74783127 MS.gene33973:CDS 40.0%
GAGCCTTCTGAATCTTCAAA+TGG + chr1.4:74783867-74783886 None:intergenic 40.0%
GCAAAACCACACAAAGAAAC+TGG - chr1.4:74783300-74783319 MS.gene33973:CDS 40.0%
TCAATTCCCACCCTGTAAAA+AGG + chr1.4:74782571-74782590 None:intergenic 40.0%
TGACTGAAAACTTCTTCGTG+TGG + chr1.4:74783482-74783501 None:intergenic 40.0%
TTCAGTTGATCTCTACTGCA+GGG - chr1.4:74782860-74782879 MS.gene33973:CDS 40.0%
TTCTGTACAATCTGTGGCAA+GGG - chr1.4:74783204-74783223 MS.gene33973:CDS 40.0%
TTGAAGGCTGTAACTTTGGT+GGG - chr1.4:74783820-74783839 MS.gene33973:CDS 40.0%
TTTCGATCAGTCCATTCAGA+AGG - chr1.4:74783026-74783045 MS.gene33973:CDS 40.0%
TTTCTGTACAATCTGTGGCA+AGG - chr1.4:74783203-74783222 MS.gene33973:CDS 40.0%
TTTGAAGGCTGTAACTTTGG+TGG - chr1.4:74783819-74783838 MS.gene33973:CDS 40.0%
! CACAGTGTGTTCTTTTGTAG+TGG + chr1.4:74783434-74783453 None:intergenic 40.0%
! CACGAAGAAGTTTTCAGTCA+TGG - chr1.4:74783482-74783501 MS.gene33973:CDS 40.0%
! CATGTGGCACAACATTTTCA+AGG - chr1.4:74783556-74783575 MS.gene33973:CDS 40.0%
!! GAGTTTTGTACTCATCACCA+TGG + chr1.4:74783269-74783288 None:intergenic 40.0%
!! GGAAGCATGAACTTCTGTTT+TGG - chr1.4:74783705-74783724 MS.gene33973:CDS 40.0%
!!! ATTAGCCTTCCTTTTGGAAC+AGG - chr1.4:74782958-74782977 MS.gene33973:CDS 40.0%
AATCTTCGAATGCACATGCG+AGG - chr1.4:74783243-74783262 MS.gene33973:CDS 45.0%
ACAAACACAAGTCCGTTGCT+TGG - chr1.4:74782913-74782932 MS.gene33973:CDS 45.0%
AGAAATGTGTGTGAGGTGCA+AGG + chr1.4:74783188-74783207 None:intergenic 45.0%
AGTTCACGCGGTTGTTCAAA+AGG + chr1.4:74782790-74782809 None:intergenic 45.0%
ATCACAGACTGAGTTCGAGA+TGG + chr1.4:74782619-74782638 None:intergenic 45.0%
ATTCCTTCGGAAGACCACAA+AGG - chr1.4:74783630-74783649 MS.gene33973:CDS 45.0%
CAACTTGTGCCTGTTCCAAA+AGG + chr1.4:74782970-74782989 None:intergenic 45.0%
CCTCTTGATAAGCTTCGGTT+CGG + chr1.4:74783328-74783347 None:intergenic 45.0%
CGATGTGACACCAGAGAAAA+CGG - chr1.4:74783672-74783691 MS.gene33973:CDS 45.0%
GAAGCTTGTGTGCTAACAAC+TGG - chr1.4:74782479-74782498 MS.gene33973:CDS 45.0%
GAAGGCTAATCCCGTTAAGT+TGG + chr1.4:74782948-74782967 None:intergenic 45.0%
GACAGCTTAGCCAACTTAAC+GGG - chr1.4:74782935-74782954 MS.gene33973:CDS 45.0%
GGACAGCTTAGCCAACTTAA+CGG - chr1.4:74782934-74782953 MS.gene33973:CDS 45.0%
GTTCAGTTGATCTCTACTGC+AGG - chr1.4:74782859-74782878 MS.gene33973:CDS 45.0%
GTTTGTAACCCATTCCCAGT+GGG + chr1.4:74782526-74782545 None:intergenic 45.0%
TACCGGAGTTCCTAACAGAA+GGG + chr1.4:74782894-74782913 None:intergenic 45.0%
TCATGAGAAACACTGTGGCA+AGG - chr1.4:74783518-74783537 MS.gene33973:CDS 45.0%
TCGAACTCAGTCTGTGATTC+CGG - chr1.4:74782621-74782640 MS.gene33973:CDS 45.0%
TCTTGAGTCACAAGTTCACG+CGG + chr1.4:74782802-74782821 None:intergenic 45.0%
TTAGCACACAAGCTTCCAGT+TGG + chr1.4:74782475-74782494 None:intergenic 45.0%
TTGTGCCTGTTCCAAAAGGA+AGG + chr1.4:74782966-74782985 None:intergenic 45.0%
TTTGTAACCCATTCCCAGTG+GGG + chr1.4:74782525-74782544 None:intergenic 45.0%
! CAACGGACTTGTGTTTGTAC+CGG + chr1.4:74782911-74782930 None:intergenic 45.0%
! GGACACATTGCTCTTTTCCA+AGG - chr1.4:74783594-74783613 MS.gene33973:CDS 45.0%
! GTGTCCTTCCCCTTTTTACA+GGG - chr1.4:74782558-74782577 MS.gene33973:CDS 45.0%
! TCTTTTCCAAGGCCATACAC+CGG - chr1.4:74783605-74783624 MS.gene33973:CDS 45.0%
! TTTGGAACAGGCACAAGTTG+TGG - chr1.4:74782970-74782989 MS.gene33973:CDS 45.0%
!! AAGAGGTATTCGTGTCCCTA+TGG - chr1.4:74783342-74783361 MS.gene33973:CDS 45.0%
!!! AACGGGATTAGCCTTCCTTT+TGG - chr1.4:74782952-74782971 MS.gene33973:CDS 45.0%
AGGTATTCGTGTCCCTATGG+TGG - chr1.4:74783345-74783364 MS.gene33973:CDS 50.0%
CAGACTGAGTTCGAGATGGT+TGG + chr1.4:74782615-74782634 None:intergenic 50.0%
CCGAACCGAAGCTTATCAAG+AGG - chr1.4:74783325-74783344 MS.gene33973:CDS 50.0%
CGTTTGTAACCCATTCCCAG+TGG + chr1.4:74782527-74782546 None:intergenic 50.0%
CTCCCTTCTGTTAGGAACTC+CGG - chr1.4:74782889-74782908 MS.gene33973:CDS 50.0%
CTGTGGCAAGGGTTTCAAGA+GGG - chr1.4:74783215-74783234 MS.gene33973:CDS 50.0%
GATCCTTTGTGGTCTTCCGA+AGG + chr1.4:74783636-74783655 None:intergenic 50.0%
GGAGTCTTCTCCCTTCTGTT+AGG - chr1.4:74782881-74782900 MS.gene33973:CDS 50.0%
GTAAAAAGGGGAAGGACACG+AGG + chr1.4:74782557-74782576 None:intergenic 50.0%
GTACCGGAGTTCCTAACAGA+AGG + chr1.4:74782895-74782914 None:intergenic 50.0%
TCGGACCCAGTTTCTTTGTG+TGG + chr1.4:74783309-74783328 None:intergenic 50.0%
TCTGTGGCAAGGGTTTCAAG+AGG - chr1.4:74783214-74783233 MS.gene33973:CDS 50.0%
TGGCTAAGCTGTCCAAGCAA+CGG + chr1.4:74782928-74782947 None:intergenic 50.0%
TGGTCTTCCGAAGGAATAGC+CGG + chr1.4:74783627-74783646 None:intergenic 50.0%
TGTGTGCTAACAACTGGGCT+AGG - chr1.4:74782485-74782504 MS.gene33973:CDS 50.0%
TTACGCTTGCAGCCACCATA+GGG + chr1.4:74783360-74783379 None:intergenic 50.0%
TTGTAACCCATTCCCAGTGG+GGG + chr1.4:74782524-74782543 None:intergenic 50.0%
! CCATACACCGGCTATTCCTT+CGG - chr1.4:74783617-74783636 MS.gene33973:CDS 50.0%
! CCTTCCCCTTTTTACAGGGT+GGG - chr1.4:74782562-74782581 MS.gene33973:CDS 50.0%
! CGTGTCCTTCCCCTTTTTAC+AGG - chr1.4:74782557-74782576 MS.gene33973:CDS 50.0%
! TACCACAGCACCGTTTTCTC+TGG + chr1.4:74783685-74783704 None:intergenic 50.0%
! TCCTTCCCCTTTTTACAGGG+TGG - chr1.4:74782561-74782580 MS.gene33973:CDS 50.0%
!! AGAGAAAACGGTGCTGTGGT+AGG - chr1.4:74783684-74783703 MS.gene33973:CDS 50.0%
!! TCTCTGGTGTCACATCGATC+AGG + chr1.4:74783669-74783688 None:intergenic 50.0%
CCCACCCTGTAAAAAGGGGA+AGG + chr1.4:74782565-74782584 None:intergenic 55.0%
CCGAAGGAATAGCCGGTGTA+TGG + chr1.4:74783620-74783639 None:intergenic 55.0%
GAATAGCCGGTGTATGGCCT+TGG + chr1.4:74783614-74783633 None:intergenic 55.0%
GAGGGTGAGAATCTTGCTCC+TGG - chr1.4:74783126-74783145 MS.gene33973:CDS 55.0%
GTCACATCGATCAGGTGGCT+CGG + chr1.4:74783661-74783680 None:intergenic 55.0%
GTTACGCTTGCAGCCACCAT+AGG + chr1.4:74783361-74783380 None:intergenic 55.0%
TGGTGGCTGCAAGCGTAACA+AGG - chr1.4:74783362-74783381 MS.gene33973:CDS 55.0%
! CACCAGAGAAAACGGTGCTG+TGG - chr1.4:74783680-74783699 MS.gene33973:CDS 55.0%
!! CTGGTGTCACATCGATCAGG+TGG + chr1.4:74783666-74783685 None:intergenic 55.0%
!! TGTGGTTTTGCAAGGGCTGC+TGG + chr1.4:74783291-74783310 None:intergenic 55.0%
CGAATGCACATGCGAGGCCA+TGG - chr1.4:74783249-74783268 MS.gene33973:CDS 60.0%
! CTGGGCTAGGTCCTCTTCCT+TGG - chr1.4:74782498-74782517 MS.gene33973:CDS 60.0%
! GTGGCTCGGCAGATCCTTTG+TGG + chr1.4:74783647-74783666 None:intergenic 60.0%
CCTCTTCCTTGGCCCCCACT+GGG - chr1.4:74782509-74782528 MS.gene33973:CDS 65.0%
CCTTGGCCCCCACTGGGAAT+GGG - chr1.4:74782515-74782534 MS.gene33973:CDS 65.0%
TCCTCTTCCTTGGCCCCCAC+TGG - chr1.4:74782508-74782527 MS.gene33973:CDS 65.0%
TCCTTGGCCCCCACTGGGAA+TGG - chr1.4:74782514-74782533 MS.gene33973:CDS 65.0%
CCCAGTGGGGGCCAAGGAAG+AGG + chr1.4:74782512-74782531 None:intergenic 70.0%
CCCATTCCCAGTGGGGGCCA+AGG + chr1.4:74782518-74782537 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 74782466 74783962 74782466 ID=MS.gene33973
chr1.4 mRNA 74782466 74783962 74782466 ID=MS.gene33973.t1;Parent=MS.gene33973
chr1.4 exon 74782466 74783962 74782466 ID=MS.gene33973.t1.exon1;Parent=MS.gene33973.t1
chr1.4 CDS 74782466 74783962 74782466 ID=cds.MS.gene33973.t1;Parent=MS.gene33973.t1
Gene Sequence

>MS.gene33973

ATGAATCCAACTGGAAGCTTGTGTGCTAACAACTGGGCTAGGTCCTCTTCCTTGGCCCCCACTGGGAATGGGTTACAAACGAATTTGTCCTCGTGTCCTTCCCCTTTTTACAGGGTGGGAATTGAAACACCTTTCAAAGAAATCAACCAACCATCTCGAACTCAGTCTGTGATTCCGGATGATATTAAGATCACTGATCAGGAAACGAGTAAACTTCAAGACTGGGATTCCAGTGTTATGTTGAATAAGCTTTCATTTTTGGAAGAAAAGATTCATCAGCTCCAGGATCTTGTGCATTTGATTGATACTAAGAAGTGTCAACCTTTTGAACAACCGCGTGAACTTGTGACTCAAGAACAACAACTCATCACAGCTGATTTAACATCAATTATTGTTCAGTTGATCTCTACTGCAGGGAGTCTTCTCCCTTCTGTTAGGAACTCCGGTACAAACACAAGTCCGTTGCTTGGACAGCTTAGCCAACTTAACGGGATTAGCCTTCCTTTTGGAACAGGCACAAGTTGTGGTATTCAGCCACAAAATAACAGTGCAACTACATTTTTCGATCAGTCCATTCAGAAGGATGTACCTAATAATTGTGAAATGGTGCAAAACAATACTGTGGAAGAACATGAACATAAAGACGAAGAAGATATGGAAGAGGGTGAGAATCTTGCTCCTGGTTCATATGAGATTTTACAACTAGAAAAAGAAGAAATCCTTGCACCTCACACACATTTCTGTACAATCTGTGGCAAGGGTTTCAAGAGGGATGCAAATCTTCGAATGCACATGCGAGGCCATGGTGATGAGTACAAAACTCCAGCAGCCCTTGCAAAACCACACAAAGAAACTGGGTCCGAACCGAAGCTTATCAAGAGGTATTCGTGTCCCTATGGTGGCTGCAAGCGTAACAAGGATCACAAGAAATTTCAGCCTCTGAAGACTATTTTATGTGTCAAAAACCACTACAAAAGAACACACTGTGATAAGAGTTACACTTGCAGCAGATGCCACACGAAGAAGTTTTCAGTCATGGCAGATCTGAAAACTCATGAGAAACACTGTGGCAAGGATAAATGGCTTTGTTCATGTGGCACAACATTTTCAAGGAAAGACAAGCTTTTCGGACACATTGCTCTTTTCCAAGGCCATACACCGGCTATTCCTTCGGAAGACCACAAAGGATCTGCCGAGCCACCTGATCGATGTGACACCAGAGAAAACGGTGCTGTGGTAGGAAGCATGAACTTCTGTTTTGGATCAAATCCTTCAAGTGAAAATGGATCTGATAGCATTATGGATGTGAAAGGTAATATTGATGATCCTATCAATTATTTCTCACCTTTGAACTTTGAAGGCTGTAACTTTGGTGGGTTTAATGAATTCCCTCAACCTCCATTTGAAGATTCAGAAGGCTCTTTCTCTTTTCTCATGTCGGGTTCGTTTAATTATGCTCCTAAATCTGGTGGTGAATCATGTTCTGACAATCTGTAA
Protein sequence

>MS.gene33973.t1

MNPTGSLCANNWARSSSLAPTGNGLQTNLSSCPSPFYRVGIETPFKEINQPSRTQSVIPDDIKITDQETSKLQDWDSSVMLNKLSFLEEKIHQLQDLVHLIDTKKCQPFEQPRELVTQEQQLITADLTSIIVQLISTAGSLLPSVRNSGTNTSPLLGQLSQLNGISLPFGTGTSCGIQPQNNSATTFFDQSIQKDVPNNCEMVQNNTVEEHEHKDEEDMEEGENLAPGSYEILQLEKEEILAPHTHFCTICGKGFKRDANLRMHMRGHGDEYKTPAALAKPHKETGSEPKLIKRYSCPYGGCKRNKDHKKFQPLKTILCVKNHYKRTHCDKSYTCSRCHTKKFSVMADLKTHEKHCGKDKWLCSCGTTFSRKDKLFGHIALFQGHTPAIPSEDHKGSAEPPDRCDTRENGAVVGSMNFCFGSNPSSENGSDSIMDVKGNIDDPINYFSPLNFEGCNFGGFNEFPQPPFEDSEGSFSFLMSGSFNYAPKSGGESCSDNL