Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041610.t1 | XP_013456093.1 | 98.3 | 180 | 3 | 0 | 1 | 180 | 1 | 180 | 4.60E-101 | 377.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041610.t1 | Q9FHE1 | 39.8 | 118 | 69 | 2 | 56 | 172 | 277 | 393 | 2.2e-19 | 97.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041610.t1 | A0A072UK42 | 98.3 | 180 | 3 | 0 | 1 | 180 | 1 | 180 | 3.3e-101 | 377.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene041610 | MS.gene066439 | PPI |
| MS.gene041610 | MS.gene90825 | PPI |
| MS.gene041610 | MS.gene029093 | PPI |
| MS.gene041610 | MS.gene97346 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041610.t1 | MTR_4g063670 | 98.333 | 180 | 3 | 0 | 1 | 180 | 1 | 180 | 3.57e-135 | 380 |
| MS.gene041610.t1 | MTR_3g069460 | 85.057 | 174 | 26 | 0 | 1 | 174 | 1 | 174 | 2.84e-110 | 312 |
| MS.gene041610.t1 | MTR_1g051185 | 81.111 | 180 | 9 | 1 | 1 | 180 | 1 | 155 | 1.56e-102 | 296 |
| MS.gene041610.t1 | MTR_0867s0010 | 66.667 | 180 | 60 | 0 | 1 | 180 | 1 | 180 | 6.49e-86 | 255 |
| MS.gene041610.t1 | MTR_4g083510 | 60.773 | 181 | 70 | 1 | 1 | 180 | 1 | 181 | 7.22e-76 | 230 |
| MS.gene041610.t1 | MTR_7g088160 | 45.570 | 158 | 82 | 3 | 21 | 178 | 76 | 229 | 6.37e-39 | 137 |
| MS.gene041610.t1 | MTR_8g015740 | 63.415 | 82 | 30 | 0 | 1 | 82 | 49 | 130 | 1.25e-32 | 114 |
| MS.gene041610.t1 | MTR_7g107340 | 46.789 | 109 | 56 | 2 | 72 | 180 | 101 | 207 | 1.32e-24 | 98.2 |
| MS.gene041610.t1 | MTR_2g023560 | 65.079 | 63 | 22 | 0 | 45 | 107 | 56 | 118 | 4.35e-22 | 86.7 |
| MS.gene041610.t1 | MTR_6g081150 | 34.839 | 155 | 50 | 4 | 19 | 173 | 16 | 119 | 1.47e-19 | 81.6 |
| MS.gene041610.t1 | MTR_7g114170 | 35.583 | 163 | 69 | 6 | 18 | 180 | 61 | 187 | 1.02e-16 | 76.3 |
| MS.gene041610.t1 | MTR_4g013220 | 28.931 | 159 | 105 | 4 | 22 | 174 | 55 | 211 | 1.51e-16 | 76.3 |
| MS.gene041610.t1 | MTR_6g453050 | 26.744 | 172 | 115 | 4 | 9 | 174 | 45 | 211 | 3.10e-16 | 75.1 |
| MS.gene041610.t1 | MTR_4g026120 | 28.302 | 159 | 106 | 4 | 22 | 174 | 55 | 211 | 8.38e-16 | 73.9 |
| MS.gene041610.t1 | MTR_4g036435 | 28.302 | 159 | 106 | 4 | 22 | 174 | 55 | 211 | 8.38e-16 | 73.9 |
| MS.gene041610.t1 | MTR_3g067785 | 28.302 | 159 | 106 | 4 | 22 | 174 | 55 | 211 | 9.35e-16 | 73.9 |
| MS.gene041610.t1 | MTR_8g015750 | 55.556 | 63 | 27 | 1 | 118 | 180 | 1 | 62 | 1.05e-15 | 71.6 |
| MS.gene041610.t1 | MTR_5g066280 | 28.302 | 159 | 106 | 4 | 22 | 174 | 55 | 211 | 1.10e-15 | 73.6 |
| MS.gene041610.t1 | MTR_2g072860 | 27.326 | 172 | 118 | 5 | 7 | 174 | 17 | 185 | 1.38e-15 | 73.2 |
| MS.gene041610.t1 | MTR_7g461350 | 27.907 | 172 | 117 | 5 | 7 | 174 | 85 | 253 | 1.38e-15 | 73.6 |
| MS.gene041610.t1 | MTR_6g016670 | 27.907 | 172 | 117 | 5 | 7 | 174 | 85 | 253 | 1.38e-15 | 73.6 |
| MS.gene041610.t1 | MTR_3g464310 | 27.907 | 172 | 117 | 5 | 7 | 174 | 85 | 253 | 1.38e-15 | 73.6 |
| MS.gene041610.t1 | MTR_2g022120 | 28.302 | 159 | 106 | 4 | 22 | 174 | 119 | 275 | 1.91e-15 | 73.6 |
| MS.gene041610.t1 | MTR_5g077245 | 29.655 | 145 | 94 | 4 | 36 | 174 | 122 | 264 | 1.94e-15 | 73.2 |
| MS.gene041610.t1 | MTR_1g106885 | 28.302 | 159 | 106 | 4 | 22 | 174 | 116 | 272 | 2.15e-15 | 73.2 |
| MS.gene041610.t1 | MTR_6g092533 | 28.302 | 159 | 106 | 4 | 22 | 174 | 119 | 275 | 2.17e-15 | 73.2 |
| MS.gene041610.t1 | MTR_3g450670 | 25.926 | 162 | 110 | 4 | 19 | 174 | 92 | 249 | 2.79e-15 | 72.8 |
| MS.gene041610.t1 | MTR_1g471070 | 25.926 | 162 | 110 | 4 | 19 | 174 | 92 | 249 | 2.79e-15 | 72.8 |
| MS.gene041610.t1 | MTR_3g464170 | 26.437 | 174 | 117 | 5 | 7 | 174 | 85 | 253 | 2.86e-15 | 72.8 |
| MS.gene041610.t1 | MTR_1g100375 | 26.437 | 174 | 117 | 5 | 7 | 174 | 85 | 253 | 2.86e-15 | 72.8 |
| MS.gene041610.t1 | MTR_7g062380 | 27.027 | 148 | 100 | 4 | 33 | 174 | 1 | 146 | 9.10e-15 | 70.5 |
| MS.gene041610.t1 | MTR_6g051405 | 28.448 | 116 | 81 | 2 | 59 | 174 | 140 | 253 | 9.89e-15 | 71.2 |
| MS.gene041610.t1 | MTR_7g083520 | 26.786 | 168 | 112 | 4 | 9 | 170 | 19 | 181 | 1.13e-14 | 70.5 |
| MS.gene041610.t1 | MTR_1g086750 | 30.714 | 140 | 89 | 4 | 38 | 171 | 6 | 143 | 2.70e-14 | 68.6 |
| MS.gene041610.t1 | MTR_5g044920 | 77.778 | 36 | 8 | 0 | 47 | 82 | 21 | 56 | 1.08e-12 | 61.6 |
| MS.gene041610.t1 | MTR_4g046090 | 29.126 | 103 | 71 | 2 | 72 | 174 | 71 | 171 | 1.32e-11 | 61.6 |
| MS.gene041610.t1 | MTR_6g069240 | 28.846 | 104 | 72 | 2 | 71 | 174 | 19 | 120 | 3.40e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041610.t1 | AT3G47680 | 38.255 | 149 | 84 | 4 | 30 | 172 | 23 | 169 | 2.57e-26 | 102 |
| MS.gene041610.t1 | AT5G41220 | 39.831 | 118 | 69 | 2 | 56 | 172 | 219 | 335 | 4.51e-22 | 92.8 |
| MS.gene041610.t1 | AT5G41220 | 39.831 | 118 | 69 | 2 | 56 | 172 | 227 | 343 | 4.61e-22 | 92.8 |
| MS.gene041610.t1 | AT5G41220 | 39.831 | 118 | 69 | 2 | 56 | 172 | 277 | 393 | 4.93e-22 | 92.8 |
| MS.gene041610.t1 | AT5G41240 | 36.154 | 130 | 79 | 3 | 46 | 172 | 267 | 395 | 4.01e-20 | 87.0 |
| MS.gene041610.t1 | AT1G66235 | 28.682 | 129 | 89 | 2 | 52 | 178 | 7 | 134 | 3.58e-12 | 63.5 |
Find 0 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAATCTTGATGAAGAAAT+TGG | + | 32557:63210-63229 | MS.gene041610:CDS | 20.0% |
| !! | ATCAAAAAGGAGATTGTTTT+TGG | + | 32557:63340-63359 | MS.gene041610:CDS | 25.0% |
| !! | CTTCTGTTAATAAGTTTGTT+GGG | + | 32557:63460-63479 | MS.gene041610:CDS | 25.0% |
| !!! | TTTGGTTGGAATCTTATTTT+CGG | - | 32557:63258-63277 | None:intergenic | 25.0% |
| ATCATGGTTCAACATTTCAA+AGG | + | 32557:63299-63318 | MS.gene041610:CDS | 30.0% | |
| ATGAACTTCTCATTCAATCA+TGG | + | 32557:63283-63302 | MS.gene041610:CDS | 30.0% | |
| CCAACAAACTTATTAACAGA+AGG | - | 32557:63462-63481 | None:intergenic | 30.0% | |
| CCTTCTGTTAATAAGTTTGT+TGG | + | 32557:63459-63478 | MS.gene041610:CDS | 30.0% | |
| CGTAGAAAATTCACATTTGA+GGG | + | 32557:63573-63592 | MS.gene041610:CDS | 30.0% | |
| ! | ATTGTTGGAGTTGATCAAAA+AGG | + | 32557:63327-63346 | MS.gene041610:CDS | 30.0% |
| !!! | ACCACTTTTTTTCAAACCAA+CGG | - | 32557:63499-63518 | None:intergenic | 30.0% |
| AATAAGATTCCAACCAAACG+AGG | + | 32557:63260-63279 | MS.gene041610:CDS | 35.0% | |
| AGGAATTTCATGGCACTAAA+AGG | + | 32557:63414-63433 | MS.gene041610:CDS | 35.0% | |
| ATGTCTCATGAGGTTCATAT+TGG | + | 32557:63114-63133 | MS.gene041610:CDS | 35.0% | |
| ATTTCAAAGGACTCGATTGT+TGG | + | 32557:63312-63331 | MS.gene041610:CDS | 35.0% | |
| CATGAGGTTCATATTGGTAA+TGG | + | 32557:63120-63139 | MS.gene041610:CDS | 35.0% | |
| GCGTAGAAAATTCACATTTG+AGG | + | 32557:63572-63591 | MS.gene041610:CDS | 35.0% | |
| TAATGAAGGATGAACCCAAA+TGG | + | 32557:63607-63626 | MS.gene041610:CDS | 35.0% | |
| ! | TCTCTGGACAATTTTTGTCA+CGG | - | 32557:63395-63414 | None:intergenic | 35.0% |
| AATGAACCTGCACTCCATTT+GGG | - | 32557:63624-63643 | None:intergenic | 40.0% | |
| ACACCAAAATCTTGCACTCA+AGG | + | 32557:63174-63193 | MS.gene041610:CDS | 40.0% | |
| AGCCAAAATAATGTCGCTCT+CGG | - | 32557:63526-63545 | None:intergenic | 40.0% | |
| ATGAGAAGTTCATCCTCGTT+TGG | - | 32557:63276-63295 | None:intergenic | 40.0% | |
| GTGACAAAAATTGTCCAGAG+AGG | + | 32557:63394-63413 | MS.gene041610:CDS | 40.0% | |
| TAATGAACCTGCACTCCATT+TGG | - | 32557:63625-63644 | None:intergenic | 40.0% | |
| TGAAGAAATTGGATCTGCAC+CGG | + | 32557:63221-63240 | MS.gene041610:CDS | 40.0% | |
| TTGTCCAGAGAGGAATTTCA+TGG | + | 32557:63404-63423 | MS.gene041610:CDS | 40.0% | |
| ! | AATTCACATTTGAGGGTGTG+TGG | + | 32557:63580-63599 | MS.gene041610:CDS | 40.0% |
| ! | ATTTTCGGCGTTTTTACAGC+CGG | - | 32557:63243-63262 | None:intergenic | 40.0% |
| ! | TGAATTTTCTACGCATGTCG+TGG | - | 32557:63566-63585 | None:intergenic | 40.0% |
| !! | GAGGTTCATATTGGTAATGG+TGG | + | 32557:63123-63142 | MS.gene041610:CDS | 40.0% |
| !! | GCCGTTGGTTTGAAAAAAAG+TGG | + | 32557:63495-63514 | MS.gene041610:CDS | 40.0% |
| AGTGCCATGAAATTCCTCTC+TGG | - | 32557:63411-63430 | None:intergenic | 45.0% | |
| CCAAAATCTTGCACTCAAGG+TGG | + | 32557:63177-63196 | MS.gene041610:CDS | 45.0% | |
| GAAGTTCATCCTCGTTTGGT+TGG | - | 32557:63272-63291 | None:intergenic | 45.0% | |
| GGGTGTGTGGAGATTAATGA+AGG | + | 32557:63593-63612 | MS.gene041610:CDS | 45.0% | |
| TCATGGCACTAAAAGGTCGA+TGG | + | 32557:63421-63440 | MS.gene041610:CDS | 45.0% | |
| ! | CCACCTTGAGTGCAAGATTT+TGG | - | 32557:63180-63199 | None:intergenic | 45.0% |
| ! | CTCCGAGAGCGACATTATTT+TGG | + | 32557:63521-63540 | MS.gene041610:CDS | 45.0% |
| ATCTTGCACTCAAGGTGGCT+TGG | + | 32557:63182-63201 | MS.gene041610:CDS | 50.0% | |
| GATGAACCCAAATGGAGTGC+AGG | + | 32557:63615-63634 | MS.gene041610:CDS | 50.0% | |
| ! | GGGTGCTACAAACAAGCCGT+TGG | + | 32557:63480-63499 | MS.gene041610:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32557 | gene | 63114 | 63656 | 63114 | ID=MS.gene041610 |
| 32557 | mRNA | 63114 | 63656 | 63114 | ID=MS.gene041610.t1;Parent=MS.gene041610 |
| 32557 | exon | 63114 | 63656 | 63114 | ID=MS.gene041610.t1.exon1;Parent=MS.gene041610.t1 |
| 32557 | CDS | 63114 | 63656 | 63114 | ID=cds.MS.gene041610.t1;Parent=MS.gene041610.t1 |
| Gene Sequence |
| Protein sequence |
