Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041790.t1 | XP_003591924.2 | 97.9 | 436 | 9 | 0 | 1 | 436 | 1 | 436 | 2.70E-240 | 840.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041790.t1 | Q42884 | 78.1 | 438 | 92 | 2 | 1 | 434 | 1 | 438 | 1.8e-192 | 673.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041790.t1 | G7I3B2 | 97.9 | 436 | 9 | 0 | 1 | 436 | 1 | 436 | 2.0e-240 | 840.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene041790 | MS.gene09785 | PPI |
| MS.gene041790 | MS.gene007486 | PPI |
| MS.gene041790 | MS.gene007485 | PPI |
| MS.gene041790 | MS.gene061932 | PPI |
| MS.gene041790 | MS.gene007487 | PPI |
| MS.gene041790 | MS.gene22482 | PPI |
| MS.gene041790 | MS.gene018518 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041790.t1 | MTR_1g095250 | 97.936 | 436 | 9 | 0 | 1 | 436 | 1 | 436 | 0.0 | 885 |
| MS.gene041790.t1 | MTR_0368s0020 | 91.837 | 49 | 4 | 0 | 224 | 272 | 83 | 131 | 1.07e-24 | 99.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041790.t1 | AT1G48850 | 79.439 | 428 | 79 | 3 | 2 | 425 | 3 | 425 | 0.0 | 704 |
| MS.gene041790.t1 | AT1G48850 | 78.729 | 362 | 68 | 3 | 2 | 359 | 3 | 359 | 0.0 | 590 |
| MS.gene041790.t1 | AT1G48850 | 78.729 | 362 | 68 | 3 | 2 | 359 | 3 | 359 | 0.0 | 590 |
Find 128 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACATGTATCAGTCTCCTTTC+TGG | 0.119746 | 1.1:+68751137 | None:intergenic |
| CACTTCTTCTGTTTCTAATT+TGG | 0.135458 | 1.1:+68744050 | None:intergenic |
| ATCATCTTCTCTGCATATTC+AGG | 0.226636 | 1.1:+68747489 | None:intergenic |
| GCGAGATGTCATTAGCTTAT+AGG | 0.246076 | 1.1:-68750838 | MS.gene041790:CDS |
| GAGGGATCGGAGATTGGAAT+TGG | 0.269061 | 1.1:+68752334 | None:intergenic |
| TACATATGGGAACCACTTTC+GGG | 0.279701 | 1.1:-68751502 | MS.gene041790:CDS |
| CAATTTGGTAGTGGGTTTGC+AGG | 0.291322 | 1.1:-68745752 | MS.gene041790:intron |
| CACTTTCGGGTTACAACATA+TGG | 0.302913 | 1.1:-68751489 | MS.gene041790:CDS |
| CATGTATCAGTCTCCTTTCT+GGG | 0.318592 | 1.1:+68751138 | None:intergenic |
| TTCATTATTTCTCAGGGTCT+TGG | 0.321630 | 1.1:-68745848 | MS.gene041790:intron |
| GTACATATGGGAACCACTTT+CGG | 0.342196 | 1.1:-68751503 | MS.gene041790:CDS |
| TTTCCTCTTTGATCAGTATT+GGG | 0.342486 | 1.1:+68750970 | None:intergenic |
| GCTTCAAGTTTGTCAAATAC+TGG | 0.357310 | 1.1:+68745821 | None:intergenic |
| ATTTCCTCTTTGATCAGTAT+TGG | 0.358775 | 1.1:+68750969 | None:intergenic |
| CCTTTCTGGGAGTTGTAATT+CGG | 0.369236 | 1.1:+68751151 | None:intergenic |
| CGGGCAGCTGGGAGTACATA+TGG | 0.371354 | 1.1:-68751516 | MS.gene041790:CDS |
| GTGTTCACTCCCAGTCAAAA+AGG | 0.378540 | 1.1:+68745602 | None:intergenic |
| ACCATTGGAAGGGTTGCTTC+TGG | 0.385048 | 1.1:-68750643 | MS.gene041790:CDS |
| GAAATAATTAACATGAGAAT+AGG | 0.385678 | 1.1:-68745383 | MS.gene041790:CDS |
| CATGTTAATTATTTCTCCAT+TGG | 0.386598 | 1.1:+68745391 | None:intergenic |
| AATTGAAAACCCTTAGTTGC+GGG | 0.393669 | 1.1:+68745770 | None:intergenic |
| TTCTCTGCATATTCAGGGTC+TGG | 0.396812 | 1.1:+68747495 | None:intergenic |
| TTTACAGAGATTCGGGCAGC+TGG | 0.397683 | 1.1:-68751528 | MS.gene041790:intron |
| CTCATGGTCAATTAAGTCTT+CGG | 0.397981 | 1.1:+68748731 | None:intergenic |
| TAAAATACTCTCAGGAGTTT+CGG | 0.400065 | 1.1:-68751115 | MS.gene041790:CDS |
| TATGTCATTGCCCGCAACTA+AGG | 0.414702 | 1.1:-68745780 | MS.gene041790:CDS |
| GGAGGAGAGGGATCGGAGAT+TGG | 0.418731 | 1.1:+68752328 | None:intergenic |
| GGTACTTGTGGACCAATTGA+TGG | 0.418936 | 1.1:-68744133 | MS.gene041790:CDS |
| GGAAGGAGTGAGGAAGGGTT+TGG | 0.419419 | 1.1:+68752388 | None:intergenic |
| AGGGATCGGAGATTGGAATT+GGG | 0.422790 | 1.1:+68752335 | None:intergenic |
| TCATGGTCAATTAAGTCTTC+GGG | 0.427778 | 1.1:+68748732 | None:intergenic |
| GTATGGTGTCAGATCAGTTC+AGG | 0.431140 | 1.1:-68750783 | MS.gene041790:intron |
| AAACCGCTCTGGTGGGATAC+AGG | 0.446252 | 1.1:-68745532 | MS.gene041790:intron |
| GGTAGAGGCCATGGTAGCTT+TGG | 0.452564 | 1.1:-68744154 | MS.gene041790:CDS |
| TGACAAACTTGAAGCTGAGT+TGG | 0.456051 | 1.1:-68745813 | MS.gene041790:CDS |
| AGGAAGAGAAAGAATCGGTT+CGG | 0.456406 | 1.1:+68752366 | None:intergenic |
| GCAACCTATGACATGAAGTA+TGG | 0.467320 | 1.1:-68750800 | MS.gene041790:CDS |
| GTGCATGTTGTGCCATCAAT+TGG | 0.469901 | 1.1:+68744121 | None:intergenic |
| GTTGTAATTCGGCTTTGACC+TGG | 0.476712 | 1.1:+68751162 | None:intergenic |
| CAGTATTGGGCACAAATACA+TGG | 0.482313 | 1.1:+68750983 | None:intergenic |
| TTGGGCACAAATACATGGAT+TGG | 0.483473 | 1.1:+68750988 | None:intergenic |
| GGTAGACGGAGGAGAGGGAT+CGG | 0.486339 | 1.1:+68752321 | None:intergenic |
| TGTTTCTAATTTGGCTGACA+AGG | 0.491775 | 1.1:+68744059 | None:intergenic |
| GGAAGAGAAAGAATCGGTTC+GGG | 0.494003 | 1.1:+68752367 | None:intergenic |
| GGTGTTGGATGTGTCATTGA+TGG | 0.495772 | 1.1:-68751450 | MS.gene041790:CDS |
| CCGAATTACAACTCCCAGAA+AGG | 0.500551 | 1.1:-68751151 | MS.gene041790:CDS |
| ATTGGAATTGGGAGAAAGGA+AGG | 0.503710 | 1.1:+68752346 | None:intergenic |
| AGGAGTGAGGAAGGGTTTGG+TGG | 0.507069 | 1.1:+68752391 | None:intergenic |
| GAATCTCATGGAGGAGGTGT+TGG | 0.513832 | 1.1:-68751465 | MS.gene041790:CDS |
| TGAGACACTGACACTTGATC+AGG | 0.515093 | 1.1:-68748712 | MS.gene041790:intron |
| AACTCCTATATTTAGGGTGG+TGG | 0.515942 | 1.1:-68750685 | MS.gene041790:intron |
| GGAGATTGGAATTGGGAGAA+AGG | 0.519246 | 1.1:+68752342 | None:intergenic |
| GGGCAGCTGGGAGTACATAT+GGG | 0.522096 | 1.1:-68751515 | MS.gene041790:CDS |
| CATGGTAGCTTTGGTACTTG+TGG | 0.522487 | 1.1:-68744145 | MS.gene041790:CDS |
| TCATCTTCTCTGCATATTCA+GGG | 0.523950 | 1.1:+68747490 | None:intergenic |
| GGCTGAGATACCAACCTCTC+GGG | 0.524544 | 1.1:+68744547 | None:intergenic |
| ACGGAGGTAGACGGAGGAGA+GGG | 0.527565 | 1.1:+68752316 | None:intergenic |
| TTCTTGTAAATCAAAGTTCG+CGG | 0.527748 | 1.1:+68744083 | None:intergenic |
| AAGGAAGGAAGAGAAAGAAT+CGG | 0.529630 | 1.1:+68752361 | None:intergenic |
| GACGGAGGTAGACGGAGGAG+AGG | 0.531416 | 1.1:+68752315 | None:intergenic |
| TTAATTGCAGGAGTTACTAC+AGG | 0.532875 | 1.1:-68751015 | MS.gene041790:intron |
| GATACATGTAAAATACTCTC+AGG | 0.533691 | 1.1:-68751123 | MS.gene041790:CDS |
| GTTCTTGTGTATAGGAGGCC+AGG | 0.534576 | 1.1:-68751180 | MS.gene041790:intron |
| TGTCATAGGTTGCATCCGCA+TGG | 0.536508 | 1.1:+68750810 | None:intergenic |
| GACACCATACTTCATGTCAT+AGG | 0.537696 | 1.1:+68750796 | None:intergenic |
| TCTTGTAAATCAAAGTTCGC+GGG | 0.540183 | 1.1:+68744084 | None:intergenic |
| ATGTCATTGCCCGCAACTAA+GGG | 0.549586 | 1.1:-68745779 | MS.gene041790:CDS |
| TCTTCTGCTAGGGAAACCAT+TGG | 0.551202 | 1.1:-68750658 | MS.gene041790:CDS |
| GGTTCGGGAAGGAGTGAGGA+AGG | 0.551542 | 1.1:+68752382 | None:intergenic |
| AATCAGGGTGGAATTTCCAA+TGG | 0.556969 | 1.1:-68745407 | MS.gene041790:intron |
| GTGGTGGTAGATCTTCTGCT+AGG | 0.562776 | 1.1:-68750669 | MS.gene041790:CDS |
| GAAACGAGGACGGAGGTAGA+CGG | 0.563398 | 1.1:+68752307 | None:intergenic |
| CCTGTGCACTTGAGAGACAT+AGG | 0.564384 | 1.1:+68748764 | None:intergenic |
| TGCAGCTGTACCCATGGTAG+AGG | 0.565756 | 1.1:-68744169 | MS.gene041790:intron |
| CTCTGAAGCTGATATGCAAG+AGG | 0.568295 | 1.1:-68751406 | MS.gene041790:CDS |
| AGCTACCATGGCCTCTACCA+TGG | 0.568585 | 1.1:+68744158 | None:intergenic |
| ACCAGAAGCAACCCTTCCAA+TGG | 0.569596 | 1.1:+68750642 | None:intergenic |
| CCTATGTCTCTCAAGTGCAC+AGG | 0.572163 | 1.1:-68748764 | MS.gene041790:CDS |
| CAACCTCTCGGGACAACACA+GGG | 0.572864 | 1.1:+68744558 | None:intergenic |
| CATATGTTGTAACCCGAAAG+TGG | 0.573615 | 1.1:+68751490 | None:intergenic |
| ACAAGAACAAACCGCTCTGG+TGG | 0.573699 | 1.1:-68745540 | MS.gene041790:CDS |
| ACAGGGATCATGACGACCAC+GGG | 0.574091 | 1.1:+68744575 | None:intergenic |
| TGCTAGGGAAACCATTGGAA+GGG | 0.574957 | 1.1:-68750653 | MS.gene041790:CDS |
| TGTACCCATGGTAGAGGCCA+TGG | 0.577435 | 1.1:-68744163 | MS.gene041790:CDS |
| GTCATAGGTTGCATCCGCAT+GGG | 0.577755 | 1.1:+68750811 | None:intergenic |
| CTGCTAGGGAAACCATTGGA+AGG | 0.577836 | 1.1:-68750654 | MS.gene041790:CDS |
| GTGAAAGGTGATTCTGTTGG+TGG | 0.578147 | 1.1:-68747444 | MS.gene041790:CDS |
| CAACAGAATCACCTTTCACT+CGG | 0.578783 | 1.1:+68747448 | None:intergenic |
| AGGACAAGAACAAACCGCTC+TGG | 0.581332 | 1.1:-68745543 | MS.gene041790:CDS |
| TGGTGGTAGATCTTCTGCTA+GGG | 0.582067 | 1.1:-68750668 | MS.gene041790:CDS |
| ACAACATATGGAGAATCTCA+TGG | 0.584104 | 1.1:-68751477 | MS.gene041790:CDS |
| TTATATAGATCAAAATGGAA+AGG | 0.585581 | 1.1:-68745568 | MS.gene041790:CDS |
| GATCAAGTGTCAGTGTCTCA+TGG | 0.586576 | 1.1:+68748715 | None:intergenic |
| ATTGATGCTGTCCGAGTGAA+AGG | 0.587265 | 1.1:-68747459 | MS.gene041790:CDS |
| GCTACCATGGCCTCTACCAT+GGG | 0.587349 | 1.1:+68744159 | None:intergenic |
| TGCATATCAGCTTCAGAGAG+AGG | 0.589694 | 1.1:+68751411 | None:intergenic |
| TTACAGAGATTCGGGCAGCT+GGG | 0.592616 | 1.1:-68751527 | MS.gene041790:intron |
| GCTTCAGAGAGAGGGAGGCG+AGG | 0.596196 | 1.1:+68751420 | None:intergenic |
| GCATATCAGCTTCAGAGAGA+GGG | 0.596658 | 1.1:+68751412 | None:intergenic |
| GAAGCAATCTACAGTGACTC+GGG | 0.598566 | 1.1:-68744622 | MS.gene041790:CDS |
| GTGCCCAATACTGATCAAAG+AGG | 0.603337 | 1.1:-68750973 | MS.gene041790:CDS |
| CACAAGTACCAAAGCTACCA+TGG | 0.605561 | 1.1:+68744146 | None:intergenic |
| CACAGGGATCATGACGACCA+CGG | 0.605920 | 1.1:+68744574 | None:intergenic |
| TGAACTTGCAGCTGTACCCA+TGG | 0.607274 | 1.1:-68744175 | MS.gene041790:intron |
| TATGGAGAATCTCATGGAGG+AGG | 0.607485 | 1.1:-68751471 | MS.gene041790:CDS |
| TAGGTTGCATCCGCATGGGA+AGG | 0.607769 | 1.1:+68750815 | None:intergenic |
| ATACTCTCAGGAGTTTCGGA+AGG | 0.610665 | 1.1:-68751111 | MS.gene041790:intron |
| AGAAGCAATCTACAGTGACT+CGG | 0.610730 | 1.1:-68744623 | MS.gene041790:CDS |
| CCAACCTCTCGGGACAACAC+AGG | 0.611393 | 1.1:+68744557 | None:intergenic |
| AGCTTATAGGCCTTCCCATG+CGG | 0.616697 | 1.1:-68750825 | MS.gene041790:CDS |
| AATCGGTTCGGGAAGGAGTG+AGG | 0.619223 | 1.1:+68752378 | None:intergenic |
| GGTGGAGATCGAAGAAGCCA+TGG | 0.622096 | 1.1:+68752409 | None:intergenic |
| GTTCGGGAAGGAGTGAGGAA+GGG | 0.624145 | 1.1:+68752383 | None:intergenic |
| AAATTGAAAACCCTTAGTTG+CGG | 0.624146 | 1.1:+68745769 | None:intergenic |
| TCAGAGAGAGGGAGGCGAGG+AGG | 0.627324 | 1.1:+68751423 | None:intergenic |
| GAGAAAGAATCGGTTCGGGA+AGG | 0.628737 | 1.1:+68752371 | None:intergenic |
| TGCAAGAGGATCTTGACAGA+AGG | 0.632234 | 1.1:-68751392 | MS.gene041790:intron |
| CAAGAACAAACCGCTCTGGT+GGG | 0.632701 | 1.1:-68745539 | MS.gene041790:CDS |
| CTTACTGTAATAGTCGATGT+CGG | 0.643905 | 1.1:+68745353 | None:intergenic |
| TATCAGCTTCAGAGAGAGGG+AGG | 0.644707 | 1.1:+68751415 | None:intergenic |
| TAGATCAAAATGGAAAGGTG+AGG | 0.648351 | 1.1:-68745563 | MS.gene041790:CDS |
| CGAGTGAAAGGTGATTCTGT+TGG | 0.682023 | 1.1:-68747447 | MS.gene041790:CDS |
| GAAACAGAGCTCATAGCCCG+TGG | 0.694287 | 1.1:-68744591 | MS.gene041790:CDS |
| ACGAGGACGGAGGTAGACGG+AGG | 0.699102 | 1.1:+68752310 | None:intergenic |
| GTGTTGTAACATGCATTGTG+AGG | 0.723456 | 1.1:-68747422 | MS.gene041790:CDS |
| GATCCCTGTGTTGTCCCGAG+AGG | 0.731256 | 1.1:-68744561 | MS.gene041790:intron |
| ACATATGGAGAATCTCATGG+AGG | 0.763629 | 1.1:-68751474 | MS.gene041790:CDS |
| ATACCTGTATCCCACCAGAG+CGG | 0.816288 | 1.1:+68745529 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 68744041 | 68752429 | 68744041 | ID=MS.gene041790 |
| chr1.1 | mRNA | 68744041 | 68752429 | 68744041 | ID=MS.gene041790.t1;Parent=MS.gene041790 |
| chr1.1 | exon | 68752291 | 68752429 | 68752291 | ID=MS.gene041790.t1.exon1;Parent=MS.gene041790.t1 |
| chr1.1 | CDS | 68752291 | 68752429 | 68752291 | ID=cds.MS.gene041790.t1;Parent=MS.gene041790.t1 |
| chr1.1 | exon | 68751393 | 68751543 | 68751393 | ID=MS.gene041790.t1.exon2;Parent=MS.gene041790.t1 |
| chr1.1 | CDS | 68751393 | 68751543 | 68751393 | ID=cds.MS.gene041790.t1;Parent=MS.gene041790.t1 |
| chr1.1 | exon | 68751112 | 68751188 | 68751112 | ID=MS.gene041790.t1.exon3;Parent=MS.gene041790.t1 |
| chr1.1 | CDS | 68751112 | 68751188 | 68751112 | ID=cds.MS.gene041790.t1;Parent=MS.gene041790.t1 |
| chr1.1 | exon | 68750969 | 68751027 | 68750969 | ID=MS.gene041790.t1.exon4;Parent=MS.gene041790.t1 |
| chr1.1 | CDS | 68750969 | 68751027 | 68750969 | ID=cds.MS.gene041790.t1;Parent=MS.gene041790.t1 |
| chr1.1 | exon | 68750784 | 68750867 | 68750784 | ID=MS.gene041790.t1.exon5;Parent=MS.gene041790.t1 |
| chr1.1 | CDS | 68750784 | 68750867 | 68750784 | ID=cds.MS.gene041790.t1;Parent=MS.gene041790.t1 |
| chr1.1 | exon | 68750600 | 68750692 | 68750600 | ID=MS.gene041790.t1.exon6;Parent=MS.gene041790.t1 |
| chr1.1 | CDS | 68750600 | 68750692 | 68750600 | ID=cds.MS.gene041790.t1;Parent=MS.gene041790.t1 |
| chr1.1 | exon | 68748713 | 68748793 | 68748713 | ID=MS.gene041790.t1.exon7;Parent=MS.gene041790.t1 |
| chr1.1 | CDS | 68748713 | 68748793 | 68748713 | ID=cds.MS.gene041790.t1;Parent=MS.gene041790.t1 |
| chr1.1 | exon | 68747410 | 68747541 | 68747410 | ID=MS.gene041790.t1.exon8;Parent=MS.gene041790.t1 |
| chr1.1 | CDS | 68747410 | 68747541 | 68747410 | ID=cds.MS.gene041790.t1;Parent=MS.gene041790.t1 |
| chr1.1 | exon | 68745753 | 68745855 | 68745753 | ID=MS.gene041790.t1.exon9;Parent=MS.gene041790.t1 |
| chr1.1 | CDS | 68745753 | 68745855 | 68745753 | ID=cds.MS.gene041790.t1;Parent=MS.gene041790.t1 |
| chr1.1 | exon | 68745533 | 68745627 | 68745533 | ID=MS.gene041790.t1.exon10;Parent=MS.gene041790.t1 |
| chr1.1 | CDS | 68745533 | 68745627 | 68745533 | ID=cds.MS.gene041790.t1;Parent=MS.gene041790.t1 |
| chr1.1 | exon | 68745358 | 68745423 | 68745358 | ID=MS.gene041790.t1.exon11;Parent=MS.gene041790.t1 |
| chr1.1 | CDS | 68745358 | 68745423 | 68745358 | ID=cds.MS.gene041790.t1;Parent=MS.gene041790.t1 |
| chr1.1 | exon | 68744562 | 68744646 | 68744562 | ID=MS.gene041790.t1.exon12;Parent=MS.gene041790.t1 |
| chr1.1 | CDS | 68744562 | 68744646 | 68744562 | ID=cds.MS.gene041790.t1;Parent=MS.gene041790.t1 |
| chr1.1 | exon | 68744041 | 68744186 | 68744041 | ID=MS.gene041790.t1.exon13;Parent=MS.gene041790.t1 |
| chr1.1 | CDS | 68744041 | 68744186 | 68744041 | ID=cds.MS.gene041790.t1;Parent=MS.gene041790.t1 |
| Gene Sequence |
| Protein sequence |
