AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene041990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041990.t1 MTR_5g073210 96.786 560 17 1 1 559 1 560 0.0 1119
MS.gene041990.t1 MTR_5g083360 60.317 567 217 3 1 559 1 567 0.0 738
MS.gene041990.t1 MTR_3g071890 57.865 534 217 4 3 534 12 539 0.0 656
MS.gene041990.t1 MTR_5g020620 52.491 562 253 6 3 559 9 561 0.0 600
MS.gene041990.t1 MTR_2g008330 48.239 568 281 7 2 559 15 579 0.0 582
MS.gene041990.t1 MTR_5g020600 51.681 565 254 7 3 559 13 566 0.0 581
MS.gene041990.t1 MTR_7g060460 51.332 563 248 8 16 559 26 581 0.0 571
MS.gene041990.t1 MTR_7g062250 51.670 569 254 10 7 559 15 578 0.0 565
MS.gene041990.t1 MTR_7g062310 50.727 550 252 6 25 559 48 593 0.0 565
MS.gene041990.t1 MTR_7g058690 50.000 576 259 10 4 559 14 580 0.0 560
MS.gene041990.t1 MTR_7g458880 49.914 579 261 10 1 559 11 580 0.0 560
MS.gene041990.t1 MTR_5g069680 48.940 566 269 8 4 559 3 558 0.0 559
MS.gene041990.t1 MTR_4g015120 49.821 558 266 6 8 559 8 557 0.0 557
MS.gene041990.t1 MTR_3g101635 49.628 538 263 5 11 542 14 549 0.0 550
MS.gene041990.t1 MTR_3g462760 50.547 548 255 7 17 559 20 556 0.0 547
MS.gene041990.t1 MTR_3g101640 49.541 545 266 6 22 559 29 571 0.0 547
MS.gene041990.t1 MTR_4g064530 50.179 560 258 12 1 547 22 573 0.0 535
MS.gene041990.t1 MTR_7g065970 49.472 568 271 8 4 559 7 570 0.0 532
MS.gene041990.t1 MTR_7g065980 45.581 577 290 10 1 559 1 571 0.0 531
MS.gene041990.t1 MTR_4g019225 48.263 547 268 6 26 559 28 572 0.0 528
MS.gene041990.t1 MTR_5g081810 48.704 540 258 10 26 559 3 529 0.0 527
MS.gene041990.t1 MTR_8g027375 43.604 555 302 7 12 559 21 571 2.07e-173 503
MS.gene041990.t1 MTR_4g064530 49.540 543 252 13 1 530 22 555 7.63e-173 501
MS.gene041990.t1 MTR_8g091390 30.986 568 332 18 16 550 24 564 6.30e-67 227
MS.gene041990.t1 MTR_3g078700 31.373 561 301 21 4 534 9 515 3.65e-66 224
MS.gene041990.t1 MTR_5g081790 44.203 276 136 9 248 517 157 420 1.45e-63 214
MS.gene041990.t1 MTR_5g081790 45.098 153 77 1 18 163 18 170 1.53e-41 155
MS.gene041990.t1 MTR_3g078740 30.268 598 336 22 4 549 9 577 8.56e-63 217
MS.gene041990.t1 MTR_3g078730 29.840 563 334 19 4 549 9 527 2.68e-57 201
MS.gene041990.t1 MTR_3g008580 27.256 554 358 16 5 541 8 533 2.28e-45 169
MS.gene041990.t1 MTR_4g101650 26.889 569 378 16 4 559 6 549 2.66e-45 169
MS.gene041990.t1 MTR_1g090707 28.281 541 327 19 38 559 38 536 1.04e-44 167
MS.gene041990.t1 MTR_4g075690 28.098 573 341 21 8 559 10 532 7.23e-43 161
MS.gene041990.t1 MTR_3g083400 26.381 561 341 16 4 541 6 517 1.77e-42 160
MS.gene041990.t1 MTR_3g083430 27.289 557 341 19 4 541 6 517 2.42e-42 160
MS.gene041990.t1 MTR_3g083440 27.289 557 341 19 4 541 6 517 4.12e-42 159
MS.gene041990.t1 MTR_3g083390 27.289 557 341 19 4 541 6 517 4.33e-42 159
MS.gene041990.t1 MTR_4g049390 27.768 569 351 18 6 559 7 530 5.09e-42 159
MS.gene041990.t1 MTR_4g083190 27.555 548 326 18 2 525 3 503 2.55e-41 157
MS.gene041990.t1 MTR_3g115050 28.397 574 351 20 1 559 5 533 3.32e-41 156
MS.gene041990.t1 MTR_3g083450 27.324 527 338 16 25 541 27 518 5.17e-41 156
MS.gene041990.t1 MTR_8g479380 27.514 527 334 17 8 525 10 497 4.48e-40 153
MS.gene041990.t1 MTR_4g083210 26.345 539 329 19 8 525 6 497 4.60e-40 153
MS.gene041990.t1 MTR_4g048350 29.032 527 317 19 45 559 48 529 7.23e-40 152
MS.gene041990.t1 MTR_5g018270 26.871 521 323 19 17 525 22 496 9.45e-39 149
MS.gene041990.t1 MTR_3g083410 26.667 525 314 15 40 541 5 481 1.02e-38 149
MS.gene041990.t1 MTR_8g479390 26.419 511 333 16 21 525 26 499 1.43e-38 149
MS.gene041990.t1 MTR_1g026410 26.408 568 364 15 4 559 7 532 1.93e-38 149
MS.gene041990.t1 MTR_2g088080 28.893 533 322 18 39 559 46 533 2.12e-38 148
MS.gene041990.t1 MTR_8g073840 40.500 200 79 5 17 214 23 184 7.67e-37 139
MS.gene041990.t1 MTR_4g075690 28.953 487 276 21 8 473 10 447 7.06e-35 137
MS.gene041990.t1 MTR_7g051440 26.488 521 326 16 25 524 30 514 9.38e-35 139
MS.gene041990.t1 MTR_4g132320 27.385 566 355 20 7 559 12 534 2.85e-32 130
MS.gene041990.t1 MTR_2g088080 28.395 486 302 17 81 559 6 452 2.76e-29 121
MS.gene041990.t1 MTR_7g058670 58.025 81 33 1 81 160 13 93 3.07e-26 103
MS.gene041990.t1 MTR_3g079660 37.363 91 52 2 469 559 5 90 9.29e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041990.t1 AT2G30210 69.982 563 160 2 5 559 9 570 0.0 829
MS.gene041990.t1 AT5G07130 65.263 570 186 5 1 559 1 569 0.0 763
MS.gene041990.t1 AT5G05390 60.428 561 215 5 3 559 8 565 0.0 720
MS.gene041990.t1 AT2G40370 58.160 576 223 4 2 559 5 580 0.0 715
MS.gene041990.t1 AT5G07130 67.835 485 144 5 86 559 1 484 0.0 667
MS.gene041990.t1 AT5G07130 67.835 485 144 5 86 559 1 484 0.0 667
MS.gene041990.t1 AT5G09360 49.378 563 273 7 1 559 15 569 0.0 577
MS.gene041990.t1 AT2G29130 52.182 573 253 9 4 559 5 573 0.0 577
MS.gene041990.t1 AT5G03260 50.980 561 259 7 4 559 8 557 0.0 566
MS.gene041990.t1 AT5G60020 51.607 560 238 10 25 559 26 577 0.0 564
MS.gene041990.t1 AT5G60020 52.364 550 229 10 35 559 4 545 0.0 562
MS.gene041990.t1 AT2G38080 49.559 567 269 8 1 559 1 558 0.0 554
MS.gene041990.t1 AT5G58910 48.940 566 270 6 4 559 11 567 0.0 545
MS.gene041990.t1 AT5G58910 48.940 566 269 7 4 559 11 566 0.0 543
MS.gene041990.t1 AT5G01190 49.643 560 269 6 5 559 7 558 0.0 537
MS.gene041990.t1 AT2G46570 45.730 562 294 7 5 559 12 569 0.0 525
MS.gene041990.t1 AT5G58910 49.719 533 243 6 37 559 6 523 0.0 523
MS.gene041990.t1 AT3G09220 49.275 552 258 9 4 542 8 550 0.0 523
MS.gene041990.t1 AT5G01190 51.145 524 244 5 41 559 28 544 0.0 522
MS.gene041990.t1 AT1G18140 47.257 565 270 9 17 559 23 581 2.23e-177 513
MS.gene041990.t1 AT5G60020 51.544 518 218 10 25 517 26 535 1.27e-176 510
MS.gene041990.t1 AT5G01040 45.357 560 287 7 11 559 15 566 2.68e-172 500
MS.gene041990.t1 AT5G48100 45.149 536 280 9 24 549 22 553 1.36e-168 490
MS.gene041990.t1 AT5G01050 44.840 562 289 7 11 559 15 568 3.71e-167 487
MS.gene041990.t1 AT2G46570 45.979 485 252 6 81 559 4 484 1.09e-156 457
MS.gene041990.t1 AT3G09220 49.455 459 212 8 97 542 2 453 3.68e-149 437
MS.gene041990.t1 AT5G21100 31.461 534 302 18 39 538 39 542 3.92e-69 233
MS.gene041990.t1 AT4G39830 31.171 555 303 21 39 549 51 570 5.77e-67 228
MS.gene041990.t1 AT5G21105 30.823 571 323 22 5 537 22 558 6.27e-65 222
MS.gene041990.t1 AT5G21105 30.309 551 312 22 5 517 22 538 1.97e-55 196
MS.gene041990.t1 AT4G39830 30.118 508 277 20 85 549 1 473 1.79e-49 178
MS.gene041990.t1 AT4G39830 30.159 504 274 20 89 549 7 475 8.54e-49 176
MS.gene041990.t1 AT1G75790 28.037 535 329 15 1 519 1 495 3.11e-48 176
MS.gene041990.t1 AT4G25240 29.072 571 358 18 4 559 12 550 8.73e-47 173
MS.gene041990.t1 AT4G25240 29.072 571 358 18 4 559 12 550 8.73e-47 173
MS.gene041990.t1 AT4G12420 27.612 536 350 15 5 527 6 516 3.10e-45 169
MS.gene041990.t1 AT4G12420 27.612 536 350 15 5 527 6 516 3.10e-45 169
MS.gene041990.t1 AT4G39830 29.423 503 266 19 89 549 7 462 6.89e-45 165
MS.gene041990.t1 AT5G66920 27.568 555 328 17 25 559 38 538 8.71e-44 164
MS.gene041990.t1 AT1G21850 29.104 536 311 19 8 525 12 496 8.07e-43 161
MS.gene041990.t1 AT5G51480 26.738 561 370 17 14 559 16 550 2.47e-42 160
MS.gene041990.t1 AT5G48450 26.834 559 356 19 8 556 13 528 5.54e-42 159
MS.gene041990.t1 AT5G48450 27.013 559 355 19 8 556 13 528 9.14e-42 159
MS.gene041990.t1 AT1G21860 26.894 528 334 16 8 525 11 496 3.58e-40 153
MS.gene041990.t1 AT4G22010 27.387 555 362 15 11 559 11 530 9.24e-40 152
MS.gene041990.t1 AT1G76160 27.419 558 360 17 8 559 12 530 2.02e-39 151
MS.gene041990.t1 AT1G41830 26.018 565 367 16 3 559 10 531 4.30e-38 147
MS.gene041990.t1 AT4G28090 25.603 539 333 15 6 524 9 499 1.35e-37 146
MS.gene041990.t1 AT2G23630 29.412 527 321 19 42 559 53 537 5.57e-36 142
MS.gene041990.t1 AT2G23630 29.412 527 321 19 42 559 48 532 6.39e-36 141
MS.gene041990.t1 AT1G55570 25.599 543 328 16 4 519 7 500 7.64e-36 141
MS.gene041990.t1 AT4G38420 26.022 538 337 18 6 524 9 504 3.16e-34 137
MS.gene041990.t1 AT4G37160 27.402 562 367 17 2 559 10 534 1.01e-33 135
MS.gene041990.t1 AT5G21105 30.205 341 202 11 209 537 51 367 5.33e-33 130
MS.gene041990.t1 AT3G13390 25.479 522 311 17 25 519 29 499 9.36e-32 129
MS.gene041990.t1 AT1G55560 25.281 534 339 19 4 519 5 496 8.77e-31 126
MS.gene041990.t1 AT3G13400 25.234 535 338 20 4 519 6 497 1.11e-29 123
MS.gene041990.t1 AT3G13400 25.234 535 338 20 4 519 6 497 1.11e-29 123
MS.gene041990.t1 AT4G38420 25.781 512 319 18 6 498 9 478 3.43e-29 121
MS.gene041990.t1 AT4G38420 31.047 277 178 7 6 278 9 276 9.81e-29 119
MS.gene041990.t1 AT4G37160 28.990 307 197 6 2 305 10 298 9.18e-26 110

Find 149 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCGAAACACTTTAATTT+TGG 0.112254 5.4:-60965447 None:intergenic
CCTTGACACCTTGGACTATT+AGG 0.188458 5.4:-60965524 None:intergenic
TTTATAGTTGGTTCAGGTTT+TGG 0.199584 5.4:+60965845 MS.gene041990:CDS
GCCACCATAGTGTTCCTTCT+TGG 0.231177 5.4:-60964096 None:intergenic
CGAAGGATACTCACCGTAAA+TGG 0.256488 5.4:+60963746 MS.gene041990:CDS
GTCTTGAAGGTGGTCTATTT+TGG 0.257321 5.4:-60965349 None:intergenic
ATTGTTGTGAATCAGGAATT+TGG 0.267922 5.4:+60966271 MS.gene041990:intron
AAGTTGTTCAAGTTGAAATA+TGG 0.300472 5.4:+60965740 MS.gene041990:CDS
AGAGAATTTCCCGTTCTTCT+TGG 0.306078 5.4:+60964211 MS.gene041990:CDS
AATGGTAAAGCCGGCAAAAT+AGG 0.318344 5.4:-60965371 None:intergenic
TGTATGCAACAGAAACATTT+GGG 0.327517 5.4:-60964756 None:intergenic
GCTTACTACAATGGTGTTCC+TGG 0.329058 5.4:+60965623 MS.gene041990:CDS
CATTTCTTTATAGTTGGTTC+AGG 0.332092 5.4:+60965839 MS.gene041990:CDS
CTCCATAGACAGTGGCTCTT+AGG 0.334059 5.4:-60964135 None:intergenic
AACGTAGCAGGATCAGTTCT+TGG 0.335909 5.4:-60965878 None:intergenic
AAGGTCCAGCATTTGTTACT+TGG 0.341260 5.4:-60963820 None:intergenic
ACATAGATTCACATCTCAAT+TGG 0.345519 5.4:+60966307 MS.gene041990:CDS
GCCAGGAAACTGTCCATTTA+CGG 0.347312 5.4:-60963759 None:intergenic
ATGAATTATACCTGGATTAT+CGG 0.349140 5.4:-60965975 None:intergenic
CCGTCCCTCGCTTCGATCGT+TGG 0.351478 5.4:-60963782 None:intergenic
TTCCTAAGAGCCACTGTCTA+TGG 0.354270 5.4:+60964133 MS.gene041990:CDS
GGGTTTGGCAACAGCACTTT+TGG 0.375326 5.4:+60966329 MS.gene041990:CDS
TTCACATCTCAATTGGGGTT+TGG 0.376065 5.4:+60966314 MS.gene041990:CDS
GGGTTCCATTTCTTTATAGT+TGG 0.378042 5.4:+60965833 MS.gene041990:CDS
CATAGATTCACATCTCAATT+GGG 0.380545 5.4:+60966308 MS.gene041990:CDS
CCATTTGGTCCTTGACACCT+TGG 0.385637 5.4:-60965533 None:intergenic
GCTGCAAATCTTGTTCCATT+TGG 0.386553 5.4:-60965548 None:intergenic
TGCATTAAGGAAGTTGTTCT+AGG 0.389050 5.4:-60965599 None:intergenic
ACCGTAAATGGACAGTTTCC+TGG 0.396378 5.4:+60963758 MS.gene041990:CDS
ATGGGTCGCCATTCGTTTCA+AGG 0.412325 5.4:+60965952 MS.gene041990:CDS
CCACCTGTGAGGAATACTAT+TGG 0.412353 5.4:+60965914 MS.gene041990:CDS
CGTCCCTCGCTTCGATCGTT+GGG 0.413929 5.4:-60963781 None:intergenic
TGTGCAACAGGCATGGGCTT+AGG 0.415052 5.4:+60963978 MS.gene041990:intron
GTGTATGCAACAGAAACATT+TGG 0.416717 5.4:-60964757 None:intergenic
GCATTCACATCAAGGATTAG+AGG 0.421253 5.4:+60965419 MS.gene041990:CDS
TTATTGTTCACTGTAGGGTT+AGG 0.421636 5.4:+60965482 MS.gene041990:CDS
GAATGATTTCAAACCTTGAC+TGG 0.425857 5.4:-60964815 None:intergenic
CTGAACCAACTATAAAGAAA+TGG 0.426203 5.4:-60965838 None:intergenic
GTTGTCAAATGCAGCATTCA+TGG 0.428789 5.4:-60965300 None:intergenic
TGCTGCCATGTAGTATCGGC+CGG 0.431892 5.4:-60965261 None:intergenic
ATGCAGCATTCATGGCGGTT+TGG 0.436299 5.4:-60965292 None:intergenic
GAGGTATCACTGACTGTAAT+GGG 0.438774 5.4:-60966368 None:intergenic
TTGAACTGTATAGGAGGAAC+AGG 0.439285 5.4:-60965668 None:intergenic
GATCACCCTATGCATGTTCA+TGG 0.442341 5.4:+60965812 MS.gene041990:CDS
GAAATGTTCCTAGGGGTTTG+TGG 0.445135 5.4:+60965699 MS.gene041990:CDS
TCTCTAGTTGGCTTAGAGAA+AGG 0.447045 5.4:-60964193 None:intergenic
TGAAGATTACCAAGAAGAAC+GGG 0.448241 5.4:-60964220 None:intergenic
AGAACGGGAAATTCTCTAGT+TGG 0.448541 5.4:-60964205 None:intergenic
CTCGCTTCGATCGTTGGGCC+AGG 0.448652 5.4:-60963776 None:intergenic
ATTACACAGGAAATGTTCCT+AGG 0.452764 5.4:+60965690 MS.gene041990:CDS
CGTTTCAAGGCCGATAATCC+AGG 0.464778 5.4:+60965965 MS.gene041990:CDS
CCTGTGTAATTGAACTGTAT+AGG 0.466472 5.4:-60965677 None:intergenic
ATCTCCTGGTTGACCATTCA+CGG 0.467009 5.4:-60964779 None:intergenic
CTGCAAAACAATTTGAACAT+TGG 0.467774 5.4:-60965765 None:intergenic
GTTTGTCTTAAGAGAGCCAT+TGG 0.471023 5.4:-60964715 None:intergenic
CCAAGGTGTCAAGGACCAAA+TGG 0.477385 5.4:+60965533 MS.gene041990:CDS
GCCGGCAAAATAGGTCTTGA+AGG 0.477836 5.4:-60965362 None:intergenic
GTGAGTTTATTGTTCACTGT+AGG 0.484623 5.4:+60965476 MS.gene041990:CDS
TAACTTCCACCTGGTTGGAT+TGG 0.489901 5.4:-60964049 None:intergenic
ATTGTAGTAAGCTTGCATTA+AGG 0.490056 5.4:-60965612 None:intergenic
TCTTAAGAGAATTGTTTCTC+CGG 0.491448 5.4:-60965075 None:intergenic
TCGACAAGGTTGAACGTAGC+AGG 0.491890 5.4:-60965890 None:intergenic
GTATCGGCCGGGTGGTTGAT+CGG 0.496922 5.4:-60965249 None:intergenic
TGAGTTTATTGTTCACTGTA+GGG 0.502703 5.4:+60965477 MS.gene041990:CDS
AACACTAGAGTCCTCATGAT+AGG 0.504563 5.4:+60965194 MS.gene041990:CDS
GGAGGTATCACTGACTGTAA+TGG 0.504892 5.4:-60966369 None:intergenic
TTGGAACACCTCCTGGTGGA+TGG 0.505700 5.4:+60965933 MS.gene041990:CDS
AATACTATTGGAACACCTCC+TGG 0.507986 5.4:+60965926 MS.gene041990:CDS
TCTGTTGCATACACCGTGAA+TGG 0.509061 5.4:+60964766 MS.gene041990:CDS
CTCTATCGATGTTCCAGTCA+AGG 0.512402 5.4:+60964802 MS.gene041990:CDS
TGGAACACCTCCTGGTGGAT+GGG 0.516621 5.4:+60965934 MS.gene041990:CDS
AAAACCAAGAAGGAACACTA+TGG 0.519751 5.4:+60964092 MS.gene041990:CDS
CAGTGGATGGAGATGTTGTA+AGG 0.523772 5.4:-60963839 None:intergenic
TTGAAGATTACCAAGAAGAA+CGG 0.530236 5.4:-60964221 None:intergenic
CTGCAACTGCATTCACATCA+AGG 0.532127 5.4:+60965411 MS.gene041990:CDS
GTGATCCAAGTAACAAATGC+TGG 0.533819 5.4:+60963815 MS.gene041990:CDS
CGCGTGCTGCCATGTAGTAT+CGG 0.537076 5.4:-60965265 None:intergenic
TGAGGTAGATCTGGTGGTGG+AGG 0.538755 5.4:-60966387 None:intergenic
GGACATTGTGTCACATAACT+AGG 0.539105 5.4:-60964028 None:intergenic
TGGAACCCATGAACATGCAT+AGG 0.539781 5.4:-60965818 None:intergenic
TTTACAATTCAAAACCAAGA+AGG 0.543934 5.4:+60964082 MS.gene041990:CDS
GAACATCGATAGAGATCTCC+TGG 0.547001 5.4:-60964793 None:intergenic
TGTTTCATTTGCTGCAGCAG+AGG 0.548223 5.4:+60963577 MS.gene041990:CDS
ATATTTCAACTTGAACAACT+TGG 0.548915 5.4:-60965738 None:intergenic
CGATGATAGTTACCAGTGGA+TGG 0.549490 5.4:-60963852 None:intergenic
TATCATGAGGACTCTAGTGT+TGG 0.554372 5.4:-60965192 None:intergenic
TTGAGAAATCCATGGGCAGA+TGG 0.554935 5.4:+60964004 MS.gene041990:CDS
GTCACAGCCGATCAACCACC+CGG 0.555234 5.4:+60965242 MS.gene041990:CDS
CTAGGTGTCCACAAACCCCT+AGG 0.555841 5.4:-60965707 None:intergenic
ACCACCCGGCCGATACTACA+TGG 0.559330 5.4:+60965256 MS.gene041990:CDS
ATGTGTAACTTCCACCTGGT+TGG 0.559503 5.4:-60964054 None:intergenic
TTACACAGGAAATGTTCCTA+GGG 0.560206 5.4:+60965691 MS.gene041990:CDS
CCTAATAGTCCAAGGTGTCA+AGG 0.569314 5.4:+60965524 MS.gene041990:CDS
GCTGCCATGTAGTATCGGCC+GGG 0.571411 5.4:-60965260 None:intergenic
TAGCATTGAGGTAGATCTGG+TGG 0.573248 5.4:-60966393 None:intergenic
CGAATGGCGACCCATCCACC+AGG 0.580203 5.4:-60965944 None:intergenic
ATGGTTTGATAGAGATCCAA+TGG 0.580804 5.4:+60964699 MS.gene041990:CDS
GGTTTGTGGACACCTAGAAA+AGG 0.582031 5.4:+60965713 MS.gene041990:CDS
TACTAGTATAGTAACAATTG+AGG 0.584246 5.4:+60965790 MS.gene041990:CDS
CGGTATGTGTAACTTCCACC+TGG 0.585408 5.4:-60964058 None:intergenic
ATGAGGACTCTAGTGTTGGA+AGG 0.587715 5.4:-60965188 None:intergenic
GGCATAGACTTGAAAACGTA+CGG 0.589109 5.4:-60965054 None:intergenic
GTGGTTGATCGGCTGTGACA+AGG 0.589904 5.4:-60965238 None:intergenic
TTAATGCAAGCTTACTACAA+TGG 0.590912 5.4:+60965614 MS.gene041990:CDS
TTCAAACTGCAACAGTGAAG+AGG 0.591115 5.4:+60963711 MS.gene041990:CDS
ATCACCCTATGCATGTTCAT+GGG 0.594471 5.4:+60965813 MS.gene041990:CDS
ATAGATTCACATCTCAATTG+GGG 0.596176 5.4:+60966309 MS.gene041990:CDS
CCAACGATCGAAGCGAGGGA+CGG 0.596308 5.4:+60963782 MS.gene041990:CDS
TTAAGACAAACACAGTTCAC+CGG 0.596905 5.4:+60964727 MS.gene041990:CDS
GCACTAATCCTAATAGTCCA+AGG 0.603359 5.4:+60965516 MS.gene041990:CDS
TGGCCCAACGATCGAAGCGA+GGG 0.604197 5.4:+60963778 MS.gene041990:CDS
AGGACATTGATTGTTTGACC+TGG 0.607085 5.4:-60965218 None:intergenic
AAACACTTACAACAAACTGA+TGG 0.608462 5.4:-60963607 None:intergenic
CATTGAGGTAGATCTGGTGG+TGG 0.610540 5.4:-60966390 None:intergenic
TTAGGATGTTGAGAAATCCA+TGG 0.611446 5.4:+60963996 MS.gene041990:CDS
TTTGACCTGGTCCTATCATG+AGG 0.614547 5.4:-60965205 None:intergenic
GTGTAATTGAACTGTATAGG+AGG 0.616090 5.4:-60965674 None:intergenic
CTGGCCCAACGATCGAAGCG+AGG 0.616399 5.4:+60963777 MS.gene041990:CDS
GGAACCCATGAACATGCATA+GGG 0.618003 5.4:-60965817 None:intergenic
CCTATACAGTTCAATTACAC+AGG 0.618962 5.4:+60965677 MS.gene041990:CDS
CTATGGTGGCATGCTCACAC+TGG 0.620025 5.4:+60964109 MS.gene041990:CDS
TAGGATGTTGAGAAATCCAT+GGG 0.620108 5.4:+60963997 MS.gene041990:CDS
ATTATCGGCCTTGAAACGAA+TGG 0.624949 5.4:-60965960 None:intergenic
CCAATAGTATTCCTCACAGG+TGG 0.626541 5.4:-60965914 None:intergenic
TAACTAGGTCCATCTGCCCA+TGG 0.627031 5.4:-60964013 None:intergenic
CTTACAACATCTCCATCCAC+TGG 0.628162 5.4:+60963840 MS.gene041990:CDS
TCCTCACAGGTGGATCGACA+AGG 0.628411 5.4:-60965904 None:intergenic
AGAGTCCTCATGATAGGACC+AGG 0.629037 5.4:+60965200 MS.gene041990:CDS
GTTCCAATAGTATTCCTCAC+AGG 0.630506 5.4:-60965917 None:intergenic
TACACCGTGAATGGTCAACC+AGG 0.630802 5.4:+60964775 MS.gene041990:CDS
TTGCAACAACAGTCATTCTG+TGG 0.631813 5.4:-60965145 None:intergenic
AACTGTATAGGAGGAACAGG+AGG 0.636960 5.4:-60965665 None:intergenic
CAGAATGACTGTTGTTGCAA+CGG 0.639749 5.4:+60965148 MS.gene041990:CDS
ACACAATGTCCAATCCAACC+AGG 0.647850 5.4:+60964040 MS.gene041990:CDS
TACACAGGAAATGTTCCTAG+GGG 0.663460 5.4:+60965692 MS.gene041990:CDS
TGATGTGAATGCAGTTGCAG+TGG 0.665699 5.4:-60965408 None:intergenic
ACCAAGAAGGAACACTATGG+TGG 0.666226 5.4:+60964095 MS.gene041990:CDS
AATGAAAGCTCCATAGACAG+TGG 0.677360 5.4:-60964143 None:intergenic
ACTATTGGAACACCTCCTGG+TGG 0.679776 5.4:+60965929 MS.gene041990:CDS
GTATGCAACAGAAACATTTG+GGG 0.682681 5.4:-60964755 None:intergenic
ACCTTGTCGATCCACCTGTG+AGG 0.686020 5.4:+60965903 MS.gene041990:CDS
GTCAAATGCAGCATTCATGG+CGG 0.689483 5.4:-60965297 None:intergenic
GGCAAAATAGGTCTTGAAGG+TGG 0.702577 5.4:-60965359 None:intergenic
GCCATGTAGTATCGGCCGGG+TGG 0.704138 5.4:-60965257 None:intergenic
CAATGTCCAATCCAACCAGG+TGG 0.711993 5.4:+60964043 MS.gene041990:CDS
ATGGCGACCCATCCACCAGG+AGG 0.719284 5.4:-60965941 None:intergenic
AGAGGCTGTGCAAAACACGA+AGG 0.719687 5.4:+60963729 MS.gene041990:CDS
AGGAACAGGAGGAAAATCTG+TGG 0.731627 5.4:-60965654 None:intergenic
TATGCAACAGAAACATTTGG+GGG 0.741509 5.4:-60964754 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCATTATTTATCCAAAAAT+GGG + chr5.4:60964160-60964179 MS.gene041990:CDS 15.0%
!! TTTCATTATTTATCCAAAAA+TGG + chr5.4:60964159-60964178 MS.gene041990:CDS 15.0%
!!! AAAAAATGAGTTCTAAATTA+TGG - chr5.4:60964999-60965018 None:intergenic 15.0%
!! AAAAACATATTCTGTCAATA+TGG - chr5.4:60964664-60964683 None:intergenic 20.0%
!! ACTTAAACTCCTTTATATTA+CGG + chr5.4:60964966-60964985 MS.gene041990:intron 20.0%
!! TAACATAAATGAATTATACC+TGG - chr5.4:60965986-60966005 None:intergenic 20.0%
!! TATTAAGAAGTTAAACTAGA+GGG - chr5.4:60964595-60964614 None:intergenic 20.0%
!! TGAATTCAAAAATCAACATT+TGG - chr5.4:60966252-60966271 None:intergenic 20.0%
!! TTATTAAGAAGTTAAACTAG+AGG - chr5.4:60964596-60964615 None:intergenic 20.0%
!!! AATATGTTTTTATGATGCAT+AGG + chr5.4:60964673-60964692 MS.gene041990:intron 20.0%
!!! ATATGTTTTTATGATGCATA+GGG + chr5.4:60964674-60964693 MS.gene041990:intron 20.0%
!!! ATGTGAATGTTTTTAAATCA+TGG + chr5.4:60966160-60966179 MS.gene041990:intron 20.0%
!!! ATTTCGAAACACTTTAATTT+TGG - chr5.4:60965450-60965469 None:intergenic 20.0%
!!! TAAATACCAAGTTTGAAATA+TGG + chr5.4:60964426-60964445 MS.gene041990:intron 20.0%
! AAAACGATGTATGAAAGTAA+TGG - chr5.4:60964848-60964867 None:intergenic 25.0%
! ATAGTGAATGTTTGGATAAA+CGG - chr5.4:60963931-60963950 None:intergenic 25.0%
! ATATTTCAACTTGAACAACT+TGG - chr5.4:60965741-60965760 None:intergenic 25.0%
! ATGAATTATACCTGGATTAT+CGG - chr5.4:60965978-60965997 None:intergenic 25.0%
! ATTAAGAAGTTAAACTAGAG+GGG - chr5.4:60964594-60964613 None:intergenic 25.0%
! GTATAAAACAATTACAATGC+AGG + chr5.4:60965029-60965048 MS.gene041990:intron 25.0%
! TAACTAACTTTAATGATTGC+TGG + chr5.4:60963681-60963700 MS.gene041990:intron 25.0%
! TACTAGTATAGTAACAATTG+AGG + chr5.4:60965790-60965809 MS.gene041990:CDS 25.0%
! TAGTTAATACAGTTGCAAAT+TGG - chr5.4:60963667-60963686 None:intergenic 25.0%
! TCTCTTGCATAATTTATACT+AGG + chr5.4:60966202-60966221 MS.gene041990:intron 25.0%
! TTTACAATTCAAAACCAAGA+AGG + chr5.4:60964082-60964101 MS.gene041990:CDS 25.0%
! TTTGATAAACTGTTCGAAAT+AGG + chr5.4:60964280-60964299 MS.gene041990:intron 25.0%
!! AAGTATTTAAGACCATAATG+AGG - chr5.4:60963886-60963905 None:intergenic 25.0%
!!! AACTCATTTTTTCGTACTAA+AGG + chr5.4:60965007-60965026 MS.gene041990:intron 25.0%
!!! AAGTTGTTCAAGTTGAAATA+TGG + chr5.4:60965740-60965759 MS.gene041990:CDS 25.0%
!!! ACTATTTTATGCTACTTACT+TGG + chr5.4:60963539-60963558 MS.gene041990:CDS 25.0%
!!! CTTGGTTTTGAATTGTAAAT+CGG - chr5.4:60964081-60964100 None:intergenic 25.0%
!!! TATGTTTTTATGATGCATAG+GGG + chr5.4:60964675-60964694 MS.gene041990:intron 25.0%
!!! TTTACCATTTTTTAACGACA+CGG + chr5.4:60965385-60965404 MS.gene041990:CDS 25.0%
AAACACTTACAACAAACTGA+TGG - chr5.4:60963610-60963629 None:intergenic 30.0%
AATGGTCCATATTTCAAACT+TGG - chr5.4:60964435-60964454 None:intergenic 30.0%
ACATAGATTCACATCTCAAT+TGG + chr5.4:60966307-60966326 MS.gene041990:CDS 30.0%
ATAGATTCACATCTCAATTG+GGG + chr5.4:60966309-60966328 MS.gene041990:CDS 30.0%
ATTATGCAAGAGAGAGATAT+CGG - chr5.4:60966195-60966214 None:intergenic 30.0%
ATTGTAGTAAGCTTGCATTA+AGG - chr5.4:60965615-60965634 None:intergenic 30.0%
ATTGTTGTGAATCAGGAATT+TGG + chr5.4:60966271-60966290 MS.gene041990:intron 30.0%
CACATTTAGCCGTAATATAA+AGG - chr5.4:60964904-60964923 None:intergenic 30.0%
CATAGATTCACATCTCAATT+GGG + chr5.4:60966308-60966327 MS.gene041990:CDS 30.0%
CTGAACCAACTATAAAGAAA+TGG - chr5.4:60965841-60965860 None:intergenic 30.0%
CTGCAAAACAATTTGAACAT+TGG - chr5.4:60965768-60965787 None:intergenic 30.0%
CTTTATATTACGGCTAAATG+TGG + chr5.4:60964902-60964921 MS.gene041990:intron 30.0%
GACCATTCAATCTTTATCAA+TGG + chr5.4:60964448-60964467 MS.gene041990:intron 30.0%
TAAAACACTGACACAAACAT+CGG - chr5.4:60966134-60966153 None:intergenic 30.0%
TAGTGAATGTTTGGATAAAC+GGG - chr5.4:60963930-60963949 None:intergenic 30.0%
TCCAAATCCAAATCGAAATT+CGG - chr5.4:60964387-60964406 None:intergenic 30.0%
TCGTTAAAAAATGGTAAAGC+CGG - chr5.4:60965383-60965402 None:intergenic 30.0%
TGAATTCATTGTTGTGAATC+AGG + chr5.4:60966264-60966283 MS.gene041990:intron 30.0%
TGAGTTTATTGTTCACTGTA+GGG + chr5.4:60965477-60965496 MS.gene041990:CDS 30.0%
TGTATGCAACAGAAACATTT+GGG - chr5.4:60964759-60964778 None:intergenic 30.0%
TTAATGCAAGCTTACTACAA+TGG + chr5.4:60965614-60965633 MS.gene041990:CDS 30.0%
TTGAAGATTACCAAGAAGAA+CGG - chr5.4:60964224-60964243 None:intergenic 30.0%
! CATTTCTTTATAGTTGGTTC+AGG + chr5.4:60965839-60965858 MS.gene041990:CDS 30.0%
! TCTTAAGAGAATTGTTTCTC+CGG - chr5.4:60965078-60965097 None:intergenic 30.0%
! TGTTTGTGTCAGTGTTTTAT+AGG + chr5.4:60966135-60966154 MS.gene041990:intron 30.0%
! TTTATCAATGGCCATTGATT+TGG + chr5.4:60964460-60964479 MS.gene041990:intron 30.0%
!! ATGTTTTTAAATCATGGTCG+CGG + chr5.4:60966166-60966185 MS.gene041990:intron 30.0%
!! CAATGTTCAAATTGTTTTGC+AGG + chr5.4:60965766-60965785 MS.gene041990:CDS 30.0%
!! TTTATAGTTGGTTCAGGTTT+TGG + chr5.4:60965845-60965864 MS.gene041990:CDS 30.0%
AAAACCAAGAAGGAACACTA+TGG + chr5.4:60964092-60964111 MS.gene041990:CDS 35.0%
AAATCTGTGGTAAAAACACC+AGG - chr5.4:60965644-60965663 None:intergenic 35.0%
AGACACATAAGAAGATTGTC+AGG - chr5.4:60963957-60963976 None:intergenic 35.0%
ATGGTTTGATAGAGATCCAA+TGG + chr5.4:60964699-60964718 MS.gene041990:CDS 35.0%
ATTACACAGGAAATGTTCCT+AGG + chr5.4:60965690-60965709 MS.gene041990:CDS 35.0%
CCTATACAGTTCAATTACAC+AGG + chr5.4:60965677-60965696 MS.gene041990:CDS 35.0%
CCTGTGTAATTGAACTGTAT+AGG - chr5.4:60965680-60965699 None:intergenic 35.0%
CGCTGCAAACCTTTATATTA+CGG + chr5.4:60964892-60964911 MS.gene041990:intron 35.0%
GAATGATTTCAAACCTTGAC+TGG - chr5.4:60964818-60964837 None:intergenic 35.0%
GGCCATTGATAAAGATTGAA+TGG - chr5.4:60964453-60964472 None:intergenic 35.0%
GGGTTCCATTTCTTTATAGT+TGG + chr5.4:60965833-60965852 MS.gene041990:CDS 35.0%
GTATGCAACAGAAACATTTG+GGG - chr5.4:60964758-60964777 None:intergenic 35.0%
GTGAGTTTATTGTTCACTGT+AGG + chr5.4:60965476-60965495 MS.gene041990:CDS 35.0%
GTGTAATTGAACTGTATAGG+AGG - chr5.4:60965677-60965696 None:intergenic 35.0%
GTGTATGCAACAGAAACATT+TGG - chr5.4:60964760-60964779 None:intergenic 35.0%
TAGGATGTTGAGAAATCCAT+GGG + chr5.4:60963997-60964016 MS.gene041990:CDS 35.0%
TATGCAACAGAAACATTTGG+GGG - chr5.4:60964757-60964776 None:intergenic 35.0%
TCAGGAACATAGTGAATGTT+TGG - chr5.4:60963939-60963958 None:intergenic 35.0%
TGAAGATTACCAAGAAGAAC+GGG - chr5.4:60964223-60964242 None:intergenic 35.0%
TGCATTAAGGAAGTTGTTCT+AGG - chr5.4:60965602-60965621 None:intergenic 35.0%
TTAAGACAAACACAGTTCAC+CGG + chr5.4:60964727-60964746 MS.gene041990:CDS 35.0%
TTACACAGGAAATGTTCCTA+GGG + chr5.4:60965691-60965710 MS.gene041990:CDS 35.0%
TTAGGATGTTGAGAAATCCA+TGG + chr5.4:60963996-60964015 MS.gene041990:CDS 35.0%
TTATTGTTCACTGTAGGGTT+AGG + chr5.4:60965482-60965501 MS.gene041990:CDS 35.0%
! AACAACTTGGTTCCTTTTCT+AGG - chr5.4:60965728-60965747 None:intergenic 35.0%
! ACAGCACTTTTGGTAGAAAA+TGG + chr5.4:60966339-60966358 MS.gene041990:CDS 35.0%
! CTTTTGGTAGAAAATGGAGT+TGG + chr5.4:60966345-60966364 MS.gene041990:CDS 35.0%
! TTTTATGATGCATAGGGGAA+TGG + chr5.4:60964680-60964699 MS.gene041990:intron 35.0%
!! ACATCGTTTTAAATTGCAGC+TGG + chr5.4:60964858-60964877 MS.gene041990:intron 35.0%
!! TCCGAATTTCGATTTGGATT+TGG + chr5.4:60964383-60964402 MS.gene041990:intron 35.0%
AACACTAGAGTCCTCATGAT+AGG + chr5.4:60965194-60965213 MS.gene041990:CDS 40.0%
AATACTATTGGAACACCTCC+TGG + chr5.4:60965926-60965945 MS.gene041990:CDS 40.0%
AATCTTGATCCAATCGACCA+TGG - chr5.4:60964357-60964376 None:intergenic 40.0%
AATGAAAGCTCCATAGACAG+TGG - chr5.4:60964146-60964165 None:intergenic 40.0%
AATGGTAAAGCCGGCAAAAT+AGG - chr5.4:60965374-60965393 None:intergenic 40.0%
AGAACGGGAAATTCTCTAGT+TGG - chr5.4:60964208-60964227 None:intergenic 40.0%
AGAGAATTTCCCGTTCTTCT+TGG + chr5.4:60964211-60964230 MS.gene041990:CDS 40.0%
ATCACCCTATGCATGTTCAT+GGG + chr5.4:60965813-60965832 MS.gene041990:CDS 40.0%
ATGGTCGATTGGATCAAGAT+TGG + chr5.4:60964356-60964375 MS.gene041990:intron 40.0%
ATTATCGGCCTTGAAACGAA+TGG - chr5.4:60965963-60965982 None:intergenic 40.0%
CAGAATGACTGTTGTTGCAA+CGG + chr5.4:60965148-60965167 MS.gene041990:CDS 40.0%
CTTATGTGTCTTTGTGCAAC+AGG + chr5.4:60963966-60963985 MS.gene041990:intron 40.0%
GCACTAATCCTAATAGTCCA+AGG + chr5.4:60965516-60965535 MS.gene041990:CDS 40.0%
GCATTCACATCAAGGATTAG+AGG + chr5.4:60965419-60965438 MS.gene041990:CDS 40.0%
GCTGCAAATCTTGTTCCATT+TGG - chr5.4:60965551-60965570 None:intergenic 40.0%
GGACATTGTGTCACATAACT+AGG - chr5.4:60964031-60964050 None:intergenic 40.0%
GGCATAGACTTGAAAACGTA+CGG - chr5.4:60965057-60965076 None:intergenic 40.0%
GGTCTGCAACCGTAATATAA+AGG - chr5.4:60964978-60964997 None:intergenic 40.0%
GTGATCCAAGTAACAAATGC+TGG + chr5.4:60963815-60963834 MS.gene041990:CDS 40.0%
GTTCCAATAGTATTCCTCAC+AGG - chr5.4:60965920-60965939 None:intergenic 40.0%
GTTGTCAAATGCAGCATTCA+TGG - chr5.4:60965303-60965322 None:intergenic 40.0%
GTTTGTCTTAAGAGAGCCAT+TGG - chr5.4:60964718-60964737 None:intergenic 40.0%
TACACAGGAAATGTTCCTAG+GGG + chr5.4:60965692-60965711 MS.gene041990:CDS 40.0%
TATCATGAGGACTCTAGTGT+TGG - chr5.4:60965195-60965214 None:intergenic 40.0%
TCGATTGGATCAAGATTGGA+CGG + chr5.4:60964360-60964379 MS.gene041990:intron 40.0%
TTCAAACTGCAACAGTGAAG+AGG + chr5.4:60963711-60963730 MS.gene041990:CDS 40.0%
TTCACATCTCAATTGGGGTT+TGG + chr5.4:60966314-60966333 MS.gene041990:CDS 40.0%
TTCGCAGTAACAATCTCATG+CGG + chr5.4:60964313-60964332 MS.gene041990:intron 40.0%
TTGAACTGTATAGGAGGAAC+AGG - chr5.4:60965671-60965690 None:intergenic 40.0%
TTGCAACAACAGTCATTCTG+TGG - chr5.4:60965148-60965167 None:intergenic 40.0%
! AAGGTCCAGCATTTGTTACT+TGG - chr5.4:60963823-60963842 None:intergenic 40.0%
! ACCTTCAAGACCTATTTTGC+CGG + chr5.4:60965361-60965380 MS.gene041990:CDS 40.0%
! AGGACATTGATTGTTTGACC+TGG - chr5.4:60965221-60965240 None:intergenic 40.0%
! GAGGTATCACTGACTGTAAT+GGG - chr5.4:60966371-60966390 None:intergenic 40.0%
! GGATAAGCAGAACCCATTTT+TGG - chr5.4:60964175-60964194 None:intergenic 40.0%
! GTCTTGAAGGTGGTCTATTT+TGG - chr5.4:60965352-60965371 None:intergenic 40.0%
! TCTCTAGTTGGCTTAGAGAA+AGG - chr5.4:60964196-60964215 None:intergenic 40.0%
!!! GTACGTTTTCAAGTCTATGC+CGG + chr5.4:60965056-60965075 MS.gene041990:CDS 40.0%
AACGTAGCAGGATCAGTTCT+TGG - chr5.4:60965881-60965900 None:intergenic 45.0%
AACTGTATAGGAGGAACAGG+AGG - chr5.4:60965668-60965687 None:intergenic 45.0%
ACACAATGTCCAATCCAACC+AGG + chr5.4:60964040-60964059 MS.gene041990:CDS 45.0%
ACCAAGAAGGAACACTATGG+TGG + chr5.4:60964095-60964114 MS.gene041990:CDS 45.0%
ACCGTAAATGGACAGTTTCC+TGG + chr5.4:60963758-60963777 MS.gene041990:CDS 45.0%
AGGAACAGGAGGAAAATCTG+TGG - chr5.4:60965657-60965676 None:intergenic 45.0%
ATCGTCACTCGTCCTCATTA+TGG + chr5.4:60963871-60963890 MS.gene041990:intron 45.0%
ATCTCCTGGTTGACCATTCA+CGG - chr5.4:60964782-60964801 None:intergenic 45.0%
ATGTGTAACTTCCACCTGGT+TGG - chr5.4:60964057-60964076 None:intergenic 45.0%
CACACAGTGAGCCAAATCAA+TGG - chr5.4:60964474-60964493 None:intergenic 45.0%
CACATATGCCAGTGTCGTAT+CGG - chr5.4:60966046-60966065 None:intergenic 45.0%
CAGTGGATGGAGATGTTGTA+AGG - chr5.4:60963842-60963861 None:intergenic 45.0%
CCAATAGTATTCCTCACAGG+TGG - chr5.4:60965917-60965936 None:intergenic 45.0%
CCACCTGTGAGGAATACTAT+TGG + chr5.4:60965914-60965933 MS.gene041990:CDS 45.0%
CCTTGACACCTTGGACTATT+AGG - chr5.4:60965527-60965546 None:intergenic 45.0%
CGAAGGATACTCACCGTAAA+TGG + chr5.4:60963746-60963765 MS.gene041990:CDS 45.0%
CGATGATAGTTACCAGTGGA+TGG - chr5.4:60963855-60963874 None:intergenic 45.0%
CTATAGAACCGATACGACAC+TGG + chr5.4:60966035-60966054 MS.gene041990:intron 45.0%
CTCTATCGATGTTCCAGTCA+AGG + chr5.4:60964802-60964821 MS.gene041990:CDS 45.0%
CTGCAACTGCATTCACATCA+AGG + chr5.4:60965411-60965430 MS.gene041990:CDS 45.0%
CTTACAACATCTCCATCCAC+TGG + chr5.4:60963840-60963859 MS.gene041990:CDS 45.0%
GAAATGTTCCTAGGGGTTTG+TGG + chr5.4:60965699-60965718 MS.gene041990:CDS 45.0%
GAACATCGATAGAGATCTCC+TGG - chr5.4:60964796-60964815 None:intergenic 45.0%
GATCACCCTATGCATGTTCA+TGG + chr5.4:60965812-60965831 MS.gene041990:CDS 45.0%
GCCAGGAAACTGTCCATTTA+CGG - chr5.4:60963762-60963781 None:intergenic 45.0%
GCTTACTACAATGGTGTTCC+TGG + chr5.4:60965623-60965642 MS.gene041990:CDS 45.0%
GGAACCCATGAACATGCATA+GGG - chr5.4:60965820-60965839 None:intergenic 45.0%
GGCAAAATAGGTCTTGAAGG+TGG - chr5.4:60965362-60965381 None:intergenic 45.0%
GGTTCTATAGCTAGAGCACA+TGG - chr5.4:60966025-60966044 None:intergenic 45.0%
GGTTTGTGGACACCTAGAAA+AGG + chr5.4:60965713-60965732 MS.gene041990:CDS 45.0%
GTCAAATGCAGCATTCATGG+CGG - chr5.4:60965300-60965319 None:intergenic 45.0%
GTGACGATGATAGTTACCAG+TGG - chr5.4:60963859-60963878 None:intergenic 45.0%
TAACTTCCACCTGGTTGGAT+TGG - chr5.4:60964052-60964071 None:intergenic 45.0%
TCTGTTGCATACACCGTGAA+TGG + chr5.4:60964766-60964785 MS.gene041990:CDS 45.0%
TGATGTGAATGCAGTTGCAG+TGG - chr5.4:60965411-60965430 None:intergenic 45.0%
TGGAACCCATGAACATGCAT+AGG - chr5.4:60965821-60965840 None:intergenic 45.0%
TGTCTTTGTGCAACAGGCAT+GGG + chr5.4:60963972-60963991 MS.gene041990:intron 45.0%
TTCCTAAGAGCCACTGTCTA+TGG + chr5.4:60964133-60964152 MS.gene041990:CDS 45.0%
TTGAGAAATCCATGGGCAGA+TGG + chr5.4:60964004-60964023 MS.gene041990:CDS 45.0%
TTTGACCTGGTCCTATCATG+AGG - chr5.4:60965208-60965227 None:intergenic 45.0%
! ATGAGGACTCTAGTGTTGGA+AGG - chr5.4:60965191-60965210 None:intergenic 45.0%
! CCTAATAGTCCAAGGTGTCA+AGG + chr5.4:60965524-60965543 MS.gene041990:CDS 45.0%
! GTGGCCGTGTCGTTAAAAAA+TGG - chr5.4:60965392-60965411 None:intergenic 45.0%
! TAGCATTGAGGTAGATCTGG+TGG - chr5.4:60966396-60966415 None:intergenic 45.0%
! TGTTTCATTTGCTGCAGCAG+AGG + chr5.4:60963577-60963596 MS.gene041990:CDS 45.0%
!! GGAGGTATCACTGACTGTAA+TGG - chr5.4:60966372-60966391 None:intergenic 45.0%
ACTATTGGAACACCTCCTGG+TGG + chr5.4:60965929-60965948 MS.gene041990:CDS 50.0%
AGAGGCTGTGCAAAACACGA+AGG + chr5.4:60963729-60963748 MS.gene041990:CDS 50.0%
AGAGTCCTCATGATAGGACC+AGG + chr5.4:60965200-60965219 MS.gene041990:CDS 50.0%
ATGCAGCATTCATGGCGGTT+TGG - chr5.4:60965295-60965314 None:intergenic 50.0%
ATGGGTCGCCATTCGTTTCA+AGG + chr5.4:60965952-60965971 MS.gene041990:CDS 50.0%
CAATGTCCAATCCAACCAGG+TGG + chr5.4:60964043-60964062 MS.gene041990:CDS 50.0%
CACACAGTTAGCCACATCCA+TGG + chr5.4:60964337-60964356 MS.gene041990:intron 50.0%
CATGGATGTGGCTAACTGTG+TGG - chr5.4:60964339-60964358 None:intergenic 50.0%
CATTGAGGTAGATCTGGTGG+TGG - chr5.4:60966393-60966412 None:intergenic 50.0%
CCATTTGGTCCTTGACACCT+TGG - chr5.4:60965536-60965555 None:intergenic 50.0%
CGGTATGTGTAACTTCCACC+TGG - chr5.4:60964061-60964080 None:intergenic 50.0%
CGTTTCAAGGCCGATAATCC+AGG + chr5.4:60965965-60965984 MS.gene041990:CDS 50.0%
CTCCATAGACAGTGGCTCTT+AGG - chr5.4:60964138-60964157 None:intergenic 50.0%
GATCCAATCGACCATGGATG+TGG - chr5.4:60964351-60964370 None:intergenic 50.0%
GCCACCATAGTGTTCCTTCT+TGG - chr5.4:60964099-60964118 None:intergenic 50.0%
GCCGGCAAAATAGGTCTTGA+AGG - chr5.4:60965365-60965384 None:intergenic 50.0%
GGACGGTCCGAATTTCGATT+TGG + chr5.4:60964377-60964396 MS.gene041990:intron 50.0%
GTGTCTTTGTGCAACAGGCA+TGG + chr5.4:60963971-60963990 MS.gene041990:intron 50.0%
TAACTAGGTCCATCTGCCCA+TGG - chr5.4:60964016-60964035 None:intergenic 50.0%
TACACCGTGAATGGTCAACC+AGG + chr5.4:60964775-60964794 MS.gene041990:CDS 50.0%
TAGCCACATCCATGGTCGAT+TGG + chr5.4:60964345-60964364 MS.gene041990:intron 50.0%
TCGACAAGGTTGAACGTAGC+AGG - chr5.4:60965893-60965912 None:intergenic 50.0%
! AGCGAGGGACGGAGATTTTA+TGG + chr5.4:60963793-60963812 MS.gene041990:CDS 50.0%
! CCAAGGTGTCAAGGACCAAA+TGG + chr5.4:60965533-60965552 MS.gene041990:CDS 50.0%
! GGGTTTGGCAACAGCACTTT+TGG + chr5.4:60966329-60966348 MS.gene041990:CDS 50.0%
!! AGAAACATTTGGGGGTGCTC+CGG - chr5.4:60964749-60964768 None:intergenic 50.0%
ACCTTGTCGATCCACCTGTG+AGG + chr5.4:60965903-60965922 MS.gene041990:CDS 55.0%
CGCGTGCTGCCATGTAGTAT+CGG - chr5.4:60965268-60965287 None:intergenic 55.0%
CTAGGTGTCCACAAACCCCT+AGG - chr5.4:60965710-60965729 None:intergenic 55.0%
CTATGGTGGCATGCTCACAC+TGG + chr5.4:60964109-60964128 MS.gene041990:CDS 55.0%
GTGGTTGATCGGCTGTGACA+AGG - chr5.4:60965241-60965260 None:intergenic 55.0%
TCCTCACAGGTGGATCGACA+AGG - chr5.4:60965907-60965926 None:intergenic 55.0%
TGTGCAACAGGCATGGGCTT+AGG + chr5.4:60963978-60963997 MS.gene041990:intron 55.0%
TTGGAACACCTCCTGGTGGA+TGG + chr5.4:60965933-60965952 MS.gene041990:CDS 55.0%
! TGGAACACCTCCTGGTGGAT+GGG + chr5.4:60965934-60965953 MS.gene041990:CDS 55.0%
!! TGAGGTAGATCTGGTGGTGG+AGG - chr5.4:60966390-60966409 None:intergenic 55.0%
!! TGCTGCCATGTAGTATCGGC+CGG - chr5.4:60965264-60965283 None:intergenic 55.0%
ACCACCCGGCCGATACTACA+TGG + chr5.4:60965256-60965275 MS.gene041990:CDS 60.0%
CCAACGATCGAAGCGAGGGA+CGG + chr5.4:60963782-60963801 MS.gene041990:CDS 60.0%
CGTCCCTCGCTTCGATCGTT+GGG - chr5.4:60963784-60963803 None:intergenic 60.0%
GTCACAGCCGATCAACCACC+CGG + chr5.4:60965242-60965261 MS.gene041990:CDS 60.0%
TGGCCCAACGATCGAAGCGA+GGG + chr5.4:60963778-60963797 MS.gene041990:CDS 60.0%
! GTATCGGCCGGGTGGTTGAT+CGG - chr5.4:60965252-60965271 None:intergenic 60.0%
!! GCTGCCATGTAGTATCGGCC+GGG - chr5.4:60965263-60965282 None:intergenic 60.0%
ATGGCGACCCATCCACCAGG+AGG - chr5.4:60965944-60965963 None:intergenic 65.0%
CCGTCCCTCGCTTCGATCGT+TGG - chr5.4:60963785-60963804 None:intergenic 65.0%
CGAATGGCGACCCATCCACC+AGG - chr5.4:60965947-60965966 None:intergenic 65.0%
CTCGCTTCGATCGTTGGGCC+AGG - chr5.4:60963779-60963798 None:intergenic 65.0%
CTGGCCCAACGATCGAAGCG+AGG + chr5.4:60963777-60963796 MS.gene041990:CDS 65.0%
!! GCCATGTAGTATCGGCCGGG+TGG - chr5.4:60965260-60965279 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 60963533 60966416 60963533 ID=MS.gene041990
chr5.4 mRNA 60963533 60966416 60963533 ID=MS.gene041990.t1;Parent=MS.gene041990
chr5.4 exon 60963533 60963619 60963533 ID=MS.gene041990.t1.exon1;Parent=MS.gene041990.t1
chr5.4 CDS 60963533 60963619 60963533 ID=cds.MS.gene041990.t1;Parent=MS.gene041990.t1
chr5.4 exon 60963710 60963861 60963710 ID=MS.gene041990.t1.exon2;Parent=MS.gene041990.t1
chr5.4 CDS 60963710 60963861 60963710 ID=cds.MS.gene041990.t1;Parent=MS.gene041990.t1
chr5.4 exon 60963988 60964232 60963988 ID=MS.gene041990.t1.exon3;Parent=MS.gene041990.t1
chr5.4 CDS 60963988 60964232 60963988 ID=cds.MS.gene041990.t1;Parent=MS.gene041990.t1
chr5.4 exon 60964695 60964823 60964695 ID=MS.gene041990.t1.exon4;Parent=MS.gene041990.t1
chr5.4 CDS 60964695 60964823 60964695 ID=cds.MS.gene041990.t1;Parent=MS.gene041990.t1
chr5.4 exon 60965051 60965986 60965051 ID=MS.gene041990.t1.exon5;Parent=MS.gene041990.t1
chr5.4 CDS 60965051 60965986 60965051 ID=cds.MS.gene041990.t1;Parent=MS.gene041990.t1
chr5.4 exon 60966286 60966416 60966286 ID=MS.gene041990.t1.exon6;Parent=MS.gene041990.t1
chr5.4 CDS 60966286 60966416 60966286 ID=cds.MS.gene041990.t1;Parent=MS.gene041990.t1
Gene Sequence

>MS.gene041990

ATGAAGACTATTTTATGCTACTTACTTGGCTTACTTACTATCATTGTTTCATTTGCTGCAGCAGAGGAGCATTACCATCAGTTTGTTGTAAGTGTTTGTTTGATGATCACATTCATATATATATGTTTGTACCAATTTGCAACTGTATTAACTAACTTTAATGATTGCTGGAAATAGATTCAAACTGCAACAGTGAAGAGGCTGTGCAAAACACGAAGGATACTCACCGTAAATGGACAGTTTCCTGGCCCAACGATCGAAGCGAGGGACGGAGATTTTATGGTGATCCAAGTAACAAATGCTGGACCTTACAACATCTCCATCCACTGGTAACTATCATCGTCACTCGTCCTCATTATGGTCTTAAATACTTGTATGATAAGCGCTTAATTAACCCGTTTATCCAAACATTCACTATGTTCCTGACAATCTTCTTATGTGTCTTTGTGCAACAGGCATGGGCTTAGGATGTTGAGAAATCCATGGGCAGATGGACCTAGTTATGTGACACAATGTCCAATCCAACCAGGTGGAAGTTACACATACCGATTTACAATTCAAAACCAAGAAGGAACACTATGGTGGCATGCTCACACTGGTTTCCTAAGAGCCACTGTCTATGGAGCTTTCATTATTTATCCAAAAATGGGTTCTGCTTATCCTTTCTCTAAGCCAACTAGAGAATTTCCCGTTCTTCTTGGTAATCTTCAAAACTATCATCACTTTATTATACTTAAAGAGAGCTAGTTTGATAAACTGTTCGAAATAGGATTTGAAATGTTCGCAGTAACAATCTCATGCGGCCACACAGTTAGCCACATCCATGGTCGATTGGATCAAGATTGGACGGTCCGAATTTCGATTTGGATTTGGATTTTTTTTTTTTCAGAAACTAAATACCAAGTTTGAAATATGGACCATTCAATCTTTATCAATGGCCATTGATTTGGCTCACTGTGTGATCATATAGCATTGTGACTGCTATATGATTATGAACTTTTCTAACAATAGCTTTTTGTATAAGTAAAAATTAGCTTTTGCATATTAACTTTTTAGAACCCCTCTAGTTTAACTTCTTAATAAACACTTCAAAAGTTGCTCTTAAATTTTTATGCAACAAAGACAGAGCCATATTGACAGAATATGTTTTTATGATGCATAGGGGAATGGTTTGATAGAGATCCAATGGCTCTCTTAAGACAAACACAGTTCACCGGAGCACCCCCAAATGTTTCTGTTGCATACACCGTGAATGGTCAACCAGGAGATCTCTATCGATGTTCCAGTCAAGGTTTGAAATCATTCTAATTCACCATTACTTTCATACATCGTTTTAAATTGCAGCTGGAGTCGCAGTTACGCTGCAAACCTTTATATTACGGCTAAATGTGGATGATGCAATCGAAACTATGATTGTGATACTGTTGCAAAAAACTTAAACTCCTTTATATTACGGTTGCAGACCATAATTTAGAACTCATTTTTTCGTACTAAAGGTATAAAACAATTACAATGCAGGAACCGTACGTTTTCAAGTCTATGCCGGAGAAACAATTCTCTTAAGAATCATCAACAGTGCACTCAATCAAGAACTCTTCTTTTCAATTGCTAACCACAGAATGACTGTTGTTGCAACGGATGCTGCTTACACAAAACCTTCCAACACTAGAGTCCTCATGATAGGACCAGGTCAAACAATCAATGTCCTTGTCACAGCCGATCAACCACCCGGCCGATACTACATGGCAGCACGCGCTTACCAAACCGCCATGAATGCTGCATTTGACAACACAACGACAACTGCAATTCTTGAATACCAAAATAGACCACCTTCAAGACCTATTTTGCCGGCTTTACCATTTTTTAACGACACGGCCACTGCAACTGCATTCACATCAAGGATTAGAGGACTTTCCAAAATTAAAGTGTTTCGAAATGTTGATGTGAGTTTATTGTTCACTGTAGGGTTAGGATTAATAAACTGCACTAATCCTAATAGTCCAAGGTGTCAAGGACCAAATGGAACAAGATTTGCAGCTAGTATAAACAACAACTCTTTTGTTCTTCCTAGAACAACTTCCTTAATGCAAGCTTACTACAATGGTGTTCCTGGTGTTTTTACCACAGATTTTCCTCCTGTTCCTCCTATACAGTTCAATTACACAGGAAATGTTCCTAGGGGTTTGTGGACACCTAGAAAAGGAACCAAGTTGTTCAAGTTGAAATATGGTTCCAATGTTCAAATTGTTTTGCAGGATACTAGTATAGTAACAATTGAGGATCACCCTATGCATGTTCATGGGTTCCATTTCTTTATAGTTGGTTCAGGTTTTGGAAATTTTAATCCAAGAACTGATCCTGCTACGTTCAACCTTGTCGATCCACCTGTGAGGAATACTATTGGAACACCTCCTGGTGGATGGGTCGCCATTCGTTTCAAGGCCGATAATCCAGGTATAATTCATTTATGTTACTCATATACAAAGATGCCATGTGCTCTAGCTATAGAACCGATACGACACTGGCATATGTGATAACGTTCAATTAATTCATATTTTTTTTTAAATTATTACTGATGTCAACGTATCAGTATCATGTCCGATGTTTGTGTCAGTGTTTTATAGGTCATGTGAATGTTTTTAAATCATGGTCGCGGTGTCCGATATCTCTCTCTTGCATAATTTATACTAGGCTCTTAGCATTGTATGTATACTAACCAAATGTTGATTTTTGAATTCATTGTTGTGAATCAGGAATTTGGTTCTTGCATTGTCACATAGATTCACATCTCAATTGGGGTTTGGCAACAGCACTTTTGGTAGAAAATGGAGTTGGCCCATTACAGTCAGTGATACCTCCACCACCAGATCTACCTCAATGCTAA
Protein sequence

>MS.gene041990.t1

MKTILCYLLGLLTIIVSFAAAEEHYHQFVIQTATVKRLCKTRRILTVNGQFPGPTIEARDGDFMVIQVTNAGPYNISIHWHGLRMLRNPWADGPSYVTQCPIQPGGSYTYRFTIQNQEGTLWWHAHTGFLRATVYGAFIIYPKMGSAYPFSKPTREFPVLLGEWFDRDPMALLRQTQFTGAPPNVSVAYTVNGQPGDLYRCSSQGTVRFQVYAGETILLRIINSALNQELFFSIANHRMTVVATDAAYTKPSNTRVLMIGPGQTINVLVTADQPPGRYYMAARAYQTAMNAAFDNTTTTAILEYQNRPPSRPILPALPFFNDTATATAFTSRIRGLSKIKVFRNVDVSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVLPRTTSLMQAYYNGVPGVFTTDFPPVPPIQFNYTGNVPRGLWTPRKGTKLFKLKYGSNVQIVLQDTSIVTIEDHPMHVHGFHFFIVGSGFGNFNPRTDPATFNLVDPPVRNTIGTPPGGWVAIRFKADNPGIWFLHCHIDSHLNWGLATALLVENGVGPLQSVIPPPPDLPQC