AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84742

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84742.t1 MTR_8g027375 97.263 548 15 0 1 548 24 571 0.0 1115
MS.gene84742.t1 MTR_3g462760 48.162 544 263 5 11 548 26 556 0.0 543
MS.gene84742.t1 MTR_5g069680 48.346 544 263 6 11 548 27 558 0.0 538
MS.gene84742.t1 MTR_4g015120 47.156 545 270 6 11 548 24 557 0.0 526
MS.gene84742.t1 MTR_7g062310 45.213 564 284 8 2 548 38 593 2.23e-180 521
MS.gene84742.t1 MTR_5g081810 46.667 540 265 8 17 548 5 529 3.04e-178 513
MS.gene84742.t1 MTR_7g062250 47.027 555 269 10 10 548 33 578 3.52e-177 512
MS.gene84742.t1 MTR_4g064530 47.985 546 260 10 1 532 34 569 1.08e-176 511
MS.gene84742.t1 MTR_7g058690 46.320 557 273 9 10 548 32 580 1.29e-176 511
MS.gene84742.t1 MTR_5g083360 46.000 550 275 5 13 548 26 567 2.50e-176 509
MS.gene84742.t1 MTR_7g060460 46.346 561 278 9 5 548 27 581 4.30e-176 509
MS.gene84742.t1 MTR_5g073210 44.526 548 294 6 5 548 19 560 8.63e-175 505
MS.gene84742.t1 MTR_7g458880 45.196 562 282 9 5 548 27 580 1.64e-174 505
MS.gene84742.t1 MTR_5g020620 45.203 542 279 6 13 548 32 561 9.34e-174 503
MS.gene84742.t1 MTR_5g020600 44.751 543 281 7 13 548 36 566 2.50e-172 499
MS.gene84742.t1 MTR_4g064530 46.958 526 255 10 1 512 34 549 5.13e-161 470
MS.gene84742.t1 MTR_2g008330 41.756 558 308 8 1 548 29 579 1.68e-157 462
MS.gene84742.t1 MTR_7g065970 43.693 547 294 7 13 548 27 570 2.13e-157 461
MS.gene84742.t1 MTR_4g019225 41.606 548 305 8 13 548 28 572 8.51e-153 449
MS.gene84742.t1 MTR_3g101640 42.473 558 305 9 1 548 20 571 1.06e-152 449
MS.gene84742.t1 MTR_3g101635 44.030 536 284 10 7 533 23 551 2.26e-152 448
MS.gene84742.t1 MTR_7g065980 41.918 563 302 10 1 548 19 571 1.21e-149 441
MS.gene84742.t1 MTR_3g071890 45.691 499 263 5 10 504 32 526 2.27e-149 441
MS.gene84742.t1 MTR_8g091390 31.103 553 332 14 11 539 34 561 5.94e-68 230
MS.gene84742.t1 MTR_3g078740 30.249 562 334 19 7 526 22 567 2.87e-64 220
MS.gene84742.t1 MTR_3g078700 29.945 541 316 20 5 526 22 518 1.21e-55 196
MS.gene84742.t1 MTR_3g078730 29.511 532 320 17 11 526 25 517 7.82e-55 194
MS.gene84742.t1 MTR_5g081790 53.061 147 62 1 11 150 24 170 2.10e-51 182
MS.gene84742.t1 MTR_5g081790 37.500 280 151 8 235 506 157 420 4.17e-50 178
MS.gene84742.t1 MTR_3g008580 27.660 517 339 14 7 513 25 516 6.41e-47 173
MS.gene84742.t1 MTR_4g101650 28.023 521 338 14 13 523 29 522 6.71e-47 173
MS.gene84742.t1 MTR_1g090707 27.961 515 324 15 25 529 38 515 1.32e-43 164
MS.gene84742.t1 MTR_4g075690 25.187 536 358 13 5 536 24 520 8.14e-40 152
MS.gene84742.t1 MTR_1g026410 25.612 531 321 15 11 521 29 505 1.42e-38 149
MS.gene84742.t1 MTR_4g049390 25.787 508 336 13 5 508 20 490 2.80e-38 148
MS.gene84742.t1 MTR_8g479380 26.007 546 353 15 11 548 29 531 1.22e-37 146
MS.gene84742.t1 MTR_7g051440 26.750 557 351 18 7 544 25 543 5.45e-37 145
MS.gene84742.t1 MTR_3g115050 25.536 513 344 11 11 521 30 506 6.40e-37 144
MS.gene84742.t1 MTR_4g048350 25.591 508 336 15 5 508 20 489 5.81e-35 138
MS.gene84742.t1 MTR_8g073840 37.912 182 74 3 11 191 30 173 1.48e-34 132
MS.gene84742.t1 MTR_4g083210 26.063 541 344 17 1 528 14 511 1.81e-34 137
MS.gene84742.t1 MTR_4g132320 27.593 511 310 17 11 508 31 494 8.51e-34 135
MS.gene84742.t1 MTR_8g479390 25.045 551 352 17 11 548 31 533 1.13e-33 135
MS.gene84742.t1 MTR_4g075690 25.270 463 304 12 5 464 24 447 1.60e-33 133
MS.gene84742.t1 MTR_2g088080 25.349 501 334 10 11 508 30 493 3.79e-33 133
MS.gene84742.t1 MTR_3g083450 24.377 562 354 17 12 543 27 547 4.04e-33 133
MS.gene84742.t1 MTR_3g083430 24.021 562 356 16 12 543 26 546 8.01e-33 132
MS.gene84742.t1 MTR_5g018270 24.478 527 350 15 11 532 31 514 1.07e-32 132
MS.gene84742.t1 MTR_3g083440 24.021 562 356 17 12 543 26 546 3.74e-32 130
MS.gene84742.t1 MTR_3g083400 24.021 562 356 17 12 543 26 546 4.00e-32 130
MS.gene84742.t1 MTR_3g083390 24.021 562 356 17 12 543 26 546 7.74e-32 129
MS.gene84742.t1 MTR_4g083190 24.089 494 332 15 29 514 45 503 4.32e-31 127
MS.gene84742.t1 MTR_3g083410 23.985 542 341 17 32 543 10 510 2.94e-29 121
MS.gene84742.t1 MTR_7g058670 54.118 85 38 1 68 151 13 97 8.31e-28 107
MS.gene84742.t1 MTR_2g088080 25.000 444 293 10 68 508 6 412 9.41e-24 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84742.t1 AT2G46570 66.058 548 186 0 1 548 22 569 0.0 793
MS.gene84742.t1 AT2G46570 66.736 481 160 0 68 548 4 484 0.0 703
MS.gene84742.t1 AT5G01190 48.188 552 266 5 3 548 21 558 0.0 536
MS.gene84742.t1 AT5G05390 47.778 540 275 4 13 548 29 565 0.0 530
MS.gene84742.t1 AT2G38080 47.156 545 268 8 11 548 27 558 0.0 522
MS.gene84742.t1 AT5G01190 48.381 525 255 4 30 548 30 544 7.07e-179 515
MS.gene84742.t1 AT2G40370 44.920 561 289 6 5 548 23 580 1.49e-178 516
MS.gene84742.t1 AT5G03260 46.984 547 270 8 9 548 24 557 4.04e-178 514
MS.gene84742.t1 AT5G58910 46.266 549 273 6 11 548 30 567 5.76e-178 514
MS.gene84742.t1 AT5G58910 46.448 549 271 7 11 548 30 566 1.91e-177 512
MS.gene84742.t1 AT5G60020 45.276 561 278 11 10 548 24 577 3.34e-172 499
MS.gene84742.t1 AT2G30210 43.876 547 290 7 13 548 30 570 4.67e-171 496
MS.gene84742.t1 AT5G07130 44.846 553 281 9 12 548 25 569 3.56e-170 494
MS.gene84742.t1 AT5G58910 45.896 536 261 7 24 548 6 523 5.63e-170 492
MS.gene84742.t1 AT5G60020 45.554 551 271 11 20 548 2 545 2.84e-169 491
MS.gene84742.t1 AT2G29130 46.691 559 276 11 4 548 23 573 9.49e-169 490
MS.gene84742.t1 AT1G18140 44.483 571 278 13 5 548 23 581 3.31e-167 487
MS.gene84742.t1 AT3G09220 43.178 535 276 9 13 531 28 550 1.03e-153 452
MS.gene84742.t1 AT5G09360 43.199 544 289 9 13 548 38 569 1.76e-153 451
MS.gene84742.t1 AT5G60020 44.894 519 257 11 10 506 24 535 3.34e-152 447
MS.gene84742.t1 AT5G01050 41.941 546 296 7 15 548 32 568 1.41e-149 442
MS.gene84742.t1 AT5G48100 43.421 532 281 9 13 532 24 547 6.07e-149 439
MS.gene84742.t1 AT5G07130 45.697 488 241 9 77 548 5 484 1.13e-147 433
MS.gene84742.t1 AT5G07130 45.697 488 241 9 77 548 5 484 1.13e-147 433
MS.gene84742.t1 AT5G01040 40.809 544 303 7 15 548 32 566 3.10e-144 428
MS.gene84742.t1 AT3G09220 42.549 463 242 9 83 531 1 453 1.07e-125 377
MS.gene84742.t1 AT4G39830 31.283 569 318 17 11 539 36 571 3.25e-70 236
MS.gene84742.t1 AT5G21100 31.194 561 314 19 2 527 19 542 2.20e-66 226
MS.gene84742.t1 AT5G21105 31.541 558 322 19 1 526 29 558 1.61e-65 224
MS.gene84742.t1 AT5G21105 30.684 541 315 19 1 509 29 541 1.09e-55 196
MS.gene84742.t1 AT4G39830 30.279 502 278 16 77 539 6 474 1.03e-52 187
MS.gene84742.t1 AT4G39830 30.279 502 278 16 77 539 8 476 1.16e-52 187
MS.gene84742.t1 AT4G25240 28.624 545 346 14 13 544 31 545 1.76e-48 177
MS.gene84742.t1 AT4G25240 28.624 545 346 14 13 544 31 545 1.76e-48 177
MS.gene84742.t1 AT4G39830 29.142 501 272 15 77 539 8 463 6.99e-48 173
MS.gene84742.t1 AT5G48450 28.327 526 322 17 12 523 30 514 1.16e-47 175
MS.gene84742.t1 AT5G51480 27.878 556 346 17 13 548 30 550 2.28e-47 174
MS.gene84742.t1 AT5G48450 28.517 526 321 18 12 523 30 514 2.33e-47 175
MS.gene84742.t1 AT4G12420 27.307 531 343 16 12 529 26 526 2.31e-46 171
MS.gene84742.t1 AT4G12420 27.307 531 343 16 12 529 26 526 2.31e-46 171
MS.gene84742.t1 AT1G75790 26.727 550 333 19 13 543 29 527 7.92e-43 161
MS.gene84742.t1 AT1G21850 27.273 517 328 16 11 521 29 503 4.03e-40 153
MS.gene84742.t1 AT5G66920 24.359 546 372 12 6 548 31 538 7.57e-40 152
MS.gene84742.t1 AT1G21860 25.191 524 330 11 11 521 29 503 1.34e-39 152
MS.gene84742.t1 AT5G21105 32.353 340 198 12 196 526 51 367 1.86e-38 145
MS.gene84742.t1 AT4G28090 24.314 547 362 17 5 544 22 523 1.34e-35 140
MS.gene84742.t1 AT4G22010 25.812 554 346 21 11 548 26 530 1.62e-34 137
MS.gene84742.t1 AT4G38420 24.750 501 343 13 11 508 30 499 7.88e-34 135
MS.gene84742.t1 AT1G76160 23.735 514 351 12 11 521 28 503 2.51e-33 134
MS.gene84742.t1 AT2G23630 25.314 557 341 15 12 548 36 537 1.95e-32 131
MS.gene84742.t1 AT2G23630 25.314 557 341 15 12 548 31 532 2.80e-32 130
MS.gene84742.t1 AT4G37160 25.046 539 366 14 12 548 32 534 4.99e-32 130
MS.gene84742.t1 AT1G41830 23.495 515 351 14 11 521 29 504 6.87e-32 129
MS.gene84742.t1 AT1G55570 25.992 504 299 16 29 508 47 500 1.33e-30 126
MS.gene84742.t1 AT4G38420 24.124 485 334 13 11 492 30 483 8.35e-30 123
MS.gene84742.t1 AT1G55560 24.855 519 329 18 7 508 22 496 1.00e-29 123
MS.gene84742.t1 AT3G13390 25.301 498 324 16 22 508 39 499 2.43e-29 122
MS.gene84742.t1 AT4G38420 24.454 458 309 13 11 464 30 454 9.78e-29 119
MS.gene84742.t1 AT3G13400 23.462 520 339 17 5 508 21 497 4.26e-27 115
MS.gene84742.t1 AT3G13400 23.462 520 339 17 5 508 21 497 4.26e-27 115
MS.gene84742.t1 AT4G37160 27.397 292 195 7 12 302 32 307 3.85e-22 99.8

Find 150 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGGGTACCTTTCCCATTTA+AGG 0.142601 8.4:+69554122 MS.gene84742:CDS
GTGTCATATGGAATGTTATT+AGG 0.191294 8.4:-69555532 None:intergenic
TCTTGATAAGGAGCCTTAAA+TGG 0.196962 8.4:-69554135 None:intergenic
AACATTGAAAGGTGTCTTAT+TGG 0.207388 8.4:-69553809 None:intergenic
AAGTTCTACGACTTCGTTAA+TGG 0.217996 8.4:+69555505 MS.gene84742:CDS
TTTGGCATGCTCATGTTTCT+TGG 0.221846 8.4:+69554055 MS.gene84742:CDS
ATTCACCACTGTAAAGTTAT+AGG 0.222232 8.4:-69554016 None:intergenic
ACTCCAATTGTATTCATATA+TGG 0.236387 8.4:-69555745 None:intergenic
AACTGTCAAGCCATGAATAA+TGG 0.250740 8.4:+69555364 MS.gene84742:CDS
GGGAAAGGTACCCTATTCTT+TGG 0.277356 8.4:-69554114 None:intergenic
GCTTATTATAAGAAAATAAA+TGG 0.283522 8.4:+69555448 MS.gene84742:CDS
ACTAACTGCACAATTCAATT+TGG 0.283999 8.4:+69555714 MS.gene84742:CDS
TTATTTGAGCAGTTGTAGTT+AGG 0.302065 8.4:-69554378 None:intergenic
TGTCATATGGAATGTTATTA+GGG 0.310467 8.4:-69555531 None:intergenic
AACTACAACTGCTCAAATAA+TGG 0.313353 8.4:+69554381 MS.gene84742:CDS
ATCAAAAGGGCACTTTCTTT+TGG 0.315039 8.4:+69554037 MS.gene84742:CDS
GGATTATGACAGGGGAATAT+TGG 0.318844 8.4:+69554262 MS.gene84742:intron
TAGCCTACTTCAATTATATA+GGG 0.322498 8.4:+69555155 MS.gene84742:CDS
ACTGTCAAGCCATGAATAAT+GGG 0.326976 8.4:+69555365 MS.gene84742:CDS
AGCTTCTAGGATTGAAATAT+AGG 0.340995 8.4:-69555428 None:intergenic
AGAACTAAGGTCCTTGAATA+TGG 0.349233 8.4:+69555574 MS.gene84742:CDS
ATAGCCTACTTCAATTATAT+AGG 0.351392 8.4:+69555154 MS.gene84742:CDS
AAACTCTGGCCTGCTTGAAT+TGG 0.352309 8.4:-69553991 None:intergenic
AGACAGTCATGGATGGATTA+AGG 0.352838 8.4:+69555242 MS.gene84742:CDS
TGGCTGAGAGGAACGGTTCA+TGG 0.360623 8.4:+69554075 MS.gene84742:CDS
GTACCAACTTAACGTGATTC+CGG 0.363195 8.4:+69554877 MS.gene84742:CDS
TGTCAGAACCCCATTATTCA+TGG 0.367775 8.4:-69555374 None:intergenic
AAATTGAATTGTGCAGTTAG+TGG 0.377880 8.4:-69555712 None:intergenic
AGGGCCTGGATGACTGTTAA+TGG 0.379057 8.4:-69554358 None:intergenic
AATTGGACATTGTGTAATAA+AGG 0.383075 8.4:-69553974 None:intergenic
ATAATTATTTGCTCTTGATA+AGG 0.389280 8.4:-69554147 None:intergenic
CTCCAATTGTATTCATATAT+GGG 0.391433 8.4:-69555744 None:intergenic
GCCAGCTTATCATCAGGTTT+AGG 0.392466 8.4:-69555214 None:intergenic
CTTAACAAGTAGGTCCTTCC+CGG 0.393276 8.4:-69554896 None:intergenic
TATGAATACAATTGGAGTTC+CGG 0.398110 8.4:+69555750 MS.gene84742:CDS
TGATTGTTTATCCAAAGAAT+AGG 0.399902 8.4:+69554103 MS.gene84742:CDS
CCTTAACATGATGGCAGATC+TGG 0.409750 8.4:-69556131 None:intergenic
TTCAATGACTCCGTGTCATA+TGG 0.413446 8.4:-69555544 None:intergenic
GATTGTTTATCCAAAGAATA+GGG 0.418861 8.4:+69554104 MS.gene84742:CDS
TAGAACTTAGAGGGTGCATC+GGG 0.424101 8.4:-69555490 None:intergenic
GAAGAATGGAAAAGGAGAAC+TGG 0.425055 8.4:+69556088 MS.gene84742:CDS
ACATTGATATACACCAATCA+TGG 0.428766 8.4:+69556042 MS.gene84742:CDS
AACGAAGTCGTAGAACTTAG+AGG 0.434172 8.4:-69555500 None:intergenic
CCAGATCTGCCATCATGTTA+AGG 0.436349 8.4:+69556131 MS.gene84742:CDS
CTGTAGCCATGGATGTGAAT+TGG 0.439868 8.4:-69555652 None:intergenic
AACTGCACAATTCAATTTGG+TGG 0.440435 8.4:+69555717 MS.gene84742:CDS
CTTTACAGTGGTGAATCAAA+AGG 0.446706 8.4:+69554023 MS.gene84742:CDS
GCTTCTAGGATTGAAATATA+GGG 0.448642 8.4:-69555427 None:intergenic
TATTTGAGCAGTTGTAGTTA+GGG 0.448988 8.4:-69554377 None:intergenic
TACCAACTTAACGTGATTCC+GGG 0.449429 8.4:+69554878 MS.gene84742:CDS
ACAATCCTGCCAGACTTGTA+AGG 0.451834 8.4:-69555109 None:intergenic
AAAGACATTGTCACAGTCAA+TGG 0.458479 8.4:+69553727 MS.gene84742:CDS
CGGCTATCATGCTGGTATGA+TGG 0.460038 8.4:+69553946 MS.gene84742:CDS
TTGGAGTTCCGGTCGGTGGA+TGG 0.462730 8.4:+69555761 MS.gene84742:CDS
TTAACAGCCAGCTTATCATC+AGG 0.463496 8.4:-69555220 None:intergenic
ATTGAATGGAACTAGAACTA+AGG 0.468333 8.4:+69555561 MS.gene84742:CDS
CAAGAGCAAATAATTATACT+AGG 0.472623 8.4:+69554153 MS.gene84742:CDS
TCTTCTTGCGCGTAAACAAC+AGG 0.474363 8.4:-69553763 None:intergenic
ACCTAAACCTGATGATAAGC+TGG 0.474955 8.4:+69555213 MS.gene84742:CDS
TGCGCGTAAACAACAGGTCC+TGG 0.474976 8.4:-69553757 None:intergenic
CTTTCAATGTTACAATTCAC+TGG 0.476945 8.4:+69553821 MS.gene84742:CDS
GCTGTTGCACCTTACAAGTC+TGG 0.478773 8.4:+69555100 MS.gene84742:CDS
CTTGATAAGGAGCCTTAAAT+GGG 0.480376 8.4:-69554134 None:intergenic
GAGCAGTTGTAGTTAGGGCC+TGG 0.483325 8.4:-69554372 None:intergenic
TTTATAGTGAAGAATGGAAA+AGG 0.484441 8.4:+69556080 MS.gene84742:CDS
TGTAGCCATGGATGTGAATT+GGG 0.485014 8.4:-69555651 None:intergenic
ATGGTATTTATAGTGAAGAA+TGG 0.486058 8.4:+69556074 MS.gene84742:CDS
CTGGTCGATCAGCTGAAACT+AGG 0.488914 8.4:-69555060 None:intergenic
CCATTCCTAGTCCCCATGAT+TGG 0.490748 8.4:-69556055 None:intergenic
ATTCTTTGGATAAACAATCA+TGG 0.494706 8.4:-69554100 None:intergenic
TATGACACGGAGTCATTGAA+TGG 0.494839 8.4:+69555547 MS.gene84742:CDS
ATTTATTAGTCTTAACAAGT+AGG 0.500396 8.4:-69554906 None:intergenic
TCAGGTTTAGGTAATTTAGC+TGG 0.504745 8.4:-69555202 None:intergenic
CAGTGAATTGTAACATTGAA+AGG 0.505375 8.4:-69553820 None:intergenic
TACGTTGTCGGATATGGCAC+AGG 0.505791 8.4:+69555679 MS.gene84742:CDS
TGGAGTTCCGGTCGGTGGAT+GGG 0.506523 8.4:+69555762 MS.gene84742:CDS
ATCACTGTGTCTGTCGTGAA+TGG 0.510117 8.4:-69555010 None:intergenic
TTTACAGTGGTGAATCAAAA+GGG 0.516141 8.4:+69554024 MS.gene84742:CDS
TATATAATTGAAGTAGGCTA+TGG 0.522248 8.4:-69555152 None:intergenic
AACTTATGGCACATGGTGCA+AGG 0.522324 8.4:+69553516 None:intergenic
TTGTTAAGACTAATAAATGC+AGG 0.524233 8.4:+69554911 MS.gene84742:CDS
ACGACAGACACAGTGATGCT+TGG 0.527387 8.4:+69555016 MS.gene84742:CDS
TGTTTCTTGGCTGAGAGGAA+CGG 0.529635 8.4:+69554068 MS.gene84742:CDS
GACAGGGGAATATTGGCTGC+AGG 0.531339 8.4:+69554269 MS.gene84742:intron
TTAATGGTATATGCATCTGC+TGG 0.533299 8.4:-69554342 None:intergenic
AGGTCCTTGAATATGGTACC+AGG 0.534835 8.4:+69555581 MS.gene84742:CDS
TCCAATTGTATTCATATATG+GGG 0.536273 8.4:-69555743 None:intergenic
TTGCACCTTACAAGTCTGGC+AGG 0.539924 8.4:+69555104 MS.gene84742:CDS
TTCCCGGAATCACGTTAAGT+TGG 0.539938 8.4:-69554880 None:intergenic
CCAATCATGGGGACTAGGAA+TGG 0.541532 8.4:+69556055 MS.gene84742:CDS
ACAGTCAATGGTATGTATCC+AGG 0.543289 8.4:+69553739 MS.gene84742:CDS
TCCGGCAGCGGCTATCATGC+TGG 0.545473 8.4:+69553938 MS.gene84742:CDS
TAACATGATGGCAGATCTGG+TGG 0.545956 8.4:-69556128 None:intergenic
ACATGATGGCAGATCTGGTG+GGG 0.548065 8.4:-69556126 None:intergenic
CATTGATATACACCAATCAT+GGG 0.549375 8.4:+69556043 MS.gene84742:CDS
GCACCCTGGTACCATATTCA+AGG 0.552988 8.4:-69555585 None:intergenic
TATACCATTAACAGTCATCC+AGG 0.553014 8.4:+69554354 MS.gene84742:CDS
ACTTATGGCACATGGTGCAA+GGG 0.559084 8.4:+69553517 None:intergenic
TAAAGTTATAGGTGAAACTC+TGG 0.559122 8.4:-69554005 None:intergenic
TTAGGCATGGTATCCGGCAG+CGG 0.562296 8.4:+69553926 MS.gene84742:intron
ACAGCTGCCCATCCACCGAC+CGG 0.565254 8.4:-69555769 None:intergenic
GTATATGCATCTGCTGGAGG+TGG 0.568362 8.4:-69554336 None:intergenic
ACCAGCATGATAGCCGCTGC+CGG 0.569605 8.4:-69553939 None:intergenic
AGCTCCTCCGTTCACTAGAG+TGG 0.569778 8.4:-69554313 None:intergenic
TGTTAAGCTGTGATTAGCAA+TGG 0.570639 8.4:-69554957 None:intergenic
CTTGTAAGGTGCAACAGCCA+TGG 0.573214 8.4:-69555095 None:intergenic
TTTGTTTGGGTGTCTTTGAG+TGG 0.573318 8.4:-69555342 None:intergenic
CAACGTAAAAGCTGTAGCCA+TGG 0.575280 8.4:-69555663 None:intergenic
GTCGGTAGTCCTTAACATGA+TGG 0.577159 8.4:-69556140 None:intergenic
GCTCATGTTTCTTGGCTGAG+AGG 0.578994 8.4:+69554063 MS.gene84742:CDS
GGACATTGTGTAATAAAGGA+TGG 0.579344 8.4:-69553970 None:intergenic
ATACACCAATCATGGGGACT+AGG 0.580579 8.4:+69556050 MS.gene84742:CDS
GACACAGTGATGCTTGGACC+AGG 0.583332 8.4:+69555022 MS.gene84742:CDS
GCTGTTAAGACAGTCATGGA+TGG 0.584431 8.4:+69555235 MS.gene84742:CDS
AATCACCCAATTCACATCCA+TGG 0.590062 8.4:+69555646 MS.gene84742:CDS
GTAGAACTTAGAGGGTGCAT+CGG 0.590381 8.4:-69555491 None:intergenic
ACAATTGGAGTTCCGGTCGG+TGG 0.593840 8.4:+69555757 MS.gene84742:CDS
AACATGATGGCAGATCTGGT+GGG 0.594938 8.4:-69556127 None:intergenic
TAAGGAGCCTTAAATGGGAA+AGG 0.596691 8.4:-69554129 None:intergenic
CTTATGGCACATGGTGCAAG+GGG 0.596985 8.4:+69553518 None:intergenic
TTAACAATCGTGGAAGCTGA+TGG 0.598033 8.4:+69554974 MS.gene84742:CDS
ACACAATGTCCAATTCAAGC+AGG 0.599564 8.4:+69553982 MS.gene84742:CDS
GTTTCAGCTGATCGACCAGT+AGG 0.599812 8.4:+69555064 MS.gene84742:CDS
ACCAGTAGGAAAATATTCCA+TGG 0.612836 8.4:+69555078 MS.gene84742:CDS
TGCCCCTATATAATTGAAGT+AGG 0.615587 8.4:-69555158 None:intergenic
AGATGACAGAATCGTAGTCA+AGG 0.618005 8.4:+69553783 MS.gene84742:CDS
AGCCTACTTCAATTATATAG+GGG 0.623554 8.4:+69555156 MS.gene84742:CDS
ACGAAGTCGTAGAACTTAGA+GGG 0.623619 8.4:-69555499 None:intergenic
GAGCATGCCACTCTAGTGAA+CGG 0.631956 8.4:+69554306 MS.gene84742:CDS
GCTGGCTGTTAAGACAGTCA+TGG 0.632833 8.4:+69555231 MS.gene84742:CDS
CATGATGGCAGATCTGGTGG+GGG 0.639539 8.4:-69556125 None:intergenic
AGGACATTCAGTGTTTGACC+TGG 0.640728 8.4:-69555040 None:intergenic
AACTTAACGTGATTCCGGGA+AGG 0.644475 8.4:+69554882 MS.gene84742:CDS
CTGTCAAGCCATGAATAATG+GGG 0.645622 8.4:+69555366 MS.gene84742:CDS
TATGTCAAACTTATGGCACA+TGG 0.655710 8.4:+69553509 None:intergenic
CCAATTGTATTCATATATGG+GGG 0.658094 8.4:-69555742 None:intergenic
TAATCACAGCTTAACAATCG+TGG 0.664550 8.4:+69554964 MS.gene84742:CDS
ATGGTATATGCATCTGCTGG+AGG 0.664774 8.4:-69554339 None:intergenic
GTCATATGGAATGTTATTAG+GGG 0.668087 8.4:-69555530 None:intergenic
TTTCACCTATAACTTTACAG+TGG 0.669501 8.4:+69554011 MS.gene84742:CDS
AATACAATTGGAGTTCCGGT+CGG 0.670994 8.4:+69555754 MS.gene84742:CDS
GGTCCTTGAATATGGTACCA+GGG 0.679440 8.4:+69555582 MS.gene84742:CDS
CTGCAAAATGAGTTGCACCC+TGG 0.684133 8.4:-69555599 None:intergenic
TAATAACATTCCATATGACA+CGG 0.686240 8.4:+69555534 MS.gene84742:CDS
GTGCAAGGGGAGAATCAACA+AGG 0.699031 8.4:+69553531 MS.gene84742:CDS
ATTGATATACACCAATCATG+GGG 0.703520 8.4:+69556044 MS.gene84742:CDS
AATAAATGCAGGTTTAAACA+CGG 0.710080 8.4:+69554922 MS.gene84742:CDS
CATGCCACTCTAGTGAACGG+AGG 0.736091 8.4:+69554309 MS.gene84742:CDS
ATGATTGGTGTATATCAATG+TGG 0.739350 8.4:-69556040 None:intergenic
AGAACTTAGAGGGTGCATCG+GGG 0.784339 8.4:-69555489 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATATTTCTATTTATTTG+TGG + chr8.4:69553609-69553628 MS.gene84742:intron 10.0%
!!! CTATTTTTTAAAATTATTAC+TGG + chr8.4:69554566-69554585 MS.gene84742:intron 10.0%
!! GCTTATTATAAGAAAATAAA+TGG + chr8.4:69555448-69555467 MS.gene84742:CDS 15.0%
!! TTAAGTTACATCAAATAATT+TGG + chr8.4:69554701-69554720 MS.gene84742:intron 15.0%
!!! AAAATACTGATTTCATTTAA+TGG + chr8.4:69554444-69554463 MS.gene84742:intron 15.0%
!!! AAATACTGATTTCATTTAAT+GGG + chr8.4:69554445-69554464 MS.gene84742:intron 15.0%
!!! TATTTCTTTTTTTTTCTCTA+TGG + chr8.4:69555950-69555969 MS.gene84742:intron 15.0%
!! AAAAATATATTCATCACCAT+TGG + chr8.4:69555301-69555320 MS.gene84742:CDS 20.0%
!! AATTATACTAGGTAAGTTAA+AGG + chr8.4:69554164-69554183 MS.gene84742:intron 20.0%
!! ATAATTATTTGCTCTTGATA+AGG - chr8.4:69554150-69554169 None:intergenic 20.0%
!!! AAACTTGATTTTCGAATTTT+AGG + chr8.4:69553908-69553927 MS.gene84742:intron 20.0%
!!! AATACTGATTTCATTTAATG+GGG + chr8.4:69554446-69554465 MS.gene84742:intron 20.0%
!!! ATTTATTAGTCTTAACAAGT+AGG - chr8.4:69554909-69554928 None:intergenic 20.0%
!!! CTTTTAAAACAAATGTTAGT+GGG - chr8.4:69555916-69555935 None:intergenic 20.0%
!!! TATTTTACTGAAACATTTCA+GGG + chr8.4:69555845-69555864 MS.gene84742:intron 20.0%
!!! TATTTTTTCTACAAATGTGA+AGG - chr8.4:69553595-69553614 None:intergenic 20.0%
!!! TCTTTTAAAACAAATGTTAG+TGG - chr8.4:69555917-69555936 None:intergenic 20.0%
!!! TTTTCTTATAATAAGCTTCT+AGG - chr8.4:69555444-69555463 None:intergenic 20.0%
! AATAAATGCAGGTTTAAACA+CGG + chr8.4:69554922-69554941 MS.gene84742:CDS 25.0%
! AATTGGACATTGTGTAATAA+AGG - chr8.4:69553977-69553996 None:intergenic 25.0%
! ACTCCAATTGTATTCATATA+TGG - chr8.4:69555748-69555767 None:intergenic 25.0%
! ATAGCCTACTTCAATTATAT+AGG + chr8.4:69555154-69555173 MS.gene84742:CDS 25.0%
! ATCAAATAATTTGGCTAACA+TGG + chr8.4:69554710-69554729 MS.gene84742:intron 25.0%
! ATGGTATTTATAGTGAAGAA+TGG + chr8.4:69556074-69556093 MS.gene84742:CDS 25.0%
! ATTCTTTGGATAAACAATCA+TGG - chr8.4:69554103-69554122 None:intergenic 25.0%
! CAAGAGCAAATAATTATACT+AGG + chr8.4:69554153-69554172 MS.gene84742:CDS 25.0%
! CTCCAATTGTATTCATATAT+GGG - chr8.4:69555747-69555766 None:intergenic 25.0%
! GATTGTTTATCCAAAGAATA+GGG + chr8.4:69554104-69554123 MS.gene84742:CDS 25.0%
! TAATAACATTCCATATGACA+CGG + chr8.4:69555534-69555553 MS.gene84742:CDS 25.0%
! TAGCCTACTTCAATTATATA+GGG + chr8.4:69555155-69555174 MS.gene84742:CDS 25.0%
! TATATAATTGAAGTAGGCTA+TGG - chr8.4:69555155-69555174 None:intergenic 25.0%
! TCCAATTGTATTCATATATG+GGG - chr8.4:69555746-69555765 None:intergenic 25.0%
! TGATTGTTTATCCAAAGAAT+AGG + chr8.4:69554103-69554122 MS.gene84742:CDS 25.0%
! TGTCATATGGAATGTTATTA+GGG - chr8.4:69555534-69555553 None:intergenic 25.0%
! TTGTTAAGACTAATAAATGC+AGG + chr8.4:69554911-69554930 MS.gene84742:CDS 25.0%
! TTTATAGTGAAGAATGGAAA+AGG + chr8.4:69556080-69556099 MS.gene84742:CDS 25.0%
!! AACAAGGTTCTATGATTTTA+AGG + chr8.4:69553547-69553566 MS.gene84742:CDS 25.0%
!! CATAGGTTGTATACAATTTT+AGG - chr8.4:69554476-69554495 None:intergenic 25.0%
!! CTATTTTACTGAAACATTTC+AGG + chr8.4:69555844-69555863 MS.gene84742:intron 25.0%
!! CTTTTTAACATTCAATCCAA+TGG - chr8.4:69555320-69555339 None:intergenic 25.0%
!!! ACTATTTTTGATGTGCTTAA+TGG + chr8.4:69553657-69553676 MS.gene84742:intron 25.0%
!!! ATACTGATTTCATTTAATGG+GGG + chr8.4:69554447-69554466 MS.gene84742:intron 25.0%
!!! TTTTTTTTCTCTATGGCTAA+AGG + chr8.4:69555957-69555976 MS.gene84742:intron 25.0%
AAACACTGAATTCAAACCAA+AGG - chr8.4:69554195-69554214 None:intergenic 30.0%
AAATTGAATTGTGCAGTTAG+TGG - chr8.4:69555715-69555734 None:intergenic 30.0%
AACTACAACTGCTCAAATAA+TGG + chr8.4:69554381-69554400 MS.gene84742:CDS 30.0%
AATGAACTGCATTATGTATG+CGG - chr8.4:69553862-69553881 None:intergenic 30.0%
ACATTGATATACACCAATCA+TGG + chr8.4:69556042-69556061 MS.gene84742:CDS 30.0%
ACCTGCAAAAAGTTATGTTA+CGG - chr8.4:69556003-69556022 None:intergenic 30.0%
ACTAACTGCACAATTCAATT+TGG + chr8.4:69555714-69555733 MS.gene84742:CDS 30.0%
AGCCTACTTCAATTATATAG+GGG + chr8.4:69555156-69555175 MS.gene84742:CDS 30.0%
ATGATTGGTGTATATCAATG+TGG - chr8.4:69556043-69556062 None:intergenic 30.0%
ATTCACCACTGTAAAGTTAT+AGG - chr8.4:69554019-69554038 None:intergenic 30.0%
ATTGAATGGAACTAGAACTA+AGG + chr8.4:69555561-69555580 MS.gene84742:CDS 30.0%
ATTGATATACACCAATCATG+GGG + chr8.4:69556044-69556063 MS.gene84742:CDS 30.0%
CAGTGAATTGTAACATTGAA+AGG - chr8.4:69553823-69553842 None:intergenic 30.0%
CATTGATATACACCAATCAT+GGG + chr8.4:69556043-69556062 MS.gene84742:CDS 30.0%
CCAATTGTATTCATATATGG+GGG - chr8.4:69555745-69555764 None:intergenic 30.0%
CTTTCAATGTTACAATTCAC+TGG + chr8.4:69553821-69553840 MS.gene84742:CDS 30.0%
GTCATATGGAATGTTATTAG+GGG - chr8.4:69555533-69555552 None:intergenic 30.0%
GTGTCATATGGAATGTTATT+AGG - chr8.4:69555535-69555554 None:intergenic 30.0%
TAAAGTTATAGGTGAAACTC+TGG - chr8.4:69554008-69554027 None:intergenic 30.0%
TAGTCTCATGGATATTGTTA+TGG - chr8.4:69554775-69554794 None:intergenic 30.0%
TATACATTGAGATGTATACC+TGG - chr8.4:69555814-69555833 None:intergenic 30.0%
TATACTAGGTAAGTTAAAGG+TGG + chr8.4:69554167-69554186 MS.gene84742:intron 30.0%
TATGAATACAATTGGAGTTC+CGG + chr8.4:69555750-69555769 MS.gene84742:CDS 30.0%
TATTTGAGCAGTTGTAGTTA+GGG - chr8.4:69554380-69554399 None:intergenic 30.0%
TTATTTGAGCAGTTGTAGTT+AGG - chr8.4:69554381-69554400 None:intergenic 30.0%
TTTACAGTGGTGAATCAAAA+GGG + chr8.4:69554024-69554043 MS.gene84742:CDS 30.0%
TTTCACCTATAACTTTACAG+TGG + chr8.4:69554011-69554030 MS.gene84742:CDS 30.0%
! TACACATCTGAAAAGTTGAT+AGG - chr8.4:69554860-69554879 None:intergenic 30.0%
! TGATTTTCGAATTTTAGGCA+TGG + chr8.4:69553913-69553932 MS.gene84742:intron 30.0%
! TTCAATATGAAGTGTCAATG+TGG - chr8.4:69554499-69554518 None:intergenic 30.0%
!! AACATTGAAAGGTGTCTTAT+TGG - chr8.4:69553812-69553831 None:intergenic 30.0%
!! AGCTTCTAGGATTGAAATAT+AGG - chr8.4:69555431-69555450 None:intergenic 30.0%
!! ATGTTAGTGGGTAATTTGTT+AGG - chr8.4:69555904-69555923 None:intergenic 30.0%
!! GCTTCTAGGATTGAAATATA+GGG - chr8.4:69555430-69555449 None:intergenic 30.0%
!! TGTTAGTGGGTAATTTGTTA+GGG - chr8.4:69555903-69555922 None:intergenic 30.0%
AAAGACATTGTCACAGTCAA+TGG + chr8.4:69553727-69553746 MS.gene84742:CDS 35.0%
AACTGCACAATTCAATTTGG+TGG + chr8.4:69555717-69555736 MS.gene84742:CDS 35.0%
AACTGTCAAGCCATGAATAA+TGG + chr8.4:69555364-69555383 MS.gene84742:CDS 35.0%
AAGTTCTACGACTTCGTTAA+TGG + chr8.4:69555505-69555524 MS.gene84742:CDS 35.0%
ACCAGTAGGAAAATATTCCA+TGG + chr8.4:69555078-69555097 MS.gene84742:CDS 35.0%
ACTGTCAAGCCATGAATAAT+GGG + chr8.4:69555365-69555384 MS.gene84742:CDS 35.0%
AGAACTAAGGTCCTTGAATA+TGG + chr8.4:69555574-69555593 MS.gene84742:CDS 35.0%
AGCTAATTCGGATTATGACA+GGG + chr8.4:69554253-69554272 MS.gene84742:intron 35.0%
CCCCCATATATGAATACAAT+TGG + chr8.4:69555742-69555761 MS.gene84742:CDS 35.0%
CTTGATAAGGAGCCTTAAAT+GGG - chr8.4:69554137-69554156 None:intergenic 35.0%
CTTTACAGTGGTGAATCAAA+AGG + chr8.4:69554023-69554042 MS.gene84742:CDS 35.0%
GGACATTGTGTAATAAAGGA+TGG - chr8.4:69553973-69553992 None:intergenic 35.0%
TAAGTTAAAGGTGGTTCCTT+TGG + chr8.4:69554176-69554195 MS.gene84742:intron 35.0%
TAATCACAGCTTAACAATCG+TGG + chr8.4:69554964-69554983 MS.gene84742:CDS 35.0%
TACAAATGTGAAGGTCAGAA+TGG - chr8.4:69553586-69553605 None:intergenic 35.0%
TATACCATTAACAGTCATCC+AGG + chr8.4:69554354-69554373 MS.gene84742:CDS 35.0%
TCAGGTTTAGGTAATTTAGC+TGG - chr8.4:69555205-69555224 None:intergenic 35.0%
TCTTGATAAGGAGCCTTAAA+TGG - chr8.4:69554138-69554157 None:intergenic 35.0%
TGCCCCTATATAATTGAAGT+AGG - chr8.4:69555161-69555180 None:intergenic 35.0%
TGTATCCATTGTGTTAGTGT+CGG - chr8.4:69554536-69554555 None:intergenic 35.0%
TGTTAAGCTGTGATTAGCAA+TGG - chr8.4:69554960-69554979 None:intergenic 35.0%
TTAATGGTATATGCATCTGC+TGG - chr8.4:69554345-69554364 None:intergenic 35.0%
! CTTCATATTGAAAGCATGTC+CGG + chr8.4:69554507-69554526 MS.gene84742:intron 35.0%
! TCCGTAACATAACTTTTTGC+AGG + chr8.4:69555999-69556018 MS.gene84742:intron 35.0%
!! GTTAGTGGGTAATTTGTTAG+GGG - chr8.4:69555902-69555921 None:intergenic 35.0%
!! TTAGTGGGTAATTTGTTAGG+GGG - chr8.4:69555901-69555920 None:intergenic 35.0%
!!! ATCAAAAGGGCACTTTCTTT+TGG + chr8.4:69554037-69554056 MS.gene84742:CDS 35.0%
!!! ATGGCTTGACAGTTTTGTTT+GGG - chr8.4:69555358-69555377 None:intergenic 35.0%
AACGAAGTCGTAGAACTTAG+AGG - chr8.4:69555503-69555522 None:intergenic 40.0%
AATACAATTGGAGTTCCGGT+CGG + chr8.4:69555754-69555773 MS.gene84742:CDS 40.0%
AATCACCCAATTCACATCCA+TGG + chr8.4:69555646-69555665 MS.gene84742:CDS 40.0%
AATTTGGCTAACATGGTGCA+TGG + chr8.4:69554717-69554736 MS.gene84742:intron 40.0%
ACACAATGTCCAATTCAAGC+AGG + chr8.4:69553982-69554001 MS.gene84742:CDS 40.0%
ACAGTCAATGGTATGTATCC+AGG + chr8.4:69553739-69553758 MS.gene84742:CDS 40.0%
ACCTAAACCTGATGATAAGC+TGG + chr8.4:69555213-69555232 MS.gene84742:CDS 40.0%
ACGAAGTCGTAGAACTTAGA+GGG - chr8.4:69555502-69555521 None:intergenic 40.0%
ACTGATGTTGCGAGCTAATT+CGG + chr8.4:69554241-69554260 MS.gene84742:intron 40.0%
AGATGACAGAATCGTAGTCA+AGG + chr8.4:69553783-69553802 MS.gene84742:CDS 40.0%
AGTCTGTGTTAGTGCTTCAT+AGG + chr8.4:69554627-69554646 MS.gene84742:intron 40.0%
CTGTCAAGCCATGAATAATG+GGG + chr8.4:69555366-69555385 MS.gene84742:CDS 40.0%
GAAGAATGGAAAAGGAGAAC+TGG + chr8.4:69556088-69556107 MS.gene84742:CDS 40.0%
GAGCTAATTCGGATTATGAC+AGG + chr8.4:69554252-69554271 MS.gene84742:intron 40.0%
GCTAATTCGGATTATGACAG+GGG + chr8.4:69554254-69554273 MS.gene84742:intron 40.0%
GGATTATGACAGGGGAATAT+TGG + chr8.4:69554262-69554281 MS.gene84742:intron 40.0%
GTACCAACTTAACGTGATTC+CGG + chr8.4:69554877-69554896 MS.gene84742:CDS 40.0%
GTGCTTAATGGTTACTGTAC+AGG + chr8.4:69553669-69553688 MS.gene84742:intron 40.0%
TAAGGAGCCTTAAATGGGAA+AGG - chr8.4:69554132-69554151 None:intergenic 40.0%
TACCAACTTAACGTGATTCC+GGG + chr8.4:69554878-69554897 MS.gene84742:CDS 40.0%
TATGACACGGAGTCATTGAA+TGG + chr8.4:69555547-69555566 MS.gene84742:CDS 40.0%
TGTAGCCATGGATGTGAATT+GGG - chr8.4:69555654-69555673 None:intergenic 40.0%
TGTCAGAACCCCATTATTCA+TGG - chr8.4:69555377-69555396 None:intergenic 40.0%
TTAACAATCGTGGAAGCTGA+TGG + chr8.4:69554974-69554993 MS.gene84742:CDS 40.0%
TTAACAGCCAGCTTATCATC+AGG - chr8.4:69555223-69555242 None:intergenic 40.0%
TTCAATGACTCCGTGTCATA+TGG - chr8.4:69555547-69555566 None:intergenic 40.0%
TTGTGAGTTACGTAGTCTCA+TGG - chr8.4:69554787-69554806 None:intergenic 40.0%
TTTGGCATGCTCATGTTTCT+TGG + chr8.4:69554055-69554074 MS.gene84742:CDS 40.0%
TTTGTTTGGGTGTCTTTGAG+TGG - chr8.4:69555345-69555364 None:intergenic 40.0%
! AGCTTTTACGTTGTCGGATA+TGG + chr8.4:69555673-69555692 MS.gene84742:CDS 40.0%
! ATGAAGTGTCAATGTGGCAT+AGG - chr8.4:69554493-69554512 None:intergenic 40.0%
! CAACTCATTTTGCAGAACAC+AGG + chr8.4:69555607-69555626 MS.gene84742:CDS 40.0%
! CATAACTTTTTGCAGGTGTG+TGG + chr8.4:69556006-69556025 MS.gene84742:intron 40.0%
! CGAATTTTAGGCATGGTATC+CGG + chr8.4:69553920-69553939 MS.gene84742:intron 40.0%
! CGTTTTGTAGCTGATAATCC+AGG + chr8.4:69555793-69555812 MS.gene84742:CDS 40.0%
! GCCATGGAATATTTTCCTAC+TGG - chr8.4:69555082-69555101 None:intergenic 40.0%
! TAGGGTACCTTTCCCATTTA+AGG + chr8.4:69554122-69554141 MS.gene84742:CDS 40.0%
!! AGACAGTCATGGATGGATTA+AGG + chr8.4:69555242-69555261 MS.gene84742:CDS 40.0%
!!! CATGGCTTGACAGTTTTGTT+TGG - chr8.4:69555359-69555378 None:intergenic 40.0%
AAACTCTGGCCTGCTTGAAT+TGG - chr8.4:69553994-69554013 None:intergenic 45.0%
AACATGATGGCAGATCTGGT+GGG - chr8.4:69556130-69556149 None:intergenic 45.0%
AACTTAACGTGATTCCGGGA+AGG + chr8.4:69554882-69554901 MS.gene84742:CDS 45.0%
ACAATCCTGCCAGACTTGTA+AGG - chr8.4:69555112-69555131 None:intergenic 45.0%
AGGACATTCAGTGTTTGACC+TGG - chr8.4:69555043-69555062 None:intergenic 45.0%
AGGTCCTTGAATATGGTACC+AGG + chr8.4:69555581-69555600 MS.gene84742:CDS 45.0%
ATACACCAATCATGGGGACT+AGG + chr8.4:69556050-69556069 MS.gene84742:CDS 45.0%
ATCACTGTGTCTGTCGTGAA+TGG - chr8.4:69555013-69555032 None:intergenic 45.0%
ATGGTATATGCATCTGCTGG+AGG - chr8.4:69554342-69554361 None:intergenic 45.0%
CAACGTAAAAGCTGTAGCCA+TGG - chr8.4:69555666-69555685 None:intergenic 45.0%
CTGTAGCCATGGATGTGAAT+TGG - chr8.4:69555655-69555674 None:intergenic 45.0%
CTTAACAAGTAGGTCCTTCC+CGG - chr8.4:69554899-69554918 None:intergenic 45.0%
GCCAGCTTATCATCAGGTTT+AGG - chr8.4:69555217-69555236 None:intergenic 45.0%
GCTGTTAAGACAGTCATGGA+TGG + chr8.4:69555235-69555254 MS.gene84742:CDS 45.0%
GGTCCTTGAATATGGTACCA+GGG + chr8.4:69555582-69555601 MS.gene84742:CDS 45.0%
TAACATGATGGCAGATCTGG+TGG - chr8.4:69556131-69556150 None:intergenic 45.0%
TCTTCTTGCGCGTAAACAAC+AGG - chr8.4:69553766-69553785 None:intergenic 45.0%
TGTTTCTTGGCTGAGAGGAA+CGG + chr8.4:69554068-69554087 MS.gene84742:CDS 45.0%
TTCCCGGAATCACGTTAAGT+TGG - chr8.4:69554883-69554902 None:intergenic 45.0%
! GGCTACAGCTTTTACGTTGT+CGG + chr8.4:69555667-69555686 MS.gene84742:CDS 45.0%
! GTAGAACTTAGAGGGTGCAT+CGG - chr8.4:69555494-69555513 None:intergenic 45.0%
! TAGAACTTAGAGGGTGCATC+GGG - chr8.4:69555493-69555512 None:intergenic 45.0%
!! GGGAAAGGTACCCTATTCTT+TGG - chr8.4:69554117-69554136 None:intergenic 45.0%
!! TGGGTAATTTGTTAGGGGGA+GGG - chr8.4:69555897-69555916 None:intergenic 45.0%
ACATGATGGCAGATCTGGTG+GGG - chr8.4:69556129-69556148 None:intergenic 50.0%
ACGACAGACACAGTGATGCT+TGG + chr8.4:69555016-69555035 MS.gene84742:CDS 50.0%
AGGGCCTGGATGACTGTTAA+TGG - chr8.4:69554361-69554380 None:intergenic 50.0%
CCAATCATGGGGACTAGGAA+TGG + chr8.4:69556055-69556074 MS.gene84742:CDS 50.0%
CCATTCCTAGTCCCCATGAT+TGG - chr8.4:69556058-69556077 None:intergenic 50.0%
CGGCTATCATGCTGGTATGA+TGG + chr8.4:69553946-69553965 MS.gene84742:CDS 50.0%
CTGCAAAATGAGTTGCACCC+TGG - chr8.4:69555602-69555621 None:intergenic 50.0%
CTGGTCGATCAGCTGAAACT+AGG - chr8.4:69555063-69555082 None:intergenic 50.0%
CTTGTAAGGTGCAACAGCCA+TGG - chr8.4:69555098-69555117 None:intergenic 50.0%
GAGCATGCCACTCTAGTGAA+CGG + chr8.4:69554306-69554325 MS.gene84742:CDS 50.0%
GCACCCTGGTACCATATTCA+AGG - chr8.4:69555588-69555607 None:intergenic 50.0%
GCTCATGTTTCTTGGCTGAG+AGG + chr8.4:69554063-69554082 MS.gene84742:CDS 50.0%
GCTGGCTGTTAAGACAGTCA+TGG + chr8.4:69555231-69555250 MS.gene84742:CDS 50.0%
GCTGTTGCACCTTACAAGTC+TGG + chr8.4:69555100-69555119 MS.gene84742:CDS 50.0%
GTATATGCATCTGCTGGAGG+TGG - chr8.4:69554339-69554358 None:intergenic 50.0%
GTGCAAGGGGAGAATCAACA+AGG + chr8.4:69553531-69553550 MS.gene84742:CDS 50.0%
GTTTCAGCTGATCGACCAGT+AGG + chr8.4:69555064-69555083 MS.gene84742:CDS 50.0%
TACGTTGTCGGATATGGCAC+AGG + chr8.4:69555679-69555698 MS.gene84742:CDS 50.0%
TTGCACCTTACAAGTCTGGC+AGG + chr8.4:69555104-69555123 MS.gene84742:CDS 50.0%
TTGGCTAACATGGTGCATGG+TGG + chr8.4:69554720-69554739 MS.gene84742:intron 50.0%
! AGAACTTAGAGGGTGCATCG+GGG - chr8.4:69555492-69555511 None:intergenic 50.0%
! ATTGTGTTAGTGTCGGACGC+CGG - chr8.4:69554529-69554548 None:intergenic 50.0%
!! GGGTAATTTGTTAGGGGGAG+GGG - chr8.4:69555896-69555915 None:intergenic 50.0%
!! GGTAATTTGTTAGGGGGAGG+GGG - chr8.4:69555895-69555914 None:intergenic 50.0%
!! GTAATTTGTTAGGGGGAGGG+GGG - chr8.4:69555894-69555913 None:intergenic 50.0%
!! GTGGGTAATTTGTTAGGGGG+AGG - chr8.4:69555898-69555917 None:intergenic 50.0%
ACAATTGGAGTTCCGGTCGG+TGG + chr8.4:69555757-69555776 MS.gene84742:CDS 55.0%
AGCTCCTCCGTTCACTAGAG+TGG - chr8.4:69554316-69554335 None:intergenic 55.0%
CATGATGGCAGATCTGGTGG+GGG - chr8.4:69556128-69556147 None:intergenic 55.0%
CATGCCACTCTAGTGAACGG+AGG + chr8.4:69554309-69554328 MS.gene84742:CDS 55.0%
GACACAGTGATGCTTGGACC+AGG + chr8.4:69555022-69555041 MS.gene84742:CDS 55.0%
GAGCAGTTGTAGTTAGGGCC+TGG - chr8.4:69554375-69554394 None:intergenic 55.0%
GGCGTCCGACACTAACACAA+TGG + chr8.4:69554528-69554547 MS.gene84742:intron 55.0%
TGCGCGTAAACAACAGGTCC+TGG - chr8.4:69553760-69553779 None:intergenic 55.0%
TGGCTGAGAGGAACGGTTCA+TGG + chr8.4:69554075-69554094 MS.gene84742:CDS 55.0%
TTAGGCATGGTATCCGGCAG+CGG + chr8.4:69553926-69553945 MS.gene84742:intron 55.0%
! GACAGGGGAATATTGGCTGC+AGG + chr8.4:69554269-69554288 MS.gene84742:intron 55.0%
ACCAGCATGATAGCCGCTGC+CGG - chr8.4:69553942-69553961 None:intergenic 60.0%
TGGAGTTCCGGTCGGTGGAT+GGG + chr8.4:69555762-69555781 MS.gene84742:CDS 60.0%
TTGGAGTTCCGGTCGGTGGA+TGG + chr8.4:69555761-69555780 MS.gene84742:CDS 60.0%
ACAGCTGCCCATCCACCGAC+CGG - chr8.4:69555772-69555791 None:intergenic 65.0%
GGCAGATCTGGTGGGGGATG+TGG - chr8.4:69556122-69556141 None:intergenic 65.0%
TCCGGCAGCGGCTATCATGC+TGG + chr8.4:69553938-69553957 MS.gene84742:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 69553521 69556151 69553521 ID=MS.gene84742
chr8.4 mRNA 69553521 69556151 69553521 ID=MS.gene84742.t1;Parent=MS.gene84742
chr8.4 exon 69553521 69553568 69553521 ID=MS.gene84742.t1.exon1;Parent=MS.gene84742.t1
chr8.4 CDS 69553521 69553568 69553521 ID=cds.MS.gene84742.t1;Parent=MS.gene84742.t1
chr8.4 exon 69553691 69553842 69553691 ID=MS.gene84742.t1.exon2;Parent=MS.gene84742.t1
chr8.4 CDS 69553691 69553842 69553691 ID=cds.MS.gene84742.t1;Parent=MS.gene84742.t1
chr8.4 exon 69553930 69554174 69553930 ID=MS.gene84742.t1.exon3;Parent=MS.gene84742.t1
chr8.4 CDS 69553930 69554174 69553930 ID=cds.MS.gene84742.t1;Parent=MS.gene84742.t1
chr8.4 exon 69554274 69554402 69554274 ID=MS.gene84742.t1.exon4;Parent=MS.gene84742.t1
chr8.4 CDS 69554274 69554402 69554274 ID=cds.MS.gene84742.t1;Parent=MS.gene84742.t1
chr8.4 exon 69554873 69555814 69554873 ID=MS.gene84742.t1.exon5;Parent=MS.gene84742.t1
chr8.4 CDS 69554873 69555814 69554873 ID=cds.MS.gene84742.t1;Parent=MS.gene84742.t1
chr8.4 exon 69556021 69556151 69556021 ID=MS.gene84742.t1.exon6;Parent=MS.gene84742.t1
chr8.4 CDS 69556021 69556151 69556021 ID=cds.MS.gene84742.t1;Parent=MS.gene84742.t1
Gene Sequence

>MS.gene84742

ATGGCACATGGTGCAAGGGGAGAATCAACAAGGTTCTATGATTTTAAGGTATTTTCTTTGTTCCATTCTGACCTTCACATTTGTAGAAAAAATATTTCTATTTATTTGTGGTTTTCAAAATTCAAAAGCAATATTAACTATTTTTGATGTGCTTAATGGTTACTGTACAGGTTCAAACTAAAAGAGTTGCAAAACTTTGCAACTCAAAAGACATTGTCACAGTCAATGGTATGTATCCAGGACCTGTTGTTTACGCGCAAGAAGATGACAGAATCGTAGTCAAGGTTACCAATAAGACACCTTTCAATGTTACAATTCACTGGTAATTTCAAGCAATACCGCATACATAATGCAGTTCATTTAATAAAAAATGATAATTTAACTAACAAACTTGATTTTCGAATTTTAGGCATGGTATCCGGCAGCGGCTATCATGCTGGTATGATGGACCATCCTTTATTACACAATGTCCAATTCAAGCAGGCCAGAGTTTCACCTATAACTTTACAGTGGTGAATCAAAAGGGCACTTTCTTTTGGCATGCTCATGTTTCTTGGCTGAGAGGAACGGTTCATGGAGCCATGATTGTTTATCCAAAGAATAGGGTACCTTTCCCATTTAAGGCTCCTTATCAAGAGCAAATAATTATACTAGGTAAGTTAAAGGTGGTTCCTTTGGTTTGAATTCAGTGTTTCAAGTTTTATGTTTTCTCTATACAACACTGATGTTGCGAGCTAATTCGGATTATGACAGGGGAATATTGGCTGCAGGATCTTCAACAAATTGAGCATGCCACTCTAGTGAACGGAGGAGCTCCACCTCCAGCAGATGCATATACCATTAACAGTCATCCAGGCCCTAACTACAACTGCTCAAATAATGGTATTTTTGAGATAAAAGAAGATAAATAATAAGTGATCGTGAAAATACTGATTTCATTTAATGGGGGTTTCCTAAAATTGTATACAACCTATGCCACATTGACACTTCATATTGAAAGCATGTCCGGCGTCCGACACTAACACAATGGATACACACAATCATTCTATTTTTTAAAATTATTACTGGTCTCTACGTGTATGTGTCAATGTCGTTTTTGATGTTTGAGTCTGTGTTAGTGCTTCATAGGATACAACTAACAATGTCAGTGATTATTTTATAAAGTGATGTTTCAATCAGATTAAGTTACATCAAATAATTTGGCTAACATGGTGCATGGTGGTAATCTTTTTCACTAAGAATCTATTGACACCATAACAATATCCATGAGACTACGTAACTCACAACTTTGTAATTTAAATTTTCTATCTTCAGTTTTCACTGATATGTTTCATTTCCTATCAACTTTTCAGATGTGTACCAACTTAACGTGATTCCGGGAAGGACCTACTTGTTAAGACTAATAAATGCAGGTTTAAACACGGAGAACTTCTTTGCCATTGCTAATCACAGCTTAACAATCGTGGAAGCTGATGGTGAATACACGAAGCCATTCACGACAGACACAGTGATGCTTGGACCAGGTCAAACACTGAATGTCCTAGTTTCAGCTGATCGACCAGTAGGAAAATATTCCATGGCTGTTGCACCTTACAAGTCTGGCAGGATTGTCAAATATCAAAATGTTTCAGCCATAGCCTACTTCAATTATATAGGGGCAGAATCTAACAGTTCGTATTTACCAGCTAAATTACCTAAACCTGATGATAAGCTGGCTGTTAAGACAGTCATGGATGGATTAAGGAGCTTAAATCAAGTGAACGTTTTCAAAGAAATTGATAAAAATATATTCATCACCATTGGATTGAATGTTAAAAAGTGCCACTCAAAGACACCCAAACAAAACTGTCAAGCCATGAATAATGGGGTTCTGACAGCTTCAATGAACAATATAAGTTTCATTCATCCCTATATTTCAATCCTAGAAGCTTATTATAAGAAAATAAATGGATCGTATACTGAAGATTTCCCCGATGCACCCTCTAAGTTCTACGACTTCGTTAATGGTGCCCCTAATAACATTCCATATGACACGGAGTCATTGAATGGAACTAGAACTAAGGTCCTTGAATATGGTACCAGGGTGCAACTCATTTTGCAGAACACAGGAACAGTCAACATTGAAAATCACCCAATTCACATCCATGGCTACAGCTTTTACGTTGTCGGATATGGCACAGGCAACTACAATCCACTAACTGCACAATTCAATTTGGTGGATCCCCCATATATGAATACAATTGGAGTTCCGGTCGGTGGATGGGCAGCTGTTCGTTTTGTAGCTGATAATCCAGGTATACATCTCAATGTATATTTCTTTTTTCTATTTTACTGAAACATTTCAGGGTTCGATCTCTGTTGCTAACAAATCCCCCCTCCCCCTAACAAATTACCCACTAACATTTGTTTTAAAAGATCAAGAAACTGTTTATTTCTTTTTTTTTCTCTATGGCTAAAGGAATAATTCATTTTTTATAATCCGTAACATAACTTTTTGCAGGTGTGTGGTACATGCACTGCCACATTGATATACACCAATCATGGGGACTAGGAATGGTATTTATAGTGAAGAATGGAAAAGGAGAACTGGAGTCTTTACCACATCCCCCACCAGATCTGCCATCATGTTAA
Protein sequence

>MS.gene84742.t1

MAHGARGESTRFYDFKVQTKRVAKLCNSKDIVTVNGMYPGPVVYAQEDDRIVVKVTNKTPFNVTIHWHGIRQRLSCWYDGPSFITQCPIQAGQSFTYNFTVVNQKGTFFWHAHVSWLRGTVHGAMIVYPKNRVPFPFKAPYQEQIIILGEYWLQDLQQIEHATLVNGGAPPPADAYTINSHPGPNYNCSNNDVYQLNVIPGRTYLLRLINAGLNTENFFAIANHSLTIVEADGEYTKPFTTDTVMLGPGQTLNVLVSADRPVGKYSMAVAPYKSGRIVKYQNVSAIAYFNYIGAESNSSYLPAKLPKPDDKLAVKTVMDGLRSLNQVNVFKEIDKNIFITIGLNVKKCHSKTPKQNCQAMNNGVLTASMNNISFIHPYISILEAYYKKINGSYTEDFPDAPSKFYDFVNGAPNNIPYDTESLNGTRTKVLEYGTRVQLILQNTGTVNIENHPIHIHGYSFYVVGYGTGNYNPLTAQFNLVDPPYMNTIGVPVGGWAAVRFVADNPGVWYMHCHIDIHQSWGLGMVFIVKNGKGELESLPHPPPDLPSC