AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043474

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043474.t1 MTR_2g090900 95.181 332 8 2 1 332 1 324 0.0 634
MS.gene043474.t1 MTR_2g090900 95.455 330 7 2 1 330 1 322 0.0 631
MS.gene043474.t1 MTR_4g079500 62.428 346 95 13 1 330 1 327 4.34e-123 357
MS.gene043474.t1 MTR_2g078370 38.938 226 105 10 1 205 1 214 3.31e-29 112
MS.gene043474.t1 MTR_8g088650 35.094 265 145 8 44 294 73 324 6.19e-28 112
MS.gene043474.t1 MTR_2g078360 66.176 68 17 1 253 314 1 68 5.63e-22 89.0
MS.gene043474.t1 MTR_5g075390 48.936 94 48 0 198 291 158 251 2.68e-20 90.9
MS.gene043474.t1 MTR_4g072090 44.828 116 56 1 197 312 358 465 5.04e-20 91.3
MS.gene043474.t1 MTR_3g005230 51.724 87 42 0 208 294 136 222 7.56e-20 87.8
MS.gene043474.t1 MTR_2g099050 44.737 114 58 2 197 310 219 327 3.17e-19 88.2
MS.gene043474.t1 MTR_3g467120 46.237 93 50 0 199 291 177 269 2.10e-18 85.5
MS.gene043474.t1 MTR_6g034945 47.368 95 50 0 197 291 149 243 1.73e-17 82.0
MS.gene043474.t1 MTR_3g094020 45.745 94 51 0 198 291 107 200 3.10e-17 81.3
MS.gene043474.t1 MTR_4g072090 47.561 82 43 0 197 278 358 439 2.05e-15 77.0
MS.gene043474.t1 MTR_1g023690 48.980 98 46 2 198 293 386 481 1.98e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043474.t1 AT3G58120 58.841 345 110 10 1 330 1 328 1.88e-118 345
MS.gene043474.t1 AT2G42380 57.101 345 107 8 1 330 1 319 1.50e-113 332
MS.gene043474.t1 AT2G42380 53.623 345 108 9 1 330 1 308 9.18e-102 302
MS.gene043474.t1 AT3G58120 58.020 293 96 8 1 278 1 281 4.38e-100 296
MS.gene043474.t1 AT2G42380 56.701 291 87 7 1 278 1 265 8.80e-93 277
MS.gene043474.t1 AT2G42380 49.206 252 89 7 1 239 1 226 9.88e-62 197
MS.gene043474.t1 AT2G42380 49.206 252 89 7 1 239 1 226 9.88e-62 197
MS.gene043474.t1 AT1G58110 30.448 335 161 11 2 294 20 324 1.96e-27 110
MS.gene043474.t1 AT1G58110 30.448 335 161 11 2 294 20 324 1.96e-27 110
MS.gene043474.t1 AT1G43700 46.491 114 56 1 185 293 175 288 6.10e-21 92.4
MS.gene043474.t1 AT4G38900 48.421 95 49 0 197 291 392 486 6.34e-20 90.9
MS.gene043474.t1 AT4G38900 48.421 95 49 0 197 291 392 486 6.34e-20 90.9
MS.gene043474.t1 AT5G04840 29.766 299 158 8 2 291 5 260 2.98e-19 87.0
MS.gene043474.t1 AT2G40620 46.316 95 51 0 197 291 146 240 3.92e-19 87.4
MS.gene043474.t1 AT1G06070 46.316 95 51 0 197 291 208 302 1.49e-18 86.3
MS.gene043474.t1 AT1G06850 48.387 93 48 0 199 291 148 240 1.55e-18 85.5
MS.gene043474.t1 AT2G31370 31.174 247 143 5 62 291 57 293 4.45e-18 84.7
MS.gene043474.t1 AT2G21230 46.392 97 52 0 197 293 368 464 5.21e-18 85.1
MS.gene043474.t1 AT2G21230 46.392 97 52 0 197 293 368 464 5.21e-18 85.1
MS.gene043474.t1 AT2G31370 31.174 247 143 5 62 291 57 293 5.87e-18 84.3
MS.gene043474.t1 AT2G31370 31.174 247 143 5 62 291 57 293 5.87e-18 84.3
MS.gene043474.t1 AT2G31370 31.174 247 143 5 62 291 57 293 5.87e-18 84.3
MS.gene043474.t1 AT2G31370 31.174 247 143 5 62 291 57 293 5.87e-18 84.3
MS.gene043474.t1 AT2G31370 31.174 247 143 5 62 291 57 293 5.87e-18 84.3
MS.gene043474.t1 AT2G31370 31.174 247 143 5 62 291 57 293 5.87e-18 84.3
MS.gene043474.t1 AT4G38900 45.545 101 49 1 197 291 392 492 1.06e-17 84.3
MS.gene043474.t1 AT1G35490 28.226 248 133 7 46 291 54 258 4.87e-17 80.9
MS.gene043474.t1 AT1G35490 28.226 248 133 7 46 291 54 258 4.87e-17 80.9
MS.gene043474.t1 AT5G04840 29.371 286 150 8 2 278 5 247 4.19e-16 77.0
MS.gene043474.t1 AT2G21230 42.718 103 53 1 197 293 368 470 9.71e-16 78.6
MS.gene043474.t1 AT2G21230 46.341 82 44 0 197 278 368 449 3.74e-14 73.6
MS.gene043474.t1 AT5G07160 41.237 97 57 0 195 291 44 140 3.01e-12 64.3
MS.gene043474.t1 AT1G06850 68.889 45 14 0 198 242 147 191 5.89e-11 62.0
MS.gene043474.t1 AT2G12900 31.132 106 73 0 190 295 107 212 9.01e-11 62.0

Find 51 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGACATGTTTGGGATCTT+AGG 0.259620 2.2:+8547568 None:intergenic
GTTTGTAGTGACAATGTTAT+TGG 0.263094 2.2:+8547489 None:intergenic
GCATTAGAATCTTGGCAAAT+AGG 0.275046 2.2:-8546578 MS.gene043474:intron
GAGAAGAAGGAAAATGATTT+AGG 0.296863 2.2:-8547145 MS.gene043474:CDS
GCCAACTTGGTGACATGTTT+GGG 0.333815 2.2:+8547560 None:intergenic
CATTGGCTTTCTACCTCATC+AGG 0.351950 2.2:+8547079 None:intergenic
GCGTAGTGTAACTTCATTGC+AGG 0.377738 2.2:-8546502 MS.gene043474:intron
GGCCAACTTGGTGACATGTT+TGG 0.381627 2.2:+8547559 None:intergenic
CCATCTTCTTGAGGTTCTGA+TGG 0.385345 2.2:+8545083 None:intergenic
GACAATGTTATTGGTTGAAA+TGG 0.385822 2.2:+8547498 None:intergenic
TCTGTTTGAGAATCACATAT+TGG 0.388915 2.2:+8545019 None:intergenic
GAAGAATAAACAAGAGAAGA+AGG 0.396694 2.2:-8547158 MS.gene043474:CDS
ACCAATGCTTGATGATTGTC+GGG 0.399558 2.2:-8547356 MS.gene043474:CDS
AAGAAGATGGAAAATGCTAA+TGG 0.406779 2.2:-8545070 MS.gene043474:CDS
ATCAGAATCAGAACCCTTCA+TGG 0.433073 2.2:-8547462 MS.gene043474:CDS
GGAGATAGAAAGATTAAGAC+AGG 0.441141 2.2:-8545113 MS.gene043474:CDS
GCATTGTTGTTGTTACTTTG+AGG 0.444336 2.2:+8547037 None:intergenic
CACCAATGCTTGATGATTGT+CGG 0.462711 2.2:-8547357 MS.gene043474:CDS
CCATCAGAACCTCAAGAAGA+TGG 0.469615 2.2:-8545083 MS.gene043474:CDS
TTCTGATCTGAAGGACTCGA+AGG 0.485897 2.2:+8547208 None:intergenic
GGTAGAAAGCCAATGCAAAC+AGG 0.488493 2.2:-8547071 MS.gene043474:CDS
ATGTTTAGTGATGAAATATC+TGG 0.505923 2.2:-8547268 MS.gene043474:CDS
TGAAAAGCTTGTCTTGTGCA+AGG 0.507196 2.2:+8546218 None:intergenic
TTATTGGTTGAAATGGATGT+TGG 0.515770 2.2:+8547505 None:intergenic
TTGACAAAATTCTGATCTGA+AGG 0.516864 2.2:+8547199 None:intergenic
CCCCGACAATCATCAAGCAT+TGG 0.530465 2.2:+8547355 None:intergenic
TTTAGGAGAGATGAAGAAAG+AGG 0.538946 2.2:-8547128 MS.gene043474:CDS
AGGAACTCATCCACCCATGA+AGG 0.539506 2.2:+8547448 None:intergenic
GAAAAGCTTGTCTTGTGCAA+GGG 0.539760 2.2:+8546219 None:intergenic
CTGATCCAAAGAGAGTCAAA+AGG 0.572655 2.2:-8546991 MS.gene043474:intron
TTCGATGAAGACAACGTTGG+TGG 0.575587 2.2:+8547232 None:intergenic
TCCCAAACATGTCACCAAGT+TGG 0.580152 2.2:-8547561 MS.gene043474:CDS
AGATATTTCATCACTAAACA+TGG 0.586033 2.2:+8547270 None:intergenic
GACATGTTTGGGATCTTAGG+AGG 0.588767 2.2:+8547571 None:intergenic
CTCATCAAGAGGCATTGAAG+AGG 0.590362 2.2:-8545135 MS.gene043474:CDS
CAAGGCGAGGTGCTCATCGA+AGG 0.594155 2.2:-8547411 MS.gene043474:CDS
TCATCAAGAGGCATTGAAGA+GGG 0.599782 2.2:-8545134 MS.gene043474:CDS
GAATCAGAACCCTTCATGGG+TGG 0.607660 2.2:-8547458 MS.gene043474:CDS
ATTGAAATATGAACCTGATG+AGG 0.608223 2.2:-8547092 MS.gene043474:CDS
TCAGAATCAGAACCCTTCAT+GGG 0.609308 2.2:-8547461 MS.gene043474:CDS
TCTGATCTGAAGGACTCGAA+GGG 0.610267 2.2:+8547209 None:intergenic
TTTACATGCAGCTCATCAAG+AGG 0.610732 2.2:-8545146 MS.gene043474:intron
ATGAGCACCTCGCCTTGCCG+AGG 0.614435 2.2:+8547417 None:intergenic
GGAACTCATCCACCCATGAA+GGG 0.626852 2.2:+8547449 None:intergenic
CAAATAGGCAATCAGCACAA+AGG 0.647617 2.2:-8546563 MS.gene043474:CDS
GCGAGGTGCTCATCGAAGGT+CGG 0.657525 2.2:-8547407 MS.gene043474:CDS
CAGCACAAAGGTCACGTGTG+AGG 0.661809 2.2:-8546551 MS.gene043474:CDS
GCCTTGCCGAGGAAAAGTCG+AGG 0.688307 2.2:+8547428 None:intergenic
GATTGTCGGGGTAAACACGA+TGG 0.708511 2.2:-8547343 MS.gene043474:CDS
GGGTTCGATGAAGACAACGT+TGG 0.721008 2.2:+8547229 None:intergenic
CCAATGCTTGATGATTGTCG+GGG 0.745183 2.2:-8547355 MS.gene043474:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTATTAATAATAATTAC+TGG - chr2.2:8547155-8547174 MS.gene043474:CDS 10.0%
!! AATTAATGAAAAACAATGAT+TGG + chr2.2:8547181-8547200 None:intergenic 15.0%
!! AGAATTAATATCAATATTGA+AGG - chr2.2:8546819-8546838 MS.gene043474:intron 15.0%
!! AGATAAAAATTAAAACATGT+AGG - chr2.2:8545494-8545513 MS.gene043474:intron 15.0%
!! ATATTTGAAAAATACTTAGT+TGG + chr2.2:8547020-8547039 None:intergenic 15.0%
!! ATGAAATGACAAAATAAAAA+TGG - chr2.2:8546375-8546394 MS.gene043474:intron 15.0%
!! CTAAATAGTATTAACTATTA+AGG + chr2.2:8547213-8547232 None:intergenic 15.0%
!! TACTAATTAGTGAATTTATA+TGG - chr2.2:8546294-8546313 MS.gene043474:CDS 15.0%
!! TTTAAATCTGATAAGTAAAA+AGG + chr2.2:8547077-8547096 None:intergenic 15.0%
!!! TTGCAGAATTTTTTTTTATA+AGG - chr2.2:8547566-8547585 MS.gene043474:CDS 15.0%
!! AAATTTAATAAGAGTGAAAC+AGG - chr2.2:8546472-8546491 MS.gene043474:intron 20.0%
!! AAGTATTTGTACATTCTATT+TGG + chr2.2:8546993-8547012 None:intergenic 20.0%
!! AATTGAAATATTGTTGATCT+TGG - chr2.2:8545741-8545760 MS.gene043474:intron 20.0%
!! TATATTAACTACTTTGTGAT+GGG + chr2.2:8546588-8546607 None:intergenic 20.0%
!!! ATGTTGGCATAATATTTTTT+TGG + chr2.2:8544917-8544936 None:intergenic 20.0%
!!! CATAATATTTTTTTGGTGTT+TGG + chr2.2:8544910-8544929 None:intergenic 20.0%
! ACTAAAAAATTGTGTCATGT+GGG + chr2.2:8545635-8545654 None:intergenic 25.0%
! ACTGTCCAAATATATATTGA+AGG - chr2.2:8546097-8546116 MS.gene043474:intron 25.0%
! AGATATTTCATCACTAAACA+TGG + chr2.2:8545168-8545187 None:intergenic 25.0%
! ATATATATTTGGACAGTAGA+AGG + chr2.2:8546094-8546113 None:intergenic 25.0%
! ATATTAACTACTTTGTGATG+GGG + chr2.2:8546587-8546606 None:intergenic 25.0%
! ATGTTTAGTGATGAAATATC+TGG - chr2.2:8545167-8545186 MS.gene043474:intron 25.0%
! ATTTGCAGTACTATAATATC+TGG - chr2.2:8546003-8546022 MS.gene043474:intron 25.0%
! GTATATTAACTACTTTGTGA+TGG + chr2.2:8546589-8546608 None:intergenic 25.0%
! GTTAGTCAAAATAGTTTCTT+AGG - chr2.2:8546720-8546739 MS.gene043474:intron 25.0%
! TACTAAAAAATTGTGTCATG+TGG + chr2.2:8545636-8545655 None:intergenic 25.0%
! TTCAGCCTTCAATATATATT+TGG + chr2.2:8546105-8546124 None:intergenic 25.0%
!!! CTCTTGAAATTTTGATACAT+AGG + chr2.2:8546682-8546701 None:intergenic 25.0%
!!! GTTTTTTTGCATTAGAATCT+TGG - chr2.2:8545849-8545868 MS.gene043474:intron 25.0%
AAGAAGATGGAAAATGCTAA+TGG - chr2.2:8547365-8547384 MS.gene043474:CDS 30.0%
ACTATTTAGACACATAGCTA+GGG - chr2.2:8547224-8547243 MS.gene043474:CDS 30.0%
ATTATTGTGCTTACTCGTTA+GGG - chr2.2:8546842-8546861 MS.gene043474:intron 30.0%
ATTGAAATATGAACCTGATG+AGG - chr2.2:8545343-8545362 MS.gene043474:intron 30.0%
CAGTGCTATATATAGCTTAT+TGG + chr2.2:8546411-8546430 None:intergenic 30.0%
GAAGAATAAACAAGAGAAGA+AGG - chr2.2:8545277-8545296 MS.gene043474:intron 30.0%
GAGAAGAAGGAAAATGATTT+AGG - chr2.2:8545290-8545309 MS.gene043474:intron 30.0%
GTTTGTAGTGACAATGTTAT+TGG + chr2.2:8544949-8544968 None:intergenic 30.0%
TACTATTTAGACACATAGCT+AGG - chr2.2:8547223-8547242 MS.gene043474:CDS 30.0%
TATTAACTACTTTGTGATGG+GGG + chr2.2:8546586-8546605 None:intergenic 30.0%
TCTGTTTGAGAATCACATAT+TGG + chr2.2:8547419-8547438 None:intergenic 30.0%
TTGACAAAATTCTGATCTGA+AGG + chr2.2:8545239-8545258 None:intergenic 30.0%
! GACAATGTTATTGGTTGAAA+TGG + chr2.2:8544940-8544959 None:intergenic 30.0%
! TTATTGGTTGAAATGGATGT+TGG + chr2.2:8544933-8544952 None:intergenic 30.0%
!! AATTACCTTTTGACTCTCTT+TGG + chr2.2:8545452-8545471 None:intergenic 30.0%
!!! TTTTGGTGTTTGGAAAAATC+TGG + chr2.2:8544900-8544919 None:intergenic 30.0%
AAAAGGAGCTAACTACTTTG+AGG + chr2.2:8547060-8547079 None:intergenic 35.0%
AGTCTACTAACATTTGAGCT+CGG - chr2.2:8546615-8546634 MS.gene043474:intron 35.0%
GAATCAGTTGCAAAACTCAA+AGG + chr2.2:8545827-8545846 None:intergenic 35.0%
GATTATTGTGCTTACTCGTT+AGG - chr2.2:8546841-8546860 MS.gene043474:intron 35.0%
GCATTAGAATCTTGGCAAAT+AGG - chr2.2:8545857-8545876 MS.gene043474:intron 35.0%
GGAGATAGAAAGATTAAGAC+AGG - chr2.2:8547322-8547341 MS.gene043474:CDS 35.0%
TATGTTTGTGTGCGTTCAAA+TGG + chr2.2:8546885-8546904 None:intergenic 35.0%
TGATCCAAAAATGCAACTCT+TGG + chr2.2:8546143-8546162 None:intergenic 35.0%
TTTAGGAGAGATGAAGAAAG+AGG - chr2.2:8545307-8545326 MS.gene043474:intron 35.0%
! ATCTCCAAGAGTTGCATTTT+TGG - chr2.2:8546136-8546155 MS.gene043474:intron 35.0%
! CAAGACAAGCTTTTCAAAGA+TGG - chr2.2:8546224-8546243 MS.gene043474:CDS 35.0%
! GTGAATTTTCCTGTTTGCAT+TGG + chr2.2:8545376-8545395 None:intergenic 35.0%
! TAGCATTTTCCATCTTCTTG+AGG + chr2.2:8547364-8547383 None:intergenic 35.0%
!! ATTTCATGCTTGACTTGTCA+TGG + chr2.2:8546362-8546381 None:intergenic 35.0%
!! GCATTGTTGTTGTTACTTTG+AGG + chr2.2:8545401-8545420 None:intergenic 35.0%
!! TTGAGTTTTGCAACTGATTC+TGG - chr2.2:8545827-8545846 MS.gene043474:intron 35.0%
!!! ACATATTGGCTTTTGTGATG+GGG + chr2.2:8547405-8547424 None:intergenic 35.0%
!!! CACATATTGGCTTTTGTGAT+GGG + chr2.2:8547406-8547425 None:intergenic 35.0%
!!! TCACATATTGGCTTTTGTGA+TGG + chr2.2:8547407-8547426 None:intergenic 35.0%
ATCAGAATCAGAACCCTTCA+TGG - chr2.2:8544973-8544992 MS.gene043474:intron 40.0%
CAAATAGGCAATCAGCACAA+AGG - chr2.2:8545872-8545891 MS.gene043474:intron 40.0%
CTGATCCAAAGAGAGTCAAA+AGG - chr2.2:8545444-8545463 MS.gene043474:intron 40.0%
GAAAAGCTTGTCTTGTGCAA+GGG + chr2.2:8546219-8546238 None:intergenic 40.0%
GCACTGTAGTTACTTGAAGT+TGG - chr2.2:8546425-8546444 MS.gene043474:intron 40.0%
GGGCACATGATCACATAAAT+AGG + chr2.2:8546566-8546585 None:intergenic 40.0%
GTCTACTAACATTTGAGCTC+GGG - chr2.2:8546616-8546635 MS.gene043474:intron 40.0%
GTGAAAGAATATGTACCCTG+TGG - chr2.2:8546325-8546344 MS.gene043474:intron 40.0%
GTTGGTCAAGTATTCACTAG+TGG - chr2.2:8546443-8546462 MS.gene043474:intron 40.0%
TCAGAATCAGAACCCTTCAT+GGG - chr2.2:8544974-8544993 MS.gene043474:intron 40.0%
TGAAAAGCTTGTCTTGTGCA+AGG + chr2.2:8546220-8546239 None:intergenic 40.0%
TGTGTGCAGTCTCATAACTA+TGG + chr2.2:8546499-8546518 None:intergenic 40.0%
TTGGAAAAATCTGGCCAACT+TGG + chr2.2:8544891-8544910 None:intergenic 40.0%
TTTACATGCAGCTCATCAAG+AGG - chr2.2:8547289-8547308 MS.gene043474:CDS 40.0%
! ACCAATGCTTGATGATTGTC+GGG - chr2.2:8545079-8545098 MS.gene043474:CDS 40.0%
! CACCAATGCTTGATGATTGT+CGG - chr2.2:8545078-8545097 MS.gene043474:CDS 40.0%
! TGACTTGTCATGGTCTCATT+AGG + chr2.2:8546352-8546371 None:intergenic 40.0%
!! TCATCAAGAGGCATTGAAGA+GGG - chr2.2:8547301-8547320 MS.gene043474:CDS 40.0%
!! TTTTGTGATGGGGATGGTAA+GGG + chr2.2:8547395-8547414 None:intergenic 40.0%
ATGGTCTCATTAGGACCACA+GGG + chr2.2:8546343-8546362 None:intergenic 45.0%
CCATCAGAACCTCAAGAAGA+TGG - chr2.2:8547352-8547371 MS.gene043474:CDS 45.0%
GCGTAGTGTAACTTCATTGC+AGG - chr2.2:8545933-8545952 MS.gene043474:intron 45.0%
GGTAGAAAGCCAATGCAAAC+AGG - chr2.2:8545364-8545383 MS.gene043474:intron 45.0%
TCCCAAACATGTCACCAAGT+TGG - chr2.2:8544874-8544893 MS.gene043474:intron 45.0%
TCTGATCTGAAGGACTCGAA+GGG + chr2.2:8545229-8545248 None:intergenic 45.0%
TTCGATGAAGACAACGTTGG+TGG + chr2.2:8545206-8545225 None:intergenic 45.0%
TTCTGATCTGAAGGACTCGA+AGG + chr2.2:8545230-8545249 None:intergenic 45.0%
! CCAATGCTTGATGATTGTCG+GGG - chr2.2:8545080-8545099 MS.gene043474:CDS 45.0%
! CCATCTTCTTGAGGTTCTGA+TGG + chr2.2:8547355-8547374 None:intergenic 45.0%
! GACATGTTTGGGATCTTAGG+AGG + chr2.2:8544867-8544886 None:intergenic 45.0%
! GCCAACTTGGTGACATGTTT+GGG + chr2.2:8544878-8544897 None:intergenic 45.0%
! GGTGACATGTTTGGGATCTT+AGG + chr2.2:8544870-8544889 None:intergenic 45.0%
! TGAGTTCCTCGACTTTTCCT+CGG - chr2.2:8545001-8545020 MS.gene043474:CDS 45.0%
!! CATTGGCTTTCTACCTCATC+AGG + chr2.2:8545359-8545378 None:intergenic 45.0%
!! CTCATCAAGAGGCATTGAAG+AGG - chr2.2:8547300-8547319 MS.gene043474:CDS 45.0%
!! CTTTTGTGATGGGGATGGTA+AGG + chr2.2:8547396-8547415 None:intergenic 45.0%
!!! ATTGGCTTTTGTGATGGGGA+TGG + chr2.2:8547401-8547420 None:intergenic 45.0%
AGGAACTCATCCACCCATGA+AGG + chr2.2:8544990-8545009 None:intergenic 50.0%
CATGGTCTCATTAGGACCAC+AGG + chr2.2:8546344-8546363 None:intergenic 50.0%
CCCCGACAATCATCAAGCAT+TGG + chr2.2:8545083-8545102 None:intergenic 50.0%
GAATCAGAACCCTTCATGGG+TGG - chr2.2:8544977-8544996 MS.gene043474:intron 50.0%
GATTGTCGGGGTAAACACGA+TGG - chr2.2:8545092-8545111 MS.gene043474:CDS 50.0%
GGAACTCATCCACCCATGAA+GGG + chr2.2:8544989-8545008 None:intergenic 50.0%
GGGTTCGATGAAGACAACGT+TGG + chr2.2:8545209-8545228 None:intergenic 50.0%
! GGCCAACTTGGTGACATGTT+TGG + chr2.2:8544879-8544898 None:intergenic 50.0%
!! GGAGCTAACTACTTTGAGGC+AGG + chr2.2:8547056-8547075 None:intergenic 50.0%
CAGCACAAAGGTCACGTGTG+AGG - chr2.2:8545884-8545903 MS.gene043474:intron 55.0%
! TCCTCGACTTTTCCTCGGCA+AGG - chr2.2:8545006-8545025 MS.gene043474:CDS 55.0%
GCCTTGCCGAGGAAAAGTCG+AGG + chr2.2:8545010-8545029 None:intergenic 60.0%
! CAAGGCGAGGTGCTCATCGA+AGG - chr2.2:8545024-8545043 MS.gene043474:CDS 60.0%
! GACTTTTCCTCGGCAAGGCG+AGG - chr2.2:8545011-8545030 MS.gene043474:CDS 60.0%
! GCGAGGTGCTCATCGAAGGT+CGG - chr2.2:8545028-8545047 MS.gene043474:CDS 60.0%
ATGAGCACCTCGCCTTGCCG+AGG + chr2.2:8545021-8545040 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 8544852 8547605 8544852 ID=MS.gene043474
chr2.2 mRNA 8544852 8547605 8544852 ID=MS.gene043474.t1;Parent=MS.gene043474
chr2.2 exon 8546992 8547605 8546992 ID=MS.gene043474.t1.exon1;Parent=MS.gene043474.t1
chr2.2 CDS 8546992 8547605 8546992 ID=cds.MS.gene043474.t1;Parent=MS.gene043474.t1
chr2.2 exon 8546503 8546593 8546503 ID=MS.gene043474.t1.exon2;Parent=MS.gene043474.t1
chr2.2 CDS 8546503 8546593 8546503 ID=cds.MS.gene043474.t1;Parent=MS.gene043474.t1
chr2.2 exon 8546212 8546338 8546212 ID=MS.gene043474.t1.exon3;Parent=MS.gene043474.t1
chr2.2 CDS 8546212 8546338 8546212 ID=cds.MS.gene043474.t1;Parent=MS.gene043474.t1
chr2.2 exon 8545000 8545157 8545000 ID=MS.gene043474.t1.exon4;Parent=MS.gene043474.t1
chr2.2 CDS 8545000 8545157 8545000 ID=cds.MS.gene043474.t1;Parent=MS.gene043474.t1
chr2.2 exon 8544852 8544869 8544852 ID=MS.gene043474.t1.exon5;Parent=MS.gene043474.t1
chr2.2 CDS 8544852 8544869 8544852 ID=cds.MS.gene043474.t1;Parent=MS.gene043474.t1
Gene Sequence

>MS.gene043474

ATGGCACAATTACCTCCTAAGATCCCAAACATGTCACCAAGTTGGCCAGATTTTTCCAAACACCAAAAAAATATTATGCCAACATCCATTTCAACCAATAACATTGTCACTACAAACAATTATCAGAATCAGAACCCTTCATGGGTGGATGAGTTCCTCGACTTTTCCTCGGCAAGGCGAGGTGCTCATCGAAGGTCGGTCAGCGATTCAGTCACATTCTTAGAAACACCAATGCTTGATGATTGTCGGGGTAAACACGATGGTAACAACAATGACAACGAGTTCGACAAGTTTGATGATGAACAATTTTTGTCCATGTTTAGTGATGAAATATCTGGTGTCAATAACATGCCACCAACGTTGTCTTCATCGAACCCTTCGAGTCCTTCAGATCAGAATTTTGTCAATGATGATAATGAGAAAGAGAAGAATAAACAAGAGAAGAAGGAAAATGATTTAGGAGAGATGAAGAAAGAGGAAGATGAGAAGCAATTGAAATATGAACCTGATGAGGTAGAAAGCCAATGCAAACAGGAAAATTCACAGCCTCAAAGTAACAACAACAATGCAACTTCTTCAAATGATAGAATTACTGATCCAAAGAGAGTCAAAAGGTAATTATCAAACACTTCTATATACTATAGATAAAAATTAAAACATGTAGGATGAAATTTAAAACAATTTTTTTTTTCTTCTAATTTTCTTGCATAATTCAACTTTTTCTAACAGTATATTTTTATCTATATACATATGCTTAATTTTGCTAATAAACTTGCAAAGCCCACATGACACAATTTTTTAGTACTTCATTATTGTAGATTATTTTTTTAAGCATGATTTTGATATTCTAGTTGAAATTGTTGATTAGTTTTCATGCATTGCTCTTTATAATTGAAATATTGTTGATCTTGGTTTCTTGAACTTTTTATATATTTTTTTCTTTGTTTGTAAATGAAAATAAAGCATTTTTTTCCTTTGAGTTTTGCAACTGATTCTGGTTTTTTTGCATTAGAATCTTGGCAAATAGGCAATCAGCACAAAGGTCACGTGTGAGGAAGCTGCAATACATATCAGAGCTTGAGCGTAGTGTAACTTCATTGCAGGTTTGTTACGTAACATAACATGATTTCACTATAATTTTTGTATTATTTATTTGCAGTACTATAATATCTGGAAATTTAATGTATTATGTATATAGTAGAAATGATCAAATAATAATGTTATTGACTTTTTGTTATTCCTTCTACTGTCCAAATATATATTGAAGGCTGAAGTTTCAGTGCTATCTCCAAGAGTTGCATTTTTGGATCATCAACGATTGCTTCTAAATGTTGACAACAGTGCTCTCAAGCAAAGAATTGCAGCCCTTGCACAAGACAAGCTTTTCAAAGATGGTAATTTAATTATTTCTTCAATACTATATTGTTCAATTTAATTATTATTACTAATTAGTGAATTTATATGGATATATGAGTGAAAGAATATGTACCCTGTGGTCCTAATGAGACCATGACAAGTCAAGCATGAAATGACAAAATAAAAATGGAGAAAACACACCAATAAGCTATATATAGCACTGTAGTTACTTGAAGTTGGTCAAGTATTCACTAGTGGTAAATTAAATTTAATAAGAGTGAAACAGGTCCATAGTTATGAGACTGCACACAGAGTTATAATGAATTTAGTGAATGTGTTTTTGTTTGCATGCACGCCTATTTATGTGATCATGTGCCCCCATCACAAAGTAGTTAATATACTCAAAAAGTCTACTAACATTTGAGCTCGGGTAATAGTCATTTTTGTCTTTGAATATGTAACAAATATAGTCCTATGTATCAAAATTTCAAGAGTTTCTAATTGTACAATATGTTAGTCAAAATAGTTTCTTAGGTTAAAATGTCTCGTTAATATTGTAATAATAGTTCTTCAATATACTTATTTATTGTCATATCAATCTCTATATAAAAAGAATTAATATCAATATTGAAGGATTATTGTGCTTACTCGTTAGGGACTAAAATGACTATACTCCATTTGAACGCACACAAACATAAAAATACTATCTTTTAATAATTTTAAAAATATAGATTATTTTATTTTATTTCTTTTTTCATCTTTTATTAAGTGTGTGAATGTGTCCAAATAGAATGTACAAATACTTGTGACCAACTAAGTATTTTTCAAATATACTTGTCTAGTGTCCTGCCTCAAAGTAGTTAGCTCCTTTTTACTTATCAGATTTAAAGTTGTTTTGTACTGTTAGTAGTTGTTATTTTTTAAAGTTTAATGTAACATTAGTTATTGTTTTATTAATAATAATTACTGGCCAATCATTGTTTTTCATTAATTATTGAATATCCTTAATAGTTAATACTATTTAGACACATAGCTAGGGTGAAGCTTCAGATATTGTACTTACTATAATGACATTTATATATTTACATGCAGCTCATCAAGAGGCATTGAAGAGGGAGATAGAAAGATTAAGACAGGTATATCTCCATCAGAACCTCAAGAAGATGGAAAATGCTAATGGATCACCCTTACCATCCCCATCACAAAAGCCAATATGTGATTCTCAAACAGAAAGCGAACAACTACTCAATGTATGAACTCAACTCTTCAGAAAACTCATACTAATTAAGTAGCTAGCTAGTTTATATATTATTCTGTTACATATTACTGTGAATCTTTTATTTTATAAAAAAAAGACATTGCAGAATTTTTTTTTATAAGGAAAATGAGAGATTTTAA
Protein sequence

>MS.gene043474.t1

MAQLPPKIPNMSPSWPDFSKHQKNIMPTSISTNNIVTTNNYQNQNPSWVDEFLDFSSARRGAHRRSVSDSVTFLETPMLDDCRGKHDGNNNDNEFDKFDDEQFLSMFSDEISGVNNMPPTLSSSNPSSPSDQNFVNDDNEKEKNKQEKKENDLGEMKKEEDEKQLKYEPDEVESQCKQENSQPQSNNNNATSSNDRITDPKRVKRILANRQSAQRSRVRKLQYISELERSVTSLQAEVSVLSPRVAFLDHQRLLLNVDNSALKQRIAALAQDKLFKDAHQEALKREIERLRQVYLHQNLKKMENANGSPLPSPSQKPICDSQTESEQLLNENERF