AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31409

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31409.t1 MTR_2g090900 95.758 330 6 2 1 330 1 322 0.0 638
MS.gene31409.t1 MTR_2g090900 95.770 331 6 2 1 331 1 323 0.0 638
MS.gene31409.t1 MTR_4g079500 62.931 348 92 14 1 331 1 328 6.52e-125 361
MS.gene31409.t1 MTR_2g078370 38.938 226 105 10 1 205 1 214 3.29e-29 112
MS.gene31409.t1 MTR_8g088650 35.094 265 145 8 44 294 73 324 3.82e-28 112
MS.gene31409.t1 MTR_2g078360 67.647 68 16 1 253 314 1 68 1.49e-23 93.2
MS.gene31409.t1 MTR_5g075390 48.936 94 48 0 198 291 158 251 2.50e-20 90.9
MS.gene31409.t1 MTR_4g072090 44.828 116 56 1 197 312 358 465 3.78e-20 91.3
MS.gene31409.t1 MTR_3g005230 51.724 87 42 0 208 294 136 222 6.34e-20 87.8
MS.gene31409.t1 MTR_2g099050 44.737 114 58 2 197 310 219 327 3.31e-19 88.2
MS.gene31409.t1 MTR_3g467120 46.237 93 50 0 199 291 177 269 1.82e-18 85.5
MS.gene31409.t1 MTR_6g034945 46.000 100 54 0 197 296 149 248 6.53e-18 83.2
MS.gene31409.t1 MTR_3g094020 45.745 94 51 0 198 291 107 200 2.25e-17 81.6
MS.gene31409.t1 MTR_4g072090 47.561 82 43 0 197 278 358 439 1.89e-15 77.0
MS.gene31409.t1 MTR_1g023690 48.980 98 46 2 198 293 386 481 1.64e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31409.t1 AT3G58120 59.249 346 109 10 1 331 1 329 8.07e-121 351
MS.gene31409.t1 AT2G42380 57.225 346 107 8 1 331 1 320 7.80e-115 335
MS.gene31409.t1 AT2G42380 53.757 346 108 9 1 331 1 309 2.66e-103 305
MS.gene31409.t1 AT3G58120 58.020 293 96 8 1 278 1 281 6.63e-100 296
MS.gene31409.t1 AT2G42380 56.701 291 87 7 1 278 1 265 8.18e-93 277
MS.gene31409.t1 AT2G42380 49.206 252 89 7 1 239 1 226 9.33e-62 197
MS.gene31409.t1 AT2G42380 49.206 252 89 7 1 239 1 226 9.33e-62 197
MS.gene31409.t1 AT1G58110 30.448 335 161 11 2 294 20 324 1.77e-27 110
MS.gene31409.t1 AT1G58110 30.448 335 161 11 2 294 20 324 1.77e-27 110
MS.gene31409.t1 AT1G43700 46.491 114 56 1 185 293 175 288 5.35e-21 92.4
MS.gene31409.t1 AT4G38900 48.421 95 49 0 197 291 392 486 5.41e-20 91.3
MS.gene31409.t1 AT4G38900 48.421 95 49 0 197 291 392 486 5.41e-20 91.3
MS.gene31409.t1 AT5G04840 29.276 304 163 8 2 296 5 265 2.14e-19 87.4
MS.gene31409.t1 AT2G40620 46.316 95 51 0 197 291 146 240 3.26e-19 87.8
MS.gene31409.t1 AT1G06850 48.387 93 48 0 199 291 148 240 1.10e-18 85.9
MS.gene31409.t1 AT1G06070 46.316 95 51 0 197 291 208 302 1.33e-18 86.7
MS.gene31409.t1 AT2G31370 31.174 247 143 5 62 291 57 293 4.22e-18 84.7
MS.gene31409.t1 AT2G21230 46.392 97 52 0 197 293 368 464 4.52e-18 85.5
MS.gene31409.t1 AT2G21230 46.392 97 52 0 197 293 368 464 4.52e-18 85.5
MS.gene31409.t1 AT2G31370 31.174 247 143 5 62 291 57 293 4.68e-18 84.7
MS.gene31409.t1 AT2G31370 31.174 247 143 5 62 291 57 293 4.68e-18 84.7
MS.gene31409.t1 AT2G31370 31.174 247 143 5 62 291 57 293 4.68e-18 84.7
MS.gene31409.t1 AT2G31370 31.174 247 143 5 62 291 57 293 4.68e-18 84.7
MS.gene31409.t1 AT2G31370 31.174 247 143 5 62 291 57 293 4.68e-18 84.7
MS.gene31409.t1 AT2G31370 31.174 247 143 5 62 291 57 293 4.68e-18 84.7
MS.gene31409.t1 AT4G38900 45.545 101 49 1 197 291 392 492 8.28e-18 84.7
MS.gene31409.t1 AT1G35490 28.226 248 133 7 46 291 54 258 5.21e-17 80.5
MS.gene31409.t1 AT1G35490 28.226 248 133 7 46 291 54 258 5.21e-17 80.5
MS.gene31409.t1 AT5G04840 29.371 286 150 8 2 278 5 247 4.14e-16 77.0
MS.gene31409.t1 AT2G21230 42.718 103 53 1 197 293 368 470 1.00e-15 78.2
MS.gene31409.t1 AT2G21230 46.341 82 44 0 197 278 368 449 3.26e-14 73.6
MS.gene31409.t1 AT5G07160 41.237 97 57 0 195 291 44 140 2.93e-12 64.3
MS.gene31409.t1 AT1G06850 68.889 45 14 0 198 242 147 191 4.97e-11 62.4

Find 51 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGACATGTTTGGGATCTT+AGG 0.259620 2.4:+9604266 None:intergenic
GTTTGTAGTGACAATGTTAT+TGG 0.263094 2.4:+9604187 None:intergenic
GCATTAGAATCTTGGCAAAT+AGG 0.275046 2.4:-9603276 MS.gene31409:intron
GAGAAGAAGGAAAATGATTT+AGG 0.296863 2.4:-9603843 MS.gene31409:CDS
GCCAACTTGGTGACATGTTT+GGG 0.333815 2.4:+9604258 None:intergenic
CATTGGCTTTCTACCTCATC+AGG 0.351950 2.4:+9603777 None:intergenic
GCGTAGTGTAACTTCATTGC+AGG 0.377738 2.4:-9603200 MS.gene31409:intron
GGCCAACTTGGTGACATGTT+TGG 0.381627 2.4:+9604257 None:intergenic
GACAATGTTATTGGTTGAAA+TGG 0.385822 2.4:+9604196 None:intergenic
TCTGTTTGAGAATCACATAT+TGG 0.388915 2.4:+9601717 None:intergenic
CCATCTTCTTGAGGTTCTGA+TGG 0.391631 2.4:+9601781 None:intergenic
GAAGAATAAACAAGAGAAGA+AGG 0.396694 2.4:-9603856 MS.gene31409:CDS
ACCAATGCTTGATGATTGTC+GGG 0.399558 2.4:-9604054 MS.gene31409:CDS
AAGAAGATGGAAAATGCTAA+TGG 0.406779 2.4:-9601768 MS.gene31409:CDS
ATCAGAATCAGAACCCTTCA+TGG 0.433073 2.4:-9604160 MS.gene31409:CDS
GGAGATAGAAAGATTAAGAC+AGG 0.441141 2.4:-9601811 MS.gene31409:CDS
GCATTGTTGTTGTTACTTTG+AGG 0.444336 2.4:+9603735 None:intergenic
CACCAATGCTTGATGATTGT+CGG 0.462711 2.4:-9604055 MS.gene31409:CDS
TTCTGATCTGAAGGACTCGA+AGG 0.485897 2.4:+9603906 None:intergenic
GGTAGAAAGCCAATGCAAAC+AGG 0.488493 2.4:-9603769 MS.gene31409:CDS
CCATCAGAACCTCAAGAAGA+TGG 0.490491 2.4:-9601781 MS.gene31409:CDS
ATGTTTAGTGATGAAATATC+TGG 0.505923 2.4:-9603966 MS.gene31409:CDS
TGAAAAGCTTGTCTTGTGCA+AGG 0.507196 2.4:+9602916 None:intergenic
TTATTGGTTGAAATGGATGT+TGG 0.515770 2.4:+9604203 None:intergenic
TTGACAAAATTCTGATCTGA+AGG 0.516864 2.4:+9603897 None:intergenic
CCCCGACAATCATCAAGCAT+TGG 0.530465 2.4:+9604053 None:intergenic
TTTAGGAGAGATGAAGAAAG+AGG 0.538946 2.4:-9603826 MS.gene31409:CDS
AGGAACTCATCCACCCATGA+AGG 0.539506 2.4:+9604146 None:intergenic
GAAAAGCTTGTCTTGTGCAA+GGG 0.539760 2.4:+9602917 None:intergenic
CTGATCCAAAGAGAGTCAAA+AGG 0.572655 2.4:-9603689 MS.gene31409:intron
TTCGATGAAGACAACGTTGG+TGG 0.575587 2.4:+9603930 None:intergenic
TCCCAAACATGTCACCAAGT+TGG 0.580152 2.4:-9604259 MS.gene31409:CDS
AGATATTTCATCACTAAACA+TGG 0.586033 2.4:+9603968 None:intergenic
GACATGTTTGGGATCTTAGG+AGG 0.588767 2.4:+9604269 None:intergenic
CTCATCAAGAGGCATTGAAG+AGG 0.590362 2.4:-9601833 MS.gene31409:CDS
CAAGGCGAGGTGCTCATCGA+AGG 0.594155 2.4:-9604109 MS.gene31409:CDS
TCATCAAGAGGCATTGAAGA+GGG 0.599782 2.4:-9601832 MS.gene31409:CDS
GAATCAGAACCCTTCATGGG+TGG 0.607660 2.4:-9604156 MS.gene31409:CDS
ATTGAAATATGAACCTGATG+AGG 0.608223 2.4:-9603790 MS.gene31409:CDS
TCAGAATCAGAACCCTTCAT+GGG 0.609308 2.4:-9604159 MS.gene31409:CDS
TCTGATCTGAAGGACTCGAA+GGG 0.610267 2.4:+9603907 None:intergenic
TTTACATGCAGCTCATCAAG+AGG 0.610732 2.4:-9601844 MS.gene31409:intron
ATGAGCACCTCGCCTTGCCG+AGG 0.614435 2.4:+9604115 None:intergenic
GGAACTCATCCACCCATGAA+GGG 0.626852 2.4:+9604147 None:intergenic
CAAATAGGCAATCAGCACAA+AGG 0.647617 2.4:-9603261 MS.gene31409:CDS
GCGAGGTGCTCATCGAAGGT+CGG 0.657525 2.4:-9604105 MS.gene31409:CDS
CAGCACAAAGGTCACGTGTG+AGG 0.661809 2.4:-9603249 MS.gene31409:CDS
GCCTTGCCGAGGAAAAGTCG+AGG 0.688307 2.4:+9604126 None:intergenic
GATTGTCGGGGTAAACACGA+TGG 0.708511 2.4:-9604041 MS.gene31409:CDS
GGGTTCGATGAAGACAACGT+TGG 0.721008 2.4:+9603927 None:intergenic
CCAATGCTTGATGATTGTCG+GGG 0.745183 2.4:-9604053 MS.gene31409:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTATTAATAATAATTAC+TGG - chr2.4:9603995-9604014 MS.gene31409:CDS 10.0%
!! AATTAATGAAAAACAATGAT+TGG + chr2.4:9604021-9604040 None:intergenic 15.0%
!! AGAATTAATATCAATATTGA+AGG - chr2.4:9603659-9603678 MS.gene31409:intron 15.0%
!! AGATAAAAATTAAAACATGT+AGG - chr2.4:9602334-9602353 MS.gene31409:intron 15.0%
!! ATATTTGAAAAATACTTAGT+TGG + chr2.4:9603860-9603879 None:intergenic 15.0%
!! ATGAAATGACAAAATAAAAA+TGG - chr2.4:9603215-9603234 MS.gene31409:CDS 15.0%
!! CTAAATAGTATTAACTATTA+AGG + chr2.4:9604053-9604072 None:intergenic 15.0%
!! TACTAATTAGTGAATTTATA+TGG - chr2.4:9603134-9603153 MS.gene31409:intron 15.0%
!! TTTAAATCTGATAAGTAAAA+AGG + chr2.4:9603917-9603936 None:intergenic 15.0%
!! AAATTTAATAAGAGTGAAAC+AGG - chr2.4:9603312-9603331 MS.gene31409:intron 20.0%
!! AAGTATTTGTACATTCTATT+TGG + chr2.4:9603833-9603852 None:intergenic 20.0%
!! AATTGAAATATTGTTGATCT+TGG - chr2.4:9602581-9602600 MS.gene31409:intron 20.0%
!! TATATTAACTACTTTGTGAT+GGG + chr2.4:9603428-9603447 None:intergenic 20.0%
!!! ATGTTGGCATAATATTTTTT+TGG + chr2.4:9601757-9601776 None:intergenic 20.0%
!!! CATAATATTTTTTTGGTGTT+TGG + chr2.4:9601750-9601769 None:intergenic 20.0%
! ACTAAAAAATTGTGTCATGT+GGG + chr2.4:9602475-9602494 None:intergenic 25.0%
! ACTGTCCAAATATATATTGA+AGG - chr2.4:9602937-9602956 MS.gene31409:CDS 25.0%
! AGATATTTCATCACTAAACA+TGG + chr2.4:9602008-9602027 None:intergenic 25.0%
! ATATATATTTGGACAGTAGA+AGG + chr2.4:9602934-9602953 None:intergenic 25.0%
! ATATTAACTACTTTGTGATG+GGG + chr2.4:9603427-9603446 None:intergenic 25.0%
! ATGTTTAGTGATGAAATATC+TGG - chr2.4:9602007-9602026 MS.gene31409:intron 25.0%
! ATTTGCAGTACTATAATATC+TGG - chr2.4:9602843-9602862 MS.gene31409:intron 25.0%
! GTATATTAACTACTTTGTGA+TGG + chr2.4:9603429-9603448 None:intergenic 25.0%
! GTTAGTCAAAATAGTTTCTT+AGG - chr2.4:9603560-9603579 MS.gene31409:intron 25.0%
! TACTAAAAAATTGTGTCATG+TGG + chr2.4:9602476-9602495 None:intergenic 25.0%
! TTCAGCCTTCAATATATATT+TGG + chr2.4:9602945-9602964 None:intergenic 25.0%
!!! CTCTTGAAATTTTGATACAT+AGG + chr2.4:9603522-9603541 None:intergenic 25.0%
!!! GTTTTTTTGCATTAGAATCT+TGG - chr2.4:9602689-9602708 MS.gene31409:intron 25.0%
AAGAAGATGGAAAATGCTAA+TGG - chr2.4:9604205-9604224 MS.gene31409:CDS 30.0%
ACTATTTAGACACATAGCTA+GGG - chr2.4:9604064-9604083 MS.gene31409:CDS 30.0%
ATTATTGTGCTTACTCGTTA+GGG - chr2.4:9603682-9603701 MS.gene31409:intron 30.0%
ATTGAAATATGAACCTGATG+AGG - chr2.4:9602183-9602202 MS.gene31409:intron 30.0%
CAGTGCTATATATAGCTTAT+TGG + chr2.4:9603251-9603270 None:intergenic 30.0%
GAAGAATAAACAAGAGAAGA+AGG - chr2.4:9602117-9602136 MS.gene31409:intron 30.0%
GAGAAGAAGGAAAATGATTT+AGG - chr2.4:9602130-9602149 MS.gene31409:intron 30.0%
GTTTGTAGTGACAATGTTAT+TGG + chr2.4:9601789-9601808 None:intergenic 30.0%
TACTATTTAGACACATAGCT+AGG - chr2.4:9604063-9604082 MS.gene31409:CDS 30.0%
TATTAACTACTTTGTGATGG+GGG + chr2.4:9603426-9603445 None:intergenic 30.0%
TCTGTTTGAGAATCACATAT+TGG + chr2.4:9604259-9604278 None:intergenic 30.0%
TTGACAAAATTCTGATCTGA+AGG + chr2.4:9602079-9602098 None:intergenic 30.0%
! GACAATGTTATTGGTTGAAA+TGG + chr2.4:9601780-9601799 None:intergenic 30.0%
! TTATTGGTTGAAATGGATGT+TGG + chr2.4:9601773-9601792 None:intergenic 30.0%
!! AATTACCTTTTGACTCTCTT+TGG + chr2.4:9602292-9602311 None:intergenic 30.0%
!!! TTTTGGTGTTTGGAAAAATC+TGG + chr2.4:9601740-9601759 None:intergenic 30.0%
AAAAGGAGCTAACTACTTTG+AGG + chr2.4:9603900-9603919 None:intergenic 35.0%
AGTCTACTAACATTTGAGCT+CGG - chr2.4:9603455-9603474 MS.gene31409:intron 35.0%
GAATCAGTTGCAAAACTCAA+AGG + chr2.4:9602667-9602686 None:intergenic 35.0%
GATTATTGTGCTTACTCGTT+AGG - chr2.4:9603681-9603700 MS.gene31409:intron 35.0%
GCATTAGAATCTTGGCAAAT+AGG - chr2.4:9602697-9602716 MS.gene31409:intron 35.0%
GGAGATAGAAAGATTAAGAC+AGG - chr2.4:9604162-9604181 MS.gene31409:CDS 35.0%
TATGTTTGTGTGCGTTCAAA+TGG + chr2.4:9603725-9603744 None:intergenic 35.0%
TGATCCAAAAATGCAACTCT+TGG + chr2.4:9602983-9603002 None:intergenic 35.0%
TTTAGGAGAGATGAAGAAAG+AGG - chr2.4:9602147-9602166 MS.gene31409:intron 35.0%
! ATCTCCAAGAGTTGCATTTT+TGG - chr2.4:9602976-9602995 MS.gene31409:CDS 35.0%
! CAAGACAAGCTTTTCAAAGA+TGG - chr2.4:9603064-9603083 MS.gene31409:intron 35.0%
! GTGAATTTTCCTGTTTGCAT+TGG + chr2.4:9602216-9602235 None:intergenic 35.0%
! TAGCATTTTCCATCTTCTTG+AGG + chr2.4:9604204-9604223 None:intergenic 35.0%
!! ATTTCATGCTTGACTTGTCA+TGG + chr2.4:9603202-9603221 None:intergenic 35.0%
!! GCATTGTTGTTGTTACTTTG+AGG + chr2.4:9602241-9602260 None:intergenic 35.0%
!! TTGAGTTTTGCAACTGATTC+TGG - chr2.4:9602667-9602686 MS.gene31409:intron 35.0%
!!! ACATATTGGCTTTTGTGATG+GGG + chr2.4:9604245-9604264 None:intergenic 35.0%
!!! CACATATTGGCTTTTGTGAT+GGG + chr2.4:9604246-9604265 None:intergenic 35.0%
!!! TCACATATTGGCTTTTGTGA+TGG + chr2.4:9604247-9604266 None:intergenic 35.0%
ATCAGAATCAGAACCCTTCA+TGG - chr2.4:9601813-9601832 MS.gene31409:CDS 40.0%
CAAATAGGCAATCAGCACAA+AGG - chr2.4:9602712-9602731 MS.gene31409:intron 40.0%
CTGATCCAAAGAGAGTCAAA+AGG - chr2.4:9602284-9602303 MS.gene31409:intron 40.0%
GAAAAGCTTGTCTTGTGCAA+GGG + chr2.4:9603059-9603078 None:intergenic 40.0%
GCACTGTAGTTACTTGAAGT+TGG - chr2.4:9603265-9603284 MS.gene31409:CDS 40.0%
GGGCACATGATCACATAAAT+AGG + chr2.4:9603406-9603425 None:intergenic 40.0%
GTCTACTAACATTTGAGCTC+GGG - chr2.4:9603456-9603475 MS.gene31409:intron 40.0%
GTGAAAGAATATGTACCCTG+TGG - chr2.4:9603165-9603184 MS.gene31409:intron 40.0%
TCAGAATCAGAACCCTTCAT+GGG - chr2.4:9601814-9601833 MS.gene31409:CDS 40.0%
TGAAAAGCTTGTCTTGTGCA+AGG + chr2.4:9603060-9603079 None:intergenic 40.0%
TGTGTGCAGTCTCATAACTA+TGG + chr2.4:9603339-9603358 None:intergenic 40.0%
TTGGAAAAATCTGGCCAACT+TGG + chr2.4:9601731-9601750 None:intergenic 40.0%
TTTACATGCAGCTCATCAAG+AGG - chr2.4:9604129-9604148 MS.gene31409:CDS 40.0%
! ACCAATGCTTGATGATTGTC+GGG - chr2.4:9601919-9601938 MS.gene31409:intron 40.0%
! CACCAATGCTTGATGATTGT+CGG - chr2.4:9601918-9601937 MS.gene31409:intron 40.0%
! TGACTTGTCATGGTCTCATT+AGG + chr2.4:9603192-9603211 None:intergenic 40.0%
!! TCATCAAGAGGCATTGAAGA+GGG - chr2.4:9604141-9604160 MS.gene31409:CDS 40.0%
!! TTTTGTGATGGGGATGGTAA+GGG + chr2.4:9604235-9604254 None:intergenic 40.0%
ATGGTCTCATTAGGACCACA+GGG + chr2.4:9603183-9603202 None:intergenic 45.0%
CCATCAGAACCTCAAGAAGA+TGG - chr2.4:9604192-9604211 MS.gene31409:CDS 45.0%
GCGTAGTGTAACTTCATTGC+AGG - chr2.4:9602773-9602792 MS.gene31409:intron 45.0%
GGTAGAAAGCCAATGCAAAC+AGG - chr2.4:9602204-9602223 MS.gene31409:intron 45.0%
TCCCAAACATGTCACCAAGT+TGG - chr2.4:9601714-9601733 MS.gene31409:CDS 45.0%
TCTGATCTGAAGGACTCGAA+GGG + chr2.4:9602069-9602088 None:intergenic 45.0%
TTCGATGAAGACAACGTTGG+TGG + chr2.4:9602046-9602065 None:intergenic 45.0%
TTCTGATCTGAAGGACTCGA+AGG + chr2.4:9602070-9602089 None:intergenic 45.0%
! CCAATGCTTGATGATTGTCG+GGG - chr2.4:9601920-9601939 MS.gene31409:intron 45.0%
! CCATCTTCTTGAGGTTCTGA+TGG + chr2.4:9604195-9604214 None:intergenic 45.0%
! GACATGTTTGGGATCTTAGG+AGG + chr2.4:9601707-9601726 None:intergenic 45.0%
! GCCAACTTGGTGACATGTTT+GGG + chr2.4:9601718-9601737 None:intergenic 45.0%
! GGTGACATGTTTGGGATCTT+AGG + chr2.4:9601710-9601729 None:intergenic 45.0%
! TGAGTTCCTCGACTTTTCCT+CGG - chr2.4:9601841-9601860 MS.gene31409:intron 45.0%
!! CATTGGCTTTCTACCTCATC+AGG + chr2.4:9602199-9602218 None:intergenic 45.0%
!! CTCATCAAGAGGCATTGAAG+AGG - chr2.4:9604140-9604159 MS.gene31409:CDS 45.0%
!! CTTTTGTGATGGGGATGGTA+AGG + chr2.4:9604236-9604255 None:intergenic 45.0%
!!! ATTGGCTTTTGTGATGGGGA+TGG + chr2.4:9604241-9604260 None:intergenic 45.0%
AGGAACTCATCCACCCATGA+AGG + chr2.4:9601830-9601849 None:intergenic 50.0%
CATGGTCTCATTAGGACCAC+AGG + chr2.4:9603184-9603203 None:intergenic 50.0%
CCCCGACAATCATCAAGCAT+TGG + chr2.4:9601923-9601942 None:intergenic 50.0%
GAATCAGAACCCTTCATGGG+TGG - chr2.4:9601817-9601836 MS.gene31409:CDS 50.0%
GATTGTCGGGGTAAACACGA+TGG - chr2.4:9601932-9601951 MS.gene31409:intron 50.0%
GGAACTCATCCACCCATGAA+GGG + chr2.4:9601829-9601848 None:intergenic 50.0%
GGGTTCGATGAAGACAACGT+TGG + chr2.4:9602049-9602068 None:intergenic 50.0%
! GGCCAACTTGGTGACATGTT+TGG + chr2.4:9601719-9601738 None:intergenic 50.0%
!! GGAGCTAACTACTTTGAGGC+AGG + chr2.4:9603896-9603915 None:intergenic 50.0%
CAGCACAAAGGTCACGTGTG+AGG - chr2.4:9602724-9602743 MS.gene31409:intron 55.0%
! TCCTCGACTTTTCCTCGGCA+AGG - chr2.4:9601846-9601865 MS.gene31409:intron 55.0%
GCCTTGCCGAGGAAAAGTCG+AGG + chr2.4:9601850-9601869 None:intergenic 60.0%
! CAAGGCGAGGTGCTCATCGA+AGG - chr2.4:9601864-9601883 MS.gene31409:intron 60.0%
! GACTTTTCCTCGGCAAGGCG+AGG - chr2.4:9601851-9601870 MS.gene31409:intron 60.0%
! GCGAGGTGCTCATCGAAGGT+CGG - chr2.4:9601868-9601887 MS.gene31409:intron 60.0%
ATGAGCACCTCGCCTTGCCG+AGG + chr2.4:9601861-9601880 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 9601692 9604303 9601692 ID=MS.gene31409
chr2.4 mRNA 9601692 9604303 9601692 ID=MS.gene31409.t1;Parent=MS.gene31409
chr2.4 exon 9603690 9604303 9603690 ID=MS.gene31409.t1.exon1;Parent=MS.gene31409.t1
chr2.4 CDS 9603690 9604303 9603690 ID=cds.MS.gene31409.t1;Parent=MS.gene31409.t1
chr2.4 exon 9603201 9603291 9603201 ID=MS.gene31409.t1.exon2;Parent=MS.gene31409.t1
chr2.4 CDS 9603201 9603291 9603201 ID=cds.MS.gene31409.t1;Parent=MS.gene31409.t1
chr2.4 exon 9602910 9603036 9602910 ID=MS.gene31409.t1.exon3;Parent=MS.gene31409.t1
chr2.4 CDS 9602910 9603036 9602910 ID=cds.MS.gene31409.t1;Parent=MS.gene31409.t1
chr2.4 exon 9601692 9601855 9601692 ID=MS.gene31409.t1.exon4;Parent=MS.gene31409.t1
chr2.4 CDS 9601692 9601855 9601692 ID=cds.MS.gene31409.t1;Parent=MS.gene31409.t1
Gene Sequence

>MS.gene31409

ATGGCACAATTACCTCCTAAGATCCCAAACATGTCACCAAGTTGGCCAGATTTTTCCAAACACCAAAAAAATATTATGCCAACATCCATTTCAACCAATAACATTGTCACTACAAACAATTATCAGAATCAGAACCCTTCATGGGTGGATGAGTTCCTCGACTTTTCCTCGGCAAGGCGAGGTGCTCATCGAAGGTCGGTCAGCGATTCAGTCACATTCTTAGAAACACCAATGCTTGATGATTGTCGGGGTAAACACGATGGTAACAACAATGACAACGAGTTCGACAAGTTTGATGATGAACAATTTTTGTCCATGTTTAGTGATGAAATATCTGGTGTCAATAACATGCCACCAACGTTGTCTTCATCGAACCCTTCGAGTCCTTCAGATCAGAATTTTGTCAATGATGATAATGAGAAAGAGAAGAATAAACAAGAGAAGAAGGAAAATGATTTAGGAGAGATGAAGAAAGAGGAAGATGAGAAGCAATTGAAATATGAACCTGATGAGGTAGAAAGCCAATGCAAACAGGAAAATTCACAGCCTCAAAGTAACAACAACAATGCAACTTCTTCAAATGATAGAATTACTGATCCAAAGAGAGTCAAAAGGTAATTATCAAACACTTCTATATACTATAGATAAAAATTAAAACATGTAGGATGAAATTTAAAACAATTTTTTTTTTCTTCTAATTTTCTTGCATAATTCAACTTTTTCTAACAGTATATTTTTATCTATATACATATGCTTAATTTTGCTAATAAACTTGCAAAGCCCACATGACACAATTTTTTAGTACTTCATTATTGTAGATTATTTTTTTAAGCATGATTTTGATATTCTAGTTGAAATTGTTGATTAGTTTTCATGCATTGCTCTTTATAATTGAAATATTGTTGATCTTGGTTTCTTGAACTTTTTATATATTTTTTTCTTTGTTTGTAAATGAAAATAAAGCATTTTTTTCCTTTGAGTTTTGCAACTGATTCTGGTTTTTTTGCATTAGAATCTTGGCAAATAGGCAATCAGCACAAAGGTCACGTGTGAGGAAGCTGCAATACATATCAGAGCTTGAGCGTAGTGTAACTTCATTGCAGGTTTGTTACGTAACATAACATGATTTCACTATAATTTTTGTATTATTTATTTGCAGTACTATAATATCTGGAAATTTAATGTATTATGTATATAGTAGAAATGATCAAATAATAATGTTATTGACTTTTTGTTATTCCTTCTACTGTCCAAATATATATTGAAGGCTGAAGTTTCAGTGCTATCTCCAAGAGTTGCATTTTTGGATCATCAACGATTGCTTCTAAATGTTGACAACAGTGCTCTCAAGCAAAGAATTGCAGCCCTTGCACAAGACAAGCTTTTCAAAGATGGTAATTTAATTATTTCTTCAATACTATATTGTTCAATTTAATTATTATTACTAATTAGTGAATTTATATGGATATATGAGTGAAAGAATATGTACCCTGTGGTCCTAATGAGACCATGACAAGTCAAGCATGAAATGACAAAATAAAAATGGAGAAAACACACCAATAAGCTATATATAGCACTGTAGTTACTTGAAGTTGGTCAAGTATTCACTAGTAGTAAATTAAATTTAATAAGAGTGAAACAGGTCCATAGTTATGAGACTGCACACAGAGTTATAATGAATTTAGTGAATGTGTTTTTGTTTGCATGCACGCCTATTTATGTGATCATGTGCCCCCATCACAAAGTAGTTAATATACTCAAAAAGTCTACTAACATTTGAGCTCGGGTAATAGTCATTTTTGTCTTTGAATATGTAACAAATATAGTCCTATGTATCAAAATTTCAAGAGTTTCTAATTGTACAATATGTTAGTCAAAATAGTTTCTTAGGTTAAAATGTCTCGTTAATATTGTAATAATAGTTCTTCAATATACTTATTTATTGTCATATCAATCTCTATATAAAAAGAATTAATATCAATATTGAAGGATTATTGTGCTTACTCGTTAGGGACTAAAATGACTATACTCCATTTGAACGCACACAAACATAAAAATACTATCTTTTAATAATTTTAAAAATATATATTATTTTATTTTATTTCTTTTTTCATCTTTTATTAAGTGTGTGAATGTGTCCAAATAGAATGTACAAATACTTGTGACCAACTAAGTATTTTTCAAATATACTTGTCTAGTGTCCTGCCTCAAAGTAGTTAGCTCCTTTTTACTTATCAGATTTAAAGTTGTTTTGTACTGTTAGTAGTTGTTATTTTTTTAAGTTTAATGTAACATTAGTTATTGTTTTATTAATAATAATTACTGGCCAATCATTGTTTTTCATTAATTATTGAATATCCTTAATAGTTAATACTATTTAGACACATAGCTAGGGTGAAGCTTCAGATATTGTACTTACTATAATGACATTTATATATTTACATGCAGCTCATCAAGAGGCATTGAAGAGGGAGATAGAAAGATTAAGACAGGTATATCACCATCAGAACCTCAAGAAGATGGAAAATGCTAATGGATCACCCTTACCATCCCCATCACAAAAGCCAATATGTGATTCTCAAACAGAAAGCGAACAACTACTCAATGTATGA
Protein sequence

>MS.gene31409.t1

MAQLPPKIPNMSPSWPDFSKHQKNIMPTSISTNNIVTTNNYQNQNPSWVDEFLDFSSARRGAHRRSVSDSVTFLETPMLDDCRGKHDGNNNDNEFDKFDDEQFLSMFSDEISGVNNMPPTLSSSNPSSPSDQNFVNDDNEKEKNKQEKKENDLGEMKKEEDEKQLKYEPDEVESQCKQENSQPQSNNNNATSSNDRITDPKRVKRILANRQSAQRSRVRKLQYISELERSVTSLQAEVSVLSPRVAFLDHQRLLLNVDNSALKQRIAALAQDKLFKDAHQEALKREIERLRQVYHHQNLKKMENANGSPLPSPSQKPICDSQTESEQLLNV