Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04398.t1 | XP_003600475.1 | 99.2 | 244 | 2 | 0 | 1 | 244 | 16 | 259 | 2.90E-135 | 491.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04398.t1 | Q8C5Q4 | 33.2 | 226 | 108 | 4 | 43 | 229 | 250 | 471 | 9.0e-24 | 112.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04398.t1 | G7J263 | 99.2 | 244 | 2 | 0 | 1 | 244 | 16 | 259 | 2.1e-135 | 491.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene33973 | MS.gene04398 | PPI |
| MS.gene006028 | MS.gene04398 | PPI |
| MS.gene60814 | MS.gene04398 | PPI |
| MS.gene04398 | MS.gene041727 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04398.t1 | MTR_3g061590 | 99.180 | 244 | 2 | 0 | 1 | 244 | 16 | 259 | 3.25e-180 | 494 |
| MS.gene04398.t1 | MTR_8g073580 | 50.199 | 251 | 113 | 4 | 1 | 244 | 44 | 289 | 4.70e-72 | 222 |
| MS.gene04398.t1 | MTR_8g073580 | 52.632 | 209 | 88 | 3 | 43 | 244 | 38 | 242 | 2.91e-71 | 218 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04398.t1 | AT3G20890 | 61.786 | 280 | 69 | 4 | 1 | 244 | 15 | 292 | 3.41e-107 | 311 |
| MS.gene04398.t1 | AT3G20890 | 59.113 | 203 | 50 | 1 | 75 | 244 | 27 | 229 | 6.47e-80 | 239 |
| MS.gene04398.t1 | AT5G66010 | 48.729 | 236 | 108 | 5 | 1 | 232 | 16 | 242 | 3.91e-71 | 218 |
| MS.gene04398.t1 | AT5G66010 | 47.260 | 146 | 72 | 2 | 91 | 232 | 1 | 145 | 9.71e-43 | 142 |
Find 71 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGCGAAGGCTTCTGTGTTT+TGG | 0.180490 | 3.4:-57375045 | MS.gene04398:CDS |
| GACGCGGGATCTTCATTCTA+TGG | 0.326213 | 3.4:-57375257 | MS.gene04398:CDS |
| TCATCCTTATTAGCAGAAAA+TGG | 0.345506 | 3.4:+57374795 | None:intergenic |
| AATTCAGAGGGTAGACCATC+TGG | 0.348543 | 3.4:-57374714 | MS.gene04398:CDS |
| AGATGGATTGTACATATAAC+TGG | 0.382208 | 3.4:+57375223 | None:intergenic |
| ACTTTGCTCTTCAAAGAAAC+AGG | 0.406141 | 3.4:-57375004 | MS.gene04398:CDS |
| TGCTAATAAGGATGACATAA+TGG | 0.410596 | 3.4:-57374787 | MS.gene04398:CDS |
| TCTTCATTCTATGGTTCCCC+TGG | 0.414721 | 3.4:-57375248 | MS.gene04398:CDS |
| TCCATCTCACCATGCGTTGA+AGG | 0.422533 | 3.4:+57374597 | None:intergenic |
| CTACAAGGCAATTGCAAATG+AGG | 0.431623 | 3.4:-57374928 | MS.gene04398:CDS |
| CGCAGTCGAACAACAGGAAA+TGG | 0.434626 | 3.4:+57375167 | None:intergenic |
| AGACCACGCAGTCGAACAAC+AGG | 0.449245 | 3.4:+57375161 | None:intergenic |
| CTCCATCATATGATTTAGCT+CGG | 0.453352 | 3.4:+57374865 | None:intergenic |
| ACCATCTGGGGAGGCTTATG+TGG | 0.470749 | 3.4:-57374700 | MS.gene04398:CDS |
| CTTCAAGGAGTATGTGTTGT+CGG | 0.500765 | 3.4:-57374760 | MS.gene04398:CDS |
| CACAGGAGTGTTGCGACTGA+GGG | 0.505697 | 3.4:-57374823 | MS.gene04398:CDS |
| TCCATCATATGATTTAGCTC+GGG | 0.508153 | 3.4:+57374866 | None:intergenic |
| ATGTACAATCCATCTCCATA+TGG | 0.508179 | 3.4:-57375215 | MS.gene04398:CDS |
| CTCGGGGAGCACTACGACGA+GGG | 0.508597 | 3.4:+57374883 | None:intergenic |
| ACAGGAAATGGTGGAGGAGG+AGG | 0.509420 | 3.4:+57375179 | None:intergenic |
| ATATGATGGAGGAAAAGATT+CGG | 0.513132 | 3.4:-57374853 | MS.gene04398:CDS |
| ATCAGTTTCAGTGCAATCAA+AGG | 0.523371 | 3.4:+57375136 | None:intergenic |
| CCATCTCACCATGCGTTGAA+GGG | 0.532489 | 3.4:+57374598 | None:intergenic |
| TGACATAATGGAGTTCTTCA+AGG | 0.535855 | 3.4:-57374775 | MS.gene04398:CDS |
| CCCCGAGCTAAATCATATGA+TGG | 0.536873 | 3.4:-57374867 | MS.gene04398:CDS |
| GATGTGGCCGAGTTCTTCCA+TGG | 0.538399 | 3.4:-57375116 | MS.gene04398:CDS |
| CCCTTCAACGCATGGTGAGA+TGG | 0.538750 | 3.4:-57374598 | MS.gene04398:CDS |
| GCAAAGGATAGAATGACGCT+TGG | 0.544787 | 3.4:-57374642 | MS.gene04398:CDS |
| CGTCATTCTATCCTTTGCCA+TGG | 0.545218 | 3.4:+57374647 | None:intergenic |
| ATTCAGAGGGTAGACCATCT+GGG | 0.546301 | 3.4:-57374713 | MS.gene04398:CDS |
| TCAGTTTCAGTGCAATCAAA+GGG | 0.547953 | 3.4:+57375137 | None:intergenic |
| CAAAGGATAGAATGACGCTT+GGG | 0.551160 | 3.4:-57374641 | MS.gene04398:CDS |
| GGTTGGCTGACATATCCATA+TGG | 0.556296 | 3.4:+57375200 | None:intergenic |
| CAAAGAAACAGGCAGAACAT+AGG | 0.560049 | 3.4:-57374993 | MS.gene04398:CDS |
| CTGACATATCCATATGGAGA+TGG | 0.564910 | 3.4:+57375206 | None:intergenic |
| TGTACATATAACTGGAACCA+GGG | 0.571227 | 3.4:+57375231 | None:intergenic |
| TTTCCTGTTGTTCGACTGCG+TGG | 0.572149 | 3.4:-57375164 | MS.gene04398:CDS |
| AGAGCAAAGTCAACTTGAAG+AGG | 0.573046 | 3.4:+57375017 | None:intergenic |
| ACAACAGGAAATGGTGGAGG+AGG | 0.573282 | 3.4:+57375176 | None:intergenic |
| TCCACATAAGCCTCCCCAGA+TGG | 0.579302 | 3.4:+57374699 | None:intergenic |
| GTCTAGACCATGGAAGAACT+CGG | 0.581671 | 3.4:+57375109 | None:intergenic |
| GAGCTATTCCCTTCAACGCA+TGG | 0.586026 | 3.4:-57374606 | MS.gene04398:CDS |
| TGATGATTCAAAAGCAGCCA+TGG | 0.593984 | 3.4:-57374664 | MS.gene04398:CDS |
| ACACAGGAGTGTTGCGACTG+AGG | 0.596079 | 3.4:-57374824 | MS.gene04398:CDS |
| TTCAAAAGCAGCCATGGCAA+AGG | 0.597698 | 3.4:-57374658 | MS.gene04398:CDS |
| CCACATTGTAATGAATTCAG+AGG | 0.601918 | 3.4:-57374727 | MS.gene04398:CDS |
| CGAACAACAGGAAATGGTGG+AGG | 0.604157 | 3.4:+57375173 | None:intergenic |
| GAAATGGTGGAGGAGGAGGT+TGG | 0.608351 | 3.4:+57375183 | None:intergenic |
| ATGGAAGATGCAATTTCAAG+AGG | 0.609038 | 3.4:-57374579 | MS.gene04398:CDS |
| TGATTGCACTGAAACTGATG+TGG | 0.609327 | 3.4:-57375132 | MS.gene04398:CDS |
| CCATCATATGATTTAGCTCG+GGG | 0.614326 | 3.4:+57374867 | None:intergenic |
| CACATTGTAATGAATTCAGA+GGG | 0.621595 | 3.4:-57374726 | MS.gene04398:CDS |
| AAAGATTCGGCTGAGCACAC+AGG | 0.623512 | 3.4:-57374840 | MS.gene04398:CDS |
| ACGTCAGCGGACAGTTGACG+CGG | 0.625189 | 3.4:-57375273 | MS.gene04398:CDS |
| TCGGGGAGCACTACGACGAG+GGG | 0.629479 | 3.4:+57374884 | None:intergenic |
| TAAGAGGCAGGAATACTACA+AGG | 0.640162 | 3.4:-57374943 | MS.gene04398:CDS |
| TTCAGAGGGTAGACCATCTG+GGG | 0.650728 | 3.4:-57374712 | MS.gene04398:CDS |
| CAAGGAGTATGTGTTGTCGG+AGG | 0.654683 | 3.4:-57374757 | MS.gene04398:CDS |
| CGTCAGCGGACAGTTGACGC+GGG | 0.660169 | 3.4:-57375272 | MS.gene04398:CDS |
| CGAGCTAAATCATATGATGG+AGG | 0.660288 | 3.4:-57374864 | MS.gene04398:CDS |
| TTGTACATATAACTGGAACC+AGG | 0.660817 | 3.4:+57375230 | None:intergenic |
| CATCAACTATGTCTAGACCA+TGG | 0.660852 | 3.4:+57375099 | None:intergenic |
| GCTCGGGGAGCACTACGACG+AGG | 0.665801 | 3.4:+57374882 | None:intergenic |
| CCTCTGAATTCATTACAATG+TGG | 0.671128 | 3.4:+57374727 | None:intergenic |
| ACAGGAGTGTTGCGACTGAG+GGG | 0.684610 | 3.4:-57374822 | MS.gene04398:CDS |
| AGAGGGTAGACCATCTGGGG+AGG | 0.687416 | 3.4:-57374709 | MS.gene04398:CDS |
| AGTCGAACAACAGGAAATGG+TGG | 0.687976 | 3.4:+57375170 | None:intergenic |
| AATGAGGTTTCAGATGCTCG+AGG | 0.692222 | 3.4:-57374912 | MS.gene04398:CDS |
| AAATGGCTTCAAAACGTCAG+CGG | 0.695070 | 3.4:-57375286 | MS.gene04398:CDS |
| GAGGTTTCAGATGCTCGAGG+CGG | 0.695093 | 3.4:-57374909 | MS.gene04398:CDS |
| GTACATATAACTGGAACCAG+GGG | 0.705619 | 3.4:+57375232 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTACCATTTTCTGCTAATA+AGG | - | chr3.4:57375057-57375076 | MS.gene04398:CDS | 25.0% |
| CACATTGTAATGAATTCAGA+GGG | - | chr3.4:57375130-57375149 | MS.gene04398:CDS | 30.0% | |
| TCATCCTTATTAGCAGAAAA+TGG | + | chr3.4:57375064-57375083 | None:intergenic | 30.0% | |
| TGCTAATAAGGATGACATAA+TGG | - | chr3.4:57375069-57375088 | MS.gene04398:CDS | 30.0% | |
| ! | ATATGATGGAGGAAAAGATT+CGG | - | chr3.4:57375003-57375022 | MS.gene04398:CDS | 30.0% |
| ! | GATGTTCTTTTTGTTCACAA+AGG | - | chr3.4:57374776-57374795 | MS.gene04398:CDS | 30.0% |
| ! | TTGAAGTTTTCAGAAGTAAG+AGG | - | chr3.4:57374897-57374916 | MS.gene04398:CDS | 30.0% |
| !! | AGATGGATTGTACATATAAC+TGG | + | chr3.4:57374636-57374655 | None:intergenic | 30.0% |
| ACTTTGCTCTTCAAAGAAAC+AGG | - | chr3.4:57374852-57374871 | MS.gene04398:CDS | 35.0% | |
| ATCAGTTTCAGTGCAATCAA+AGG | + | chr3.4:57374723-57374742 | None:intergenic | 35.0% | |
| ATGGAAGATGCAATTTCAAG+AGG | - | chr3.4:57375277-57375296 | MS.gene04398:CDS | 35.0% | |
| ATGTACAATCCATCTCCATA+TGG | - | chr3.4:57374641-57374660 | MS.gene04398:CDS | 35.0% | |
| CCACATTGTAATGAATTCAG+AGG | - | chr3.4:57375129-57375148 | MS.gene04398:CDS | 35.0% | |
| CCTCTGAATTCATTACAATG+TGG | + | chr3.4:57375132-57375151 | None:intergenic | 35.0% | |
| CTCCATCATATGATTTAGCT+CGG | + | chr3.4:57374994-57375013 | None:intergenic | 35.0% | |
| TCAGTTTCAGTGCAATCAAA+GGG | + | chr3.4:57374722-57374741 | None:intergenic | 35.0% | |
| TGTACATATAACTGGAACCA+GGG | + | chr3.4:57374628-57374647 | None:intergenic | 35.0% | |
| TTGTACATATAACTGGAACC+AGG | + | chr3.4:57374629-57374648 | None:intergenic | 35.0% | |
| ! | GTTCTTTTTGTTCACAAAGG+TGG | - | chr3.4:57374779-57374798 | MS.gene04398:CDS | 35.0% |
| ! | TCCATCATATGATTTAGCTC+GGG | + | chr3.4:57374993-57375012 | None:intergenic | 35.0% |
| ! | TGACATAATGGAGTTCTTCA+AGG | - | chr3.4:57375081-57375100 | MS.gene04398:CDS | 35.0% |
| AGAGCAAAGTCAACTTGAAG+AGG | + | chr3.4:57374842-57374861 | None:intergenic | 40.0% | |
| CAAAGAAACAGGCAGAACAT+AGG | - | chr3.4:57374863-57374882 | MS.gene04398:CDS | 40.0% | |
| CAAAGGATAGAATGACGCTT+GGG | - | chr3.4:57375215-57375234 | MS.gene04398:CDS | 40.0% | |
| CATCAACTATGTCTAGACCA+TGG | + | chr3.4:57374760-57374779 | None:intergenic | 40.0% | |
| CGAGCTAAATCATATGATGG+AGG | - | chr3.4:57374992-57375011 | MS.gene04398:CDS | 40.0% | |
| CTACAAGGCAATTGCAAATG+AGG | - | chr3.4:57374928-57374947 | MS.gene04398:CDS | 40.0% | |
| CTGACATATCCATATGGAGA+TGG | + | chr3.4:57374653-57374672 | None:intergenic | 40.0% | |
| GTACATATAACTGGAACCAG+GGG | + | chr3.4:57374627-57374646 | None:intergenic | 40.0% | |
| TAAGAGGCAGGAATACTACA+AGG | - | chr3.4:57374913-57374932 | MS.gene04398:CDS | 40.0% | |
| TGATGATTCAAAAGCAGCCA+TGG | - | chr3.4:57375192-57375211 | MS.gene04398:CDS | 40.0% | |
| TGATTGCACTGAAACTGATG+TGG | - | chr3.4:57374724-57374743 | MS.gene04398:CDS | 40.0% | |
| ! | AGTTTTCAGAAGTAAGAGGC+AGG | - | chr3.4:57374901-57374920 | MS.gene04398:CDS | 40.0% |
| ! | CCATCATATGATTTAGCTCG+GGG | + | chr3.4:57374992-57375011 | None:intergenic | 40.0% |
| ! | CTTCAAGGAGTATGTGTTGT+CGG | - | chr3.4:57375096-57375115 | MS.gene04398:CDS | 40.0% |
| AATGAGGTTTCAGATGCTCG+AGG | - | chr3.4:57374944-57374963 | MS.gene04398:CDS | 45.0% | |
| AATTCAGAGGGTAGACCATC+TGG | - | chr3.4:57375142-57375161 | MS.gene04398:CDS | 45.0% | |
| AGTCGAACAACAGGAAATGG+TGG | + | chr3.4:57374689-57374708 | None:intergenic | 45.0% | |
| ATTCAGAGGGTAGACCATCT+GGG | - | chr3.4:57375143-57375162 | MS.gene04398:CDS | 45.0% | |
| CCCCGAGCTAAATCATATGA+TGG | - | chr3.4:57374989-57375008 | MS.gene04398:CDS | 45.0% | |
| CGTCATTCTATCCTTTGCCA+TGG | + | chr3.4:57375212-57375231 | None:intergenic | 45.0% | |
| GCAAAGGATAGAATGACGCT+TGG | - | chr3.4:57375214-57375233 | MS.gene04398:CDS | 45.0% | |
| GTCTAGACCATGGAAGAACT+CGG | + | chr3.4:57374750-57374769 | None:intergenic | 45.0% | |
| TCTTCATTCTATGGTTCCCC+TGG | - | chr3.4:57374608-57374627 | MS.gene04398:CDS | 45.0% | |
| TTCAAAAGCAGCCATGGCAA+AGG | - | chr3.4:57375198-57375217 | MS.gene04398:CDS | 45.0% | |
| ! | CACAAAGGTGGCAAGTTTTC+TGG | - | chr3.4:57374791-57374810 | MS.gene04398:CDS | 45.0% |
| ! | GGTTGGCTGACATATCCATA+TGG | + | chr3.4:57374659-57374678 | None:intergenic | 45.0% |
| AAAGATTCGGCTGAGCACAC+AGG | - | chr3.4:57375016-57375035 | MS.gene04398:CDS | 50.0% | |
| CCATCTCACCATGCGTTGAA+GGG | + | chr3.4:57375261-57375280 | None:intergenic | 50.0% | |
| CGAACAACAGGAAATGGTGG+AGG | + | chr3.4:57374686-57374705 | None:intergenic | 50.0% | |
| CGCAGTCGAACAACAGGAAA+TGG | + | chr3.4:57374692-57374711 | None:intergenic | 50.0% | |
| GACGCGGGATCTTCATTCTA+TGG | - | chr3.4:57374599-57374618 | MS.gene04398:CDS | 50.0% | |
| GAGCTATTCCCTTCAACGCA+TGG | - | chr3.4:57375250-57375269 | MS.gene04398:CDS | 50.0% | |
| TCCATCTCACCATGCGTTGA+AGG | + | chr3.4:57375262-57375281 | None:intergenic | 50.0% | |
| TTCAGAGGGTAGACCATCTG+GGG | - | chr3.4:57375144-57375163 | MS.gene04398:CDS | 50.0% | |
| TTTCCTGTTGTTCGACTGCG+TGG | - | chr3.4:57374692-57374711 | MS.gene04398:CDS | 50.0% | |
| ! | ACAACAGGAAATGGTGGAGG+AGG | + | chr3.4:57374683-57374702 | None:intergenic | 50.0% |
| ! | CAAGGAGTATGTGTTGTCGG+AGG | - | chr3.4:57375099-57375118 | MS.gene04398:CDS | 50.0% |
| ! | GGCGAAGGCTTCTGTGTTTT+GGG | - | chr3.4:57374812-57374831 | MS.gene04398:CDS | 50.0% |
| ! | TGGCGAAGGCTTCTGTGTTT+TGG | - | chr3.4:57374811-57374830 | MS.gene04398:CDS | 50.0% |
| ACCATCTGGGGAGGCTTATG+TGG | - | chr3.4:57375156-57375175 | MS.gene04398:CDS | 55.0% | |
| AGACCACGCAGTCGAACAAC+AGG | + | chr3.4:57374698-57374717 | None:intergenic | 55.0% | |
| CCCTTCAACGCATGGTGAGA+TGG | - | chr3.4:57375258-57375277 | MS.gene04398:CDS | 55.0% | |
| GAGGTTTCAGATGCTCGAGG+CGG | - | chr3.4:57374947-57374966 | MS.gene04398:CDS | 55.0% | |
| GATGTGGCCGAGTTCTTCCA+TGG | - | chr3.4:57374740-57374759 | MS.gene04398:CDS | 55.0% | |
| TCCACATAAGCCTCCCCAGA+TGG | + | chr3.4:57375160-57375179 | None:intergenic | 55.0% | |
| ! | ACAGGAAATGGTGGAGGAGG+AGG | + | chr3.4:57374680-57374699 | None:intergenic | 55.0% |
| ! | GAAATGGTGGAGGAGGAGGT+TGG | + | chr3.4:57374676-57374695 | None:intergenic | 55.0% |
| !! | ACACAGGAGTGTTGCGACTG+AGG | - | chr3.4:57375032-57375051 | MS.gene04398:CDS | 55.0% |
| !! | ACAGGAGTGTTGCGACTGAG+GGG | - | chr3.4:57375034-57375053 | MS.gene04398:CDS | 55.0% |
| !! | CACAGGAGTGTTGCGACTGA+GGG | - | chr3.4:57375033-57375052 | MS.gene04398:CDS | 55.0% |
| !! | GGTGGCAAGTTTTCTGGCGA+AGG | - | chr3.4:57374797-57374816 | MS.gene04398:CDS | 55.0% |
| ACGTCAGCGGACAGTTGACG+CGG | - | chr3.4:57374583-57374602 | MS.gene04398:CDS | 60.0% | |
| AGAGGGTAGACCATCTGGGG+AGG | - | chr3.4:57375147-57375166 | MS.gene04398:CDS | 60.0% | |
| CGTCAGCGGACAGTTGACGC+GGG | - | chr3.4:57374584-57374603 | MS.gene04398:CDS | 65.0% | |
| !! | CTCGGGGAGCACTACGACGA+GGG | + | chr3.4:57374976-57374995 | None:intergenic | 65.0% |
| !! | TCGGGGAGCACTACGACGAG+GGG | + | chr3.4:57374975-57374994 | None:intergenic | 65.0% |
| !! | GCTCGGGGAGCACTACGACG+AGG | + | chr3.4:57374977-57374996 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 57374572 | 57375306 | 57374572 | ID=MS.gene04398 |
| chr3.4 | mRNA | 57374572 | 57375306 | 57374572 | ID=MS.gene04398.t1;Parent=MS.gene04398 |
| chr3.4 | exon | 57374572 | 57375306 | 57374572 | ID=MS.gene04398.t1.exon1;Parent=MS.gene04398.t1 |
| chr3.4 | CDS | 57374572 | 57375306 | 57374572 | ID=cds.MS.gene04398.t1;Parent=MS.gene04398.t1 |
| Gene Sequence |
| Protein sequence |
