AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene044280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044280.t1 MTR_3g080690 96.419 391 14 0 1 391 55 445 0.0 794
MS.gene044280.t1 MTR_3g081240 95.420 393 16 1 1 391 55 447 0.0 786
MS.gene044280.t1 MTR_3g097310 63.802 384 137 1 7 390 110 491 9.33e-179 506
MS.gene044280.t1 MTR_1g061760 58.961 385 151 2 7 390 106 484 4.01e-167 477
MS.gene044280.t1 MTR_7g103260 56.136 383 158 2 7 387 20 394 7.52e-162 460
MS.gene044280.t1 MTR_7g103270 56.104 385 159 2 6 388 20 396 1.21e-159 454
MS.gene044280.t1 MTR_3g106690 59.172 338 135 2 54 388 52 389 1.03e-146 421
MS.gene044280.t1 MTR_4g115540 51.804 388 179 3 6 387 236 621 5.36e-140 412
MS.gene044280.t1 MTR_4g115460 50.382 393 185 6 2 388 72 460 2.32e-135 395
MS.gene044280.t1 MTR_4g115530 48.969 388 189 4 6 387 3 387 4.32e-130 379
MS.gene044280.t1 MTR_4g115530 50.150 333 160 2 61 387 1 333 2.26e-115 340
MS.gene044280.t1 MTR_4g100730 46.240 359 183 5 36 387 178 533 1.49e-109 332
MS.gene044280.t1 MTR_1g006600 45.596 386 193 4 5 386 211 583 1.62e-109 333
MS.gene044280.t1 MTR_7g103150 44.041 386 199 5 5 386 109 481 1.29e-107 325
MS.gene044280.t1 MTR_1g090733 43.915 378 193 7 16 387 181 545 8.58e-106 322
MS.gene044280.t1 MTR_8g102840 42.820 383 201 5 9 386 237 606 9.32e-106 324
MS.gene044280.t1 MTR_3g110198 46.221 344 178 3 47 386 250 590 6.40e-105 322
MS.gene044280.t1 MTR_3g110200 44.101 356 189 4 35 386 238 587 7.34e-102 313
MS.gene044280.t1 MTR_4g115540 50.498 301 141 3 6 300 236 534 5.44e-100 307
MS.gene044280.t1 MTR_5g031480 35.838 346 206 6 45 384 74 409 1.21e-71 230
MS.gene044280.t1 MTR_8g102840 39.597 298 162 5 9 301 237 521 3.91e-68 224
MS.gene044280.t1 MTR_5g049790 35.860 343 194 7 45 384 110 429 5.17e-67 219
MS.gene044280.t1 MTR_7g085840 35.882 340 191 10 61 388 1 325 4.64e-57 190
MS.gene044280.t1 MTR_5g031480 33.708 267 161 6 45 305 74 330 7.51e-42 150
MS.gene044280.t1 MTR_5g031480 33.835 266 160 6 45 304 74 329 2.08e-41 149
MS.gene044280.t1 MTR_5g049790 35.211 213 125 5 45 254 127 329 1.68e-31 122
MS.gene044280.t1 MTR_5g049790 35.211 213 125 5 45 254 110 312 2.26e-31 122
MS.gene044280.t1 MTR_8g096160 25.890 309 205 6 92 381 81 384 3.40e-28 114
MS.gene044280.t1 MTR_8g096140 27.010 311 199 8 92 381 81 384 2.37e-27 112
MS.gene044280.t1 MTR_2g084245 28.221 326 191 13 61 355 85 398 1.19e-23 102
MS.gene044280.t1 MTR_4g057500 26.836 354 210 14 63 381 105 444 3.33e-23 101
MS.gene044280.t1 MTR_3g037720 29.316 307 185 12 94 377 169 466 6.50e-22 97.8
MS.gene044280.t1 MTR_6g027310 26.578 301 198 10 95 381 174 465 1.08e-19 91.3
MS.gene044280.t1 MTR_8g080490 25.987 304 206 8 87 377 97 394 2.29e-19 89.7
MS.gene044280.t1 MTR_4g084200 24.825 286 185 10 102 367 158 433 3.24e-17 83.6
MS.gene044280.t1 MTR_3g085480 25.939 293 194 10 104 381 116 400 9.26e-17 82.0
MS.gene044280.t1 MTR_7g087100 25.510 294 192 10 104 381 188 470 1.79e-15 78.2
MS.gene044280.t1 MTR_4g084200 24.028 283 185 10 102 364 158 430 1.52e-14 75.1
MS.gene044280.t1 MTR_4g077920 25.503 298 192 10 94 367 97 388 3.03e-14 74.3
MS.gene044280.t1 MTR_4g084200 24.275 276 179 10 102 357 158 423 6.36e-14 73.2
MS.gene044280.t1 MTR_5g081290 30.464 151 87 3 61 211 1 133 8.86e-13 68.2
MS.gene044280.t1 MTR_1g097090 25.597 293 181 9 104 366 139 424 2.68e-12 68.6
MS.gene044280.t1 MTR_1g097090 25.597 293 181 9 104 366 139 424 2.79e-12 68.6
MS.gene044280.t1 MTR_1g061790 44.118 102 41 4 7 105 106 194 3.77e-12 65.5
MS.gene044280.t1 MTR_4g077920 25.877 228 148 8 156 367 224 446 1.05e-11 66.6
MS.gene044280.t1 MTR_4g077920 25.877 228 148 8 156 367 224 446 1.21e-11 66.6
MS.gene044280.t1 MTR_1g029350 27.612 268 165 12 96 346 118 373 1.47e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044280.t1 AT3G07620 68.325 382 118 3 9 388 76 456 0.0 551
MS.gene044280.t1 AT5G03795 57.772 386 157 1 6 391 137 516 3.69e-158 455
MS.gene044280.t1 AT5G25310 52.062 388 174 5 5 388 98 477 1.10e-144 419
MS.gene044280.t1 AT5G11130 50.254 394 176 5 9 390 91 476 2.61e-138 403
MS.gene044280.t1 AT5G11130 53.293 334 150 3 61 390 1 332 2.14e-129 375
MS.gene044280.t1 AT5G20260 49.476 382 187 5 10 388 85 463 5.66e-128 376
MS.gene044280.t1 AT3G42180 50.510 392 183 3 6 388 77 466 2.48e-127 375
MS.gene044280.t1 AT5G33290 50.278 360 168 3 38 388 139 496 9.36e-124 367
MS.gene044280.t1 AT3G42180 50.742 337 157 2 61 388 1 337 4.41e-118 347
MS.gene044280.t1 AT4G16745 46.354 384 189 6 11 387 156 529 9.00e-117 350
MS.gene044280.t1 AT5G20260 50.298 336 164 3 56 388 23 358 2.16e-115 340
MS.gene044280.t1 AT5G20260 50.453 331 161 3 61 388 1 331 4.76e-115 338
MS.gene044280.t1 AT4G32790 44.503 382 194 6 10 386 222 590 1.63e-106 326
MS.gene044280.t1 AT4G32790 44.503 382 194 6 10 386 222 590 1.63e-106 326
MS.gene044280.t1 AT4G32790 44.503 382 194 6 10 386 222 590 1.63e-106 326
MS.gene044280.t1 AT5G19670 44.348 345 184 3 47 386 263 604 5.59e-106 325
MS.gene044280.t1 AT5G19670 44.348 345 184 3 47 386 263 604 5.59e-106 325
MS.gene044280.t1 AT4G16745 44.744 371 188 6 11 374 156 516 1.26e-105 323
MS.gene044280.t1 AT5G25820 44.321 361 188 8 34 386 294 649 1.93e-98 306
MS.gene044280.t1 AT5G37000 43.188 345 187 4 47 386 208 548 7.65e-98 302
MS.gene044280.t1 AT5G11610 39.948 383 211 8 9 386 174 542 4.94e-96 297
MS.gene044280.t1 AT4G38040 35.549 346 207 6 45 384 84 419 3.43e-67 219
MS.gene044280.t1 AT5G11610 36.897 290 165 7 8 293 173 448 1.15e-59 200
MS.gene044280.t1 AT4G38040 34.161 322 196 6 45 360 84 395 1.19e-54 186
MS.gene044280.t1 AT5G11610 37.952 166 91 3 9 172 174 329 3.40e-31 122
MS.gene044280.t1 AT1G27440 26.214 309 204 7 92 381 77 380 2.13e-28 115
MS.gene044280.t1 AT5G61840 24.781 343 224 9 65 381 49 383 6.93e-26 108
MS.gene044280.t1 AT2G28110 26.367 311 200 10 104 391 138 442 9.79e-22 97.1
MS.gene044280.t1 AT1G74680 28.522 291 190 8 99 377 144 428 1.88e-21 96.3
MS.gene044280.t1 AT1G67410 27.211 294 196 9 98 377 113 402 3.28e-21 95.1
MS.gene044280.t1 AT5G22940 25.078 319 200 11 96 387 175 481 1.43e-19 90.9
MS.gene044280.t1 AT5G22940 25.078 319 200 11 96 387 146 452 1.66e-19 90.5
MS.gene044280.t1 AT3G45400 27.304 293 188 8 104 381 154 436 6.87e-19 88.6
MS.gene044280.t1 AT2G35100 24.912 285 181 10 102 367 125 395 1.97e-18 87.0
MS.gene044280.t1 AT3G03650 27.667 300 189 11 99 382 188 475 2.10e-17 84.3
MS.gene044280.t1 AT5G16890 24.211 285 186 11 100 367 181 452 3.80e-14 74.3
MS.gene044280.t1 AT5G44930 27.632 228 131 11 155 363 175 387 1.49e-12 69.3
MS.gene044280.t1 AT5G44930 27.632 228 131 11 155 363 175 387 1.49e-12 69.3
MS.gene044280.t1 AT1G34270 28.814 236 138 10 156 366 195 425 3.25e-11 65.1

Find 71 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAATATTTATCAACTTAT+TGG 0.144366 3.3:+68789917 MS.gene044280:CDS
CCTGTTACCAAGTTGATTTC+AGG 0.167077 3.3:-68790360 None:intergenic
TAAATTGCCTCAACAATTCT+TGG 0.244588 3.3:-68790609 None:intergenic
GGAAATGATGCATCTTTCTT+TGG 0.250368 3.3:-68790339 None:intergenic
ACAATTCTGGCTTTCTTTGC+AGG 0.251792 3.3:+68790426 MS.gene044280:CDS
TATTGTTGTGATATTATAAT+AGG 0.275605 3.3:-68790645 None:intergenic
ACAGCTTTGTCACGAATAAC+TGG 0.279821 3.3:-68790035 None:intergenic
ATATGATTATTCATTCTATA+TGG 0.288339 3.3:+68790859 MS.gene044280:CDS
TACTCATCTTCATTGTTTCA+TGG 0.291583 3.3:-68790548 None:intergenic
AGGATCATAACAACACTAAA+TGG 0.293875 3.3:-68789996 None:intergenic
CCTGAAATCAACTTGGTAAC+AGG 0.294494 3.3:+68790360 MS.gene044280:CDS
TCATGCTTTCTTGTCATGAT+TGG 0.298264 3.3:+68790141 MS.gene044280:CDS
ATATAGGAGTTACCACTTAA+TGG 0.323102 3.3:+68789809 MS.gene044280:intron
AATGCAAGGAGATAGAAATT+TGG 0.324834 3.3:+68788756 None:intergenic
CATGCTTTCTTGTCATGATT+GGG 0.337722 3.3:+68790142 MS.gene044280:CDS
AACAAAATTTCCTTAAGCTT+GGG 0.346625 3.3:-68790735 None:intergenic
TAGTGAAATTCCCAAGCTTA+AGG 0.365820 3.3:+68790725 MS.gene044280:CDS
ATTCGTGACAAAGCTGTATT+GGG 0.368021 3.3:+68790041 MS.gene044280:CDS
TGAAATACATCATATCTCTT+AGG 0.369121 3.3:-68790837 None:intergenic
CAATCAAGAACATCACTAAA+AGG 0.369286 3.3:-68790675 None:intergenic
CAACAAAATTTCCTTAAGCT+TGG 0.385217 3.3:-68790736 None:intergenic
TATTCGTGACAAAGCTGTAT+TGG 0.386148 3.3:+68790040 MS.gene044280:CDS
TATCACACAAATATGCATAT+TGG 0.394028 3.3:+68790093 MS.gene044280:CDS
AAGTAGACATGAGCTTCATT+TGG 0.398848 3.3:-68789969 None:intergenic
TTCACATACCATGTTGCCCT+TGG 0.411752 3.3:-68790255 None:intergenic
AGAAATTTGGAGAAGGTTGA+AGG 0.417058 3.3:+68788769 MS.gene044280:CDS
TAAATAGCTTCTCCATTAAG+TGG 0.424602 3.3:-68789821 None:intergenic
GCTGTATTGGGAAGAACTAT+TGG 0.445271 3.3:+68790053 MS.gene044280:CDS
TTATTCATTCTATATGGCTT+AGG 0.447096 3.3:+68790865 MS.gene044280:CDS
AACATGGAACAGAACAATTC+TGG 0.451636 3.3:+68790413 MS.gene044280:CDS
TATCTTTGTTCTTCCAATGT+TGG 0.453246 3.3:-68790482 None:intergenic
CATGACAAGAAAGCATGAAA+TGG 0.462671 3.3:-68790136 None:intergenic
CTTGCCACTTCCCACCCACT+TGG 0.464582 3.3:-68790585 None:intergenic
TTGCAAGAATATATATTCAA+TGG 0.476651 3.3:+68789893 MS.gene044280:CDS
GCATATTGGAATAGAAGTTA+TGG 0.477539 3.3:+68790107 MS.gene044280:CDS
AAGAGGCAGTACGGTGTCAT+TGG 0.482673 3.3:-68788839 None:intergenic
TTGGTAACAGGAGAAACAAC+AGG 0.491020 3.3:+68790372 MS.gene044280:CDS
TTCATTCTATATGGCTTAGG+AGG 0.491295 3.3:+68790868 MS.gene044280:CDS
GGAGAAACAACAGGTTTACT+TGG 0.502236 3.3:+68790381 MS.gene044280:CDS
TTGTCCAAGTGGGTGGGAAG+TGG 0.510845 3.3:+68790581 MS.gene044280:CDS
ATTGTATTTGTCCAAGTGGG+TGG 0.518103 3.3:+68790574 MS.gene044280:CDS
AGAATTGTTCTGTTCCATGT+TGG 0.536389 3.3:-68790411 None:intergenic
GAAATATATAAGATTGCAAG+AGG 0.540677 3.3:+68790773 MS.gene044280:CDS
ATCATTTCCTGAAATCAACT+TGG 0.557019 3.3:+68790353 MS.gene044280:CDS
CTGGGTGAAAGAGATGTTCA+AGG 0.557117 3.3:-68790016 None:intergenic
AGGAGATAGAAATTTGGAGA+AGG 0.557354 3.3:+68788762 MS.gene044280:CDS
AATACATCATATCTCTTAGG+AGG 0.557887 3.3:-68790834 None:intergenic
TTCCAATGTTGGAAAAGCAA+TGG 0.557955 3.3:-68790471 None:intergenic
TACTTGGTGGCTATCCAACA+TGG 0.562509 3.3:+68790397 MS.gene044280:CDS
GAAACAACAGGTTTACTTGG+TGG 0.562533 3.3:+68790384 MS.gene044280:CDS
ATTGAATATATATTCTTGCA+AGG 0.562749 3.3:-68789891 None:intergenic
CAAGAATATATATTCAATGG+AGG 0.575833 3.3:+68789896 MS.gene044280:CDS
GGTGTCATTGGTTCTCAACA+AGG 0.582461 3.3:-68788827 None:intergenic
ATCCTACCTATGAAAAGCAA+AGG 0.584468 3.3:-68788908 None:intergenic
AGAGTCCTCAAGAGGCAGTA+CGG 0.587025 3.3:-68788848 None:intergenic
TTGTATTTGTCCAAGTGGGT+GGG 0.588230 3.3:+68790575 MS.gene044280:CDS
AATATTGTATTTGTCCAAGT+GGG 0.593695 3.3:+68790571 MS.gene044280:CDS
ACTTTGCAATGCAAATATCT+CGG 0.598690 3.3:+68790305 MS.gene044280:CDS
TTCTTTGCAGGACAAATGCA+TGG 0.604295 3.3:+68790438 MS.gene044280:CDS
AAGAATATATATTCAATGGA+GGG 0.605925 3.3:+68789897 MS.gene044280:CDS
GGCAAGTCCAAGAATTGTTG+AGG 0.611209 3.3:+68790602 MS.gene044280:CDS
TAATCATGAGAGTCCTCAAG+AGG 0.613023 3.3:-68788856 None:intergenic
TTTCTGTAGATGTCTCCTTG+TGG 0.615421 3.3:-68788883 None:intergenic
AAATATTGTATTTGTCCAAG+TGG 0.615905 3.3:+68790570 MS.gene044280:CDS
TATAGGGACCAAGGGCAACA+TGG 0.622985 3.3:+68790247 MS.gene044280:intron
TGACACCGTACTGCCTCTTG+AGG 0.624877 3.3:+68788843 MS.gene044280:CDS
CAGCTTTGTCACGAATAACT+GGG 0.626719 3.3:-68790034 None:intergenic
AAGGGCAACATGGTATGTGA+AGG 0.631000 3.3:+68790257 MS.gene044280:CDS
CCTATAAGAAGATCAATGCA+AGG 0.636919 3.3:+68788742 None:intergenic
AAATATATAAGATTGCAAGA+GGG 0.689156 3.3:+68790774 MS.gene044280:CDS
TCTCATGATTATGTTCCACA+AGG 0.699811 3.3:+68788868 MS.gene044280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTATATTTCATGAAAA+TGG + chr3.3:68790217-68790236 MS.gene044280:intron 10.0%
!! ATATGATTATTCATTCTATA+TGG + chr3.3:68790859-68790878 MS.gene044280:CDS 15.0%
!! TATTGTTGTGATATTATAAT+AGG - chr3.3:68790648-68790667 None:intergenic 15.0%
!!! TTTTTACATAATGATGATAT+AGG + chr3.3:68789793-68789812 MS.gene044280:intron 15.0%
!! AAATATATAAGATTGCAAGA+GGG + chr3.3:68790774-68790793 MS.gene044280:CDS 20.0%
!! AAGAATATATATTCAATGGA+GGG + chr3.3:68789897-68789916 MS.gene044280:CDS 20.0%
!! AGTTACAACATTATTGAATA+TGG + chr3.3:68789384-68789403 MS.gene044280:intron 20.0%
!! ATTGAATATATATTCTTGCA+AGG - chr3.3:68789894-68789913 None:intergenic 20.0%
!! GATTCAAATTCATTTAACAT+AGG + chr3.3:68789444-68789463 MS.gene044280:intron 20.0%
!! TTAAAAATAGTTTCTTAGCT+TGG + chr3.3:68789537-68789556 MS.gene044280:intron 20.0%
!! TTGCAAGAATATATATTCAA+TGG + chr3.3:68789893-68789912 MS.gene044280:CDS 20.0%
!!! AAAATTTTTGTGTATGAAGA+GGG + chr3.3:68789843-68789862 MS.gene044280:CDS 20.0%
!!! TAAAATTTTTGTGTATGAAG+AGG + chr3.3:68789842-68789861 MS.gene044280:CDS 20.0%
!!! TTTTTTTGAACAATCAAAAG+TGG + chr3.3:68789133-68789152 MS.gene044280:intron 20.0%
! AAATATTGTATTTGTCCAAG+TGG + chr3.3:68790570-68790589 MS.gene044280:CDS 25.0%
! AACAAAATTTCCTTAAGCTT+GGG - chr3.3:68790738-68790757 None:intergenic 25.0%
! AACATTATTGAATATGGAGT+TGG + chr3.3:68789390-68789409 MS.gene044280:intron 25.0%
! AATATTGTATTTGTCCAAGT+GGG + chr3.3:68790571-68790590 MS.gene044280:CDS 25.0%
! ATCTCAACCACATATTAAAT+AGG - chr3.3:68789676-68789695 None:intergenic 25.0%
! ATTGAAGCCTATTTAATATG+TGG + chr3.3:68789666-68789685 MS.gene044280:intron 25.0%
! CAAGAATATATATTCAATGG+AGG + chr3.3:68789896-68789915 MS.gene044280:CDS 25.0%
! CATACTCACACTATAATTAA+CGG - chr3.3:68790189-68790208 None:intergenic 25.0%
! CCTTCAAAAAAAAATGTAAG+AGG - chr3.3:68789722-68789741 None:intergenic 25.0%
! GAAATATATAAGATTGCAAG+AGG + chr3.3:68790773-68790792 MS.gene044280:CDS 25.0%
! GAGGTTAAAACTCTTAAATA+TGG - chr3.3:68789703-68789722 None:intergenic 25.0%
! GGGAATATTTATCAACTTAT+TGG + chr3.3:68789917-68789936 MS.gene044280:CDS 25.0%
! TATCACACAAATATGCATAT+TGG + chr3.3:68790093-68790112 MS.gene044280:CDS 25.0%
! TGAAATACATCATATCTCTT+AGG - chr3.3:68790840-68790859 None:intergenic 25.0%
! TGAGAACACAAATGTTAAAT+GGG - chr3.3:68789569-68789588 None:intergenic 25.0%
! TTATTCATTCTATATGGCTT+AGG + chr3.3:68790865-68790884 MS.gene044280:CDS 25.0%
!! AAGCTTAAGGAAATTTTGTT+GGG + chr3.3:68790738-68790757 MS.gene044280:CDS 25.0%
!! TCATGGTATGAAATATTTTC+TGG - chr3.3:68790534-68790553 None:intergenic 25.0%
!! TTTTAGTGATGTTCTTGATT+GGG + chr3.3:68790677-68790696 MS.gene044280:CDS 25.0%
!!! ATGAAAATGGTTTTGTGTAT+AGG + chr3.3:68790230-68790249 MS.gene044280:intron 25.0%
!!! CCTCTTACATTTTTTTTTGA+AGG + chr3.3:68789719-68789738 MS.gene044280:intron 25.0%
!!! TGAAAATGGTTTTGTGTATA+GGG + chr3.3:68790231-68790250 MS.gene044280:intron 25.0%
AAAGATAGCTCAAAAGATGA+AGG - chr3.3:68789637-68789656 None:intergenic 30.0%
AAATCCACCTAATTGATATC+AGG + chr3.3:68789053-68789072 MS.gene044280:intron 30.0%
AATACATCATATCTCTTAGG+AGG - chr3.3:68790837-68790856 None:intergenic 30.0%
ACTTTGCAATGCAAATATCT+CGG + chr3.3:68790305-68790324 MS.gene044280:CDS 30.0%
AGGATCATAACAACACTAAA+TGG - chr3.3:68789999-68790018 None:intergenic 30.0%
ATATAGGAGTTACCACTTAA+TGG + chr3.3:68789809-68789828 MS.gene044280:intron 30.0%
ATCATTTCCTGAAATCAACT+TGG + chr3.3:68790353-68790372 MS.gene044280:CDS 30.0%
CAACAAAATTTCCTTAAGCT+TGG - chr3.3:68790739-68790758 None:intergenic 30.0%
CAATCAAGAACATCACTAAA+AGG - chr3.3:68790678-68790697 None:intergenic 30.0%
GAACTATATCATGAGAGATA+AGG + chr3.3:68788936-68788955 MS.gene044280:intron 30.0%
GCATATTGGAATAGAAGTTA+TGG + chr3.3:68790107-68790126 MS.gene044280:CDS 30.0%
GTATTAGAGTTTACGTTCAA+AGG + chr3.3:68789102-68789121 MS.gene044280:intron 30.0%
GTGAGAACACAAATGTTAAA+TGG - chr3.3:68789570-68789589 None:intergenic 30.0%
TAAATAGCTTCTCCATTAAG+TGG - chr3.3:68789824-68789843 None:intergenic 30.0%
TAAATTGCCTCAACAATTCT+TGG - chr3.3:68790612-68790631 None:intergenic 30.0%
TAATACAACTTCGATATTGC+AGG - chr3.3:68789088-68789107 None:intergenic 30.0%
TACTCATCTTCATTGTTTCA+TGG - chr3.3:68790551-68790570 None:intergenic 30.0%
TATCTTTGTTCTTCCAATGT+TGG - chr3.3:68790485-68790504 None:intergenic 30.0%
TGAAATATATGTCTTTAGCC+TGG - chr3.3:68789339-68789358 None:intergenic 30.0%
TTCACTTTATCTAACAACCT+GGG + chr3.3:68789355-68789374 MS.gene044280:intron 30.0%
TTTCACTTTATCTAACAACC+TGG + chr3.3:68789354-68789373 MS.gene044280:intron 30.0%
! ACTTTGCTATGTTACTTAAG+TGG - chr3.3:68789001-68789020 None:intergenic 30.0%
! CAAGCTTAAGGAAATTTTGT+TGG + chr3.3:68790737-68790756 MS.gene044280:CDS 30.0%
! CATCTTTTGAGCTATCTTTT+GGG + chr3.3:68789638-68789657 MS.gene044280:intron 30.0%
! CTTTTAGTGATGTTCTTGAT+TGG + chr3.3:68790676-68790695 MS.gene044280:CDS 30.0%
! TCATCTTTTGAGCTATCTTT+TGG + chr3.3:68789637-68789656 MS.gene044280:intron 30.0%
! TGTTCTTACGGTAATTCTTT+TGG - chr3.3:68789204-68789223 None:intergenic 30.0%
AACATGGAACAGAACAATTC+TGG + chr3.3:68790413-68790432 MS.gene044280:CDS 35.0%
AAGTAGACATGAGCTTCATT+TGG - chr3.3:68789972-68789991 None:intergenic 35.0%
AATAGAGCATGACATGTAAG+AGG - chr3.3:68789754-68789773 None:intergenic 35.0%
AGAAATTTGGAGAAGGTTGA+AGG + chr3.3:68788769-68788788 MS.gene044280:CDS 35.0%
AGAATTGTTCTGTTCCATGT+TGG - chr3.3:68790414-68790433 None:intergenic 35.0%
AGGAGATAGAAATTTGGAGA+AGG + chr3.3:68788762-68788781 MS.gene044280:CDS 35.0%
ATCCTACCTATGAAAAGCAA+AGG - chr3.3:68788911-68788930 None:intergenic 35.0%
ATGTTGTAACTTCTATTCCC+AGG - chr3.3:68789375-68789394 None:intergenic 35.0%
ATTCGTGACAAAGCTGTATT+GGG + chr3.3:68790041-68790060 MS.gene044280:CDS 35.0%
CATGCTTTCTTGTCATGATT+GGG + chr3.3:68790142-68790161 MS.gene044280:CDS 35.0%
CGATTCTCCTGATATCAATT+AGG - chr3.3:68789063-68789082 None:intergenic 35.0%
GGAAATGATGCATCTTTCTT+TGG - chr3.3:68790342-68790361 None:intergenic 35.0%
TAAAGTTAGCTCACAAGATG+AGG - chr3.3:68789420-68789439 None:intergenic 35.0%
TAGTGAAATTCCCAAGCTTA+AGG + chr3.3:68790725-68790744 MS.gene044280:CDS 35.0%
TATTCGTGACAAAGCTGTAT+TGG + chr3.3:68790040-68790059 MS.gene044280:CDS 35.0%
TCATGCTTTCTTGTCATGAT+TGG + chr3.3:68790141-68790160 MS.gene044280:CDS 35.0%
TCATGTATGACGTGAAACTT+GGG - chr3.3:68789508-68789527 None:intergenic 35.0%
TCTCATGATTATGTTCCACA+AGG + chr3.3:68788868-68788887 MS.gene044280:CDS 35.0%
TTCATGTATGACGTGAAACT+TGG - chr3.3:68789509-68789528 None:intergenic 35.0%
TTCATTCTATATGGCTTAGG+AGG + chr3.3:68790868-68790887 MS.gene044280:CDS 35.0%
TTCCAATGTTGGAAAAGCAA+TGG - chr3.3:68790474-68790493 None:intergenic 35.0%
TTCTCCTGATATCAATTAGG+TGG - chr3.3:68789060-68789079 None:intergenic 35.0%
! AATTTTGTTGGGTATCTCTG+AGG + chr3.3:68790749-68790768 MS.gene044280:CDS 35.0%
! CATGACAAGAAAGCATGAAA+TGG - chr3.3:68790139-68790158 None:intergenic 35.0%
! TGACTGACTCTTTGTTCTTA+CGG - chr3.3:68789216-68789235 None:intergenic 35.0%
!! GAAATGCCTTTGCTTTTCAT+AGG + chr3.3:68788902-68788921 MS.gene044280:CDS 35.0%
AGCTCAATCACTTGGTTATG+AGG - chr3.3:68789600-68789619 None:intergenic 40.0%
ATTGTATTTGTCCAAGTGGG+TGG + chr3.3:68790574-68790593 MS.gene044280:CDS 40.0%
CAAGGACCATAGTGAAAAAG+AGG - chr3.3:68789876-68789895 None:intergenic 40.0%
GAAACAACAGGTTTACTTGG+TGG + chr3.3:68790384-68790403 MS.gene044280:CDS 40.0%
GCTGTATTGGGAAGAACTAT+TGG + chr3.3:68790053-68790072 MS.gene044280:CDS 40.0%
GGAGAAACAACAGGTTTACT+TGG + chr3.3:68790381-68790400 MS.gene044280:CDS 40.0%
TAATCATGAGAGTCCTCAAG+AGG - chr3.3:68788859-68788878 None:intergenic 40.0%
TTCTTTGCAGGACAAATGCA+TGG + chr3.3:68790438-68790457 MS.gene044280:CDS 40.0%
TTGTATTTGTCCAAGTGGGT+GGG + chr3.3:68790575-68790594 MS.gene044280:CDS 40.0%
TTTCTGTAGATGTCTCCTTG+TGG - chr3.3:68788886-68788905 None:intergenic 40.0%
! AAAAAGGCTGCCTTTAGGTT+TGG - chr3.3:68789307-68789326 None:intergenic 40.0%
! AAACCTAAAGGCAGCCTTTT+TGG + chr3.3:68789306-68789325 MS.gene044280:intron 40.0%
! ACAGCTTTGTCACGAATAAC+TGG - chr3.3:68790038-68790057 None:intergenic 40.0%
! CAGCTTTGTCACGAATAACT+GGG - chr3.3:68790037-68790056 None:intergenic 40.0%
! CCTGAAATCAACTTGGTAAC+AGG + chr3.3:68790360-68790379 MS.gene044280:CDS 40.0%
! GTTTTGTGTATAGGGACCAA+GGG + chr3.3:68790239-68790258 MS.gene044280:intron 40.0%
! TTGGTAACAGGAGAAACAAC+AGG + chr3.3:68790372-68790391 MS.gene044280:CDS 40.0%
!! ACAATTCTGGCTTTCTTTGC+AGG + chr3.3:68790426-68790445 MS.gene044280:CDS 40.0%
!! CCTGTTACCAAGTTGATTTC+AGG - chr3.3:68790363-68790382 None:intergenic 40.0%
!! GAACCTCCTCTTTTTCACTA+TGG + chr3.3:68789867-68789886 MS.gene044280:CDS 40.0%
!! GACCATTGCTTTTCCAACAT+TGG + chr3.3:68790469-68790488 MS.gene044280:CDS 40.0%
!! TGCCTTTGCTTTTCATAGGT+AGG + chr3.3:68788906-68788925 MS.gene044280:intron 40.0%
!!! GCTAGATGAGTTGCTTTTTG+TGG - chr3.3:68789171-68789190 None:intergenic 40.0%
AAGCCAAAAAGGCTGCCTTT+AGG - chr3.3:68789312-68789331 None:intergenic 45.0%
AAGGGCAACATGGTATGTGA+AGG + chr3.3:68790257-68790276 MS.gene044280:CDS 45.0%
ACCAAGTGATTGAGCTCAAC+TGG + chr3.3:68789604-68789623 MS.gene044280:intron 45.0%
ACCAGTTGAGCTCAATCACT+TGG - chr3.3:68789608-68789627 None:intergenic 45.0%
AGCCTGGTTGAAAGCCAAAA+AGG - chr3.3:68789323-68789342 None:intergenic 45.0%
CCTATCTGTGCCAAACCTAA+AGG + chr3.3:68789294-68789313 MS.gene044280:intron 45.0%
CTGGGTGAAAGAGATGTTCA+AGG - chr3.3:68790019-68790038 None:intergenic 45.0%
GGACCATAGTGAAAAAGAGG+AGG - chr3.3:68789873-68789892 None:intergenic 45.0%
GGCAAGTCCAAGAATTGTTG+AGG + chr3.3:68790602-68790621 MS.gene044280:CDS 45.0%
TCAAGCCCGTTTCAATGCTT+GGG - chr3.3:68789268-68789287 None:intergenic 45.0%
TTAGGTTTGGCACAGATAGG+TGG - chr3.3:68789294-68789313 None:intergenic 45.0%
TTCACATACCATGTTGCCCT+TGG - chr3.3:68790258-68790277 None:intergenic 45.0%
! AATGCTTGGGCATCGCTTAA+TGG - chr3.3:68789255-68789274 None:intergenic 45.0%
! CCTTTAGGTTTGGCACAGAT+AGG - chr3.3:68789297-68789316 None:intergenic 45.0%
! TACTTGGTGGCTATCCAACA+TGG + chr3.3:68790397-68790416 MS.gene044280:CDS 45.0%
!! GGTGTCATTGGTTCTCAACA+AGG - chr3.3:68788830-68788849 None:intergenic 45.0%
!! GGTTTTGTGTATAGGGACCA+AGG + chr3.3:68790238-68790257 MS.gene044280:intron 45.0%
!!! AGCCTTTTTGGCTTTCAACC+AGG + chr3.3:68789318-68789337 MS.gene044280:intron 45.0%
AGAGTCCTCAAGAGGCAGTA+CGG - chr3.3:68788851-68788870 None:intergenic 50.0%
CTCAAGCCCGTTTCAATGCT+TGG - chr3.3:68789269-68789288 None:intergenic 50.0%
GCGCTAACTCAATCGTTAGG+TGG - chr3.3:68788979-68788998 None:intergenic 50.0%
GTGGCGCTAACTCAATCGTT+AGG - chr3.3:68788982-68789001 None:intergenic 50.0%
TATAGGGACCAAGGGCAACA+TGG + chr3.3:68790247-68790266 MS.gene044280:intron 50.0%
!! AAGAGGCAGTACGGTGTCAT+TGG - chr3.3:68788842-68788861 None:intergenic 50.0%
!! CGATGCCCAAGCATTGAAAC+GGG + chr3.3:68789260-68789279 MS.gene044280:intron 50.0%
!! GCGATGCCCAAGCATTGAAA+CGG + chr3.3:68789259-68789278 MS.gene044280:intron 50.0%
TGACACCGTACTGCCTCTTG+AGG + chr3.3:68788843-68788862 MS.gene044280:CDS 55.0%
TTGTCCAAGTGGGTGGGAAG+TGG + chr3.3:68790581-68790600 MS.gene044280:CDS 55.0%
! CTTGCCACTTCCCACCCACT+TGG - chr3.3:68790588-68790607 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 68788757 68790911 68788757 ID=MS.gene044280
chr3.3 mRNA 68788757 68790911 68788757 ID=MS.gene044280.t1;Parent=MS.gene044280
chr3.3 exon 68788757 68788923 68788757 ID=MS.gene044280.t1.exon1;Parent=MS.gene044280.t1
chr3.3 CDS 68788757 68788923 68788757 ID=cds.MS.gene044280.t1;Parent=MS.gene044280.t1
chr3.3 exon 68789815 68790163 68789815 ID=MS.gene044280.t1.exon2;Parent=MS.gene044280.t1
chr3.3 CDS 68789815 68790163 68789815 ID=cds.MS.gene044280.t1;Parent=MS.gene044280.t1
chr3.3 exon 68790252 68790911 68790252 ID=MS.gene044280.t1.exon3;Parent=MS.gene044280.t1
chr3.3 CDS 68790252 68790911 68790252 ID=cds.MS.gene044280.t1;Parent=MS.gene044280.t1
Gene Sequence

>MS.gene044280

ATGCAAGGAGATAGAAATTTGGAGAAGGTTGAAGGAAGTCTTGCAAAAGCAAGAGCATTGATAAAACAAGCCTTGTTGAGAACCAATGACACCGTACTGCCTCTTGAGGACTCTCATGATTATGTTCCACAAGGAGACATCTACAGAAATGCCTTTGCTTTTCATAGGTAGGATTTTATGAACTATATCATGAGAGATAAGGATTTTACTACTCATAATCCACCTAACGATTGAGTTAGCGCCACTTAAGTAACATAGCAAAGTTTTTTTTTTTTTTTTCTTAAAGAAAGCTAAACAAATCCACCTAATTGATATCAGGAGAATCGAACCTGCAATATCGAAGTTGTATTAGAGTTTACGTTCAAAGGTCTTAGATTTTTTTTGAACAATCAAAAGTGGATTATATATTAACCACAAAAAGCAACTCATCTAGCACAAGATGTGCCAAAAGAATTACCGTAAGAACAAAGAGTCAGTCAAGCAATTAAATACAAACCATTAAGCGATGCCCAAGCATTGAAACGGGCTTGAGCACCACCTATCTGTGCCAAACCTAAAGGCAGCCTTTTTGGCTTTCAACCAGGCTAAAGACATATATTTCACTTTATCTAACAACCTGGGAATAGAAGTTACAACATTATTGAATATGGAGTTGGTCATCCTCATCTTGTGAGCTAACTTTAGTGAGATTCAAATTCATTTAACATAGGTATTGAGCAGTGTAAAATATGATAATGTGAGAATTTAACCCAAGTTTCACGTCATACATGAAAATGAGTATTAAAAATAGTTTCTTAGCTTGGAATGCGCCCATTTAACATTTGTGTTCTCACATTCACACCTCATAACCAAGTGATTGAGCTCAACTGGTTAAACACCTTCATCTTTTGAGCTATCTTTTGGGATGAAATTGAAGCCTATTTAATATGTGGTTGAGATCAAACCATATTTAAGAGTTTTAACCTCTTACATTTTTTTTTGAAGGAAGTTTTAACCTCTTACATGTCATGCTCTATTTTTTCATTCTTTTGAATTGTTTTTACATAATGATGATATAGGAGTTACCACTTAATGGAGAAGCTATTTAAAATTTTTGTGTATGAAGAGGGAGAACCTCCTCTTTTTCACTATGGTCCTTGCAAGAATATATATTCAATGGAGGGAATATTTATCAACTTATTGGAAAGCAATACTTTGTTTCGAACTCAGAATCCAAATGAAGCTCATGTCTACTTTCTTCCATTTAGTGTTGTTATGATCCTTGAACATCTCTTTCACCCAGTTATTCGTGACAAAGCTGTATTGGGAAGAACTATTGGTGATTATGTTCATATCATATCACACAAATATGCATATTGGAATAGAAGTTATGGAGCTGACCATTTCATGCTTTCTTGTCATGATTGGGTACTTATTTTTCAAATTTTCTCCGTTAATTATAGTGTGAGTATGTGACTACTATTTTTATATTTCATGAAAATGGTTTTGTGTATAGGGACCAAGGGCAACATGGTATGTGAAGGAATTGTATTTCATTGCAATTCGAGTACTTTGCAATGCAAATATCTCGGAGCATTTCAATCCAAAGAAAGATGCATCATTTCCTGAAATCAACTTGGTAACAGGAGAAACAACAGGTTTACTTGGTGGCTATCCAACATGGAACAGAACAATTCTGGCTTTCTTTGCAGGACAAATGCATGGTAGAATTAGACCATTGCTTTTCCAACATTGGAAGAACAAAGATAAAGATGTGTTAGTTTATGAAAAACTTCCAGAAAATATTTCATACCATGAAACAATGAAGATGAGTAAATATTGTATTTGTCCAAGTGGGTGGGAAGTGGCAAGTCCAAGAATTGTTGAGGCAATTTATGCTGAATGTGTACCTATTATAATATCACAACAATATGTACTTCCTTTTAGTGATGTTCTTGATTGGGATTCATTTTCTGTTCAAATTGAAGTTAGTGAAATTCCCAAGCTTAAGGAAATTTTGTTGGGTATCTCTGAGGAGAAATATATAAGATTGCAAGAGGGTGTTAAGCAAGTTCAAAGACATTTTGAAGTGAATAATCCTCCTAAGAGATATGATGTATTTCATATGATTATTCATTCTATATGGCTTAGGAGGTTGAATGTACTTGTGAAATAA
Protein sequence

>MS.gene044280.t1

MQGDRNLEKVEGSLAKARALIKQALLRTNDTVLPLEDSHDYVPQGDIYRNAFAFHRSYHLMEKLFKIFVYEEGEPPLFHYGPCKNIYSMEGIFINLLESNTLFRTQNPNEAHVYFLPFSVVMILEHLFHPVIRDKAVLGRTIGDYVHIISHKYAYWNRSYGADHFMLSCHDWGPRATWYVKELYFIAIRVLCNANISEHFNPKKDASFPEINLVTGETTGLLGGYPTWNRTILAFFAGQMHGRIRPLLFQHWKNKDKDVLVYEKLPENISYHETMKMSKYCICPSGWEVASPRIVEAIYAECVPIIISQQYVLPFSDVLDWDSFSVQIEVSEIPKLKEILLGISEEKYIRLQEGVKQVQRHFEVNNPPKRYDVFHMIIHSIWLRRLNVLVK