Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04461.t1 | XP_013460157.1 | 99 | 306 | 1 | 2 | 1 | 305 | 5 | 309 | 4.50E-168 | 600.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04461.t1 | Q8S9H7 | 61.3 | 248 | 76 | 5 | 1 | 245 | 1 | 231 | 2.9e-78 | 293.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04461.t1 | A0A072UYJ1 | 99.0 | 306 | 1 | 2 | 1 | 305 | 5 | 309 | 3.3e-168 | 600.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene04461.t1 | TF | MYB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene04461 | MS.gene036893 | PPI |
| MS.gene04461 | MS.gene038745 | PPI |
| MS.gene060569 | MS.gene04461 | PPI |
| MS.gene060469 | MS.gene04461 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04461.t1 | MTR_3g462790 | 99.020 | 306 | 1 | 2 | 1 | 305 | 5 | 309 | 0.0 | 631 |
| MS.gene04461.t1 | MTR_5g069710 | 66.997 | 303 | 75 | 8 | 1 | 296 | 5 | 289 | 6.90e-137 | 390 |
| MS.gene04461.t1 | MTR_7g067080 | 55.263 | 304 | 104 | 9 | 1 | 292 | 11 | 294 | 4.79e-103 | 305 |
| MS.gene04461.t1 | MTR_5g081860 | 64.975 | 197 | 55 | 4 | 23 | 217 | 16 | 200 | 1.35e-82 | 251 |
| MS.gene04461.t1 | MTR_1g111830 | 45.528 | 246 | 110 | 7 | 37 | 281 | 22 | 244 | 2.74e-59 | 191 |
| MS.gene04461.t1 | MTR_4g111975 | 47.143 | 210 | 74 | 5 | 39 | 217 | 8 | 211 | 2.96e-52 | 174 |
| MS.gene04461.t1 | MTR_8g063600 | 46.561 | 189 | 90 | 2 | 31 | 217 | 2 | 181 | 4.44e-51 | 171 |
| MS.gene04461.t1 | MTR_8g063870 | 41.304 | 184 | 85 | 4 | 36 | 217 | 5 | 167 | 2.30e-40 | 142 |
| MS.gene04461.t1 | MTR_2g100930 | 48.322 | 149 | 59 | 5 | 139 | 270 | 104 | 251 | 4.62e-33 | 125 |
| MS.gene04461.t1 | MTR_4g100630 | 60.000 | 100 | 38 | 1 | 118 | 217 | 71 | 168 | 5.51e-32 | 122 |
| MS.gene04461.t1 | MTR_6g092540 | 73.611 | 72 | 19 | 0 | 138 | 209 | 91 | 162 | 4.37e-31 | 118 |
| MS.gene04461.t1 | MTR_4g015130 | 76.119 | 67 | 16 | 0 | 154 | 220 | 3 | 69 | 1.11e-30 | 111 |
| MS.gene04461.t1 | MTR_8g101650 | 71.233 | 73 | 20 | 1 | 136 | 208 | 97 | 168 | 3.23e-30 | 116 |
| MS.gene04461.t1 | MTR_5g037080 | 68.966 | 87 | 25 | 1 | 131 | 217 | 91 | 175 | 4.16e-30 | 116 |
| MS.gene04461.t1 | MTR_4g107230 | 61.702 | 94 | 32 | 1 | 132 | 225 | 72 | 161 | 1.44e-28 | 112 |
| MS.gene04461.t1 | MTR_5g088010 | 50.980 | 102 | 44 | 3 | 114 | 212 | 32 | 130 | 8.30e-25 | 100 |
| MS.gene04461.t1 | MTR_0036s0260 | 64.179 | 67 | 24 | 0 | 144 | 210 | 28 | 94 | 1.06e-23 | 96.7 |
| MS.gene04461.t1 | MTR_1g033620 | 79.310 | 58 | 12 | 0 | 160 | 217 | 21 | 78 | 1.13e-23 | 97.8 |
| MS.gene04461.t1 | MTR_3g104370 | 58.025 | 81 | 27 | 2 | 141 | 214 | 88 | 168 | 1.94e-23 | 96.7 |
| MS.gene04461.t1 | MTR_1g033600 | 33.333 | 141 | 73 | 2 | 31 | 158 | 4 | 136 | 4.79e-23 | 93.2 |
| MS.gene04461.t1 | MTR_2g090305 | 64.179 | 67 | 24 | 0 | 144 | 210 | 28 | 94 | 5.37e-23 | 94.4 |
| MS.gene04461.t1 | MTR_5g027570 | 61.333 | 75 | 26 | 2 | 140 | 212 | 88 | 161 | 1.18e-22 | 94.7 |
| MS.gene04461.t1 | MTR_5g088060 | 65.714 | 70 | 20 | 2 | 146 | 212 | 274 | 342 | 1.54e-22 | 97.8 |
| MS.gene04461.t1 | MTR_5g488160 | 65.714 | 70 | 20 | 2 | 146 | 212 | 274 | 342 | 1.54e-22 | 97.8 |
| MS.gene04461.t1 | MTR_5g075790 | 35.484 | 186 | 95 | 6 | 16 | 200 | 7 | 168 | 1.69e-22 | 94.0 |
| MS.gene04461.t1 | MTR_5g075760 | 36.000 | 175 | 88 | 5 | 31 | 200 | 5 | 160 | 1.86e-22 | 93.6 |
| MS.gene04461.t1 | MTR_5g027550 | 60.526 | 76 | 27 | 1 | 140 | 212 | 73 | 148 | 1.71e-20 | 88.6 |
| MS.gene04461.t1 | MTR_5g020170 | 50.000 | 68 | 34 | 0 | 34 | 101 | 14 | 81 | 9.91e-20 | 82.8 |
| MS.gene04461.t1 | MTR_3g116720 | 57.576 | 66 | 28 | 0 | 39 | 104 | 16 | 81 | 1.20e-19 | 82.0 |
| MS.gene04461.t1 | MTR_1g022290 | 52.857 | 70 | 33 | 0 | 36 | 105 | 4 | 73 | 2.51e-19 | 81.6 |
| MS.gene04461.t1 | MTR_8g077380 | 52.778 | 72 | 33 | 1 | 36 | 107 | 2 | 72 | 6.53e-19 | 80.1 |
| MS.gene04461.t1 | MTR_8g077390 | 52.778 | 72 | 33 | 1 | 36 | 107 | 2 | 72 | 6.53e-19 | 80.1 |
| MS.gene04461.t1 | MTR_8g077360 | 54.412 | 68 | 30 | 1 | 36 | 103 | 2 | 68 | 6.92e-19 | 79.7 |
| MS.gene04461.t1 | MTR_3g111880 | 48.052 | 77 | 40 | 0 | 36 | 112 | 4 | 80 | 7.44e-19 | 80.5 |
| MS.gene04461.t1 | MTR_1g048660 | 56.250 | 64 | 28 | 0 | 145 | 208 | 13 | 76 | 1.03e-18 | 80.1 |
| MS.gene04461.t1 | MTR_6g004250 | 49.254 | 67 | 34 | 0 | 36 | 102 | 7 | 73 | 6.55e-16 | 72.0 |
| MS.gene04461.t1 | MTR_5g020540 | 55.738 | 61 | 26 | 1 | 39 | 99 | 4 | 63 | 1.58e-15 | 70.5 |
| MS.gene04461.t1 | MTR_8g077420 | 52.174 | 69 | 31 | 2 | 39 | 107 | 5 | 71 | 2.10e-15 | 70.5 |
| MS.gene04461.t1 | MTR_3g013440 | 50.704 | 71 | 34 | 1 | 32 | 101 | 3 | 73 | 1.26e-14 | 68.9 |
| MS.gene04461.t1 | MTR_7g089210 | 52.308 | 65 | 31 | 0 | 43 | 107 | 16 | 80 | 2.04e-14 | 68.2 |
| MS.gene04461.t1 | MTR_3g070110 | 50.667 | 75 | 30 | 1 | 146 | 213 | 97 | 171 | 3.95e-14 | 70.9 |
| MS.gene04461.t1 | MTR_1g083180 | 52.113 | 71 | 29 | 2 | 146 | 212 | 63 | 132 | 1.72e-13 | 68.2 |
| MS.gene04461.t1 | MTR_6g043480 | 53.191 | 47 | 22 | 0 | 144 | 190 | 107 | 153 | 9.69e-12 | 64.3 |
| MS.gene04461.t1 | MTR_3g111920 | 41.667 | 72 | 42 | 0 | 39 | 110 | 3 | 74 | 1.06e-11 | 60.5 |
| MS.gene04461.t1 | MTR_8g072990 | 43.056 | 72 | 36 | 2 | 141 | 208 | 97 | 167 | 1.10e-11 | 64.3 |
| MS.gene04461.t1 | MTR_6g043490 | 53.191 | 47 | 22 | 0 | 144 | 190 | 107 | 153 | 1.63e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04461.t1 | AT5G58900 | 69.500 | 200 | 52 | 3 | 23 | 215 | 15 | 212 | 6.67e-97 | 288 |
| MS.gene04461.t1 | AT2G38090 | 61.947 | 226 | 53 | 5 | 1 | 213 | 5 | 210 | 2.28e-95 | 285 |
| MS.gene04461.t1 | AT5G05790 | 64.062 | 192 | 60 | 4 | 35 | 224 | 27 | 211 | 3.26e-79 | 243 |
| MS.gene04461.t1 | AT5G05790 | 64.062 | 192 | 60 | 4 | 35 | 224 | 27 | 211 | 3.26e-79 | 243 |
| MS.gene04461.t1 | AT3G11280 | 55.000 | 240 | 83 | 6 | 39 | 266 | 33 | 259 | 2.27e-78 | 240 |
| MS.gene04461.t1 | AT3G11280 | 55.000 | 240 | 83 | 6 | 39 | 266 | 33 | 259 | 2.27e-78 | 240 |
| MS.gene04461.t1 | AT5G01200 | 57.843 | 204 | 66 | 5 | 23 | 213 | 19 | 215 | 2.50e-73 | 227 |
| MS.gene04461.t1 | AT5G08520 | 50.256 | 195 | 78 | 3 | 33 | 217 | 6 | 191 | 1.24e-57 | 188 |
| MS.gene04461.t1 | AT1G49010 | 47.716 | 197 | 78 | 3 | 39 | 212 | 8 | 202 | 8.61e-52 | 173 |
| MS.gene04461.t1 | AT5G04760 | 49.162 | 179 | 79 | 3 | 36 | 214 | 3 | 169 | 4.30e-50 | 166 |
| MS.gene04461.t1 | AT5G23650 | 38.866 | 247 | 129 | 5 | 33 | 275 | 7 | 235 | 2.42e-42 | 149 |
| MS.gene04461.t1 | AT5G47390 | 63.529 | 85 | 31 | 0 | 132 | 216 | 84 | 168 | 2.30e-31 | 121 |
| MS.gene04461.t1 | AT3G10580 | 35.714 | 224 | 109 | 8 | 39 | 250 | 8 | 208 | 4.88e-31 | 117 |
| MS.gene04461.t1 | AT3G10580 | 35.714 | 224 | 109 | 8 | 39 | 250 | 8 | 208 | 9.28e-31 | 117 |
| MS.gene04461.t1 | AT5G61620 | 55.140 | 107 | 44 | 1 | 114 | 220 | 82 | 184 | 1.57e-29 | 115 |
| MS.gene04461.t1 | AT4G09450 | 40.116 | 172 | 82 | 3 | 38 | 209 | 6 | 156 | 2.00e-29 | 112 |
| MS.gene04461.t1 | AT5G56840 | 57.609 | 92 | 36 | 2 | 130 | 220 | 79 | 168 | 8.22e-28 | 108 |
| MS.gene04461.t1 | AT3G16350 | 57.547 | 106 | 43 | 1 | 120 | 225 | 113 | 216 | 1.30e-27 | 111 |
| MS.gene04461.t1 | AT1G70000 | 68.354 | 79 | 23 | 1 | 131 | 209 | 87 | 163 | 3.15e-27 | 107 |
| MS.gene04461.t1 | AT1G70000 | 68.354 | 79 | 23 | 1 | 131 | 209 | 87 | 163 | 3.15e-27 | 107 |
| MS.gene04461.t1 | AT1G74840 | 57.692 | 104 | 42 | 1 | 139 | 240 | 91 | 194 | 3.65e-26 | 104 |
| MS.gene04461.t1 | AT1G74840 | 57.692 | 104 | 42 | 1 | 139 | 240 | 91 | 194 | 5.88e-26 | 104 |
| MS.gene04461.t1 | AT1G19000 | 62.791 | 86 | 31 | 1 | 140 | 224 | 98 | 183 | 1.81e-25 | 103 |
| MS.gene04461.t1 | AT1G19000 | 62.791 | 86 | 31 | 1 | 140 | 224 | 98 | 183 | 1.81e-25 | 103 |
| MS.gene04461.t1 | AT1G75250 | 54.667 | 75 | 34 | 0 | 29 | 103 | 2 | 76 | 3.27e-21 | 87.0 |
| MS.gene04461.t1 | AT1G75250 | 54.667 | 75 | 34 | 0 | 29 | 103 | 2 | 76 | 5.79e-21 | 87.0 |
| MS.gene04461.t1 | AT3G10590 | 37.017 | 181 | 98 | 7 | 36 | 209 | 4 | 175 | 2.18e-20 | 87.8 |
| MS.gene04461.t1 | AT2G21650 | 54.545 | 66 | 30 | 0 | 39 | 104 | 14 | 79 | 1.63e-17 | 77.0 |
| MS.gene04461.t1 | AT4G39250 | 46.377 | 69 | 37 | 0 | 39 | 107 | 14 | 82 | 5.52e-16 | 72.8 |
| MS.gene04461.t1 | AT1G19510 | 55.738 | 61 | 27 | 0 | 43 | 103 | 16 | 76 | 1.16e-15 | 72.0 |
| MS.gene04461.t1 | AT2G18328 | 51.613 | 62 | 30 | 0 | 43 | 104 | 15 | 76 | 2.39e-12 | 62.0 |
| MS.gene04461.t1 | AT4G36570 | 61.364 | 44 | 17 | 0 | 39 | 82 | 12 | 55 | 4.08e-11 | 58.2 |
Find 60 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCATGAGTCTTCTTATTT+TGG | 0.078101 | 3.4:+58218235 | MS.gene04461:CDS |
| ATTTGACAGGTTATTTCTAT+TGG | 0.210653 | 3.4:+58217811 | MS.gene04461:intron |
| GGAGTTAATAATAACTCTTA+TGG | 0.241432 | 3.4:+58218175 | MS.gene04461:CDS |
| TATTTCATCAGACAACTTTC+AGG | 0.257079 | 3.4:+58217929 | MS.gene04461:CDS |
| AAACCAATTGTTGGTATTGT+TGG | 0.291167 | 3.4:-58216914 | None:intergenic |
| TTAGATTGGGTGAATTCTTC+TGG | 0.292652 | 3.4:+58217192 | MS.gene04461:CDS |
| TTCACCCAATCTAAGGTAAA+AGG | 0.308169 | 3.4:-58217183 | None:intergenic |
| GCTGGTTTGGTTCCAATTCC+TGG | 0.315843 | 3.4:+58217138 | MS.gene04461:CDS |
| GGTGCATGAGGGTGAGAATT+TGG | 0.327156 | 3.4:-58216877 | None:intergenic |
| CCTTTCTTCCTTTCCTGATC+AGG | 0.333627 | 3.4:-58217267 | None:intergenic |
| TTTGACAGGTTATTTCTATT+GGG | 0.339731 | 3.4:+58217812 | MS.gene04461:intron |
| CTCTTGGTTTCTTCTGAAGT+TGG | 0.347825 | 3.4:-58218025 | None:intergenic |
| AGTGGAAAGAGAGGCTCTTC+TGG | 0.351976 | 3.4:+58217237 | MS.gene04461:CDS |
| ATCTTACTTGTGTTCTTCTT+CGG | 0.354438 | 3.4:-58217301 | None:intergenic |
| GCATTAACAGGAGCACAAAA+TGG | 0.365638 | 3.4:+58216956 | MS.gene04461:CDS |
| AGAAGAATTCACCCAATCTA+AGG | 0.374549 | 3.4:-58217190 | None:intergenic |
| ACATCAACAACACTCTTTCC+AGG | 0.376605 | 3.4:-58217069 | None:intergenic |
| TACTTGTTCATAATCATGTT+TGG | 0.382661 | 3.4:-58218058 | None:intergenic |
| TGCTCTTGCAGTTTATGATA+AGG | 0.387222 | 3.4:+58217008 | MS.gene04461:CDS |
| ATTCCAACAATACCAACAAT+TGG | 0.395051 | 3.4:+58216911 | MS.gene04461:CDS |
| ATTGTAGCAATAGCATTAAC+AGG | 0.398247 | 3.4:+58216944 | MS.gene04461:CDS |
| GATGAATCAGTATAAGGAAT+TGG | 0.401455 | 3.4:+58217092 | MS.gene04461:CDS |
| GTAGTACTATAACCAGGAAT+TGG | 0.407318 | 3.4:-58217150 | None:intergenic |
| GTTTGGTGAAGTTGATCTCT+TGG | 0.418126 | 3.4:-58218041 | None:intergenic |
| TCAAACAATTTGTTCTCTGC+AGG | 0.426400 | 3.4:-58216982 | None:intergenic |
| GTGAATTCTTCTGGTTATGA+TGG | 0.427738 | 3.4:+58217201 | MS.gene04461:CDS |
| TTGTGTTCTTCTTCGGTCCA+TGG | 0.429989 | 3.4:-58217294 | None:intergenic |
| GATGTTTGTAATATAGAAGC+TGG | 0.431912 | 3.4:+58217120 | MS.gene04461:CDS |
| AAAAGTATGGAAAAGGTGAT+TGG | 0.434556 | 3.4:+58217840 | MS.gene04461:CDS |
| TCTGTAACTTCTGATATTGA+AGG | 0.437794 | 3.4:+58218590 | MS.gene04461:CDS |
| TTGTAATATAGAAGCTGGTT+TGG | 0.450084 | 3.4:+58217125 | MS.gene04461:CDS |
| GAGTTATTATTAACTCCATA+AGG | 0.453193 | 3.4:-58218169 | None:intergenic |
| TGATGTGATGAATCAGTATA+AGG | 0.454237 | 3.4:+58217086 | MS.gene04461:CDS |
| TTCTGGTAGATCTCCTGATC+AGG | 0.477520 | 3.4:+58217254 | MS.gene04461:CDS |
| CAAGGAACTTTGTGATCACT+AGG | 0.491493 | 3.4:+58217873 | MS.gene04461:CDS |
| TCTTATGGATTTAAAATGCA+AGG | 0.530791 | 3.4:+58218190 | MS.gene04461:CDS |
| GCTACCTTATGCCACCGATC+CGG | 0.536074 | 3.4:-58217036 | None:intergenic |
| AGAGGAATTAGTGGAAAGAG+AGG | 0.538143 | 3.4:+58217228 | MS.gene04461:CDS |
| CCTGATCAGGAAAGGAAGAA+AGG | 0.540469 | 3.4:+58217267 | MS.gene04461:CDS |
| TTGAATGCTGTCATAGTAGC+TGG | 0.544765 | 3.4:-58218118 | None:intergenic |
| ATGAAAACTTGTTCATGTGA+AGG | 0.546854 | 3.4:-58218142 | None:intergenic |
| AAGGTAGCTGCAATGATCCC+TGG | 0.550717 | 3.4:+58217051 | MS.gene04461:CDS |
| GCATGGCTAGCTACTTGTGT+TGG | 0.551417 | 3.4:-58217899 | None:intergenic |
| TACGCCGGATCGGTGGCATA+AGG | 0.566659 | 3.4:+58217032 | MS.gene04461:CDS |
| CTCAGAAGTGGTGTTAGCAG+TGG | 0.576504 | 3.4:-58218083 | None:intergenic |
| TGATGAAATATTTCTGAGCA+TGG | 0.587728 | 3.4:-58217916 | None:intergenic |
| ATGATAAGGATACGCCGGAT+CGG | 0.588112 | 3.4:+58217022 | MS.gene04461:CDS |
| CTGAAAATGCATCTCAGAAG+TGG | 0.589832 | 3.4:-58218095 | None:intergenic |
| AAATAAGAAGACTCATGAAG+AGG | 0.592849 | 3.4:-58218232 | None:intergenic |
| CATCAACAACACTCTTTCCA+GGG | 0.594776 | 3.4:-58217068 | None:intergenic |
| GTGATTGGAGAAACATATCA+AGG | 0.596845 | 3.4:+58217855 | MS.gene04461:CDS |
| GTAGATCTCCTGATCAGGAA+AGG | 0.600023 | 3.4:+58217259 | MS.gene04461:CDS |
| TTCATCAGACAACTTTCAGG+AGG | 0.604647 | 3.4:+58217932 | MS.gene04461:CDS |
| AAAGGAAGAAAGGTGTGCCA+TGG | 0.605391 | 3.4:+58217277 | MS.gene04461:CDS |
| ATAAGGATACGCCGGATCGG+TGG | 0.605758 | 3.4:+58217025 | MS.gene04461:CDS |
| TGGACCTCAGACACAAAACA+TGG | 0.622490 | 3.4:+58218255 | MS.gene04461:CDS |
| TATGAAAGGTGAAAGCTGCA+AGG | 0.634603 | 3.4:+58218558 | MS.gene04461:intron |
| AGTTTATGATAAGGATACGC+CGG | 0.649185 | 3.4:+58217017 | MS.gene04461:CDS |
| GCAGCAGTAGTACTATAACC+AGG | 0.674867 | 3.4:-58217156 | None:intergenic |
| TTTGAACAAATGGTGCATGA+GGG | 0.682304 | 3.4:-58216888 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTGAGTTATTTATTATTA+TGG | + | chr3.4:58218341-58218360 | MS.gene04461:intron | 10.0% |
| !!! | ATTTATTTAAGTATTTTTGA+GGG | + | chr3.4:58217726-58217745 | MS.gene04461:intron | 10.0% |
| !!! | TATTTATTTAAGTATTTTTG+AGG | + | chr3.4:58217725-58217744 | MS.gene04461:intron | 10.0% |
| !!! | ATATTTATTTAAGTTGTTGA+AGG | + | chr3.4:58217753-58217772 | MS.gene04461:intron | 15.0% |
| !!! | TATTTATTTAAGTTGTTGAA+GGG | + | chr3.4:58217754-58217773 | MS.gene04461:intron | 15.0% |
| !! | CAATATATTTACATAGAACT+TGG | - | chr3.4:58218439-58218458 | None:intergenic | 20.0% |
| !! | CATAAACTTTAAATTACATC+CGG | - | chr3.4:58217586-58217605 | None:intergenic | 20.0% |
| !! | GATTATGAAAATAATTTGAC+AGG | + | chr3.4:58217798-58217817 | MS.gene04461:intron | 20.0% |
| ! | AACTAATATTAGTGCATGTT+TGG | + | chr3.4:58217434-58217453 | MS.gene04461:intron | 25.0% |
| ! | ATCTGTAGTTATTAAGAGTT+AGG | + | chr3.4:58217642-58217661 | MS.gene04461:intron | 25.0% |
| ! | CTTAACTAATAACTACCAAT+AGG | - | chr3.4:58217673-58217692 | None:intergenic | 25.0% |
| ! | GAGTTATTATTAACTCCATA+AGG | - | chr3.4:58218172-58218191 | None:intergenic | 25.0% |
| ! | GGAGTTAATAATAACTCTTA+TGG | + | chr3.4:58218175-58218194 | MS.gene04461:CDS | 25.0% |
| ! | TAAAGTTTATGTATGAACCT+AGG | + | chr3.4:58217595-58217614 | MS.gene04461:intron | 25.0% |
| ! | TACTTGTTCATAATCATGTT+TGG | - | chr3.4:58218061-58218080 | None:intergenic | 25.0% |
| !! | TAGAACAAGTATCTTTTGTT+TGG | + | chr3.4:58218365-58218384 | MS.gene04461:intron | 25.0% |
| !! | TATGTGAAGATTTTACTGTT+TGG | + | chr3.4:58217384-58217403 | MS.gene04461:intron | 25.0% |
| !! | TCTTATGGATTTAAAATGCA+AGG | + | chr3.4:58218190-58218209 | MS.gene04461:CDS | 25.0% |
| !! | TGACAAAAGCTACATTTTAA+AGG | - | chr3.4:58217496-58217515 | None:intergenic | 25.0% |
| !! | TTGTTGGAATTTTGAACAAA+TGG | - | chr3.4:58216901-58216920 | None:intergenic | 25.0% |
| !!! | ATTTGACAGGTTATTTCTAT+TGG | + | chr3.4:58217811-58217830 | MS.gene04461:intron | 25.0% |
| !!! | TTTGACAGGTTATTTCTATT+GGG | + | chr3.4:58217812-58217831 | MS.gene04461:intron | 25.0% |
| AAACCAATTGTTGGTATTGT+TGG | - | chr3.4:58216917-58216936 | None:intergenic | 30.0% | |
| AAATAAGAAGACTCATGAAG+AGG | - | chr3.4:58218235-58218254 | None:intergenic | 30.0% | |
| AACAACCTTAAACCTATGAA+AGG | + | chr3.4:58218544-58218563 | MS.gene04461:intron | 30.0% | |
| AAGAGTTAGGTATTTCCTAT+TGG | + | chr3.4:58217655-58217674 | MS.gene04461:intron | 30.0% | |
| ATCAAGTTCATATACCAAGA+AGG | - | chr3.4:58218492-58218511 | None:intergenic | 30.0% | |
| ATCTTACTTGTGTTCTTCTT+CGG | - | chr3.4:58217304-58217323 | None:intergenic | 30.0% | |
| ATCTTCCAAAAACCAATTGT+TGG | - | chr3.4:58216926-58216945 | None:intergenic | 30.0% | |
| ATTCCAACAATACCAACAAT+TGG | + | chr3.4:58216911-58216930 | MS.gene04461:CDS | 30.0% | |
| ATTGTAGCAATAGCATTAAC+AGG | + | chr3.4:58216944-58216963 | MS.gene04461:CDS | 30.0% | |
| GATGAATCAGTATAAGGAAT+TGG | + | chr3.4:58217092-58217111 | MS.gene04461:CDS | 30.0% | |
| GATGTTTGTAATATAGAAGC+TGG | + | chr3.4:58217120-58217139 | MS.gene04461:CDS | 30.0% | |
| TATTTCATCAGACAACTTTC+AGG | + | chr3.4:58217929-58217948 | MS.gene04461:CDS | 30.0% | |
| TCTGTAACTTCTGATATTGA+AGG | + | chr3.4:58218590-58218609 | MS.gene04461:CDS | 30.0% | |
| TGATGTGATGAATCAGTATA+AGG | + | chr3.4:58217086-58217105 | MS.gene04461:CDS | 30.0% | |
| TTAGTTAGTTATTGCCTTCT+TGG | + | chr3.4:58218475-58218494 | MS.gene04461:intron | 30.0% | |
| TTGTAATATAGAAGCTGGTT+TGG | + | chr3.4:58217125-58217144 | MS.gene04461:CDS | 30.0% | |
| TTTCACCTTTCATAGGTTTA+AGG | - | chr3.4:58218552-58218571 | None:intergenic | 30.0% | |
| ! | AAAAGTATGGAAAAGGTGAT+TGG | + | chr3.4:58217840-58217859 | MS.gene04461:CDS | 30.0% |
| ! | ATGAAAACTTGTTCATGTGA+AGG | - | chr3.4:58218145-58218164 | None:intergenic | 30.0% |
| ! | TATGAACTTGATGATAGCAT+TGG | + | chr3.4:58218500-58218519 | MS.gene04461:intron | 30.0% |
| ! | TGATGAAATATTTCTGAGCA+TGG | - | chr3.4:58217919-58217938 | None:intergenic | 30.0% |
| ! | TTCACCTTTTACCTTAGATT+GGG | + | chr3.4:58217179-58217198 | MS.gene04461:CDS | 30.0% |
| ! | TTTTGTCAAAATCACGATGA+CGG | + | chr3.4:58217508-58217527 | MS.gene04461:intron | 30.0% |
| !! | CTTCATGAGTCTTCTTATTT+TGG | + | chr3.4:58218235-58218254 | MS.gene04461:CDS | 30.0% |
| !!! | CAATACCAACAATTGGTTTT+TGG | + | chr3.4:58216918-58216937 | MS.gene04461:CDS | 30.0% |
| ACTTTAAATTACATCCGGTC+AGG | - | chr3.4:58217581-58217600 | None:intergenic | 35.0% | |
| AGAAGAATTCACCCAATCTA+AGG | - | chr3.4:58217193-58217212 | None:intergenic | 35.0% | |
| AGTTTATGATAAGGATACGC+CGG | + | chr3.4:58217017-58217036 | MS.gene04461:CDS | 35.0% | |
| ATTCACATGCATGATGTTGT+TGG | + | chr3.4:58218308-58218327 | MS.gene04461:intron | 35.0% | |
| CTATTGGGTCTGAAAAAGTA+TGG | + | chr3.4:58217827-58217846 | MS.gene04461:CDS | 35.0% | |
| GTAGTACTATAACCAGGAAT+TGG | - | chr3.4:58217153-58217172 | None:intergenic | 35.0% | |
| GTGATTGGAGAAACATATCA+AGG | + | chr3.4:58217855-58217874 | MS.gene04461:CDS | 35.0% | |
| GTTTGGATTGACGGTTAATT+TGG | - | chr3.4:58217553-58217572 | None:intergenic | 35.0% | |
| TGCTCTTGCAGTTTATGATA+AGG | + | chr3.4:58217008-58217027 | MS.gene04461:CDS | 35.0% | |
| TTAGATTGGGTGAATTCTTC+TGG | + | chr3.4:58217192-58217211 | MS.gene04461:CDS | 35.0% | |
| TTATGTATGAACCTAGGAGT+TGG | + | chr3.4:58217601-58217620 | MS.gene04461:intron | 35.0% | |
| TTTGAACAAATGGTGCATGA+GGG | - | chr3.4:58216891-58216910 | None:intergenic | 35.0% | |
| ! | AAAACCATGTTTTGTGTCTG+AGG | - | chr3.4:58218262-58218281 | None:intergenic | 35.0% |
| ! | CAAGTTTTCATGTCTCCTTA+TGG | + | chr3.4:58218154-58218173 | MS.gene04461:CDS | 35.0% |
| ! | CTTCACCTTTTACCTTAGAT+TGG | + | chr3.4:58217178-58217197 | MS.gene04461:CDS | 35.0% |
| ! | GGTCTGAAAAAGTATGGAAA+AGG | + | chr3.4:58217833-58217852 | MS.gene04461:CDS | 35.0% |
| ! | GTGAATTCTTCTGGTTATGA+TGG | + | chr3.4:58217201-58217220 | MS.gene04461:CDS | 35.0% |
| ! | TCAAACAATTTGTTCTCTGC+AGG | - | chr3.4:58216985-58217004 | None:intergenic | 35.0% |
| ! | TTCACCCAATCTAAGGTAAA+AGG | - | chr3.4:58217186-58217205 | None:intergenic | 35.0% |
| ! | TTTTGAACAAATGGTGCATG+AGG | - | chr3.4:58216892-58216911 | None:intergenic | 35.0% |
| !!! | GATGGTTTTAGAGGAATTAG+TGG | + | chr3.4:58217219-58217238 | MS.gene04461:CDS | 35.0% |
| !!! | TCTGGTTATGATGGTTTTAG+AGG | + | chr3.4:58217210-58217229 | MS.gene04461:CDS | 35.0% |
| ACATCAACAACACTCTTTCC+AGG | - | chr3.4:58217072-58217091 | None:intergenic | 40.0% | |
| AGAGGAATTAGTGGAAAGAG+AGG | + | chr3.4:58217228-58217247 | MS.gene04461:CDS | 40.0% | |
| ATTTCACTTCACCAACTCCT+AGG | - | chr3.4:58217615-58217634 | None:intergenic | 40.0% | |
| CAAGGAACTTTGTGATCACT+AGG | + | chr3.4:58217873-58217892 | MS.gene04461:CDS | 40.0% | |
| CATCAACAACACTCTTTCCA+GGG | - | chr3.4:58217071-58217090 | None:intergenic | 40.0% | |
| CTGAAAATGCATCTCAGAAG+TGG | - | chr3.4:58218098-58218117 | None:intergenic | 40.0% | |
| GTTGTTGAGAAGATTGCATC+TGG | - | chr3.4:58218285-58218304 | None:intergenic | 40.0% | |
| TATGAAAGGTGAAAGCTGCA+AGG | + | chr3.4:58218558-58218577 | MS.gene04461:intron | 40.0% | |
| TTCATCAGACAACTTTCAGG+AGG | + | chr3.4:58217932-58217951 | MS.gene04461:CDS | 40.0% | |
| TTGAATGCTGTCATAGTAGC+TGG | - | chr3.4:58218121-58218140 | None:intergenic | 40.0% | |
| TTGCAGCTTTCACCTTTCAT+AGG | - | chr3.4:58218559-58218578 | None:intergenic | 40.0% | |
| ! | GCATTAACAGGAGCACAAAA+TGG | + | chr3.4:58216956-58216975 | MS.gene04461:CDS | 40.0% |
| ! | GTTTGGTGAAGTTGATCTCT+TGG | - | chr3.4:58218044-58218063 | None:intergenic | 40.0% |
| !! | CTCTTGGTTTCTTCTGAAGT+TGG | - | chr3.4:58218028-58218047 | None:intergenic | 40.0% |
| !! | TGCATGATGTTGTTGGTGAT+TGG | + | chr3.4:58218315-58218334 | MS.gene04461:intron | 40.0% |
| AATCCAAACATGCACCTGAC+CGG | + | chr3.4:58217564-58217583 | MS.gene04461:intron | 45.0% | |
| ATGATAAGGATACGCCGGAT+CGG | + | chr3.4:58217022-58217041 | MS.gene04461:CDS | 45.0% | |
| CAGGTGCATGTTTGGATTGA+CGG | - | chr3.4:58217562-58217581 | None:intergenic | 45.0% | |
| CCTGATCAGGAAAGGAAGAA+AGG | + | chr3.4:58217267-58217286 | MS.gene04461:CDS | 45.0% | |
| CCTTTCTTCCTTTCCTGATC+AGG | - | chr3.4:58217270-58217289 | None:intergenic | 45.0% | |
| GCAGCAGTAGTACTATAACC+AGG | - | chr3.4:58217159-58217178 | None:intergenic | 45.0% | |
| GTAGATCTCCTGATCAGGAA+AGG | + | chr3.4:58217259-58217278 | MS.gene04461:CDS | 45.0% | |
| TGGACCTCAGACACAAAACA+TGG | + | chr3.4:58218255-58218274 | MS.gene04461:CDS | 45.0% | |
| TTCTGGTAGATCTCCTGATC+AGG | + | chr3.4:58217254-58217273 | MS.gene04461:CDS | 45.0% | |
| TTGTGTTCTTCTTCGGTCCA+TGG | - | chr3.4:58217297-58217316 | None:intergenic | 45.0% | |
| ! | AAAGGAAGAAAGGTGTGCCA+TGG | + | chr3.4:58217277-58217296 | MS.gene04461:CDS | 45.0% |
| AAGGTAGCTGCAATGATCCC+TGG | + | chr3.4:58217051-58217070 | MS.gene04461:CDS | 50.0% | |
| AGTGGAAAGAGAGGCTCTTC+TGG | + | chr3.4:58217237-58217256 | MS.gene04461:CDS | 50.0% | |
| GGTGCATGAGGGTGAGAATT+TGG | - | chr3.4:58216880-58216899 | None:intergenic | 50.0% | |
| ! | CTCAGAAGTGGTGTTAGCAG+TGG | - | chr3.4:58218086-58218105 | None:intergenic | 50.0% |
| ! | GCATGGCTAGCTACTTGTGT+TGG | - | chr3.4:58217902-58217921 | None:intergenic | 50.0% |
| !! | GCTGGTTTGGTTCCAATTCC+TGG | + | chr3.4:58217138-58217157 | MS.gene04461:CDS | 50.0% |
| ATAAGGATACGCCGGATCGG+TGG | + | chr3.4:58217025-58217044 | MS.gene04461:CDS | 55.0% | |
| CATCCGGTCAGGTGCATGTT+TGG | - | chr3.4:58217570-58217589 | None:intergenic | 55.0% | |
| GCTACCTTATGCCACCGATC+CGG | - | chr3.4:58217039-58217058 | None:intergenic | 55.0% | |
| TACGCCGGATCGGTGGCATA+AGG | + | chr3.4:58217032-58217051 | MS.gene04461:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 58216862 | 58218619 | 58216862 | ID=MS.gene04461 |
| chr3.4 | mRNA | 58216862 | 58218619 | 58216862 | ID=MS.gene04461.t1;Parent=MS.gene04461 |
| chr3.4 | exon | 58216862 | 58217316 | 58216862 | ID=MS.gene04461.t1.exon1;Parent=MS.gene04461.t1 |
| chr3.4 | CDS | 58216862 | 58217316 | 58216862 | ID=cds.MS.gene04461.t1;Parent=MS.gene04461.t1 |
| chr3.4 | exon | 58217820 | 58218276 | 58217820 | ID=MS.gene04461.t1.exon2;Parent=MS.gene04461.t1 |
| chr3.4 | CDS | 58217820 | 58218276 | 58217820 | ID=cds.MS.gene04461.t1;Parent=MS.gene04461.t1 |
| chr3.4 | exon | 58218566 | 58218619 | 58218566 | ID=MS.gene04461.t1.exon3;Parent=MS.gene04461.t1 |
| chr3.4 | CDS | 58218566 | 58218619 | 58218566 | ID=cds.MS.gene04461.t1;Parent=MS.gene04461.t1 |
| Gene Sequence |
| Protein sequence |
