Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044943.t1 | XP_003621904.1 | 92.7 | 562 | 23 | 1 | 1 | 544 | 1 | 562 | 3.90E-289 | 1003.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044943.t1 | Q9MAU3 | 60.7 | 550 | 209 | 3 | 1 | 544 | 1 | 549 | 8.9e-181 | 634.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044943.t1 | G7KXV7 | 92.7 | 562 | 23 | 1 | 1 | 544 | 1 | 562 | 2.8e-289 | 1003.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044943 | MS.gene26816 | PPI |
| MS.gene044943 | MS.gene030125 | PPI |
| MS.gene044943 | MS.gene061229 | PPI |
| MS.gene044943 | MS.gene68598 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044943.t1 | MTR_7g024860 | 94.484 | 562 | 13 | 1 | 1 | 544 | 1 | 562 | 0.0 | 1083 |
| MS.gene044943.t1 | MTR_7g024867 | 94.484 | 562 | 13 | 1 | 1 | 544 | 1 | 562 | 0.0 | 1083 |
| MS.gene044943.t1 | MTR_3g055240 | 57.721 | 544 | 196 | 5 | 1 | 544 | 1 | 510 | 0.0 | 595 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044943.t1 | AT1G04950 | 63.177 | 554 | 189 | 6 | 1 | 544 | 1 | 549 | 0.0 | 683 |
| MS.gene044943.t1 | AT1G04950 | 63.177 | 554 | 189 | 6 | 1 | 544 | 1 | 549 | 0.0 | 683 |
| MS.gene044943.t1 | AT1G04950 | 63.177 | 554 | 189 | 6 | 1 | 544 | 1 | 549 | 0.0 | 683 |
| MS.gene044943.t1 | AT1G54360 | 46.881 | 561 | 244 | 7 | 3 | 544 | 15 | 540 | 7.52e-160 | 466 |
| MS.gene044943.t1 | AT1G54360 | 46.881 | 561 | 244 | 7 | 3 | 544 | 1 | 526 | 8.07e-160 | 466 |
Find 107 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGTTCATCTTTCCAAATA+TGG | 0.136550 | 7.1:+73020431 | None:intergenic |
| CATGAAGCTTGGCGTGTTTA+TGG | 0.154260 | 7.1:-73021623 | MS.gene044943:CDS |
| CTTCTGTCCTAGCCCATATT+TGG | 0.203384 | 7.1:-73020444 | MS.gene044943:CDS |
| TTCTTATGTCAGCCAATATA+TGG | 0.220354 | 7.1:-73024909 | MS.gene044943:intron |
| AACGTTGCTGAATGCATTCT+TGG | 0.247007 | 7.1:-73022531 | MS.gene044943:CDS |
| GTAAGTTTGGCTACTGATTC+AGG | 0.252357 | 7.1:-73023965 | MS.gene044943:CDS |
| TGTTGTGTCATTGCCTTCTT+TGG | 0.257279 | 7.1:+73022506 | None:intergenic |
| CGTATATATGCTTCAAGATT+TGG | 0.258387 | 7.1:+73021698 | None:intergenic |
| TAAAGAAGCATTAGTAAGTT+TGG | 0.260790 | 7.1:-73023978 | MS.gene044943:CDS |
| TAACCTGCCTTGTTGCTAAA+AGG | 0.298000 | 7.1:-73023319 | MS.gene044943:CDS |
| ATAACCTCGATTGTTTCTTT+AGG | 0.299682 | 7.1:+73027441 | None:intergenic |
| CTAGATAAGACATGCTTAAC+AGG | 0.311507 | 7.1:+73024148 | None:intergenic |
| GCTACAAAGCATGTGAAATA+AGG | 0.321309 | 7.1:+73023926 | None:intergenic |
| TGGAAAGATGAACTGAATTC+AGG | 0.326248 | 7.1:-73020424 | MS.gene044943:CDS |
| AGGTTATTGCTCAGAGTATT+GGG | 0.333702 | 7.1:-73027425 | MS.gene044943:CDS |
| AACTTACATAAGGTTCAATA+TGG | 0.335073 | 7.1:+73023721 | None:intergenic |
| ACAACACTATGGTGCAATTC+AGG | 0.341769 | 7.1:-73022489 | MS.gene044943:CDS |
| CAGTTCATCTTTCCAAATAT+GGG | 0.342840 | 7.1:+73020432 | None:intergenic |
| CAAGGCAAGCCCTATGGAAT+TGG | 0.343736 | 7.1:-73020686 | MS.gene044943:CDS |
| TGTCTTATCTAGAGAGCTTC+AGG | 0.353561 | 7.1:-73024137 | MS.gene044943:intron |
| TCTTTAAAGAGAACCGAATC+AGG | 0.355917 | 7.1:+73023995 | None:intergenic |
| ACAGGTAATTTGATATCAAC+TGG | 0.361767 | 7.1:+73024166 | None:intergenic |
| CCAGATGCAAATCCATATAT+TGG | 0.371634 | 7.1:+73024897 | None:intergenic |
| GCGTGTTTATGGAGCCTTGC+TGG | 0.371996 | 7.1:-73021612 | MS.gene044943:intron |
| GGGAGAGGAGATGGAAAAGC+GGG | 0.385964 | 7.1:+73021446 | None:intergenic |
| CCTGAAAATGCTCCTGTAGA+TGG | 0.391023 | 7.1:-73024621 | MS.gene044943:intron |
| ACATCATTGGTTGAGCTGTT+TGG | 0.393677 | 7.1:-73020391 | MS.gene044943:CDS |
| TGGAGATGTTAGTGTAGTTC+AGG | 0.394478 | 7.1:-73020629 | MS.gene044943:CDS |
| TGTGTCTCAAGAAGCATCTC+TGG | 0.409556 | 7.1:+73021665 | None:intergenic |
| TGCCTTGTTGCTAAAAGGTT+GGG | 0.413976 | 7.1:-73023314 | MS.gene044943:CDS |
| GAGGTTATTGCTCAGAGTAT+TGG | 0.414899 | 7.1:-73027426 | MS.gene044943:CDS |
| GGCTGCTGCTCCAATTCCAT+AGG | 0.419772 | 7.1:+73020676 | None:intergenic |
| CTCCTCTCCCTCATACTGTC+TGG | 0.432250 | 7.1:-73021433 | MS.gene044943:CDS |
| ACAAGGCAGGTTACAATTGA+TGG | 0.435760 | 7.1:+73023329 | None:intergenic |
| AGCTTTGAATGAGCCTGATT+CGG | 0.436229 | 7.1:-73024008 | MS.gene044943:CDS |
| GCAGTATCAAGGGGAGCTTT+TGG | 0.439198 | 7.1:+73024690 | None:intergenic |
| CAGTATCTACTTTCTTCAAA+GGG | 0.439281 | 7.1:+73020653 | None:intergenic |
| TGTTTCATTCAGCGTGCTGC+AGG | 0.440328 | 7.1:-73021500 | MS.gene044943:intron |
| CCAATATATGGATTTGCATC+TGG | 0.443044 | 7.1:-73024897 | MS.gene044943:CDS |
| CTGCCTTGTTGCTAAAAGGT+TGG | 0.459360 | 7.1:-73023315 | MS.gene044943:CDS |
| ACAGTATGAGGGAGAGGAGA+TGG | 0.459983 | 7.1:+73021437 | None:intergenic |
| GCAACAGAAAGCGCAACATC+AGG | 0.464025 | 7.1:+73027387 | None:intergenic |
| TATGGTGCAATTCAGGGGTT+AGG | 0.464972 | 7.1:-73022482 | MS.gene044943:CDS |
| CATCTTTCCAAATATGGGCT+AGG | 0.469577 | 7.1:+73020437 | None:intergenic |
| ATTGGTGCAGCGTCATCCTC+TGG | 0.470899 | 7.1:-73020541 | MS.gene044943:CDS |
| CTGGTCTTCCAGACAGTATG+AGG | 0.477347 | 7.1:+73021425 | None:intergenic |
| CAAGATTTGGCAGCAAAAGA+AGG | 0.477595 | 7.1:+73021711 | None:intergenic |
| AGGACGTGTCCTGACATCAT+TGG | 0.478074 | 7.1:-73020404 | MS.gene044943:CDS |
| CGCATACGGTATTCAACATC+AGG | 0.480045 | 7.1:+73027363 | None:intergenic |
| GACAGGTAAGTGCAGTATCA+AGG | 0.484319 | 7.1:+73024679 | None:intergenic |
| TCAGTATCTACTTTCTTCAA+AGG | 0.486930 | 7.1:+73020652 | None:intergenic |
| ATACTGCACTTACCTGTCAC+TGG | 0.488327 | 7.1:-73024674 | MS.gene044943:CDS |
| TTCCAGACAGTATGAGGGAG+AGG | 0.490867 | 7.1:+73021431 | None:intergenic |
| ACAGGTAAGTGCAGTATCAA+GGG | 0.492623 | 7.1:+73024680 | None:intergenic |
| GTCTCCTCTTCCTTATGAGA+TGG | 0.492865 | 7.1:+73020595 | None:intergenic |
| AAATTCTTCACCATCTCATA+AGG | 0.493204 | 7.1:-73020605 | MS.gene044943:CDS |
| TTACAATTGATGGCATCAAC+TGG | 0.493457 | 7.1:+73023339 | None:intergenic |
| TTCGATGGCAAGCCAGTGAC+AGG | 0.496154 | 7.1:+73024662 | None:intergenic |
| TAAACGCAAGGCAAGCCCTA+TGG | 0.498294 | 7.1:-73020692 | MS.gene044943:CDS |
| AAGGCAATGACACAACACTA+TGG | 0.502589 | 7.1:-73022500 | MS.gene044943:CDS |
| TTGGGAACTTAGAGACTTCA+CGG | 0.503314 | 7.1:-73023270 | MS.gene044943:CDS |
| CAATCCTTTCAGGTTTCTCG+TGG | 0.508236 | 7.1:-73023813 | MS.gene044943:intron |
| AGGGAGAGGAGATGGAAAAG+CGG | 0.510840 | 7.1:+73021445 | None:intergenic |
| CAACACTATGGTGCAATTCA+GGG | 0.511881 | 7.1:-73022488 | MS.gene044943:CDS |
| GGTGAAGTTAGAAGCAGTGA+GGG | 0.512556 | 7.1:-73020490 | MS.gene044943:CDS |
| TGCATTCTTGGATCCAAAGA+AGG | 0.513153 | 7.1:-73022519 | MS.gene044943:CDS |
| GAGAGGGACAATACCACATT+GGG | 0.513459 | 7.1:+73022449 | None:intergenic |
| GTTAGATAGCTACCATCTAC+AGG | 0.515613 | 7.1:+73024609 | None:intergenic |
| CACATGCTTTGTAGCTGATG+AGG | 0.521153 | 7.1:-73023918 | MS.gene044943:intron |
| GCTGCTGCTCCAATTCCATA+GGG | 0.522208 | 7.1:+73020677 | None:intergenic |
| GCTATTAACAACTCGCATCA+AGG | 0.536109 | 7.1:+73023764 | None:intergenic |
| TGATTCAGGACTTCATCCAT+TGG | 0.539715 | 7.1:-73023951 | MS.gene044943:CDS |
| TGGTTGCATCAATATGCAAA+AGG | 0.541657 | 7.1:-73023241 | MS.gene044943:intron |
| TAGAGACTTCACGGCTAACC+TGG | 0.544251 | 7.1:-73023261 | MS.gene044943:CDS |
| CATGTGAAATAAGGAACCAA+TGG | 0.544256 | 7.1:+73023935 | None:intergenic |
| CCTGCATGATCTGACGCATA+CGG | 0.547929 | 7.1:+73027349 | None:intergenic |
| CGCATCAAGGCAAATAGAAG+TGG | 0.550878 | 7.1:+73023777 | None:intergenic |
| TTTATAGCAATCTACAGTCC+CGG | 0.553507 | 7.1:-73022562 | MS.gene044943:CDS |
| AAATGAAAAGACATGAAGCT+TGG | 0.554764 | 7.1:-73021634 | MS.gene044943:CDS |
| TTCACCATCTCATAAGGAAG+AGG | 0.558979 | 7.1:-73020599 | MS.gene044943:CDS |
| ACAGCTCAACCAATGATGTC+AGG | 0.559937 | 7.1:+73020395 | None:intergenic |
| TTGAAGAAAGTAGATACTGA+TGG | 0.560684 | 7.1:-73020649 | MS.gene044943:CDS |
| TAGATTGACAGACAACCATT+GGG | 0.562642 | 7.1:-73023288 | MS.gene044943:CDS |
| GTAGATTGACAGACAACCAT+TGG | 0.564706 | 7.1:-73023289 | MS.gene044943:CDS |
| AAGCAGTGAGGGTGATACTC+AGG | 0.570545 | 7.1:-73020479 | MS.gene044943:CDS |
| AGCAGGTTGCACTCCTTCGA+TGG | 0.571796 | 7.1:+73024647 | None:intergenic |
| TGTCACTGGCTTGCCATCGA+AGG | 0.581402 | 7.1:-73024660 | MS.gene044943:CDS |
| ATTCAGGGGTTAGGAGCACT+AGG | 0.597296 | 7.1:-73022473 | MS.gene044943:CDS |
| AGAAGTAAGAACACTTGCAC+TGG | 0.600577 | 7.1:+73021406 | None:intergenic |
| GGAGAGGGACAATACCACAT+TGG | 0.615478 | 7.1:+73022448 | None:intergenic |
| CCGTATGCGTCAGATCATGC+AGG | 0.616047 | 7.1:-73027349 | MS.gene044943:intron |
| AAACAAATCTCTGTGTCCGA+CGG | 0.619921 | 7.1:+73024851 | None:intergenic |
| TGATCATGAAGATAAACGCA+AGG | 0.624991 | 7.1:-73020704 | MS.gene044943:intron |
| AACACTATGGTGCAATTCAG+GGG | 0.625157 | 7.1:-73022487 | MS.gene044943:CDS |
| AGTATCTACTTTCTTCAAAG+GGG | 0.630790 | 7.1:+73020654 | None:intergenic |
| GTATCTACTTTCTTCAAAGG+GGG | 0.631481 | 7.1:+73020655 | None:intergenic |
| ATATATGGATTTGCATCTGG+TGG | 0.632207 | 7.1:-73024894 | MS.gene044943:CDS |
| GATAACATATCTTACCAGCA+AGG | 0.646319 | 7.1:+73021598 | None:intergenic |
| TGAAGTCTCTAAGTTCCCAA+TGG | 0.651064 | 7.1:+73023273 | None:intergenic |
| CAGGTAAGTGCAGTATCAAG+GGG | 0.654159 | 7.1:+73024681 | None:intergenic |
| CTACGATTCAAACGAGCCGT+CGG | 0.663596 | 7.1:-73024867 | MS.gene044943:CDS |
| TGGTCTTCCAGACAGTATGA+GGG | 0.668975 | 7.1:+73021426 | None:intergenic |
| ACGGCTCGTTTGAATCGTAG+AGG | 0.675689 | 7.1:+73024870 | None:intergenic |
| TGGTGAAGTTAGAAGCAGTG+AGG | 0.680296 | 7.1:-73020491 | MS.gene044943:CDS |
| TGTTCCTAAAGAAACAATCG+AGG | 0.685796 | 7.1:-73027445 | MS.gene044943:CDS |
| AGGAGCACTAGGACCCAATG+TGG | 0.719832 | 7.1:-73022462 | MS.gene044943:intron |
| GGAGAGGAGATGGAAAAGCG+GGG | 0.719916 | 7.1:+73021447 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 73020339 | 73027475 | 73020339 | ID=MS.gene044943 |
| chr7.1 | mRNA | 73020339 | 73027475 | 73020339 | ID=MS.gene044943.t1;Parent=MS.gene044943 |
| chr7.1 | exon | 73027350 | 73027475 | 73027350 | ID=MS.gene044943.t1.exon1;Parent=MS.gene044943.t1 |
| chr7.1 | CDS | 73027350 | 73027475 | 73027350 | ID=cds.MS.gene044943.t1;Parent=MS.gene044943.t1 |
| chr7.1 | exon | 73025848 | 73025934 | 73025848 | ID=MS.gene044943.t1.exon2;Parent=MS.gene044943.t1 |
| chr7.1 | CDS | 73025848 | 73025934 | 73025848 | ID=cds.MS.gene044943.t1;Parent=MS.gene044943.t1 |
| chr7.1 | exon | 73024818 | 73024919 | 73024818 | ID=MS.gene044943.t1.exon3;Parent=MS.gene044943.t1 |
| chr7.1 | CDS | 73024818 | 73024919 | 73024818 | ID=cds.MS.gene044943.t1;Parent=MS.gene044943.t1 |
| chr7.1 | exon | 73024622 | 73024730 | 73024622 | ID=MS.gene044943.t1.exon4;Parent=MS.gene044943.t1 |
| chr7.1 | CDS | 73024622 | 73024730 | 73024622 | ID=cds.MS.gene044943.t1;Parent=MS.gene044943.t1 |
| chr7.1 | exon | 73024138 | 73024241 | 73024138 | ID=MS.gene044943.t1.exon5;Parent=MS.gene044943.t1 |
| chr7.1 | CDS | 73024138 | 73024241 | 73024138 | ID=cds.MS.gene044943.t1;Parent=MS.gene044943.t1 |
| chr7.1 | exon | 73023919 | 73024056 | 73023919 | ID=MS.gene044943.t1.exon6;Parent=MS.gene044943.t1 |
| chr7.1 | CDS | 73023919 | 73024056 | 73023919 | ID=cds.MS.gene044943.t1;Parent=MS.gene044943.t1 |
| chr7.1 | exon | 73023728 | 73023823 | 73023728 | ID=MS.gene044943.t1.exon7;Parent=MS.gene044943.t1 |
| chr7.1 | CDS | 73023728 | 73023823 | 73023728 | ID=cds.MS.gene044943.t1;Parent=MS.gene044943.t1 |
| chr7.1 | exon | 73023242 | 73023366 | 73023242 | ID=MS.gene044943.t1.exon8;Parent=MS.gene044943.t1 |
| chr7.1 | CDS | 73023242 | 73023366 | 73023242 | ID=cds.MS.gene044943.t1;Parent=MS.gene044943.t1 |
| chr7.1 | exon | 73022463 | 73022595 | 73022463 | ID=MS.gene044943.t1.exon9;Parent=MS.gene044943.t1 |
| chr7.1 | CDS | 73022463 | 73022595 | 73022463 | ID=cds.MS.gene044943.t1;Parent=MS.gene044943.t1 |
| chr7.1 | exon | 73021613 | 73021738 | 73021613 | ID=MS.gene044943.t1.exon10;Parent=MS.gene044943.t1 |
| chr7.1 | CDS | 73021613 | 73021738 | 73021613 | ID=cds.MS.gene044943.t1;Parent=MS.gene044943.t1 |
| chr7.1 | exon | 73021399 | 73021510 | 73021399 | ID=MS.gene044943.t1.exon11;Parent=MS.gene044943.t1 |
| chr7.1 | CDS | 73021399 | 73021510 | 73021399 | ID=cds.MS.gene044943.t1;Parent=MS.gene044943.t1 |
| chr7.1 | exon | 73020339 | 73020715 | 73020339 | ID=MS.gene044943.t1.exon12;Parent=MS.gene044943.t1 |
| chr7.1 | CDS | 73020339 | 73020715 | 73020339 | ID=cds.MS.gene044943.t1;Parent=MS.gene044943.t1 |
| Gene Sequence |
| Protein sequence |
