AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene045389


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045389.t1 MTR_3g083760 82.673 202 16 1 1 183 1 202 1.18e-94 273
MS.gene045389.t1 MTR_3g083760 87.413 143 16 1 1 141 1 143 6.48e-81 236
MS.gene045389.t1 MTR_8g085560 50.829 181 83 3 9 183 14 194 5.45e-66 201
MS.gene045389.t1 MTR_7g014880 42.222 180 93 3 8 176 12 191 3.99e-41 137
MS.gene045389.t1 MTR_8g446830 39.487 195 105 6 1 182 1 195 1.05e-36 126
MS.gene045389.t1 MTR_1g103450 31.333 150 79 4 12 161 15 140 7.10e-13 63.5
MS.gene045389.t1 MTR_1g103490 36.667 90 55 2 12 101 10 97 2.34e-12 62.0
MS.gene045389.t1 MTR_1g103470 39.189 74 42 2 28 101 25 95 1.66e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045389.t1 AT1G55260 43.258 178 93 4 8 181 53 226 6.91e-44 145
MS.gene045389.t1 AT1G55260 43.258 178 90 5 8 181 53 223 2.21e-43 144
MS.gene045389.t1 AT2G44300 39.796 196 96 5 7 183 11 203 1.38e-40 136
MS.gene045389.t1 AT2G44290 38.579 197 96 4 9 183 11 204 1.60e-40 136
MS.gene045389.t1 AT3G58550 38.926 149 84 4 4 145 9 157 6.51e-30 108
MS.gene045389.t1 AT1G03103 31.915 94 64 0 8 101 2 95 1.91e-14 68.2
MS.gene045389.t1 AT5G09370 37.736 106 61 2 24 127 21 123 3.16e-14 66.6
MS.gene045389.t1 AT5G09370 37.600 125 64 5 24 138 21 141 3.74e-14 67.0
MS.gene045389.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene045389.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene045389.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene045389.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene045389.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene045389.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene045389.t1 AT5G09370 39.175 97 54 2 24 118 21 114 6.05e-14 66.6
MS.gene045389.t1 AT1G73890 39.189 74 45 0 31 104 30 103 7.41e-14 67.0
MS.gene045389.t1 AT3G22600 28.188 149 95 5 10 150 6 150 2.51e-12 62.4
MS.gene045389.t1 AT4G14815 30.000 90 62 1 12 101 7 95 4.42e-11 58.9

Find 51 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATAATTACCGCTGGAAAT+AGG 0.158824 3.4:+78618045 None:intergenic
TCTTATCAAGGACTTGTTTA+AGG 0.173161 3.4:+78618737 None:intergenic
ACCTTAGCTTCAGGGGATTT+AGG 0.238114 3.4:+78618111 None:intergenic
AATAATTACCGCTGGAAATA+GGG 0.274317 3.4:+78618046 None:intergenic
AAGGAGGTGGCTGGTGGCAG+AGG 0.280788 3.4:-78617886 MS.gene045389:CDS
ATTCAACTTGATACCAAGTT+TGG 0.282616 3.4:+78618672 None:intergenic
GATAAACTTGTTACTCTTGC+TGG 0.316761 3.4:-78618817 MS.gene045389:CDS
CCTTCAAACACCTTAGCTTC+AGG 0.355634 3.4:+78618102 None:intergenic
TTCAACTTGATACCAAGTTT+GGG 0.358716 3.4:+78618673 None:intergenic
ACACATTCAGTTATATTAGA+TGG 0.359046 3.4:+78618610 None:intergenic
TGGATTGGGAAGGAGGTGGC+TGG 0.375917 3.4:-78617895 MS.gene045389:CDS
AGGGGAGGAACTGTTTGTTT+TGG 0.377595 3.4:+78618065 None:intergenic
GAAAAGAGTGGTGGTGGATT+GGG 0.380896 3.4:-78617909 MS.gene045389:CDS
TGCATTATTATTTCTACTGT+TGG 0.388007 3.4:-78618887 MS.gene045389:CDS
TTATTATTTCTACTGTTGGC+TGG 0.403176 3.4:-78618883 MS.gene045389:CDS
CTTATCAAGGACTTGTTTAA+GGG 0.407090 3.4:+78618738 None:intergenic
GATGGTATGTGACAAGCGTT+TGG 0.413443 3.4:+78618628 None:intergenic
GGAAAAGAGTGGTGGTGGAT+TGG 0.435272 3.4:-78617910 MS.gene045389:CDS
GCTAAGGTGTTTGAAGGACT+TGG 0.437073 3.4:-78618096 MS.gene045389:CDS
GAGGTGGCTGGTGGCAGAGG+TGG 0.447551 3.4:-78617883 MS.gene045389:CDS
AAGTGAGATATGAAAAGAAA+TGG 0.450601 3.4:+78617846 None:intergenic
GGAAGTACAACAATCTATAG+TGG 0.456166 3.4:+78618759 None:intergenic
CTTCAAACACCTTAGCTTCA+GGG 0.472455 3.4:+78618103 None:intergenic
ACCTAAATCCCCTGAAGCTA+AGG 0.474094 3.4:-78618112 MS.gene045389:CDS
CCTGAAGCTAAGGTGTTTGA+AGG 0.476875 3.4:-78618102 MS.gene045389:CDS
GATCGCAATGACCCCAAACT+TGG 0.492547 3.4:-78618685 MS.gene045389:CDS
GTGGTGGATTGGGAAGGAGG+TGG 0.495091 3.4:-78617899 MS.gene045389:CDS
GTGGTGGTGGATTGGGAAGG+AGG 0.496077 3.4:-78617902 MS.gene045389:CDS
TCTGAGGAAAAGAGTGGTGG+TGG 0.499326 3.4:-78617915 MS.gene045389:CDS
TGTTTAAGGTTCTGCTGAGA+GGG 0.503753 3.4:-78617952 MS.gene045389:intron
GAAGTACAACAATCTATAGT+GGG 0.507467 3.4:+78618760 None:intergenic
ATTGGGAAGGAGGTGGCTGG+TGG 0.530641 3.4:-78617892 MS.gene045389:CDS
TCAAACTTGAACTTCAAGCT+AGG 0.546383 3.4:+78617817 None:intergenic
TTCAAACACCTTAGCTTCAG+GGG 0.554009 3.4:+78618104 None:intergenic
ATAATTACCGCTGGAAATAG+GGG 0.565206 3.4:+78618047 None:intergenic
GGTTGTCTTCCATACGTTGG+TGG 0.578596 3.4:-78618796 MS.gene045389:CDS
GCTGGTTGTCTTCCATACGT+TGG 0.583066 3.4:-78618799 MS.gene045389:CDS
TTGGCACTACCACCAACGTA+TGG 0.586024 3.4:+78618787 None:intergenic
TGGAAGCTGTACAGCAAGAG+TGG 0.593369 3.4:+78618648 None:intergenic
TCAACTTGATACCAAGTTTG+GGG 0.608436 3.4:+78618674 None:intergenic
ATGTTTAAGGTTCTGCTGAG+AGG 0.614875 3.4:-78617953 MS.gene045389:intron
AGAGTGGTGGTGGATTGGGA+AGG 0.615185 3.4:-78617905 MS.gene045389:CDS
GGGATCAAGCTCAAGTTCTG+AGG 0.620498 3.4:-78617931 MS.gene045389:CDS
GGTCATTGCGATCTTTGATG+AGG 0.621163 3.4:+78618695 None:intergenic
GTTTAAGGTTCTGCTGAGAG+GGG 0.633464 3.4:-78617951 MS.gene045389:intron
AAGTACAACAATCTATAGTG+GGG 0.642782 3.4:+78618761 None:intergenic
AGTTCCTCCCCTATTTCCAG+CGG 0.660557 3.4:-78618054 MS.gene045389:intron
ATTACCGCTGGAAATAGGGG+AGG 0.669828 3.4:+78618050 None:intergenic
TCAAGTTCTGAGGAAAAGAG+TGG 0.677014 3.4:-78617921 MS.gene045389:CDS
AGTACAACAATCTATAGTGG+GGG 0.691483 3.4:+78618762 None:intergenic
AGTTCTGAGGAAAAGAGTGG+TGG 0.693229 3.4:-78617918 MS.gene045389:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATGAGAATTTTTTTTTGT+TGG - chr3.4:78618490-78618509 MS.gene045389:intron 15.0%
!! AAAATTATAATAATTACCGC+TGG + chr3.4:78618708-78618727 None:intergenic 20.0%
!! ATATAAATAAGGCTTAATCA+TGG - chr3.4:78618373-78618392 MS.gene045389:intron 20.0%
!!! TAGTTTGCTTTATGTATTAA+GGG - chr3.4:78618198-78618217 MS.gene045389:intron 20.0%
!!! TTAGTTTGCTTTATGTATTA+AGG - chr3.4:78618197-78618216 MS.gene045389:intron 20.0%
! AAGTGAGATATGAAAAGAAA+TGG + chr3.4:78618900-78618919 None:intergenic 25.0%
! ACACATTCAGTTATATTAGA+TGG + chr3.4:78618136-78618155 None:intergenic 25.0%
! AGAACCTTAAACATTATTAG+AGG + chr3.4:78618784-78618803 None:intergenic 25.0%
! ATAATCTTCAAAAGTAAGTG+AGG - chr3.4:78618404-78618423 MS.gene045389:intron 25.0%
! TCTAATATAACTGAATGTGT+AGG - chr3.4:78618136-78618155 MS.gene045389:intron 25.0%
!!! CACTTACTTTTGAAGATTAT+TGG + chr3.4:78618404-78618423 None:intergenic 25.0%
!!! GAGAATTTTTTTTTGTTGGT+CGG - chr3.4:78618494-78618513 MS.gene045389:intron 25.0%
!!! TGCATTATTATTTCTACTGT+TGG - chr3.4:78617856-78617875 MS.gene045389:CDS 25.0%
AAGTACAACAATCTATAGTG+GGG + chr3.4:78617985-78618004 None:intergenic 30.0%
AATAATTACCGCTGGAAATA+GGG + chr3.4:78618700-78618719 None:intergenic 30.0%
ATTCAACTTGATACCAAGTT+TGG + chr3.4:78618074-78618093 None:intergenic 30.0%
CATATGTATATAGTCCAATC+AGG + chr3.4:78618224-78618243 None:intergenic 30.0%
GAAGTACAACAATCTATAGT+GGG + chr3.4:78617986-78618005 None:intergenic 30.0%
TAATAATTACCGCTGGAAAT+AGG + chr3.4:78618701-78618720 None:intergenic 30.0%
TGTGCCTCTAATAATGTTTA+AGG - chr3.4:78618777-78618796 MS.gene045389:CDS 30.0%
TTCAACTTGATACCAAGTTT+GGG + chr3.4:78618073-78618092 None:intergenic 30.0%
! CTTATCAAGGACTTGTTTAA+GGG + chr3.4:78618008-78618027 None:intergenic 30.0%
! TAATGCAGTTACACTTTTGT+TGG + chr3.4:78617843-78617862 None:intergenic 30.0%
! TCTTATCAAGGACTTGTTTA+AGG + chr3.4:78618009-78618028 None:intergenic 30.0%
!! ACATTCCATTGAGTTGAATT+AGG + chr3.4:78618566-78618585 None:intergenic 30.0%
!! ACTTTTGAAGATTATTGGAC+CGG + chr3.4:78618399-78618418 None:intergenic 30.0%
!! TTATTATTTCTACTGTTGGC+TGG - chr3.4:78617860-78617879 MS.gene045389:CDS 30.0%
!!! TGCATTTTTTGGTCTTATCA+AGG + chr3.4:78618021-78618040 None:intergenic 30.0%
AAATAAGGCTTAATCATGGC+CGG - chr3.4:78618377-78618396 MS.gene045389:intron 35.0%
AATGTCTCATGTGACAAAAG+AGG + chr3.4:78618456-78618475 None:intergenic 35.0%
AGTACAACAATCTATAGTGG+GGG + chr3.4:78617984-78618003 None:intergenic 35.0%
ATAATTACCGCTGGAAATAG+GGG + chr3.4:78618699-78618718 None:intergenic 35.0%
TCAACTTGATACCAAGTTTG+GGG + chr3.4:78618072-78618091 None:intergenic 35.0%
TTATGTATTAAGGGCCTGAT+TGG - chr3.4:78618207-78618226 MS.gene045389:intron 35.0%
! GGAAGTACAACAATCTATAG+TGG + chr3.4:78617987-78618006 None:intergenic 35.0%
!! GATAAACTTGTTACTCTTGC+TGG - chr3.4:78617926-78617945 MS.gene045389:CDS 35.0%
!!! GAGGATACAGATGCATTTTT+TGG + chr3.4:78618032-78618051 None:intergenic 35.0%
CTTCAAACACCTTAGCTTCA+GGG + chr3.4:78618643-78618662 None:intergenic 40.0%
GGTGACAAATGTAGAAGATC+TGG + chr3.4:78618614-78618633 None:intergenic 40.0%
TTCAAACACCTTAGCTTCAG+GGG + chr3.4:78618642-78618661 None:intergenic 40.0%
! ACAATCTATAGTGGGGGTAT+TGG + chr3.4:78617978-78617997 None:intergenic 40.0%
! ATGTTTAAGGTTCTGCTGAG+AGG - chr3.4:78618790-78618809 MS.gene045389:CDS 40.0%
! TGTTTAAGGTTCTGCTGAGA+GGG - chr3.4:78618791-78618810 MS.gene045389:CDS 40.0%
!! ATTGAGTTGAATTAGGCACG+GGG + chr3.4:78618559-78618578 None:intergenic 40.0%
!! CATTGAGTTGAATTAGGCAC+GGG + chr3.4:78618560-78618579 None:intergenic 40.0%
!! CCATTGAGTTGAATTAGGCA+CGG + chr3.4:78618561-78618580 None:intergenic 40.0%
!! TAGTAGCTTTACTTTTGCGC+CGG - chr3.4:78618533-78618552 MS.gene045389:intron 40.0%
!! TCAAGTTCTGAGGAAAAGAG+TGG - chr3.4:78618822-78618841 MS.gene045389:CDS 40.0%
ACCTAAATCCCCTGAAGCTA+AGG - chr3.4:78618631-78618650 MS.gene045389:CDS 45.0%
ACCTTAGCTTCAGGGGATTT+AGG + chr3.4:78618635-78618654 None:intergenic 45.0%
CCGTGCCTAATTCAACTCAA+TGG - chr3.4:78618558-78618577 MS.gene045389:intron 45.0%
CCTTCAAACACCTTAGCTTC+AGG + chr3.4:78618644-78618663 None:intergenic 45.0%
GATGGTATGTGACAAGCGTT+TGG + chr3.4:78618118-78618137 None:intergenic 45.0%
GGTCATTGCGATCTTTGATG+AGG + chr3.4:78618051-78618070 None:intergenic 45.0%
! AGGGGAGGAACTGTTTGTTT+TGG + chr3.4:78618681-78618700 None:intergenic 45.0%
! CCTGAAGCTAAGGTGTTTGA+AGG - chr3.4:78618641-78618660 MS.gene045389:CDS 45.0%
! GCTAAGGTGTTTGAAGGACT+TGG - chr3.4:78618647-78618666 MS.gene045389:CDS 45.0%
! GTTTAAGGTTCTGCTGAGAG+GGG - chr3.4:78618792-78618811 MS.gene045389:CDS 45.0%
!! AGTTCTGAGGAAAAGAGTGG+TGG - chr3.4:78618825-78618844 MS.gene045389:CDS 45.0%
!! GAAAAGAGTGGTGGTGGATT+GGG - chr3.4:78618834-78618853 MS.gene045389:CDS 45.0%
!! GCTACTAAAGCCGTCGTTTT+TGG + chr3.4:78618520-78618539 None:intergenic 45.0%
!! TTAAAATGTTTATATAAATA+AGG - chr3.4:78618362-78618381 MS.gene045389:intron 5.0%
AGTTCCTCCCCTATTTCCAG+CGG - chr3.4:78618689-78618708 MS.gene045389:CDS 50.0%
ATTACCGCTGGAAATAGGGG+AGG + chr3.4:78618696-78618715 None:intergenic 50.0%
GATCGCAATGACCCCAAACT+TGG - chr3.4:78618058-78618077 MS.gene045389:CDS 50.0%
GCTGGTTGTCTTCCATACGT+TGG - chr3.4:78617944-78617963 MS.gene045389:CDS 50.0%
GGGATCAAGCTCAAGTTCTG+AGG - chr3.4:78618812-78618831 MS.gene045389:CDS 50.0%
GGTTGTCTTCCATACGTTGG+TGG - chr3.4:78617947-78617966 MS.gene045389:CDS 50.0%
TCTGAGGAAAAGAGTGGTGG+TGG - chr3.4:78618828-78618847 MS.gene045389:CDS 50.0%
TGGAAGCTGTACAGCAAGAG+TGG + chr3.4:78618098-78618117 None:intergenic 50.0%
TGTTGGTCGGCCAAAAACGA+CGG - chr3.4:78618507-78618526 MS.gene045389:intron 50.0%
TTGGCACTACCACCAACGTA+TGG + chr3.4:78617959-78617978 None:intergenic 50.0%
!! GGAAAAGAGTGGTGGTGGAT+TGG - chr3.4:78618833-78618852 MS.gene045389:CDS 50.0%
AGTTGAATTAGGCACGGGGC+CGG + chr3.4:78618555-78618574 None:intergenic 55.0%
!! AGAGTGGTGGTGGATTGGGA+AGG - chr3.4:78618838-78618857 MS.gene045389:CDS 55.0%
ATTGGGAAGGAGGTGGCTGG+TGG - chr3.4:78618851-78618870 MS.gene045389:CDS 60.0%
! TGGATTGGGAAGGAGGTGGC+TGG - chr3.4:78618848-78618867 MS.gene045389:CDS 60.0%
!! GTGGTGGATTGGGAAGGAGG+TGG - chr3.4:78618844-78618863 MS.gene045389:CDS 60.0%
!! GTGGTGGTGGATTGGGAAGG+AGG - chr3.4:78618841-78618860 MS.gene045389:CDS 60.0%
AAGGAGGTGGCTGGTGGCAG+AGG - chr3.4:78618857-78618876 MS.gene045389:CDS 65.0%
GAGGTGGCTGGTGGCAGAGG+TGG - chr3.4:78618860-78618879 MS.gene045389:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 78617830 78618935 78617830 ID=MS.gene045389
chr3.4 mRNA 78617830 78618935 78617830 ID=MS.gene045389.t1;Parent=MS.gene045389
chr3.4 exon 78618614 78618935 78618614 ID=MS.gene045389.t1.exon1;Parent=MS.gene045389.t1
chr3.4 CDS 78618614 78618935 78618614 ID=cds.MS.gene045389.t1;Parent=MS.gene045389.t1
chr3.4 exon 78618055 78618150 78618055 ID=MS.gene045389.t1.exon2;Parent=MS.gene045389.t1
chr3.4 CDS 78618055 78618150 78618055 ID=cds.MS.gene045389.t1;Parent=MS.gene045389.t1
chr3.4 exon 78617830 78617966 78617830 ID=MS.gene045389.t1.exon3;Parent=MS.gene045389.t1
chr3.4 CDS 78617830 78617966 78617830 ID=cds.MS.gene045389.t1;Parent=MS.gene045389.t1
Gene Sequence

>MS.gene045389

ATGATGGTTTCCAACAAAAGTGTAACTGCATTATTATTTCTACTGTTGGCTGGTTTTGTGAGCTCAGATTTTACAAAAGACAGAAACGATTGTGCTGATAAACTTGTTACTCTTGCTGGTTGTCTTCCATACGTTGGTGGTAGTGCCAATACCCCCACTATAGATTGTTGTACTTCCCTTAAACAAGTCCTTGATAAGACCAAAAAATGCATCTGTATCCTCATCAAAGATCGCAATGACCCCAAACTTGGTATCAAGTTGAATGCCACTCTTGCTGTACAGCTTCCAAACGCTTGTCACATACCATCTAATATAACTGAATGTGTAGGCAAGTATATTTTTCTCATGATCTTATCTAGCTATTAGTTTAGTTTGCTTTATGTATTAAGGGCCTGATTGGACTATATACATATGCTGTTTAAGCTGATTTTCATAAACTTTTCAAATAGCTTATGAAAACAACTTATAGATTATACAGAAATAATTTGACTTTATTATACATAAATGATATACGCTATAATATAAGTGTTTATTAAAATGTTTATATAAATAAGGCTTAATCATGGCCGGTCCAATAATCTTCAAAAGTAAGTGAGGCATAAAGCGAAGTTCAATATATGTCTCCTCTTTTGTCACATGAGACATTTTTCTTGTAAAATCTAATGAGAATTTTTTTTTGTTGGTCGGCCAAAAACGACGGCTTTAGTAGCTTTACTTTTGCGCCGGCCCCGTGCCTAATTCAACTCAATGGAATGTTTAATTTATACTTTTGTATTTTTTTCCAGATCTTCTACATTTGTCACCTAAATCCCCTGAAGCTAAGGTGTTTGAAGGACTTGGAAATTCTACCAAAACAAACAGTTCCTCCCCTATTTCCAGCGGTAATTATTATAATTTTAGTATTGTTATTGAGTAATTTTTGCGTGTATTGAAAATTGAAGCTAATTTGTGCCTCTAATAATGTTTAAGGTTCTGCTGAGAGGGGATCAAGCTCAAGTTCTGAGGAAAAGAGTGGTGGTGGATTGGGAAGGAGGTGGCTGGTGGCAGAGGTGGTTTGTGCTATTTTACCATTTCTTTTCATATCTCACTTCTTCATCCTAGCTTGA

Protein sequence

>MS.gene045389.t1

MMVSNKSVTALLFLLLAGFVSSDFTKDRNDCADKLVTLAGCLPYVGGSANTPTIDCCTSLKQVLDKTKKCICILIKDRNDPKLGIKLNATLAVQLPNACHIPSNITEYLLHLSPKSPEAKVFEGLGNSTKTNSSSPISSGSAERGSSSSSEEKSGGGLGRRWLVAEVVCAILPFLFISHFFILA