Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066915.t1 | XP_013445286.1 | 95.7 | 187 | 8 | 0 | 1 | 187 | 9 | 195 | 1.80E-95 | 358.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066915.t1 | O64864 | 44.4 | 189 | 89 | 3 | 5 | 178 | 15 | 202 | 1.2e-39 | 164.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066915.t1 | A0A072TPI6 | 95.7 | 187 | 8 | 0 | 1 | 187 | 9 | 195 | 1.3e-95 | 358.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene08939 | MS.gene066915 | PPI |
| MS.gene79864 | MS.gene066915 | PPI |
| MS.gene023520 | MS.gene066915 | PPI |
| MS.gene77503 | MS.gene066915 | PPI |
| MS.gene75704 | MS.gene066915 | PPI |
| MS.gene066915 | MS.gene77500 | PPI |
| MS.gene066915 | MS.gene09303 | PPI |
| MS.gene066915 | MS.gene045389 | PPI |
| MS.gene08785 | MS.gene066915 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066915.t1 | MTR_8g446830 | 95.722 | 187 | 8 | 0 | 1 | 187 | 9 | 195 | 1.62e-132 | 369 |
| MS.gene066915.t1 | MTR_7g014880 | 47.849 | 186 | 84 | 5 | 1 | 177 | 10 | 191 | 8.59e-51 | 162 |
| MS.gene066915.t1 | MTR_8g085560 | 40.556 | 180 | 99 | 4 | 5 | 178 | 15 | 192 | 6.50e-40 | 134 |
| MS.gene066915.t1 | MTR_3g083760 | 51.304 | 115 | 55 | 1 | 13 | 127 | 18 | 131 | 6.50e-36 | 122 |
| MS.gene066915.t1 | MTR_3g083760 | 44.697 | 132 | 55 | 2 | 13 | 127 | 18 | 148 | 1.78e-31 | 113 |
| MS.gene066915.t1 | MTR_3g085210 | 32.231 | 121 | 78 | 3 | 4 | 120 | 9 | 129 | 4.77e-13 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066915.t1 | AT2G44300 | 50.568 | 176 | 71 | 3 | 18 | 178 | 27 | 201 | 9.96e-58 | 180 |
| MS.gene066915.t1 | AT2G44290 | 45.503 | 189 | 87 | 3 | 5 | 178 | 15 | 202 | 1.27e-56 | 177 |
| MS.gene066915.t1 | AT1G55260 | 35.429 | 175 | 109 | 4 | 5 | 178 | 55 | 226 | 1.43e-34 | 122 |
| MS.gene066915.t1 | AT1G55260 | 36.571 | 175 | 104 | 5 | 5 | 178 | 55 | 223 | 2.47e-34 | 121 |
| MS.gene066915.t1 | AT3G58550 | 39.007 | 141 | 82 | 3 | 1 | 139 | 14 | 152 | 5.58e-28 | 103 |
| MS.gene066915.t1 | AT1G73890 | 40.506 | 79 | 46 | 1 | 26 | 104 | 30 | 107 | 9.61e-13 | 63.9 |
| MS.gene066915.t1 | AT1G03103 | 28.324 | 173 | 111 | 4 | 6 | 174 | 5 | 168 | 5.43e-12 | 61.6 |
Find 35 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTTGAGCCTCAGCTGATT+TGG | 0.222383 | 8.4:+54868857 | None:intergenic |
| TGTGCATTCTTGTTTATCTT+TGG | 0.235145 | 8.4:+54869353 | None:intergenic |
| ACCAGGACATTTAGAGATAT+TGG | 0.273723 | 8.4:+54869116 | None:intergenic |
| GAATGCACAGCTCAATTAAC+AGG | 0.295795 | 8.4:-54869339 | MS.gene066915:CDS |
| AGATCGTAATGATCCTGATT+TGG | 0.316207 | 8.4:-54869199 | MS.gene066915:CDS |
| AGAAAAGAGAAGAGATTGAT+TGG | 0.339081 | 8.4:-54868303 | MS.gene066915:CDS |
| ACTTGAGCCTCAGCTGATTT+GGG | 0.345043 | 8.4:+54868858 | None:intergenic |
| CTACTAGCATCAATGATAGT+TGG | 0.383793 | 8.4:-54869396 | MS.gene066915:CDS |
| GATCGTAATGATCCTGATTT+GGG | 0.411730 | 8.4:-54869198 | MS.gene066915:CDS |
| TACTAGCATCAATGATAGTT+GGG | 0.439367 | 8.4:-54869395 | MS.gene066915:CDS |
| TATAGTTATTAATGTTACTT+TGG | 0.452178 | 8.4:-54869172 | MS.gene066915:CDS |
| TCTGTGTAGTACTTCTTGCT+TGG | 0.453220 | 8.4:+54868340 | None:intergenic |
| TCATGCCTCCCCTATGTTGA+AGG | 0.464755 | 8.4:-54869309 | MS.gene066915:CDS |
| GCCAATATCTCTAAATGTCC+TGG | 0.474666 | 8.4:-54869117 | MS.gene066915:intron |
| TTTCCTTCACCTTCAACATA+GGG | 0.478002 | 8.4:+54869300 | None:intergenic |
| GGACATTTAGAGATATTGGC+AGG | 0.478911 | 8.4:+54869120 | None:intergenic |
| ATAACTATACCACCCAAATC+AGG | 0.482338 | 8.4:+54869186 | None:intergenic |
| AGTGCTGCACCTAGTCCATC+AGG | 0.483330 | 8.4:-54868795 | MS.gene066915:intron |
| AGCACTTGGACTAATATTGC+TGG | 0.486730 | 8.4:+54868812 | None:intergenic |
| TGAATTAGAGCTGCTGCACA+TGG | 0.506732 | 8.4:-54868883 | MS.gene066915:intron |
| TTGAGACCATCACAACAATC+TGG | 0.516643 | 8.4:+54869267 | None:intergenic |
| GCAGGGGCATTACAAACTGT+TGG | 0.517485 | 8.4:+54869138 | None:intergenic |
| GCATCAATGATAGTTGGGAT+TGG | 0.519091 | 8.4:-54869390 | MS.gene066915:CDS |
| CCATCACAACAATCTGGTGC+TGG | 0.553657 | 8.4:+54869273 | None:intergenic |
| AGTTTCATACCTGATGGACT+AGG | 0.561670 | 8.4:+54868786 | None:intergenic |
| CTCCCCTATGTTGAAGGTGA+AGG | 0.561730 | 8.4:-54869303 | MS.gene066915:CDS |
| CCAGCACCAGATTGTTGTGA+TGG | 0.573645 | 8.4:-54869273 | MS.gene066915:CDS |
| ACATTTAGAGATATTGGCAG+GGG | 0.578847 | 8.4:+54869122 | None:intergenic |
| GATGGACTAGGTGCAGCACT+TGG | 0.582516 | 8.4:+54868798 | None:intergenic |
| GACATTTAGAGATATTGGCA+GGG | 0.601482 | 8.4:+54869121 | None:intergenic |
| TTCCTTCACCTTCAACATAG+GGG | 0.602068 | 8.4:+54869301 | None:intergenic |
| CTTCACCTTCAACATAGGGG+AGG | 0.615427 | 8.4:+54869304 | None:intergenic |
| CGTAATGATCCTGATTTGGG+TGG | 0.647273 | 8.4:-54869195 | MS.gene066915:CDS |
| CATGGATCCCAAATCAGCTG+AGG | 0.670451 | 8.4:-54868865 | MS.gene066915:CDS |
| AATGCACAGCTCAATTAACA+GGG | 0.714051 | 8.4:-54869338 | MS.gene066915:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATTCAATAATTTTTAG+AGG | - | chr8.4:54869148-54869167 | MS.gene066915:CDS | 10.0% |
| !!! | GATATTTTTTTTTTTTTTGT+TGG | - | chr8.4:54868613-54868632 | MS.gene066915:intron | 10.0% |
| !! | AATGACAAATAATAAAATTG+GGG | - | chr8.4:54869202-54869221 | MS.gene066915:CDS | 15.0% |
| !! | CAATGACAAATAATAAAATT+GGG | - | chr8.4:54869201-54869220 | MS.gene066915:CDS | 15.0% |
| !! | TAAATTATTGCATATTTGTT+TGG | + | chr8.4:54869093-54869112 | None:intergenic | 15.0% |
| !! | TATGTATATATAAATTGTAG+AGG | + | chr8.4:54868958-54868977 | None:intergenic | 15.0% |
| !! | TCAATGACAAATAATAAAAT+TGG | - | chr8.4:54869200-54869219 | MS.gene066915:CDS | 15.0% |
| !!! | GTTTTTTATCAATTAAACAA+TGG | - | chr8.4:54868804-54868823 | MS.gene066915:CDS | 15.0% |
| !!! | TATAGTTATTAATGTTACTT+TGG | - | chr8.4:54868469-54868488 | MS.gene066915:intron | 15.0% |
| !!! | TTATTTCTCAATAATTTTAG+AGG | - | chr8.4:54869110-54869129 | MS.gene066915:intron | 15.0% |
| !! | AAATGAGTCAAATTGATTAT+GGG | - | chr8.4:54868591-54868610 | MS.gene066915:intron | 20.0% |
| !! | ATGACAAATAATAAAATTGG+GGG | - | chr8.4:54869203-54869222 | MS.gene066915:CDS | 20.0% |
| !! | ATTAGCAAAAAATCACAATT+AGG | + | chr8.4:54869238-54869257 | None:intergenic | 20.0% |
| !! | GGATATAACATAACATTAAA+AGG | - | chr8.4:54868717-54868736 | MS.gene066915:intron | 20.0% |
| !! | TTAGCAAAAAATCACAATTA+GGG | + | chr8.4:54869237-54869256 | None:intergenic | 20.0% |
| !!! | GTTTTAGTTTGGTTTTTATT+TGG | - | chr8.4:54869383-54869402 | MS.gene066915:CDS | 20.0% |
| !!! | TTTTTTTTTTTGTTGGTAGT+CGG | - | chr8.4:54868620-54868639 | MS.gene066915:intron | 20.0% |
| ! | GAAATGAGTCAAATTGATTA+TGG | - | chr8.4:54868590-54868609 | MS.gene066915:intron | 25.0% |
| ! | TAACATAGAAAAACTTTACC+AGG | + | chr8.4:54868545-54868564 | None:intergenic | 25.0% |
| ! | TGACAAATAATAAAATTGGG+GGG | - | chr8.4:54869204-54869223 | MS.gene066915:CDS | 25.0% |
| !!! | TTTTTTTTTTGTTGGTAGTC+GGG | - | chr8.4:54868621-54868640 | MS.gene066915:intron | 25.0% |
| AGAAAAGAGAAGAGATTGAT+TGG | - | chr8.4:54869338-54869357 | MS.gene066915:CDS | 30.0% | |
| TACTAGCATCAATGATAGTT+GGG | - | chr8.4:54868246-54868265 | MS.gene066915:CDS | 30.0% | |
| ! | GTTTAGACGTTTTGTCTATT+GGG | - | chr8.4:54869360-54869379 | MS.gene066915:CDS | 30.0% |
| ! | TGTGCATTCTTGTTTATCTT+TGG | + | chr8.4:54868291-54868310 | None:intergenic | 30.0% |
| ! | TTTAGACGTTTTGTCTATTG+GGG | - | chr8.4:54869361-54869380 | MS.gene066915:CDS | 30.0% |
| !!! | TTTTTTTTTGTTGGTAGTCG+GGG | - | chr8.4:54868622-54868641 | MS.gene066915:intron | 30.0% |
| AATGCACAGCTCAATTAACA+GGG | - | chr8.4:54868303-54868322 | MS.gene066915:CDS | 35.0% | |
| ACATTTAGAGATATTGGCAG+GGG | + | chr8.4:54868522-54868541 | None:intergenic | 35.0% | |
| ACCAGGACATTTAGAGATAT+TGG | + | chr8.4:54868528-54868547 | None:intergenic | 35.0% | |
| AGATCGTAATGATCCTGATT+TGG | - | chr8.4:54868442-54868461 | MS.gene066915:intron | 35.0% | |
| ATAACTATACCACCCAAATC+AGG | + | chr8.4:54868458-54868477 | None:intergenic | 35.0% | |
| CTACTAGCATCAATGATAGT+TGG | - | chr8.4:54868245-54868264 | MS.gene066915:CDS | 35.0% | |
| GACATTTAGAGATATTGGCA+GGG | + | chr8.4:54868523-54868542 | None:intergenic | 35.0% | |
| GATCGTAATGATCCTGATTT+GGG | - | chr8.4:54868443-54868462 | MS.gene066915:intron | 35.0% | |
| TTTCCTTCACCTTCAACATA+GGG | + | chr8.4:54868344-54868363 | None:intergenic | 35.0% | |
| ! | AGAGTGTCATTCATGTTATG+TGG | - | chr8.4:54869259-54869278 | MS.gene066915:CDS | 35.0% |
| ! | GGTTTAGACGTTTTGTCTAT+TGG | - | chr8.4:54869359-54869378 | MS.gene066915:CDS | 35.0% |
| ! | TTTTCCTTCACCTTCAACAT+AGG | + | chr8.4:54868345-54868364 | None:intergenic | 35.0% |
| !!! | TGTCTATTGGGGTTTTAGTT+TGG | - | chr8.4:54869372-54869391 | MS.gene066915:CDS | 35.0% |
| AAGCGAAGTTTCATACCTGA+TGG | + | chr8.4:54868864-54868883 | None:intergenic | 40.0% | |
| AAGCTCAAGAGAACTGATCA+TGG | - | chr8.4:54868695-54868714 | MS.gene066915:intron | 40.0% | |
| AGCACTTGGACTAATATTGC+TGG | + | chr8.4:54868832-54868851 | None:intergenic | 40.0% | |
| AGCTCAAGAGAACTGATCAT+GGG | - | chr8.4:54868696-54868715 | MS.gene066915:intron | 40.0% | |
| GAATGCACAGCTCAATTAAC+AGG | - | chr8.4:54868302-54868321 | MS.gene066915:CDS | 40.0% | |
| GCATCAATGATAGTTGGGAT+TGG | - | chr8.4:54868251-54868270 | MS.gene066915:CDS | 40.0% | |
| GCCAATATCTCTAAATGTCC+TGG | - | chr8.4:54868524-54868543 | MS.gene066915:intron | 40.0% | |
| GGACATTTAGAGATATTGGC+AGG | + | chr8.4:54868524-54868543 | None:intergenic | 40.0% | |
| TCATGTTATGTGGCTGTAGA+TGG | - | chr8.4:54869269-54869288 | MS.gene066915:CDS | 40.0% | |
| TCTCTTGAGCTTAACTCAGT+TGG | + | chr8.4:54868687-54868706 | None:intergenic | 40.0% | |
| TCTGTGTAGTACTTCTTGCT+TGG | + | chr8.4:54869304-54869323 | None:intergenic | 40.0% | |
| TTCCTTCACCTTCAACATAG+GGG | + | chr8.4:54868343-54868362 | None:intergenic | 40.0% | |
| TTGAGACCATCACAACAATC+TGG | + | chr8.4:54868377-54868396 | None:intergenic | 40.0% | |
| !! | AGTTTCATACCTGATGGACT+AGG | + | chr8.4:54868858-54868877 | None:intergenic | 40.0% |
| !!! | TTTTATTTGGCTCTGCTGCT+GGG | - | chr8.4:54869396-54869415 | MS.gene066915:CDS | 40.0% |
| !!! | TTTTTATTTGGCTCTGCTGC+TGG | - | chr8.4:54869395-54869414 | MS.gene066915:CDS | 40.0% |
| AACTTGAGCCTCAGCTGATT+TGG | + | chr8.4:54868787-54868806 | None:intergenic | 45.0% | |
| TGAATTAGAGCTGCTGCACA+TGG | - | chr8.4:54868758-54868777 | MS.gene066915:intron | 45.0% | |
| ! | ACTTGAGCCTCAGCTGATTT+GGG | + | chr8.4:54868786-54868805 | None:intergenic | 45.0% |
| !! | CGTAATGATCCTGATTTGGG+TGG | - | chr8.4:54868446-54868465 | MS.gene066915:intron | 45.0% |
| !!! | ATATAAAACAATTTTATAAT+AGG | + | chr8.4:54868996-54869015 | None:intergenic | 5.0% |
| CATGGATCCCAAATCAGCTG+AGG | - | chr8.4:54868776-54868795 | MS.gene066915:intron | 50.0% | |
| CCAGCACCAGATTGTTGTGA+TGG | - | chr8.4:54868368-54868387 | MS.gene066915:intron | 50.0% | |
| CCATCACAACAATCTGGTGC+TGG | + | chr8.4:54868371-54868390 | None:intergenic | 50.0% | |
| CTCCCCTATGTTGAAGGTGA+AGG | - | chr8.4:54868338-54868357 | MS.gene066915:CDS | 50.0% | |
| CTTCACCTTCAACATAGGGG+AGG | + | chr8.4:54868340-54868359 | None:intergenic | 50.0% | |
| TCATGCCTCCCCTATGTTGA+AGG | - | chr8.4:54868332-54868351 | MS.gene066915:CDS | 50.0% | |
| TGGTAGTCGGGGTTTGAACT+CGG | - | chr8.4:54868633-54868652 | MS.gene066915:intron | 50.0% | |
| !! | GCAGGGGCATTACAAACTGT+TGG | + | chr8.4:54868506-54868525 | None:intergenic | 50.0% |
| AGTGCTGCACCTAGTCCATC+AGG | - | chr8.4:54868846-54868865 | MS.gene066915:CDS | 55.0% | |
| GGTAGTCGGGGTTTGAACTC+GGG | - | chr8.4:54868634-54868653 | MS.gene066915:intron | 55.0% | |
| !! | GATGGACTAGGTGCAGCACT+TGG | + | chr8.4:54868846-54868865 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 54868230 | 54869433 | 54868230 | ID=MS.gene066915 |
| chr8.4 | mRNA | 54868230 | 54869433 | 54868230 | ID=MS.gene066915.t1;Parent=MS.gene066915 |
| chr8.4 | exon | 54869118 | 54869433 | 54869118 | ID=MS.gene066915.t1.exon1;Parent=MS.gene066915.t1 |
| chr8.4 | CDS | 54869118 | 54869433 | 54869118 | ID=cds.MS.gene066915.t1;Parent=MS.gene066915.t1 |
| chr8.4 | exon | 54868796 | 54868897 | 54868796 | ID=MS.gene066915.t1.exon2;Parent=MS.gene066915.t1 |
| chr8.4 | CDS | 54868796 | 54868897 | 54868796 | ID=cds.MS.gene066915.t1;Parent=MS.gene066915.t1 |
| chr8.4 | exon | 54868230 | 54868375 | 54868230 | ID=MS.gene066915.t1.exon3;Parent=MS.gene066915.t1 |
| chr8.4 | CDS | 54868230 | 54868375 | 54868230 | ID=cds.MS.gene066915.t1;Parent=MS.gene066915.t1 |
| Gene Sequence |
| Protein sequence |
