Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067247.t1 | XP_013445286.1 | 95.4 | 195 | 9 | 0 | 1 | 195 | 1 | 195 | 5.50E-100 | 373.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067247.t1 | O64864 | 44.7 | 197 | 91 | 4 | 7 | 186 | 7 | 202 | 9.3e-40 | 164.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067247.t1 | A0A072TPI6 | 95.4 | 195 | 9 | 0 | 1 | 195 | 1 | 195 | 4.0e-100 | 373.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene067247 | MS.gene08785 | PPI |
| MS.gene023520 | MS.gene067247 | PPI |
| MS.gene067247 | MS.gene045389 | PPI |
| MS.gene067247 | MS.gene77500 | PPI |
| MS.gene067247 | MS.gene09303 | PPI |
| MS.gene75704 | MS.gene067247 | PPI |
| MS.gene77503 | MS.gene067247 | PPI |
| MS.gene08939 | MS.gene067247 | PPI |
| MS.gene79864 | MS.gene067247 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067247.t1 | MTR_8g446830 | 95.385 | 195 | 9 | 0 | 1 | 195 | 1 | 195 | 6.80e-139 | 385 |
| MS.gene067247.t1 | MTR_7g014880 | 47.849 | 186 | 84 | 5 | 9 | 185 | 10 | 191 | 1.00e-50 | 162 |
| MS.gene067247.t1 | MTR_8g085560 | 40.556 | 180 | 99 | 4 | 13 | 186 | 15 | 192 | 9.48e-40 | 134 |
| MS.gene067247.t1 | MTR_3g083760 | 46.667 | 135 | 68 | 2 | 1 | 135 | 1 | 131 | 7.33e-36 | 123 |
| MS.gene067247.t1 | MTR_3g083760 | 44.697 | 132 | 55 | 2 | 21 | 135 | 18 | 148 | 1.84e-31 | 113 |
| MS.gene067247.t1 | MTR_3g085210 | 32.231 | 121 | 78 | 3 | 12 | 128 | 9 | 129 | 3.80e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067247.t1 | AT2G44300 | 50.568 | 176 | 71 | 3 | 26 | 186 | 27 | 201 | 1.35e-57 | 180 |
| MS.gene067247.t1 | AT2G44290 | 44.828 | 203 | 94 | 4 | 1 | 186 | 1 | 202 | 8.67e-57 | 178 |
| MS.gene067247.t1 | AT1G55260 | 34.426 | 183 | 116 | 4 | 5 | 186 | 47 | 226 | 6.39e-35 | 123 |
| MS.gene067247.t1 | AT1G55260 | 35.519 | 183 | 111 | 5 | 5 | 186 | 47 | 223 | 1.19e-34 | 122 |
| MS.gene067247.t1 | AT3G58550 | 38.356 | 146 | 86 | 3 | 4 | 147 | 9 | 152 | 4.35e-29 | 106 |
| MS.gene067247.t1 | AT1G73890 | 40.506 | 79 | 46 | 1 | 34 | 112 | 30 | 107 | 1.02e-12 | 64.3 |
| MS.gene067247.t1 | AT1G03103 | 28.324 | 173 | 111 | 4 | 14 | 182 | 5 | 168 | 7.17e-12 | 61.6 |
| MS.gene067247.t1 | AT1G27950 | 27.097 | 155 | 102 | 4 | 4 | 152 | 5 | 154 | 6.12e-11 | 59.3 |
Find 35 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTTGAGCCTCAGCTGATT+TGG | 0.222383 | 8.1:+59158637 | None:intergenic |
| TGTGCATTCTTGTTTGTCTT+TGG | 0.244832 | 8.1:+59159131 | None:intergenic |
| ACCAGGACATTTAGAGATAT+TGG | 0.273723 | 8.1:+59158894 | None:intergenic |
| GAATGCACAGCTCAATTAAC+AGG | 0.295795 | 8.1:-59159117 | MS.gene067247:CDS |
| AGATCGTAATGATCCTGATT+TGG | 0.316207 | 8.1:-59158977 | MS.gene067247:CDS |
| AGAAAAGAGAAGAGATTGAT+TGG | 0.339081 | 8.1:-59158084 | MS.gene067247:CDS |
| ACTTGAGCCTCAGCTGATTT+GGG | 0.345043 | 8.1:+59158638 | None:intergenic |
| CTACTAGCATCAATGATAGT+TGG | 0.393050 | 8.1:-59159174 | MS.gene067247:CDS |
| GATCGTAATGATCCTGATTT+GGG | 0.411730 | 8.1:-59158976 | MS.gene067247:CDS |
| TACTAGCATCAATGATAGTT+GGG | 0.430888 | 8.1:-59159173 | MS.gene067247:CDS |
| TATAGTTATTAATGTTACTT+TGG | 0.452178 | 8.1:-59158950 | MS.gene067247:CDS |
| TCTGTGTAGTACTTCTTGCT+TGG | 0.453220 | 8.1:+59158121 | None:intergenic |
| TCATGTCTCCCCTATGTTGA+AGG | 0.464755 | 8.1:-59159087 | MS.gene067247:CDS |
| GCCAATATCTCTAAATGTCC+TGG | 0.474666 | 8.1:-59158895 | MS.gene067247:intron |
| TTTCCTTCACCTTCAACATA+GGG | 0.478002 | 8.1:+59159078 | None:intergenic |
| GGACATTTAGAGATATTGGC+AGG | 0.478911 | 8.1:+59158898 | None:intergenic |
| ATAACTATACCACCCAAATC+AGG | 0.482338 | 8.1:+59158964 | None:intergenic |
| AGTGCTGCACCTAGTCCATC+AGG | 0.483330 | 8.1:-59158575 | MS.gene067247:intron |
| AGCACTTGGACTAATATTGC+TGG | 0.486730 | 8.1:+59158592 | None:intergenic |
| TGAATTAGAGCTGCTGCACA+TGG | 0.506732 | 8.1:-59158663 | MS.gene067247:intron |
| TTGAGACCATCACAACAATC+TGG | 0.516643 | 8.1:+59159045 | None:intergenic |
| GCAGGGGCATTACAAACTGT+TGG | 0.517485 | 8.1:+59158916 | None:intergenic |
| GCATCAATGATAGTTGGGAT+TGG | 0.519091 | 8.1:-59159168 | MS.gene067247:CDS |
| CCATCACAACAATCTGGTGC+TGG | 0.553657 | 8.1:+59159051 | None:intergenic |
| AGTTTCATACCTGATGGACT+AGG | 0.561670 | 8.1:+59158566 | None:intergenic |
| CTCCCCTATGTTGAAGGTGA+AGG | 0.561730 | 8.1:-59159081 | MS.gene067247:CDS |
| CCAGCACCAGATTGTTGTGA+TGG | 0.573645 | 8.1:-59159051 | MS.gene067247:CDS |
| ACATTTAGAGATATTGGCAG+GGG | 0.578847 | 8.1:+59158900 | None:intergenic |
| GATGGACTAGGTGCAGCACT+TGG | 0.582516 | 8.1:+59158578 | None:intergenic |
| GACATTTAGAGATATTGGCA+GGG | 0.601482 | 8.1:+59158899 | None:intergenic |
| TTCCTTCACCTTCAACATAG+GGG | 0.609504 | 8.1:+59159079 | None:intergenic |
| CTACCACAACACAAAAGTCA+TGG | 0.619174 | 8.1:-59159208 | MS.gene067247:CDS |
| CGTAATGATCCTGATTTGGG+TGG | 0.647273 | 8.1:-59158973 | MS.gene067247:CDS |
| CATGGATCCCAAATCAGCTG+AGG | 0.670451 | 8.1:-59158645 | MS.gene067247:CDS |
| AATGCACAGCTCAATTAACA+GGG | 0.714051 | 8.1:-59159116 | MS.gene067247:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATTCAATAATTTTTAG+AGG | - | chr8.1:59158950-59158969 | MS.gene067247:CDS | 10.0% |
| !! | AATGACAAATAATAAAATTG+GGG | - | chr8.1:59159004-59159023 | MS.gene067247:CDS | 15.0% |
| !! | CAATGACAAATAATAAAATT+GGG | - | chr8.1:59159003-59159022 | MS.gene067247:CDS | 15.0% |
| !! | TAAATTATTGCATATTTGTT+TGG | + | chr8.1:59158895-59158914 | None:intergenic | 15.0% |
| !! | TATGTATATATAAATTGTAG+AGG | + | chr8.1:59158761-59158780 | None:intergenic | 15.0% |
| !! | TCAATGACAAATAATAAAAT+TGG | - | chr8.1:59159002-59159021 | MS.gene067247:CDS | 15.0% |
| !!! | GTTTTTTATCAATTAAACAA+TGG | - | chr8.1:59158607-59158626 | MS.gene067247:CDS | 15.0% |
| !!! | TATAGTTATTAATGTTACTT+TGG | - | chr8.1:59158274-59158293 | MS.gene067247:intron | 15.0% |
| !!! | TTATTTCTCAATAATTTTAG+AGG | - | chr8.1:59158912-59158931 | MS.gene067247:CDS | 15.0% |
| !! | AAATGAGTCAAATTGATTAT+GGG | - | chr8.1:59158396-59158415 | MS.gene067247:intron | 20.0% |
| !! | ATGACAAATAATAAAATTGG+GGG | - | chr8.1:59159005-59159024 | MS.gene067247:CDS | 20.0% |
| !! | ATTAGCAAAAAATCACAATT+AGG | + | chr8.1:59159040-59159059 | None:intergenic | 20.0% |
| !! | GGATATAACATAACATTAAA+AGG | - | chr8.1:59158520-59158539 | MS.gene067247:intron | 20.0% |
| !! | TTAGCAAAAAATCACAATTA+GGG | + | chr8.1:59159039-59159058 | None:intergenic | 20.0% |
| !!! | GGGATTTTTTTTTTTTTTGT+TGG | - | chr8.1:59158416-59158435 | MS.gene067247:intron | 20.0% |
| !!! | GTTTTAGTTTGGTTTTTATT+TGG | - | chr8.1:59159185-59159204 | MS.gene067247:CDS | 20.0% |
| !!! | TTTTTTTTTTTGTTGGTAGT+CGG | - | chr8.1:59158423-59158442 | MS.gene067247:intron | 20.0% |
| ! | GAAATGAGTCAAATTGATTA+TGG | - | chr8.1:59158395-59158414 | MS.gene067247:intron | 25.0% |
| ! | TAACATAGAAAAACTTTACC+AGG | + | chr8.1:59158350-59158369 | None:intergenic | 25.0% |
| ! | TGACAAATAATAAAATTGGG+GGG | - | chr8.1:59159006-59159025 | MS.gene067247:CDS | 25.0% |
| !!! | TTTTTTTTTTGTTGGTAGTC+GGG | - | chr8.1:59158424-59158443 | MS.gene067247:intron | 25.0% |
| AGAAAAGAGAAGAGATTGAT+TGG | - | chr8.1:59159140-59159159 | MS.gene067247:CDS | 30.0% | |
| TACTAGCATCAATGATAGTT+GGG | - | chr8.1:59158051-59158070 | MS.gene067247:CDS | 30.0% | |
| ! | GTTTAGACGTTTTGTCTATT+GGG | - | chr8.1:59159162-59159181 | MS.gene067247:CDS | 30.0% |
| ! | TTTAGACGTTTTGTCTATTG+GGG | - | chr8.1:59159163-59159182 | MS.gene067247:CDS | 30.0% |
| !!! | TTTTTTTTTGTTGGTAGTCG+GGG | - | chr8.1:59158425-59158444 | MS.gene067247:intron | 30.0% |
| AATGCACAGCTCAATTAACA+GGG | - | chr8.1:59158108-59158127 | MS.gene067247:CDS | 35.0% | |
| ACATTTAGAGATATTGGCAG+GGG | + | chr8.1:59158327-59158346 | None:intergenic | 35.0% | |
| ACCAGGACATTTAGAGATAT+TGG | + | chr8.1:59158333-59158352 | None:intergenic | 35.0% | |
| AGATCGTAATGATCCTGATT+TGG | - | chr8.1:59158247-59158266 | MS.gene067247:intron | 35.0% | |
| ATAACTATACCACCCAAATC+AGG | + | chr8.1:59158263-59158282 | None:intergenic | 35.0% | |
| CTACTAGCATCAATGATAGT+TGG | - | chr8.1:59158050-59158069 | MS.gene067247:CDS | 35.0% | |
| GACATTTAGAGATATTGGCA+GGG | + | chr8.1:59158328-59158347 | None:intergenic | 35.0% | |
| GATCGTAATGATCCTGATTT+GGG | - | chr8.1:59158248-59158267 | MS.gene067247:intron | 35.0% | |
| TTTCCTTCACCTTCAACATA+GGG | + | chr8.1:59158149-59158168 | None:intergenic | 35.0% | |
| ! | AGAGTGTCATTCATGTTATG+TGG | - | chr8.1:59159061-59159080 | MS.gene067247:CDS | 35.0% |
| ! | GGTTTAGACGTTTTGTCTAT+TGG | - | chr8.1:59159161-59159180 | MS.gene067247:CDS | 35.0% |
| ! | TGTGCATTCTTGTTTGTCTT+TGG | + | chr8.1:59158096-59158115 | None:intergenic | 35.0% |
| ! | TTTTCCTTCACCTTCAACAT+AGG | + | chr8.1:59158150-59158169 | None:intergenic | 35.0% |
| !!! | TGTCTATTGGGGTTTTAGTT+TGG | - | chr8.1:59159174-59159193 | MS.gene067247:CDS | 35.0% |
| AAGCGAAGTTTCATACCTGA+TGG | + | chr8.1:59158667-59158686 | None:intergenic | 40.0% | |
| AGCACTTGGACTAATATTGC+TGG | + | chr8.1:59158635-59158654 | None:intergenic | 40.0% | |
| CTACCACAACACAAAAGTCA+TGG | - | chr8.1:59158016-59158035 | MS.gene067247:CDS | 40.0% | |
| GAATGCACAGCTCAATTAAC+AGG | - | chr8.1:59158107-59158126 | MS.gene067247:CDS | 40.0% | |
| GCATCAATGATAGTTGGGAT+TGG | - | chr8.1:59158056-59158075 | MS.gene067247:CDS | 40.0% | |
| GCCAATATCTCTAAATGTCC+TGG | - | chr8.1:59158329-59158348 | MS.gene067247:intron | 40.0% | |
| GGACATTTAGAGATATTGGC+AGG | + | chr8.1:59158329-59158348 | None:intergenic | 40.0% | |
| TCATGTTATGTGGCTGTAGA+TGG | - | chr8.1:59159071-59159090 | MS.gene067247:CDS | 40.0% | |
| TCTGTGTAGTACTTCTTGCT+TGG | + | chr8.1:59159106-59159125 | None:intergenic | 40.0% | |
| TTCCTTCACCTTCAACATAG+GGG | + | chr8.1:59158148-59158167 | None:intergenic | 40.0% | |
| TTGAGACCATCACAACAATC+TGG | + | chr8.1:59158182-59158201 | None:intergenic | 40.0% | |
| !! | AGTTTCATACCTGATGGACT+AGG | + | chr8.1:59158661-59158680 | None:intergenic | 40.0% |
| !!! | TTTTATTTGGCTCTGCTGCT+GGG | - | chr8.1:59159198-59159217 | MS.gene067247:CDS | 40.0% |
| !!! | TTTTTATTTGGCTCTGCTGC+TGG | - | chr8.1:59159197-59159216 | MS.gene067247:CDS | 40.0% |
| AACTTGAGCCTCAGCTGATT+TGG | + | chr8.1:59158590-59158609 | None:intergenic | 45.0% | |
| AAGCTCAGGAGAACTGATCA+TGG | - | chr8.1:59158498-59158517 | MS.gene067247:intron | 45.0% | |
| AGCTCAGGAGAACTGATCAT+GGG | - | chr8.1:59158499-59158518 | MS.gene067247:intron | 45.0% | |
| TCATGTCTCCCCTATGTTGA+AGG | - | chr8.1:59158137-59158156 | MS.gene067247:CDS | 45.0% | |
| TCTCCTGAGCTTAACTCAGT+TGG | + | chr8.1:59158490-59158509 | None:intergenic | 45.0% | |
| TGAATTAGAGCTGCTGCACA+TGG | - | chr8.1:59158561-59158580 | MS.gene067247:intron | 45.0% | |
| ! | ACTTGAGCCTCAGCTGATTT+GGG | + | chr8.1:59158589-59158608 | None:intergenic | 45.0% |
| ! | GAGCCATGACTTTTGTGTTG+TGG | + | chr8.1:59158022-59158041 | None:intergenic | 45.0% |
| !! | CGTAATGATCCTGATTTGGG+TGG | - | chr8.1:59158251-59158270 | MS.gene067247:intron | 45.0% |
| !!! | ATATAAAACAATTTTATAAT+AGG | + | chr8.1:59158799-59158818 | None:intergenic | 5.0% |
| CATGGATCCCAAATCAGCTG+AGG | - | chr8.1:59158579-59158598 | MS.gene067247:CDS | 50.0% | |
| CCAGCACCAGATTGTTGTGA+TGG | - | chr8.1:59158173-59158192 | MS.gene067247:intron | 50.0% | |
| CCATCACAACAATCTGGTGC+TGG | + | chr8.1:59158176-59158195 | None:intergenic | 50.0% | |
| CTCCCCTATGTTGAAGGTGA+AGG | - | chr8.1:59158143-59158162 | MS.gene067247:intron | 50.0% | |
| CTGCCAACTGAGTTAAGCTC+AGG | - | chr8.1:59158484-59158503 | MS.gene067247:intron | 50.0% | |
| TGGTAGTCGGGGTTTGAACT+CGG | - | chr8.1:59158436-59158455 | MS.gene067247:intron | 50.0% | |
| !! | GCAGGGGCATTACAAACTGT+TGG | + | chr8.1:59158311-59158330 | None:intergenic | 50.0% |
| AGTGCTGCACCTAGTCCATC+AGG | - | chr8.1:59158649-59158668 | MS.gene067247:CDS | 55.0% | |
| GGTAGTCGGGGTTTGAACTC+GGG | - | chr8.1:59158437-59158456 | MS.gene067247:intron | 55.0% | |
| !! | GATGGACTAGGTGCAGCACT+TGG | + | chr8.1:59158649-59158668 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 59158011 | 59159235 | 59158011 | ID=MS.gene067247 |
| chr8.1 | mRNA | 59158011 | 59159235 | 59158011 | ID=MS.gene067247.t1;Parent=MS.gene067247 |
| chr8.1 | exon | 59158896 | 59159235 | 59158896 | ID=MS.gene067247.t1.exon1;Parent=MS.gene067247.t1 |
| chr8.1 | CDS | 59158896 | 59159235 | 59158896 | ID=cds.MS.gene067247.t1;Parent=MS.gene067247.t1 |
| chr8.1 | exon | 59158576 | 59158677 | 59158576 | ID=MS.gene067247.t1.exon2;Parent=MS.gene067247.t1 |
| chr8.1 | CDS | 59158576 | 59158677 | 59158576 | ID=cds.MS.gene067247.t1;Parent=MS.gene067247.t1 |
| chr8.1 | exon | 59158011 | 59158156 | 59158011 | ID=MS.gene067247.t1.exon3;Parent=MS.gene067247.t1 |
| chr8.1 | CDS | 59158011 | 59158156 | 59158011 | ID=cds.MS.gene067247.t1;Parent=MS.gene067247.t1 |
| Gene Sequence |
| Protein sequence |
