Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016280.t1 | XP_013445286.1 | 95.5 | 178 | 8 | 0 | 1 | 178 | 18 | 195 | 2.50E-91 | 344.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016280.t1 | O64864 | 44.6 | 184 | 86 | 3 | 1 | 169 | 20 | 202 | 9.4e-39 | 161.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016280.t1 | A0A072TPI6 | 95.5 | 178 | 8 | 0 | 1 | 178 | 18 | 195 | 1.8e-91 | 344.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene016280 | MS.gene77500 | PPI |
| MS.gene016280 | MS.gene09303 | PPI |
| MS.gene023520 | MS.gene016280 | PPI |
| MS.gene77503 | MS.gene016280 | PPI |
| MS.gene75704 | MS.gene016280 | PPI |
| MS.gene016280 | MS.gene08785 | PPI |
| MS.gene08939 | MS.gene016280 | PPI |
| MS.gene79864 | MS.gene016280 | PPI |
| MS.gene016280 | MS.gene045389 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016280.t1 | MTR_8g446830 | 95.506 | 178 | 8 | 0 | 1 | 178 | 18 | 195 | 3.54e-126 | 353 |
| MS.gene016280.t1 | MTR_7g014880 | 49.153 | 177 | 77 | 5 | 1 | 168 | 19 | 191 | 3.36e-50 | 160 |
| MS.gene016280.t1 | MTR_8g085560 | 41.143 | 175 | 95 | 4 | 1 | 169 | 20 | 192 | 1.77e-39 | 133 |
| MS.gene016280.t1 | MTR_3g083760 | 51.304 | 115 | 55 | 1 | 4 | 118 | 18 | 131 | 4.72e-36 | 122 |
| MS.gene016280.t1 | MTR_3g083760 | 44.697 | 132 | 55 | 2 | 4 | 118 | 18 | 148 | 1.43e-31 | 113 |
| MS.gene016280.t1 | MTR_6g084630 | 37.963 | 108 | 59 | 5 | 28 | 130 | 1 | 105 | 5.56e-13 | 63.2 |
| MS.gene016280.t1 | MTR_3g085210 | 33.333 | 114 | 72 | 3 | 1 | 111 | 17 | 129 | 4.75e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016280.t1 | AT2G44300 | 50.568 | 176 | 71 | 3 | 9 | 169 | 27 | 201 | 6.26e-58 | 180 |
| MS.gene016280.t1 | AT2G44290 | 45.652 | 184 | 84 | 3 | 1 | 169 | 20 | 202 | 3.16e-55 | 173 |
| MS.gene016280.t1 | AT1G55260 | 35.882 | 170 | 105 | 4 | 1 | 169 | 60 | 226 | 1.89e-33 | 119 |
| MS.gene016280.t1 | AT1G55260 | 37.059 | 170 | 100 | 5 | 1 | 169 | 60 | 223 | 3.38e-33 | 118 |
| MS.gene016280.t1 | AT3G58550 | 40.152 | 132 | 75 | 3 | 1 | 130 | 23 | 152 | 1.38e-27 | 102 |
| MS.gene016280.t1 | AT1G73890 | 40.506 | 79 | 46 | 1 | 17 | 95 | 30 | 107 | 7.15e-13 | 64.3 |
| MS.gene016280.t1 | AT1G03103 | 30.065 | 153 | 94 | 4 | 17 | 165 | 25 | 168 | 1.98e-11 | 60.1 |
Find 34 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTACTAGCATCAATGATAAT+TGG | 0.174596 | 8.2:-55753331 | None:intergenic |
| AACTTGAGCCTCAGCTGATT+TGG | 0.222383 | 8.2:+55752798 | None:intergenic |
| TGTGCATTCTTGTTTGTCTT+TGG | 0.244832 | 8.2:+55753288 | None:intergenic |
| ACCAGGACATTTAGAGATAT+TGG | 0.273723 | 8.2:+55753051 | None:intergenic |
| GAATGCACAGCTCAATTAAC+AGG | 0.295795 | 8.2:-55753274 | MS.gene016280:CDS |
| AGATCGTAATGATCCTGATT+TGG | 0.316207 | 8.2:-55753134 | MS.gene016280:CDS |
| AGAAAAGAGAAGAGATTGAT+TGG | 0.339081 | 8.2:-55752245 | MS.gene016280:CDS |
| ACTTGAGCCTCAGCTGATTT+GGG | 0.345043 | 8.2:+55752799 | None:intergenic |
| TACTAGCATCAATGATAATT+GGG | 0.392060 | 8.2:-55753330 | None:intergenic |
| GATCGTAATGATCCTGATTT+GGG | 0.411730 | 8.2:-55753133 | MS.gene016280:CDS |
| TATAGTTATTAATGTTACTT+TGG | 0.452178 | 8.2:-55753107 | MS.gene016280:CDS |
| TCTGTGTAGTACTTCTTGCT+TGG | 0.453220 | 8.2:+55752282 | None:intergenic |
| TCATGTCTCCCCTATGTTGA+AGG | 0.464755 | 8.2:-55753244 | MS.gene016280:CDS |
| GCCAATATCTCTAAATGTCC+TGG | 0.474666 | 8.2:-55753052 | MS.gene016280:intron |
| TTTCCTTCACCTTCAACATA+GGG | 0.478002 | 8.2:+55753235 | None:intergenic |
| GGACATTTAGAGATATTGGC+AGG | 0.478911 | 8.2:+55753055 | None:intergenic |
| ATAACTATACCACCCAAATC+AGG | 0.482338 | 8.2:+55753121 | None:intergenic |
| AGTGCTGCACCTAGTCCATC+AGG | 0.483330 | 8.2:-55752736 | MS.gene016280:intron |
| AGCACTTGGACTAATATTGC+TGG | 0.486730 | 8.2:+55752753 | None:intergenic |
| GCATCAATGATAATTGGGAT+TGG | 0.502649 | 8.2:-55753325 | None:intergenic |
| TGAATTAGAGCTGCTGCACA+TGG | 0.506732 | 8.2:-55752824 | MS.gene016280:intron |
| TTGAGACCATCACAACAATC+TGG | 0.516643 | 8.2:+55753202 | None:intergenic |
| GCAGGGGCATTACAAACTGT+TGG | 0.517485 | 8.2:+55753073 | None:intergenic |
| CCATCACAACAATCTGGTGC+TGG | 0.553657 | 8.2:+55753208 | None:intergenic |
| AGTTTCATACCTGATGGACT+AGG | 0.561670 | 8.2:+55752727 | None:intergenic |
| CTCCCCTATGTTGAAGGTGA+AGG | 0.561730 | 8.2:-55753238 | MS.gene016280:CDS |
| CCAGCACCAGATTGTTGTGA+TGG | 0.573645 | 8.2:-55753208 | MS.gene016280:CDS |
| ACATTTAGAGATATTGGCAG+GGG | 0.578847 | 8.2:+55753057 | None:intergenic |
| GATGGACTAGGTGCAGCACT+TGG | 0.582516 | 8.2:+55752739 | None:intergenic |
| GACATTTAGAGATATTGGCA+GGG | 0.601482 | 8.2:+55753056 | None:intergenic |
| TTCCTTCACCTTCAACATAG+GGG | 0.609504 | 8.2:+55753236 | None:intergenic |
| CGTAATGATCCTGATTTGGG+TGG | 0.647273 | 8.2:-55753130 | MS.gene016280:CDS |
| CATGGATCCCAAATCAGCTG+AGG | 0.670451 | 8.2:-55752806 | MS.gene016280:CDS |
| AATGCACAGCTCAATTAACA+GGG | 0.714051 | 8.2:-55753273 | MS.gene016280:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATTCAATAATTTTTAG+AGG | - | chr8.2:55753056-55753075 | MS.gene016280:CDS | 10.0% |
| !! | AATGACAAATAATAAAATTG+GGG | - | chr8.2:55753110-55753129 | MS.gene016280:CDS | 15.0% |
| !! | CAATGACAAATAATAAAATT+GGG | - | chr8.2:55753109-55753128 | MS.gene016280:CDS | 15.0% |
| !! | TAAATTATTGCATATTTGTT+TGG | + | chr8.2:55753001-55753020 | None:intergenic | 15.0% |
| !! | TATGTATATATAAATTGTAG+AGG | + | chr8.2:55752867-55752886 | None:intergenic | 15.0% |
| !! | TCAATGACAAATAATAAAAT+TGG | - | chr8.2:55753108-55753127 | MS.gene016280:CDS | 15.0% |
| !!! | GTTTTTTATCAATTAAACAA+TGG | - | chr8.2:55752713-55752732 | MS.gene016280:intron | 15.0% |
| !!! | TATAGTTATTAATGTTACTT+TGG | - | chr8.2:55752384-55752403 | MS.gene016280:intron | 15.0% |
| !!! | TTATTTCTCAATAATTTTAG+AGG | - | chr8.2:55753018-55753037 | MS.gene016280:intron | 15.0% |
| !! | AAATGAGTCAAATTGATTAT+GGG | - | chr8.2:55752506-55752525 | MS.gene016280:intron | 20.0% |
| !! | ATGACAAATAATAAAATTGG+GGG | - | chr8.2:55753111-55753130 | MS.gene016280:CDS | 20.0% |
| !! | ATTAGCAAAAAATCACAATT+AGG | + | chr8.2:55753146-55753165 | None:intergenic | 20.0% |
| !! | GGATATAACATAACATTAAA+AGG | - | chr8.2:55752626-55752645 | MS.gene016280:intron | 20.0% |
| !! | TTAGCAAAAAATCACAATTA+GGG | + | chr8.2:55753145-55753164 | None:intergenic | 20.0% |
| !!! | GTTTTAGTTTGGTTTTTATT+TGG | - | chr8.2:55753291-55753310 | MS.gene016280:CDS | 20.0% |
| !!! | TTGATTATGGGATTTTTTTT+TGG | - | chr8.2:55752518-55752537 | MS.gene016280:intron | 20.0% |
| ! | GAAATGAGTCAAATTGATTA+TGG | - | chr8.2:55752505-55752524 | MS.gene016280:intron | 25.0% |
| ! | TAACATAGAAAAACTTTACC+AGG | + | chr8.2:55752460-55752479 | None:intergenic | 25.0% |
| ! | TGACAAATAATAAAATTGGG+GGG | - | chr8.2:55753112-55753131 | MS.gene016280:CDS | 25.0% |
| !!! | ATTTTTTTTTGGTTGGTAGT+CGG | - | chr8.2:55752529-55752548 | MS.gene016280:intron | 25.0% |
| !!! | TTATGGGATTTTTTTTTGGT+TGG | - | chr8.2:55752522-55752541 | MS.gene016280:intron | 25.0% |
| AGAAAAGAGAAGAGATTGAT+TGG | - | chr8.2:55753246-55753265 | MS.gene016280:CDS | 30.0% | |
| ! | GTTTAGACGTTTTGTCTATT+GGG | - | chr8.2:55753268-55753287 | MS.gene016280:CDS | 30.0% |
| ! | TTTAGACGTTTTGTCTATTG+GGG | - | chr8.2:55753269-55753288 | MS.gene016280:CDS | 30.0% |
| !!! | TTTTTTTTTGGTTGGTAGTC+GGG | - | chr8.2:55752530-55752549 | MS.gene016280:intron | 30.0% |
| AATGCACAGCTCAATTAACA+GGG | - | chr8.2:55752218-55752237 | MS.gene016280:CDS | 35.0% | |
| ACATTTAGAGATATTGGCAG+GGG | + | chr8.2:55752437-55752456 | None:intergenic | 35.0% | |
| ACCAGGACATTTAGAGATAT+TGG | + | chr8.2:55752443-55752462 | None:intergenic | 35.0% | |
| AGATCGTAATGATCCTGATT+TGG | - | chr8.2:55752357-55752376 | MS.gene016280:intron | 35.0% | |
| ATAACTATACCACCCAAATC+AGG | + | chr8.2:55752373-55752392 | None:intergenic | 35.0% | |
| GACATTTAGAGATATTGGCA+GGG | + | chr8.2:55752438-55752457 | None:intergenic | 35.0% | |
| GATCGTAATGATCCTGATTT+GGG | - | chr8.2:55752358-55752377 | MS.gene016280:intron | 35.0% | |
| TTTCCTTCACCTTCAACATA+GGG | + | chr8.2:55752259-55752278 | None:intergenic | 35.0% | |
| ! | AGAGTGTCATTCATGTTATG+TGG | - | chr8.2:55753167-55753186 | MS.gene016280:CDS | 35.0% |
| ! | GGTTTAGACGTTTTGTCTAT+TGG | - | chr8.2:55753267-55753286 | MS.gene016280:CDS | 35.0% |
| ! | TGTGCATTCTTGTTTGTCTT+TGG | + | chr8.2:55752206-55752225 | None:intergenic | 35.0% |
| ! | TTTTCCTTCACCTTCAACAT+AGG | + | chr8.2:55752260-55752279 | None:intergenic | 35.0% |
| !!! | TGTCTATTGGGGTTTTAGTT+TGG | - | chr8.2:55753280-55753299 | MS.gene016280:CDS | 35.0% |
| !!! | TTTTTTTTGGTTGGTAGTCG+GGG | - | chr8.2:55752531-55752550 | MS.gene016280:intron | 35.0% |
| AAGCGAAGTTTCATACCTGA+TGG | + | chr8.2:55752773-55752792 | None:intergenic | 40.0% | |
| AGCACTTGGACTAATATTGC+TGG | + | chr8.2:55752741-55752760 | None:intergenic | 40.0% | |
| GAATGCACAGCTCAATTAAC+AGG | - | chr8.2:55752217-55752236 | MS.gene016280:CDS | 40.0% | |
| GCCAATATCTCTAAATGTCC+TGG | - | chr8.2:55752439-55752458 | MS.gene016280:intron | 40.0% | |
| GGACATTTAGAGATATTGGC+AGG | + | chr8.2:55752439-55752458 | None:intergenic | 40.0% | |
| TCATGTTATGTGGCTGTAGA+TGG | - | chr8.2:55753177-55753196 | MS.gene016280:CDS | 40.0% | |
| TCTGTGTAGTACTTCTTGCT+TGG | + | chr8.2:55753212-55753231 | None:intergenic | 40.0% | |
| TTCCTTCACCTTCAACATAG+GGG | + | chr8.2:55752258-55752277 | None:intergenic | 40.0% | |
| TTGAGACCATCACAACAATC+TGG | + | chr8.2:55752292-55752311 | None:intergenic | 40.0% | |
| !! | AGTTTCATACCTGATGGACT+AGG | + | chr8.2:55752767-55752786 | None:intergenic | 40.0% |
| !!! | TTTTATTTGGCTCTGCTGCT+GGG | - | chr8.2:55753304-55753323 | MS.gene016280:CDS | 40.0% |
| !!! | TTTTTATTTGGCTCTGCTGC+TGG | - | chr8.2:55753303-55753322 | MS.gene016280:CDS | 40.0% |
| AACTTGAGCCTCAGCTGATT+TGG | + | chr8.2:55752696-55752715 | None:intergenic | 45.0% | |
| AAGCTCAGGAGAACTGATCA+TGG | - | chr8.2:55752604-55752623 | MS.gene016280:intron | 45.0% | |
| AGCTCAGGAGAACTGATCAT+GGG | - | chr8.2:55752605-55752624 | MS.gene016280:intron | 45.0% | |
| TCATGTCTCCCCTATGTTGA+AGG | - | chr8.2:55752247-55752266 | MS.gene016280:CDS | 45.0% | |
| TCTCCTGAGCTTAACTCAGT+TGG | + | chr8.2:55752596-55752615 | None:intergenic | 45.0% | |
| TGAATTAGAGCTGCTGCACA+TGG | - | chr8.2:55752667-55752686 | MS.gene016280:intron | 45.0% | |
| ! | ACTTGAGCCTCAGCTGATTT+GGG | + | chr8.2:55752695-55752714 | None:intergenic | 45.0% |
| !! | CGTAATGATCCTGATTTGGG+TGG | - | chr8.2:55752361-55752380 | MS.gene016280:intron | 45.0% |
| !!! | ATATAAAACAATTTTATAAT+AGG | + | chr8.2:55752905-55752924 | None:intergenic | 5.0% |
| CATGGATCCCAAATCAGCTG+AGG | - | chr8.2:55752685-55752704 | MS.gene016280:intron | 50.0% | |
| CCAGCACCAGATTGTTGTGA+TGG | - | chr8.2:55752283-55752302 | MS.gene016280:CDS | 50.0% | |
| CCATCACAACAATCTGGTGC+TGG | + | chr8.2:55752286-55752305 | None:intergenic | 50.0% | |
| CTCCCCTATGTTGAAGGTGA+AGG | - | chr8.2:55752253-55752272 | MS.gene016280:CDS | 50.0% | |
| CTGCCAACTGAGTTAAGCTC+AGG | - | chr8.2:55752590-55752609 | MS.gene016280:intron | 50.0% | |
| TGGTAGTCGGGGTTTGAACT+CGG | - | chr8.2:55752542-55752561 | MS.gene016280:intron | 50.0% | |
| !! | GCAGGGGCATTACAAACTGT+TGG | + | chr8.2:55752421-55752440 | None:intergenic | 50.0% |
| AGTGCTGCACCTAGTCCATC+AGG | - | chr8.2:55752755-55752774 | MS.gene016280:CDS | 55.0% | |
| GGTAGTCGGGGTTTGAACTC+GGG | - | chr8.2:55752543-55752562 | MS.gene016280:intron | 55.0% | |
| !! | GATGGACTAGGTGCAGCACT+TGG | + | chr8.2:55752755-55752774 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 55752172 | 55753341 | 55752172 | ID=MS.gene016280 |
| chr8.2 | mRNA | 55752172 | 55753341 | 55752172 | ID=MS.gene016280.t1;Parent=MS.gene016280 |
| chr8.2 | exon | 55753053 | 55753341 | 55753053 | ID=MS.gene016280.t1.exon1;Parent=MS.gene016280.t1 |
| chr8.2 | CDS | 55753053 | 55753341 | 55753053 | ID=cds.MS.gene016280.t1;Parent=MS.gene016280.t1 |
| chr8.2 | exon | 55752737 | 55752838 | 55752737 | ID=MS.gene016280.t1.exon2;Parent=MS.gene016280.t1 |
| chr8.2 | CDS | 55752737 | 55752838 | 55752737 | ID=cds.MS.gene016280.t1;Parent=MS.gene016280.t1 |
| chr8.2 | exon | 55752172 | 55752317 | 55752172 | ID=MS.gene016280.t1.exon3;Parent=MS.gene016280.t1 |
| chr8.2 | CDS | 55752172 | 55752317 | 55752172 | ID=cds.MS.gene016280.t1;Parent=MS.gene016280.t1 |
| Gene Sequence |
| Protein sequence |
