Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59892.t1 | XP_013445286.1 | 94.1 | 187 | 11 | 0 | 1 | 187 | 9 | 195 | 3.30E-94 | 354.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59892.t1 | O64864 | 43.9 | 189 | 90 | 3 | 5 | 178 | 15 | 202 | 1.5e-39 | 164.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59892.t1 | A0A072TPI6 | 94.1 | 187 | 11 | 0 | 1 | 187 | 9 | 195 | 2.4e-94 | 354.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052494 | MS.gene59892 | 0.807026 | 6.18E-50 | -1.69E-46 |
| MS.gene055158 | MS.gene59892 | 0.808314 | 3.29E-50 | -1.69E-46 |
| MS.gene05960 | MS.gene59892 | 0.841758 | 3.91E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene59892 | MS.gene023520 | PPI |
| MS.gene77503 | MS.gene59892 | PPI |
| MS.gene75704 | MS.gene59892 | PPI |
| MS.gene59892 | MS.gene08785 | PPI |
| MS.gene08939 | MS.gene59892 | PPI |
| MS.gene59892 | MS.gene045389 | PPI |
| MS.gene59892 | MS.gene79864 | PPI |
| MS.gene59892 | MS.gene77500 | PPI |
| MS.gene59892 | MS.gene09303 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59892.t1 | MTR_8g446830 | 94.118 | 187 | 11 | 0 | 1 | 187 | 9 | 195 | 8.41e-131 | 365 |
| MS.gene59892.t1 | MTR_7g014880 | 48.387 | 186 | 83 | 5 | 1 | 177 | 10 | 191 | 5.27e-51 | 162 |
| MS.gene59892.t1 | MTR_8g085560 | 40.223 | 179 | 101 | 4 | 5 | 180 | 15 | 190 | 5.74e-41 | 137 |
| MS.gene59892.t1 | MTR_3g083760 | 51.304 | 115 | 55 | 1 | 13 | 127 | 18 | 131 | 6.94e-36 | 122 |
| MS.gene59892.t1 | MTR_3g083760 | 44.697 | 132 | 55 | 2 | 13 | 127 | 18 | 148 | 1.55e-31 | 113 |
| MS.gene59892.t1 | MTR_3g085210 | 32.231 | 121 | 78 | 3 | 4 | 120 | 9 | 129 | 3.19e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59892.t1 | AT2G44300 | 50.568 | 176 | 71 | 3 | 18 | 178 | 27 | 201 | 1.17e-57 | 180 |
| MS.gene59892.t1 | AT2G44290 | 44.974 | 189 | 88 | 3 | 5 | 178 | 15 | 202 | 2.45e-56 | 177 |
| MS.gene59892.t1 | AT1G55260 | 35.429 | 175 | 109 | 4 | 5 | 178 | 55 | 226 | 1.28e-34 | 122 |
| MS.gene59892.t1 | AT1G55260 | 36.571 | 175 | 104 | 5 | 5 | 178 | 55 | 223 | 1.87e-34 | 121 |
| MS.gene59892.t1 | AT3G58550 | 39.416 | 137 | 79 | 3 | 5 | 139 | 18 | 152 | 4.50e-28 | 103 |
| MS.gene59892.t1 | AT1G73890 | 40.506 | 79 | 46 | 1 | 26 | 104 | 30 | 107 | 9.81e-13 | 63.9 |
| MS.gene59892.t1 | AT1G03103 | 28.161 | 174 | 108 | 5 | 5 | 174 | 8 | 168 | 8.47e-12 | 61.2 |
Find 34 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTACTAGCATCAATGATAAT+TGG | 0.191995 | 8.3:-53211039 | MS.gene59892:CDS |
| AACTTGAGCCTCAGCTGATT+TGG | 0.222383 | 8.3:+53210510 | None:intergenic |
| ACCAGGACATTTAGAGATAT+TGG | 0.273723 | 8.3:+53210759 | None:intergenic |
| GAATGCACAGCTCAATTAAC+AGG | 0.295795 | 8.3:-53210982 | MS.gene59892:CDS |
| AGATCGTAATGATCCTGATT+TGG | 0.316207 | 8.3:-53210842 | MS.gene59892:CDS |
| AGAAAAGAGAAGAGATTGAT+TGG | 0.339081 | 8.3:-53209954 | MS.gene59892:CDS |
| ACTTGAGCCTCAGCTGATTT+GGG | 0.345043 | 8.3:+53210511 | None:intergenic |
| TACTAGCATCAATGATAATT+GGG | 0.397326 | 8.3:-53211038 | MS.gene59892:CDS |
| GATCGTAATGATCCTGATTT+GGG | 0.411730 | 8.3:-53210841 | MS.gene59892:CDS |
| TATAGTTATTAATGTTACTT+TGG | 0.452178 | 8.3:-53210815 | MS.gene59892:CDS |
| TCTGTGTAGTACTTCTTGCT+TGG | 0.453220 | 8.3:+53209991 | None:intergenic |
| TCATGTCTCCCCTATGTTGA+AGG | 0.464755 | 8.3:-53210952 | MS.gene59892:CDS |
| GCCAATATCTCTAAATGTCC+TGG | 0.474666 | 8.3:-53210760 | MS.gene59892:intron |
| TTTCCTTCACCTTCAACATA+GGG | 0.478002 | 8.3:+53210943 | None:intergenic |
| GGACATTTAGAGATATTGGC+AGG | 0.478911 | 8.3:+53210763 | None:intergenic |
| ATAACTATACCACCCAAATC+AGG | 0.482338 | 8.3:+53210829 | None:intergenic |
| AGTGCTGCACCTAGTCCATC+AGG | 0.483330 | 8.3:-53210448 | MS.gene59892:intron |
| AGCACTTGGACTAATATTGC+TGG | 0.486730 | 8.3:+53210465 | None:intergenic |
| GCATCAATGATAATTGGGAT+TGG | 0.502649 | 8.3:-53211033 | MS.gene59892:CDS |
| TGAATTAGAGCTGCTGCACA+TGG | 0.506732 | 8.3:-53210536 | MS.gene59892:intron |
| TTGAGACCATCACAACAATC+TGG | 0.516643 | 8.3:+53210910 | None:intergenic |
| GCAGGGGCATTACAAACTGT+TGG | 0.517485 | 8.3:+53210781 | None:intergenic |
| CCATCACAACAATCTGGTGC+TGG | 0.553657 | 8.3:+53210916 | None:intergenic |
| AGTTTCATACCTGATGGACT+AGG | 0.561670 | 8.3:+53210439 | None:intergenic |
| CTCCCCTATGTTGAAGGTGA+AGG | 0.561730 | 8.3:-53210946 | MS.gene59892:CDS |
| CCAGCACCAGATTGTTGTGA+TGG | 0.573645 | 8.3:-53210916 | MS.gene59892:CDS |
| ACATTTAGAGATATTGGCAG+GGG | 0.578847 | 8.3:+53210765 | None:intergenic |
| GATGGACTAGGTGCAGCACT+TGG | 0.582516 | 8.3:+53210451 | None:intergenic |
| GACATTTAGAGATATTGGCA+GGG | 0.601482 | 8.3:+53210764 | None:intergenic |
| TTCCTTCACCTTCAACATAG+GGG | 0.609504 | 8.3:+53210944 | None:intergenic |
| CGTAATGATCCTGATTTGGG+TGG | 0.647273 | 8.3:-53210838 | MS.gene59892:CDS |
| TAGTAGTAGAAAATGAGTCA+TGG | 0.656551 | 8.3:+53211056 | None:intergenic |
| CATGGATCCCAAATCAGCTG+AGG | 0.670451 | 8.3:-53210518 | MS.gene59892:CDS |
| AATGCACAGCTCAATTAACA+GGG | 0.714051 | 8.3:-53210981 | MS.gene59892:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATTCAATAATTTTTAG+AGG | - | chr8.3:53210792-53210811 | MS.gene59892:CDS | 10.0% |
| !! | AATGACAAATAATAAAATTG+TGG | - | chr8.3:53210844-53210863 | MS.gene59892:CDS | 15.0% |
| !! | ATGACAAATAATAAAATTGT+GGG | - | chr8.3:53210845-53210864 | MS.gene59892:CDS | 15.0% |
| !! | TAAATTATTGCATATTTGTT+TGG | + | chr8.3:53210735-53210754 | None:intergenic | 15.0% |
| !! | TATGTATATATAAATTGTAG+AGG | + | chr8.3:53210597-53210616 | None:intergenic | 15.0% |
| !!! | GTTTTTTATCAATTAAACAA+TGG | - | chr8.3:53210445-53210464 | MS.gene59892:intron | 15.0% |
| !!! | TATAGTTATTAATGTTACTT+TGG | - | chr8.3:53210120-53210139 | MS.gene59892:intron | 15.0% |
| !!! | TTATTTCTCAATAATTTTAG+AGG | - | chr8.3:53210752-53210771 | MS.gene59892:intron | 15.0% |
| !! | AACAATACATAATATATGCA+AGG | + | chr8.3:53210302-53210321 | None:intergenic | 20.0% |
| !! | ATTAGCAAAAAATCACAATT+AGG | + | chr8.3:53210881-53210900 | None:intergenic | 20.0% |
| !! | TGACAAATAATAAAATTGTG+GGG | - | chr8.3:53210846-53210865 | MS.gene59892:CDS | 20.0% |
| !! | TTAGCAAAAAATCACAATTA+GGG | + | chr8.3:53210880-53210899 | None:intergenic | 20.0% |
| !!! | GTTTTAGTTTGGTTTTTATT+TGG | - | chr8.3:53211026-53211045 | MS.gene59892:CDS | 20.0% |
| ! | AACTGAGTCAAATTGATTAT+GGG | - | chr8.3:53210242-53210261 | MS.gene59892:intron | 25.0% |
| ! | GACAAATAATAAAATTGTGG+GGG | - | chr8.3:53210847-53210866 | MS.gene59892:CDS | 25.0% |
| ! | GGGATATAACATAACATTAA+AGG | - | chr8.3:53210358-53210377 | MS.gene59892:intron | 25.0% |
| ! | TAACATAGAAAAACTTTACC+AGG | + | chr8.3:53210196-53210215 | None:intergenic | 25.0% |
| ! | TACTAGCATCAATGATAATT+GGG | - | chr8.3:53209897-53209916 | MS.gene59892:CDS | 25.0% |
| !!! | GATTTTTTTTTTAGTAGTCG+GGG | - | chr8.3:53210264-53210283 | MS.gene59892:intron | 25.0% |
| !!! | GGATTTTTTTTTTAGTAGTC+GGG | - | chr8.3:53210263-53210282 | MS.gene59892:intron | 25.0% |
| !!! | GGGATTTTTTTTTTAGTAGT+CGG | - | chr8.3:53210262-53210281 | MS.gene59892:intron | 25.0% |
| AGAAAAGAGAAGAGATTGAT+TGG | - | chr8.3:53210981-53211000 | MS.gene59892:CDS | 30.0% | |
| CTACTAGCATCAATGATAAT+TGG | - | chr8.3:53209896-53209915 | MS.gene59892:CDS | 30.0% | |
| GAACTGAGTCAAATTGATTA+TGG | - | chr8.3:53210241-53210260 | MS.gene59892:intron | 30.0% | |
| ! | GTTTAGACGTTTTGTCTATT+GGG | - | chr8.3:53211003-53211022 | MS.gene59892:CDS | 30.0% |
| ! | TTTAGACGTTTTGTCTATTG+GGG | - | chr8.3:53211004-53211023 | MS.gene59892:CDS | 30.0% |
| !! | TTTTTATTTGGTTATGCTGC+TGG | - | chr8.3:53211038-53211057 | MS.gene59892:CDS | 30.0% |
| AATGCACAGCTCAATTAACA+GGG | - | chr8.3:53209954-53209973 | MS.gene59892:CDS | 35.0% | |
| ACATTTAGAGATATTGGCAG+GGG | + | chr8.3:53210173-53210192 | None:intergenic | 35.0% | |
| ACCAGGACATTTAGAGATAT+TGG | + | chr8.3:53210179-53210198 | None:intergenic | 35.0% | |
| AGATCGTAATGATCCTGATT+TGG | - | chr8.3:53210093-53210112 | MS.gene59892:intron | 35.0% | |
| ATAACTATACCACCCAAATC+AGG | + | chr8.3:53210109-53210128 | None:intergenic | 35.0% | |
| GACATTTAGAGATATTGGCA+GGG | + | chr8.3:53210174-53210193 | None:intergenic | 35.0% | |
| GATCGTAATGATCCTGATTT+GGG | - | chr8.3:53210094-53210113 | MS.gene59892:intron | 35.0% | |
| GCATCAATGATAATTGGGAT+TGG | - | chr8.3:53209902-53209921 | MS.gene59892:CDS | 35.0% | |
| TTTCCTTCACCTTCAACATA+GGG | + | chr8.3:53209995-53210014 | None:intergenic | 35.0% | |
| ! | AGAGTGTCATTCATGTTATG+TGG | - | chr8.3:53210902-53210921 | MS.gene59892:CDS | 35.0% |
| ! | GGTTTAGACGTTTTGTCTAT+TGG | - | chr8.3:53211002-53211021 | MS.gene59892:CDS | 35.0% |
| ! | TTTTCCTTCACCTTCAACAT+AGG | + | chr8.3:53209996-53210015 | None:intergenic | 35.0% |
| !!! | TGTCTATTGGGGTTTTAGTT+TGG | - | chr8.3:53211015-53211034 | MS.gene59892:CDS | 35.0% |
| AAGCGAAGTTTCATACCTGA+TGG | + | chr8.3:53210505-53210524 | None:intergenic | 40.0% | |
| AAGCTCAAGAGAACTGATCA+TGG | - | chr8.3:53210337-53210356 | MS.gene59892:intron | 40.0% | |
| AGCACTTGGACTAATATTGC+TGG | + | chr8.3:53210473-53210492 | None:intergenic | 40.0% | |
| AGCTCAAGAGAACTGATCAT+GGG | - | chr8.3:53210338-53210357 | MS.gene59892:intron | 40.0% | |
| GAATGCACAGCTCAATTAAC+AGG | - | chr8.3:53209953-53209972 | MS.gene59892:CDS | 40.0% | |
| GCCAATATCTCTAAATGTCC+TGG | - | chr8.3:53210175-53210194 | MS.gene59892:intron | 40.0% | |
| GGACATTTAGAGATATTGGC+AGG | + | chr8.3:53210175-53210194 | None:intergenic | 40.0% | |
| TCATGTTATGTGGCTGTAGA+TGG | - | chr8.3:53210912-53210931 | MS.gene59892:CDS | 40.0% | |
| TCTCTTGAGCTTAACTCAGT+TGG | + | chr8.3:53210329-53210348 | None:intergenic | 40.0% | |
| TCTGTGTAGTACTTCTTGCT+TGG | + | chr8.3:53210947-53210966 | None:intergenic | 40.0% | |
| TTCCTTCACCTTCAACATAG+GGG | + | chr8.3:53209994-53210013 | None:intergenic | 40.0% | |
| TTGAGACCATCACAACAATC+TGG | + | chr8.3:53210028-53210047 | None:intergenic | 40.0% | |
| !! | AGTTTCATACCTGATGGACT+AGG | + | chr8.3:53210499-53210518 | None:intergenic | 40.0% |
| AACTTGAGCCTCAGCTGATT+TGG | + | chr8.3:53210428-53210447 | None:intergenic | 45.0% | |
| TAGTAGTCGGGGTTTGAACT+CGG | - | chr8.3:53210275-53210294 | MS.gene59892:intron | 45.0% | |
| TCATGTCTCCCCTATGTTGA+AGG | - | chr8.3:53209983-53210002 | MS.gene59892:CDS | 45.0% | |
| TGAATTAGAGCTGCTGCACA+TGG | - | chr8.3:53210399-53210418 | MS.gene59892:intron | 45.0% | |
| ! | ACTTGAGCCTCAGCTGATTT+GGG | + | chr8.3:53210427-53210446 | None:intergenic | 45.0% |
| !! | CGTAATGATCCTGATTTGGG+TGG | - | chr8.3:53210097-53210116 | MS.gene59892:intron | 45.0% |
| !!! | ATATAAAACAATTTTATAAT+AGG | + | chr8.3:53210635-53210654 | None:intergenic | 5.0% |
| CATGGATCCCAAATCAGCTG+AGG | - | chr8.3:53210417-53210436 | MS.gene59892:intron | 50.0% | |
| CCAGCACCAGATTGTTGTGA+TGG | - | chr8.3:53210019-53210038 | MS.gene59892:intron | 50.0% | |
| CCATCACAACAATCTGGTGC+TGG | + | chr8.3:53210022-53210041 | None:intergenic | 50.0% | |
| CTCCCCTATGTTGAAGGTGA+AGG | - | chr8.3:53209989-53210008 | MS.gene59892:CDS | 50.0% | |
| !! | GCAGGGGCATTACAAACTGT+TGG | + | chr8.3:53210157-53210176 | None:intergenic | 50.0% |
| AGTGCTGCACCTAGTCCATC+AGG | - | chr8.3:53210487-53210506 | MS.gene59892:CDS | 55.0% | |
| !! | GATGGACTAGGTGCAGCACT+TGG | + | chr8.3:53210487-53210506 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 53209881 | 53211076 | 53209881 | ID=MS.gene59892 |
| chr8.3 | mRNA | 53209881 | 53211076 | 53209881 | ID=MS.gene59892.t1;Parent=MS.gene59892 |
| chr8.3 | exon | 53210761 | 53211076 | 53210761 | ID=MS.gene59892.t1.exon1;Parent=MS.gene59892.t1 |
| chr8.3 | CDS | 53210761 | 53211076 | 53210761 | ID=cds.MS.gene59892.t1;Parent=MS.gene59892.t1 |
| chr8.3 | exon | 53210449 | 53210550 | 53210449 | ID=MS.gene59892.t1.exon2;Parent=MS.gene59892.t1 |
| chr8.3 | CDS | 53210449 | 53210550 | 53210449 | ID=cds.MS.gene59892.t1;Parent=MS.gene59892.t1 |
| chr8.3 | exon | 53209881 | 53210026 | 53209881 | ID=MS.gene59892.t1.exon3;Parent=MS.gene59892.t1 |
| chr8.3 | CDS | 53209881 | 53210026 | 53209881 | ID=cds.MS.gene59892.t1;Parent=MS.gene59892.t1 |
| Gene Sequence |
| Protein sequence |
