AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene048767

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048767.t1 MTR_8g096950 97.173 283 7 1 1 283 1 282 0.0 562
MS.gene048767.t1 MTR_5g010620 74.113 282 58 2 1 282 1 267 7.14e-149 417
MS.gene048767.t1 MTR_5g010620 74.113 282 58 2 1 282 33 299 1.13e-148 418
MS.gene048767.t1 MTR_3g110015 88.235 85 9 1 1 84 1 85 1.69e-44 146
MS.gene048767.t1 MTR_3g109978 88.235 85 9 1 1 84 92 176 7.18e-44 147
MS.gene048767.t1 MTR_7g055620 61.194 134 13 2 1 134 166 260 2.82e-42 149
MS.gene048767.t1 MTR_0411s0050 83.929 56 9 0 88 143 15 70 5.57e-27 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048767.t1 AT4G20330 66.312 282 92 2 1 282 1 279 9.37e-124 355
MS.gene048767.t1 AT4G21010 58.363 281 109 5 1 280 1 274 2.81e-96 285

Find 63 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCCGTCGTATTCTACTTT+TGG 0.214350 4.3:+6459975 None:intergenic
TCCACCTTAATGTTTGCTTT+GGG 0.248514 4.3:+6458974 None:intergenic
ATCCACCTTAATGTTTGCTT+TGG 0.267923 4.3:+6458973 None:intergenic
GCAGCCACCACCGGAGTATT+AGG 0.268237 4.3:+6461024 None:intergenic
CAGGAGTTGTTGTTTACTTT+AGG 0.277978 4.3:+6458717 None:intergenic
TGAAAGCTGCAGGGCAGATT+TGG 0.288630 4.3:-6459045 MS.gene048767:CDS
ATGTTTGCTTTGGGGTCATT+CGG 0.320738 4.3:+6458983 None:intergenic
CTCTTTGCAGTGTTGGTAGC+AGG 0.337273 4.3:+6458863 None:intergenic
CATGCTTCATTTATTTGCTC+TGG 0.343949 4.3:+6460050 None:intergenic
CAATGTGATGGAAGACTTGC+AGG 0.356725 4.3:-6459746 MS.gene048767:intron
ATTGGTCAGCTTGGTTCTCT+TGG 0.364216 4.3:+6458766 None:intergenic
CGCTTCATCTGAGCACCAAC+GGG 0.368774 4.3:+6460907 None:intergenic
CATTGGGGTAAGCATCCTTT+AGG 0.372124 4.3:+6459764 None:intergenic
CTTAGACTTGGACTTGGACT+TGG 0.379977 4.3:+6458802 None:intergenic
TGTTTGCTTTGGGGTCATTC+GGG 0.391698 4.3:+6458984 None:intergenic
TGCAAGTCTTCCATCACATT+GGG 0.401716 4.3:+6459748 None:intergenic
GCTGACCAATTCCCATCTTC+CGG 0.404549 4.3:-6458753 MS.gene048767:CDS
TGACCCCAAAGCAAACATTA+AGG 0.415737 4.3:-6458978 MS.gene048767:CDS
TTGTAGGCTTTGAAAGCTGC+AGG 0.415846 4.3:-6459055 MS.gene048767:intron
ATGACCTAAAGCAGCTGTTT+CGG 0.418120 4.3:-6458949 MS.gene048767:CDS
GAGAAGGATCTTCAAAAGAA+TGG 0.418846 4.3:-6458893 MS.gene048767:CDS
ATAGGCAAAGTATGGTCTTA+AGG 0.421161 4.3:-6459845 MS.gene048767:intron
AAGCTCCGGAAGATGGGAAT+TGG 0.433158 4.3:+6458748 None:intergenic
CTTGGACTTGGACTTGGACT+TGG 0.442430 4.3:+6458808 None:intergenic
TACTCCGAAACAGCTGCTTT+AGG 0.444378 4.3:+6458945 None:intergenic
AGCATTGCGTCGAGCAAAGT+TGG 0.452004 4.3:-6461057 MS.gene048767:CDS
AGTCCTCCTCTTTGCAGTGT+TGG 0.455410 4.3:+6458856 None:intergenic
GATTTCATTCTTCTTAGACT+TGG 0.459783 4.3:+6458790 None:intergenic
CCACCTTAATGTTTGCTTTG+GGG 0.471247 4.3:+6458975 None:intergenic
GCTGTCCAACTTTGATTCAC+AGG 0.488075 4.3:-6459020 MS.gene048767:CDS
AGGACTGCAGCACAAATTGA+TGG 0.492183 4.3:-6458839 MS.gene048767:CDS
GATGCATTTGCAGCCACCAC+CGG 0.494981 4.3:+6461015 None:intergenic
GCTGCAAATGCATCAGCAAA+TGG 0.504031 4.3:-6461006 MS.gene048767:CDS
AAGCATGCTATGTTGATATG+AGG 0.505702 4.3:-6460034 MS.gene048767:CDS
GTCGTCCTGTGAATCAAAGT+TGG 0.513690 4.3:+6459015 None:intergenic
ATTCTTCTTAGACTTGGACT+TGG 0.515148 4.3:+6458796 None:intergenic
CTGCAAGTCTTCCATCACAT+TGG 0.523283 4.3:+6459747 None:intergenic
CTCTGCAAGAGCAACTAGAT+AGG 0.523289 4.3:-6461119 MS.gene048767:CDS
AAGTAGAATACGACGGGCAA+CGG 0.531226 4.3:-6459971 MS.gene048767:CDS
CGGGCAACGGTTCTCTTACA+AGG 0.541030 4.3:-6459958 MS.gene048767:intron
AAGATGGGAATTGGTCAGCT+TGG 0.557060 4.3:+6458757 None:intergenic
TGTTATTGATCTATTGCTTG+AGG 0.558507 4.3:-6460884 MS.gene048767:intron
ACCCAAAAGTAGAATACGAC+GGG 0.561755 4.3:-6459977 MS.gene048767:CDS
GGATGCTTACCCCAATGTGA+TGG 0.574607 4.3:-6459758 MS.gene048767:CDS
AAAACCTAATACTCCGGTGG+TGG 0.582600 4.3:-6461028 MS.gene048767:CDS
TAGCAGAAAACCTAATACTC+CGG 0.589094 4.3:-6461034 MS.gene048767:CDS
ACGCTTCATCTGAGCACCAA+CGG 0.595575 4.3:+6460906 None:intergenic
AGCTTCTTCAACTTATACGT+AGG 0.600973 4.3:-6459813 MS.gene048767:CDS
TGATATGATTGATATAGAGA+AGG 0.617718 4.3:-6458909 MS.gene048767:CDS
CCCCAAAGCAAACATTAAGG+TGG 0.623815 4.3:-6458975 MS.gene048767:CDS
AATTCTCAAGTGACACTGAG+AGG 0.626331 4.3:-6460960 MS.gene048767:CDS
AGCATGCTATGTTGATATGA+GGG 0.636991 4.3:-6460033 MS.gene048767:CDS
TGTAGGCTTTGAAAGCTGCA+GGG 0.643501 4.3:-6459054 MS.gene048767:intron
CTACCAACACTGCAAAGAGG+AGG 0.656479 4.3:-6458859 MS.gene048767:CDS
CTGCTACCAACACTGCAAAG+AGG 0.665956 4.3:-6458862 MS.gene048767:CDS
GCTTCATCTGAGCACCAACG+GGG 0.666699 4.3:+6460908 None:intergenic
ACTTATACGTAGGTATCCAG+AGG 0.677982 4.3:-6459803 MS.gene048767:CDS
CTTATACGTAGGTATCCAGA+GGG 0.688413 4.3:-6459802 MS.gene048767:CDS
AACCCAAAAGTAGAATACGA+CGG 0.689064 4.3:-6459978 MS.gene048767:CDS
GCAAGTCTTCCATCACATTG+GGG 0.693489 4.3:+6459749 None:intergenic
CTTCATCTGAGCACCAACGG+GGG 0.696908 4.3:+6460909 None:intergenic
CAGAAAACCTAATACTCCGG+TGG 0.697637 4.3:-6461031 MS.gene048767:CDS
TCTATATCAATCATATCACG+AGG 0.742182 4.3:+6458914 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAACCTTAATTTATAATAA+TGG + chr4.3:6460418-6460437 None:intergenic 10.0%
!! AATAAACAAAACAAATGAAT+AGG + chr4.3:6460548-6460567 None:intergenic 15.0%
!! ATTGAAACTAATAATATGTT+TGG - chr4.3:6459210-6459229 MS.gene048767:intron 15.0%
!! TTAATTTATAATAATGGCAA+CGG + chr4.3:6460412-6460431 None:intergenic 15.0%
!!! TTGTTTGTTTTTTCATTTAT+TGG - chr4.3:6459025-6459044 MS.gene048767:CDS 15.0%
!! AATTAAGTTCTCAATATGTT+AGG + chr4.3:6459352-6459371 None:intergenic 20.0%
!! ACTTAAATAGATGACATAAA+GGG - chr4.3:6459430-6459449 MS.gene048767:intron 20.0%
!! ATAAATTAAGGTTAATACTG+AGG - chr4.3:6460423-6460442 MS.gene048767:intron 20.0%
!! ATTAAGTTCTCAATATGTTA+GGG + chr4.3:6459351-6459370 None:intergenic 20.0%
!! GTTGCCATTATTATAAATTA+AGG - chr4.3:6460411-6460430 MS.gene048767:intron 20.0%
!! TTAATGTTTGTCTTGAAATA+TGG - chr4.3:6460645-6460664 MS.gene048767:intron 20.0%
!!! AAGAAGATCAAATGTTTTAT+GGG - chr4.3:6460311-6460330 MS.gene048767:intron 20.0%
!!! GTTATTTGTTTTTTGTCTAT+AGG - chr4.3:6459990-6460009 MS.gene048767:CDS 20.0%
!!! TATTAGTTTCAATTTGCAAT+TGG + chr4.3:6459203-6459222 None:intergenic 20.0%
!!! TCATAATGATATCTTTTTGT+AGG - chr4.3:6460782-6460801 MS.gene048767:intron 20.0%
! AAACTAATAATATGTTTGGC+TGG - chr4.3:6459214-6459233 MS.gene048767:intron 25.0%
! AACATTTGATCTTCTTCAAA+AGG + chr4.3:6460307-6460326 None:intergenic 25.0%
! AATGGAAGAAATTCAAAAAC+TGG - chr4.3:6458865-6458884 MS.gene048767:CDS 25.0%
! ACATTTGATCTTCTTCAAAA+GGG + chr4.3:6460306-6460325 None:intergenic 25.0%
! ATAATAACATTGCACATTAG+TGG - chr4.3:6460338-6460357 MS.gene048767:intron 25.0%
! ATACTCTTAATCAATGTTCA+CGG + chr4.3:6459544-6459563 None:intergenic 25.0%
! ATGGAAATAGAGATTATAGA+TGG - chr4.3:6460664-6460683 MS.gene048767:intron 25.0%
! GACTTAAATAGATGACATAA+AGG - chr4.3:6459429-6459448 MS.gene048767:intron 25.0%
! GTCAAAATCTTGTATATAGT+TGG - chr4.3:6460740-6460759 MS.gene048767:intron 25.0%
! TAATAACATTGCACATTAGT+GGG - chr4.3:6460339-6460358 MS.gene048767:intron 25.0%
! TACATATCACTTATGTAGAA+TGG - chr4.3:6459491-6459510 MS.gene048767:intron 25.0%
! TGATATGATTGATATAGAGA+AGG - chr4.3:6460944-6460963 MS.gene048767:CDS 25.0%
! TTTCATTTATTGGAAGTTTC+TGG - chr4.3:6459035-6459054 MS.gene048767:CDS 25.0%
!! ATGAAGAAGTTATTTGCTAA+AGG + chr4.3:6459968-6459987 None:intergenic 25.0%
!! GAAGAAGATCAAATGTTTTA+TGG - chr4.3:6460310-6460329 MS.gene048767:intron 25.0%
AAAAACATCACCTATCACTA+AGG + chr4.3:6460707-6460726 None:intergenic 30.0%
AATTCTCATTTCTCACAGAT+TGG - chr4.3:6460229-6460248 MS.gene048767:intron 30.0%
ACCAAAACACCAAATCAATA+TGG + chr4.3:6459755-6459774 None:intergenic 30.0%
ACCAATGAAATTCCACAATT+AGG - chr4.3:6459713-6459732 MS.gene048767:intron 30.0%
AGATTATAGATGGAACTATC+AGG - chr4.3:6460674-6460693 MS.gene048767:intron 30.0%
CATCAAACTCCAAAATATCT+TGG + chr4.3:6459623-6459642 None:intergenic 30.0%
GATTTCATTCTTCTTAGACT+TGG + chr4.3:6461066-6461085 None:intergenic 30.0%
GTTTGCAAATTAAGCATACA+AGG - chr4.3:6459397-6459416 MS.gene048767:intron 30.0%
TAACCTCAAACTTAACAGAT+GGG + chr4.3:6459155-6459174 None:intergenic 30.0%
TCCTAATTGTGGAATTTCAT+TGG + chr4.3:6459717-6459736 None:intergenic 30.0%
TCTATATCAATCATATCACG+AGG + chr4.3:6460942-6460961 None:intergenic 30.0%
TGTTATTGATCTATTGCTTG+AGG - chr4.3:6458969-6458988 MS.gene048767:CDS 30.0%
TTAACCTCAAACTTAACAGA+TGG + chr4.3:6459156-6459175 None:intergenic 30.0%
TTACAAAATCTTGCTCCTTA+AGG - chr4.3:6460472-6460491 MS.gene048767:intron 30.0%
! AGTCATCTTCCAAGATATTT+TGG - chr4.3:6459611-6459630 MS.gene048767:intron 30.0%
! CTATTGCTTGAGGTAATTTT+TGG - chr4.3:6458979-6458998 MS.gene048767:CDS 30.0%
! CTCTTACAAGGTAACTTTTA+CGG - chr4.3:6459907-6459926 MS.gene048767:intron 30.0%
! TAAGGAGCAAGATTTTGTAA+TGG + chr4.3:6460472-6460491 None:intergenic 30.0%
! TCGTCAAAACCATATTGATT+TGG - chr4.3:6459743-6459762 MS.gene048767:intron 30.0%
! TCTTACAAGGTAACTTTTAC+GGG - chr4.3:6459908-6459927 MS.gene048767:intron 30.0%
! TTTTGTCTATAGGCAAAGTA+TGG - chr4.3:6460000-6460019 MS.gene048767:CDS 30.0%
!!! ACCATATTGATTTGGTGTTT+TGG - chr4.3:6459751-6459770 MS.gene048767:CDS 30.0%
!!! CAAGATATTTTGGAGTTTGA+TGG - chr4.3:6459621-6459640 MS.gene048767:intron 30.0%
!!! TAGTTGGCTTTTCAATGAAT+AGG - chr4.3:6460756-6460775 MS.gene048767:intron 30.0%
AACCCAAAAGTAGAATACGA+CGG - chr4.3:6459875-6459894 MS.gene048767:intron 35.0%
AAGCATGCTATGTTGATATG+AGG - chr4.3:6459819-6459838 MS.gene048767:CDS 35.0%
AAGTTCTCAATATGTTAGGG+TGG + chr4.3:6459348-6459367 None:intergenic 35.0%
ACATGATACCGAGACATTAA+GGG - chr4.3:6460609-6460628 MS.gene048767:intron 35.0%
ACATTTCTTACAGCACCTTA+AGG + chr4.3:6460490-6460509 None:intergenic 35.0%
AGCATGCTATGTTGATATGA+GGG - chr4.3:6459820-6459839 MS.gene048767:CDS 35.0%
AGCTTCTTCAACTTATACGT+AGG - chr4.3:6460040-6460059 MS.gene048767:CDS 35.0%
AGGTAGAATGACTTCTGTTA+GGG - chr4.3:6460127-6460146 MS.gene048767:intron 35.0%
AGTTCTCAATATGTTAGGGT+GGG + chr4.3:6459347-6459366 None:intergenic 35.0%
ATCACTTATGTAGAATGGTG+TGG - chr4.3:6459496-6459515 MS.gene048767:intron 35.0%
ATCCACCTTAATGTTTGCTT+TGG + chr4.3:6460883-6460902 None:intergenic 35.0%
ATGCATCTTGTAGATCGTTA+TGG + chr4.3:6459574-6459593 None:intergenic 35.0%
ATTCTTCTTAGACTTGGACT+TGG + chr4.3:6461060-6461079 None:intergenic 35.0%
CAAATTAAGCATACAAGGTC+AGG - chr4.3:6459402-6459421 MS.gene048767:intron 35.0%
CATGCTTCATTTATTTGCTC+TGG + chr4.3:6459806-6459825 None:intergenic 35.0%
CATGTAAACGTCAATGAGAA+TGG + chr4.3:6460594-6460613 None:intergenic 35.0%
GAATAGGAAGTGAATGAACA+AGG + chr4.3:6460532-6460551 None:intergenic 35.0%
GACGAATCAATTCCTAATTG+TGG + chr4.3:6459728-6459747 None:intergenic 35.0%
GAGAAGGATCTTCAAAAGAA+TGG - chr4.3:6460960-6460979 MS.gene048767:CDS 35.0%
GTAAGCTATCTTAAGTAGCA+TGG + chr4.3:6459695-6459714 None:intergenic 35.0%
TAACGATCTACAAGATGCAT+AGG - chr4.3:6459574-6459593 MS.gene048767:intron 35.0%
TACATGATACCGAGACATTA+AGG - chr4.3:6460608-6460627 MS.gene048767:intron 35.0%
TAGCAGAAAACCTAATACTC+CGG - chr4.3:6458819-6458838 MS.gene048767:CDS 35.0%
TCCACCTTAATGTTTGCTTT+GGG + chr4.3:6460882-6460901 None:intergenic 35.0%
! ATAGGCAAAGTATGGTCTTA+AGG - chr4.3:6460008-6460027 MS.gene048767:CDS 35.0%
! CATTTATTGGAAGTTTCTGG+TGG - chr4.3:6459038-6459057 MS.gene048767:CDS 35.0%
! GAGTTTGATGGATGTTATGT+TGG - chr4.3:6459633-6459652 MS.gene048767:intron 35.0%
!! AAGATGTTTTTGAGAGTCTG+AGG - chr4.3:6459849-6459868 MS.gene048767:intron 35.0%
!! TTAAGGTGCTGTAAGAAATG+TGG - chr4.3:6460489-6460508 MS.gene048767:intron 35.0%
!! TTTAGGTTCTGAAAAAGCTC+CGG + chr4.3:6461122-6461141 None:intergenic 35.0%
AAGCCCATCTGTTAAGTTTG+AGG - chr4.3:6459149-6459168 MS.gene048767:intron 40.0%
AAGTGAATGAACAAGGTAGC+AGG + chr4.3:6460525-6460544 None:intergenic 40.0%
AATGAACAAGGTAGCAGGAA+AGG + chr4.3:6460520-6460539 None:intergenic 40.0%
AATTCTCAAGTGACACTGAG+AGG - chr4.3:6458893-6458912 MS.gene048767:CDS 40.0%
ACCCAAAAGTAGAATACGAC+GGG - chr4.3:6459876-6459895 MS.gene048767:intron 40.0%
ACTTATACGTAGGTATCCAG+AGG - chr4.3:6460050-6460069 MS.gene048767:CDS 40.0%
AGGTCATATGCCTTAGTGAT+AGG - chr4.3:6460694-6460713 MS.gene048767:intron 40.0%
AGTTTGGCTAAATCACTGTG+GGG - chr4.3:6459243-6459262 MS.gene048767:intron 40.0%
ATGACCTAAAGCAGCTGTTT+CGG - chr4.3:6460904-6460923 MS.gene048767:CDS 40.0%
CAGGTAGAATGACTTCTGTT+AGG - chr4.3:6460126-6460145 MS.gene048767:intron 40.0%
CCACCTTAATGTTTGCTTTG+GGG + chr4.3:6460881-6460900 None:intergenic 40.0%
CCTTTAGGTCAAAAACAGCA+AGG + chr4.3:6460077-6460096 None:intergenic 40.0%
CTTATACGTAGGTATCCAGA+GGG - chr4.3:6460051-6460070 MS.gene048767:CDS 40.0%
GAAAAAACGTCGTCAGGAAA+TGG + chr4.3:6460174-6460193 None:intergenic 40.0%
GAGTTTGGCTAAATCACTGT+GGG - chr4.3:6459242-6459261 MS.gene048767:intron 40.0%
GTGATATGTAAACGAACCTG+TGG + chr4.3:6459481-6459500 None:intergenic 40.0%
TCACTCTTCCCTTAATGTCT+CGG + chr4.3:6460620-6460639 None:intergenic 40.0%
TGACCCCAAAGCAAACATTA+AGG - chr4.3:6460875-6460894 MS.gene048767:intron 40.0%
TGAGAATGGAAGCAAAGAGA+AGG + chr4.3:6460580-6460599 None:intergenic 40.0%
TGAGTTTGGCTAAATCACTG+TGG - chr4.3:6459241-6459260 MS.gene048767:intron 40.0%
TGCAAGTCTTCCATCACATT+GGG + chr4.3:6460108-6460127 None:intergenic 40.0%
! TTAACAGATGGGCTTCTCAA+AGG + chr4.3:6459144-6459163 None:intergenic 40.0%
!! ATGTTTGCTTTGGGGTCATT+CGG + chr4.3:6460873-6460892 None:intergenic 40.0%
!!! CCTTGCTGTTTTTGACCTAA+AGG - chr4.3:6460074-6460093 MS.gene048767:intron 40.0%
AAAACCTAATACTCCGGTGG+TGG - chr4.3:6458825-6458844 MS.gene048767:CDS 45.0%
AAGATGGGAATTGGTCAGCT+TGG + chr4.3:6461099-6461118 None:intergenic 45.0%
AAGTAGAATACGACGGGCAA+CGG - chr4.3:6459882-6459901 MS.gene048767:intron 45.0%
AATATACGTAAAGCCCCCGT+TGG - chr4.3:6458931-6458950 MS.gene048767:CDS 45.0%
AGGACTGCAGCACAAATTGA+TGG - chr4.3:6461014-6461033 MS.gene048767:CDS 45.0%
CAGAAAACCTAATACTCCGG+TGG - chr4.3:6458822-6458841 MS.gene048767:CDS 45.0%
CATTGGGGTAAGCATCCTTT+AGG + chr4.3:6460092-6460111 None:intergenic 45.0%
CCCCAAAGCAAACATTAAGG+TGG - chr4.3:6460878-6460897 MS.gene048767:intron 45.0%
CTCTGCAAGAGCAACTAGAT+AGG - chr4.3:6458734-6458753 MS.gene048767:CDS 45.0%
CTGAAAAAGCTCCGGAAGAT+GGG + chr4.3:6461114-6461133 None:intergenic 45.0%
CTGCAAGTCTTCCATCACAT+TGG + chr4.3:6460109-6460128 None:intergenic 45.0%
CTTAGACTTGGACTTGGACT+TGG + chr4.3:6461054-6461073 None:intergenic 45.0%
GCAAGTCTTCCATCACATTG+GGG + chr4.3:6460107-6460126 None:intergenic 45.0%
GCTACTGAAAAAACGTCGTC+AGG + chr4.3:6460180-6460199 None:intergenic 45.0%
GCTGCAAATGCATCAGCAAA+TGG - chr4.3:6458847-6458866 MS.gene048767:CDS 45.0%
GCTGTCCAACTTTGATTCAC+AGG - chr4.3:6460833-6460852 MS.gene048767:intron 45.0%
GTCGTCCTGTGAATCAAAGT+TGG + chr4.3:6460841-6460860 None:intergenic 45.0%
TATGTAAACGAACCTGTGGC+AGG + chr4.3:6459477-6459496 None:intergenic 45.0%
TCTCAATATGTTAGGGTGGG+CGG + chr4.3:6459344-6459363 None:intergenic 45.0%
TCTGAAAAAGCTCCGGAAGA+TGG + chr4.3:6461115-6461134 None:intergenic 45.0%
! ATTGGTCAGCTTGGTTCTCT+TGG + chr4.3:6461090-6461109 None:intergenic 45.0%
! CAATGTGATGGAAGACTTGC+AGG - chr4.3:6460107-6460126 MS.gene048767:intron 45.0%
! TACTCCGAAACAGCTGCTTT+AGG + chr4.3:6460911-6460930 None:intergenic 45.0%
! TGCCCGTCGTATTCTACTTT+TGG + chr4.3:6459881-6459900 None:intergenic 45.0%
! TTTTCCCTCGTCTCATGCAT+CGG + chr4.3:6459080-6459099 None:intergenic 45.0%
!! ACGGTTTTAATGCCAAGGGT+TGG + chr4.3:6460393-6460412 None:intergenic 45.0%
!! CGGTTTTAATGCCAAGGGTT+GGG + chr4.3:6460392-6460411 None:intergenic 45.0%
!! GCCCGTCGTATTCTACTTTT+GGG + chr4.3:6459880-6459899 None:intergenic 45.0%
!! GGCAACGGTTTTAATGCCAA+GGG + chr4.3:6460397-6460416 None:intergenic 45.0%
!! TGGCAACGGTTTTAATGCCA+AGG + chr4.3:6460398-6460417 None:intergenic 45.0%
!! TGTAGGCTTTGAAAGCTGCA+GGG - chr4.3:6460799-6460818 MS.gene048767:intron 45.0%
!! TGTTTGCTTTGGGGTCATTC+GGG + chr4.3:6460872-6460891 None:intergenic 45.0%
!! TTGTAGGCTTTGAAAGCTGC+AGG - chr4.3:6460798-6460817 MS.gene048767:intron 45.0%
!!! CGACGTTTTTTCAGTAGCAG+TGG - chr4.3:6460182-6460201 MS.gene048767:intron 45.0%
!!! TTCCACTTTTTCCCAACCCT+TGG - chr4.3:6460378-6460397 MS.gene048767:intron 45.0%
AAACGAACCTGTGGCAGGAT+TGG + chr4.3:6459472-6459491 None:intergenic 50.0%
AACGAACCTGTGGCAGGATT+GGG + chr4.3:6459471-6459490 None:intergenic 50.0%
AAGCTCCGGAAGATGGGAAT+TGG + chr4.3:6461108-6461127 None:intergenic 50.0%
ACGCTTCATCTGAGCACCAA+CGG + chr4.3:6458950-6458969 None:intergenic 50.0%
AGCATTGCGTCGAGCAAAGT+TGG - chr4.3:6458796-6458815 MS.gene048767:CDS 50.0%
AGTCCTCCTCTTTGCAGTGT+TGG + chr4.3:6461000-6461019 None:intergenic 50.0%
CTACCAACACTGCAAAGAGG+AGG - chr4.3:6460994-6461013 MS.gene048767:CDS 50.0%
CTGCTACCAACACTGCAAAG+AGG - chr4.3:6460991-6461010 MS.gene048767:CDS 50.0%
CTTGGACTTGGACTTGGACT+TGG + chr4.3:6461048-6461067 None:intergenic 50.0%
GATTCCGATGCATGAGACGA+GGG - chr4.3:6459073-6459092 MS.gene048767:intron 50.0%
GCTGACCAATTCCCATCTTC+CGG - chr4.3:6461100-6461119 MS.gene048767:CDS 50.0%
GGATGCTTACCCCAATGTGA+TGG - chr4.3:6460095-6460114 MS.gene048767:intron 50.0%
GTTTGGCTGGACACTGAGTT+TGG - chr4.3:6459227-6459246 MS.gene048767:intron 50.0%
TCAAAAACAGCAAGGCCCTC+TGG + chr4.3:6460069-6460088 None:intergenic 50.0%
TCAAATCCCAATCCTGCCAC+AGG - chr4.3:6459462-6459481 MS.gene048767:intron 50.0%
TGAAAGCTGCAGGGCAGATT+TGG - chr4.3:6460808-6460827 MS.gene048767:intron 50.0%
TGATTCCGATGCATGAGACG+AGG - chr4.3:6459072-6459091 MS.gene048767:intron 50.0%
TGCCAAGGGTTGGGAAAAAG+TGG + chr4.3:6460383-6460402 None:intergenic 50.0%
!! CTCTTTGCAGTGTTGGTAGC+AGG + chr4.3:6460993-6461012 None:intergenic 50.0%
CGCTTCATCTGAGCACCAAC+GGG + chr4.3:6458949-6458968 None:intergenic 55.0%
CGGGCAACGGTTCTCTTACA+AGG - chr4.3:6459895-6459914 MS.gene048767:intron 55.0%
CTTCATCTGAGCACCAACGG+GGG + chr4.3:6458947-6458966 None:intergenic 55.0%
GATGCATTTGCAGCCACCAC+CGG + chr4.3:6458841-6458860 None:intergenic 55.0%
GCTTCATCTGAGCACCAACG+GGG + chr4.3:6458948-6458967 None:intergenic 55.0%
! GCAGCCACCACCGGAGTATT+AGG + chr4.3:6458832-6458851 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 6458730 6461145 6458730 ID=MS.gene048767
chr4.3 mRNA 6458730 6461145 6458730 ID=MS.gene048767.t1;Parent=MS.gene048767
chr4.3 exon 6460885 6461145 6460885 ID=MS.gene048767.t1.exon1;Parent=MS.gene048767.t1
chr4.3 CDS 6460885 6461145 6460885 ID=cds.MS.gene048767.t1;Parent=MS.gene048767.t1
chr4.3 exon 6459959 6460090 6459959 ID=MS.gene048767.t1.exon2;Parent=MS.gene048767.t1
chr4.3 CDS 6459959 6460090 6459959 ID=cds.MS.gene048767.t1;Parent=MS.gene048767.t1
chr4.3 exon 6459747 6459863 6459747 ID=MS.gene048767.t1.exon3;Parent=MS.gene048767.t1
chr4.3 CDS 6459747 6459863 6459747 ID=cds.MS.gene048767.t1;Parent=MS.gene048767.t1
chr4.3 exon 6458730 6459071 6458730 ID=MS.gene048767.t1.exon4;Parent=MS.gene048767.t1
chr4.3 CDS 6458730 6459071 6458730 ID=cds.MS.gene048767.t1;Parent=MS.gene048767.t1
Gene Sequence

>MS.gene048767

ATGGCTCTGCAAGAGCAACTAGATAGGTTCAAGAAACAACAAGAGAAGTGTCAATCGACACTCTCGAGCATTGCGTCGAGCAAAGTTGGTAGCAGAAAACCTAATACTCCGGTGGTGGCTGCAAATGCATCAGCAAATGGAAGAAATTCAAAAACTGGTGTTAAATTCTCAAGTGACACTGAGAGGCTTCAGCAGATTAATAATATACGTAAAGCCCCCGTTGGTGCTCAGATGAAGCGTGTTATTGATCTATTGCTTGAGGTAATTTTTGGTTTTCTCATTCAATTCAATTTTGTTGTTTGTTTTTTCATTTATTGGAAGTTTCTGGTGGTGTTATTGCAGTGATTCCGATGCATGAGACGAGGGAAAAAAAATGAATTTTATTAGAATATATACACTCATTTTACATATCCTTTGAGAAGCCCATCTGTTAAGTTTGAGGTTAATTGAGACACTATAGCTCAAAGTCACCAATTGCAAATTGAAACTAATAATATGTTTGGCTGGACACTGAGTTTGGCTAAATCACTGTGGGGCTGTGATTTTGTTGAAGCTTCAACGTGTAGCTTTTGCTGAAATCGCTGTTACGCACTGTGATTTCGTCAAACTCACCGCCCACCCTAACATATTGAGAACTTAATTTAAGAAGCAAAAACGTTTTTTTATTGTTTGCAAATTAAGCATACAAGGTCAGGTTATGACTTAAATAGATGACATAAAGGGTCAATTTGTTCAAATCCCAATCCTGCCACAGGTTCGTTTACATATCACTTATGTAGAATGGTGTGGTACGTAGTTTGAAAATTGTATTCCGTGAACATTGATTAAGAGTATTTTTTTTCCATAACGATCTACAAGATGCATAGGTATGCTTTACTTGCAGTCATCTTCCAAGATATTTTGGAGTTTGATGGATGTTATGTTGGTTCTCTTAAACTGAGTTGAATTCTTATTTTGAAGAGCCATGCTACTTAAGATAGCTTACCAATGAAATTCCACAATTAGGAATTGATTCGTCAAAACCATATTGATTTGGTGTTTTGGTTTGCTGATAGACACGACAAGCTTTTACACCAGAGCAAATAAATGAAGCATGCTATGTTGATATGAGGGCTAACAAAGATGTTTTTGAGAGTCTGAGGAAAAACCCAAAAGTAGAATACGACGGGCAACGGTTCTCTTACAAGGTAACTTTTACGGGTTCATATATCTTAATAGTTTTATATTTAAATACTCCTTTAGCAAATAACTTCTTCATCTGTTATTTGTTTTTTGTCTATAGGCAAAGTATGGTCTTAAGGAGAAAAGCGAGCTTCTTCAACTTATACGTAGGTATCCAGAGGGCCTTGCTGTTTTTGACCTAAAGGATGCTTACCCCAATGTGATGGAAGACTTGCAGGTAGAATGACTTCTGTTAGGGCTGTTTTCTTTTCTTTGAAGACCATTTCCTGACGACGTTTTTTCAGTAGCAGTGGCTGTTTTGTTCATAGATATATGTTAATTCTCATTTCTCACAGATTGGACTTTTGTCTAGATAATGTGTTTTGTAAGAAATATTAACTGAAACACTATACCCTTTTGAAGAAGATCAAATGTTTTATGGGTAATATAATAACATTGCACATTAGTGGGTTTTATAAAATCGTTTTTCCACTTTTTCCCAACCCTTGGCATTAAAACCGTTGCCATTATTATAAATTAAGGTTAATACTGAGGAAATTGAAACATCTTATAATTAGCCATTACAAAATCTTGCTCCTTAAGGTGCTGTAAGAAATGTGGTTCAACCTTTCCTGCTACCTTGTTCATTCACTTCCTATTCATTTGTTTTGTTTATTTTATAAAGACCTTCTCTTTGCTTCCATTCTCATTGACGTTTACATGATACCGAGACATTAAGGGAAGAGTGATTTTTTTAATGTTTGTCTTGAAATATGGAAATAGAGATTATAGATGGAACTATCAGGTCATATGCCTTAGTGATAGGTGATGTTTTTGTAGAAGTGAAGAGTCAAAATCTTGTATATAGTTGGCTTTTCAATGAATAGGATCTCATAATGATATCTTTTTGTAGGCTTTGAAAGCTGCAGGGCAGATTTGGCTGCTGTCCAACTTTGATTCACAGGACGACATTGCATACCCGAATGACCCCAAAGCAAACATTAAGGTGGATGATGACCTAAAGCAGCTGTTTCGGAGTATTGAGTTACCTCGTGATATGATTGATATAGAGAAGGATCTTCAAAAGAATGGAATGAAGCCTGCTACCAACACTGCAAAGAGGAGGACTGCAGCACAAATTGATGGCGTTTCTTCCAAGTCCAAGTCCAAGTCCAAGTCTAAGAAGAATGAAATCACCAAGAGAACCAAGCTGACCAATTCCCATCTTCCGGAGCTTTTTCAGAACCTAAAGTAA
Protein sequence

>MS.gene048767.t1

MALQEQLDRFKKQQEKCQSTLSSIASSKVGSRKPNTPVVAANASANGRNSKTGVKFSSDTERLQQINNIRKAPVGAQMKRVIDLLLETRQAFTPEQINEACYVDMRANKDVFESLRKNPKVEYDGQRFSYKAKYGLKEKSELLQLIRRYPEGLAVFDLKDAYPNVMEDLQALKAAGQIWLLSNFDSQDDIAYPNDPKANIKVDDDLKQLFRSIELPRDMIDIEKDLQKNGMKPATNTAKRRTAAQIDGVSSKSKSKSKSKKNEITKRTKLTNSHLPELFQNLK