AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene048977

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048977.t1 MTR_8g100075 99.046 629 6 0 1 629 1 629 0.0 1305
MS.gene048977.t1 MTR_0019s0120 56.390 626 262 5 9 628 14 634 0.0 773
MS.gene048977.t1 MTR_7g076170 60.032 618 224 6 20 622 18 627 0.0 771
MS.gene048977.t1 MTR_8g432680 57.800 609 249 5 22 622 24 632 0.0 751
MS.gene048977.t1 MTR_1g101370 57.167 600 243 6 28 621 27 618 0.0 746
MS.gene048977.t1 MTR_4g098890 56.551 603 232 7 25 617 18 600 0.0 729
MS.gene048977.t1 MTR_8g055840 54.023 609 258 5 25 628 17 608 0.0 710
MS.gene048977.t1 MTR_5g040360 52.979 621 263 7 17 629 9 608 0.0 693
MS.gene048977.t1 MTR_3g040370 52.451 612 258 6 25 628 19 605 0.0 666
MS.gene048977.t1 MTR_0019s0120 56.084 526 220 5 9 528 14 534 0.0 644
MS.gene048977.t1 MTR_7g006060 49.815 540 265 3 81 614 26 565 0.0 601
MS.gene048977.t1 MTR_8g071280 44.677 573 303 10 61 627 31 595 1.93e-175 511
MS.gene048977.t1 MTR_5g026930 46.768 526 270 7 98 620 72 590 5.50e-173 505
MS.gene048977.t1 MTR_7g076150 43.805 573 282 10 63 620 40 587 3.36e-172 503
MS.gene048977.t1 MTR_8g432620 42.328 567 297 8 63 621 57 601 8.12e-170 497
MS.gene048977.t1 MTR_1g442840 45.825 515 249 11 96 596 90 588 1.99e-155 461
MS.gene048977.t1 MTR_5g080720 42.222 540 271 11 95 617 122 637 3.09e-154 459
MS.gene048977.t1 MTR_1g442840 46.516 488 235 10 119 596 4 475 1.63e-151 447
MS.gene048977.t1 MTR_1g069785 41.681 571 293 14 73 620 32 585 1.09e-149 446
MS.gene048977.t1 MTR_1g075900 42.572 552 280 14 96 627 89 623 2.40e-147 441
MS.gene048977.t1 MTR_3g068990 41.744 539 289 10 88 617 143 665 4.61e-147 441
MS.gene048977.t1 MTR_7g076150 45.923 466 218 8 167 620 1 444 8.29e-147 432
MS.gene048977.t1 MTR_1g029730 40.602 532 283 11 97 619 151 658 1.33e-142 429
MS.gene048977.t1 MTR_5g026930 46.774 434 222 6 98 528 72 499 1.54e-141 421
MS.gene048977.t1 MTR_3g096480 40.489 573 302 16 63 620 228 776 8.80e-138 421
MS.gene048977.t1 MTR_5g080720 42.500 480 235 11 95 557 122 577 2.32e-134 405
MS.gene048977.t1 MTR_3g088790 41.045 536 281 15 101 620 226 742 2.52e-133 409
MS.gene048977.t1 MTR_8g093880 40.182 550 294 16 63 596 256 786 1.45e-132 409
MS.gene048977.t1 MTR_5g083150 39.810 525 278 15 107 620 101 598 5.36e-130 395
MS.gene048977.t1 MTR_1g051055 36.305 617 342 12 25 621 19 604 5.82e-130 395
MS.gene048977.t1 MTR_5g021100 38.951 534 290 14 101 620 322 833 3.67e-129 400
MS.gene048977.t1 MTR_3g071530 38.388 521 287 8 107 620 153 646 2.36e-128 392
MS.gene048977.t1 MTR_8g076650 38.752 529 297 11 101 620 380 890 2.39e-124 389
MS.gene048977.t1 MTR_1g022255 34.981 526 293 15 108 620 210 699 2.26e-97 313
MS.gene048977.t1 MTR_4g083030 32.613 555 328 15 87 619 73 603 6.11e-96 307
MS.gene048977.t1 MTR_3g074780 32.155 566 316 16 95 620 151 688 2.02e-94 305
MS.gene048977.t1 MTR_5g064080 31.429 560 324 16 96 624 144 674 6.15e-92 298
MS.gene048977.t1 MTR_1g022255 29.517 393 234 12 108 488 210 571 2.45e-50 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048977.t1 AT1G33170 71.520 625 160 4 16 622 12 636 0.0 959
MS.gene048977.t1 AT4G10440 70.906 629 173 5 1 626 1 622 0.0 939
MS.gene048977.t1 AT2G45750 58.576 618 241 6 17 626 11 621 0.0 761
MS.gene048977.t1 AT4G00750 55.217 623 265 5 15 629 15 631 0.0 738
MS.gene048977.t1 AT1G26850 55.354 607 246 5 25 621 18 609 0.0 712
MS.gene048977.t1 AT1G26850 55.354 607 246 5 25 621 18 609 0.0 712
MS.gene048977.t1 AT4G18030 52.674 617 272 8 12 623 5 606 0.0 677
MS.gene048977.t1 AT2G43200 50.860 523 251 4 98 618 91 609 0.0 595
MS.gene048977.t1 AT2G43200 50.860 523 251 4 98 618 91 609 0.0 595
MS.gene048977.t1 AT1G26850 54.600 500 202 5 25 514 18 502 0.0 565
MS.gene048977.t1 AT1G31850 47.917 528 263 8 98 620 76 596 0.0 539
MS.gene048977.t1 AT1G31850 47.917 528 263 8 98 620 76 596 0.0 539
MS.gene048977.t1 AT1G31850 47.917 528 263 8 98 620 76 596 0.0 539
MS.gene048977.t1 AT1G31850 47.917 528 263 8 98 620 76 596 0.0 539
MS.gene048977.t1 AT4G19120 46.715 548 274 9 82 620 52 590 0.0 530
MS.gene048977.t1 AT4G19120 46.715 548 274 9 82 620 52 590 0.0 530
MS.gene048977.t1 AT4G00740 43.182 528 274 7 101 620 84 593 1.18e-158 469
MS.gene048977.t1 AT4G00740 43.182 528 274 7 101 620 84 593 1.18e-158 469
MS.gene048977.t1 AT5G06050 40.422 569 295 12 71 619 119 663 2.57e-154 460
MS.gene048977.t1 AT4G19120 46.930 456 225 8 82 528 52 499 1.70e-150 444
MS.gene048977.t1 AT2G39750 39.831 590 306 13 52 617 125 689 1.64e-149 448
MS.gene048977.t1 AT1G77260 41.917 532 282 10 95 617 140 653 1.27e-147 442
MS.gene048977.t1 AT1G04430 42.336 548 277 12 96 620 82 613 3.91e-146 437
MS.gene048977.t1 AT1G04430 42.336 548 277 12 96 620 82 613 3.91e-146 437
MS.gene048977.t1 AT1G04430 42.336 548 277 12 96 620 82 613 3.91e-146 437
MS.gene048977.t1 AT4G14360 43.883 515 259 12 96 596 74 572 1.02e-145 436
MS.gene048977.t1 AT4G14360 43.883 515 259 12 96 596 74 572 1.02e-145 436
MS.gene048977.t1 AT3G23300 42.144 541 281 13 96 620 77 601 4.95e-144 431
MS.gene048977.t1 AT3G23300 42.144 541 281 13 96 620 77 601 4.95e-144 431
MS.gene048977.t1 AT5G14430 42.647 544 278 14 96 621 78 605 1.94e-139 420
MS.gene048977.t1 AT5G14430 42.279 544 280 14 96 621 78 605 1.84e-138 417
MS.gene048977.t1 AT2G34300 40.208 577 307 16 58 620 205 757 3.86e-136 416
MS.gene048977.t1 AT2G34300 40.208 577 307 16 58 620 205 757 3.86e-136 416
MS.gene048977.t1 AT2G34300 40.208 577 307 16 58 620 205 757 3.86e-136 416
MS.gene048977.t1 AT1G29470 42.135 534 276 15 101 620 243 757 2.16e-135 414
MS.gene048977.t1 AT1G29470 42.135 534 276 15 101 620 243 757 2.16e-135 414
MS.gene048977.t1 AT2G40280 40.571 525 268 13 107 620 90 581 1.03e-133 404
MS.gene048977.t1 AT5G64030 39.062 576 308 16 63 620 266 816 9.42e-132 407
MS.gene048977.t1 AT3G51070 40.706 538 279 16 101 620 374 889 4.43e-130 404
MS.gene048977.t1 AT3G56080 39.734 526 274 15 107 620 114 608 3.87e-129 393
MS.gene048977.t1 AT3G10200 37.313 536 300 9 101 621 75 589 9.10e-129 392
MS.gene048977.t1 AT5G04060 36.517 534 305 8 101 621 86 598 7.21e-127 387
MS.gene048977.t1 AT3G10200 39.175 485 260 8 101 571 75 538 1.04e-122 376
MS.gene048977.t1 AT3G51070 42.766 470 237 14 101 554 374 827 3.32e-116 366
MS.gene048977.t1 AT3G56080 41.087 460 236 14 107 554 114 550 1.20e-114 354
MS.gene048977.t1 AT1G19430 35.038 528 291 17 108 620 232 722 2.81e-95 308
MS.gene048977.t1 AT1G19430 35.038 528 291 17 108 620 232 722 2.81e-95 308
MS.gene048977.t1 AT1G13860 32.613 555 308 15 95 617 78 598 1.81e-93 300
MS.gene048977.t1 AT1G13860 32.613 555 308 15 95 617 78 598 1.81e-93 300
MS.gene048977.t1 AT1G13860 32.613 555 308 15 95 617 78 598 1.81e-93 300
MS.gene048977.t1 AT1G13860 32.613 555 308 15 95 617 78 598 1.81e-93 300
MS.gene048977.t1 AT2G03480 31.119 572 319 14 77 619 67 592 2.38e-90 292
MS.gene048977.t1 AT2G03480 31.607 560 343 13 77 619 67 603 7.74e-89 288
MS.gene048977.t1 AT1G78240 31.004 558 328 17 96 619 144 678 2.16e-87 286
MS.gene048977.t1 AT1G78240 31.004 558 328 17 96 619 144 678 2.16e-87 286
MS.gene048977.t1 AT1G13860 33.693 463 255 13 184 617 3 442 9.83e-82 265
MS.gene048977.t1 AT1G19430 31.313 396 226 14 108 489 232 595 1.15e-53 194
MS.gene048977.t1 AT2G03480 30.792 341 217 6 77 408 67 397 9.46e-52 184

Find 139 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTTATTGGATTCTTTC+TGG 0.100373 4.1:-4044432 MS.gene048977:CDS
TTAAAAGGTGTCTTATATTT+TGG 0.133343 4.1:+4045245 None:intergenic
TTGAGAAAGCTGTTCAAAAT+TGG 0.200150 4.1:-4045163 MS.gene048977:CDS
ATTCATGCAGCTGGTGTATT+TGG 0.210043 4.1:-4043598 MS.gene048977:CDS
TCCTTAGGCCAGGTGGTTAT+TGG 0.243011 4.1:-4044443 MS.gene048977:CDS
CTACTTTGAGGCCATTTAAA+AGG 0.243262 4.1:+4045230 None:intergenic
CTCATTCCATGGAGCAAATA+TGG 0.258315 4.1:-4044669 MS.gene048977:intron
TTCAAAAGGACAATGAATTA+TGG 0.259353 4.1:-4043870 MS.gene048977:CDS
CTATATCTTCCTTGAGCAAT+AGG 0.274060 4.1:+4043814 None:intergenic
GCGTTTGGCAACTTCCTCTA+TGG 0.300697 4.1:+4044335 None:intergenic
CATTATTAAAATTTCCAGTT+TGG 0.305235 4.1:-4043747 MS.gene048977:CDS
ACAGAAACATGTTGAAATAT+AGG 0.306859 4.1:-4045322 MS.gene048977:CDS
CAACATTACTGTTATACTAT+TGG 0.313122 4.1:-4043363 MS.gene048977:CDS
TCACTTGAAATAAGTACTTC+TGG 0.335289 4.1:-4045428 MS.gene048977:CDS
GGAAGAAATACTGGAGGGGC+TGG 0.344229 4.1:-4044395 MS.gene048977:CDS
ATTGAACCACTACTAATTCT+AGG 0.344619 4.1:+4043916 None:intergenic
ATTATTAAAATTTCCAGTTT+GGG 0.346106 4.1:-4043746 MS.gene048977:CDS
CAGTATTTCTTCCATCTAAT+AGG 0.347198 4.1:+4044405 None:intergenic
AATTATAAAGGTAGCAAGTT+GGG 0.358008 4.1:-4044868 MS.gene048977:intron
CCATGTGAGTCTATTTCTCT+TGG 0.358721 4.1:+4045634 None:intergenic
ATCTTTGAAAGAGGTTTCAT+TGG 0.366006 4.1:-4043676 MS.gene048977:CDS
TTTCTGGCCCACCTATTAGA+TGG 0.370798 4.1:-4044416 MS.gene048977:CDS
TGGTGATCCATTGTAATCCT+TGG 0.371322 4.1:+4045679 None:intergenic
TCAAGCTGATTATAATGCTT+TGG 0.372036 4.1:+4045659 None:intergenic
CAGAAACATGTTGAAATATA+GGG 0.373112 4.1:-4045321 MS.gene048977:CDS
TTGCTTGAGCTGGTAACTTC+TGG 0.378404 4.1:+4043991 None:intergenic
GGTGATCCATTGTAATCCTT+GGG 0.378516 4.1:+4045680 None:intergenic
GCCATATCAAAAGCCCTTGC+TGG 0.383647 4.1:+4044708 None:intergenic
CTATTAGATGGAAGAAATAC+TGG 0.384247 4.1:-4044404 MS.gene048977:CDS
ATCAGAACTGCAATTGATAC+AGG 0.390635 4.1:-4045026 MS.gene048977:CDS
TGGTGTATTTGGCATATATC+AGG 0.393487 4.1:-4043587 MS.gene048977:CDS
TCCTGCTATGATTGGTATCA+TGG 0.402427 4.1:-4044751 MS.gene048977:CDS
ACAACATTCATAACCCAAAC+TGG 0.404193 4.1:+4043733 None:intergenic
TGAATGAGATCATAAGTTCT+AGG 0.405756 4.1:+4043616 None:intergenic
TCATTGGGACTTACCATGAT+TGG 0.417773 4.1:-4043660 MS.gene048977:CDS
TCCAGCTACTTTGCTTGAGC+TGG 0.418390 4.1:+4043981 None:intergenic
ATTGATATGAATGCTTATCT+TGG 0.425704 4.1:-4043784 MS.gene048977:CDS
TTTCTTCCAACATATGCGTT+TGG 0.430721 4.1:+4044320 None:intergenic
TATGCCTCAGATTGGTTAGT+AGG 0.432612 4.1:+4045545 None:intergenic
GTTTAGTTTGTACACATTCA+AGG 0.437945 4.1:+4044250 None:intergenic
GTTCTCGCTGCCTCATTCCA+TGG 0.445475 4.1:-4044680 MS.gene048977:CDS
CAAAATTGGATTCAAGTTGA+AGG 0.446164 4.1:-4045149 MS.gene048977:CDS
AGAAAGATGACCTTTCCATT+TGG 0.448933 4.1:-4044290 MS.gene048977:CDS
TCAAAAGTAGATTGTAATGT+TGG 0.460424 4.1:-4045521 MS.gene048977:CDS
AATTCAGCTCATGATACTCT+TGG 0.465587 4.1:-4043703 MS.gene048977:CDS
CAAGCATAATCTCTACTTTG+AGG 0.466308 4.1:+4045218 None:intergenic
GAGCTTATTCCTCTTACTAC+TGG 0.466754 4.1:-4045053 MS.gene048977:CDS
CCCAGAAAAGATTCTTGTTG+CGG 0.467901 4.1:-4043204 MS.gene048977:CDS
TCTTTGAAAGAGGTTTCATT+GGG 0.470943 4.1:-4043675 MS.gene048977:CDS
CTGATATTACCAGTAGTAAG+AGG 0.472181 4.1:+4045044 None:intergenic
GGTGATCGGTTTCGATTCCC+TGG 0.472490 4.1:-4045128 MS.gene048977:CDS
GTATCATGAGCTGAATTTGA+AGG 0.474584 4.1:+4043709 None:intergenic
GAAGTGGAAGAGCAACATAA+TGG 0.475548 4.1:-4043249 MS.gene048977:CDS
TCCAATAACCACCTGGCCTA+AGG 0.482017 4.1:+4044442 None:intergenic
CTTGGTGCAATCTTTGAAAG+AGG 0.483757 4.1:-4043685 MS.gene048977:CDS
AGAAAGCTTCACACCAATCA+TGG 0.487974 4.1:+4043647 None:intergenic
TCAAAAGCCCTTGCTGGGTA+TGG 0.491448 4.1:+4044714 None:intergenic
ACCAGCTCAAGCAAAGTAGC+TGG 0.491717 4.1:-4043982 MS.gene048977:CDS
AATACTTACCAAGCCATGTC+AGG 0.492107 4.1:+4044189 None:intergenic
AGAGGAGTTCCTGCTATGAT+TGG 0.493820 4.1:-4044759 MS.gene048977:CDS
GATATGAATGCTTATCTTGG+TGG 0.494234 4.1:-4043781 MS.gene048977:CDS
CTGATAATCCTGACATGGCT+TGG 0.495727 4.1:-4044197 MS.gene048977:intron
TACTGTTATACTATTGGAGA+TGG 0.501641 4.1:-4043357 MS.gene048977:CDS
GGATTCAAGTTGAAGGTGAT+CGG 0.505103 4.1:-4045142 MS.gene048977:CDS
GAAGAAATACTGGAGGGGCT+GGG 0.506084 4.1:-4044394 MS.gene048977:CDS
TTTCCATGCACCCATTACAT+AGG 0.506215 4.1:+4045580 None:intergenic
AGCTCAGGTACAGTTTGCAT+TGG 0.506264 4.1:-4044784 MS.gene048977:CDS
TAATTATAAAGGTAGCAAGT+TGG 0.506609 4.1:-4044869 MS.gene048977:intron
CGGTTTCGATTCCCTGGTGG+AGG 0.508041 4.1:-4045122 MS.gene048977:CDS
TGAGCTTCATGTGTATCTCT+TGG 0.511536 4.1:+4044801 None:intergenic
AATTTCTCTGCAGTGATGCT+TGG 0.514766 4.1:+4043892 None:intergenic
TATGATCTCATTCATGCAGC+TGG 0.517745 4.1:-4043607 MS.gene048977:CDS
GAGCTGGTAACTTCTGGTAA+TGG 0.518242 4.1:+4043997 None:intergenic
CTGAATACAACTGTACCTTC+TGG 0.521917 4.1:+4043320 None:intergenic
CTCAAAGTAGAGATTATGCT+TGG 0.522721 4.1:-4045217 MS.gene048977:CDS
TGTAATGTTGGATCAACAGC+AGG 0.524580 4.1:-4045509 MS.gene048977:CDS
CGAGGACGATGATGAAATTG+AGG 0.528468 4.1:+4045481 None:intergenic
CACTGCAGAGAAATTTCAAA+AGG 0.531156 4.1:-4043884 MS.gene048977:CDS
TATTTCTTCCATCTAATAGG+TGG 0.536838 4.1:+4044408 None:intergenic
CCAGTCTGATAATCCTGACA+TGG 0.541896 4.1:-4044202 MS.gene048977:CDS
GGTACTGCAAAGGCACGTCT+TGG 0.542582 4.1:+4043940 None:intergenic
AAGAGATACACATGAAGCTC+AGG 0.543833 4.1:-4044799 MS.gene048977:CDS
CATATTTGCTCCATGGAATG+AGG 0.545022 4.1:+4044670 None:intergenic
GGAAACATGGTACCTCCACC+AGG 0.545167 4.1:+4045110 None:intergenic
CCATGTCAGGATTATCAGAC+TGG 0.545882 4.1:+4044202 None:intergenic
CCTCTAGCTGGATCTTGACA+TGG 0.546211 4.1:+4045356 None:intergenic
TTGAAGTGGATAGAGTCCTT+AGG 0.548105 4.1:-4044458 MS.gene048977:CDS
CCATATCAAAAGCCCTTGCT+GGG 0.549809 4.1:+4044709 None:intergenic
CATATCACTCCTATTGCTCA+AGG 0.550740 4.1:-4043823 MS.gene048977:CDS
GTGGATAGAGTCCTTAGGCC+AGG 0.552453 4.1:-4044453 MS.gene048977:CDS
CTCAAGCAAAGTAGCTGGAG+GGG 0.557038 4.1:-4043977 MS.gene048977:CDS
ATCAGCACCGCGAGGAAACA+TGG 0.563999 4.1:+4045097 None:intergenic
TACCAACCATATTTGCTCCA+TGG 0.566081 4.1:+4044663 None:intergenic
TGGTATGTATTTGATTGAAG+TGG 0.568021 4.1:-4044472 MS.gene048977:CDS
TATTTGGCATATATCAGGAT+AGG 0.572492 4.1:-4043582 MS.gene048977:intron
AACATAATGGATCATGAAAG+TGG 0.574348 4.1:-4043236 MS.gene048977:CDS
GAATGAGATCATAAGTTCTA+GGG 0.575775 4.1:+4043617 None:intergenic
GGTACAGTTGTATTCAGAGA+AGG 0.578876 4.1:-4043314 MS.gene048977:CDS
AAAGGCTACCATACCCAGCA+AGG 0.579111 4.1:-4044722 MS.gene048977:CDS
GCCATGATACCAATCATAGC+AGG 0.580506 4.1:+4044750 None:intergenic
ATTTCTTCCATCTAATAGGT+GGG 0.583039 4.1:+4044409 None:intergenic
GATAGAATCTTGAGGCCAGA+AGG 0.587910 4.1:-4043335 MS.gene048977:CDS
CCAAGAGAAATAGACTCACA+TGG 0.591381 4.1:-4045634 MS.gene048977:CDS
TTGAAATAAGTACTTCTGGT+GGG 0.591390 4.1:-4045424 MS.gene048977:CDS
ATTATAAAGGTAGCAAGTTG+GGG 0.592087 4.1:-4044867 MS.gene048977:intron
AAGTTGCCAAACGCATATGT+TGG 0.595240 4.1:-4044326 MS.gene048977:CDS
TTATCAGACTGGCAAATATG+TGG 0.598326 4.1:+4044213 None:intergenic
GTACAGTTTGCATTGGAGAG+AGG 0.599309 4.1:-4044777 MS.gene048977:CDS
AAAGGACAATGAATTATGGA+AGG 0.600355 4.1:-4043866 MS.gene048977:CDS
CTTGAAATAAGTACTTCTGG+TGG 0.600492 4.1:-4045425 MS.gene048977:CDS
GATCGGTTTCGATTCCCTGG+TGG 0.605900 4.1:-4045125 MS.gene048977:CDS
GCTCAAGCAAAGTAGCTGGA+GGG 0.607755 4.1:-4043978 MS.gene048977:CDS
AGCTCAAGCAAAGTAGCTGG+AGG 0.608310 4.1:-4043979 MS.gene048977:CDS
GCAAGAGCAAGATTCCATAG+AGG 0.609694 4.1:-4044349 MS.gene048977:CDS
TCAATGCTAAGCTCTTTGTG+TGG 0.613939 4.1:+4045182 None:intergenic
TGTCCTATGTAATGGGTGCA+TGG 0.614566 4.1:-4045583 MS.gene048977:CDS
GTGTTACTGATGCAATGAAG+TGG 0.625030 4.1:-4043265 MS.gene048977:CDS
GGGCTGGGAAAGAACTGAAG+AGG 0.627289 4.1:-4044379 MS.gene048977:CDS
TAGATGGAAGAAATACTGGA+GGG 0.629603 4.1:-4044400 MS.gene048977:CDS
TCACTATATGTCATGTCACA+TGG 0.634015 4.1:+4045386 None:intergenic
AGATGGAAGAAATACTGGAG+GGG 0.651671 4.1:-4044399 MS.gene048977:CDS
GAAAGAATCCAATAACCACC+TGG 0.654203 4.1:+4044435 None:intergenic
AAAATGCCCAAGGATTACAA+TGG 0.656503 4.1:-4045686 MS.gene048977:CDS
TCAGAACTGCAATTGATACA+GGG 0.660831 4.1:-4045025 MS.gene048977:CDS
AACACCTACTAACCAATCTG+AGG 0.662097 4.1:-4045549 MS.gene048977:CDS
TGGAGATGGATAGAATCTTG+AGG 0.664431 4.1:-4043343 MS.gene048977:CDS
CCATGTCAAGATCCAGCTAG+AGG 0.667824 4.1:-4045356 MS.gene048977:CDS
TTAGATGGAAGAAATACTGG+AGG 0.668874 4.1:-4044401 MS.gene048977:CDS
GTACCTCCTAGAATTAGTAG+TGG 0.671372 4.1:-4043922 MS.gene048977:CDS
AAGGCTACCATACCCAGCAA+GGG 0.674658 4.1:-4044721 MS.gene048977:CDS
ACTGCAATTGATACAGGGTG+TGG 0.682693 4.1:-4045020 MS.gene048977:CDS
AATTCTAGGAGGTACTGCAA+AGG 0.682768 4.1:+4043930 None:intergenic
GAACCACTACTAATTCTAGG+AGG 0.691410 4.1:+4043919 None:intergenic
CTATATGTCATGTCACATGG+TGG 0.691797 4.1:+4045389 None:intergenic
TTGGTATCATGGCTTCACAA+AGG 0.696029 4.1:-4044740 MS.gene048977:CDS
GATAGAGTCCTTAGGCCAGG+TGG 0.700562 4.1:-4044450 MS.gene048977:CDS
GGAGGTACCATGTTTCCTCG+CGG 0.714635 4.1:-4045104 MS.gene048977:CDS
GAAACATGGTACCTCCACCA+GGG 0.720834 4.1:+4045111 None:intergenic
ATGTACGCATCAGCACCGCG+AGG 0.786908 4.1:+4045089 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTAAAATTTCCAGTTT+GGG - chr4.1:4045097-4045116 MS.gene048977:CDS 15.0%
!!! ATTTAACCTTGTAATTTTAA+AGG + chr4.1:4044686-4044705 None:intergenic 15.0%
!!! TTTAACCTTGTAATTTTAAA+GGG + chr4.1:4044685-4044704 None:intergenic 15.0%
!! AATTAATTGTTATGTATGGA+AGG - chr4.1:4045455-4045474 MS.gene048977:CDS 20.0%
!! ACTGAATTAATTGTTATGTA+TGG - chr4.1:4045451-4045470 MS.gene048977:CDS 20.0%
!! CAAATTCAAATCAAATGTTA+GGG - chr4.1:4043908-4043927 MS.gene048977:CDS 20.0%
!! CATTATTAAAATTTCCAGTT+TGG - chr4.1:4045096-4045115 MS.gene048977:CDS 20.0%
!! TACATGATATTATAAGTTGT+TGG - chr4.1:4045328-4045347 MS.gene048977:CDS 20.0%
!! TCAAATTCAAATCAAATGTT+AGG - chr4.1:4043907-4043926 MS.gene048977:CDS 20.0%
!! TCATGTGAATCTAATTATAA+AGG - chr4.1:4043963-4043982 MS.gene048977:CDS 20.0%
!! TTATAATTAGATTCACATGA+AGG + chr4.1:4043963-4043982 None:intergenic 20.0%
!!! AATATAAGACACCTTTTAAA+TGG - chr4.1:4043602-4043621 MS.gene048977:CDS 20.0%
!!! ATCAAATTTTAAACATGCAA+AGG + chr4.1:4044753-4044772 None:intergenic 20.0%
!!! ATTAGTTTATTACTTATGGT+CGG + chr4.1:4044320-4044339 None:intergenic 20.0%
!!! GCATATTAGTTTATTACTTA+TGG + chr4.1:4044324-4044343 None:intergenic 20.0%
!!! TACCAAAACATATTTTTATG+AGG + chr4.1:4044730-4044749 None:intergenic 20.0%
!!! TGTTTGATTTGCATTTAAAA+AGG - chr4.1:4044796-4044815 MS.gene048977:CDS 20.0%
!!! TTAAAAGGTGTCTTATATTT+TGG + chr4.1:4043601-4043620 None:intergenic 20.0%
!!! TTTAAAAAGGTACCAAAATA+TGG - chr4.1:4044809-4044828 MS.gene048977:CDS 20.0%
!!! TTTTTATTTGCTACACAATT+TGG - chr4.1:4044221-4044240 MS.gene048977:CDS 20.0%
! AATTATAAAGGTAGCAAGTT+GGG - chr4.1:4043975-4043994 MS.gene048977:CDS 25.0%
! ACAGAAACATGTTGAAATAT+AGG - chr4.1:4043521-4043540 MS.gene048977:intron 25.0%
! ATTGAAAATCATCTATCAGT+TGG - chr4.1:4045395-4045414 MS.gene048977:CDS 25.0%
! ATTGATATGAATGCTTATCT+TGG - chr4.1:4045059-4045078 MS.gene048977:CDS 25.0%
! CAACATTACTGTTATACTAT+TGG - chr4.1:4045480-4045499 MS.gene048977:CDS 25.0%
! CAGAAACATGTTGAAATATA+GGG - chr4.1:4043522-4043541 MS.gene048977:intron 25.0%
! GTGTAGCAAATAAAAATAGT+AGG + chr4.1:4044217-4044236 None:intergenic 25.0%
! TAATTATAAAGGTAGCAAGT+TGG - chr4.1:4043974-4043993 MS.gene048977:CDS 25.0%
! TCAAAAGTAGATTGTAATGT+TGG - chr4.1:4043322-4043341 MS.gene048977:CDS 25.0%
! TTCAAAAGGACAATGAATTA+TGG - chr4.1:4044973-4044992 MS.gene048977:intron 25.0%
!! ATGTATTTTGTCCTATGTAA+TGG - chr4.1:4043252-4043271 MS.gene048977:CDS 25.0%
!! CACCTCATAAAAATATGTTT+TGG - chr4.1:4044725-4044744 MS.gene048977:CDS 25.0%
!! CTTTCTTCTTTAAGAATAAG+AGG + chr4.1:4044278-4044297 None:intergenic 25.0%
!! TGTATTTTGTCCTATGTAAT+GGG - chr4.1:4043253-4043272 MS.gene048977:CDS 25.0%
!! TTATGCACTTTTCCATATTT+TGG + chr4.1:4044824-4044843 None:intergenic 25.0%
!! TTGAATTTGAGATGTACAAA+GGG + chr4.1:4043897-4043916 None:intergenic 25.0%
!! TTTGAATTTGAGATGTACAA+AGG + chr4.1:4043898-4043917 None:intergenic 25.0%
!!! AAAATCTAATTTTGAAGACG+AGG + chr4.1:4043383-4043402 None:intergenic 25.0%
!!! CTTTACCCTTTAAAATTACA+AGG - chr4.1:4044677-4044696 MS.gene048977:CDS 25.0%
AAAGGACAATGAATTATGGA+AGG - chr4.1:4044977-4044996 MS.gene048977:intron 30.0%
AGCTACATGATTAGATAAAG+TGG + chr4.1:4045296-4045315 None:intergenic 30.0%
ATTATAAAGGTAGCAAGTTG+GGG - chr4.1:4043976-4043995 MS.gene048977:CDS 30.0%
ATTGAACCACTACTAATTCT+AGG + chr4.1:4044930-4044949 None:intergenic 30.0%
ATTTCTTCCATCTAATAGGT+GGG + chr4.1:4044437-4044456 None:intergenic 30.0%
CAGTATTTCTTCCATCTAAT+AGG + chr4.1:4044441-4044460 None:intergenic 30.0%
CTATTAGATGGAAGAAATAC+TGG - chr4.1:4044439-4044458 MS.gene048977:CDS 30.0%
GAATGAGATCATAAGTTCTA+GGG + chr4.1:4045229-4045248 None:intergenic 30.0%
GCAAATCAAACAGAGAAAAA+TGG + chr4.1:4044788-4044807 None:intergenic 30.0%
TACTGTTATACTATTGGAGA+TGG - chr4.1:4045486-4045505 MS.gene048977:CDS 30.0%
TATTTCTTCCATCTAATAGG+TGG + chr4.1:4044438-4044457 None:intergenic 30.0%
TATTTGGCATATATCAGGAT+AGG - chr4.1:4045261-4045280 MS.gene048977:CDS 30.0%
TCAAGCTGATTATAATGCTT+TGG + chr4.1:4043187-4043206 None:intergenic 30.0%
TCACTTGAAATAAGTACTTC+TGG - chr4.1:4043415-4043434 MS.gene048977:intron 30.0%
TGAATGAGATCATAAGTTCT+AGG + chr4.1:4045230-4045249 None:intergenic 30.0%
TGGTATGTATTTGATTGAAG+TGG - chr4.1:4044371-4044390 MS.gene048977:CDS 30.0%
TTGAAATAAGTACTTCTGGT+GGG - chr4.1:4043419-4043438 MS.gene048977:intron 30.0%
TTGAGAAAGCTGTTCAAAAT+TGG - chr4.1:4043680-4043699 MS.gene048977:CDS 30.0%
TTGTTGCGGAAAAAACTTAT+TGG - chr4.1:4045653-4045672 MS.gene048977:CDS 30.0%
! AAGAATCTTTTCTGGGTTAA+AGG + chr4.1:4045635-4045654 None:intergenic 30.0%
! AATAGACTCACATGGATTTT+AGG - chr4.1:4043217-4043236 MS.gene048977:CDS 30.0%
! ACACATTCAAGGTGATTTTT+AGG + chr4.1:4044585-4044604 None:intergenic 30.0%
! AGAATCTTTTCTGGGTTAAA+GGG + chr4.1:4045634-4045653 None:intergenic 30.0%
! ATCTTTGAAAGAGGTTTCAT+TGG - chr4.1:4045167-4045186 MS.gene048977:CDS 30.0%
! CTATTATGTCCCTTTTCAAA+AGG + chr4.1:4044008-4044027 None:intergenic 30.0%
! TACAAAAGCTCTTCTTTTGT+AGG + chr4.1:4043559-4043578 None:intergenic 30.0%
! TCTTTGAAAGAGGTTTCATT+GGG - chr4.1:4045168-4045187 MS.gene048977:CDS 30.0%
! TTATTTGCTACACAATTTGG+TGG - chr4.1:4044224-4044243 MS.gene048977:CDS 30.0%
!! AACATAATGGATCATGAAAG+TGG - chr4.1:4045607-4045626 MS.gene048977:CDS 30.0%
!! ATTTTTAGGCTTTTGCCAAA+TGG + chr4.1:4044571-4044590 None:intergenic 30.0%
!! CAAAATTGGATTCAAGTTGA+AGG - chr4.1:4043694-4043713 MS.gene048977:CDS 30.0%
!! GTTTAGTTTGTACACATTCA+AGG + chr4.1:4044596-4044615 None:intergenic 30.0%
!!! AAAGGTGTCTTATATTTTGG+AGG + chr4.1:4043598-4043617 None:intergenic 30.0%
AATTCAGCTCATGATACTCT+TGG - chr4.1:4045140-4045159 MS.gene048977:CDS 35.0%
ACAACATTCATAACCCAAAC+TGG + chr4.1:4045113-4045132 None:intergenic 35.0%
AGAAAGATGACCTTTCCATT+TGG - chr4.1:4044553-4044572 MS.gene048977:intron 35.0%
ATCAGAACTGCAATTGATAC+AGG - chr4.1:4043817-4043836 MS.gene048977:CDS 35.0%
CAAGCATAATCTCTACTTTG+AGG + chr4.1:4043628-4043647 None:intergenic 35.0%
CACTGCAGAGAAATTTCAAA+AGG - chr4.1:4044959-4044978 MS.gene048977:intron 35.0%
CGGAAAAAACTTATTGGACT+GGG - chr4.1:4045659-4045678 MS.gene048977:CDS 35.0%
CTATATCTTCCTTGAGCAAT+AGG + chr4.1:4045032-4045051 None:intergenic 35.0%
CTCAAAGTAGAGATTATGCT+TGG - chr4.1:4043626-4043645 MS.gene048977:CDS 35.0%
CTGATATTACCAGTAGTAAG+AGG + chr4.1:4043802-4043821 None:intergenic 35.0%
CTTGAAATAAGTACTTCTGG+TGG - chr4.1:4043418-4043437 MS.gene048977:intron 35.0%
GATATGAATGCTTATCTTGG+TGG - chr4.1:4045062-4045081 MS.gene048977:CDS 35.0%
GGAAAAAACTTATTGGACTG+GGG - chr4.1:4045660-4045679 MS.gene048977:CDS 35.0%
GTATCATGAGCTGAATTTGA+AGG + chr4.1:4045137-4045156 None:intergenic 35.0%
TAGATGGAAGAAATACTGGA+GGG - chr4.1:4044443-4044462 MS.gene048977:CDS 35.0%
TCACTATATGTCATGTCACA+TGG + chr4.1:4043460-4043479 None:intergenic 35.0%
TCAGAACTGCAATTGATACA+GGG - chr4.1:4043818-4043837 MS.gene048977:CDS 35.0%
TGGTGTATTTGGCATATATC+AGG - chr4.1:4045256-4045275 MS.gene048977:CDS 35.0%
TTAGATGGAAGAAATACTGG+AGG - chr4.1:4044442-4044461 MS.gene048977:CDS 35.0%
TTATCAGACTGGCAAATATG+TGG + chr4.1:4044633-4044652 None:intergenic 35.0%
TTTCTTCCAACATATGCGTT+TGG + chr4.1:4044526-4044545 None:intergenic 35.0%
! GAAAAAACTTATTGGACTGG+GGG - chr4.1:4045661-4045680 MS.gene048977:CDS 35.0%
! TATGTCCCTTTTCAAAAGGT+AGG + chr4.1:4044004-4044023 None:intergenic 35.0%
! TCAAATTTTCTACCTCTAGC+TGG + chr4.1:4043502-4043521 None:intergenic 35.0%
!! ACATGGATTTTAGGTGTAAG+TGG - chr4.1:4043226-4043245 MS.gene048977:CDS 35.0%
!! CTACTTTGAGGCCATTTAAA+AGG + chr4.1:4043616-4043635 None:intergenic 35.0%
AACACCTACTAACCAATCTG+AGG - chr4.1:4043294-4043313 MS.gene048977:CDS 40.0%
AAGAGATACACATGAAGCTC+AGG - chr4.1:4044044-4044063 MS.gene048977:intron 40.0%
AAGTTGCCAAACGCATATGT+TGG - chr4.1:4044517-4044536 MS.gene048977:intron 40.0%
AATACATACCATCTGTGCCA+TGG + chr4.1:4044362-4044381 None:intergenic 40.0%
AATACTTACCAAGCCATGTC+AGG + chr4.1:4044657-4044676 None:intergenic 40.0%
AATTTCTCTGCAGTGATGCT+TGG + chr4.1:4044954-4044973 None:intergenic 40.0%
AGAAAGCTTCACACCAATCA+TGG + chr4.1:4045199-4045218 None:intergenic 40.0%
AGATGGAAGAAATACTGGAG+GGG - chr4.1:4044444-4044463 MS.gene048977:CDS 40.0%
ATTCATGCAGCTGGTGTATT+TGG - chr4.1:4045245-4045264 MS.gene048977:CDS 40.0%
CATATCACTCCTATTGCTCA+AGG - chr4.1:4045020-4045039 MS.gene048977:CDS 40.0%
CATATTTGCTCCATGGAATG+AGG + chr4.1:4044176-4044195 None:intergenic 40.0%
CCAAGAGAAATAGACTCACA+TGG - chr4.1:4043209-4043228 MS.gene048977:CDS 40.0%
CCCAGAAAAGATTCTTGTTG+CGG - chr4.1:4045639-4045658 MS.gene048977:CDS 40.0%
CTATATGTCATGTCACATGG+TGG + chr4.1:4043457-4043476 None:intergenic 40.0%
CTCATTCCATGGAGCAAATA+TGG - chr4.1:4044174-4044193 MS.gene048977:intron 40.0%
CTGAATACAACTGTACCTTC+TGG + chr4.1:4045526-4045545 None:intergenic 40.0%
GAAAGAATCCAATAACCACC+TGG + chr4.1:4044411-4044430 None:intergenic 40.0%
GAACCACTACTAATTCTAGG+AGG + chr4.1:4044927-4044946 None:intergenic 40.0%
GAAGTGGAAGAGCAACATAA+TGG - chr4.1:4045594-4045613 MS.gene048977:CDS 40.0%
GAGCTTATTCCTCTTACTAC+TGG - chr4.1:4043790-4043809 MS.gene048977:CDS 40.0%
GCAAGCTTTGCAAATTTCCA+TGG - chr4.1:4044342-4044361 MS.gene048977:CDS 40.0%
GCGGAAAAAACTTATTGGAC+TGG - chr4.1:4045658-4045677 MS.gene048977:CDS 40.0%
GGTACAGTTGTATTCAGAGA+AGG - chr4.1:4045529-4045548 MS.gene048977:CDS 40.0%
GGTGATCCATTGTAATCCTT+GGG + chr4.1:4043166-4043185 None:intergenic 40.0%
GGTGGTTATTGGATTCTTTC+TGG - chr4.1:4044411-4044430 MS.gene048977:CDS 40.0%
GTACCTCCTAGAATTAGTAG+TGG - chr4.1:4044921-4044940 MS.gene048977:intron 40.0%
TACCAACCATATTTGCTCCA+TGG + chr4.1:4044183-4044202 None:intergenic 40.0%
TATGATCTCATTCATGCAGC+TGG - chr4.1:4045236-4045255 MS.gene048977:CDS 40.0%
TCAATGCTAAGCTCTTTGTG+TGG + chr4.1:4043664-4043683 None:intergenic 40.0%
TCATTGGGACTTACCATGAT+TGG - chr4.1:4045183-4045202 MS.gene048977:CDS 40.0%
TCCTGCTATGATTGGTATCA+TGG - chr4.1:4044092-4044111 MS.gene048977:intron 40.0%
TGAGCTTCATGTGTATCTCT+TGG + chr4.1:4044045-4044064 None:intergenic 40.0%
TGGAGATGGATAGAATCTTG+AGG - chr4.1:4045500-4045519 MS.gene048977:CDS 40.0%
TGGCATATATCAGGATAGGT+TGG - chr4.1:4045265-4045284 MS.gene048977:CDS 40.0%
TGGTGATCCATTGTAATCCT+TGG + chr4.1:4043167-4043186 None:intergenic 40.0%
TGTAATGTTGGATCAACAGC+AGG - chr4.1:4043334-4043353 MS.gene048977:CDS 40.0%
TTCCATGGAGCAAATATGGT+TGG - chr4.1:4044178-4044197 MS.gene048977:intron 40.0%
TTGAAGTGGATAGAGTCCTT+AGG - chr4.1:4044385-4044404 MS.gene048977:CDS 40.0%
TTGGTATCATGGCTTCACAA+AGG - chr4.1:4044103-4044122 MS.gene048977:intron 40.0%
TTTCCATGCACCCATTACAT+AGG + chr4.1:4043266-4043285 None:intergenic 40.0%
! CCATGTGAGTCTATTTCTCT+TGG + chr4.1:4043212-4043231 None:intergenic 40.0%
! CCGCAACAAGAATCTTTTCT+GGG + chr4.1:4045642-4045661 None:intergenic 40.0%
! CTTGGTGCAATCTTTGAAAG+AGG - chr4.1:4045158-4045177 MS.gene048977:CDS 40.0%
! GTGTTACTGATGCAATGAAG+TGG - chr4.1:4045578-4045597 MS.gene048977:CDS 40.0%
! TAGGCTTTTGCCAAATGGAA+AGG + chr4.1:4044566-4044585 None:intergenic 40.0%
! TATGCCTCAGATTGGTTAGT+AGG + chr4.1:4043301-4043320 None:intergenic 40.0%
! TCCGCAACAAGAATCTTTTC+TGG + chr4.1:4045643-4045662 None:intergenic 40.0%
!! AATTCTAGGAGGTACTGCAA+AGG + chr4.1:4044916-4044935 None:intergenic 40.0%
!! CTTTTGAGTATGCCTCAGAT+TGG + chr4.1:4043309-4043328 None:intergenic 40.0%
!! GGATTCAAGTTGAAGGTGAT+CGG - chr4.1:4043701-4043720 MS.gene048977:CDS 40.0%
!! GTCTTATATTTTGGAGGAGC+AGG + chr4.1:4043592-4043611 None:intergenic 40.0%
ACTGCAATTGATACAGGGTG+TGG - chr4.1:4043823-4043842 MS.gene048977:CDS 45.0%
AGAGGAGTTCCTGCTATGAT+TGG - chr4.1:4044084-4044103 MS.gene048977:intron 45.0%
AGCTCAGGTACAGTTTGCAT+TGG - chr4.1:4044059-4044078 MS.gene048977:intron 45.0%
CCAGTCTGATAATCCTGACA+TGG - chr4.1:4044641-4044660 MS.gene048977:intron 45.0%
CCATATCAAAAGCCCTTGCT+GGG + chr4.1:4044137-4044156 None:intergenic 45.0%
CCATGTCAGGATTATCAGAC+TGG + chr4.1:4044644-4044663 None:intergenic 45.0%
CGAGGACGATGATGAAATTG+AGG + chr4.1:4043365-4043384 None:intergenic 45.0%
CTGATAATCCTGACATGGCT+TGG - chr4.1:4044646-4044665 MS.gene048977:intron 45.0%
CTGGAGGGGAACTAGAAAAA+TGG - chr4.1:4044880-4044899 MS.gene048977:intron 45.0%
GAGCTGGTAACTTCTGGTAA+TGG + chr4.1:4044849-4044868 None:intergenic 45.0%
GATAGAATCTTGAGGCCAGA+AGG - chr4.1:4045508-4045527 MS.gene048977:CDS 45.0%
GCAAATTTCCATGGCACAGA+TGG - chr4.1:4044351-4044370 MS.gene048977:CDS 45.0%
GCAAGAGCAAGATTCCATAG+AGG - chr4.1:4044494-4044513 MS.gene048977:intron 45.0%
GCCATGATACCAATCATAGC+AGG + chr4.1:4044096-4044115 None:intergenic 45.0%
GTACAGTTTGCATTGGAGAG+AGG - chr4.1:4044066-4044085 MS.gene048977:intron 45.0%
TTGCTTGAGCTGGTAACTTC+TGG + chr4.1:4044855-4044874 None:intergenic 45.0%
! TTTCTGGCCCACCTATTAGA+TGG - chr4.1:4044427-4044446 MS.gene048977:CDS 45.0%
!! GGGTGCCTACCTTTTGAAAA+GGG - chr4.1:4043996-4044015 MS.gene048977:CDS 45.0%
!! TGTCCTATGTAATGGGTGCA+TGG - chr4.1:4043260-4043279 MS.gene048977:CDS 45.0%
AAAGGCTACCATACCCAGCA+AGG - chr4.1:4044121-4044140 MS.gene048977:intron 50.0%
AAGGCTACCATACCCAGCAA+GGG - chr4.1:4044122-4044141 MS.gene048977:intron 50.0%
ACCAGCTCAAGCAAAGTAGC+TGG - chr4.1:4044861-4044880 MS.gene048977:CDS 50.0%
AGCTCAAGCAAAGTAGCTGG+AGG - chr4.1:4044864-4044883 MS.gene048977:intron 50.0%
CCATGTCAAGATCCAGCTAG+AGG - chr4.1:4043487-4043506 MS.gene048977:intron 50.0%
CCTCTAGCTGGATCTTGACA+TGG + chr4.1:4043490-4043509 None:intergenic 50.0%
CTCAAGCAAAGTAGCTGGAG+GGG - chr4.1:4044866-4044885 MS.gene048977:intron 50.0%
GAAACATGGTACCTCCACCA+GGG + chr4.1:4043735-4043754 None:intergenic 50.0%
GAAGAAATACTGGAGGGGCT+GGG - chr4.1:4044449-4044468 MS.gene048977:CDS 50.0%
GCCATATCAAAAGCCCTTGC+TGG + chr4.1:4044138-4044157 None:intergenic 50.0%
GCGTTTGGCAACTTCCTCTA+TGG + chr4.1:4044511-4044530 None:intergenic 50.0%
GCTCAAGCAAAGTAGCTGGA+GGG - chr4.1:4044865-4044884 MS.gene048977:intron 50.0%
TCAAAAGCCCTTGCTGGGTA+TGG + chr4.1:4044132-4044151 None:intergenic 50.0%
TCCAATAACCACCTGGCCTA+AGG + chr4.1:4044404-4044423 None:intergenic 50.0%
TCCTTAGGCCAGGTGGTTAT+TGG - chr4.1:4044400-4044419 MS.gene048977:CDS 50.0%
! CCCAGCAAGGGCTTTTGATA+TGG - chr4.1:4044134-4044153 MS.gene048977:intron 50.0%
! TCCAGCTACTTTGCTTGAGC+TGG + chr4.1:4044865-4044884 None:intergenic 50.0%
!! GGGGTGCCTACCTTTTGAAA+AGG - chr4.1:4043995-4044014 MS.gene048977:CDS 50.0%
ATCAGCACCGCGAGGAAACA+TGG + chr4.1:4043749-4043768 None:intergenic 55.0%
GATAGAGTCCTTAGGCCAGG+TGG - chr4.1:4044393-4044412 MS.gene048977:CDS 55.0%
GGAAACATGGTACCTCCACC+AGG + chr4.1:4043736-4043755 None:intergenic 55.0%
GGAAGAAATACTGGAGGGGC+TGG - chr4.1:4044448-4044467 MS.gene048977:CDS 55.0%
GGGCTGGGAAAGAACTGAAG+AGG - chr4.1:4044464-4044483 MS.gene048977:CDS 55.0%
GTGGATAGAGTCCTTAGGCC+AGG - chr4.1:4044390-4044409 MS.gene048977:CDS 55.0%
GTTCTCGCTGCCTCATTCCA+TGG - chr4.1:4044163-4044182 MS.gene048977:intron 55.0%
! GATCGGTTTCGATTCCCTGG+TGG - chr4.1:4043718-4043737 MS.gene048977:CDS 55.0%
! GGTACTGCAAAGGCACGTCT+TGG + chr4.1:4044906-4044925 None:intergenic 55.0%
! GGTGATCGGTTTCGATTCCC+TGG - chr4.1:4043715-4043734 MS.gene048977:CDS 55.0%
!! GGAGGTACCATGTTTCCTCG+CGG - chr4.1:4043739-4043758 MS.gene048977:CDS 55.0%
ATGTACGCATCAGCACCGCG+AGG + chr4.1:4043757-4043776 None:intergenic 60.0%
CGGTTTCGATTCCCTGGTGG+AGG - chr4.1:4043721-4043740 MS.gene048977:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 4043160 4045705 4043160 ID=MS.gene048977
chr4.1 mRNA 4043160 4045705 4043160 ID=MS.gene048977.t1;Parent=MS.gene048977
chr4.1 exon 4045019 4045705 4045019 ID=MS.gene048977.t1.exon1;Parent=MS.gene048977.t1
chr4.1 CDS 4045019 4045705 4045019 ID=cds.MS.gene048977.t1;Parent=MS.gene048977.t1
chr4.1 exon 4044670 4044880 4044670 ID=MS.gene048977.t1.exon2;Parent=MS.gene048977.t1
chr4.1 CDS 4044670 4044880 4044670 ID=cds.MS.gene048977.t1;Parent=MS.gene048977.t1
chr4.1 exon 4044198 4044495 4044198 ID=MS.gene048977.t1.exon3;Parent=MS.gene048977.t1
chr4.1 CDS 4044198 4044495 4044198 ID=cds.MS.gene048977.t1;Parent=MS.gene048977.t1
chr4.1 exon 4043583 4044047 4043583 ID=MS.gene048977.t1.exon4;Parent=MS.gene048977.t1
chr4.1 CDS 4043583 4044047 4043583 ID=cds.MS.gene048977.t1;Parent=MS.gene048977.t1
chr4.1 exon 4043160 4043388 4043160 ID=MS.gene048977.t1.exon5;Parent=MS.gene048977.t1
chr4.1 CDS 4043160 4043388 4043160 ID=cds.MS.gene048977.t1;Parent=MS.gene048977.t1
Gene Sequence

>MS.gene048977

ATGCCCAAGGATTACAATGGATCACCAAAGCATTATAATCAGCTTGAATCCAAGAGAAATAGACTCACATGGATTTTAGGTGTAAGTGGTCTATGTATTTTGTCCTATGTAATGGGTGCATGGAAAAATACAGCAACACCTACTAACCAATCTGAGGCATACTCAAAAGTAGATTGTAATGTTGGATCAACAGCAGGTTCTTCCTCAATTTCATCATCGTCCTCGTCTTCAAAATTAGATTTTGCTAGTCATCATTCACTTGAAATAAGTACTTCTGGTGGGATAAAAGAGTTTCCACCATGTGACATGACATATAGTGAATACACACCATGTCAAGATCCAGCTAGAGGTAGAAAATTTGACAGAAACATGTTGAAATATAGGGAGAGACATTGTCCTACAAAAGAAGAGCTTTTGTATTGTCTTATACCTGCTCCTCCAAAATATAAGACACCTTTTAAATGGCCTCAAAGTAGAGATTATGCTTGGTATGATAATATTCCACACAAAGAGCTTAGCATTGAGAAAGCTGTTCAAAATTGGATTCAAGTTGAAGGTGATCGGTTTCGATTCCCTGGTGGAGGTACCATGTTTCCTCGCGGTGCTGATGCGTACATTGATGACATTAATGAGCTTATTCCTCTTACTACTGGTAATATCAGAACTGCAATTGATACAGGGTGTGGTGTAAGTTCATTCATCTATACACTTAATCATGATTTGTTTTATGTTTTCCCTTTGTACATCTCAAATTCAAATCAAATGTTAGGGTTATGCATATGTTGCTAACACTTTATATACCTTCATGTGAATCTAATTATAAAGGTAGCAAGTTGGGGTGCCTACCTTTTGAAAAGGGACATAATAGCAATGTCATTTGCACCAAGAGATACACATGAAGCTCAGGTACAGTTTGCATTGGAGAGAGGAGTTCCTGCTATGATTGGTATCATGGCTTCACAAAGGCTACCATACCCAGCAAGGGCTTTTGATATGGCTCATTGTTCTCGCTGCCTCATTCCATGGAGCAAATATGGTTGGTATTCACACTTCTCCTACTATTTTTATTTGCTACACAATTTGGTGGTATTTTGCAATTAAGAGACATTTTAAGTCCTCTTATTCTTAAAGAAGAAAGAATTAAAAAAATTCTTATGCCGACCATAAGTAATAAACTAATATGCAAGCTTTGCAAATTTCCATGGCACAGATGGTATGTATTTGATTGAAGTGGATAGAGTCCTTAGGCCAGGTGGTTATTGGATTCTTTCTGGCCCACCTATTAGATGGAAGAAATACTGGAGGGGCTGGGAAAGAACTGAAGAGGATTTAAAGCAAGAGCAAGATTCCATAGAGGAAGTTGCCAAACGCATATGTTGGAAGAAAGTTGTTGAGAAAGATGACCTTTCCATTTGGCAAAAGCCTAAAAATCACCTTGAATGTGTACAAACTAAACAAGTCTTTAAAACACCACATATTTGCCAGTCTGATAATCCTGACATGGCTTGGTAAGTATTCTTTACCCTTTAAAATTACAAGGTTAAATTCTCTTAGCAGTGAACTTACACCTCATAAAAATATGTTTTGGTACCTTTGCATGTTTAAAATTTGATATATTTTGTTTACCATTTTTCTCTGTTTGATTTGCATTTAAAAAGGTACCAAAATATGGAAAAGTGCATAACTCCATTACCAGAAGTTACCAGCTCAAGCAAAGTAGCTGGAGGGGAACTAGAAAAATGGCCAAGACGTGCCTTTGCAGTACCTCCTAGAATTAGTAGTGGTTCAATACCAAGCATCACTGCAGAGAAATTTCAAAAGGACAATGAATTATGGAAGGACAGAATGTCACATTACAAGCATATCACTCCTATTGCTCAAGGAAGATATAGAAACATTATTGATATGAATGCTTATCTTGGTGGATTTGCAGCAGCATTATTAAAATTTCCAGTTTGGGTTATGAATGTTGTTCCTTCAAATTCAGCTCATGATACTCTTGGTGCAATCTTTGAAAGAGGTTTCATTGGGACTTACCATGATTGGTGTGAAGCTTTCTCAACTTACCCTAGAACTTATGATCTCATTCATGCAGCTGGTGTATTTGGCATATATCAGGATAGGTTGGTATTTCCACTTTATCTAATCATGTAGCTTTAATAATTTGTTACATGATATTATAAGTTGTTGGTGAAGTGCAGTATAGTCTCTAAAATCAAAGAAATGATTCTTGAAATTGAAAATCATCTATCAGTTGGTATCATTTCTATATGTTTTATCATGTGTTTTTGACTGAATTAATTGTTATGTATGGAAGGTGCAACATTACTGTTATACTATTGGAGATGGATAGAATCTTGAGGCCAGAAGGTACAGTTGTATTCAGAGAAGGAGTAGAGCTTCTCACAAAGATTAAAAGTGTTACTGATGCAATGAAGTGGAAGAGCAACATAATGGATCATGAAAGTGGACCCTTTAACCCAGAAAAGATTCTTGTTGCGGAAAAAACTTATTGGACTGGGGGAGCTAAAGATAATAGCAACTAA
Protein sequence

>MS.gene048977.t1

MPKDYNGSPKHYNQLESKRNRLTWILGVSGLCILSYVMGAWKNTATPTNQSEAYSKVDCNVGSTAGSSSISSSSSSSKLDFASHHSLEISTSGGIKEFPPCDMTYSEYTPCQDPARGRKFDRNMLKYRERHCPTKEELLYCLIPAPPKYKTPFKWPQSRDYAWYDNIPHKELSIEKAVQNWIQVEGDRFRFPGGGTMFPRGADAYIDDINELIPLTTGNIRTAIDTGCGVASWGAYLLKRDIIAMSFAPRDTHEAQVQFALERGVPAMIGIMASQRLPYPARAFDMAHCSRCLIPWSKYDGMYLIEVDRVLRPGGYWILSGPPIRWKKYWRGWERTEEDLKQEQDSIEEVAKRICWKKVVEKDDLSIWQKPKNHLECVQTKQVFKTPHICQSDNPDMAWYQNMEKCITPLPEVTSSSKVAGGELEKWPRRAFAVPPRISSGSIPSITAEKFQKDNELWKDRMSHYKHITPIAQGRYRNIIDMNAYLGGFAAALLKFPVWVMNVVPSNSAHDTLGAIFERGFIGTYHDWCEAFSTYPRTYDLIHAAGVFGIYQDRCNITVILLEMDRILRPEGTVVFREGVELLTKIKSVTDAMKWKSNIMDHESGPFNPEKILVAEKTYWTGGAKDNSN