Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051307.t1 | XP_003593643.1 | 98.2 | 55 | 1 | 0 | 1 | 55 | 1 | 55 | 7.70E-23 | 115.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051307.t1 | A9IGC5 | 80.8 | 52 | 9 | 1 | 4 | 55 | 5 | 55 | 7.8e-16 | 83.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051307.t1 | A2Q503 | 98.2 | 55 | 1 | 0 | 1 | 55 | 1 | 55 | 5.6e-23 | 115.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene051307 | 0.82547 | 4.61E-54 | -1.69E-46 |
| MS.gene051307 | MS.gene054236 | 0.808637 | 2.81E-50 | -1.69E-46 |
| MS.gene051307 | MS.gene072098 | 0.803244 | 3.82E-49 | -1.69E-46 |
| MS.gene051307 | MS.gene073139 | 0.825733 | 3.99E-54 | -1.69E-46 |
| MS.gene051307 | MS.gene073753 | 0.800467 | 1.42E-48 | -1.69E-46 |
| MS.gene051307 | MS.gene07532 | 0.82621 | 3.07E-54 | -1.69E-46 |
| MS.gene051307 | MS.gene43973 | 0.807954 | 3.93E-50 | -1.69E-46 |
| MS.gene051307 | MS.gene50639 | 0.806031 | 1.00E-49 | -1.69E-46 |
| MS.gene051307 | MS.gene54876 | 0.803187 | 3.93E-49 | -1.69E-46 |
| MS.gene051307 | MS.gene58547 | 0.801665 | 8.09E-49 | -1.69E-46 |
| MS.gene051307 | MS.gene58802 | 0.802523 | 5.39E-49 | -1.69E-46 |
| MS.gene051307 | MS.gene60903 | 0.808001 | 3.84E-50 | -1.69E-46 |
| MS.gene051307 | MS.gene69794 | 0.800481 | 1.41E-48 | -1.69E-46 |
| MS.gene051307 | MS.gene90760 | 0.803048 | 4.20E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051307.t1 | MTR_2g014480 | 98.182 | 55 | 1 | 0 | 1 | 55 | 1 | 55 | 5.54e-33 | 115 |
| MS.gene051307.t1 | MTR_4g078850 | 83.636 | 55 | 9 | 0 | 1 | 55 | 1 | 55 | 4.39e-27 | 99.8 |
| MS.gene051307.t1 | MTR_2g014470 | 81.818 | 55 | 10 | 0 | 1 | 55 | 1 | 55 | 1.96e-26 | 97.8 |
| MS.gene051307.t1 | MTR_4g015840 | 74.545 | 55 | 14 | 0 | 1 | 55 | 1 | 55 | 1.31e-25 | 95.5 |
| MS.gene051307.t1 | MTR_3g108020 | 77.778 | 54 | 12 | 0 | 1 | 54 | 1 | 54 | 2.98e-25 | 94.4 |
| MS.gene051307.t1 | MTR_2g090705 | 74.545 | 55 | 14 | 0 | 1 | 55 | 1 | 55 | 1.77e-24 | 92.4 |
| MS.gene051307.t1 | MTR_7g101070 | 55.769 | 52 | 23 | 0 | 4 | 55 | 358 | 409 | 3.98e-16 | 70.1 |
| MS.gene051307.t1 | MTR_7g109340 | 59.615 | 52 | 20 | 1 | 4 | 55 | 26 | 76 | 3.10e-15 | 67.4 |
| MS.gene051307.t1 | MTR_7g112360 | 45.283 | 53 | 26 | 1 | 5 | 54 | 17 | 69 | 1.81e-12 | 57.0 |
| MS.gene051307.t1 | MTR_7g112360 | 45.283 | 53 | 26 | 1 | 5 | 54 | 17 | 69 | 1.98e-12 | 58.2 |
| MS.gene051307.t1 | MTR_3g087030 | 57.692 | 52 | 20 | 1 | 4 | 55 | 82 | 131 | 2.13e-12 | 59.7 |
| MS.gene051307.t1 | MTR_3g437850 | 48.148 | 54 | 27 | 1 | 3 | 55 | 1 | 54 | 3.25e-12 | 57.4 |
| MS.gene051307.t1 | MTR_5g037880 | 50.847 | 59 | 22 | 1 | 4 | 55 | 5 | 63 | 6.38e-12 | 58.2 |
| MS.gene051307.t1 | MTR_5g065860 | 51.923 | 52 | 24 | 1 | 4 | 55 | 48 | 98 | 7.12e-12 | 56.2 |
| MS.gene051307.t1 | MTR_5g065160 | 50.000 | 52 | 25 | 1 | 4 | 55 | 83 | 133 | 2.24e-11 | 56.6 |
| MS.gene051307.t1 | MTR_6g027110 | 40.351 | 57 | 30 | 1 | 3 | 55 | 1 | 57 | 2.28e-11 | 55.1 |
| MS.gene051307.t1 | MTR_5g065160 | 50.000 | 52 | 25 | 1 | 4 | 55 | 83 | 133 | 2.42e-11 | 56.6 |
| MS.gene051307.t1 | MTR_5g065160 | 50.000 | 52 | 25 | 1 | 4 | 55 | 82 | 132 | 2.44e-11 | 56.6 |
| MS.gene051307.t1 | MTR_5g065160 | 50.000 | 52 | 25 | 1 | 4 | 55 | 76 | 126 | 2.59e-11 | 56.6 |
| MS.gene051307.t1 | MTR_3g027580 | 50.000 | 52 | 25 | 1 | 4 | 55 | 89 | 139 | 3.05e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051307.t1 | AT5G01390 | 74.545 | 55 | 14 | 0 | 1 | 55 | 1 | 55 | 9.79e-26 | 94.0 |
| MS.gene051307.t1 | AT5G01390 | 74.545 | 55 | 14 | 0 | 1 | 55 | 1 | 55 | 2.22e-25 | 94.0 |
| MS.gene051307.t1 | AT5G01390 | 74.545 | 55 | 14 | 0 | 1 | 55 | 1 | 55 | 2.56e-25 | 92.8 |
| MS.gene051307.t1 | AT4G28480 | 74.545 | 55 | 14 | 0 | 1 | 55 | 1 | 55 | 4.32e-25 | 93.6 |
| MS.gene051307.t1 | AT2G20560 | 74.545 | 55 | 14 | 0 | 1 | 55 | 1 | 55 | 4.95e-25 | 94.0 |
| MS.gene051307.t1 | AT5G25530 | 74.545 | 55 | 14 | 0 | 1 | 55 | 1 | 55 | 7.92e-25 | 93.6 |
| MS.gene051307.t1 | AT4G28480 | 74.545 | 55 | 14 | 0 | 1 | 55 | 1 | 55 | 9.54e-25 | 93.6 |
| MS.gene051307.t1 | AT5G25530 | 74.545 | 55 | 14 | 0 | 1 | 55 | 1 | 55 | 9.63e-25 | 93.6 |
| MS.gene051307.t1 | AT3G08910 | 76.364 | 55 | 13 | 0 | 1 | 55 | 1 | 55 | 1.50e-24 | 92.4 |
| MS.gene051307.t1 | AT5G01390 | 74.545 | 55 | 14 | 0 | 1 | 55 | 1 | 55 | 4.19e-24 | 91.7 |
| MS.gene051307.t1 | AT1G10350 | 63.636 | 55 | 20 | 0 | 1 | 55 | 1 | 55 | 1.70e-21 | 84.7 |
| MS.gene051307.t1 | AT1G59725 | 64.286 | 56 | 19 | 1 | 1 | 55 | 1 | 56 | 7.88e-20 | 80.1 |
| MS.gene051307.t1 | AT3G47940 | 66.071 | 56 | 18 | 1 | 1 | 55 | 1 | 56 | 9.58e-20 | 80.1 |
| MS.gene051307.t1 | AT5G03160 | 51.923 | 52 | 25 | 0 | 4 | 55 | 370 | 421 | 2.21e-14 | 65.5 |
| MS.gene051307.t1 | AT2G41000 | 45.614 | 57 | 27 | 1 | 3 | 55 | 2 | 58 | 1.52e-13 | 61.6 |
| MS.gene051307.t1 | AT2G22360 | 59.615 | 52 | 19 | 1 | 4 | 55 | 86 | 135 | 6.09e-13 | 61.2 |
| MS.gene051307.t1 | AT5G48030 | 55.769 | 52 | 22 | 1 | 4 | 55 | 94 | 144 | 1.03e-12 | 60.5 |
| MS.gene051307.t1 | AT3G62600 | 51.852 | 54 | 25 | 1 | 2 | 55 | 24 | 76 | 1.19e-12 | 60.5 |
| MS.gene051307.t1 | AT1G56300 | 47.273 | 55 | 26 | 1 | 4 | 55 | 13 | 67 | 1.35e-12 | 57.4 |
| MS.gene051307.t1 | AT4G39960 | 57.692 | 52 | 20 | 1 | 4 | 55 | 85 | 134 | 1.40e-12 | 60.1 |
| MS.gene051307.t1 | AT4G39960 | 57.692 | 52 | 20 | 1 | 4 | 55 | 85 | 134 | 1.40e-12 | 60.1 |
| MS.gene051307.t1 | AT1G56300 | 48.148 | 54 | 25 | 1 | 5 | 55 | 14 | 67 | 1.70e-12 | 58.2 |
| MS.gene051307.t1 | AT3G17830 | 55.357 | 56 | 19 | 2 | 2 | 55 | 61 | 112 | 1.16e-11 | 57.8 |
| MS.gene051307.t1 | AT5G16650 | 54.902 | 51 | 22 | 1 | 4 | 54 | 11 | 60 | 1.95e-11 | 54.7 |
| MS.gene051307.t1 | AT5G16650 | 54.902 | 51 | 22 | 1 | 4 | 54 | 11 | 60 | 2.38e-11 | 55.5 |
| MS.gene051307.t1 | AT2G41000 | 45.614 | 57 | 27 | 1 | 3 | 55 | 2 | 58 | 3.46e-11 | 55.1 |
| MS.gene051307.t1 | AT1G59980 | 50.000 | 50 | 24 | 1 | 6 | 55 | 25 | 73 | 7.67e-11 | 55.5 |
Find 9 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGGATTACTATGAGATTT+TGG | 0.205034 | 5.4:-19982351 | MS.gene051307:CDS |
| TTATTGTCAGGATTCTTATC+AGG | 0.253732 | 5.4:+19982260 | None:intergenic |
| CTTATCAGGGTGCCATTTCA+TGG | 0.338061 | 5.4:+19982274 | None:intergenic |
| ATAGAAAACTTGCCATGAAA+TGG | 0.412382 | 5.4:-19982286 | MS.gene051307:CDS |
| TCAGCATCATTCTTATTGTC+AGG | 0.435149 | 5.4:+19982248 | None:intergenic |
| GACTAAATTCAAACTCATAT+CGG | 0.437957 | 5.4:-19982225 | MS.gene051307:CDS |
| TATTGTCAGGATTCTTATCA+GGG | 0.462954 | 5.4:+19982261 | None:intergenic |
| AAAGTAGTGGAAGATGGGTT+TGG | 0.472595 | 5.4:-19982369 | None:intergenic |
| ACTCATATCGGAAGCATATG+AGG | 0.475266 | 5.4:-19982213 | MS.gene051307:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GACTAAATTCAAACTCATAT+CGG | - | chr5.4:19982170-19982189 | MS.gene051307:intron | 25.0% |
| ! | GTTTAATTGCTACAAAACTA+TGG | - | chr5.4:19982235-19982254 | MS.gene051307:CDS | 25.0% |
| !! | TTTGGATTACTATGAGATTT+TGG | - | chr5.4:19982044-19982063 | MS.gene051307:intron | 25.0% |
| !!! | ATTATCTTGTTTCTCTGTTT+TGG | - | chr5.4:19982305-19982324 | MS.gene051307:CDS | 25.0% |
| ATAGAAAACTTGCCATGAAA+TGG | - | chr5.4:19982109-19982128 | MS.gene051307:intron | 30.0% | |
| TATTGTCAGGATTCTTATCA+GGG | + | chr5.4:19982137-19982156 | None:intergenic | 30.0% | |
| TTATTGTCAGGATTCTTATC+AGG | + | chr5.4:19982138-19982157 | None:intergenic | 30.0% | |
| !! | TGTTTACTGTCTTTTGTTTG+AGG | - | chr5.4:19982351-19982370 | MS.gene051307:CDS | 30.0% |
| TCAGCATCATTCTTATTGTC+AGG | + | chr5.4:19982150-19982169 | None:intergenic | 35.0% | |
| ! | ACTGATCATTCATGTTTCTG+AGG | - | chr5.4:19982261-19982280 | MS.gene051307:CDS | 35.0% |
| ! | GGATTACTATGAGATTTTGG+AGG | - | chr5.4:19982047-19982066 | MS.gene051307:intron | 35.0% |
| ! | ACTCATATCGGAAGCATATG+AGG | - | chr5.4:19982182-19982201 | MS.gene051307:intron | 40.0% |
| !! | CTTATCAGGGTGCCATTTCA+TGG | + | chr5.4:19982124-19982143 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 19982039 | 19982378 | 19982039 | ID=MS.gene051307 |
| chr5.4 | mRNA | 19982039 | 19982378 | 19982039 | ID=MS.gene051307.t1;Parent=MS.gene051307 |
| chr5.4 | exon | 19982214 | 19982378 | 19982214 | ID=MS.gene051307.t1.exon1;Parent=MS.gene051307.t1 |
| chr5.4 | CDS | 19982214 | 19982378 | 19982214 | ID=cds.MS.gene051307.t1;Parent=MS.gene051307.t1 |
| chr5.4 | exon | 19982039 | 19982044 | 19982039 | ID=MS.gene051307.t1.exon2;Parent=MS.gene051307.t1 |
| chr5.4 | CDS | 19982039 | 19982044 | 19982039 | ID=cds.MS.gene051307.t1;Parent=MS.gene051307.t1 |
| Gene Sequence |
| Protein sequence |
