AlfalfaGEDB | MS.gene90760

M. sativa cultivar XinJiangDaYe / MS.gene90760

KEGG: K14962
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90760.t1	XP_013446074.1	100	66	0	0	1	66	1	66	2.10E-27	131

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90760.t1	Q9LYK6	42.0	50	29	0	15	64	5	54	6.6e-06	50.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90760.t1	B7FLB2	100.0	66	0	0	1	66	1	66	1.5e-27	131.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene90760	0.814084	1.84E-51	-1.69E-46
MS.gene049159	MS.gene90760	0.871554	6.26E-67	-1.69E-46
MS.gene049254	MS.gene90760	0.811874	5.63E-51	-1.69E-46
MS.gene049404	MS.gene90760	0.810869	9.30E-51	-1.69E-46
MS.gene049435	MS.gene90760	0.823712	1.19E-53	-1.69E-46
MS.gene049452	MS.gene90760	0.815638	8.34E-52	-1.69E-46
MS.gene049501	MS.gene90760	0.825575	4.35E-54	-1.69E-46
MS.gene049821	MS.gene90760	0.803364	3.61E-49	-1.69E-46
MS.gene049983	MS.gene90760	0.804056	2.59E-49	-1.69E-46
MS.gene050020	MS.gene90760	0.811139	8.13E-51	-1.69E-46
MS.gene050273	MS.gene90760	0.809144	2.19E-50	-1.69E-46
MS.gene050442	MS.gene90760	0.829008	6.55E-55	-1.69E-46
MS.gene050680	MS.gene90760	0.829255	5.70E-55	-1.69E-46
MS.gene050759	MS.gene90760	0.822764	1.99E-53	-1.69E-46
MS.gene050780	MS.gene90760	0.813211	2.87E-51	-1.69E-46
MS.gene050807	MS.gene90760	0.830218	3.32E-55	-1.69E-46
MS.gene050815	MS.gene90760	0.801836	7.46E-49	-1.69E-46
MS.gene050826	MS.gene90760	0.833854	4.20E-56	-1.69E-46
MS.gene050953	MS.gene90760	0.803991	2.68E-49	-1.69E-46
MS.gene050962	MS.gene90760	0.819688	1.02E-52	-1.69E-46
MS.gene051198	MS.gene90760	0.804494	2.10E-49	-1.69E-46
MS.gene051243	MS.gene90760	0.806217	9.15E-50	-1.69E-46
MS.gene051247	MS.gene90760	0.820971	5.17E-53	-1.69E-46
MS.gene051307	MS.gene90760	0.803048	4.20E-49	-1.69E-46
MS.gene051328	MS.gene90760	0.808831	2.55E-50	-1.69E-46
MS.gene051719	MS.gene90760	0.800973	1.12E-48	-1.69E-46
MS.gene051720	MS.gene90760	0.803941	2.74E-49	-1.69E-46
MS.gene051744	MS.gene90760	0.807887	4.06E-50	-1.69E-46
MS.gene051800	MS.gene90760	0.828309	9.66E-55	-1.69E-46
MS.gene052012	MS.gene90760	0.822528	2.26E-53	-1.69E-46
MS.gene052020	MS.gene90760	0.814646	1.39E-51	-1.69E-46
MS.gene05215	MS.gene90760	0.802874	4.56E-49	-1.69E-46
MS.gene052323	MS.gene90760	0.800117	1.67E-48	-1.69E-46
MS.gene052426	MS.gene90760	0.81658	5.14E-52	-1.69E-46
MS.gene052516	MS.gene90760	0.829	6.57E-55	-1.69E-46
MS.gene052519	MS.gene90760	0.816532	5.27E-52	-1.69E-46
MS.gene052611	MS.gene90760	0.805771	1.14E-49	-1.69E-46
MS.gene052674	MS.gene90760	0.806813	6.85E-50	-1.69E-46
MS.gene052675	MS.gene90760	0.808632	2.81E-50	-1.69E-46
MS.gene052677	MS.gene90760	0.811461	6.92E-51	-1.69E-46
MS.gene052773	MS.gene90760	0.818281	2.13E-52	-1.69E-46
MS.gene052863	MS.gene90760	0.835372	1.74E-56	-1.69E-46
MS.gene052896	MS.gene90760	0.811916	5.51E-51	-1.69E-46
MS.gene053008	MS.gene90760	0.840868	6.71E-58	-1.69E-46
MS.gene053166	MS.gene90760	0.831096	2.03E-55	-1.69E-46
MS.gene053226	MS.gene90760	0.823516	1.33E-53	-1.69E-46
MS.gene05415	MS.gene90760	0.847728	9.63E-60	-1.69E-46
MS.gene054236	MS.gene90760	0.827523	1.49E-54	-1.69E-46
MS.gene054467	MS.gene90760	0.807143	5.84E-50	-1.69E-46
MS.gene05462	MS.gene90760	0.810852	9.38E-51	-1.69E-46
MS.gene054651	MS.gene90760	0.82172	3.47E-53	-1.69E-46
MS.gene05468	MS.gene90760	0.803645	3.16E-49	-1.69E-46
MS.gene054775	MS.gene90760	0.823239	1.54E-53	-1.69E-46
MS.gene05510	MS.gene90760	0.838012	3.70E-57	-1.69E-46
MS.gene05517	MS.gene90760	0.813615	2.34E-51	-1.69E-46
MS.gene055227	MS.gene90760	0.835023	2.14E-56	-1.69E-46
MS.gene055512	MS.gene90760	0.806894	6.59E-50	-1.69E-46
MS.gene055623	MS.gene90760	0.843867	1.08E-58	-1.69E-46
MS.gene055701	MS.gene90760	0.823078	1.68E-53	-1.69E-46
MS.gene055818	MS.gene90760	0.83033	3.12E-55	-1.69E-46
MS.gene055926	MS.gene90760	0.867793	1.05E-65	-1.69E-46
MS.gene05604	MS.gene90760	0.825434	4.70E-54	-1.69E-46
MS.gene056173	MS.gene90760	0.839311	1.71E-57	-1.69E-46
MS.gene056174	MS.gene90760	0.832701	8.13E-56	-1.69E-46
MS.gene056308	MS.gene90760	0.800778	1.23E-48	-1.69E-46
MS.gene056480	MS.gene90760	0.804535	2.06E-49	-1.69E-46
MS.gene056506	MS.gene90760	0.820792	5.69E-53	-1.69E-46
MS.gene056760	MS.gene90760	0.807433	5.07E-50	-1.69E-46
MS.gene056873	MS.gene90760	0.800066	1.72E-48	-1.69E-46
MS.gene057060	MS.gene90760	0.813604	2.35E-51	-1.69E-46
MS.gene057208	MS.gene90760	0.817506	3.18E-52	-1.69E-46
MS.gene057444	MS.gene90760	0.814707	1.34E-51	-1.69E-46
MS.gene057693	MS.gene90760	0.843012	1.82E-58	-1.69E-46
MS.gene057855	MS.gene90760	0.821743	3.43E-53	-1.69E-46
MS.gene057869	MS.gene90760	0.845251	4.56E-59	-1.69E-46
MS.gene057915	MS.gene90760	0.824525	7.70E-54	-1.69E-46
MS.gene058010	MS.gene90760	0.844214	8.68E-59	-1.69E-46
MS.gene058146	MS.gene90760	0.820531	6.53E-53	-1.69E-46
MS.gene05847	MS.gene90760	0.813786	2.15E-51	-1.69E-46
MS.gene058548	MS.gene90760	0.822962	1.79E-53	-1.69E-46
MS.gene058595	MS.gene90760	0.824063	9.89E-54	-1.69E-46
MS.gene058625	MS.gene90760	0.812942	3.29E-51	-1.69E-46
MS.gene058979	MS.gene90760	0.825924	3.60E-54	-1.69E-46
MS.gene059161	MS.gene90760	0.824563	7.54E-54	-1.69E-46
MS.gene059725	MS.gene90760	0.813588	2.37E-51	-1.69E-46
MS.gene05979	MS.gene90760	0.807007	6.24E-50	-1.69E-46
MS.gene059842	MS.gene90760	0.836628	8.37E-57	-1.69E-46
MS.gene060011	MS.gene90760	0.819641	1.04E-52	-1.69E-46
MS.gene060167	MS.gene90760	0.828107	1.08E-54	-1.69E-46
MS.gene060310	MS.gene90760	0.845539	3.81E-59	-1.69E-46
MS.gene060312	MS.gene90760	0.81615	6.41E-52	-1.69E-46
MS.gene060322	MS.gene90760	0.841017	6.13E-58	-1.69E-46
MS.gene06052	MS.gene90760	0.814662	1.37E-51	-1.69E-46
MS.gene060683	MS.gene90760	0.817805	2.73E-52	-1.69E-46
MS.gene060685	MS.gene90760	0.821439	4.03E-53	-1.69E-46
MS.gene060802	MS.gene90760	0.847101	1.43E-59	-1.69E-46
MS.gene060803	MS.gene90760	0.850532	1.60E-60	-1.69E-46
MS.gene060998	MS.gene90760	0.85269	3.91E-61	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90760.t1	MTR_8g068320	100.000	66	0	1	66	1	66	4.84e-40	133
MS.gene90760.t1	MTR_8g068320	100.000	64	0	3	66	2	65	2.15e-38	129
MS.gene90760.t1	MTR_6g007797	48.980	49	25	15	63	7	55	4.56e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90760.t1	AT5G66240	67.797	59	19	7	65	3	61	6.12e-22	86.3
MS.gene90760.t1	AT5G66240	67.797	59	19	7	65	3	61	6.25e-22	86.3
MS.gene90760.t1	AT5G66240	67.797	59	19	7	65	6	64	6.31e-22	86.3

Find 8 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAGAGAAAGTTTCTT+TGG	0.262615	8.3:-34952652	MS.gene90760:CDS
GAATGGTAGAATTAGTTCTA+TGG	0.311519	8.3:-34952463	MS.gene90760:CDS
TGAAGAAATTCTTCTCAGTA+TGG	0.369274	8.3:-34952622	MS.gene90760:CDS
CGATTATACGATGTTGCTGC+TGG	0.402567	8.3:-34952387	MS.gene90760:CDS
ATTGGTTACGTAATCTTTGA+AGG	0.456413	8.3:+34952587	None:intergenic
ATTCTTCTCAGTATGGAAGT+TGG	0.458720	8.3:-34952615	MS.gene90760:CDS
AGCAACATCGTATAATCGAA+TGG	0.626818	8.3:+34952392	None:intergenic
AATTCATCTCCAACAATGGG+TGG	0.661924	8.3:-34952779	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAGAAAATAGTAGAAAT+TGG	+	chr8.3:34952582-34952601	None:intergenic	15.0%
!!!	TAATCAAATTCAACATTTTA+GGG	-	chr8.3:34952609-34952628	MS.gene90760:CDS	15.0%
!!!	TTAATCAAATTCAACATTTT+AGG	-	chr8.3:34952608-34952627	MS.gene90760:CDS	15.0%
!!	AAAACCAAGTAAAAAAGAAA+CGG	+	chr8.3:34952433-34952452	None:intergenic	20.0%
!	AAAAGAAGAGAAAGTTTCTT+TGG	-	chr8.3:34952496-34952515	MS.gene90760:intron	25.0%
!!!	TAGTTCTATGGATTTTCATA+GGG	-	chr8.3:34952697-34952716	MS.gene90760:intron	25.0%
!!!	TTAGTTCTATGGATTTTCAT+AGG	-	chr8.3:34952696-34952715	MS.gene90760:intron	25.0%
!!!	TTGTGCATTTTTTTACAGAA+TGG	-	chr8.3:34952668-34952687	MS.gene90760:CDS	25.0%
	ATTGGTTACGTAATCTTTGA+AGG	+	chr8.3:34952564-34952583	None:intergenic	30.0%
	TGAAGAAATTCTTCTCAGTA+TGG	-	chr8.3:34952526-34952545	MS.gene90760:intron	30.0%
!	AGGGTTTTTCATAGCATAAT+CGG	-	chr8.3:34952628-34952647	MS.gene90760:CDS	30.0%
!	ATTTTCGTGTTTTTCTTCTC+AGG	-	chr8.3:34952461-34952480	MS.gene90760:CDS	30.0%
!!	GAATGGTAGAATTAGTTCTA+TGG	-	chr8.3:34952685-34952704	MS.gene90760:intron	30.0%
	AGCAACATCGTATAATCGAA+TGG	+	chr8.3:34952759-34952778	None:intergenic	35.0%
	ATTCTTCTCAGTATGGAAGT+TGG	-	chr8.3:34952533-34952552	MS.gene90760:intron	35.0%
!	GGGTTTTTCATAGCATAATC+GGG	-	chr8.3:34952629-34952648	MS.gene90760:CDS	35.0%
!!	ACTTTCTCTTCTTTTTCACC+TGG	+	chr8.3:34952491-34952510	None:intergenic	35.0%
!!!	CGTTCCGTTTCTTTTTTACT+TGG	-	chr8.3:34952426-34952445	MS.gene90760:CDS	35.0%
!	TTTTTCTTCTCAGGAACACC+AGG	-	chr8.3:34952470-34952489	MS.gene90760:intron	40.0%
	CGATTATACGATGTTGCTGC+TGG	-	chr8.3:34952761-34952780	MS.gene90760:intron	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	34952384	34952786	34952384	ID=MS.gene90760
chr8.3	mRNA	34952384	34952786	34952384	ID=MS.gene90760.t1;Parent=MS.gene90760
chr8.3	exon	34952780	34952786	34952780	ID=MS.gene90760.t1.exon1;Parent=MS.gene90760.t1
chr8.3	CDS	34952780	34952786	34952780	ID=cds.MS.gene90760.t1;Parent=MS.gene90760.t1
chr8.3	exon	34952596	34952687	34952596	ID=MS.gene90760.t1.exon2;Parent=MS.gene90760.t1
chr8.3	CDS	34952596	34952687	34952596	ID=cds.MS.gene90760.t1;Parent=MS.gene90760.t1
chr8.3	exon	34952384	34952484	34952384	ID=MS.gene90760.t1.exon3;Parent=MS.gene90760.t1
chr8.3	CDS	34952384	34952484	34952384	ID=cds.MS.gene90760.t1;Parent=MS.gene90760.t1

Gene Sequence

>MS.gene90760

ATGGGTGGTACATCAAAATTATCACTACTGATTTTACACGCTCGTTCCGTTTCTTTTTTACTTGGTTTTTCGCTCATATTTTCGTGTTTTTCTTCTCAGGAACACCAGGTGAAAAAGAAGAGAAAGTTTCTTTGGATCTCAGTGAAGAAATTCTTCTCAGTATGGAAGTTGGAATGTCCTTCAAAGATTACGTAACCAATTTCTACTATTTTCTTTTTTGTTGTTTAATCAAATTCAACATTTTAGGGTTTTTCATAGCATAATCGGGTTTAATTATTTCTGTTTTGTGCATTTTTTTACAGAATGGTAGAATTAGTTCTATGGATTTTCATAGGGCATCAAGTTATCTTGTTACTGCTAGCGATGACGAATCCATTCGATTATACGATGTTGCTGCTGGAAC

Protein sequence

>MS.gene90760.t1

MGGTPGEKEEKVSLDLSEEILLSMEVGMSFKDYNGRISSMDFHRASSYLVTASDDESIRLYDVAAG