Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051316.t1 | AFK49349.1 | 95.3 | 86 | 4 | 0 | 2 | 87 | 106 | 191 | 1.30E-40 | 175.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051316.t1 | G7KCE8 | 95.3 | 86 | 4 | 0 | 2 | 87 | 106 | 191 | 9.2e-41 | 175.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051316 | MS.gene072397 | 0.805517 | 1.28E-49 | -1.69E-46 |
| MS.gene051316 | MS.gene21710 | 0.817229 | 3.67E-52 | -1.69E-46 |
| MS.gene051316 | MS.gene21912 | 0.825553 | 4.40E-54 | -1.69E-46 |
| MS.gene051316 | MS.gene28209 | -0.800987 | 1.11E-48 | -1.69E-46 |
| MS.gene051316 | MS.gene35437 | 0.801671 | 8.07E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051316.t1 | MTR_5g029600 | 95.349 | 86 | 4 | 0 | 2 | 87 | 106 | 191 | 2.86e-57 | 174 |
| MS.gene051316.t1 | MTR_8g066470 | 50.588 | 85 | 37 | 1 | 8 | 87 | 123 | 207 | 5.20e-25 | 92.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAAGACTCCCGGTCATTC+TGG | 0.203594 | 5.4:-19922570 | MS.gene051316:CDS |
| ATGGTAGTCCCAGCAGGATT+TGG | 0.379021 | 5.4:+19922468 | None:intergenic |
| GACAAAATTACAGAGTGAAT+TGG | 0.381429 | 5.4:-19922404 | MS.gene051316:CDS |
| AGCCTTTCCCTAGCATTTCA+AGG | 0.404469 | 5.4:-19922503 | MS.gene051316:CDS |
| ATTGGTCAGGGAGTGTTATC+AGG | 0.414663 | 5.4:-19922386 | MS.gene051316:CDS |
| AATTACAGAGTGAATTGGTC+AGG | 0.416251 | 5.4:-19922399 | MS.gene051316:CDS |
| ACAAGACTCCCGGTCATTCT+GGG | 0.445578 | 5.4:-19922569 | MS.gene051316:CDS |
| ACCATGACAAAGCATGCAAC+TGG | 0.472963 | 5.4:-19922603 | MS.gene051316:CDS |
| CTTGAATAGCCCAGAATGAC+CGG | 0.475683 | 5.4:+19922560 | None:intergenic |
| TATTCAAGAAGGACTCTGAT+AGG | 0.500114 | 5.4:-19922545 | MS.gene051316:CDS |
| TCTGAATGTCTCAGGGGTGT+AGG | 0.500295 | 5.4:+19922425 | None:intergenic |
| CCATGGATGGTAGTCCCAGC+AGG | 0.504947 | 5.4:+19922462 | None:intergenic |
| ATGATACCACCAAATCCTGC+TGG | 0.505763 | 5.4:-19922477 | MS.gene051316:CDS |
| TCTCAGGGGTGTAGGTACTA+AGG | 0.531544 | 5.4:+19922433 | None:intergenic |
| ATCATCATCCTTGAAATGCT+AGG | 0.531991 | 5.4:+19922495 | None:intergenic |
| GTAGTCCCAGCAGGATTTGG+TGG | 0.545146 | 5.4:+19922471 | None:intergenic |
| TCATCATCCTTGAAATGCTA+GGG | 0.554744 | 5.4:+19922496 | None:intergenic |
| GCCAGTTGCATGCTTTGTCA+TGG | 0.559486 | 5.4:+19922602 | None:intergenic |
| AGGTACTAAGGTTCCTGCCA+TGG | 0.565074 | 5.4:+19922445 | None:intergenic |
| ACTTCTAACCACAAGACTCC+CGG | 0.569693 | 5.4:-19922579 | MS.gene051316:CDS |
| CTGGGACTACCATCCATGGC+AGG | 0.576965 | 5.4:-19922458 | MS.gene051316:CDS |
| ATCCTTGAAATGCTAGGGAA+AGG | 0.577012 | 5.4:+19922501 | None:intergenic |
| CAGAATGACCGGGAGTCTTG+TGG | 0.579117 | 5.4:+19922571 | None:intergenic |
| ACTAAGGTTCCTGCCATGGA+TGG | 0.589882 | 5.4:+19922449 | None:intergenic |
| CCTGCTGGGACTACCATCCA+TGG | 0.594146 | 5.4:-19922462 | MS.gene051316:CDS |
| TTGAATAGCCCAGAATGACC+GGG | 0.602276 | 5.4:+19922561 | None:intergenic |
| TCATTCTGGGCTATTCAAGA+AGG | 0.603641 | 5.4:-19922556 | MS.gene051316:CDS |
| TGATACCACCAAATCCTGCT+GGG | 0.609975 | 5.4:-19922476 | MS.gene051316:CDS |
| ATTACAGAGTGAATTGGTCA+GGG | 0.619017 | 5.4:-19922398 | MS.gene051316:CDS |
| GTCAGGGAGTGTTATCAGGA+AGG | 0.623038 | 5.4:-19922382 | MS.gene051316:CDS |
| TCAGGAAGGACATCATGCAA+TGG | 0.644845 | 5.4:-19922368 | MS.gene051316:CDS |
| GCTAGGGAAAGGCTTTGATG+AGG | 0.660706 | 5.4:+19922512 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GACAAAATTACAGAGTGAAT+TGG | - | chr5.4:19922555-19922574 | MS.gene051316:CDS | 30.0% | |
| ! | AATTTTGTCTGAATGTCTCA+GGG | + | chr5.4:19922544-19922563 | None:intergenic | 30.0% |
| ! | TAATTTTGTCTGAATGTCTC+AGG | + | chr5.4:19922545-19922564 | None:intergenic | 30.0% |
| AATTACAGAGTGAATTGGTC+AGG | - | chr5.4:19922560-19922579 | MS.gene051316:CDS | 35.0% | |
| ATCATCATCCTTGAAATGCT+AGG | + | chr5.4:19922467-19922486 | None:intergenic | 35.0% | |
| ATTACAGAGTGAATTGGTCA+GGG | - | chr5.4:19922561-19922580 | MS.gene051316:CDS | 35.0% | |
| TATTCAAGAAGGACTCTGAT+AGG | - | chr5.4:19922414-19922433 | MS.gene051316:CDS | 35.0% | |
| TCATCATCCTTGAAATGCTA+GGG | + | chr5.4:19922466-19922485 | None:intergenic | 35.0% | |
| ! | ATTTTGTCTGAATGTCTCAG+GGG | + | chr5.4:19922543-19922562 | None:intergenic | 35.0% |
| ATCCTTGAAATGCTAGGGAA+AGG | + | chr5.4:19922461-19922480 | None:intergenic | 40.0% | |
| TCATTCTGGGCTATTCAAGA+AGG | - | chr5.4:19922403-19922422 | MS.gene051316:CDS | 40.0% | |
| ACTTCTAACCACAAGACTCC+CGG | - | chr5.4:19922380-19922399 | MS.gene051316:CDS | 45.0% | |
| AGCCTTTCCCTAGCATTTCA+AGG | - | chr5.4:19922456-19922475 | MS.gene051316:CDS | 45.0% | |
| ATGATACCACCAAATCCTGC+TGG | - | chr5.4:19922482-19922501 | MS.gene051316:CDS | 45.0% | |
| CTTGAATAGCCCAGAATGAC+CGG | + | chr5.4:19922402-19922421 | None:intergenic | 45.0% | |
| TCAGGAAGGACATCATGCAA+TGG | - | chr5.4:19922591-19922610 | MS.gene051316:CDS | 45.0% | |
| TGATACCACCAAATCCTGCT+GGG | - | chr5.4:19922483-19922502 | MS.gene051316:CDS | 45.0% | |
| TTGAATAGCCCAGAATGACC+GGG | + | chr5.4:19922401-19922420 | None:intergenic | 45.0% | |
| !! | ATTGGTCAGGGAGTGTTATC+AGG | - | chr5.4:19922573-19922592 | MS.gene051316:CDS | 45.0% |
| ACAAGACTCCCGGTCATTCT+GGG | - | chr5.4:19922390-19922409 | MS.gene051316:CDS | 50.0% | |
| ACTAAGGTTCCTGCCATGGA+TGG | + | chr5.4:19922513-19922532 | None:intergenic | 50.0% | |
| AGGTACTAAGGTTCCTGCCA+TGG | + | chr5.4:19922517-19922536 | None:intergenic | 50.0% | |
| ATGGTAGTCCCAGCAGGATT+TGG | + | chr5.4:19922494-19922513 | None:intergenic | 50.0% | |
| ! | GCTAGGGAAAGGCTTTGATG+AGG | + | chr5.4:19922450-19922469 | None:intergenic | 50.0% |
| ! | TCTCAGGGGTGTAGGTACTA+AGG | + | chr5.4:19922529-19922548 | None:intergenic | 50.0% |
| ! | TCTGAATGTCTCAGGGGTGT+AGG | + | chr5.4:19922537-19922556 | None:intergenic | 50.0% |
| !! | GTCAGGGAGTGTTATCAGGA+AGG | - | chr5.4:19922577-19922596 | MS.gene051316:CDS | 50.0% |
| CACAAGACTCCCGGTCATTC+TGG | - | chr5.4:19922389-19922408 | MS.gene051316:CDS | 55.0% | |
| CAGAATGACCGGGAGTCTTG+TGG | + | chr5.4:19922391-19922410 | None:intergenic | 55.0% | |
| GTAGTCCCAGCAGGATTTGG+TGG | + | chr5.4:19922491-19922510 | None:intergenic | 55.0% | |
| CCATGGATGGTAGTCCCAGC+AGG | + | chr5.4:19922500-19922519 | None:intergenic | 60.0% | |
| CCTGCTGGGACTACCATCCA+TGG | - | chr5.4:19922497-19922516 | MS.gene051316:CDS | 60.0% | |
| CTGGGACTACCATCCATGGC+AGG | - | chr5.4:19922501-19922520 | MS.gene051316:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 19922359 | 19922622 | 19922359 | ID=MS.gene051316 |
| chr5.4 | mRNA | 19922359 | 19922622 | 19922359 | ID=MS.gene051316.t1;Parent=MS.gene051316 |
| chr5.4 | exon | 19922359 | 19922622 | 19922359 | ID=MS.gene051316.t1.exon1;Parent=MS.gene051316.t1 |
| chr5.4 | CDS | 19922359 | 19922622 | 19922359 | ID=cds.MS.gene051316.t1;Parent=MS.gene051316.t1 |
| Gene Sequence |
| Protein sequence |
