AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052708

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052708.t1 MTR_8g018650 74.074 837 183 7 1 833 1 807 0.0 1268
MS.gene052708.t1 MTR_8g018620 73.150 838 189 9 1 833 1 807 0.0 1243
MS.gene052708.t1 MTR_8g018570 65.000 880 251 9 1 833 1 870 0.0 1159
MS.gene052708.t1 MTR_8g018550 65.718 878 246 7 1 833 1 868 0.0 1148
MS.gene052708.t1 MTR_8g020990 64.579 878 256 7 1 833 1 868 0.0 1142
MS.gene052708.t1 MTR_8g018690 63.823 879 258 7 1 833 1 865 0.0 1135
MS.gene052708.t1 MTR_8g018430 63.176 869 269 7 6 833 3 861 0.0 1127
MS.gene052708.t1 MTR_8g018520 63.212 878 268 7 1 833 1 868 0.0 1099
MS.gene052708.t1 MTR_8g018510 61.775 879 280 9 1 833 1 869 0.0 1094
MS.gene052708.t1 MTR_8g018690 61.889 879 255 8 1 833 1 845 0.0 1090
MS.gene052708.t1 MTR_8g018450 61.348 846 287 8 7 833 4 828 0.0 1060
MS.gene052708.t1 MTR_2g099570 58.783 871 311 9 1 833 1 861 0.0 1023
MS.gene052708.t1 MTR_8g018420 56.943 857 321 8 15 833 10 856 0.0 993
MS.gene052708.t1 MTR_8g018590 58.617 853 284 13 10 833 11 823 0.0 972
MS.gene052708.t1 MTR_5g024020 55.734 872 326 12 10 833 8 867 0.0 962
MS.gene052708.t1 MTR_2g099560 55.747 870 331 9 6 833 6 863 0.0 952
MS.gene052708.t1 MTR_8g018730 54.983 873 334 11 8 833 4 864 0.0 943
MS.gene052708.t1 MTR_8g018735 54.191 871 340 11 10 833 7 865 0.0 937
MS.gene052708.t1 MTR_8g018730 55.201 846 344 11 8 833 4 834 0.0 928
MS.gene052708.t1 MTR_3g081130 52.650 868 356 10 8 833 4 858 0.0 914
MS.gene052708.t1 MTR_5g024020 53.899 872 306 12 10 833 8 831 0.0 910
MS.gene052708.t1 MTR_3g081130 55.013 798 316 9 66 833 2 786 0.0 894
MS.gene052708.t1 MTR_7g113410 51.455 859 362 12 15 833 24 867 0.0 861
MS.gene052708.t1 MTR_1g104650 49.885 868 378 11 9 833 3 856 0.0 854
MS.gene052708.t1 MTR_1g083020 51.491 872 361 12 7 833 4 858 0.0 854
MS.gene052708.t1 MTR_8g018730 58.219 584 212 6 279 833 4 584 0.0 685
MS.gene052708.t1 MTR_8g020970 44.501 782 390 11 88 832 152 926 0.0 655
MS.gene052708.t1 MTR_8g021020 74.505 404 99 1 434 833 28 431 0.0 637
MS.gene052708.t1 MTR_4g066170 42.895 760 393 14 109 833 153 906 0.0 607
MS.gene052708.t1 MTR_8g018735 51.129 620 245 10 10 582 7 615 0.0 607
MS.gene052708.t1 MTR_3g479460 43.101 761 387 14 111 833 151 903 0.0 595
MS.gene052708.t1 MTR_8g011340 40.050 804 416 16 38 796 48 830 0.0 587
MS.gene052708.t1 MTR_3g079450 42.050 761 395 13 111 833 150 902 0.0 577
MS.gene052708.t1 MTR_8g080230 41.809 763 396 14 111 833 162 916 0.0 574
MS.gene052708.t1 MTR_4g088810 38.682 804 444 13 51 830 114 892 0.0 565
MS.gene052708.t1 MTR_3g079420 41.699 765 399 14 108 833 109 865 0.0 546
MS.gene052708.t1 MTR_6g452500 39.737 609 311 9 249 816 12 605 3.53e-148 449
MS.gene052708.t1 MTR_8g020970 43.421 532 257 11 88 582 152 676 1.92e-128 400
MS.gene052708.t1 MTR_3g079450 41.142 508 256 11 111 582 150 650 1.13e-112 358
MS.gene052708.t1 MTR_7g012030 60.504 119 46 1 485 602 85 203 1.38e-40 149
MS.gene052708.t1 MTR_6g046330 54.615 130 47 2 485 602 75 204 2.30e-35 137
MS.gene052708.t1 MTR_8g463810 32.661 248 95 6 372 618 181 357 1.85e-30 125
MS.gene052708.t1 MTR_3g107560 30.088 226 98 7 610 833 7 174 2.03e-19 87.0
MS.gene052708.t1 MTR_4g088805 38.462 91 56 0 742 832 1 91 2.42e-13 67.0
MS.gene052708.t1 MTR_2g005920 51.786 56 27 0 706 761 58 113 5.21e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052708.t1 AT1G55020 50.346 866 368 14 10 833 14 859 0.0 852
MS.gene052708.t1 AT3G22400 49.658 876 374 15 9 833 27 886 0.0 843
MS.gene052708.t1 AT1G17420 44.518 757 380 11 111 833 169 919 0.0 648
MS.gene052708.t1 AT1G72520 44.079 760 380 11 111 833 175 926 0.0 639
MS.gene052708.t1 AT3G45140 42.970 761 389 18 109 833 145 896 0.0 580
MS.gene052708.t1 AT3G45140 42.970 761 389 18 109 833 3 754 0.0 579
MS.gene052708.t1 AT1G67560 42.243 767 388 15 110 833 163 917 0.0 578

Find 192 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCATGCAGCTATTAATTT+TGG 0.141947 8.1:+79583580 MS.gene052708:CDS
TCTCCCAATGTTGGTATTAA+AGG 0.150987 8.1:-79578986 None:intergenic
GCATTTATTCTTCTCAAATA+TGG 0.209874 8.1:-79580736 None:intergenic
GGCTTGTATCGTCTAATTCT+TGG 0.214153 8.1:+79579687 MS.gene052708:CDS
CTTCTGATGTTTACAAGAAT+TGG 0.215197 8.1:+79581283 MS.gene052708:CDS
AATAAATGCAACTGGCTTAA+AGG 0.223947 8.1:+79580750 MS.gene052708:CDS
TACGATGTCTACAATGATTT+AGG 0.237255 8.1:+79579645 MS.gene052708:CDS
AGGATTGATATCCAACTTTA+TGG 0.249177 8.1:+79580468 MS.gene052708:CDS
GACGATACAAGCCAGGATTT+TGG 0.280553 8.1:-79579677 None:intergenic
CATCCTGATGGTGATAGTTT+TGG 0.299418 8.1:+79580850 MS.gene052708:CDS
CTTCTGATGGACTAGAGATA+TGG 0.305363 8.1:+79583347 MS.gene052708:CDS
CATTATGAACATGACCATTA+TGG 0.306288 8.1:+79583747 MS.gene052708:CDS
ATTCCGAGCTCCAAGCATTC+TGG 0.322998 8.1:+79583440 MS.gene052708:CDS
GCTTGTATCGTCTAATTCTT+GGG 0.326343 8.1:+79579688 MS.gene052708:CDS
CCAACTTACTTCCAAACTTC+TGG 0.344499 8.1:-79583864 None:intergenic
GCTTCAACACCTTGACTTGC+TGG 0.346948 8.1:-79580895 None:intergenic
TGGAGACAAATATCATCAAT+AGG 0.355090 8.1:+79576967 MS.gene052708:CDS
GGAGACGAAGGTTCTGCTTT+CGG 0.365441 8.1:+79579034 MS.gene052708:CDS
TAAGCATAAGATGACTTCAT+TGG 0.368930 8.1:+79576947 None:intergenic
CTTGGGTTGCAACATCTTCA+AGG 0.374201 8.1:-79579153 None:intergenic
GAAGAGTTGAAGACTTTAAG+AGG 0.376977 8.1:+79579579 MS.gene052708:CDS
TTCTGATGTTTACAAGAATT+GGG 0.377935 8.1:+79581284 MS.gene052708:CDS
CATTCATCATAGCAACAAAT+AGG 0.380660 8.1:+79581109 MS.gene052708:CDS
CAGTTGGCCTACCAGAAGTT+TGG 0.387223 8.1:+79583853 MS.gene052708:CDS
GTTCTTTATTTGCAAAATGA+TGG 0.388748 8.1:+79580790 MS.gene052708:CDS
TCCCAGCACATGGGAATTCC+AGG 0.390636 8.1:+79579076 MS.gene052708:CDS
ATAGAGTCAACATACTTGTT+TGG 0.392246 8.1:+79581240 MS.gene052708:CDS
GGAAGTAAAAGTTTATGAAT+AGG 0.397518 8.1:-79581150 None:intergenic
CATAATGGTCATGTTCATAA+TGG 0.404031 8.1:-79583746 None:intergenic
CAAGTTCTTTCCAGAATGCT+TGG 0.406750 8.1:-79583450 None:intergenic
AAGGTGTTGAAGCTTCAATT+TGG 0.410740 8.1:+79580905 MS.gene052708:CDS
CTCCTGGAATTCCCATGTGC+TGG 0.411294 8.1:-79579078 None:intergenic
TTGAGAAGAATAAATGCAAC+TGG 0.415813 8.1:+79580742 MS.gene052708:CDS
CTTGTTTGGAAGTTATTCAA+TGG 0.417879 8.1:+79581254 MS.gene052708:CDS
TGGCCAAGTAATTCATCTCT+TGG 0.419645 8.1:-79579969 None:intergenic
CTTTATCCTTCAAGTGATCC+AGG 0.419657 8.1:+79583973 MS.gene052708:CDS
GAAGTAAAAGTTTATGAATA+GGG 0.419737 8.1:-79581149 None:intergenic
GCTGCATCACCAACAACATT+GGG 0.420787 8.1:-79577041 None:intergenic
CTATTTGTTGCTATGATGAA+TGG 0.420998 8.1:-79581108 None:intergenic
TACTCAGGGGTTCCTATCTC+AGG 0.423400 8.1:-79583661 None:intergenic
TTCTCAACATAAACAGCTCC+TGG 0.423722 8.1:-79579094 None:intergenic
TTCTAGATGGAGATACTGTT+GGG 0.425448 8.1:-79584015 None:intergenic
ATCATTAGGTAGTCCTTGTT+CGG 0.425520 8.1:-79581313 None:intergenic
TCTTGTATGTAGTACACTAA+TGG 0.429054 8.1:-79579558 None:intergenic
GGCGTGTAAACATTACTAAC+AGG 0.434440 8.1:-79580874 None:intergenic
AACTGGAGAGCGCAAGGAAT+GGG 0.437269 8.1:+79579608 MS.gene052708:CDS
AGAAGATTCATGCCTGAGAT+AGG 0.443784 8.1:+79583649 MS.gene052708:CDS
CAATAGGGCCACAACTCTAA+AGG 0.444911 8.1:+79576983 MS.gene052708:CDS
AAACTTGTACCACTCTCATA+TGG 0.449448 8.1:+79583545 MS.gene052708:CDS
GTCAATCCTGGATCACTTGA+AGG 0.455620 8.1:-79583979 None:intergenic
AAAACACTTGGAACCATACA+TGG 0.456618 8.1:+79580515 MS.gene052708:CDS
TTTACAAGGGAGATGATTGC+TGG 0.457100 8.1:+79580298 MS.gene052708:CDS
ACGGATAATCCTTTATCAAA+AGG 0.457838 8.1:-79583321 None:intergenic
TACCCTCGAAGGTGTAGAAC+GGG 0.460074 8.1:+79579729 MS.gene052708:CDS
TCAACACCACTAGTGATTCA+AGG 0.460614 8.1:+79580146 MS.gene052708:CDS
TTCTGATGGACTAGAGATAT+GGG 0.461517 8.1:+79583348 MS.gene052708:CDS
TCAACAAGCTCCTCATACTC+AGG 0.462736 8.1:-79583676 None:intergenic
GAACCTTTAATACCAACATT+GGG 0.463993 8.1:+79578983 MS.gene052708:CDS
GGAACCTTTAATACCAACAT+TGG 0.464483 8.1:+79578982 MS.gene052708:CDS
ACTAGTGGTGTTGAAAACTT+TGG 0.466858 8.1:-79580137 None:intergenic
TTGAAGACTTTAAGAGGAGA+TGG 0.467418 8.1:+79579585 MS.gene052708:CDS
GGTCAGCCAGACCAAAATCC+TGG 0.468834 8.1:+79579666 MS.gene052708:CDS
TTTCTAGATGGAGATACTGT+TGG 0.468985 8.1:-79584016 None:intergenic
GGAGACAAATATCATCAATA+GGG 0.470362 8.1:+79576968 MS.gene052708:CDS
ATTCCAAGAGATGAATTACT+TGG 0.470803 8.1:+79579966 MS.gene052708:CDS
GGATGGGGTCAGTGTAGAAC+AGG 0.470907 8.1:+79580536 MS.gene052708:CDS
TCCTGGAATTCCCATGTGCT+GGG 0.476560 8.1:-79579077 None:intergenic
CATACCAATGGCTGACAAGT+TGG 0.480090 8.1:-79580972 None:intergenic
CTTCCCGTTCTACACCTTCG+AGG 0.480204 8.1:-79579732 None:intergenic
GTGTTAAACCCCAATGTTGT+TGG 0.480337 8.1:+79577032 MS.gene052708:CDS
GTTCTACACCTTCGAGGGTA+AGG 0.480400 8.1:-79579726 None:intergenic
ACTTTAAGAGGAGATGGAAC+TGG 0.480496 8.1:+79579591 MS.gene052708:CDS
AATAGGGCCACAACTCTAAA+GGG 0.483458 8.1:+79576984 MS.gene052708:CDS
GAAGGATAAAGCACAGTGTA+TGG 0.485574 8.1:-79583961 None:intergenic
CAAGCTGATTAGTGCTTCCA+TGG 0.486429 8.1:+79577118 MS.gene052708:CDS
AGGCGTTTCCACCGGAGAGC+AGG 0.487270 8.1:+79580448 MS.gene052708:intron
CTAGCTAGAGATCTTAATTG+AGG 0.494244 8.1:-79580050 None:intergenic
ATGCCTCTGAAAGTCAATCC+TGG 0.497159 8.1:-79583991 None:intergenic
TACCTAATGATCTAATCAAA+AGG 0.497300 8.1:+79581325 MS.gene052708:CDS
ACACTGACCCCATCCATGTA+TGG 0.497527 8.1:-79580528 None:intergenic
GGTTGACAATACCTCCATAA+TGG 0.501863 8.1:-79583761 None:intergenic
TTCAATTTGGAGCCTTGCCA+AGG 0.503212 8.1:+79580918 MS.gene052708:CDS
CACTTGGAACCATACATGGA+TGG 0.503494 8.1:+79580519 MS.gene052708:CDS
GATAGGAACCCCTGAGTATG+AGG 0.506154 8.1:+79583666 MS.gene052708:CDS
CCAGAAGTTTGGAAGTAAGT+TGG 0.506851 8.1:+79583864 MS.gene052708:CDS
ATGTGGAACACTTAGTTCAA+TGG 0.506969 8.1:-79580830 None:intergenic
GGAGAATCGAAGGTAGTCTC+TGG 0.510791 8.1:+79583812 MS.gene052708:CDS
AGTGGTGTTGAAAACTTTGG+AGG 0.512195 8.1:-79580134 None:intergenic
CCATTCCGACTCTCAATCCC+TGG 0.512454 8.1:-79579933 None:intergenic
TTTCCAGAATGCTTGGAGCT+CGG 0.513341 8.1:-79583443 None:intergenic
TATTCTGTCTAACAGGCTCT+CGG 0.513868 8.1:+79580670 MS.gene052708:intron
AGCTGCATCACCAACAACAT+TGG 0.517241 8.1:-79577042 None:intergenic
ACTTGGAACCATACATGGAT+GGG 0.517753 8.1:+79580520 MS.gene052708:CDS
CTGGAAAGAACTTGTAGAAG+TGG 0.520918 8.1:+79583459 MS.gene052708:CDS
TGAATGGGATTCCCAGCACA+TGG 0.525898 8.1:+79579066 MS.gene052708:CDS
ACAGTGTATGGCATCTTCAC+AGG 0.526792 8.1:-79583949 None:intergenic
CACAACTCCCTTTAGAGTTG+TGG 0.529501 8.1:-79576991 None:intergenic
GATTATCCGTATGCTTCTGA+TGG 0.530205 8.1:+79583334 MS.gene052708:CDS
TTCCCGTTCTACACCTTCGA+GGG 0.531782 8.1:-79579731 None:intergenic
GAACTGGAGAGCGCAAGGAA+TGG 0.534608 8.1:+79579607 MS.gene052708:CDS
AGTCACTGGAAAAGGAAAAG+TGG 0.535103 8.1:+79578943 MS.gene052708:intron
TTGGGTTGCAACATCTTCAA+GGG 0.535142 8.1:-79579152 None:intergenic
TGGTATTATCACCATAAAGT+TGG 0.536080 8.1:-79580479 None:intergenic
TGGATATCAATCCTGCTCTC+CGG 0.539625 8.1:-79580459 None:intergenic
GTCACTGGAAAAGGAAAAGT+GGG 0.541953 8.1:+79578944 MS.gene052708:intron
GATCCAGGATTGACTTTCAG+AGG 0.542922 8.1:+79583988 MS.gene052708:CDS
GGACCAAAACTATCACCATC+AGG 0.544353 8.1:-79580853 None:intergenic
ATTTACAACAACGTAAGCCT+TGG 0.544836 8.1:-79580935 None:intergenic
GAAATTATTACCATCAGCCA+TGG 0.548168 8.1:-79577135 None:intergenic
ATCACCAACTTGTCAGCCAT+TGG 0.550212 8.1:+79580968 MS.gene052708:CDS
TATGGGGTGCTATTAAGTCA+TGG 0.556682 8.1:+79583365 MS.gene052708:CDS
GAAGCTTTGTCTCCCAATGT+TGG 0.559602 8.1:-79578995 None:intergenic
CCAGGGATTGAGAGTCGGAA+TGG 0.560937 8.1:+79579933 MS.gene052708:CDS
AAGTGAGAGCACTTCATCAA+AGG 0.560945 8.1:-79580102 None:intergenic
CACAACTCTAAAGGGAGTTG+TGG 0.561678 8.1:+79576992 MS.gene052708:CDS
TTGCTTGTTATCTAGAGGAG+TGG 0.563970 8.1:+79583264 MS.gene052708:intron
CAACAAGCTCCTCATACTCA+GGG 0.566761 8.1:-79583675 None:intergenic
CAAGTTAGATGCAGCTGTTG+CGG 0.566896 8.1:+79583414 MS.gene052708:CDS
AGATGGAACTGGAGAGCGCA+AGG 0.567592 8.1:+79579602 MS.gene052708:CDS
ATGGGGTGCTATTAAGTCAT+GGG 0.569857 8.1:+79583366 MS.gene052708:CDS
GTCAACCCATGTTTCTGATG+AGG 0.571558 8.1:+79583777 MS.gene052708:CDS
CTTGTATCGTCTAATTCTTG+GGG 0.577400 8.1:+79579689 MS.gene052708:CDS
TGTATACCTCATCAGAAACA+TGG 0.579348 8.1:-79583783 None:intergenic
TTAACAATGTATCCACCGTA+TGG 0.580183 8.1:-79583610 None:intergenic
TTGGAACCTCACATCTCTCA+AGG 0.581017 8.1:-79579761 None:intergenic
TGACTGATGAGGAATTTACA+AGG 0.582002 8.1:+79580284 MS.gene052708:CDS
AACACAATGAACATAAATGC+CGG 0.582589 8.1:+79581183 MS.gene052708:CDS
TTATGATAGAGAGGTGCAGT+TGG 0.584724 8.1:+79583837 MS.gene052708:CDS
TGGAAAGAACTTGTAGAAGT+GGG 0.587691 8.1:+79583460 MS.gene052708:CDS
TTGAGCAAAAGCTCATTGAA+AGG 0.587750 8.1:+79583893 MS.gene052708:CDS
ACAGTATTGTCTAACAAGGT+AGG 0.589939 8.1:-79577089 None:intergenic
GTGTTAACAGCATAGTCTGA+AGG 0.591260 8.1:-79579999 None:intergenic
AGAATTAGACGATACAAGCC+AGG 0.591542 8.1:-79579684 None:intergenic
GAGTAGAGGATCGCACTGCA+TGG 0.591932 8.1:+79580260 MS.gene052708:intron
AATCAAGATATCTCGCGCAC+CGG 0.593820 8.1:-79581202 None:intergenic
TCATCAAAGGAGTTAAACTC+TGG 0.594584 8.1:-79580089 None:intergenic
GTCTAACAAGGTAGGAGGAA+CGG 0.595769 8.1:-79577081 None:intergenic
TATATATATGATCAGATCCA+GGG 0.595971 8.1:+79579916 MS.gene052708:intron
AAACTATCACCATCAGGATG+TGG 0.599290 8.1:-79580847 None:intergenic
CTGATGAGGTATACATCGGA+AGG 0.599510 8.1:+79583791 MS.gene052708:CDS
TCTAGTCCATCAGAAGCATA+CGG 0.600855 8.1:-79583340 None:intergenic
GTTTACACGCCAGCAAGTCA+AGG 0.601425 8.1:+79580886 MS.gene052708:CDS
TTACCCTCGAAGGTGTAGAA+CGG 0.602086 8.1:+79579728 MS.gene052708:CDS
TTGTACCACTCTCATATGGA+TGG 0.602656 8.1:+79583549 MS.gene052708:CDS
GCTTAAAGGCTTACGCCACA+AGG 0.605447 8.1:+79580764 MS.gene052708:CDS
ATTAGTGCTTCCATGGCTGA+TGG 0.606063 8.1:+79577125 MS.gene052708:CDS
TTCATTGTGTTACGATAGTG+TGG 0.606854 8.1:-79581171 None:intergenic
GCTGACAAGTTGGTGATATG+TGG 0.609612 8.1:-79580962 None:intergenic
CAACAACGTAAGCCTTGGCA+AGG 0.611036 8.1:-79580930 None:intergenic
CAAATAAAGAACGGTCCTTG+TGG 0.612677 8.1:-79580779 None:intergenic
CTTACTTCCAAACTTCTGGT+AGG 0.614464 8.1:-79583860 None:intergenic
CAGATCCAGGGATTGAGAGT+CGG 0.614572 8.1:+79579928 MS.gene052708:intron
AACAAATAGGCATCTTAGTG+TGG 0.616014 8.1:+79581122 MS.gene052708:CDS
TACATCGGAAGGAGAATCGA+AGG 0.617146 8.1:+79583802 MS.gene052708:CDS
ATATCAATCCTGCTCTCCGG+TGG 0.617544 8.1:-79580456 None:intergenic
GATATCTTGATTAACGCAGA+GGG 0.619675 8.1:+79581213 MS.gene052708:CDS
TCTCTGGACTTATGATAGAG+AGG 0.619857 8.1:+79583828 MS.gene052708:CDS
ATGTAGTACACTAATGGAGC+TGG 0.626012 8.1:-79579552 None:intergenic
GAAGACAATCACAACAATGA+CGG 0.626014 8.1:+79583720 MS.gene052708:CDS
GAATGGGATTCCCAGCACAT+GGG 0.629447 8.1:+79579067 MS.gene052708:CDS
GAACTTGTAGAAGTGGGTCA+TGG 0.630532 8.1:+79583466 MS.gene052708:CDS
GATATACCTTGAATCACTAG+TGG 0.631429 8.1:-79580152 None:intergenic
TCTAATGTAGGCGTTTCCAC+CGG 0.635207 8.1:+79580440 MS.gene052708:intron
CTTGGAACCATACATGGATG+GGG 0.635934 8.1:+79580521 MS.gene052708:CDS
ATACCACGCCTTACCCTCGA+AGG 0.638575 8.1:+79579718 MS.gene052708:CDS
ATCAAAAGGCGAACACCATG+TGG 0.640034 8.1:-79583307 None:intergenic
TCAAAGGAGTTAAACTCTGG+CGG 0.649224 8.1:-79580086 None:intergenic
GTATACCTCATCAGAAACAT+GGG 0.649932 8.1:-79583782 None:intergenic
TGAGTATGAGGAGCTTGTTG+AGG 0.650300 8.1:+79583678 MS.gene052708:CDS
TTTGGACAATATCCATACGG+TGG 0.651200 8.1:+79583598 MS.gene052708:CDS
TTGTATCGTCTAATTCTTGG+GGG 0.654005 8.1:+79579690 MS.gene052708:CDS
CATAGTCTGAAGGTTTCACA+TGG 0.654056 8.1:-79579989 None:intergenic
ACACTTAGTTCAATGGACAA+TGG 0.654958 8.1:-79580823 None:intergenic
GTTTCTGATGAGGTATACAT+CGG 0.656844 8.1:+79583787 MS.gene052708:CDS
AGATATCTTGATTAACGCAG+AGG 0.657092 8.1:+79581212 MS.gene052708:CDS
GTATTGTCTAACAAGGTAGG+AGG 0.658301 8.1:-79577086 None:intergenic
CTAAGTGTTCCACATCCTGA+TGG 0.659555 8.1:+79580838 MS.gene052708:CDS
GGTGATGCAGCTGATAATGA+AGG 0.660588 8.1:+79577053 MS.gene052708:CDS
GCTTGTTGAGGACTACGAGA+AGG 0.661543 8.1:+79583690 MS.gene052708:CDS
CTGCATCACCAACAACATTG+GGG 0.663667 8.1:-79577040 None:intergenic
AACGATACATCTTCTCCACA+TGG 0.664850 8.1:+79583292 MS.gene052708:CDS
TATGAACATGACCATTATGG+AGG 0.665625 8.1:+79583750 MS.gene052708:CDS
TGACACAGTATTGTCTAACA+AGG 0.669490 8.1:-79577093 None:intergenic
GACTGATGAGGAATTTACAA+GGG 0.671123 8.1:+79580285 MS.gene052708:CDS
TGAAGCCATCCATATGAGAG+TGG 0.678174 8.1:-79583554 None:intergenic
TCTGATGGACTAGAGATATG+GGG 0.686377 8.1:+79583349 MS.gene052708:CDS
CACTGCATGGATGACTGATG+AGG 0.693276 8.1:+79580273 MS.gene052708:CDS
AACAAGCTCCTCATACTCAG+GGG 0.722283 8.1:-79583674 None:intergenic
ACACCTTCGAGGGTAAGGCG+TGG 0.724628 8.1:-79579721 None:intergenic
ACAAGAAGACTGCAACATGG+TGG 0.739274 8.1:+79583491 MS.gene052708:CDS
GTGACAAGAAGACTGCAACA+TGG 0.743579 8.1:+79583488 MS.gene052708:CDS
AATATGGAAAGATTGCATCG+TGG 0.783955 8.1:-79580720 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr8.1 gene 79576957 79584035 79576957 ID=MS.gene052708
chr8.1 mRNA 79576957 79584035 79576957 ID=MS.gene052708.t1;Parent=MS.gene052708
chr8.1 exon 79576957 79577146 79576957 ID=MS.gene052708.t1.exon1;Parent=MS.gene052708.t1
chr8.1 CDS 79576957 79577146 79576957 ID=cds.MS.gene052708.t1;Parent=MS.gene052708.t1
chr8.1 exon 79578945 79579255 79578945 ID=MS.gene052708.t1.exon2;Parent=MS.gene052708.t1
chr8.1 CDS 79578945 79579255 79578945 ID=cds.MS.gene052708.t1;Parent=MS.gene052708.t1
chr8.1 exon 79579531 79579771 79579531 ID=MS.gene052708.t1.exon3;Parent=MS.gene052708.t1
chr8.1 CDS 79579531 79579771 79579531 ID=cds.MS.gene052708.t1;Parent=MS.gene052708.t1
chr8.1 exon 79579931 79580167 79579931 ID=MS.gene052708.t1.exon4;Parent=MS.gene052708.t1
chr8.1 CDS 79579931 79580167 79579931 ID=cds.MS.gene052708.t1;Parent=MS.gene052708.t1
chr8.1 exon 79580266 79580354 79580266 ID=MS.gene052708.t1.exon5;Parent=MS.gene052708.t1
chr8.1 CDS 79580266 79580354 79580266 ID=cds.MS.gene052708.t1;Parent=MS.gene052708.t1
chr8.1 exon 79580450 79580557 79580450 ID=MS.gene052708.t1.exon6;Parent=MS.gene052708.t1
chr8.1 CDS 79580450 79580557 79580450 ID=cds.MS.gene052708.t1;Parent=MS.gene052708.t1
chr8.1 exon 79580685 79580989 79580685 ID=MS.gene052708.t1.exon7;Parent=MS.gene052708.t1
chr8.1 CDS 79580685 79580989 79580685 ID=cds.MS.gene052708.t1;Parent=MS.gene052708.t1
chr8.1 exon 79581083 79581346 79581083 ID=MS.gene052708.t1.exon8;Parent=MS.gene052708.t1
chr8.1 CDS 79581083 79581346 79581083 ID=cds.MS.gene052708.t1;Parent=MS.gene052708.t1
chr8.1 exon 79583279 79584035 79583279 ID=MS.gene052708.t1.exon9;Parent=MS.gene052708.t1
chr8.1 CDS 79583279 79584035 79583279 ID=cds.MS.gene052708.t1;Parent=MS.gene052708.t1
Gene Sequence

>MS.gene052708

ATGACTTCATTGGAGACAAATATCATCAATAGGGCCACAACTCTAAAGGGAGTTGTGGTTTTGAAGCTAAAGAATGTGTTAAACCCCAATGTTGTTGGTGATGCAGCTGATAATGAAGGTGATACCGTTCCTCCTACCTTGTTAGACAATACTGTGTCATTCAAGCTGATTAGTGCTTCCATGGCTGATGGTAATAATTTCTTTATCTATAACTATACACTAACTTGCAAATAGACATTTTTTCTATAAAAATAGTGAAATTTATGCAACTGTTTTTAAAAAAAAATGCTAGTTACTTTCTCCATTTCTATTTATATATCATTTTAACAAAAAAAACAAAATTTGTTCATTGAAGAAGTAGCGTGTTTGAAGTTCGAACTTTGACTCTTGCATATGCAATTTCCTACCAATTGATTTAAACTCTCGGAAAATTTGTTTTACCAATGTTATTGAGAAATCATTTCATTCATTCATTTTATTTTCTCATATAAGGTGTACTTATAATGGATATTTATTCATTTTGCTAAATCAATTGCCTTTTTTTTTTTTCTTCAATAAACATCTTTGGAAACTACTCATAGCTCATTAATACATTCAGCTGTGTACGTAACATTTATTTATTTTGTTAAACCAATGAATATACCAATAAAGGCACTTTTGGAAGTAAAAAAAAAAATTGATTAAATTAGATTTCTTATTCCATGTGCATATGCTACAATGATATATATATTTCGGACGAAGGGAATATTTGATATAATAAATATTGATTAAATTGTAAATAAAATAATTTAAATACAAAATGACTCTAAAATTTGGTTAAAATCATTGATCAGATTAAAATGTCCTGAAAAATTATCTTAAAAATAAGTAATTAGACCAACATTTAAAATTTGTGAATATTTGTTTGATATTTTTTATTTCCAAGGACCAAAAGAAGTTTCAATATTTTCTTCTCAAGAACCAACTTGAGCTTTTATTTTGATGATGATTATTAATTATTATATGGTTGTCCATAAATATAGGAATGCATTGACTAAATCATTATAAGTAAAAAAATTGGTAGTAATATTCAAGCTTGTTGTTGACAATTGCTATTGTTTTACAAATTTATCTTTCATTAACATATGATTAATTTATATTTTTATCATATTTTTATCACATGTTCTAGAAAAAAGATGTAATATGAATAGCAACATGTTGGAAAATAAATAATTAAAAGAAGATTATAAAAATGGACAAGAAAAATCTCAAAATGATTCTATATTTATAGATGGAGGTAGTATATTTTTACGACCCCTTAGTTCAATTTCAAATAACGATTTAATTCTTTATTTTTTTCATGTCATTTTGGTCATTCACCCATGTATGAATATGAATTTCAAGTTTCGGATCTATGATTTTTTTTCCTTGTATGCTCTCAATCTTAAATACTCCATCCGGTCACTATTATAAGCAAAAATCAACTTTTTAGGTTCATTCATTAAATGATGTATTTGATCAATAATATAGACCAAATACATCATTTAATGAATGAACCTAAAAAGTTGATATTTGCTTATAATAGTGACCGGAGGGAGTATATGTTAAGATCCAACATAAATTCATAAATTTGAAACTTGAAAGTCCATATGTATAGATTATGGATCAAGAATCGTGCCTCTAATATCTTTCCTATCTAAATTTCATTTCACCATATATAATTGACCTTATGTTAAGGAGTAAGTTTCATACTCACTTCTTTCTTTCTAAAAAAAAAAGTTTCATACTCACTTTTATCTATGCAAGTAATTTTAGAAGAGTTTTTGACTAGGTCAGATATCAATTATCAGTTATCACTAAAATATCGAAAATATTTCACTTTGATACTTCATGCAACATGGATGACAAACTGTGTAGGCAAAAAGACAAATTGAGTTGTAATTAAATTTTTTATTTCTATTTGAGAATCATTAGTGAAATTGAGAAACTAATTTTTGACATTTTTATAGTCACTGGAAAAGGAAAAGTGGGAAAGCAAACGTTTTTGGAACCTTTAATACCAACATTGGGAGACAAAGCTTCTTCAGAAAAAACATTGGGAGACGAAGGTTCTGCTTTCGGTATTCATTTTGAATGGGATTCCCAGCACATGGGAATTCCAGGAGCTGTTTATGTTGAGAATTTTATGCAAGATGAATTCTTCCTTATCAAAATGTCCCTTGAAGATGTTGCAACCCAAGAAGCCATATTTTTTGTTTGCAATTCATGGGTTTACAATGCTGAAAAGTACAATACTGAACGCATTTTCTTTAGCAACAAGGTAAGGATATAAACTCTATCCATTTTTTTCATGCAACAAATGCTAAAAATTACTTCTTCGGGTCACTTTCATAAGCAAAAAACGGCTTTTTGGTTCGTTGAATAATTAATGCATCTAATCAATTATTCAACGAATCTATTTTTTTGTTTAAAATAGTAACTAGAGGGAGTATTAAAAATGTTCATAGAATTTTTTTTTATCATTTCCATGTAACTTTCTAAATTAGTATTCTCTTTTCCAAAAACTTGATACCTCCAATGGTTAAAATTATGTAGGCATACCTTCCAAGTAAAACACCAGCTCCATTAGTGTACTACATACAAGAAGAGTTGAAGACTTTAAGAGGAGATGGAACTGGAGAGCGCAAGGAATGGGAAAGAATATATGATTACGATGTCTACAATGATTTAGGTCAGCCAGACCAAAATCCTGGCTTGTATCGTCTAATTCTTGGGGGATCAAATACCACGCCTTACCCTCGAAGGTGTAGAACGGGAAGAAAACTCCTTGAGAGATGTGAGGTTCCAAACACTTGTGTCTGTTTGCAATATTTTTGCTGTGATATTTATTTTGGATTAAACATGTCTTTAAAGGCGCTATACCAGCTTGGTGCTATATTCTAAATGTTAACTCTGTTTGCAATTTTAAATATTTTGTTACTATATATATGATCAGATCCAGGGATTGAGAGTCGGAATGGATATTTTTACATTCCAAGAGATGAATTACTTGGCCATGTGAAACCTTCAGACTATGCTGTTAACACACTCAAATCAGCATCTGAATATGACACACCTCAATTAAGATCTCTAGCTAGAATACAAAACGATCCGCCAGAGTTTAACTCCTTTGATGAAGTGCTCTCACTTTTTGGCGAACCTCCAAAGTTTTCAACACCACTAGTGATTCAAGGTATATCTTAGTCAAAAATTTCAGAATTTCTAACAAATATATACAAAATCAAGTAAAGTAAGTTCATGGATCTTTTGAAAAATTCTTACTATGAGTAGAGGATCGCACTGCATGGATGACTGATGAGGAATTTACAAGGGAGATGATTGCTGGTGTGCATCCAAATGTCATCACAAAAATTTCTTCGGTAATTAAACTTAATAGTTAAATATTTTGAAGGAAACTTTTCATTCTACATTTATAATTGGTCATTAACCTTAGTGCTTGCTATATCTAATGTAGGCGTTTCCACCGGAGAGCAGGATTGATATCCAACTTTATGGTGATAATACCACCACAATAACCAAAAAACACTTGGAACCATACATGGATGGGGTCAGTGTAGAACAGGTAAACTGTTTCGTATTTCAACGTTACTCCAAAGACTTAAATGTTTTTATTATTATTTTTTCTGTTGAATTGATTTTTGTTTAAATTAAATGAACCACAAATGGCTAAATTTTATTCTGTCTAACAGGCTCTCGGAGACAATAGATTATACATACTTGATCACCACGATGCAATCTTTCCATATTTGAGAAGAATAAATGCAACTGGCTTAAAGGCTTACGCCACAAGGACCGTTCTTTATTTGCAAAATGATGGAACTTTGAAGCCATTGTCCATTGAACTAAGTGTTCCACATCCTGATGGTGATAGTTTTGGTCCTGTTAGTAATGTTTACACGCCAGCAAGTCAAGGTGTTGAAGCTTCAATTTGGAGCCTTGCCAAGGCTTACGTTGTTGTAAATGACGCCACATATCACCAACTTGTCAGCCATTGGTATGAATTTCATTATTATTTTTTTTTATACAATCAACTACTCTATTTGATCTATCTGAGTAATTTCATGAATTATATCTACATGTGTAATAGGTTGAACACTCATGCTTCTGTTGAGCCATTCATCATAGCAACAAATAGGCATCTTAGTGTGGTTCACCCTATTCATAAACTTTTACTTCCACACTATCGTAACACAATGAACATAAATGCCGGTGCGCGAGATATCTTGATTAACGCAGAGGGTATCATAGAGTCAACATACTTGTTTGGAAGTTATTCAATGGAATTGTCTTCTGATGTTTACAAGAATTGGGTTTTCACCGAACAAGGACTACCTAATGATCTAATCAAAAGGTAACCTTACAAAATATTACATCTTGGTCTAGTCCATTTAGAAAGACATTGATTTCATAAATAGTTTTTTATACTCATGAGATGTCAATCCATTGGTAAGAGTTGAACTATGGATCCTTAGAGGTAGTAGGGAATGAACAAAGCAACAACAACAACAACAACAACAACGCCTTATCCCACTAAGTGGGGTCGGCTACATGGATCAAATGCCGCCATAATGTTCTATCAAAGACCATATTTGGATCCAACTCATTGACCTCTAAATCTTTTCTAATGGTTTCTCTAATAGTTTTCTTAGGTCTTCCTCTACCTCTTTTAACCTGACTCTCCTCCATTTGATCTACTCTTCTTACCACGGCGTCTACGGGCCTTCTCTCTACATGTCCAAACCATCTAAGCCTATTTTCTACCAACTTTTCTACTATAGGTGCTACCCCCACTCTCTCTCTAATGATGTCATTCCTAATCCTATCATGTCTAGTCTTACCACTCATCCAACGCAACATCCTCATCTCCGCTACGCTTACTTTACTCTCGTGTTGGCTCTTAATCGCCCAACACTCCGTACCATACAACATTGCCGGTCTGACTGCTGTCCGATAGAACTTTCCTTTCAGCTTAAGTGGTACTTTCTTATCGCACAAAACACCTGAGGCTCTTCTCCATTTCAACCACCCAGCTTGAATACGATGATGTACATCAGCTTCTATTTCTCCGTCATTTTGTACTATGGACCCAAGATATCTAAACCGTGTAACTTGGGGTATGATATGATCTCCAACTTTCACCTCTAAGGTAGACCCACTTCTCCTTTTACTGAAGTTACATTCCATATATTCCGTCTTGTTTCTACTCAGGCGGAAACCGTACGCTTCTAAGGCTTGCCTCCAAGTCTCTAGCCTCCCGTTTAACTCCTCCCTCGACTCACCAAGCAAGACTACATCATCTGCAAAAAGCATACATCTCGGTGCCAACTCTTGGATGTGTTCCGTCAGTACATCCAAAACTAAAGTAAAAAGATAAGGACTTAGGGTTGACCCTTGGTGCAGTCCTATTGTTATGGGAAAATCTCCGGTATCCCCATCCTGCGTCCTCACACTAGTCGAAGCCCCCTCATACATATCCTGGATAGCTCTAATATAGGCAATCCTCACCCCTTTCTTCTCCAGGGCTTTCCACAAAATCTCTCTAGGTACTCTATCATACGCCTTCTCCAAATCAATGAAAACTAAGTGCAAGTCTTTTTTATCCGTCCGATATCGCTCCATCACGCGTCGAAGTAAGTAGATCGCTTCCATAGTCGACCTCCCAGGCATAAAACCAAATTGGTTATCCGTAACGTGAGTCTCTTTTCTTAGTCTTCGCTCAATTACCCTTTCCCATAACTTCATGGTATGACTCATTAGCTTAATTCCCCTATAATTCGCGCAATCTTGTATATCCCCCTTGTTCTTATAGATTGGAATTAAGGTGCTTCTTCTCCACTCGTCCGGCATTTTCTTCGTCCTCATAATCTCGTTGAAAAGTTTTGTGAGCCACCCAATTCCTCTATCGCCAAGACTTTTCCACACTTCAATAGGTATGTTGTCCGGCCCAACTGCCTTGCCATTACTCATCCTTTTCAACGCTTCTTTCACCTCGTGCTCCTGAATCCGGCGATAATAGTTATAGTTTCGGTCCTCCTCTCTGATGTCTAACCTGTTAGAGTCTGGTAAGATCTCATATCCTTCAATAGGGAATGAACAAAGCAACTACCAAATAAATAAGCTAACTATTTACTAACAAACTCTAGATAACTTTTCTAACTAGGTTTTATATTTCTAACTAGGTTTTATATTTGTAAAATAGTCATTATAATATCACTTAAATTAATAATAACATTTTTATAGTAATTTTTGTCTGTTAATGTTATCTTCCTTGCTTGTTATCTAGAGGAGTGGCTGTTAACGATACATCTTCTCCACATGGTGTTCGCCTTTTGATAAAGGATTATCCGTATGCTTCTGATGGACTAGAGATATGGGGTGCTATTAAGTCATGGGTTGAAGAATATGTAAATTTCTATTACAAGTTAGATGCAGCTGTTGCGGATGATTCCGAGCTCCAAGCATTCTGGAAAGAACTTGTAGAAGTGGGTCATGGTGACAAGAAGACTGCAACATGGTGGTTTAAGATGCAAAATCGTGCAGAGTTGATTGAAACTTGTACCACTCTCATATGGATGGCTTCAGCACTTCATGCAGCTATTAATTTTGGACAATATCCATACGGTGGATACATTGTTAACCGACCAACTAAAAGTAGAAGATTCATGCCTGAGATAGGAACCCCTGAGTATGAGGAGCTTGTTGAGGACTACGAGAAGGCGTATTTGAAGACAATCACAACAATGACGGATACCATTATGAACATGACCATTATGGAGGTATTGTCAACCCATGTTTCTGATGAGGTATACATCGGAAGGAGAATCGAAGGTAGTCTCTGGACTTATGATAGAGAGGTGCAGTTGGCCTACCAGAAGTTTGGAAGTAAGTTGGCTGAAATTGAGCAAAAGCTCATTGAAAGGAATAATGATGAGTCATTGAAAAACAGGAATGGGCCTGTGAAGATGCCATACACTGTGCTTTATCCTTCAAGTGATCCAGGATTGACTTTCAGAGGCATTCCCAACAGTATCTCCATCTAG
Protein sequence

>MS.gene052708.t1

MTSLETNIINRATTLKGVVVLKLKNVLNPNVVGDAADNEGDTVPPTLLDNTVSFKLISASMADVTGKGKVGKQTFLEPLIPTLGDKASSEKTLGDEGSAFGIHFEWDSQHMGIPGAVYVENFMQDEFFLIKMSLEDVATQEAIFFVCNSWVYNAEKYNTERIFFSNKAYLPSKTPAPLVYYIQEELKTLRGDGTGERKEWERIYDYDVYNDLGQPDQNPGLYRLILGGSNTTPYPRRCRTGRKLLERYPGIESRNGYFYIPRDELLGHVKPSDYAVNTLKSASEYDTPQLRSLARIQNDPPEFNSFDEVLSLFGEPPKFSTPLVIQEDRTAWMTDEEFTREMIAGVHPNVITKISSAFPPESRIDIQLYGDNTTTITKKHLEPYMDGVSVEQALGDNRLYILDHHDAIFPYLRRINATGLKAYATRTVLYLQNDGTLKPLSIELSVPHPDGDSFGPVSNVYTPASQGVEASIWSLAKAYVVVNDATYHQLVSHWLNTHASVEPFIIATNRHLSVVHPIHKLLLPHYRNTMNINAGARDILINAEGIIESTYLFGSYSMELSSDVYKNWVFTEQGLPNDLIKRGVAVNDTSSPHGVRLLIKDYPYASDGLEIWGAIKSWVEEYVNFYYKLDAAVADDSELQAFWKELVEVGHGDKKTATWWFKMQNRAELIETCTTLIWMASALHAAINFGQYPYGGYIVNRPTKSRRFMPEIGTPEYEELVEDYEKAYLKTITTMTDTIMNMTIMEVLSTHVSDEVYIGRRIEGSLWTYDREVQLAYQKFGSKLAEIEQKLIERNNDESLKNRNGPVKMPYTVLYPSSDPGLTFRGIPNSISI