AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59849


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59849.t1 MTR_8g045520 94.904 157 8 0 1 157 1 157 1.55e-109 308
MS.gene59849.t1 MTR_8g045490 81.529 157 28 1 1 157 1 156 1.94e-92 265
MS.gene59849.t1 MTR_8g045400 80.255 157 30 1 1 157 1 156 7.89e-91 261
MS.gene59849.t1 MTR_8g045695 79.618 157 31 1 1 157 1 156 9.00e-90 258
MS.gene59849.t1 MTR_8g045555 76.821 151 35 0 1 151 1 151 5.99e-87 251
MS.gene59849.t1 MTR_8g045300 75.641 156 38 0 1 156 1 156 1.96e-86 250
MS.gene59849.t1 MTR_8g045665 76.433 157 36 1 1 157 1 156 3.04e-85 247
MS.gene59849.t1 MTR_8g045570 76.159 151 36 0 1 151 1 151 3.90e-85 246
MS.gene59849.t1 MTR_8g045640 75.159 157 38 1 1 157 1 156 7.39e-84 243
MS.gene59849.t1 MTR_8g045735 74.194 155 39 1 1 155 1 154 1.98e-83 242
MS.gene59849.t1 MTR_8g045560 72.611 157 42 1 1 157 1 156 1.12e-80 235
MS.gene59849.t1 MTR_1g030810 42.208 154 86 2 1 152 1 153 4.02e-39 130
MS.gene59849.t1 MTR_1g030820 38.312 154 92 2 1 152 1 153 1.16e-37 126
MS.gene59849.t1 MTR_1g030840 36.538 156 91 4 1 149 1 155 5.89e-28 102
MS.gene59849.t1 MTR_8g012550 32.000 150 99 3 6 154 8 155 1.41e-25 95.9
MS.gene59849.t1 MTR_1g031640 35.374 147 86 5 7 151 7 146 4.32e-24 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59849.t1 AT5G28010 34.211 152 97 3 3 152 15 165 1.26e-26 99.0
MS.gene59849.t1 AT5G28010 34.211 152 97 3 3 152 15 165 1.26e-26 99.0
MS.gene59849.t1 AT1G70890 36.184 152 94 3 3 152 7 157 3.28e-26 97.4
MS.gene59849.t1 AT1G70840 32.895 152 99 3 3 152 20 170 2.95e-24 92.8
MS.gene59849.t1 AT1G70830 33.553 152 98 3 3 152 22 172 5.73e-24 92.0
MS.gene59849.t1 AT1G70830 33.553 152 98 3 3 152 22 172 1.07e-23 92.0
MS.gene59849.t1 AT1G23120 31.944 144 93 3 10 153 10 148 1.39e-23 90.5
MS.gene59849.t1 AT1G70870 37.748 151 81 4 1 151 1 138 1.43e-23 90.1
MS.gene59849.t1 AT1G70830 33.553 152 98 3 3 152 22 172 4.50e-23 91.7
MS.gene59849.t1 AT1G23120 31.944 144 93 3 10 153 60 198 5.23e-23 90.5
MS.gene59849.t1 AT1G70830 33.553 152 98 3 3 152 22 172 1.10e-22 92.0
MS.gene59849.t1 AT1G70830 32.237 152 100 3 3 152 184 334 3.56e-21 88.2
MS.gene59849.t1 AT5G28000 30.921 152 102 3 3 152 13 163 2.84e-22 87.4
MS.gene59849.t1 AT1G70850 32.450 151 99 3 3 151 9 158 4.89e-22 88.6
MS.gene59849.t1 AT1G70830 32.237 152 100 3 3 152 137 287 1.21e-21 88.6
MS.gene59849.t1 AT1G70830 28.421 95 67 1 59 152 31 125 2.96e-11 60.5
MS.gene59849.t1 AT1G70850 32.450 151 99 3 3 151 9 158 1.32e-21 89.0
MS.gene59849.t1 AT1G70850 32.394 142 93 3 13 152 175 315 7.17e-21 87.0
MS.gene59849.t1 AT1G70850 32.450 151 99 3 3 151 9 158 1.32e-21 89.0
MS.gene59849.t1 AT1G70850 32.394 142 93 3 13 152 175 315 7.17e-21 87.0
MS.gene59849.t1 AT1G24020 32.468 154 100 3 1 152 1 152 6.94e-19 78.6
MS.gene59849.t1 AT1G24020 32.468 154 100 3 1 152 1 152 6.94e-19 78.6
MS.gene59849.t1 AT4G23670 34.194 155 93 4 1 151 1 150 7.91e-19 78.2
MS.gene59849.t1 AT1G23130 27.152 151 108 2 3 152 10 159 2.06e-18 77.4
MS.gene59849.t1 AT1G70880 27.632 152 107 3 3 152 8 158 7.15e-18 75.9
MS.gene59849.t1 AT1G14930 29.605 152 104 3 1 151 1 150 3.27e-17 74.3
MS.gene59849.t1 AT1G35260 28.767 146 100 3 9 152 8 151 8.06e-17 73.2
MS.gene59849.t1 AT2G01520 28.947 152 105 3 1 151 1 150 5.41e-16 70.9
MS.gene59849.t1 AT4G23680 29.677 155 100 4 1 151 1 150 1.42e-15 69.7
MS.gene59849.t1 AT2G01530 28.947 152 105 3 1 151 1 150 1.79e-14 67.0
MS.gene59849.t1 AT4G14060 29.677 155 100 4 1 151 1 150 2.10e-14 66.6
MS.gene59849.t1 AT1G14940 28.477 151 105 3 1 150 1 149 1.36e-13 64.7
MS.gene59849.t1 AT1G14950 28.750 160 105 4 1 156 1 155 1.85e-13 64.3
MS.gene59849.t1 AT1G30990 29.801 151 102 4 1 150 1 148 3.95e-13 63.5
MS.gene59849.t1 AT3G26460 30.380 158 96 6 1 151 1 151 4.00e-11 58.2

Find 34 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGGTACTTACAAGCTCTT+TGG 0.306715 8.3:+53869126 MS.gene59849:CDS
ATGGCACTGCTGCTGCTAAA+TGG 0.310932 8.3:+53869211 MS.gene59849:CDS
AAGGTACTTACAAGCTCTTT+GGG 0.349654 8.3:+53869127 MS.gene59849:CDS
CACCCTTATCAATTACTTCA+AGG 0.382574 8.3:-53869188 None:intergenic
TATCCTTGAAGTAATTGATA+AGG 0.405951 8.3:+53869185 MS.gene59849:CDS
AGATATTGATGAGCACTATA+AGG 0.414096 8.3:+53869152 MS.gene59849:CDS
GGTAAACTTATTACTGAACT+TGG 0.415223 8.3:+53868259 MS.gene59849:CDS
ATCCTTGAAGTAATTGATAA+GGG 0.432462 8.3:+53869186 MS.gene59849:CDS
ATCTTCACCCTCATGCAGCT+TGG 0.445602 8.3:-53868368 None:intergenic
AAGTAGTCCATCCAGCCATT+TGG 0.456227 8.3:-53869273 None:intergenic
AAACACTGGACTTATGTCAT+AGG 0.470664 8.3:+53868412 MS.gene59849:CDS
TGAAATAACAACAGATGGTA+AGG 0.489687 8.3:+53869056 MS.gene59849:intron
AGCTGCATGAGGGTGAAGAT+TGG 0.493681 8.3:+53868371 MS.gene59849:CDS
TATGACTTATAGAGCAACCT+TGG 0.515175 8.3:-53869340 None:intergenic
TGATGCTCATCTTGTCAAGC+AGG 0.523708 8.3:+53869317 MS.gene59849:CDS
ATATTGACCCTCCAAATGGC+TGG 0.527845 8.3:+53869262 MS.gene59849:CDS
GGATCAAAACACCTGCTGAC+AGG 0.537625 8.3:+53868281 MS.gene59849:CDS
GAGGATATTGACCCTCCAAA+TGG 0.539495 8.3:+53869258 MS.gene59849:CDS
GTAAACTTATTACTGAACTT+GGG 0.542207 8.3:+53868260 MS.gene59849:CDS
AGATTGGCATCACACTGATT+CGG 0.545548 8.3:+53868387 MS.gene59849:CDS
ATTGATGAACAAAACAAGAA+AGG 0.549109 8.3:+53869108 MS.gene59849:CDS
GAAGTAATTGATAAGGGTGA+TGG 0.549119 8.3:+53869192 MS.gene59849:CDS
TAGTCCATCCAGCCATTTGG+AGG 0.551025 8.3:-53869270 None:intergenic
ACACTGATTCGGTCAAACAC+TGG 0.556043 8.3:+53868398 MS.gene59849:CDS
TGACCCTCCAAATGGCTGGA+TGG 0.557517 8.3:+53869266 MS.gene59849:CDS
AGTTTGAAGAACCTGTCAGC+AGG 0.558598 8.3:-53868292 None:intergenic
TCATCTTGTCAAGCAGGCCA+AGG 0.591246 8.3:+53869323 MS.gene59849:CDS
TCATGAAACCAAGCTGCATG+AGG 0.602712 8.3:+53868360 MS.gene59849:CDS
AGGTACTTACAAGCTCTTTG+GGG 0.621914 8.3:+53869128 MS.gene59849:CDS
CATGAAACCAAGCTGCATGA+GGG 0.630684 8.3:+53868361 MS.gene59849:CDS
GGTACTTACAAGCTCTTTGG+GGG 0.640678 8.3:+53869129 MS.gene59849:CDS
GGTACACACATGTCATGAGA+AGG 0.648648 8.3:+53869077 MS.gene59849:CDS
AGTCCATCCAGCCATTTGGA+GGG 0.649199 8.3:-53869269 None:intergenic
TGAATATGAGAAGATCAATG+AGG 0.699788 8.3:+53869239 MS.gene59849:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATATTTGTCTAATA+TGG + chr8.3:53868870-53868889 MS.gene59849:intron 10.0%
!! AATCTTACTAAAAAATGTTA+AGG - chr8.3:53868993-53869012 None:intergenic 15.0%
!! TAGACAAATATTATTATTGA+TGG - chr8.3:53868867-53868886 None:intergenic 15.0%
!!! TATTTTATTGAAAATAGCAA+CGG - chr8.3:53868584-53868603 None:intergenic 15.0%
!! AACAAAAAATTGTACAATAC+TGG - chr8.3:53868828-53868847 None:intergenic 20.0%
!! ACAAATATTATTATTGATGG+TGG - chr8.3:53868864-53868883 None:intergenic 20.0%
!!! GATCTCTAAAAAATGTTTAA+GGG + chr8.3:53868892-53868911 MS.gene59849:intron 20.0%
!!! TAAACTGCTGTTATTTTTTT+TGG + chr8.3:53869020-53869039 MS.gene59849:intron 20.0%
! AATTAAAGAGATGTACAGAA+AGG + chr8.3:53868962-53868981 MS.gene59849:intron 25.0%
! ATCCTTGAAGTAATTGATAA+GGG + chr8.3:53869186-53869205 MS.gene59849:CDS 25.0%
! ATTGATGAACAAAACAAGAA+AGG + chr8.3:53869108-53869127 MS.gene59849:CDS 25.0%
! GATCCTTATTAAAAATGGAA+AGG - chr8.3:53868806-53868825 None:intergenic 25.0%
! GTAAACTTATTACTGAACTT+GGG + chr8.3:53868260-53868279 MS.gene59849:CDS 25.0%
! TACTGGATCCTTATTAAAAA+TGG - chr8.3:53868811-53868830 None:intergenic 25.0%
! TATCCTTGAAGTAATTGATA+AGG + chr8.3:53869185-53869204 MS.gene59849:CDS 25.0%
! TGTTGTACTACAAATGATAA+CGG - chr8.3:53868922-53868941 None:intergenic 25.0%
!! GGATCTCTAAAAAATGTTTA+AGG + chr8.3:53868891-53868910 MS.gene59849:intron 25.0%
!!! GATCCTTTCCATTTTTAATA+AGG + chr8.3:53868800-53868819 MS.gene59849:intron 25.0%
AGATATTGATGAGCACTATA+AGG + chr8.3:53869152-53869171 MS.gene59849:CDS 30.0%
GGTAAACTTATTACTGAACT+TGG + chr8.3:53868259-53868278 MS.gene59849:CDS 30.0%
TGAAATAACAACAGATGGTA+AGG + chr8.3:53869056-53869075 MS.gene59849:intron 30.0%
TGAATATGAGAAGATCAATG+AGG + chr8.3:53869239-53869258 MS.gene59849:CDS 30.0%
TGTTGGCAAGTTAAATTAGT+TGG - chr8.3:53868449-53868468 None:intergenic 30.0%
!! ACCATCTGTTGTTATTTCAT+GGG - chr8.3:53869055-53869074 None:intergenic 30.0%
!! TACCATCTGTTGTTATTTCA+TGG - chr8.3:53869056-53869075 None:intergenic 30.0%
AAACACTGGACTTATGTCAT+AGG + chr8.3:53868412-53868431 MS.gene59849:CDS 35.0%
CAAAATCATCTTGTTGGACT+TGG + chr8.3:53868467-53868486 MS.gene59849:intron 35.0%
CACCCTTATCAATTACTTCA+AGG - chr8.3:53869191-53869210 None:intergenic 35.0%
GAAGTAATTGATAAGGGTGA+TGG + chr8.3:53869192-53869211 MS.gene59849:CDS 35.0%
TGCCAACAAAATCATCTTGT+TGG + chr8.3:53868461-53868480 MS.gene59849:intron 35.0%
! AAGGTACTTACAAGCTCTTT+GGG + chr8.3:53869127-53869146 MS.gene59849:CDS 35.0%
! GTCCAACAAGATGATTTTGT+TGG - chr8.3:53868466-53868485 None:intergenic 35.0%
!! AAAGGTACTTACAAGCTCTT+TGG + chr8.3:53869126-53869145 MS.gene59849:CDS 35.0%
AGGTACTTACAAGCTCTTTG+GGG + chr8.3:53869128-53869147 MS.gene59849:CDS 40.0%
GCCCATGAAATAACAACAGA+TGG + chr8.3:53869051-53869070 MS.gene59849:intron 40.0%
! AGATTGGCATCACACTGATT+CGG + chr8.3:53868387-53868406 MS.gene59849:CDS 40.0%
AAGTAGTCCATCCAGCCATT+TGG - chr8.3:53869276-53869295 None:intergenic 45.0%
AGTTTGAAGAACCTGTCAGC+AGG - chr8.3:53868295-53868314 None:intergenic 45.0%
ATATTGACCCTCCAAATGGC+TGG + chr8.3:53869262-53869281 MS.gene59849:CDS 45.0%
CATGAAACCAAGCTGCATGA+GGG + chr8.3:53868361-53868380 MS.gene59849:CDS 45.0%
GAGGATATTGACCCTCCAAA+TGG + chr8.3:53869258-53869277 MS.gene59849:CDS 45.0%
GGTACACACATGTCATGAGA+AGG + chr8.3:53869077-53869096 MS.gene59849:CDS 45.0%
GGTACTTACAAGCTCTTTGG+GGG + chr8.3:53869129-53869148 MS.gene59849:CDS 45.0%
TCATGAAACCAAGCTGCATG+AGG + chr8.3:53868360-53868379 MS.gene59849:CDS 45.0%
TGATGCTCATCTTGTCAAGC+AGG + chr8.3:53869317-53869336 MS.gene59849:CDS 45.0%
!! ACACTGATTCGGTCAAACAC+TGG + chr8.3:53868398-53868417 MS.gene59849:CDS 45.0%
AGCTGCATGAGGGTGAAGAT+TGG + chr8.3:53868371-53868390 MS.gene59849:CDS 50.0%
AGTCCATCCAGCCATTTGGA+GGG - chr8.3:53869272-53869291 None:intergenic 50.0%
ATCTTCACCCTCATGCAGCT+TGG - chr8.3:53868371-53868390 None:intergenic 50.0%
ATGGCACTGCTGCTGCTAAA+TGG + chr8.3:53869211-53869230 MS.gene59849:CDS 50.0%
GGATCAAAACACCTGCTGAC+AGG + chr8.3:53868281-53868300 MS.gene59849:CDS 50.0%
TAGTCCATCCAGCCATTTGG+AGG - chr8.3:53869273-53869292 None:intergenic 50.0%
TCATCTTGTCAAGCAGGCCA+AGG + chr8.3:53869323-53869342 MS.gene59849:CDS 50.0%
TGACCCTCCAAATGGCTGGA+TGG + chr8.3:53869266-53869285 MS.gene59849:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 53868247 53869356 53868247 ID=MS.gene59849
chr8.3 mRNA 53868247 53869356 53868247 ID=MS.gene59849.t1;Parent=MS.gene59849
chr8.3 exon 53868247 53868433 53868247 ID=MS.gene59849.t1.exon1;Parent=MS.gene59849.t1
chr8.3 CDS 53868247 53868433 53868247 ID=cds.MS.gene59849.t1;Parent=MS.gene59849.t1
chr8.3 exon 53869070 53869356 53869070 ID=MS.gene59849.t1.exon2;Parent=MS.gene59849.t1
chr8.3 CDS 53869070 53869356 53869070 ID=cds.MS.gene59849.t1;Parent=MS.gene59849.t1
Gene Sequence

>MS.gene59849

ATGGTGCTTGCTGGTAAACTTATTACTGAACTTGGGATCAAAACACCTGCTGACAGGTTCTTCAAACTATTTGCATCAGAACTTCATGAAGTGCAAAAACACTGTGAAAGAGTTCATGAAACCAAGCTGCATGAGGGTGAAGATTGGCATCACACTGATTCGGTCAAACACTGGACTTATGTCATAGGTAATTATATTTCCAACTAATTTAACTTGCCAACAAAATCATCTTGTTGGACTTGGATTTTTCTATCATTTAAGTTTATTTACGTGTTGATCACATTACTTAGTTTAGTTTATTATGTTTCATCACAAAAATAAAGCTTCAAGATAGCCGTTGCTATTTTCAATAAAATAGTAAGTTTAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATTTCAAAAAAATAAAAAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAAAAGATATGTAAAAAAGCAGATCCTTTCCATTTTTAATAAGGATCCAGTATTGTACAATTTTTTGTTGTTTATATTAGATCCACCATCAATAATAATATTTGTCTAATATGGATCTCTAAAAAATGTTTAAGGGTGAACCGTTATCATTTGTAGTACAACAATGAATTTAATAATTAAAAAAATTAAAGAGATGTACAGAAAGGTAGATCCTTAACATTTTTTAGTAAGATTGCACTCGTAAACTGCTGTTATTTTTTTTGGTATTAATTGCCCATGAAATAACAACAGATGGTAAGGTACACACATGTCATGAGAAGGTTGAAGAAATTGATGAACAAAACAAGAAAGGTACTTACAAGCTCTTTGGGGGAGATATTGATGAGCACTATAAGGACTTTAAGCTTATCCTTGAAGTAATTGATAAGGGTGATGGCACTGCTGCTGCTAAATGGACAGTTGAATATGAGAAGATCAATGAGGATATTGACCCTCCAAATGGCTGGATGGACTACTTATGCAAATGTACTCGAGATATTGATGCTCATCTTGTCAAGCAGGCCAAGGTTGCTCTATAA

Protein sequence

>MS.gene59849.t1

MVLAGKLITELGIKTPADRFFKLFASELHEVQKHCERVHETKLHEGEDWHHTDSVKHWTYVIDGKVHTCHEKVEEIDEQNKKGTYKLFGGDIDEHYKDFKLILEVIDKGDGTAAAKWTVEYEKINEDIDPPNGWMDYLCKCTRDIDAHLVKQAKVAL