Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052955.t1 | AES86666.1 | 100 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 1.50E-22 | 114.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052955.t1 | Q4SUE2 | 59.4 | 64 | 26 | 0 | 1 | 64 | 1 | 64 | 1.5e-10 | 66.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052955.t1 | G7JJD0 | 100.0 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 1.1e-22 | 114.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052955 | MS.gene073207 | 0.805502 | 1.29E-49 | -1.69E-46 |
| MS.gene052955 | MS.gene22252 | 0.811891 | 5.58E-51 | -1.69E-46 |
| MS.gene052955 | MS.gene24923 | 0.836374 | 9.72E-57 | -1.69E-46 |
| MS.gene052955 | MS.gene24933 | 0.802872 | 4.56E-49 | -1.69E-46 |
| MS.gene052955 | MS.gene25696 | 0.821527 | 3.85E-53 | -1.69E-46 |
| MS.gene052955 | MS.gene25697 | 0.809668 | 1.69E-50 | -1.69E-46 |
| MS.gene052955 | MS.gene96224 | -0.809448 | 1.88E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052955.t1 | MTR_4g012800 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 4.48e-37 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052955.t1 | AT1G15270 | 89.062 | 64 | 7 | 0 | 1 | 64 | 1 | 64 | 1.07e-33 | 109 |
| MS.gene052955.t1 | AT3G16040 | 84.375 | 64 | 10 | 0 | 1 | 64 | 1 | 64 | 3.63e-29 | 98.2 |
Find 16 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTCACAGAAGGGATCTTT+TGG | 0.287259 | 4.1:-83582806 | MS.gene052955:CDS |
| GGCTAACATCCAGAAGAAAA+AGG | 0.357013 | 4.1:-83582956 | MS.gene052955:CDS |
| TAATCCTTCTTATCAGACTT+TGG | 0.404695 | 4.1:+83584127 | None:intergenic |
| AGATTGAAGGTTACCTTGCT+TGG | 0.421229 | 4.1:+83584357 | None:intergenic |
| TCAGACTTTGGCTTCTTCAA+AGG | 0.486773 | 4.1:+83584139 | None:intergenic |
| GAAGCCAAAGTCTGATAAGA+AGG | 0.504619 | 4.1:-83584131 | MS.gene052955:CDS |
| ATGGATTGAACAGGCGCTAA+AGG | 0.513359 | 4.1:-83582843 | MS.gene052955:intron |
| GTTACCTTGCTTGGTCGACA+TGG | 0.525919 | 4.1:+83584366 | None:intergenic |
| GCTAAAGGAGCTGAAGGCTA+AGG | 0.540771 | 4.1:-83582828 | MS.gene052955:CDS |
| GGATCTGGACTCAAGAAAAG+TGG | 0.544869 | 4.1:-83582782 | MS.gene052955:CDS |
| GAAGGCTAAGGCCTCACAGA+AGG | 0.551780 | 4.1:-83582816 | MS.gene052955:CDS |
| AAAACCATGTCGACCAAGCA+AGG | 0.588270 | 4.1:-83584370 | None:intergenic |
| AAGGCTAAGGCCTCACAGAA+GGG | 0.624637 | 4.1:-83582815 | MS.gene052955:CDS |
| ACAGGCGCTAAAGGAGCTGA+AGG | 0.640092 | 4.1:-83582834 | MS.gene052955:intron |
| TCCAAAAGATCCCTTCTGTG+AGG | 0.701774 | 4.1:+83582805 | None:intergenic |
| TGATAAGAAGGATTACGATG+AGG | 0.705327 | 4.1:-83584119 | MS.gene052955:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTAATTTATTTTTTTTT+TGG | - | chr4.1:83583041-83583060 | MS.gene052955:intron | 0.0% |
| !!! | AATTTTTTGATTGATTTTTT+TGG | - | chr4.1:83582939-83582958 | MS.gene052955:intron | 10.0% |
| !! | ATAGTAATTATAAACAAAAG+AGG | - | chr4.1:83583267-83583286 | MS.gene052955:intron | 15.0% |
| !! | TAATAAAACCCAAAATTAAA+AGG | + | chr4.1:83583205-83583224 | None:intergenic | 15.0% |
| !!! | TTTTTTTTTGGATATTATGT+TGG | - | chr4.1:83583053-83583072 | MS.gene052955:intron | 15.0% |
| !! | ATAGAGTTATTAAAGATTGA+AGG | + | chr4.1:83582795-83582814 | None:intergenic | 20.0% |
| !! | CTAATCAAAGCTATTAATTT+GGG | + | chr4.1:83583515-83583534 | None:intergenic | 20.0% |
| !! | GAATTCTATTAATTCAAACA+TGG | + | chr4.1:83583457-83583476 | None:intergenic | 20.0% |
| !! | TCTAATCAAAGCTATTAATT+TGG | + | chr4.1:83583516-83583535 | None:intergenic | 20.0% |
| !!! | ACTCTAATTCCTTTTAATTT+TGG | - | chr4.1:83583193-83583212 | MS.gene052955:intron | 20.0% |
| !!! | AGAATTGAAATTTTCAAGTT+TGG | - | chr4.1:83583120-83583139 | MS.gene052955:intron | 20.0% |
| !!! | CTCTAATTCCTTTTAATTTT+GGG | - | chr4.1:83583194-83583213 | MS.gene052955:intron | 20.0% |
| !!! | TAATTGTATCTGTATTTTGT+AGG | - | chr4.1:83584150-83584169 | MS.gene052955:CDS | 20.0% |
| ! | ACAGATACAATTATATCACA+AGG | + | chr4.1:83584143-83584162 | None:intergenic | 25.0% |
| ! | ACTGTATATTTGAATTGTGT+TGG | - | chr4.1:83583093-83583112 | MS.gene052955:intron | 25.0% |
| ! | ATTGATATTCACACTGAATT+CGG | + | chr4.1:83583779-83583798 | None:intergenic | 25.0% |
| ! | TCATATTCTTCAATCCTTTA+AGG | - | chr4.1:83583805-83583824 | MS.gene052955:intron | 25.0% |
| ! | TGATAGAATATGTTCTACAA+TGG | + | chr4.1:83584021-83584040 | None:intergenic | 25.0% |
| ! | TTTCATATGTGTCTTCTTTA+AGG | - | chr4.1:83583350-83583369 | MS.gene052955:intron | 25.0% |
| !! | ACGAACAGATCTATATTTTA+GGG | + | chr4.1:83582904-83582923 | None:intergenic | 25.0% |
| !! | AGCTTTGATTAGAAAGTATA+AGG | - | chr4.1:83583523-83583542 | MS.gene052955:intron | 25.0% |
| !! | CTTTGATTAGAAAGTATAAG+GGG | - | chr4.1:83583525-83583544 | MS.gene052955:intron | 25.0% |
| !! | GCTTTGATTAGAAAGTATAA+GGG | - | chr4.1:83583524-83583543 | MS.gene052955:intron | 25.0% |
| !! | TTTTTGTTGCATTTTACTTG+AGG | - | chr4.1:83584105-83584124 | MS.gene052955:intron | 25.0% |
| !!! | CGAATTCTGTTTTTTTTCAA+CGG | + | chr4.1:83583415-83583434 | None:intergenic | 25.0% |
| !!! | GTTTGCTTTTTGTGATTTTT+AGG | - | chr4.1:83582961-83582980 | MS.gene052955:CDS | 25.0% |
| !!! | TATTGGGTGTTTTTGTTATA+GGG | - | chr4.1:83583170-83583189 | MS.gene052955:intron | 25.0% |
| !!! | TTATTGGGTGTTTTTGTTAT+AGG | - | chr4.1:83583169-83583188 | MS.gene052955:intron | 25.0% |
| AAGCAAAAGGATAGAAGAAA+TGG | + | chr4.1:83583755-83583774 | None:intergenic | 30.0% | |
| ACCATACCTAGAAATTAGAA+TGG | - | chr4.1:83583833-83583852 | MS.gene052955:intron | 30.0% | |
| CAACAGAATCAGGTAAAAAT+AGG | + | chr4.1:83584272-83584291 | None:intergenic | 30.0% | |
| CCATACCTAGAAATTAGAAT+GGG | - | chr4.1:83583834-83583853 | MS.gene052955:intron | 30.0% | |
| GGATATTAATGACAGACAAT+AGG | - | chr4.1:83583557-83583576 | MS.gene052955:intron | 30.0% | |
| GGTAAGAAGGAAATATACAT+AGG | - | chr4.1:83583609-83583628 | MS.gene052955:intron | 30.0% | |
| GTGGAATATTGTGTTAAGAA+TGG | - | chr4.1:83583890-83583909 | MS.gene052955:intron | 30.0% | |
| TAATCCTTCTTATCAGACTT+TGG | + | chr4.1:83583012-83583031 | None:intergenic | 30.0% | |
| TATGAAAAATCAGCAAACAG+TGG | + | chr4.1:83583337-83583356 | None:intergenic | 30.0% | |
| TTGAGATTTCTGCTGTTAAT+TGG | - | chr4.1:83584070-83584089 | MS.gene052955:intron | 30.0% | |
| ! | AAGGCTAATCTGAAATATGT+TGG | + | chr4.1:83583936-83583955 | None:intergenic | 30.0% |
| ! | CACGAACAGATCTATATTTT+AGG | + | chr4.1:83582905-83582924 | None:intergenic | 30.0% |
| !! | AAAGTATAAGGGGAGTATTT+GGG | - | chr4.1:83583535-83583554 | MS.gene052955:intron | 30.0% |
| !! | TGCTTTTTGTGATTTTTAGG+TGG | - | chr4.1:83582964-83582983 | MS.gene052955:CDS | 30.0% |
| !! | TTGAGCTTGGTTGTTTTATT+GGG | - | chr4.1:83583154-83583173 | MS.gene052955:intron | 30.0% |
| !! | TTTGAGCTTGGTTGTTTTAT+TGG | - | chr4.1:83583153-83583172 | MS.gene052955:intron | 30.0% |
| !!! | CATATTTCAGATTAGCCTTT+TGG | - | chr4.1:83583937-83583956 | MS.gene052955:intron | 30.0% |
| AAAAATCAGCAAACAGTGGT+AGG | + | chr4.1:83583333-83583352 | None:intergenic | 35.0% | |
| AACAGAATTAGGAGGTAAGA+AGG | - | chr4.1:83583596-83583615 | MS.gene052955:intron | 35.0% | |
| CAGGTTTGCATAACAGAATT+AGG | - | chr4.1:83583585-83583604 | MS.gene052955:intron | 35.0% | |
| GTTTGCATAACAGAATTAGG+AGG | - | chr4.1:83583588-83583607 | MS.gene052955:intron | 35.0% | |
| TGACAGACAATAGGATATTC+AGG | - | chr4.1:83583566-83583585 | MS.gene052955:intron | 35.0% | |
| TGATAAGAAGGATTACGATG+AGG | - | chr4.1:83583017-83583036 | MS.gene052955:intron | 35.0% | |
| TTGAAAGTCATGTCTCATGA+GGG | - | chr4.1:83583703-83583722 | MS.gene052955:intron | 35.0% | |
| TTTGAAAGTCATGTCTCATG+AGG | - | chr4.1:83583702-83583721 | MS.gene052955:intron | 35.0% | |
| ! | CCCATTCTAATTTCTAGGTA+TGG | + | chr4.1:83583837-83583856 | None:intergenic | 35.0% |
| ! | TTTTACCTGATTCTGTTGCA+TGG | - | chr4.1:83584274-83584293 | MS.gene052955:intron | 35.0% |
| !! | AAGTATAAGGGGAGTATTTG+GGG | - | chr4.1:83583536-83583555 | MS.gene052955:intron | 35.0% |
| !! | CTGTATTTTGTAGGTTGACA+TGG | - | chr4.1:83584159-83584178 | MS.gene052955:intron | 35.0% |
| !! | GAAAGTATAAGGGGAGTATT+TGG | - | chr4.1:83583534-83583553 | MS.gene052955:intron | 35.0% |
| AGATTGAAGGTTACCTTGCT+TGG | + | chr4.1:83582782-83582801 | None:intergenic | 40.0% | |
| AGGTATGGTAGTTGCCTTAA+AGG | + | chr4.1:83583822-83583841 | None:intergenic | 40.0% | |
| ATGTAGAGTAGAGAGTCTAG+AGG | - | chr4.1:83583668-83583687 | MS.gene052955:intron | 40.0% | |
| CACTGAATTCGGTAAGCAAA+AGG | + | chr4.1:83583768-83583787 | None:intergenic | 40.0% | |
| CATCCAGAAGAAAAAGGATG+AGG | - | chr4.1:83584186-83584205 | MS.gene052955:intron | 40.0% | |
| CCACTCCCATTCTAATTTCT+AGG | + | chr4.1:83583842-83583861 | None:intergenic | 40.0% | |
| CCTAGAAATTAGAATGGGAG+TGG | - | chr4.1:83583839-83583858 | MS.gene052955:intron | 40.0% | |
| GAAGAAAAAGGATGAGGAGA+AGG | - | chr4.1:83584192-83584211 | MS.gene052955:intron | 40.0% | |
| GAAGCCAAAGTCTGATAAGA+AGG | - | chr4.1:83583005-83583024 | MS.gene052955:intron | 40.0% | |
| GGCTAACATCCAGAAGAAAA+AGG | - | chr4.1:83584180-83584199 | MS.gene052955:intron | 40.0% | |
| GTAAGGGTAGGAAGATTTCA+GGG | - | chr4.1:83583994-83584013 | MS.gene052955:intron | 40.0% | |
| GTCATTCATTTGGTAAGGGT+AGG | - | chr4.1:83583982-83584001 | MS.gene052955:intron | 40.0% | |
| GTCGGTCATTCATTTGGTAA+GGG | - | chr4.1:83583978-83583997 | MS.gene052955:intron | 40.0% | |
| TCAATCCATGCAACAGAATC+AGG | + | chr4.1:83584282-83584301 | None:intergenic | 40.0% | |
| TGTAGAGTAGAGAGTCTAGA+GGG | - | chr4.1:83583669-83583688 | MS.gene052955:intron | 40.0% | |
| ! | TCAGACTTTGGCTTCTTCAA+AGG | + | chr4.1:83583000-83583019 | None:intergenic | 40.0% |
| ! | TCTCCTCATCCTTTTTCTTC+TGG | + | chr4.1:83584192-83584211 | None:intergenic | 40.0% |
| ! | TTCTGTTGCATGGATTGAAC+AGG | - | chr4.1:83584284-83584303 | MS.gene052955:intron | 40.0% |
| !!! | TCAGATTAGCCTTTTGGAGT+GGG | - | chr4.1:83583943-83583962 | MS.gene052955:intron | 40.0% |
| !!! | TTCAGATTAGCCTTTTGGAG+TGG | - | chr4.1:83583942-83583961 | MS.gene052955:intron | 40.0% |
| ATTGAGGACTTGAGAGGATG+TGG | - | chr4.1:83583871-83583890 | MS.gene052955:intron | 45.0% | |
| GGATCTGGACTCAAGAAAAG+TGG | - | chr4.1:83584354-83584373 | MS.gene052955:intron | 45.0% | |
| GGTAAGGGTAGGAAGATTTC+AGG | - | chr4.1:83583993-83584012 | MS.gene052955:intron | 45.0% | |
| GGTCGGTCATTCATTTGGTA+AGG | - | chr4.1:83583977-83583996 | MS.gene052955:intron | 45.0% | |
| TAGACGGTCGGTCATTCATT+TGG | - | chr4.1:83583972-83583991 | MS.gene052955:intron | 45.0% | |
| TCCAAAAGATCCCTTCTGTG+AGG | + | chr4.1:83584334-83584353 | None:intergenic | 45.0% | |
| ! | ATGGATTGAACAGGCGCTAA+AGG | - | chr4.1:83584293-83584312 | MS.gene052955:intron | 45.0% |
| ! | TCACAGAAGGGATCTTTTGG+AGG | - | chr4.1:83584333-83584352 | MS.gene052955:intron | 45.0% |
| !! | AAGGGATCTTTTGGAGGATC+TGG | - | chr4.1:83584339-83584358 | MS.gene052955:intron | 45.0% |
| !!! | CAGATTAGCCTTTTGGAGTG+GGG | - | chr4.1:83583944-83583963 | MS.gene052955:intron | 45.0% |
| !!! | GGTGCAGTTTTGTTTGAGCT+TGG | - | chr4.1:83583141-83583160 | MS.gene052955:intron | 45.0% |
| AAGGCTAAGGCCTCACAGAA+GGG | - | chr4.1:83584321-83584340 | MS.gene052955:intron | 50.0% | |
| ATTAGAATGGGAGTGGCTGC+AGG | - | chr4.1:83583846-83583865 | MS.gene052955:intron | 50.0% | |
| GCTAAAGGAGCTGAAGGCTA+AGG | - | chr4.1:83584308-83584327 | MS.gene052955:intron | 50.0% | |
| TTAGAATGGGAGTGGCTGCA+GGG | - | chr4.1:83583847-83583866 | MS.gene052955:intron | 50.0% | |
| ! | GCCTCACAGAAGGGATCTTT+TGG | - | chr4.1:83584330-83584349 | MS.gene052955:intron | 50.0% |
| !! | CAGGGTATTGAGGACTTGAG+AGG | - | chr4.1:83583865-83583884 | MS.gene052955:intron | 50.0% |
| ACAGGCGCTAAAGGAGCTGA+AGG | - | chr4.1:83584302-83584321 | MS.gene052955:intron | 55.0% | |
| CTACCTCTCCCCACTCCAAA+AGG | + | chr4.1:83583955-83583974 | None:intergenic | 55.0% | |
| GAAGGCTAAGGCCTCACAGA+AGG | - | chr4.1:83584320-83584339 | MS.gene052955:intron | 55.0% | |
| TTGGAGTGGGGAGAGGTAGA+CGG | - | chr4.1:83583956-83583975 | MS.gene052955:intron | 55.0% | |
| !!! | TAGCCTTTTGGAGTGGGGAG+AGG | - | chr4.1:83583949-83583968 | MS.gene052955:intron | 55.0% |
| AGTGGGGAGAGGTAGACGGT+CGG | - | chr4.1:83583960-83583979 | MS.gene052955:intron | 60.0% | |
| !! | GGAGTGGCTGCAGGGTATTG+AGG | - | chr4.1:83583855-83583874 | MS.gene052955:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 83582772 | 83584386 | 83582772 | ID=MS.gene052955 |
| chr4.1 | mRNA | 83582772 | 83584386 | 83582772 | ID=MS.gene052955.t1;Parent=MS.gene052955 |
| chr4.1 | exon | 83584371 | 83584386 | 83584371 | ID=MS.gene052955.t1.exon1;Parent=MS.gene052955.t1 |
| chr4.1 | CDS | 83584371 | 83584386 | 83584371 | ID=cds.MS.gene052955.t1;Parent=MS.gene052955.t1 |
| chr4.1 | exon | 83584120 | 83584175 | 83584120 | ID=MS.gene052955.t1.exon2;Parent=MS.gene052955.t1 |
| chr4.1 | CDS | 83584120 | 83584175 | 83584120 | ID=cds.MS.gene052955.t1;Parent=MS.gene052955.t1 |
| chr4.1 | exon | 83582945 | 83582986 | 83582945 | ID=MS.gene052955.t1.exon3;Parent=MS.gene052955.t1 |
| chr4.1 | CDS | 83582945 | 83582986 | 83582945 | ID=cds.MS.gene052955.t1;Parent=MS.gene052955.t1 |
| chr4.1 | exon | 83582772 | 83582852 | 83582772 | ID=MS.gene052955.t1.exon4;Parent=MS.gene052955.t1 |
| chr4.1 | CDS | 83582772 | 83582852 | 83582772 | ID=cds.MS.gene052955.t1;Parent=MS.gene052955.t1 |
| Gene Sequence |
| Protein sequence |
