Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24923.t1 | AES86666.1 | 100 | 58 | 0 | 0 | 1 | 58 | 7 | 64 | 3.10E-19 | 103.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24923.t1 | Q4SUE2 | 60.3 | 58 | 23 | 0 | 1 | 58 | 7 | 64 | 9.6e-09 | 60.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24923.t1 | G7JJD0 | 100.0 | 58 | 0 | 0 | 1 | 58 | 7 | 64 | 2.3e-19 | 103.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049873 | MS.gene24923 | 0.811933 | 5.46E-51 | -1.69E-46 |
| MS.gene050437 | MS.gene24923 | 0.813841 | 2.09E-51 | -1.69E-46 |
| MS.gene050752 | MS.gene24923 | 0.845077 | 5.09E-59 | -1.69E-46 |
| MS.gene050955 | MS.gene24923 | 0.866563 | 2.60E-65 | -1.69E-46 |
| MS.gene051241 | MS.gene24923 | 0.848509 | 5.86E-60 | -1.69E-46 |
| MS.gene05134 | MS.gene24923 | 0.823181 | 1.59E-53 | -1.69E-46 |
| MS.gene052947 | MS.gene24923 | 0.826224 | 3.05E-54 | -1.69E-46 |
| MS.gene052955 | MS.gene24923 | 0.836374 | 9.72E-57 | -1.69E-46 |
| MS.gene053006 | MS.gene24923 | 0.810544 | 1.09E-50 | -1.69E-46 |
| MS.gene05364 | MS.gene24923 | 0.81571 | 8.04E-52 | -1.69E-46 |
| MS.gene053931 | MS.gene24923 | 0.806705 | 7.23E-50 | -1.69E-46 |
| MS.gene054403 | MS.gene24923 | 0.852555 | 4.27E-61 | -1.69E-46 |
| MS.gene05469 | MS.gene24923 | 0.811327 | 7.40E-51 | -1.69E-46 |
| MS.gene05548 | MS.gene24923 | 0.845516 | 3.87E-59 | -1.69E-46 |
| MS.gene055555 | MS.gene24923 | 0.848196 | 7.15E-60 | -1.69E-46 |
| MS.gene055808 | MS.gene24923 | 0.814934 | 1.20E-51 | -1.69E-46 |
| MS.gene056631 | MS.gene24923 | 0.801252 | 9.83E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene24923 | 0.803091 | 4.11E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene24923 | 0.802405 | 5.70E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene24923 | 0.825424 | 4.72E-54 | -1.69E-46 |
| MS.gene057392 | MS.gene24923 | 0.801953 | 7.06E-49 | -1.69E-46 |
| MS.gene057694 | MS.gene24923 | 0.827475 | 1.53E-54 | -1.69E-46 |
| MS.gene057705 | MS.gene24923 | 0.878135 | 3.59E-69 | -1.69E-46 |
| MS.gene05795 | MS.gene24923 | 0.80555 | 1.26E-49 | -1.69E-46 |
| MS.gene058008 | MS.gene24923 | 0.812467 | 4.18E-51 | -1.69E-46 |
| MS.gene058088 | MS.gene24923 | 0.803818 | 2.91E-49 | -1.69E-46 |
| MS.gene058590 | MS.gene24923 | 0.806801 | 6.90E-50 | -1.69E-46 |
| MS.gene058783 | MS.gene24923 | 0.815189 | 1.05E-51 | -1.69E-46 |
| MS.gene05985 | MS.gene24923 | -0.803236 | 3.84E-49 | -1.69E-46 |
| MS.gene060244 | MS.gene24923 | 0.815783 | 7.75E-52 | -1.69E-46 |
| MS.gene060429 | MS.gene24923 | 0.80995 | 1.47E-50 | -1.69E-46 |
| MS.gene060917 | MS.gene24923 | 0.820691 | 6.00E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTCACAGAAGGGATCTTT+TGG | 0.287259 | 4.3:-84494392 | MS.gene24923:CDS |
| GGCTAACATCCAGAAGAAAA+AGG | 0.357013 | 4.3:-84494542 | MS.gene24923:CDS |
| TAATCCTTCTTATCAGACTT+TGG | 0.404695 | 4.3:+84495713 | None:intergenic |
| TCAGACTTTGGCTTCTTCAA+AGG | 0.486773 | 4.3:+84495725 | None:intergenic |
| GAAGCCAAAGTCTGATAAGA+AGG | 0.504619 | 4.3:-84495717 | MS.gene24923:CDS |
| ATGGATTGAACAGGCGCTAA+AGG | 0.513359 | 4.3:-84494429 | MS.gene24923:intron |
| GCTAAAGGAGCTGAAGGCTA+AGG | 0.540771 | 4.3:-84494414 | MS.gene24923:CDS |
| GGATCTGGACTCAAGAAAAG+TGG | 0.544869 | 4.3:-84494368 | MS.gene24923:CDS |
| GAAGGCTAAGGCCTCACAGA+AGG | 0.551780 | 4.3:-84494402 | MS.gene24923:CDS |
| AAGGCTAAGGCCTCACAGAA+GGG | 0.624637 | 4.3:-84494401 | MS.gene24923:CDS |
| ACAGGCGCTAAAGGAGCTGA+AGG | 0.640092 | 4.3:-84494420 | MS.gene24923:intron |
| TCCAAAAGATCCCTTCTGTG+AGG | 0.701774 | 4.3:+84494391 | None:intergenic |
| TGATAAGAAGGATTACGATG+AGG | 0.705327 | 4.3:-84495705 | MS.gene24923:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTAATTTATTTTTTTTT+TGG | - | chr4.3:84494416-84494435 | MS.gene24923:CDS | 0.0% |
| !! | ATAGTAATTATAAACAAAAG+AGG | - | chr4.3:84494642-84494661 | MS.gene24923:intron | 15.0% |
| !! | TAATAAAACCCAAAATTAAA+AGG | + | chr4.3:84494580-84494599 | None:intergenic | 15.0% |
| !!! | TTTTTTTTTGGATATTATGT+TGG | - | chr4.3:84494428-84494447 | MS.gene24923:intron | 15.0% |
| !! | CTAATCAAAGCTATTAATTT+GGG | + | chr4.3:84494890-84494909 | None:intergenic | 20.0% |
| !! | GAATTCTATTAATTCAAACA+TGG | + | chr4.3:84494832-84494851 | None:intergenic | 20.0% |
| !! | TCTAATCAAAGCTATTAATT+TGG | + | chr4.3:84494891-84494910 | None:intergenic | 20.0% |
| !!! | ACTCTAATTCCTTTTAATTT+TGG | - | chr4.3:84494568-84494587 | MS.gene24923:intron | 20.0% |
| !!! | AGAATTGAAATTTTCAAGTT+TGG | - | chr4.3:84494495-84494514 | MS.gene24923:intron | 20.0% |
| !!! | CTCTAATTCCTTTTAATTTT+GGG | - | chr4.3:84494569-84494588 | MS.gene24923:intron | 20.0% |
| !!! | TAATTGTATCTGTATTTTGT+AGG | - | chr4.3:84495525-84495544 | MS.gene24923:intron | 20.0% |
| ! | ACAGATACAATTATATCACA+AGG | + | chr4.3:84495518-84495537 | None:intergenic | 25.0% |
| ! | ACTGTATATTTGAATTGTGT+TGG | - | chr4.3:84494468-84494487 | MS.gene24923:intron | 25.0% |
| ! | ATTGATATTCACACTGAATT+CGG | + | chr4.3:84495154-84495173 | None:intergenic | 25.0% |
| ! | TCATATTCTTCAATCCTTTA+AGG | - | chr4.3:84495180-84495199 | MS.gene24923:intron | 25.0% |
| ! | TGATAGAATATGTTCTACAA+TGG | + | chr4.3:84495396-84495415 | None:intergenic | 25.0% |
| ! | TTTCATATGTGTCTTCTTTA+AGG | - | chr4.3:84494725-84494744 | MS.gene24923:intron | 25.0% |
| !! | AGCTTTGATTAGAAAGTATA+AGG | - | chr4.3:84494898-84494917 | MS.gene24923:intron | 25.0% |
| !! | CTTTGATTAGAAAGTATAAG+GGG | - | chr4.3:84494900-84494919 | MS.gene24923:intron | 25.0% |
| !! | GCTTTGATTAGAAAGTATAA+GGG | - | chr4.3:84494899-84494918 | MS.gene24923:intron | 25.0% |
| !! | TTTTTGTTGCATTTTACTTG+AGG | - | chr4.3:84495480-84495499 | MS.gene24923:intron | 25.0% |
| !!! | CGAATTCTGTTTTTTTTCAA+CGG | + | chr4.3:84494790-84494809 | None:intergenic | 25.0% |
| !!! | TATTGGGTGTTTTTGTTATA+GGG | - | chr4.3:84494545-84494564 | MS.gene24923:CDS | 25.0% |
| !!! | TTATTGGGTGTTTTTGTTAT+AGG | - | chr4.3:84494544-84494563 | MS.gene24923:CDS | 25.0% |
| AAGCAAAAGGATAGAAGAAA+TGG | + | chr4.3:84495130-84495149 | None:intergenic | 30.0% | |
| ACCATACCTAGAAATTAGAA+TGG | - | chr4.3:84495208-84495227 | MS.gene24923:intron | 30.0% | |
| CAACAGAATCAGGTAAAAAT+AGG | + | chr4.3:84495647-84495666 | None:intergenic | 30.0% | |
| CAAGAAAAGTGGAAAGAAAT+AGG | - | chr4.3:84495740-84495759 | MS.gene24923:CDS | 30.0% | |
| CCATACCTAGAAATTAGAAT+GGG | - | chr4.3:84495209-84495228 | MS.gene24923:intron | 30.0% | |
| GGATATTAATGACAGACAAT+AGG | - | chr4.3:84494932-84494951 | MS.gene24923:intron | 30.0% | |
| GGTAAGAAGGAAATATACAT+AGG | - | chr4.3:84494984-84495003 | MS.gene24923:intron | 30.0% | |
| GTGGAATATTGTGTTAAGAA+TGG | - | chr4.3:84495265-84495284 | MS.gene24923:intron | 30.0% | |
| TAATCCTTCTTATCAGACTT+TGG | + | chr4.3:84494387-84494406 | None:intergenic | 30.0% | |
| TATGAAAAATCAGCAAACAG+TGG | + | chr4.3:84494712-84494731 | None:intergenic | 30.0% | |
| TTGAGATTTCTGCTGTTAAT+TGG | - | chr4.3:84495445-84495464 | MS.gene24923:intron | 30.0% | |
| ! | AAGGCTAATCTGAAATATGT+TGG | + | chr4.3:84495311-84495330 | None:intergenic | 30.0% |
| !! | AAAGTATAAGGGGAGTATTT+GGG | - | chr4.3:84494910-84494929 | MS.gene24923:intron | 30.0% |
| !! | TTGAGCTTGGTTGTTTTATT+GGG | - | chr4.3:84494529-84494548 | MS.gene24923:intron | 30.0% |
| !! | TTTGAGCTTGGTTGTTTTAT+TGG | - | chr4.3:84494528-84494547 | MS.gene24923:intron | 30.0% |
| !!! | CATATTTCAGATTAGCCTTT+TGG | - | chr4.3:84495312-84495331 | MS.gene24923:intron | 30.0% |
| AAAAATCAGCAAACAGTGGT+AGG | + | chr4.3:84494708-84494727 | None:intergenic | 35.0% | |
| AACAGAATTAGGAGGTAAGA+AGG | - | chr4.3:84494971-84494990 | MS.gene24923:intron | 35.0% | |
| CAGGTTTGCATAACAGAATT+AGG | - | chr4.3:84494960-84494979 | MS.gene24923:intron | 35.0% | |
| GTTTGCATAACAGAATTAGG+AGG | - | chr4.3:84494963-84494982 | MS.gene24923:intron | 35.0% | |
| TGACAGACAATAGGATATTC+AGG | - | chr4.3:84494941-84494960 | MS.gene24923:intron | 35.0% | |
| TGATAAGAAGGATTACGATG+AGG | - | chr4.3:84494392-84494411 | MS.gene24923:CDS | 35.0% | |
| TTGAAAGTCATGTCTCATGA+GGG | - | chr4.3:84495078-84495097 | MS.gene24923:intron | 35.0% | |
| TTTGAAAGTCATGTCTCATG+AGG | - | chr4.3:84495077-84495096 | MS.gene24923:intron | 35.0% | |
| ! | CCCATTCTAATTTCTAGGTA+TGG | + | chr4.3:84495212-84495231 | None:intergenic | 35.0% |
| ! | TTTTACCTGATTCTGTTGCA+TGG | - | chr4.3:84495649-84495668 | MS.gene24923:intron | 35.0% |
| !! | AAGTATAAGGGGAGTATTTG+GGG | - | chr4.3:84494911-84494930 | MS.gene24923:intron | 35.0% |
| !! | CTGTATTTTGTAGGTTGACA+TGG | - | chr4.3:84495534-84495553 | MS.gene24923:intron | 35.0% |
| !! | GAAAGTATAAGGGGAGTATT+TGG | - | chr4.3:84494909-84494928 | MS.gene24923:intron | 35.0% |
| AGGTATGGTAGTTGCCTTAA+AGG | + | chr4.3:84495197-84495216 | None:intergenic | 40.0% | |
| ATGTAGAGTAGAGAGTCTAG+AGG | - | chr4.3:84495043-84495062 | MS.gene24923:intron | 40.0% | |
| CACTGAATTCGGTAAGCAAA+AGG | + | chr4.3:84495143-84495162 | None:intergenic | 40.0% | |
| CATCCAGAAGAAAAAGGATG+AGG | - | chr4.3:84495561-84495580 | MS.gene24923:intron | 40.0% | |
| CCACTCCCATTCTAATTTCT+AGG | + | chr4.3:84495217-84495236 | None:intergenic | 40.0% | |
| CCTAGAAATTAGAATGGGAG+TGG | - | chr4.3:84495214-84495233 | MS.gene24923:intron | 40.0% | |
| GAAGAAAAAGGATGAGGAGA+AGG | - | chr4.3:84495567-84495586 | MS.gene24923:intron | 40.0% | |
| GAAGCCAAAGTCTGATAAGA+AGG | - | chr4.3:84494380-84494399 | MS.gene24923:CDS | 40.0% | |
| GGCTAACATCCAGAAGAAAA+AGG | - | chr4.3:84495555-84495574 | MS.gene24923:intron | 40.0% | |
| GTAAGGGTAGGAAGATTTCA+GGG | - | chr4.3:84495369-84495388 | MS.gene24923:intron | 40.0% | |
| GTCATTCATTTGGTAAGGGT+AGG | - | chr4.3:84495357-84495376 | MS.gene24923:intron | 40.0% | |
| GTCGGTCATTCATTTGGTAA+GGG | - | chr4.3:84495353-84495372 | MS.gene24923:intron | 40.0% | |
| TCAATCCATGCAACAGAATC+AGG | + | chr4.3:84495657-84495676 | None:intergenic | 40.0% | |
| TGTAGAGTAGAGAGTCTAGA+GGG | - | chr4.3:84495044-84495063 | MS.gene24923:intron | 40.0% | |
| ! | TCAGACTTTGGCTTCTTCAA+AGG | + | chr4.3:84494375-84494394 | None:intergenic | 40.0% |
| ! | TCTCCTCATCCTTTTTCTTC+TGG | + | chr4.3:84495567-84495586 | None:intergenic | 40.0% |
| ! | TTCTGTTGCATGGATTGAAC+AGG | - | chr4.3:84495659-84495678 | MS.gene24923:intron | 40.0% |
| !!! | TCAGATTAGCCTTTTGGAGT+GGG | - | chr4.3:84495318-84495337 | MS.gene24923:intron | 40.0% |
| !!! | TTCAGATTAGCCTTTTGGAG+TGG | - | chr4.3:84495317-84495336 | MS.gene24923:intron | 40.0% |
| ATTGAGGACTTGAGAGGATG+TGG | - | chr4.3:84495246-84495265 | MS.gene24923:intron | 45.0% | |
| GGATCTGGACTCAAGAAAAG+TGG | - | chr4.3:84495729-84495748 | MS.gene24923:CDS | 45.0% | |
| GGTAAGGGTAGGAAGATTTC+AGG | - | chr4.3:84495368-84495387 | MS.gene24923:intron | 45.0% | |
| GGTCGGTCATTCATTTGGTA+AGG | - | chr4.3:84495352-84495371 | MS.gene24923:intron | 45.0% | |
| TAGACGGTCGGTCATTCATT+TGG | - | chr4.3:84495347-84495366 | MS.gene24923:intron | 45.0% | |
| TCCAAAAGATCCCTTCTGTG+AGG | + | chr4.3:84495709-84495728 | None:intergenic | 45.0% | |
| ! | ATGGATTGAACAGGCGCTAA+AGG | - | chr4.3:84495668-84495687 | MS.gene24923:intron | 45.0% |
| ! | TCACAGAAGGGATCTTTTGG+AGG | - | chr4.3:84495708-84495727 | MS.gene24923:CDS | 45.0% |
| !! | AAGGGATCTTTTGGAGGATC+TGG | - | chr4.3:84495714-84495733 | MS.gene24923:CDS | 45.0% |
| !!! | CAGATTAGCCTTTTGGAGTG+GGG | - | chr4.3:84495319-84495338 | MS.gene24923:intron | 45.0% |
| !!! | GGTGCAGTTTTGTTTGAGCT+TGG | - | chr4.3:84494516-84494535 | MS.gene24923:intron | 45.0% |
| AAGGCTAAGGCCTCACAGAA+GGG | - | chr4.3:84495696-84495715 | MS.gene24923:intron | 50.0% | |
| ATTAGAATGGGAGTGGCTGC+AGG | - | chr4.3:84495221-84495240 | MS.gene24923:intron | 50.0% | |
| GCTAAAGGAGCTGAAGGCTA+AGG | - | chr4.3:84495683-84495702 | MS.gene24923:intron | 50.0% | |
| TTAGAATGGGAGTGGCTGCA+GGG | - | chr4.3:84495222-84495241 | MS.gene24923:intron | 50.0% | |
| ! | GCCTCACAGAAGGGATCTTT+TGG | - | chr4.3:84495705-84495724 | MS.gene24923:intron | 50.0% |
| ACAGGCGCTAAAGGAGCTGA+AGG | - | chr4.3:84495677-84495696 | MS.gene24923:intron | 55.0% | |
| CTACCTCTCCCCACTCCAAA+AGG | + | chr4.3:84495330-84495349 | None:intergenic | 55.0% | |
| GAAGGCTAAGGCCTCACAGA+AGG | - | chr4.3:84495695-84495714 | MS.gene24923:intron | 55.0% | |
| TTGGAGTGGGGAGAGGTAGA+CGG | - | chr4.3:84495331-84495350 | MS.gene24923:intron | 55.0% | |
| !! | CAGGGCATTGAGGACTTGAG+AGG | - | chr4.3:84495240-84495259 | MS.gene24923:intron | 55.0% |
| !!! | TAGCCTTTTGGAGTGGGGAG+AGG | - | chr4.3:84495324-84495343 | MS.gene24923:intron | 55.0% |
| AGTGGGGAGAGGTAGACGGT+CGG | - | chr4.3:84495335-84495354 | MS.gene24923:intron | 60.0% | |
| !! | GGAGTGGCTGCAGGGCATTG+AGG | - | chr4.3:84495230-84495249 | MS.gene24923:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 84494358 | 84495761 | 84494358 | ID=MS.gene24923 |
| chr4.3 | mRNA | 84494358 | 84495761 | 84494358 | ID=MS.gene24923.t1;Parent=MS.gene24923 |
| chr4.3 | exon | 84495706 | 84495761 | 84495706 | ID=MS.gene24923.t1.exon1;Parent=MS.gene24923.t1 |
| chr4.3 | CDS | 84495706 | 84495761 | 84495706 | ID=cds.MS.gene24923.t1;Parent=MS.gene24923.t1 |
| chr4.3 | exon | 84494531 | 84494572 | 84494531 | ID=MS.gene24923.t1.exon2;Parent=MS.gene24923.t1 |
| chr4.3 | CDS | 84494531 | 84494572 | 84494531 | ID=cds.MS.gene24923.t1;Parent=MS.gene24923.t1 |
| chr4.3 | exon | 84494358 | 84494438 | 84494358 | ID=MS.gene24923.t1.exon3;Parent=MS.gene24923.t1 |
| chr4.3 | CDS | 84494358 | 84494438 | 84494358 | ID=cds.MS.gene24923.t1;Parent=MS.gene24923.t1 |
| Gene Sequence |
| Protein sequence |
