Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053566.t1 | RHN62674.1 | 95.3 | 86 | 3 | 1 | 1 | 86 | 76 | 160 | 1.60E-35 | 158.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053566.t1 | G7JV68 | 95.3 | 86 | 3 | 1 | 1 | 86 | 1 | 85 | 1.2e-35 | 158.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053566 | MS.gene065667 | 0.824122 | 9.58E-54 | -1.69E-46 |
| MS.gene053566 | MS.gene06645 | 0.801234 | 9.91E-49 | -1.69E-46 |
| MS.gene053566 | MS.gene071988 | 0.809964 | 1.46E-50 | -1.69E-46 |
| MS.gene053566 | MS.gene34106 | 0.804186 | 2.44E-49 | -1.69E-46 |
| MS.gene053566 | MS.gene71704 | 0.815423 | 9.32E-52 | -1.69E-46 |
| MS.gene053566 | MS.gene73527 | 0.802765 | 4.80E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053566.t1 | MTR_4g093890 | 100.000 | 75 | 0 | 0 | 1 | 75 | 1 | 75 | 1.87e-48 | 148 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACACAAACATTTCCTGATT+TGG | 0.225196 | 4.4:-21259576 | MS.gene053566:CDS |
| GAAGTCAGGAATATTGAAAA+TGG | 0.251652 | 4.4:+21259481 | None:intergenic |
| CCTGATTTGGGCAAAATTCC+AGG | 0.274023 | 4.4:-21259563 | MS.gene053566:CDS |
| ACACAAACATTTCCTGATTT+GGG | 0.316697 | 4.4:-21259575 | MS.gene053566:CDS |
| TACTCTGGCCATTCAGTCAC+TGG | 0.448656 | 4.4:+21259518 | None:intergenic |
| CTTTCCCACCAGTGACTGAA+TGG | 0.466160 | 4.4:-21259526 | MS.gene053566:CDS |
| ACCTTGGACATTGTCAATGC+TGG | 0.468209 | 4.4:+21259619 | None:intergenic |
| ATTGACAATGTCCAAGGTGG+TGG | 0.469460 | 4.4:-21259614 | MS.gene053566:CDS |
| GATGGTGGAGAGAAGAAGTC+AGG | 0.493659 | 4.4:+21259467 | None:intergenic |
| AAGTCAGGAATATTGAAAAT+GGG | 0.495802 | 4.4:+21259482 | None:intergenic |
| TGACTGAATGGCCAGAGTAC+AGG | 0.500741 | 4.4:-21259514 | MS.gene053566:CDS |
| CATTCAGTCACTGGTGGGAA+AGG | 0.519050 | 4.4:+21259527 | None:intergenic |
| AAAGGAGGAAACTCTAGCCC+TGG | 0.530197 | 4.4:+21259545 | None:intergenic |
| CTGATTTGGGCAAAATTCCA+GGG | 0.550481 | 4.4:-21259562 | MS.gene053566:CDS |
| GGTGGAGGCAACCTGTACTC+TGG | 0.553702 | 4.4:+21259503 | None:intergenic |
| GTTGTGGTGGTGTCAGATGG+TGG | 0.556621 | 4.4:+21259452 | None:intergenic |
| GTTGTTGTGGTGGTGTCAGA+TGG | 0.562955 | 4.4:+21259449 | None:intergenic |
| TCAGTCACTGGTGGGAAAGG+AGG | 0.574402 | 4.4:+21259530 | None:intergenic |
| TCCAGCATTGACAATGTCCA+AGG | 0.592453 | 4.4:-21259620 | MS.gene053566:CDS |
| TCTGGCCATTCAGTCACTGG+TGG | 0.608143 | 4.4:+21259521 | None:intergenic |
| TCAGGAATATTGAAAATGGG+TGG | 0.625430 | 4.4:+21259485 | None:intergenic |
| CTGGCCATTCAGTCACTGGT+GGG | 0.629336 | 4.4:+21259522 | None:intergenic |
| GGAATATTGAAAATGGGTGG+AGG | 0.630069 | 4.4:+21259488 | None:intergenic |
| TTGACAATGTCCAAGGTGGT+GGG | 0.635337 | 4.4:-21259613 | MS.gene053566:CDS |
| AGCATTGACAATGTCCAAGG+TGG | 0.725622 | 4.4:-21259617 | MS.gene053566:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAGCTAACAAAAACAAA+CGG | + | chr4.4:21259447-21259466 | None:intergenic | 20.0% |
| ! | AAGTCAGGAATATTGAAAAT+GGG | + | chr4.4:21259625-21259644 | None:intergenic | 25.0% |
| ACACAAACATTTCCTGATTT+GGG | - | chr4.4:21259529-21259548 | MS.gene053566:CDS | 30.0% | |
| GAAGTCAGGAATATTGAAAA+TGG | + | chr4.4:21259626-21259645 | None:intergenic | 30.0% | |
| GACACAAACATTTCCTGATT+TGG | - | chr4.4:21259528-21259547 | MS.gene053566:CDS | 35.0% | |
| TAACAAAAACAAACGGACAG+AGG | + | chr4.4:21259440-21259459 | None:intergenic | 35.0% | |
| TCAGGAATATTGAAAATGGG+TGG | + | chr4.4:21259622-21259641 | None:intergenic | 35.0% | |
| GGAATATTGAAAATGGGTGG+AGG | + | chr4.4:21259619-21259638 | None:intergenic | 40.0% | |
| ! | TTTTGTAGCAGTTGTTGTGG+TGG | + | chr4.4:21259668-21259687 | None:intergenic | 40.0% |
| !! | CTGATTTGGGCAAAATTCCA+GGG | - | chr4.4:21259542-21259561 | MS.gene053566:CDS | 40.0% |
| !!! | TGGTTTTGTAGCAGTTGTTG+TGG | + | chr4.4:21259671-21259690 | None:intergenic | 40.0% |
| ACCTTGGACATTGTCAATGC+TGG | + | chr4.4:21259488-21259507 | None:intergenic | 45.0% | |
| ATTGACAATGTCCAAGGTGG+TGG | - | chr4.4:21259490-21259509 | MS.gene053566:CDS | 45.0% | |
| TTGACAATGTCCAAGGTGGT+GGG | - | chr4.4:21259491-21259510 | MS.gene053566:CDS | 45.0% | |
| ! | CCTGGAATTTTGCCCAAATC+AGG | + | chr4.4:21259544-21259563 | None:intergenic | 45.0% |
| !! | AGCATTGACAATGTCCAAGG+TGG | - | chr4.4:21259487-21259506 | MS.gene053566:CDS | 45.0% |
| !! | CCTGATTTGGGCAAAATTCC+AGG | - | chr4.4:21259541-21259560 | MS.gene053566:CDS | 45.0% |
| !! | TCCAGCATTGACAATGTCCA+AGG | - | chr4.4:21259484-21259503 | MS.gene053566:CDS | 45.0% |
| AAAGGAGGAAACTCTAGCCC+TGG | + | chr4.4:21259562-21259581 | None:intergenic | 50.0% | |
| CATTCAGTCACTGGTGGGAA+AGG | + | chr4.4:21259580-21259599 | None:intergenic | 50.0% | |
| CTTTCCCACCAGTGACTGAA+TGG | - | chr4.4:21259578-21259597 | MS.gene053566:CDS | 50.0% | |
| TACTCTGGCCATTCAGTCAC+TGG | + | chr4.4:21259589-21259608 | None:intergenic | 50.0% | |
| TGACTGAATGGCCAGAGTAC+AGG | - | chr4.4:21259590-21259609 | MS.gene053566:CDS | 50.0% | |
| ! | GTTGTTGTGGTGGTGTCAGA+TGG | + | chr4.4:21259658-21259677 | None:intergenic | 50.0% |
| !! | GATGGTGGAGAGAAGAAGTC+AGG | + | chr4.4:21259640-21259659 | None:intergenic | 50.0% |
| !! | GTTTTCTAGTCCCACCACCT+TGG | + | chr4.4:21259504-21259523 | None:intergenic | 50.0% |
| CTGGCCATTCAGTCACTGGT+GGG | + | chr4.4:21259585-21259604 | None:intergenic | 55.0% | |
| TCAGTCACTGGTGGGAAAGG+AGG | + | chr4.4:21259577-21259596 | None:intergenic | 55.0% | |
| TCTGGCCATTCAGTCACTGG+TGG | + | chr4.4:21259586-21259605 | None:intergenic | 55.0% | |
| ! | GTTGTGGTGGTGTCAGATGG+TGG | + | chr4.4:21259655-21259674 | None:intergenic | 55.0% |
| GGTGGAGGCAACCTGTACTC+TGG | + | chr4.4:21259604-21259623 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 21259433 | 21259693 | 21259433 | ID=MS.gene053566 |
| chr4.4 | mRNA | 21259433 | 21259693 | 21259433 | ID=MS.gene053566.t1;Parent=MS.gene053566 |
| chr4.4 | exon | 21259433 | 21259693 | 21259433 | ID=MS.gene053566.t1.exon1;Parent=MS.gene053566.t1 |
| chr4.4 | CDS | 21259433 | 21259693 | 21259433 | ID=cds.MS.gene053566.t1;Parent=MS.gene053566.t1 |
| Gene Sequence |
| Protein sequence |
