AlfalfaGEDB | MS.gene73527

M. sativa cultivar XinJiangDaYe / MS.gene73527

KEGG: K03626
GO: -
PFAM: PF01849.21 PF19026.3

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73527.t1	XP_003624883.1	96.4	110	4	0	7	116	94	203	1.40E-47	198.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73527.t1	Q9M612	78.2	110	23	1	7	115	95	204	2.6e-37	156.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73527.t1	G7L4T7	96.4	110	4	0	7	116	94	203	1.0e-47	198.7

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051247	MS.gene73527	0.816148	6.42E-52	-1.69E-46
MS.gene05157	MS.gene73527	0.801624	8.25E-49	-1.69E-46
MS.gene051744	MS.gene73527	0.804319	2.29E-49	-1.69E-46
MS.gene053226	MS.gene73527	0.817467	3.25E-52	-1.69E-46
MS.gene053566	MS.gene73527	0.802765	4.80E-49	-1.69E-46
MS.gene054236	MS.gene73527	0.807261	5.51E-50	-1.69E-46
MS.gene054775	MS.gene73527	0.813554	2.41E-51	-1.69E-46
MS.gene054801	MS.gene73527	0.813938	1.99E-51	-1.69E-46
MS.gene055466	MS.gene73527	0.817559	3.10E-52	-1.69E-46
MS.gene057693	MS.gene73527	0.805337	1.40E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73527.t1	MTR_7g088680	96.364	110	4	0	7	116	94	203	5.60e-72	213
MS.gene73527.t1	MTR_3g092970	66.379	116	33	2	7	116	112	227	2.16e-42	139
MS.gene73527.t1	MTR_3g092980	55.285	123	34	2	7	116	104	218	8.50e-36	122
MS.gene73527.t1	MTR_3g092980	55.285	123	34	2	7	116	106	220	1.04e-35	122
MS.gene73527.t1	MTR_3g093750	55.752	113	37	4	7	116	425	527	1.71e-25	99.8
MS.gene73527.t1	MTR_3g093720	76.744	43	10	0	74	116	215	257	4.59e-15	69.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73527.t1	AT3G12390	73.214	112	28	1	7	116	92	203	2.69e-42	138
MS.gene73527.t1	AT5G13850	69.828	116	28	1	7	115	88	203	2.17e-40	133
MS.gene73527.t1	AT3G49470	60.345	116	40	1	7	116	102	217	1.18e-38	129
MS.gene73527.t1	AT1G33040	59.130	115	41	2	7	115	94	208	1.66e-36	123
MS.gene73527.t1	AT4G10480	62.393	117	34	2	7	115	96	210	8.45e-36	122
MS.gene73527.t1	AT4G10480	62.393	117	34	2	7	115	97	211	9.26e-36	122
MS.gene73527.t1	AT3G49470	58.879	107	38	1	7	107	102	208	2.87e-33	115

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Find 27 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTCTCAAAGCTGTCAA+TGG	0.508744	4.4:-64868380	MS.gene73527:CDS
ACCACCAACTCAATATCCTT+GGG	0.531886	4.4:+64868437	None:intergenic
AGACATTGTTGCTGCCATTA+TGG	0.359211	4.4:-64868357	MS.gene73527:CDS
AGATCCCAAGGATATTGAGT+TGG	0.629225	4.4:-64868441	MS.gene73527:CDS
AGCTTTCCCAAATATAATGT+AGG	0.558527	4.4:+64868601	None:intergenic
ATAATGTAGGTGTCAGAAGT+TGG	0.511639	4.4:+64868614	None:intergenic
ATCTTCTGGAGCAATACCAG+AGG	0.670950	4.4:+64868487	None:intergenic
CACCACCAACTCAATATCCT+TGG	0.437037	4.4:+64868436	None:intergenic
CGAGACTGGTGTAGATCCCA+AGG	0.607645	4.4:-64868453	MS.gene73527:CDS
CTGACACCTACATTATATTT+GGG	0.330139	4.4:-64868607	MS.gene73527:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGCTTTCCCAAATATAATGT+AGG	+	chr4.4:64868527-64868546	None:intergenic	30.0%
CTGACACCTACATTATATTT+GGG	-	chr4.4:64868518-64868537	MS.gene73527:CDS	30.0%
TCTGACACCTACATTATATT+TGG	-	chr4.4:64868517-64868536	MS.gene73527:CDS	30.0%
ATAATGTAGGTGTCAGAAGT+TGG	+	chr4.4:64868514-64868533	None:intergenic	35.0%
AAAGCTCTCAAAGCTGTCAA+TGG	-	chr4.4:64868745-64868764	MS.gene73527:intron	40.0%
ACCACCAACTCAATATCCTT+GGG	+	chr4.4:64868691-64868710	None:intergenic	40.0%
AGACATTGTTGCTGCCATTA+TGG	-	chr4.4:64868768-64868787	MS.gene73527:intron	40.0%
AGATCCCAAGGATATTGAGT+TGG	-	chr4.4:64868684-64868703	MS.gene73527:intron	40.0%
ATGAACTGAGGCTACAATGA+TGG	-	chr4.4:64868379-64868398	MS.gene73527:CDS	40.0%
GATCCTAATTTGACCAATGC+GGG	-	chr4.4:64868601-64868620	MS.gene73527:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	64868343	64868804	64868343	ID=MS.gene73527
chr4.4	mRNA	64868343	64868804	64868343	ID=MS.gene73527.t1;Parent=MS.gene73527
chr4.4	exon	64868787	64868804	64868787	ID=MS.gene73527.t1.exon1;Parent=MS.gene73527.t1
chr4.4	CDS	64868787	64868804	64868787	ID=cds.MS.gene73527.t1;Parent=MS.gene73527.t1
chr4.4	exon	64868343	64868675	64868343	ID=MS.gene73527.t1.exon2;Parent=MS.gene73527.t1
chr4.4	CDS	64868343	64868675	64868343	ID=cds.MS.gene73527.t1;Parent=MS.gene73527.t1

Gene Sequence

>MS.gene73527

ATGACTTTCAATTATGCTGTTGTTTTTATTTTTTGCATGAACTGAGGCTACAATGATGGATACAATATTTTTTTCTTCTTCTTTTGGAATGCGTCTAAATTTAAAAGTTGCTTTCTACTTGTTCATCAGATCTTGTTCGTCATCTCAAAACCAGATGTTTTCAAGAGTCCAACTTCTGACACCTACATTATATTTGGGAAAGCTAAGATTGAATACTTGAGCTCACAGCTACAAACTCAGGCTGCAGAGCAGTTCAAGGATCCTAATTTGACCAATGCGGGTGCGAAGCCAGAATCCTCTGGTATTGCTCCAGAAGATGAGGATGTAGACGAGACTGGTGTAGATCCCAAGGATATTGAGTTGGTGGTGACTCAAGCTGGTGTGCCAAGATCAAGAGCTGTTAAAGCTCTCAAAGCTGTCAATGGAGACATTGTTGCTGCCATTATGGAGCTAACAAATTAA

Protein sequence

>MS.gene73527.t1

MTFNYAILFVISKPDVFKSPTSDTYIIFGKAKIEYLSSQLQTQAAEQFKDPNLTNAGAKPESSGIAPEDEDVDETGVDPKDIELVVTQAGVPRSRAVKALKAVNGDIVAAIMELTN