Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73527.t1 | XP_003624883.1 | 96.4 | 110 | 4 | 0 | 7 | 116 | 94 | 203 | 1.40E-47 | 198.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73527.t1 | Q9M612 | 78.2 | 110 | 23 | 1 | 7 | 115 | 95 | 204 | 2.6e-37 | 156.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73527.t1 | G7L4T7 | 96.4 | 110 | 4 | 0 | 7 | 116 | 94 | 203 | 1.0e-47 | 198.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051247 | MS.gene73527 | 0.816148 | 6.42E-52 | -1.69E-46 |
| MS.gene05157 | MS.gene73527 | 0.801624 | 8.25E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene73527 | 0.804319 | 2.29E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene73527 | 0.817467 | 3.25E-52 | -1.69E-46 |
| MS.gene053566 | MS.gene73527 | 0.802765 | 4.80E-49 | -1.69E-46 |
| MS.gene054236 | MS.gene73527 | 0.807261 | 5.51E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene73527 | 0.813554 | 2.41E-51 | -1.69E-46 |
| MS.gene054801 | MS.gene73527 | 0.813938 | 1.99E-51 | -1.69E-46 |
| MS.gene055466 | MS.gene73527 | 0.817559 | 3.10E-52 | -1.69E-46 |
| MS.gene057693 | MS.gene73527 | 0.805337 | 1.40E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene73527 | 0.800641 | 1.31E-48 | -1.69E-46 |
| MS.gene060429 | MS.gene73527 | 0.80742 | 5.10E-50 | -1.69E-46 |
| MS.gene060803 | MS.gene73527 | 0.818357 | 2.04E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73527.t1 | MTR_7g088680 | 96.364 | 110 | 4 | 0 | 7 | 116 | 94 | 203 | 5.60e-72 | 213 |
| MS.gene73527.t1 | MTR_3g092970 | 66.379 | 116 | 33 | 2 | 7 | 116 | 112 | 227 | 2.16e-42 | 139 |
| MS.gene73527.t1 | MTR_3g092980 | 55.285 | 123 | 34 | 2 | 7 | 116 | 104 | 218 | 8.50e-36 | 122 |
| MS.gene73527.t1 | MTR_3g092980 | 55.285 | 123 | 34 | 2 | 7 | 116 | 106 | 220 | 1.04e-35 | 122 |
| MS.gene73527.t1 | MTR_3g093750 | 55.752 | 113 | 37 | 4 | 7 | 116 | 425 | 527 | 1.71e-25 | 99.8 |
| MS.gene73527.t1 | MTR_3g093720 | 76.744 | 43 | 10 | 0 | 74 | 116 | 215 | 257 | 4.59e-15 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73527.t1 | AT3G12390 | 73.214 | 112 | 28 | 1 | 7 | 116 | 92 | 203 | 2.69e-42 | 138 |
| MS.gene73527.t1 | AT5G13850 | 69.828 | 116 | 28 | 1 | 7 | 115 | 88 | 203 | 2.17e-40 | 133 |
| MS.gene73527.t1 | AT3G49470 | 60.345 | 116 | 40 | 1 | 7 | 116 | 102 | 217 | 1.18e-38 | 129 |
| MS.gene73527.t1 | AT1G33040 | 59.130 | 115 | 41 | 2 | 7 | 115 | 94 | 208 | 1.66e-36 | 123 |
| MS.gene73527.t1 | AT4G10480 | 62.393 | 117 | 34 | 2 | 7 | 115 | 96 | 210 | 8.45e-36 | 122 |
| MS.gene73527.t1 | AT4G10480 | 62.393 | 117 | 34 | 2 | 7 | 115 | 97 | 211 | 9.26e-36 | 122 |
| MS.gene73527.t1 | AT3G49470 | 58.879 | 107 | 38 | 1 | 7 | 107 | 102 | 208 | 2.87e-33 | 115 |
Find 27 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGACACCTACATTATATT+TGG | 0.098409 | 4.4:-64868608 | MS.gene73527:CDS |
| GGAGCAATACCAGAGGATTC+TGG | 0.324111 | 4.4:+64868494 | None:intergenic |
| TCGTCTACATCCTCATCTTC+TGG | 0.324398 | 4.4:+64868473 | None:intergenic |
| CTGACACCTACATTATATTT+GGG | 0.330139 | 4.4:-64868607 | MS.gene73527:CDS |
| GCACCCGCATTGGTCAAATT+AGG | 0.333932 | 4.4:+64868521 | None:intergenic |
| GCTTTAACAGCTCTTGATCT+TGG | 0.356165 | 4.4:+64868398 | None:intergenic |
| AGACATTGTTGCTGCCATTA+TGG | 0.359211 | 4.4:-64868357 | MS.gene73527:CDS |
| GTTGGACTCTTGAAAACATC+TGG | 0.370830 | 4.4:+64868632 | None:intergenic |
| TTAATTTGTTAGCTCCATAA+TGG | 0.425574 | 4.4:+64868343 | None:intergenic |
| CACCACCAACTCAATATCCT+TGG | 0.437037 | 4.4:+64868436 | None:intergenic |
| TTCTGGCTTCGCACCCGCAT+TGG | 0.469966 | 4.4:+64868511 | None:intergenic |
| TCAGGCTGCAGAGCAGTTCA+AGG | 0.493163 | 4.4:-64868546 | MS.gene73527:CDS |
| AAAGCTCTCAAAGCTGTCAA+TGG | 0.508744 | 4.4:-64868380 | MS.gene73527:CDS |
| ATAATGTAGGTGTCAGAAGT+TGG | 0.511639 | 4.4:+64868614 | None:intergenic |
| TCCCAAGGATATTGAGTTGG+TGG | 0.515624 | 4.4:-64868438 | MS.gene73527:CDS |
| GATCCTAATTTGACCAATGC+GGG | 0.519700 | 4.4:-64868524 | MS.gene73527:CDS |
| ACCACCAACTCAATATCCTT+GGG | 0.531886 | 4.4:+64868437 | None:intergenic |
| GGTGCGAAGCCAGAATCCTC+TGG | 0.535151 | 4.4:-64868503 | MS.gene73527:CDS |
| GATGAGGATGTAGACGAGAC+TGG | 0.538671 | 4.4:-64868467 | MS.gene73527:CDS |
| GAGTTGGTGGTGACTCAAGC+TGG | 0.556994 | 4.4:-64868425 | MS.gene73527:CDS |
| AGCTTTCCCAAATATAATGT+AGG | 0.558527 | 4.4:+64868601 | None:intergenic |
| GAGCTCACAGCTACAAACTC+AGG | 0.597975 | 4.4:-64868564 | MS.gene73527:CDS |
| CGAGACTGGTGTAGATCCCA+AGG | 0.607645 | 4.4:-64868453 | MS.gene73527:CDS |
| TGGTATTGCTCCAGAAGATG+AGG | 0.607813 | 4.4:-64868483 | MS.gene73527:CDS |
| AGATCCCAAGGATATTGAGT+TGG | 0.629225 | 4.4:-64868441 | MS.gene73527:CDS |
| ATCTTCTGGAGCAATACCAG+AGG | 0.670950 | 4.4:+64868487 | None:intergenic |
| GGATCCTAATTTGACCAATG+CGG | 0.677685 | 4.4:-64868525 | MS.gene73527:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTTTTTTCTTCTTCTTT+TGG | - | chr4.4:64868407-64868426 | MS.gene73527:CDS | 15.0% |
| !!! | TTAATTTGTTAGCTCCATAA+TGG | + | chr4.4:64868785-64868804 | None:intergenic | 25.0% |
| !!! | TTTATTTTTTGCATGAACTG+AGG | - | chr4.4:64868367-64868386 | MS.gene73527:CDS | 25.0% |
| AGCTTTCCCAAATATAATGT+AGG | + | chr4.4:64868527-64868546 | None:intergenic | 30.0% | |
| CTGACACCTACATTATATTT+GGG | - | chr4.4:64868518-64868537 | MS.gene73527:CDS | 30.0% | |
| TCTGACACCTACATTATATT+TGG | - | chr4.4:64868517-64868536 | MS.gene73527:CDS | 30.0% | |
| ATAATGTAGGTGTCAGAAGT+TGG | + | chr4.4:64868514-64868533 | None:intergenic | 35.0% | |
| AAAGCTCTCAAAGCTGTCAA+TGG | - | chr4.4:64868745-64868764 | MS.gene73527:intron | 40.0% | |
| ACCACCAACTCAATATCCTT+GGG | + | chr4.4:64868691-64868710 | None:intergenic | 40.0% | |
| AGACATTGTTGCTGCCATTA+TGG | - | chr4.4:64868768-64868787 | MS.gene73527:intron | 40.0% | |
| AGATCCCAAGGATATTGAGT+TGG | - | chr4.4:64868684-64868703 | MS.gene73527:intron | 40.0% | |
| ATGAACTGAGGCTACAATGA+TGG | - | chr4.4:64868379-64868398 | MS.gene73527:CDS | 40.0% | |
| GATCCTAATTTGACCAATGC+GGG | - | chr4.4:64868601-64868620 | MS.gene73527:CDS | 40.0% | |
| GCTTTAACAGCTCTTGATCT+TGG | + | chr4.4:64868730-64868749 | None:intergenic | 40.0% | |
| GGATCCTAATTTGACCAATG+CGG | - | chr4.4:64868600-64868619 | MS.gene73527:CDS | 40.0% | |
| GTTGGACTCTTGAAAACATC+TGG | + | chr4.4:64868496-64868515 | None:intergenic | 40.0% | |
| CACCACCAACTCAATATCCT+TGG | + | chr4.4:64868692-64868711 | None:intergenic | 45.0% | |
| TCGTCTACATCCTCATCTTC+TGG | + | chr4.4:64868655-64868674 | None:intergenic | 45.0% | |
| TGGTATTGCTCCAGAAGATG+AGG | - | chr4.4:64868642-64868661 | MS.gene73527:CDS | 45.0% | |
| !! | ATCTTCTGGAGCAATACCAG+AGG | + | chr4.4:64868641-64868660 | None:intergenic | 45.0% |
| !! | TCCCAAGGATATTGAGTTGG+TGG | - | chr4.4:64868687-64868706 | MS.gene73527:intron | 45.0% |
| GAGCTCACAGCTACAAACTC+AGG | - | chr4.4:64868561-64868580 | MS.gene73527:CDS | 50.0% | |
| GATGAGGATGTAGACGAGAC+TGG | - | chr4.4:64868658-64868677 | MS.gene73527:intron | 50.0% | |
| GGAGCAATACCAGAGGATTC+TGG | + | chr4.4:64868634-64868653 | None:intergenic | 50.0% | |
| ! | GCACCCGCATTGGTCAAATT+AGG | + | chr4.4:64868607-64868626 | None:intergenic | 50.0% |
| CGAGACTGGTGTAGATCCCA+AGG | - | chr4.4:64868672-64868691 | MS.gene73527:intron | 55.0% | |
| TCAGGCTGCAGAGCAGTTCA+AGG | - | chr4.4:64868579-64868598 | MS.gene73527:CDS | 55.0% | |
| !! | GAGTTGGTGGTGACTCAAGC+TGG | - | chr4.4:64868700-64868719 | MS.gene73527:intron | 55.0% |
| GGTGCGAAGCCAGAATCCTC+TGG | - | chr4.4:64868622-64868641 | MS.gene73527:CDS | 60.0% | |
| !! | TTCTGGCTTCGCACCCGCAT+TGG | + | chr4.4:64868617-64868636 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 64868343 | 64868804 | 64868343 | ID=MS.gene73527 |
| chr4.4 | mRNA | 64868343 | 64868804 | 64868343 | ID=MS.gene73527.t1;Parent=MS.gene73527 |
| chr4.4 | exon | 64868787 | 64868804 | 64868787 | ID=MS.gene73527.t1.exon1;Parent=MS.gene73527.t1 |
| chr4.4 | CDS | 64868787 | 64868804 | 64868787 | ID=cds.MS.gene73527.t1;Parent=MS.gene73527.t1 |
| chr4.4 | exon | 64868343 | 64868675 | 64868343 | ID=MS.gene73527.t1.exon2;Parent=MS.gene73527.t1 |
| chr4.4 | CDS | 64868343 | 64868675 | 64868343 | ID=cds.MS.gene73527.t1;Parent=MS.gene73527.t1 |
| Gene Sequence |
| Protein sequence |
