AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053752

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053752.t1 MTR_5g059260 99.151 589 5 0 1 589 1 589 0.0 1215
MS.gene053752.t1 MTR_1g031590 55.121 537 215 6 26 559 33 546 0.0 597
MS.gene053752.t1 MTR_4g084570 57.490 494 198 4 68 559 64 547 0.0 597
MS.gene053752.t1 MTR_3g005340 55.621 507 207 5 72 569 57 554 0.0 567
MS.gene053752.t1 MTR_5g032100 41.080 426 214 11 159 559 88 501 1.15e-94 299
MS.gene053752.t1 MTR_2g016470 39.294 453 237 10 139 561 172 616 2.75e-94 301
MS.gene053752.t1 MTR_3g100310 40.141 426 218 10 159 559 102 515 2.38e-92 293
MS.gene053752.t1 MTR_3g086570 38.228 429 245 10 139 559 131 547 1.12e-91 293
MS.gene053752.t1 MTR_4g100810 44.101 356 175 7 158 509 109 444 2.02e-90 289
MS.gene053752.t1 MTR_5g099010 43.288 365 189 6 160 523 122 469 2.85e-88 285
MS.gene053752.t1 MTR_5g064620 37.986 437 238 9 140 562 97 514 5.04e-88 282
MS.gene053752.t1 MTR_3g115220 41.333 375 204 5 141 515 95 453 7.94e-88 283
MS.gene053752.t1 MTR_5g012770 43.889 360 159 10 158 509 125 449 1.10e-85 276
MS.gene053752.t1 MTR_3g099480 36.674 439 240 12 140 563 126 541 2.66e-83 271
MS.gene053752.t1 MTR_6g016015 38.849 417 226 12 158 564 116 513 9.92e-83 268
MS.gene053752.t1 MTR_4g110030 38.795 415 225 10 158 563 117 511 1.65e-81 265
MS.gene053752.t1 MTR_3g096020 38.313 415 227 7 157 559 189 586 2.53e-79 261
MS.gene053752.t1 MTR_5g042200 37.379 412 228 10 158 559 86 477 3.95e-78 256
MS.gene053752.t1 MTR_2g059350 36.501 463 245 14 112 563 86 510 7.14e-78 256
MS.gene053752.t1 MTR_4g082310 36.893 412 230 10 158 559 98 489 1.43e-77 254
MS.gene053752.t1 MTR_4g132590 37.389 452 240 9 140 559 52 492 7.43e-77 252
MS.gene053752.t1 MTR_4g125460 37.352 423 235 12 146 559 77 478 3.72e-76 250
MS.gene053752.t1 MTR_4g132590 37.389 452 240 9 140 559 151 591 7.90e-76 252
MS.gene053752.t1 MTR_4g125460 37.352 423 235 12 146 559 216 617 8.11e-75 250
MS.gene053752.t1 MTR_5g081690 36.000 425 237 12 158 571 122 522 9.43e-75 248
MS.gene053752.t1 MTR_4g019210 35.349 430 243 15 158 575 233 639 6.27e-71 240
MS.gene053752.t1 MTR_3g086570 38.361 305 175 6 139 435 131 430 1.65e-66 223
MS.gene053752.t1 MTR_3g026650 33.333 423 261 6 139 559 153 556 6.30e-66 225
MS.gene053752.t1 MTR_1g102030 34.360 422 229 14 158 559 89 482 9.17e-66 224
MS.gene053752.t1 MTR_4g023030 32.000 450 276 9 116 559 191 616 4.70e-62 216
MS.gene053752.t1 MTR_3g026650 34.294 347 205 5 139 479 153 482 3.85e-53 189
MS.gene053752.t1 MTR_4g010990 31.989 372 210 10 128 489 48 386 2.68e-47 172
MS.gene053752.t1 MTR_1g101990 34.667 300 167 11 267 559 1 278 4.51e-44 159
MS.gene053752.t1 MTR_4g010990 31.366 322 190 7 173 489 2 297 2.52e-40 150
MS.gene053752.t1 MTR_7g017330 29.024 379 234 13 107 470 55 413 2.31e-34 138
MS.gene053752.t1 MTR_3g092590 32.566 304 189 6 158 452 91 387 2.02e-33 135
MS.gene053752.t1 MTR_3g092590 32.459 305 188 7 158 452 91 387 4.26e-33 134
MS.gene053752.t1 MTR_3g026650 31.439 264 158 5 139 396 153 399 2.57e-29 120
MS.gene053752.t1 MTR_1g007390 30.945 307 188 8 158 448 91 389 2.74e-27 117
MS.gene053752.t1 MTR_4g008200 27.463 335 195 8 158 487 36 327 1.10e-25 109
MS.gene053752.t1 MTR_4g008200 27.463 335 195 8 158 487 87 378 2.44e-25 109
MS.gene053752.t1 MTR_7g064210 34.500 200 104 4 278 466 27 210 2.54e-24 106
MS.gene053752.t1 MTR_2g038320 26.330 376 222 11 132 502 70 395 8.12e-24 104
MS.gene053752.t1 MTR_1g101985 53.333 75 35 0 158 232 89 163 2.03e-18 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053752.t1 AT1G38131 69.028 494 146 2 72 560 67 558 0.0 719
MS.gene053752.t1 AT1G38065 71.663 427 116 1 139 560 3 429 0.0 654
MS.gene053752.t1 AT1G38065 74.194 403 104 0 158 560 37 439 0.0 650
MS.gene053752.t1 AT2G01480 52.441 553 232 7 24 557 9 549 0.0 584
MS.gene053752.t1 AT1G35510 54.898 541 205 10 46 559 28 556 0.0 574
MS.gene053752.t1 AT1G14970 52.651 547 237 5 24 559 17 552 0.0 570
MS.gene053752.t1 AT2G01480 53.398 515 214 6 24 519 9 516 0.0 557
MS.gene053752.t1 AT1G14970 58.581 437 171 2 123 559 6 432 0.0 540
MS.gene053752.t1 AT1G14970 60.688 407 155 1 153 559 5 406 0.0 529
MS.gene053752.t1 AT4G38390 42.448 384 205 6 141 523 107 475 3.74e-96 305
MS.gene053752.t1 AT4G24530 39.686 446 229 12 139 559 86 516 6.38e-96 303
MS.gene053752.t1 AT5G65470 39.232 469 230 14 124 559 56 502 4.56e-95 300
MS.gene053752.t1 AT1G29200 41.860 430 215 9 158 559 71 493 9.73e-93 294
MS.gene053752.t1 AT1G29200 41.860 430 215 9 158 559 71 493 9.73e-93 294
MS.gene053752.t1 AT1G29200 41.860 430 215 9 158 559 187 609 1.62e-91 294
MS.gene053752.t1 AT1G29200 41.784 426 213 9 162 559 1 419 4.28e-91 287
MS.gene053752.t1 AT3G26370 37.296 429 249 9 139 559 139 555 4.91e-90 289
MS.gene053752.t1 AT1G11990 39.083 458 230 12 123 559 159 588 6.94e-87 281
MS.gene053752.t1 AT4G16650 44.385 374 177 9 158 523 123 473 9.19e-87 280
MS.gene053752.t1 AT4G16650 44.385 374 177 9 158 523 123 473 9.19e-87 280
MS.gene053752.t1 AT1G22460 39.618 419 218 11 159 562 162 560 2.38e-86 279
MS.gene053752.t1 AT1G62330 37.199 457 243 11 139 562 207 652 2.98e-85 279
MS.gene053752.t1 AT1G20550 42.297 357 186 6 160 513 112 451 5.63e-85 276
MS.gene053752.t1 AT1G76270 40.426 376 204 6 141 513 90 448 1.24e-84 275
MS.gene053752.t1 AT1G11990 36.546 498 238 13 116 559 137 610 1.32e-83 273
MS.gene053752.t1 AT1G14020 37.621 412 227 10 158 559 92 483 1.33e-81 265
MS.gene053752.t1 AT5G64600 37.443 438 230 13 140 559 103 514 6.89e-81 264
MS.gene053752.t1 AT2G03280 38.503 374 208 8 158 525 89 446 1.04e-76 251
MS.gene053752.t1 AT5G15740 36.983 411 234 11 158 561 107 499 3.20e-74 246
MS.gene053752.t1 AT5G01100 35.731 417 228 12 158 559 217 608 1.01e-73 248
MS.gene053752.t1 AT3G02250 37.500 368 209 8 158 519 107 459 1.96e-72 241
MS.gene053752.t1 AT3G54100 35.749 414 229 13 158 559 223 611 5.67e-72 243
MS.gene053752.t1 AT1G04910 36.277 419 217 12 158 559 89 474 6.02e-72 240
MS.gene053752.t1 AT5G35570 34.607 471 263 13 114 568 211 652 2.03e-71 242
MS.gene053752.t1 AT2G37980 36.253 411 231 12 158 559 227 615 2.65e-71 241
MS.gene053752.t1 AT2G03280 35.910 401 208 9 158 525 89 473 1.03e-70 236
MS.gene053752.t1 AT5G63390 34.279 423 258 6 139 559 144 548 1.39e-70 238
MS.gene053752.t1 AT2G44500 33.258 445 272 10 119 559 138 561 1.13e-63 219
MS.gene053752.t1 AT3G07900 33.251 406 245 7 160 561 191 574 1.60e-62 216
MS.gene053752.t1 AT3G26370 36.929 241 140 5 139 372 139 374 1.90e-47 171
MS.gene053752.t1 AT1G52630 30.707 368 222 10 126 489 45 383 1.27e-45 167
MS.gene053752.t1 AT1G52630 30.218 321 193 8 173 489 2 295 3.66e-38 144
MS.gene053752.t1 AT3G03810 30.189 371 230 11 109 464 42 398 4.75e-36 143
MS.gene053752.t1 AT3G03810 30.189 371 230 11 109 464 42 398 4.75e-36 143
MS.gene053752.t1 AT3G30300 31.529 314 187 11 158 456 101 401 2.72e-32 132
MS.gene053752.t1 AT3G30300 31.529 314 187 11 158 456 101 401 2.72e-32 132
MS.gene053752.t1 AT2G44500 33.206 262 157 7 139 396 157 404 1.43e-28 119
MS.gene053752.t1 AT1G51630 26.404 356 209 9 137 487 75 382 3.67e-24 105
MS.gene053752.t1 AT3G21190 26.647 334 196 8 159 487 92 381 1.36e-22 101

Find 145 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGGAAACATCTTTATTAA+AGG 0.215473 5.1:-45958377 None:intergenic
GATTTGCATCATGAACATTT+TGG 0.253855 5.1:+45955420 MS.gene053752:CDS
GGCGGTGGCGGAGGCGGAGG+TGG 0.258644 5.1:+45954592 MS.gene053752:CDS
CTCTTCCTGGTTCTCGTTAC+CGG 0.260601 5.1:+45954833 MS.gene053752:CDS
ACAACAAGTTTGCGTTTATC+AGG 0.270838 5.1:-45959175 None:intergenic
ACATCAATATATTTAGCTTC+TGG 0.271700 5.1:+45958323 MS.gene053752:CDS
ATTGGTCATACTCCTCTTCC+TGG 0.278695 5.1:+45954820 MS.gene053752:CDS
TGTAATGCTGTGGCTGTTGC+TGG 0.286549 5.1:+45956062 MS.gene053752:CDS
ATATCATGCAGAAAAGTATT+TGG 0.315152 5.1:+45958352 MS.gene053752:CDS
TTTATCAGGAATAATGGTTT+TGG 0.315630 5.1:-45959161 None:intergenic
GAATTATCAGAATCAATTTC+AGG 0.320818 5.1:-45954883 None:intergenic
TTTGCGTTTATCAGGAATAA+TGG 0.323925 5.1:-45959167 None:intergenic
GGCGGAGGCGGAGGTGGAGG+TGG 0.328837 5.1:+45954598 MS.gene053752:CDS
AGAACTTCCTGAGGCAATAA+TGG 0.334683 5.1:+45957199 MS.gene053752:CDS
GTCATACCGCAGAGCGTTGC+TGG 0.339373 5.1:-45955946 None:intergenic
TACATTTACGCAAACGGTTA+CGG 0.345273 5.1:+45954674 MS.gene053752:CDS
CATCAATATATTTAGCTTCT+GGG 0.347457 5.1:+45958324 MS.gene053752:CDS
TTTCCGTTCACGTTCTTCAT+TGG 0.354202 5.1:+45954802 MS.gene053752:CDS
TGCTGTGCATCTTCGCTTTG+AGG 0.361326 5.1:+45957659 MS.gene053752:CDS
GGTGGCGGAGGCGGAGGTGG+AGG 0.368387 5.1:+45954595 MS.gene053752:CDS
AATGGCGGTGGCGGAGGCGG+AGG 0.369247 5.1:+45954589 MS.gene053752:CDS
AGCTCCATTTGCTAATCGTT+TGG 0.369656 5.1:+45957491 MS.gene053752:CDS
CGATCTCCACCGCGGCATTC+CGG 0.376091 5.1:+45954646 MS.gene053752:CDS
GCCTCCGCCACCGCCATTCT+CGG 0.378440 5.1:-45954584 None:intergenic
CTGCCGGTGGTTTATCTCTC+CGG 0.379278 5.1:+45954757 MS.gene053752:CDS
TTCCTCCCAATTGGCAGCAT+TGG 0.382578 5.1:+45959036 MS.gene053752:CDS
ATCTGCACTAGCTGAGAATC+TGG 0.385628 5.1:+45957590 MS.gene053752:CDS
TATCAGGAATGTCTACATTA+TGG 0.388986 5.1:-45962532 None:intergenic
TGGAGGAAAGGCAGAGAAGT+TGG 0.390688 5.1:+45957834 MS.gene053752:CDS
AGACTCCTTGGTATAAAGAT+TGG 0.391718 5.1:-45958396 None:intergenic
GAGAATGGCGGTGGCGGAGG+CGG 0.393770 5.1:+45954586 MS.gene053752:CDS
AACCATAGAGAAATCTTCTA+TGG 0.400056 5.1:-45959132 None:intergenic
TGCTGTAGACTGTAGTTATC+AGG 0.403998 5.1:-45962548 None:intergenic
ATAAAAGGAGCAAGCTCATC+TGG 0.419795 5.1:-45958428 None:intergenic
TCCTGTAGGCATCTACATTC+TGG 0.420118 5.1:-45962485 None:intergenic
GCCGAGAATGGCGGTGGCGG+AGG 0.424599 5.1:+45954583 MS.gene053752:CDS
GGCCATAGAAGATTTCTCTA+TGG 0.434460 5.1:+45959130 MS.gene053752:CDS
GACTCCTTGGTATAAAGATT+GGG 0.435088 5.1:-45958395 None:intergenic
TCAGTGTCCCCTGGCCTGAA+TGG 0.442293 5.1:+45955890 MS.gene053752:intron
GGCATCTACATTCTGGTAAA+GGG 0.445482 5.1:-45962478 None:intergenic
AAAGGCAGAGAAGTTGGAAA+TGG 0.445978 5.1:+45957840 MS.gene053752:CDS
CACTAAGCATGTCATCCATC+TGG 0.452576 5.1:-45962385 None:intergenic
TCCTCCATCATATATACAAC+AGG 0.455878 5.1:-45957818 None:intergenic
GGTGGAGATCGCGGTGGACT+CGG 0.455910 5.1:-45954634 None:intergenic
TGTTCATCCTATCTTGCAAA+AGG 0.458231 5.1:+45957301 MS.gene053752:CDS
ATGAGGGAAGTTACCACCTT+GGG 0.458726 5.1:-45959098 None:intergenic
CCATTGACTCGATAGAGGTC+AGG 0.459450 5.1:-45957922 None:intergenic
ATGAAGAACGTGAACGGAAA+CGG 0.461897 5.1:-45954799 None:intergenic
ATAGGGTTATTCCTCCCAAT+TGG 0.466456 5.1:+45959027 MS.gene053752:intron
CTATGGCCCATTAGAAAATG+AGG 0.466547 5.1:-45959115 None:intergenic
GTATAACTAGAATTGCATTC+AGG 0.467924 5.1:-45956088 None:intergenic
GCGGCCGAGAATGGCGGTGG+CGG 0.467955 5.1:+45954580 MS.gene053752:CDS
TGTCTCTCCATTATTGCCTC+AGG 0.469183 5.1:-45957206 None:intergenic
TGTAGTCCAATGCTGCCAAT+TGG 0.470944 5.1:-45959042 None:intergenic
AGAGGAAAGTTCAAAAGAAA+AGG 0.475308 5.1:+45957886 MS.gene053752:CDS
TCTATAATTTCAGTGTCCCC+TGG 0.476470 5.1:+45955881 MS.gene053752:intron
CTTCTGTATGGAAATACAAA+AGG 0.478533 5.1:+45955361 MS.gene053752:CDS
CCTGACCTCTATCGAGTCAA+TGG 0.479728 5.1:+45957922 MS.gene053752:CDS
AGGCATCTACATTCTGGTAA+AGG 0.481645 5.1:-45962479 None:intergenic
GCTCCTGTCAGTTATTACCT+TGG 0.482032 5.1:+45957275 MS.gene053752:CDS
CAAGCAAATAAATACCTCTA+GGG 0.483434 5.1:-45957962 None:intergenic
AACGGTTACGCGGCCGAGAA+TGG 0.483832 5.1:+45954571 MS.gene053752:CDS
CTAGCATCAGAGTCGAAGCT+TGG 0.488295 5.1:+45957252 MS.gene053752:CDS
TAGCATCAGAGTCGAAGCTT+GGG 0.488870 5.1:+45957253 MS.gene053752:CDS
GAGAAGAGCTCGAGGACAGA+TGG 0.496882 5.1:+45957627 MS.gene053752:CDS
TTGCAGTTGGAGTGCTCTCA+AGG 0.498262 5.1:+45962361 MS.gene053752:intron
TAGGGACCATGATTCCTTTG+CGG 0.503683 5.1:-45962415 None:intergenic
CATCCTATCTTGCAAAAGGA+AGG 0.504394 5.1:+45957305 MS.gene053752:CDS
CGGACGAAGTCACTGTCGGC+GGG 0.506458 5.1:+45954697 MS.gene053752:CDS
TTCTCCTGTTGTATATATGA+TGG 0.507992 5.1:+45957814 MS.gene053752:CDS
TACGCGGCCGAGAATGGCGG+TGG 0.508173 5.1:+45954577 MS.gene053752:CDS
CATCTTAGAAATTGTATATG+TGG 0.509769 5.1:-45957528 None:intergenic
AATGGATTCAGCTCGTGAAA+GGG 0.511300 5.1:+45957858 MS.gene053752:CDS
TCCTACACACATCAACAAAC+CGG 0.512142 5.1:-45954776 None:intergenic
TGGGATGATGCTACGTGGCA+TGG 0.512796 5.1:+45958286 MS.gene053752:CDS
ATAGAAGATTTCTCTATGGT+TGG 0.515645 5.1:+45959134 MS.gene053752:CDS
TTCACAAACCTCAATGAAAG+AGG 0.518259 5.1:-45954905 None:intergenic
TGATAATTCCTCTTTCATTG+AGG 0.520815 5.1:+45954897 MS.gene053752:CDS
AATACCTCTAGGGGAGTTAG+TGG 0.523241 5.1:-45957952 None:intergenic
GTAGTCCAATGCTGCCAATT+GGG 0.524395 5.1:-45959041 None:intergenic
GATCGAAAACCTCGTGACTC+CGG 0.529800 5.1:-45954852 None:intergenic
GGGATGATGCTACGTGGCAT+GGG 0.530350 5.1:+45958287 MS.gene053752:CDS
ACCAGAATGTAGATGCCTAC+AGG 0.530789 5.1:+45962484 MS.gene053752:CDS
TTGCTGGTTAAGACCACCAT+TGG 0.539892 5.1:-45955930 None:intergenic
AAATGGATTCAGCTCGTGAA+AGG 0.541595 5.1:+45957857 MS.gene053752:CDS
AAGTGACCGCAAAGGAATCA+TGG 0.542741 5.1:+45962409 MS.gene053752:CDS
TGGTGTTGCAAGAGACTCCT+TGG 0.543034 5.1:-45958408 None:intergenic
ATTGCCAAACGATTAGCAAA+TGG 0.544098 5.1:-45957495 None:intergenic
TTACTAATTATCAAGCATTG+AGG 0.545752 5.1:+45957553 MS.gene053752:CDS
ATTCAGCTCGTGAAAGGGGA+TGG 0.550073 5.1:+45957863 MS.gene053752:CDS
TCGAAGACACATAACGGAAA+CGG 0.564967 5.1:+45954553 MS.gene053752:CDS
CGGTAACGAGAACCAGGAAG+AGG 0.571794 5.1:-45954832 None:intergenic
ATGAAATCGAAGACACATAA+CGG 0.573992 5.1:+45954547 MS.gene053752:CDS
ACATTGTGAAATTCAAAATG+AGG 0.581885 5.1:-45956110 None:intergenic
GAGTGCTCTCAAGGACCAGA+TGG 0.582704 5.1:+45962370 MS.gene053752:CDS
CTTAGAAATTGTATATGTGG+AGG 0.583613 5.1:-45957525 None:intergenic
TGACTCCGGTAACGAGAACC+AGG 0.583762 5.1:-45954838 None:intergenic
TTGATTGTCGAGGCCAATGG+TGG 0.583847 5.1:+45955917 MS.gene053752:CDS
TATTGACAAGACTCTCCTGT+AGG 0.584214 5.1:-45962499 None:intergenic
ATGGATTCAGCTCGTGAAAG+GGG 0.592162 5.1:+45957859 MS.gene053752:CDS
CACAGTGTGCTTGTATAGTG+AGG 0.594547 5.1:+45959063 MS.gene053752:CDS
TTAGAAATTGTATATGTGGA+GGG 0.595768 5.1:-45957524 None:intergenic
GTTTCCCAATCTTTATACCA+AGG 0.595788 5.1:+45958391 MS.gene053752:CDS
TTCTCGTTACCGGAGTCACG+AGG 0.598162 5.1:+45954843 MS.gene053752:CDS
GAATGCCGCGGTGGAGATCG+CGG 0.598445 5.1:-45954643 None:intergenic
ACTCCAAGGTAATAACTGAC+AGG 0.604191 5.1:-45957278 None:intergenic
TCTGTAGATTTGTAATGCTG+TGG 0.604486 5.1:+45956052 MS.gene053752:intron
AATGAGGGAAGTTACCACCT+TGG 0.605838 5.1:-45959099 None:intergenic
TATGGCCCATTAGAAAATGA+GGG 0.607756 5.1:-45959114 None:intergenic
TTGTATATATGATGGAGGAA+AGG 0.607982 5.1:+45957822 MS.gene053752:CDS
TGTGCATCTTCGCTTTGAGG+AGG 0.608488 5.1:+45957662 MS.gene053752:CDS
ATCCTATCTTGCAAAAGGAA+GGG 0.608881 5.1:+45957306 MS.gene053752:CDS
AAGCAAATAAATACCTCTAG+GGG 0.612933 5.1:-45957961 None:intergenic
ACAATGTGTGGAAAGATCCA+AGG 0.613128 5.1:+45956126 MS.gene053752:CDS
TGTAAAACACCGGAATGCCG+CGG 0.621519 5.1:-45954655 None:intergenic
GGACGAAGTCACTGTCGGCG+GGG 0.622339 5.1:+45954698 MS.gene053752:CDS
TCCGGTTTGTTGATGTGTGT+AGG 0.625329 5.1:+45954775 MS.gene053752:CDS
GTCCAATGCTGCCAATTGGG+AGG 0.626792 5.1:-45959038 None:intergenic
TGACCAATGAAGAACGTGAA+CGG 0.630711 5.1:-45954805 None:intergenic
TCCTGTTGTATATATGATGG+AGG 0.632548 5.1:+45957817 MS.gene053752:CDS
GGTTACGCGGCCGAGAATGG+CGG 0.633753 5.1:+45954574 MS.gene053752:CDS
AAACCGGAGAGATAAACCAC+CGG 0.637048 5.1:-45954760 None:intergenic
ACGGCGGACGAAGTCACTGT+CGG 0.644805 5.1:+45954693 MS.gene053752:CDS
CTTCACATAACCATCAAGTG+TGG 0.650369 5.1:-45957168 None:intergenic
AGATAGGATGAACAACTCCA+AGG 0.653627 5.1:-45957292 None:intergenic
CTTAGTGAAAGTGACCGCAA+AGG 0.653957 5.1:+45962401 MS.gene053752:CDS
GCTCGTGAAAGGGGATGGAG+AGG 0.656809 5.1:+45957868 MS.gene053752:CDS
GCGGACGAAGTCACTGTCGG+CGG 0.657648 5.1:+45954696 MS.gene053752:CDS
AAATCAGTCAAATTCGAACA+AGG 0.661890 5.1:-45955402 None:intergenic
ATTTACGCAAACGGTTACGG+CGG 0.675625 5.1:+45954677 MS.gene053752:CDS
ATACCTCTAGGGGAGTTAGT+GGG 0.676100 5.1:-45957951 None:intergenic
TCTTAACCAGCAACGCTCTG+CGG 0.677605 5.1:+45955940 MS.gene053752:CDS
GAATGAGTGAGAAGAGCTCG+AGG 0.678527 5.1:+45957619 MS.gene053752:CDS
ATGCCCACTAACTCCCCTAG+AGG 0.680140 5.1:+45957948 MS.gene053752:CDS
TGCCGCGGTGGAGATCGCGG+TGG 0.683081 5.1:-45954640 None:intergenic
ACATAACGGAAACGGTTACG+CGG 0.684145 5.1:+45954561 MS.gene053752:CDS
ACTTGATGGTTATGTGAAGG+TGG 0.685624 5.1:+45957172 MS.gene053752:CDS
TTTGCGTAAATGTAAAACAC+CGG 0.689509 5.1:-45954665 None:intergenic
GGTGGTTAAAGAACTTCCTG+AGG 0.691083 5.1:+45957190 MS.gene053752:CDS
AAAACACCGGAATGCCGCGG+TGG 0.698901 5.1:-45954652 None:intergenic
CACACTTGATGGTTATGTGA+AGG 0.705447 5.1:+45957169 MS.gene053752:CDS
TGTCGGCGGGGATAATGACA+CGG 0.710205 5.1:+45954710 MS.gene053752:CDS
GTCCACCGCGATCTCCACCG+CGG 0.758133 5.1:+45954638 MS.gene053752:CDS
CGGCGGGGATAATGACACGG+TGG 0.779770 5.1:+45954713 MS.gene053752:CDS
TAGGTTGGGATGATGCTACG+TGG 0.784598 5.1:+45958281 MS.gene053752:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.1 gene 45954547 45962592 45954547 ID=MS.gene053752
chr5.1 mRNA 45954547 45962592 45954547 ID=MS.gene053752.t1;Parent=MS.gene053752
chr5.1 exon 45954547 45954918 45954547 ID=MS.gene053752.t1.exon1;Parent=MS.gene053752.t1
chr5.1 CDS 45954547 45954918 45954547 ID=cds.MS.gene053752.t1;Parent=MS.gene053752.t1
chr5.1 exon 45955357 45955441 45955357 ID=MS.gene053752.t1.exon2;Parent=MS.gene053752.t1
chr5.1 CDS 45955357 45955441 45955357 ID=cds.MS.gene053752.t1;Parent=MS.gene053752.t1
chr5.1 exon 45955894 45955961 45955894 ID=MS.gene053752.t1.exon3;Parent=MS.gene053752.t1
chr5.1 CDS 45955894 45955961 45955894 ID=cds.MS.gene053752.t1;Parent=MS.gene053752.t1
chr5.1 exon 45956059 45956147 45956059 ID=MS.gene053752.t1.exon4;Parent=MS.gene053752.t1
chr5.1 CDS 45956059 45956147 45956059 ID=cds.MS.gene053752.t1;Parent=MS.gene053752.t1
chr5.1 exon 45957130 45957327 45957130 ID=MS.gene053752.t1.exon5;Parent=MS.gene053752.t1
chr5.1 CDS 45957130 45957327 45957130 ID=cds.MS.gene053752.t1;Parent=MS.gene053752.t1
chr5.1 exon 45957479 45957683 45957479 ID=MS.gene053752.t1.exon6;Parent=MS.gene053752.t1
chr5.1 CDS 45957479 45957683 45957479 ID=cds.MS.gene053752.t1;Parent=MS.gene053752.t1
chr5.1 exon 45957802 45957969 45957802 ID=MS.gene053752.t1.exon7;Parent=MS.gene053752.t1
chr5.1 CDS 45957802 45957969 45957802 ID=cds.MS.gene053752.t1;Parent=MS.gene053752.t1
chr5.1 exon 45958284 45958451 45958284 ID=MS.gene053752.t1.exon8;Parent=MS.gene053752.t1
chr5.1 CDS 45958284 45958451 45958284 ID=cds.MS.gene053752.t1;Parent=MS.gene053752.t1
chr5.1 exon 45959031 45959221 45959031 ID=MS.gene053752.t1.exon9;Parent=MS.gene053752.t1
chr5.1 CDS 45959031 45959221 45959031 ID=cds.MS.gene053752.t1;Parent=MS.gene053752.t1
chr5.1 exon 45962367 45962592 45962367 ID=MS.gene053752.t1.exon10;Parent=MS.gene053752.t1
chr5.1 CDS 45962367 45962592 45962367 ID=cds.MS.gene053752.t1;Parent=MS.gene053752.t1
Gene Sequence

>MS.gene053752

ATGAAATCGAAGACACATAACGGAAACGGTTACGCGGCCGAGAATGGCGGTGGCGGAGGCGGAGGTGGAGGTGGAAGTAGTAGCTCACCGAGTCCACCGCGATCTCCACCGCGGCATTCCGGTGTTTTACATTTACGCAAACGGTTACGGCGGACGAAGTCACTGTCGGCGGGGATAATGACACGGTGGAGTCTCCGCTACTTTTTCTTACTGCCGGTGGTTTATCTCTCCGGTTTGTTGATGTGTGTAGGACCGTTTCCGTTCACGTTCTTCATTGGTCATACTCCTCTTCCTGGTTCTCGTTACCGGAGTCACGAGGTTTTCGATCATCTTTGGCCTGAAATTGATTCTGATAATTCCTCTTTCATTGAGGTTTGTGAAATTAAGCGTAATGTACAATAATCGCGTTTGCAAAGGAATAATTTTGTTTATGGATTTGGTGCTATATGAAATTTTCTTATGGAGCTGAATTCATGTTACTAATTTTGTTTGGTGAATGATTTTGGATTATTATGTAGCTGAATTCCTGTTACTAAATCTTGTTTGGTGAATAATTTTGGGTTATTATGTAGCTGAATTCGTGTTACTGAATCTTGTTTGGTGAATAATTTTGGATTTTTATGTAGCTGAATTCGTGTAACTGAATCTTGTTTGGTGAATAATTTTGTTTTTTGTGTTATTTGAAATTTTGTTGTTTTGTTGATTTTGTTGTTATTATTAACTACTTATTATAGATGCGTGATTAATAATGAAATTATTTTTGTCTATAAACTGAGTTGATTTTTAATTGTTTATTTATTTATTTTGCAGTTGTCTTCTGTATGGAAATACAAAAGGAAATTGAGAGAGAAGAAGCCTTGTTCGAATTTGACTGATTTGCATCATGAACATTTTGGTATATAATACTAAAGCTCTATGATCTATTCTCTCTTAGCTTTCGTTTCACATGTCTACCTGTTTGACTCAGACATTGGATATGATATTGACACTGACACTGATAATAATTTGAGAAAACGATATTATTCAATGTAATCATATGTGTTGGTATTGTGTTGTGTCAGACTTGCTTTCGATCAAAGATGTCGGTGCTACATAGTTTAATATTGCTGCTAATGTGTGATTTTGCTTTGTAATATAGTCAACTTTATTTAAAAGTTCTTATCAAAATAGCTGATGACTCATAAGTTGTTTTAAGCTGATTTTCATAAGCTCTGTTGTAGAAATAGTTTATATAGAATTCATGCAGAATTTGCTCCATTTCCACTTTGATCGTAACCTTATATGATAAGCTGCTAGTTAGTTATTTATGGAAAATGCTCTCTAATATTATGTTTTCTATAATTTCAGTGTCCCCTGGCCTGAATGGTTTTTTGATTGTCGAGGCCAATGGTGGTCTTAACCAGCAACGCTCTGCGGTATGACATAGTTTTTCCTTTGTCTGCCTTTTGGCAATTTTTTTATTCGACGGATGAACTATTTTTCACCGTTGTGATTTTAATTGTGGCTCTGTAGATTTGTAATGCTGTGGCTGTTGCTGGACTCCTGAATGCAATTCTAGTTATACCTCATTTTGAATTTCACAATGTGTGGAAAGATCCAAGGTGACCAATTTACCTATTTATTTTCTTTGGCTTGAGAAGTTTTTCAACACAAACATTGCTAGTTAAGTTTCATTGTTTGGGCTGTTTTTGTCAAAATATTACCTACCACATGTGGTTGGGGAGAAAATGAGTTTATCTGTTGCTTAATACAGCAGGTGTGTGTTAGCTTTATACTCTTGTTTATGTAAACTAATAATGTGCTTTGATGCAATTGTTTATGAACTGACATTGTCTTCCATCAAATGGCTATTTACAATATTTTTAAATCTAAAACAAGTTACTCACTGCTGCCTTTGTTAGGCTGATTTTTCTATCTTTCGAATTGTATAATGACGACTCTTCAGGAAAAAACTGATAGCGAATGGAGAAAGATTTTCAATTTAAGAGAGGAATTTTTTTTGCTAGGTTGTTATCATTCAATTGGAGGGATAAGGAATGAAATGGGAATAATGGATTTGAATCCTTTCCTAAGAGAACTCATATCACATGCCAAAAAGAAAAGAAGAAAATAGAATTTCATTCAATGGTTTTTAAGAAACTTTCCTACCATGTAACTCATAAAATATTTGATGTAAATATGTTTCAATAGGTTGTTTATTTAATCATTTTTCCCATAAACAGTTCCATGTCACATTGCTTTTATGTTATAAGTAGTGCATTAATTTTACTTCCTATACAAAACCTACGCTAGAGGCTGATTCTCCTACGATATACTTTTTGAATTACATTAGTTAGCACTTCTAAATTTATGGTATGTTTATGATGCGGTTGATCAAGCATTACTTGTTTAGCGTTTGTCTTCAACTAGTAGATACTGAAATCATACCTCATTTGGTGTGTGTATTTTCTCGTAGAACCATCCATACATATAATTAATTATTAAATATACCCGTAGTCAACAATACGAGGCTAATTAAACTTTTATGCCTGATTTCATTTCTGCATTCACTATCTATTTTAAATAATGTTATTTTGTGTACAGTGAATTTGGGGATATATATGATGAAGATCATTTTATATCCACACTTGATGGTTATGTGAAGGTGGTTAAAGAACTTCCTGAGGCAATAATGGAGAGACATAATTACAATATGACTAATATAACTAGCATCAGAGTCGAAGCTTGGGCTCCTGTCAGTTATTACCTTGGAGTTGTTCATCCTATCTTGCAAAAGGAAGGGTAGGTATTAGACCACTTTGCCCTCATTTCTTATTCTCTGTATTGGTTTTGGAGTGAACTTGTACTGTGAATCCATATTAGGCAGTCGACACAACTCAATTTTAAGCAAACAATTTGATGAAAGGAACTTTATTTTGGCAAACTTATGCAGGGTTATTCGAATAGCTCCATTTGCTAATCGTTTGGCAATGAGTGTCCCTCCACATATACAATTTCTAAGATGTCTTACTAATTATCAAGCATTGAGGTTTTCTTCTTCAATATCTGCACTAGCTGAGAATCTGGTTTATCGAATGAGTGAGAAGAGCTCGAGGACAGATGGAAAATATATTGCTGTGCATCTTCGCTTTGAGGAGGTACTGATGCTCATTCTATCATATTGGAAATTAGGTTTTGCTATATTCATCTCTAGGTGTCAAAAGTATACAGAATTGAAGTATTATGTAATAATTGCCTAACTATTCTAACATACAGGACATGGTAGCATTCTCCTGTTGTATATATGATGGAGGAAAGGCAGAGAAGTTGGAAATGGATTCAGCTCGTGAAAGGGGATGGAGAGGAAAGTTCAAAAGAAAAGGTCGTATCGTTGTACCTGACCTCTATCGAGTCAATGGAAAATGCCCACTAACTCCCCTAGAGGTATTTATTTGCTTGCTGATTCTTCTCATATCTTGAATTATTTTTTCCCTTAATTTAAAATCTCACCAGTCTCATGTCCTTAAGTTATTGATTTCTTAATGAGATATTGCTTTTCTTTTTTCCCTTAATCTATTCTTGATTCCAATTGATTATGGCCTCACCTTTGTGCACCGTTTTAAATTAATTTTTGTAGAAGAGCAACATATTGAGATGAAAATAAAACAAATCTATGGAAGTTTAAATTGTGATAAAACTGAGATTATATTATGGAAGAGACTGATGTTTATTTCCTGTTGATTTCCATAATTATGTAGGTTGGGATGATGCTACGTGGCATGGGATTCAATAACAATACATCAATATATTTAGCTTCTGGGAAAATATATCATGCAGAAAAGTATTTGGCTCCTTTAATAAAGATGTTTCCCAATCTTTATACCAAGGAGTCTCTTGCAACACCAGATGAGCTTGCTCCTTTTATGGTAAAAATCTTTACTGGTGAAAAAAATATATATTTCATTCTTCCATTCCATGACCTTGCTGTGATAAAATTTATAACTAATGAATTGGACTTAGTGGCATGGAGTTGTAACTTGTAACAACCCTTCATTAAAACTCTTAAATTTGGGTTGGAGTTGGACCTAGGAACCGAAGTTCTTCCATTTTAAAAGAGGATCACCAGTTTGAACTTCGATTGGTTCTTTTTTTTTTTTTTGGAATTATAGCTGATGCTCCAACACCAAGATTGCATTACTGGAAGCCAACCTGAATATTGTAATATTTATAATAATCAAGGATGTAATTAACTTCCAATTCCAAGATATAAATGATGTATTTGGCTTTTTATTGTGGTTCTCTCTTCATCCCTCATTCTAATACTTAGTATATTTGGCATGATGTTTAGTTAAAATCTTTACTGCCCTCTGTGTGACTGTGAGGTAAAATGTAAAAAAAAAAAAAAAAATCACAGGCAGGTTTAATTATTCTTTCTTTAAGTGTATGCCTTAATATTTTGTGTGACTTTCGAATATGCTCTTATGCTTAGTTTCATCCTTCTATAGGGTTATTCCTCCCAATTGGCAGCATTGGACTACACAGTGTGCTTGTATAGTGAGGTTTTTGTTACAACCCAAGGTGGTAACTTCCCTCATTTTCTAATGGGCCATAGAAGATTTCTCTATGGTTGGCATGCCAAAACCATTATTCCTGATAAACGCAAACTTGTTGTTCTTTTCGAGAACACGACTATCAGGTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATATATATATATATATATATATATATATATATATATATATATATATATATAATAGAAATATAATAAAACTTTAGGTGATATGTATCTAGGATTCATATTATAATTTCCAGCCTGTTTTATGACATGAGTGCTAACTGATTGAAATATCCCCTAGACATGAATGAGAACCTTTTGCTATGCACTAGGTAAAATTGAGAAACAGATAATTTTTTTCTATTATACCTTCTTTTAACTAAAATAGGATACAGCCAAACTCACGAGATCATTTACAAGTACTAGTTATACTCTTTATTGATTATCTACTTATCTTGAAATATCAAAATTGTTTTGAGAAAGTTGTGACCAATTAAGAAAAAGTGAAGGAGCAAAGATCTAAATCATTATTAATGGTAATCCTCTTGACGGTCCCACTTTTGGTTCCCTCTCTACTTTAATTTGCAAGACTAATCGATCTAAGAAGACCGAATTTAAGCATAGCTTTTGTATGTCTCACTCAAACAAGTTTTCGTATAGACTTGAAGCATGGCTTCCAGAAAAGGTTACTAAAATGAAAGGATCATGTACCATGAGTTGCTAACTTGGTGGTGCTCACCATAATTAACGATTAGAAACCAAAAGCTAGACCAGGCATACCTTTGTAGACATAGCCCCAGTTGGTTACTTGTAGTTTTGCAATTGTTTGTAACATGGTTTTTGCTAAACAATAACTAAAATAATATTAAGCTCAACAAATAACACAAATTTAAACAACCAAGAGAACACATACCAAATATTAGGATTCAATCATGATTCATAAATTAGGATTCATTGTTGTCCTATCCAAAGGGCTTAACAATGCTTAATAATATCCCTAAAAACAATAGCAATAATGTCAATATCCTCAAACATCAAAATAAAATATAAAAGAAGAGGGTTCATGAACAAAGCCTATTGGAACCCGAGAAAAAGTTCCCTTGCAATCACCTTGAAAGACTTAGAAATCTATCAAGAAATATCAAAGGTTGCTTGCCGAAATCATTATGAAATTGCAGTTGAAGTCGTCACTTGTCATCATCATGTCATCTATGAGTTGTTACTTCTCTGCGTTGTTAACATGTTATCAGCACGTTAGCTTGTTGTGTCATTAGGTTTTTTGGGTTTTCAAGAACTAGAGTATTATAATTTATAAGAAGAAATAGCCTTTGAAATTGAGCTTCCACATAGTTGAATGTCCGTGCAACATAAGGGGAATGCCTTGGCACATACCACCATACACATAAATCTGCATGTCTATGCTGAATGTGTCACCCCCGTGCAGGTCTCCAAAGCAACTACTTTGCATTCCAGCAGGTCTCCAAAGCAACTACTTTGCATTCCAAAGTGTAGATTTCTTCCTATCTTCATTTTAGCCCAAAATCTCCAATTTTCGTCGTTTTAACCATGTTTAAGTAATTCAATTCCACTCCTCCAAATGCCAATCTTCAGTGGTAGCTAAATTTCAAATCCAACTAAGAACACCTTGGAACCCTTAACTCGTCCAGTATTTCCTCTATAACTAGAAAGGGGGATTTGTTGGGTATTGTAACTTTGTTTAGAGGCCCTGTAATTCATACAAGATTCTCAGCCGCCACTTTTTTCTGCACTAATATCATGGGACTCGAATAATAACTTACGCTATGTCGAGTGATGCCTGCAGCTAGATGTAAACAATTCGAACTGAGCACCTAAGACTTGAATTTGGCTTGTAAAAAAAAGAGTTGCTCAAGTTTGACTCATTTATTTAAACGAGTCCAACTAGAAACTTGAGTTTGACTTGTTAAAAAAGTGGCCGTGACTTTTTTTTTTTAAAATAAATAAGCAAAGTGGGCTTGACTTGTTTTGCTATTTTATAACTTACTTTTTTGCATTTAGAATAAATTTTCTTAAATTACTTTTGAAGCTAATCAATTTAGTATTTAATAAATATTTAATATTATAAGCAAATCTACAAATAGAAATATGTGCTTAAGTAAACCCAATCTTAATTTGTATGCCCCATTCTTATATACAAGACTTTAATATTTATACATGTATATTTAGAGGGATCAAATTACACCAGTATCATATTTGGATATTGTTGCACTGTTTAATAGTTTTTTTTCCAGATACTTGTATTACAAAACTCACCGTTGGATAAGTTATTATCGTTTATATCATTTATGTAAATTCTTACGTAAATATATAATCATTTGATATGTCAATGAGATATTTACAGGTTAACATTGTTTGATAAAAGTCCATAAGTCGTTAATCTTGATGTATCCTCAGCATCTCTCCTATCAGAACCGAACAGAACAAGCTGTTCTGATCTTTGTCCTATGTCCAAGTCCCAAGTGCTTTCTCTAATTGTTTGTTGGGTTAAACATGTTTTTGGTCCCTATAAAATTGGGACCTTTTAAGTTTAGTCCTTAATTAATTTTAATAGTTCTTTTAGTCCTTTAAAAGAATTTCTGCACTCAGTATTAGTCTCTCCTCACTTCAAATTTGTTTAATTTATGTTTAAACTCTTGAGTTTTTGAACAATTTTTTGCAAACGTGTTAAGAACATTACTAAAAGTTCCTCTGCAAAAAATTGTCGCAAAATTTGATTTCTGCGTTCAGATTTTGTTAATTTTATCTTTAACTTTTGTCGTTTAAAAAAATTCATATTTAATTCGTTTCATGTTAAAAAATTCTAAATTTTAGTAAATGAACCTTTCATAGTGTTTTAAACTTGACTCAAAAAAATCGTTCAAAAATTTAAGAGTTTAAGGATAATTAAACAAACTTTGTTGTAACAGGGACTAAAATTAGAAGTAATCTTATTTTGTAGGGACTAAAAAACCTATTAAAATTAAGTAAGGACTAAACTTAGAAGTTACCAATTTTATAAGGACCAAAAACATATTTAACCCTTGTTTGTTTATGTCTGAATTAAAATTGCCAATGATTTTTATACTGGATTTGTTTCCCATCCTTGTCTGCATCTTATATTTAAGCATAGTTGCCATGTGGGTGGATTCAATAGGAAAGCTTAGGCTATGCAATCTTTGAAACACACAAGATGACACAAAATATTTGTGTATAAATTTTGCAGTTGGAGTGCTCTCAAGGACCAGATGGATGACATGCTTAGTGAAAGTGACCGCAAAGGAATCATGGTCCCTAGAGTTAGAAAAATTAACAGAAAAACTTCTGTCTACACTTACCCTTTACCAGAATGTAGATGCCTACAGGAGAGTCTTGTCAATAAAACAATTGACCATAATGTAGACATTCCTGATAACTACAGTCTACAGCAGAACAAAAGCTTAAACAGATGA
Protein sequence

>MS.gene053752.t1

MKSKTHNGNGYAAENGGGGGGGGGGSSSSPSPPRSPPRHSGVLHLRKRLRRTKSLSAGIMTRWSLRYFFLLPVVYLSGLLMCVGPFPFTFFIGHTPLPGSRYRSHEVFDHLWPEIDSDNSSFIELSSVWKYKRKLREKKPCSNLTDLHHEHFVSPGLNGFLIVEANGGLNQQRSAICNAVAVAGLLNAILVIPHFEFHNVWKDPSEFGDIYDEDHFISTLDGYVKVVKELPEAIMERHNYNMTNITSIRVEAWAPVSYYLGVVHPILQKEGVIRIAPFANRLAMSVPPHIQFLRCLTNYQALRFSSSISALAENLVYRMSEKSSRTDGKYIAVHLRFEEDMVAFSCCIYDGGKAEKLEMDSARERGWRGKFKRKGRIVVPDLYRVNGKCPLTPLEVGMMLRGMGFNNNTSIYLASGKIYHAEKYLAPLIKMFPNLYTKESLATPDELAPFMGYSSQLAALDYTVCLYSEVFVTTQGGNFPHFLMGHRRFLYGWHAKTIIPDKRKLVVLFENTTISWSALKDQMDDMLSESDRKGIMVPRVRKINRKTSVYTYPLPECRCLQESLVNKTIDHNVDIPDNYSLQQNKSLNR