AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070682

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070682.t1 MTR_5g032100 97.809 502 11 0 1 502 1 502 0.0 1028
MS.gene070682.t1 MTR_3g100310 71.757 478 135 0 26 503 40 517 0.0 734
MS.gene070682.t1 MTR_3g086570 39.278 443 237 8 70 503 130 549 5.93e-107 330
MS.gene070682.t1 MTR_3g005340 39.606 457 234 10 58 502 119 545 1.77e-101 316
MS.gene070682.t1 MTR_1g031590 38.261 460 243 13 55 503 119 548 3.00e-100 312
MS.gene070682.t1 MTR_4g084570 38.326 454 245 12 58 503 123 549 1.16e-98 308
MS.gene070682.t1 MTR_2g016470 39.293 481 262 13 44 502 143 615 6.67e-98 308
MS.gene070682.t1 MTR_5g059260 40.984 427 213 12 88 501 159 559 2.98e-93 295
MS.gene070682.t1 MTR_3g099480 31.698 530 285 11 12 502 47 538 1.72e-88 281
MS.gene070682.t1 MTR_4g082310 37.355 431 221 9 86 503 97 491 1.74e-88 280
MS.gene070682.t1 MTR_2g059350 34.115 469 249 9 55 498 71 504 1.97e-85 273
MS.gene070682.t1 MTR_5g042200 36.538 468 243 12 53 502 47 478 2.59e-85 272
MS.gene070682.t1 MTR_5g064620 33.747 483 235 9 56 503 81 513 1.34e-84 271
MS.gene070682.t1 MTR_3g086570 43.168 322 153 7 70 384 130 428 2.06e-84 268
MS.gene070682.t1 MTR_3g096020 33.849 517 271 11 12 503 118 588 3.92e-84 271
MS.gene070682.t1 MTR_4g110030 34.728 478 243 12 55 498 63 505 2.90e-83 267
MS.gene070682.t1 MTR_6g016015 35.198 429 230 9 85 500 114 507 1.69e-82 265
MS.gene070682.t1 MTR_5g099010 36.865 453 236 13 60 503 90 501 3.17e-82 266
MS.gene070682.t1 MTR_4g125460 35.076 459 245 11 59 503 61 480 1.54e-81 261
MS.gene070682.t1 MTR_4g132590 36.947 452 260 11 70 503 50 494 2.05e-81 261
MS.gene070682.t1 MTR_4g100810 36.444 450 241 14 60 502 79 490 4.95e-81 262
MS.gene070682.t1 MTR_3g115220 34.821 448 251 9 60 502 82 493 8.22e-81 262
MS.gene070682.t1 MTR_4g132590 36.947 452 260 11 70 503 149 593 3.73e-80 261
MS.gene070682.t1 MTR_4g125460 35.076 459 245 11 59 503 200 619 4.95e-80 261
MS.gene070682.t1 MTR_1g102030 34.904 467 244 14 57 503 58 484 7.11e-79 256
MS.gene070682.t1 MTR_4g019210 32.967 455 254 10 60 501 205 621 3.15e-74 247
MS.gene070682.t1 MTR_5g012770 35.604 455 227 13 60 502 95 495 6.01e-74 243
MS.gene070682.t1 MTR_3g026650 34.947 475 250 10 42 501 126 556 1.95e-73 243
MS.gene070682.t1 MTR_4g023030 34.505 455 247 10 59 501 201 616 4.33e-71 238
MS.gene070682.t1 MTR_5g081690 33.042 457 253 11 60 502 94 511 9.01e-70 232
MS.gene070682.t1 MTR_3g026650 34.321 405 210 8 42 434 126 486 2.51e-57 198
MS.gene070682.t1 MTR_1g101990 34.194 310 168 8 200 503 1 280 2.24e-48 169
MS.gene070682.t1 MTR_4g010990 27.957 465 262 14 52 502 34 439 1.54e-43 160
MS.gene070682.t1 MTR_3g026650 32.390 318 159 8 42 347 126 399 1.68e-37 142
MS.gene070682.t1 MTR_4g010990 26.471 408 234 11 102 502 2 350 3.49e-35 135
MS.gene070682.t1 MTR_7g017330 28.358 335 200 6 80 404 92 396 3.97e-30 124
MS.gene070682.t1 MTR_3g092590 28.783 337 188 8 72 392 76 376 2.08e-29 122
MS.gene070682.t1 MTR_3g092590 28.783 337 188 8 72 392 76 376 6.55e-29 121
MS.gene070682.t1 MTR_1g101985 46.226 106 56 1 57 161 58 163 1.58e-26 106
MS.gene070682.t1 MTR_7g064210 36.471 170 77 3 222 384 37 182 2.57e-24 105
MS.gene070682.t1 MTR_1g007390 27.405 343 191 9 72 392 76 382 3.68e-23 103
MS.gene070682.t1 MTR_4g008200 24.444 360 202 12 84 438 33 327 1.37e-20 94.0
MS.gene070682.t1 MTR_4g008200 24.444 360 202 12 84 438 84 378 3.19e-20 93.2
MS.gene070682.t1 MTR_2g038320 24.377 361 199 11 84 438 90 382 1.71e-19 91.3
MS.gene070682.t1 MTR_3g077710 53.125 64 11 1 291 354 4 48 1.38e-13 66.6
MS.gene070682.t1 MTR_8g017030 49.206 63 12 1 292 354 5 47 8.84e-12 61.2
MS.gene070682.t1 MTR_1g095680 39.130 92 44 2 227 318 16 95 3.48e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070682.t1 AT5G65470 73.428 493 123 3 16 502 13 503 0.0 763
MS.gene070682.t1 AT4G24530 70.650 477 140 0 26 502 41 517 0.0 726
MS.gene070682.t1 AT3G26370 38.612 461 246 9 57 503 120 557 8.56e-109 335
MS.gene070682.t1 AT1G35510 40.132 456 232 10 58 502 132 557 3.56e-103 320
MS.gene070682.t1 AT1G29200 41.150 452 240 11 70 502 50 494 2.01e-96 301
MS.gene070682.t1 AT1G29200 41.150 452 240 11 70 502 50 494 2.01e-96 301
MS.gene070682.t1 AT1G38065 39.468 451 228 12 67 501 17 438 7.93e-96 298
MS.gene070682.t1 AT1G29200 41.150 452 240 11 70 502 166 610 5.73e-95 300
MS.gene070682.t1 AT1G38065 40.278 432 213 12 86 501 26 428 6.15e-95 296
MS.gene070682.t1 AT1G38131 40.278 432 213 12 86 501 155 557 1.66e-93 296
MS.gene070682.t1 AT1G29200 41.844 423 224 10 95 502 5 420 6.34e-93 289
MS.gene070682.t1 AT2G01480 37.762 429 232 11 82 502 151 552 3.84e-91 289
MS.gene070682.t1 AT1G14970 36.784 454 252 12 57 502 7 433 3.48e-90 283
MS.gene070682.t1 AT1G14970 37.500 424 233 9 86 502 9 407 1.93e-89 280
MS.gene070682.t1 AT1G14970 36.403 467 261 13 45 502 114 553 1.23e-88 283
MS.gene070682.t1 AT1G14020 38.426 432 215 11 86 503 91 485 1.03e-87 278
MS.gene070682.t1 AT4G38390 39.329 417 216 9 89 503 126 507 2.19e-87 279
MS.gene070682.t1 AT1G62330 36.853 464 263 15 60 501 194 649 1.72e-85 277
MS.gene070682.t1 AT3G02250 34.534 472 245 12 58 500 62 498 1.39e-84 270
MS.gene070682.t1 AT1G22460 33.262 469 253 8 55 502 129 558 1.70e-84 272
MS.gene070682.t1 AT5G64600 35.699 465 241 9 59 503 90 516 2.36e-84 270
MS.gene070682.t1 AT1G04910 35.099 453 252 10 57 502 58 475 3.13e-84 270
MS.gene070682.t1 AT2G03280 37.995 429 216 11 86 500 88 480 7.17e-84 268
MS.gene070682.t1 AT1G20550 37.045 440 230 12 70 502 91 490 8.05e-84 270
MS.gene070682.t1 AT2G37980 35.371 458 245 10 58 502 197 616 1.90e-83 271
MS.gene070682.t1 AT1G11990 35.789 475 270 13 57 503 145 612 1.17e-82 268
MS.gene070682.t1 AT5G15740 33.898 472 248 13 58 500 62 498 3.79e-82 264
MS.gene070682.t1 AT1G11990 36.009 461 251 13 57 503 160 590 2.85e-81 264
MS.gene070682.t1 AT1G76270 37.204 422 222 10 85 502 105 487 3.09e-81 263
MS.gene070682.t1 AT4G16650 38.528 462 233 15 49 502 86 504 7.50e-81 262
MS.gene070682.t1 AT4G16650 38.528 462 233 15 49 502 86 504 7.50e-81 262
MS.gene070682.t1 AT2G01480 37.949 390 210 9 82 465 151 514 2.91e-80 259
MS.gene070682.t1 AT2G03280 35.746 456 216 12 86 500 88 507 8.01e-78 253
MS.gene070682.t1 AT5G35570 31.791 497 287 10 21 503 184 642 8.75e-76 251
MS.gene070682.t1 AT3G54100 33.479 457 253 9 58 501 193 611 2.00e-75 250
MS.gene070682.t1 AT2G44500 34.812 451 252 6 59 501 145 561 2.73e-74 245
MS.gene070682.t1 AT5G01100 33.405 464 254 11 56 503 186 610 5.42e-74 246
MS.gene070682.t1 AT3G07900 32.751 458 250 9 60 501 157 572 7.68e-70 234
MS.gene070682.t1 AT3G26370 43.116 276 125 7 57 323 120 372 1.39e-67 223
MS.gene070682.t1 AT5G63390 31.788 453 263 9 60 502 133 549 4.35e-64 218
MS.gene070682.t1 AT1G52630 27.655 452 259 14 59 502 45 436 2.04e-46 167
MS.gene070682.t1 AT2G44500 33.788 293 155 4 60 347 146 404 2.11e-41 154
MS.gene070682.t1 AT1G52630 26.355 406 235 12 102 502 2 348 2.00e-37 141
MS.gene070682.t1 AT3G03810 31.385 325 179 9 80 392 83 375 1.75e-34 137
MS.gene070682.t1 AT3G03810 31.385 325 179 9 80 392 83 375 1.75e-34 137
MS.gene070682.t1 AT3G30300 28.529 333 200 7 83 406 97 400 1.99e-29 122
MS.gene070682.t1 AT3G30300 28.529 333 200 7 83 406 97 400 1.99e-29 122
MS.gene070682.t1 AT3G21190 25.485 361 200 9 86 442 90 385 2.93e-23 102
MS.gene070682.t1 AT1G51630 25.613 367 192 10 86 442 91 386 4.94e-23 101

Find 124 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCATTCAGCCTGTGATTT+TGG 0.156099 5.3:-20722947 MS.gene070682:CDS
TCATTTCATTGATGTATTAA+AGG 0.217296 5.3:-20723967 MS.gene070682:CDS
TCAACTCTTCATCAGTGAAT+TGG 0.272382 5.3:+20722852 None:intergenic
TATCACAGCTTTCCGAAATA+TGG 0.272896 5.3:-20724529 MS.gene070682:CDS
CGGATTGTTGCTAGCTGCTT+TGG 0.273595 5.3:-20722792 MS.gene070682:CDS
AATCTCCAAGTGCTCTGATA+TGG 0.282135 5.3:+20723317 None:intergenic
CTTGCGATGAATCGGAAAAT+AGG 0.283776 5.3:-20724478 MS.gene070682:CDS
TTCTAGTGGTGTCCATATTT+CGG 0.293378 5.3:+20724517 None:intergenic
ATTTCGGAAAGCTGTGATAT+TGG 0.304981 5.3:+20724533 None:intergenic
AAACTCAGGCGGTGAGAAAA+TGG 0.310777 5.3:+20723409 None:intergenic
CAAAGTATAGACAGGTGATT+TGG 0.333507 5.3:-20722895 MS.gene070682:CDS
GTTTGAGCGTGCTCTGATAA+AGG 0.335059 5.3:-20722558 MS.gene070682:CDS
TGCAACAGCCAACTGGTATC+TGG 0.335170 5.3:-20723838 MS.gene070682:CDS
TACAGGTGCAGCCTTGATTC+TGG 0.351257 5.3:+20723863 None:intergenic
GGATTGTTGCTAGCTGCTTT+GGG 0.359485 5.3:-20722791 MS.gene070682:CDS
AGGAACAGGGTATGTGTATA+TGG 0.362734 5.3:+20721725 None:intergenic
AATGCATTGTGCATATTTCC+AGG 0.372472 5.3:+20722464 None:intergenic
TGACCCATCAATGCCATGTT+TGG 0.373511 5.3:+20721852 None:intergenic
CCGACCTCCTCAGGAGTCAT+TGG 0.375766 5.3:+20722812 None:intergenic
CAACTCTTCATCAGTGAATT+GGG 0.375810 5.3:+20722853 None:intergenic
AGGTAACTACTAACCTTTGT+AGG 0.380843 5.3:+20723799 None:intergenic
ACTTGAACTTGAAGACTATA+AGG 0.381120 5.3:-20721875 MS.gene070682:CDS
ATCTTCATTTCTGCTTCCCC+TGG 0.399145 5.3:-20722482 MS.gene070682:CDS
AAGGCCAAACATGGCATTGA+TGG 0.402671 5.3:-20721856 MS.gene070682:CDS
CTACATTTGTGACCTCTTGA+AGG 0.413082 5.3:+20723236 None:intergenic
TTTATATCTTGCCTCTCATA+AGG 0.413884 5.3:-20722750 MS.gene070682:intron
TGGCACATGCAATCAGGAAC+AGG 0.414607 5.3:+20721711 None:intergenic
CACCAAAATCACAGGCTGAA+TGG 0.415500 5.3:+20722945 None:intergenic
TACATCCAGGTATTTCTTGA+TGG 0.422805 5.3:-20724326 MS.gene070682:CDS
ACCAAAATCACAGGCTGAAT+GGG 0.423488 5.3:+20722946 None:intergenic
GTGCTCCACGAGTACACTTC+AGG 0.423676 5.3:+20723918 None:intergenic
TGTGACCTCTTGAAGGTAGT+TGG 0.429082 5.3:+20723243 None:intergenic
ACAGGATTTAATTCAAGGTA+TGG 0.429350 5.3:+20724132 None:intergenic
TCCACACAGGATTTAATTCA+AGG 0.432589 5.3:+20724127 None:intergenic
ATCTCCAAGTGCTCTGATAT+GGG 0.432999 5.3:+20723318 None:intergenic
TCTGCACCTCCGATTTGATA+AGG 0.437000 5.3:-20723182 MS.gene070682:intron
GTTTCTCTGCAGCGCATTCG+AGG 0.443894 5.3:-20724006 MS.gene070682:intron
TGTAAGCAATCTATTAAGAT+TGG 0.445182 5.3:+20721691 None:intergenic
TGATGAAGAGTTGAGAAGTC+AGG 0.457882 5.3:-20722843 MS.gene070682:CDS
TTGGAAATCTTGCGATGAAT+CGG 0.459724 5.3:-20724486 MS.gene070682:CDS
TATGTGTATATGGATTGTTT+AGG 0.459764 5.3:+20721735 None:intergenic
ATCCTGTGTGGAAGGATTCA+AGG 0.461236 5.3:-20724116 MS.gene070682:intron
AGCAACAATCCGACCTCCTC+AGG 0.462140 5.3:+20722803 None:intergenic
TAAGATTGGCACATGCAATC+AGG 0.462154 5.3:+20721705 None:intergenic
GACAGGTGATTTGGCAGGGA+AGG 0.464416 5.3:-20722886 MS.gene070682:CDS
TTTGAGCATGCAGTGGTTGA+AGG 0.466848 5.3:-20721795 MS.gene070682:CDS
TTTGAGCGTGCTCTGATAAA+GGG 0.467425 5.3:-20722557 MS.gene070682:CDS
CTCTTCAACTTCTATGTACT+CGG 0.468408 5.3:-20725937 MS.gene070682:intron
AGATAAATACCTTATCAAAT+CGG 0.470227 5.3:+20723173 None:intergenic
AAGTGTACTCGTGGAGCACT+AGG 0.473525 5.3:-20723914 MS.gene070682:CDS
ATGTATCCTTCAGACTTCTC+TGG 0.474788 5.3:+20724344 None:intergenic
GAAGCAATTGTTTCCTCTGA+TGG 0.475940 5.3:-20722606 MS.gene070682:CDS
CAAGTGTTGCTCCTCTGTTA+AGG 0.479005 5.3:+20725997 None:intergenic
CACGCTCAAACGGCAAAGTA+AGG 0.479789 5.3:+20722570 None:intergenic
GGTCTGAACCAGCAAAGGAT+GGG 0.485650 5.3:-20724302 MS.gene070682:CDS
AAGAGACATGATGAATGAAA+AGG 0.486074 5.3:+20725955 None:intergenic
CACTAGGGAGTACTATTCAT+TGG 0.486981 5.3:-20723898 MS.gene070682:CDS
TTGCTTCAAAGTAGAAATCC+TGG 0.492690 5.3:+20722622 None:intergenic
CGACCTCCTCAGGAGTCATT+GGG 0.492695 5.3:+20722813 None:intergenic
AGGTTTATGGTGGAGCAGCC+AGG 0.495878 5.3:-20722640 MS.gene070682:CDS
GTATAGACAGGTGATTTGGC+AGG 0.507391 5.3:-20722891 MS.gene070682:CDS
GCTGCACAGCGCTCCTCAGA+AGG 0.508238 5.3:+20724679 None:intergenic
CTGTACATGCAACAGCCAAC+TGG 0.508507 5.3:-20723845 MS.gene070682:CDS
GTTCCCCATATCAGAGCACT+TGG 0.511029 5.3:-20723322 MS.gene070682:CDS
CAGTTGGCTGTTGCATGTAC+AGG 0.511252 5.3:+20723846 None:intergenic
GTCTGAACCAGCAAAGGATG+GGG 0.514933 5.3:-20724301 MS.gene070682:CDS
TTTATCAGAGCACGCTCAAA+CGG 0.515212 5.3:+20722560 None:intergenic
GGCTCTTGCAAAGTATAGAC+AGG 0.518636 5.3:-20722903 MS.gene070682:CDS
AGGTCTGAACCAGCAAAGGA+TGG 0.519610 5.3:-20724303 MS.gene070682:CDS
TGACTTTACAACGCAGGTGT+TGG 0.521199 5.3:+20723365 None:intergenic
ACTACTAACCTTTGTAGGAC+AGG 0.531765 5.3:+20723804 None:intergenic
ACAGGTGATTTGGCAGGGAA+GGG 0.537749 5.3:-20722885 MS.gene070682:CDS
TGGAAATATGCACAATGCAT+TGG 0.538924 5.3:-20722462 MS.gene070682:intron
TGAATTAAATCCTGTGTGGA+AGG 0.541914 5.3:-20724124 MS.gene070682:CDS
CAGGATTTAATTCAAGGTAT+GGG 0.544888 5.3:+20724133 None:intergenic
ATGCATTGTGCATATTTCCA+GGG 0.554046 5.3:+20722465 None:intergenic
CACCACTAGAAAATCAAGGT+TGG 0.561009 5.3:-20724505 MS.gene070682:CDS
CGCAGGTGTTGGATATCCAT+AGG 0.561306 5.3:+20723376 None:intergenic
AGCGTTGAGGATTGCGTAGG+CGG 0.563678 5.3:+20723284 None:intergenic
CTCAAACTCTGACCACTCCA+TGG 0.563819 5.3:+20721812 None:intergenic
AGGCCAAACATGGCATTGAT+GGG 0.568191 5.3:-20721855 MS.gene070682:CDS
CTCAGAAGGCGTGAATGTCT+AGG 0.571203 5.3:+20724693 None:intergenic
TATTCGAGATACCAGAATCA+AGG 0.571849 5.3:-20723874 MS.gene070682:CDS
GAAGTCTGAAGGATACATCC+AGG 0.574141 5.3:-20724339 MS.gene070682:CDS
TACCTTGAATCCTTCCACAC+AGG 0.577185 5.3:+20724114 None:intergenic
ATGGAGGTCTGAACCAGCAA+AGG 0.578859 5.3:-20724307 MS.gene070682:CDS
TGGACACCACTAGAAAATCA+AGG 0.585064 5.3:-20724509 MS.gene070682:CDS
AAAGTAGACAAGGTCAACTC+AGG 0.585115 5.3:-20721767 MS.gene070682:CDS
ATCCAGGTATTTCTTGATGG+AGG 0.585774 5.3:-20724323 MS.gene070682:CDS
CTAGACATTCACGCCTTCTG+AGG 0.586628 5.3:-20724692 MS.gene070682:CDS
AGCTTCCTGAAGTGTACTCG+TGG 0.588164 5.3:-20723923 MS.gene070682:CDS
GTTAAGGAAGCTAAGCTCCA+TGG 0.590912 5.3:+20726013 None:intergenic
ATTCGAGGATATATTTGATG+TGG 0.596280 5.3:-20723991 MS.gene070682:CDS
GAACTCCAACTACCTTCAAG+AGG 0.598101 5.3:-20723248 MS.gene070682:CDS
GATGAAGAGTTGAGAAGTCA+GGG 0.598917 5.3:-20722842 MS.gene070682:CDS
GACCTCCATCAAGAAATACC+TGG 0.602514 5.3:+20724321 None:intergenic
TAGGCAAGTTATCAAAACTC+AGG 0.602743 5.3:+20723395 None:intergenic
GCATTACCAGAGAAGTCTGA+AGG 0.606748 5.3:-20724350 MS.gene070682:CDS
TATAGACAGGTGATTTGGCA+GGG 0.613330 5.3:-20722890 MS.gene070682:CDS
AGTGTACTCGTGGAGCACTA+GGG 0.613717 5.3:-20723913 MS.gene070682:CDS
AAAATCACAGGCTGAATGGG+CGG 0.625647 5.3:+20722949 None:intergenic
TGCATTGTGCATATTTCCAG+GGG 0.626107 5.3:+20722466 None:intergenic
TAAATACCTTATCAAATCGG+AGG 0.627743 5.3:+20723176 None:intergenic
TCTCCAAGTGCTCTGATATG+GGG 0.632940 5.3:+20723319 None:intergenic
GAGCTTAGCTTCCTTAACAG+AGG 0.634652 5.3:-20726008 MS.gene070682:CDS
GAAGACTATAAGGCCAAACA+TGG 0.637671 5.3:-20721865 MS.gene070682:CDS
ATTTCTTCAGACGAGCGTTG+AGG 0.639996 5.3:+20723271 None:intergenic
ACTGTTCTTGAACAAAACCA+TGG 0.641651 5.3:-20721829 MS.gene070682:CDS
CCAATGACTCCTGAGGAGGT+CGG 0.645787 5.3:-20722812 MS.gene070682:CDS
ACATGGGTAAACCTTATGAG+AGG 0.649028 5.3:+20722739 None:intergenic
GTTGATGATAGGTTGGACAC+AGG 0.651170 5.3:-20721902 MS.gene070682:intron
GTCAGAGTTTGAGCATGCAG+TGG 0.658254 5.3:-20721802 MS.gene070682:CDS
ACGAGCGTTGAGGATTGCGT+AGG 0.660209 5.3:+20723281 None:intergenic
GGCACATGCAATCAGGAACA+GGG 0.662155 5.3:+20721712 None:intergenic
ATGCCCAATGACTCCTGAGG+AGG 0.666537 5.3:-20722816 MS.gene070682:CDS
ATGATGAATGAAAAGGAACA+AGG 0.675260 5.3:+20725962 None:intergenic
GAAAGTTGACTTTACAACGC+AGG 0.676645 5.3:+20723359 None:intergenic
ACCTTGAATTAAATCCTGTG+TGG 0.679224 5.3:-20724128 MS.gene070682:CDS
GAAGGTCATGAAAGTAGACA+AGG 0.680741 5.3:-20721777 MS.gene070682:CDS
TCTGAACCAGCAAAGGATGG+GGG 0.685694 5.3:-20724300 MS.gene070682:intron
GCAAGTTATCAAAACTCAGG+CGG 0.692975 5.3:+20723398 None:intergenic
TCTTGAACAAAACCATGGAG+TGG 0.694603 5.3:-20721824 MS.gene070682:CDS
ACGATGCCCAATGACTCCTG+AGG 0.696187 5.3:-20722819 MS.gene070682:CDS
ACTAATATAGTAGTCAAGTG+CGG 0.713873 5.3:+20722517 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAATAAAATTGAAG+GGG - chr5.3:20722418-20722437 MS.gene070682:intron 10.0%
!! GAAAATAAAATAAAATTGAA+GGG - chr5.3:20722417-20722436 MS.gene070682:intron 10.0%
!!! AAAGATTTATAATTTTTACA+CGG - chr5.3:20723490-20723509 MS.gene070682:intron 10.0%
!!! TTTAATTCTTTTTCTAATTT+GGG - chr5.3:20721848-20721867 MS.gene070682:CDS 10.0%
!!! TTTTAATTCTTTTTCTAATT+TGG - chr5.3:20721847-20721866 MS.gene070682:CDS 10.0%
!! AAATGAAATAAAAGACATAA+CGG + chr5.3:20721890-20721909 None:intergenic 15.0%
!! GGAAAATAAAATAAAATTGA+AGG - chr5.3:20722416-20722435 MS.gene070682:intron 15.0%
!! TAAATAATTGAATATGAACA+TGG + chr5.3:20725389-20725408 None:intergenic 15.0%
!! TATCTTGAAATTTATATGAT+TGG - chr5.3:20722722-20722741 MS.gene070682:intron 15.0%
!! TTCAAACTAAAAAATAACAT+GGG + chr5.3:20724995-20725014 None:intergenic 15.0%
!!! ATCGATTTTAAATAAAGATA+TGG - chr5.3:20724165-20724184 MS.gene070682:CDS 15.0%
!!! ATTTTGAAATTCTAATATCA+TGG - chr5.3:20724243-20724262 MS.gene070682:intron 15.0%
!!! TAATATTTTATTACAGGATA+TGG - chr5.3:20724743-20724762 MS.gene070682:intron 15.0%
!!! TCTTAATTTTTCTTTTTTGT+TGG - chr5.3:20724628-20724647 MS.gene070682:intron 15.0%
!!! TCTTTAAACAACTTTTATTT+TGG - chr5.3:20723093-20723112 MS.gene070682:intron 15.0%
!!! TGTTTTTTTTTTTTGTTAGT+TGG - chr5.3:20721971-20721990 MS.gene070682:intron 15.0%
!!! TTAAGATTAATTTTTGGATT+GGG - chr5.3:20722163-20722182 MS.gene070682:intron 15.0%
!! AAAAAAAATGATATGATGCT+TGG - chr5.3:20722528-20722547 MS.gene070682:CDS 20.0%
!! AAAAGCTTGATCTAATTAAA+AGG + chr5.3:20723455-20723474 None:intergenic 20.0%
!! AAAAGGGAATTAACAAAAAA+AGG - chr5.3:20722387-20722406 MS.gene070682:intron 20.0%
!! AAAGGGAATTAACAAAAAAA+GGG - chr5.3:20722388-20722407 MS.gene070682:intron 20.0%
!! ACAATACTCTAAATATGATA+CGG + chr5.3:20725498-20725517 None:intergenic 20.0%
!! AGATAAATACCTTATCAAAT+CGG + chr5.3:20724545-20724564 None:intergenic 20.0%
!! ATGAAATGTAGTCATATATA+TGG + chr5.3:20725299-20725318 None:intergenic 20.0%
!! ATTAACAAAAAAAGGGAATT+AGG - chr5.3:20722395-20722414 MS.gene070682:intron 20.0%
!! CTTCAAACTAAAAAATAACA+TGG + chr5.3:20724996-20725015 None:intergenic 20.0%
!! TAAGTTGTTTATATCTAAAC+AGG + chr5.3:20722583-20722602 None:intergenic 20.0%
!! TAGATGATAATAAAAATGCA+GGG - chr5.3:20724499-20724518 MS.gene070682:CDS 20.0%
!! TATTATAAAATTGTGTCCAA+TGG - chr5.3:20722466-20722485 MS.gene070682:CDS 20.0%
!! TATTTATTATGAGATGCATT+TGG - chr5.3:20725403-20725422 MS.gene070682:intron 20.0%
!! TCATATATATGGTAAATCTA+GGG + chr5.3:20725288-20725307 None:intergenic 20.0%
!! TCATTTCATTGATGTATTAA+AGG - chr5.3:20723748-20723767 MS.gene070682:intron 20.0%
!! TTTAGAGAAATTTAGAAACT+GGG - chr5.3:20722041-20722060 MS.gene070682:intron 20.0%
!!! AAAGGTATAATGGAATTAAT+TGG - chr5.3:20722224-20722243 MS.gene070682:intron 20.0%
!!! AAGGTATAATGGAATTAATT+GGG - chr5.3:20722225-20722244 MS.gene070682:intron 20.0%
!!! ATTTTAAGGTGTATATTATG+GGG - chr5.3:20724191-20724210 MS.gene070682:intron 20.0%
!!! CTTCAATTTTTTTTCTTTCT+CGG - chr5.3:20721815-20721834 MS.gene070682:CDS 20.0%
!!! GATTTTAAGGTGTATATTAT+GGG - chr5.3:20724190-20724209 MS.gene070682:intron 20.0%
!!! GTGTGATAATATTTTATTAC+AGG - chr5.3:20724737-20724756 MS.gene070682:intron 20.0%
!!! GTTAAGATTAATTTTTGGAT+TGG - chr5.3:20722162-20722181 MS.gene070682:intron 20.0%
!!! TCGTTATGATTTTAGTTTAT+TGG - chr5.3:20722943-20722962 MS.gene070682:CDS 20.0%
!!! TGATTTTAAGGTGTATATTA+TGG - chr5.3:20724189-20724208 MS.gene070682:intron 20.0%
!!! TGCAATTAATTTGAAGAAAA+GGG - chr5.3:20722371-20722390 MS.gene070682:intron 20.0%
!!! TTAGCGTTAAGATTAATTTT+TGG - chr5.3:20722157-20722176 MS.gene070682:intron 20.0%
!!! TTGCAATTAATTTGAAGAAA+AGG - chr5.3:20722370-20722389 MS.gene070682:intron 20.0%
!!! TTTTAGAGAAATTTAGAAAC+TGG - chr5.3:20722040-20722059 MS.gene070682:intron 20.0%
! AATTATAGATCACATGGTTT+AGG - chr5.3:20722293-20722312 MS.gene070682:intron 25.0%
! ACAACTTAATTAAACGCTTA+TGG - chr5.3:20722595-20722614 MS.gene070682:CDS 25.0%
! AGACAAATGCATGTAAAAAT+AGG + chr5.3:20724700-20724719 None:intergenic 25.0%
! ATTCATGACTTGAAATGTTT+CGG - chr5.3:20723972-20723991 MS.gene070682:CDS 25.0%
! CACACATATAAAATAATCCA+AGG + chr5.3:20721929-20721948 None:intergenic 25.0%
! CATATATATGGTAAATCTAG+GGG + chr5.3:20725287-20725306 None:intergenic 25.0%
! CCATGTGATCTATAATTAAT+AGG + chr5.3:20722290-20722309 None:intergenic 25.0%
! CCTATTAATTATAGATCACA+TGG - chr5.3:20722287-20722306 MS.gene070682:intron 25.0%
! GACAAATGCATGTAAAAATA+GGG + chr5.3:20724699-20724718 None:intergenic 25.0%
! GTAGATGATAATAAAAATGC+AGG - chr5.3:20724498-20724517 MS.gene070682:CDS 25.0%
! GTCATATATATGGTAAATCT+AGG + chr5.3:20725289-20725308 None:intergenic 25.0%
! TAATAGATATTGAGCATGTT+TGG + chr5.3:20722854-20722873 None:intergenic 25.0%
! TCGACTAATGTTAAAGTTAT+TGG + chr5.3:20723999-20724018 None:intergenic 25.0%
! TCTAGACAACATATTTATGA+TGG - chr5.3:20725734-20725753 MS.gene070682:intron 25.0%
! TTAGAGAAATTTAGAAACTG+GGG - chr5.3:20722042-20722061 MS.gene070682:intron 25.0%
!! AATTCTTTTTCTAATTTGGG+TGG - chr5.3:20721851-20721870 MS.gene070682:CDS 25.0%
!! ATTCTAATATCATGGTGTTT+TGG - chr5.3:20724251-20724270 MS.gene070682:intron 25.0%
!! CAATATTTGTGTGTGTTTTT+GGG - chr5.3:20722075-20722094 MS.gene070682:intron 25.0%
!! TATGTGTATATGGATTGTTT+AGG + chr5.3:20725983-20726002 None:intergenic 25.0%
!! TCAATATTTGTGTGTGTTTT+TGG - chr5.3:20722074-20722093 MS.gene070682:intron 25.0%
!! TCTAGACTAGTTACTAATAT+TGG + chr5.3:20725330-20725349 None:intergenic 25.0%
!! TTATCTAATCATTTTGTCAC+TGG - chr5.3:20723326-20723345 MS.gene070682:CDS 25.0%
!!! AAGGGAAGATATTTTAGATA+AGG + chr5.3:20725051-20725070 None:intergenic 25.0%
!!! AATATTTGTGTGTGTTTTTG+GGG - chr5.3:20722076-20722095 MS.gene070682:intron 25.0%
!!! TAAAGATATGGCTGATTTTA+AGG - chr5.3:20724177-20724196 MS.gene070682:CDS 25.0%
!!! TATTGTATTATTTTGTTCCG+TGG - chr5.3:20723648-20723667 MS.gene070682:intron 25.0%
!!! TTCAGTAATGTTTTTTTCCT+TGG - chr5.3:20721909-20721928 MS.gene070682:intron 25.0%
AAAATCATAACGAGATGCTA+AGG + chr5.3:20722936-20722955 None:intergenic 30.0%
AAAGCAAGTTCCAAGTTATA+AGG + chr5.3:20722978-20722997 None:intergenic 30.0%
AAAGTACAGAATACAGATTC+AGG + chr5.3:20723939-20723958 None:intergenic 30.0%
AACAGTCTCATAAGTACTTA+TGG - chr5.3:20722802-20722821 MS.gene070682:CDS 30.0%
AAGAGACATGATGAATGAAA+AGG + chr5.3:20721763-20721782 None:intergenic 30.0%
AAGTACTTATGAGACTGTTT+GGG + chr5.3:20722801-20722820 None:intergenic 30.0%
AAGTTCCAAGTTATAAGGTA+TGG + chr5.3:20722973-20722992 None:intergenic 30.0%
ACAGGATTTAATTCAAGGTA+TGG + chr5.3:20723586-20723605 None:intergenic 30.0%
ACTAATATAGTAGTCAAGTG+CGG + chr5.3:20725201-20725220 None:intergenic 30.0%
ACTTGAACTTGAAGACTATA+AGG - chr5.3:20725840-20725859 MS.gene070682:intron 30.0%
ATAAAATTGTGTCCAATGGA+AGG - chr5.3:20722470-20722489 MS.gene070682:CDS 30.0%
ATACTCTAAATATGATACGG+AGG + chr5.3:20725495-20725514 None:intergenic 30.0%
ATATTTATGATGGTACTGCA+AGG - chr5.3:20725744-20725763 MS.gene070682:intron 30.0%
ATCAATATTAAGCCTTCCAT+TGG + chr5.3:20722485-20722504 None:intergenic 30.0%
ATGACACTTAATACATGAGA+TGG - chr5.3:20725674-20725693 MS.gene070682:intron 30.0%
ATGATGAATGAAAAGGAACA+AGG + chr5.3:20721756-20721775 None:intergenic 30.0%
ATTCGAGGATATATTTGATG+TGG - chr5.3:20723724-20723743 MS.gene070682:intron 30.0%
CAGGATTTAATTCAAGGTAT+GGG + chr5.3:20723585-20723604 None:intergenic 30.0%
TAAAGTAACTTTCACTCACT+TGG + chr5.3:20723263-20723282 None:intergenic 30.0%
TAAATACCTTATCAAATCGG+AGG + chr5.3:20724542-20724561 None:intergenic 30.0%
TAAGTACTTATGAGACTGTT+TGG + chr5.3:20722802-20722821 None:intergenic 30.0%
TACTCTAAATATGATACGGA+GGG + chr5.3:20725494-20725513 None:intergenic 30.0%
TATAACTATCTTGACGTTAC+AGG - chr5.3:20724651-20724670 MS.gene070682:intron 30.0%
TCAAGATAGCTTATGAAAAC+AGG + chr5.3:20722710-20722729 None:intergenic 30.0%
TGGGGTGAAAGAAATTATAT+GGG - chr5.3:20722094-20722113 MS.gene070682:intron 30.0%
TTGGGGTGAAAGAAATTATA+TGG - chr5.3:20722093-20722112 MS.gene070682:intron 30.0%
TTTATATCTTGCCTCTCATA+AGG - chr5.3:20724965-20724984 MS.gene070682:intron 30.0%
! CTAGTCTAGAATACTGATAA+TGG - chr5.3:20725340-20725359 MS.gene070682:intron 30.0%
! GGAATTATGTGACTTTTACA+AGG + chr5.3:20722562-20722581 None:intergenic 30.0%
! TAAAATATCTTCCCTTTTCC+AGG - chr5.3:20725055-20725074 MS.gene070682:intron 30.0%
!! ATGAATGCTTTGTTGATGAT+AGG - chr5.3:20725802-20725821 MS.gene070682:intron 30.0%
AAAATTGAAGGGGCAGAATT+AGG - chr5.3:20722428-20722447 MS.gene070682:intron 35.0%
AAACAGAATTCCTGTGAACA+TGG - chr5.3:20725432-20725451 MS.gene070682:intron 35.0%
AACAGAATTCCTGTGAACAT+GGG - chr5.3:20725433-20725452 MS.gene070682:intron 35.0%
AATGCATGTAAAAATAGGGC+AGG + chr5.3:20724695-20724714 None:intergenic 35.0%
AATGCATTGTGCATATTTCC+AGG + chr5.3:20725254-20725273 None:intergenic 35.0%
ACACTTAATACATGAGATGG+AGG - chr5.3:20725677-20725696 MS.gene070682:intron 35.0%
ACCTTGAATTAAATCCTGTG+TGG - chr5.3:20723587-20723606 MS.gene070682:intron 35.0%
ACCTTGACTTAACAATGTTG+AGG - chr5.3:20725363-20725382 MS.gene070682:intron 35.0%
ACTGTTCTTGAACAAAACCA+TGG - chr5.3:20725886-20725905 MS.gene070682:intron 35.0%
AGGTAACTACTAACCTTTGT+AGG + chr5.3:20723919-20723938 None:intergenic 35.0%
ATGCATGTAAAAATAGGGCA+GGG + chr5.3:20724694-20724713 None:intergenic 35.0%
ATGCATTGTGCATATTTCCA+GGG + chr5.3:20725253-20725272 None:intergenic 35.0%
ATTTCGGAAAGCTGTGATAT+TGG + chr5.3:20723185-20723204 None:intergenic 35.0%
CAAAGTATAGACAGGTGATT+TGG - chr5.3:20724820-20724839 MS.gene070682:intron 35.0%
CAACTCTTCATCAGTGAATT+GGG + chr5.3:20724865-20724884 None:intergenic 35.0%
CACGAAAGAAAGGAAAAACT+AGG + chr5.3:20722913-20722932 None:intergenic 35.0%
CACTTAATACATGAGATGGA+GGG - chr5.3:20725678-20725697 MS.gene070682:intron 35.0%
CTCTTCAACTTCTATGTACT+CGG - chr5.3:20721778-20721797 MS.gene070682:CDS 35.0%
GAAATTTAGAAACTGGGGTT+TGG - chr5.3:20722047-20722066 MS.gene070682:intron 35.0%
GATATTGAGCATGTTTGGAT+TGG + chr5.3:20722849-20722868 None:intergenic 35.0%
GCTTTCCATACCTTATAACT+TGG - chr5.3:20722965-20722984 MS.gene070682:intron 35.0%
GGCAGAATTAGGAATTGTTT+AGG - chr5.3:20722439-20722458 MS.gene070682:intron 35.0%
GGGGTGAAAGAAATTATATG+GGG - chr5.3:20722095-20722114 MS.gene070682:intron 35.0%
TACATCCAGGTATTTCTTGA+TGG - chr5.3:20723389-20723408 MS.gene070682:CDS 35.0%
TAGGCAAGTTATCAAAACTC+AGG + chr5.3:20724323-20724342 None:intergenic 35.0%
TATCACAGCTTTCCGAAATA+TGG - chr5.3:20723186-20723205 MS.gene070682:CDS 35.0%
TATTCGAGATACCAGAATCA+AGG - chr5.3:20723841-20723860 MS.gene070682:CDS 35.0%
TCAACTCTTCATCAGTGAAT+TGG + chr5.3:20724866-20724885 None:intergenic 35.0%
TCACAGGAATTCTGTTTGTA+TGG + chr5.3:20725429-20725448 None:intergenic 35.0%
TCCACACAGGATTTAATTCA+AGG + chr5.3:20723591-20723610 None:intergenic 35.0%
TGAATTAAATCCTGTGTGGA+AGG - chr5.3:20723591-20723610 MS.gene070682:intron 35.0%
TGGAAATATGCACAATGCAT+TGG - chr5.3:20725253-20725272 MS.gene070682:intron 35.0%
TGGAGGAAAAGCTGAAAAAT+TGG - chr5.3:20724791-20724810 MS.gene070682:intron 35.0%
TTCTAGTGGTGTCCATATTT+CGG + chr5.3:20723201-20723220 None:intergenic 35.0%
TTGCTTCAAAGTAGAAATCC+TGG + chr5.3:20725096-20725115 None:intergenic 35.0%
TTGGAAATCTTGCGATGAAT+CGG - chr5.3:20723229-20723248 MS.gene070682:CDS 35.0%
! AACATTTTCCAGATACCAGT+TGG + chr5.3:20723888-20723907 None:intergenic 35.0%
! ACGATGTAATGTATTGATCG+AGG - chr5.3:20723678-20723697 MS.gene070682:intron 35.0%
! ACTTGGAACTTGCTTTATGT+AGG - chr5.3:20722982-20723001 MS.gene070682:intron 35.0%
! CTTTGAGAACACATGATGTT+TGG - chr5.3:20723289-20723308 MS.gene070682:CDS 35.0%
! GGACATGCTATTACTCATTT+TGG - chr5.3:20722314-20722333 MS.gene070682:intron 35.0%
! TTCCAACCTTGATTTTCTAG+TGG + chr5.3:20723215-20723234 None:intergenic 35.0%
!! ATGCTTTGTTGATGATAGGT+TGG - chr5.3:20725806-20725825 MS.gene070682:intron 35.0%
!! CGTGAATGTCTAGGTTTTAT+AGG + chr5.3:20723016-20723035 None:intergenic 35.0%
!! GAGTTTTGATAACTTGCCTA+TGG - chr5.3:20724323-20724342 MS.gene070682:CDS 35.0%
!! GTTTTGGTATGATGCAGTTA+TGG - chr5.3:20724267-20724286 MS.gene070682:intron 35.0%
!! TATTACTCATTTTGGTGTGG+TGG - chr5.3:20722322-20722341 MS.gene070682:intron 35.0%
!! TGCTATTACTCATTTTGGTG+TGG - chr5.3:20722319-20722338 MS.gene070682:intron 35.0%
AAAGTAGACAAGGTCAACTC+AGG - chr5.3:20725948-20725967 MS.gene070682:CDS 40.0%
AATCTCCAAGTGCTCTGATA+TGG + chr5.3:20724401-20724420 None:intergenic 40.0%
ACATGGGTAAACCTTATGAG+AGG + chr5.3:20724979-20724998 None:intergenic 40.0%
ACCAAAATCACAGGCTGAAT+GGG + chr5.3:20724772-20724791 None:intergenic 40.0%
ACTACTAACCTTTGTAGGAC+AGG + chr5.3:20723914-20723933 None:intergenic 40.0%
AGGAACAGGGTATGTGTATA+TGG + chr5.3:20725993-20726012 None:intergenic 40.0%
ATCCAGGTATTTCTTGATGG+AGG - chr5.3:20723392-20723411 MS.gene070682:CDS 40.0%
ATCTCCAAGTGCTCTGATAT+GGG + chr5.3:20724400-20724419 None:intergenic 40.0%
ATGTATCCTTCAGACTTCTC+TGG + chr5.3:20723374-20723393 None:intergenic 40.0%
ATTACATCGTGCACAATCCA+CGG + chr5.3:20723668-20723687 None:intergenic 40.0%
CACCACTAGAAAATCAAGGT+TGG - chr5.3:20723210-20723229 MS.gene070682:CDS 40.0%
CATTAATGTCCCATGTTCAC+AGG + chr5.3:20725445-20725464 None:intergenic 40.0%
CTACATTTGTGACCTCTTGA+AGG + chr5.3:20724482-20724501 None:intergenic 40.0%
CTTGCGATGAATCGGAAAAT+AGG - chr5.3:20723237-20723256 MS.gene070682:CDS 40.0%
GAAGACTATAAGGCCAAACA+TGG - chr5.3:20725850-20725869 MS.gene070682:intron 40.0%
GAAGCAATTGTTTCCTCTGA+TGG - chr5.3:20725109-20725128 MS.gene070682:intron 40.0%
GAAGGTCATGAAAGTAGACA+AGG - chr5.3:20725938-20725957 MS.gene070682:CDS 40.0%
GATGAAGAGTTGAGAAGTCA+GGG - chr5.3:20724873-20724892 MS.gene070682:intron 40.0%
GCAAGTTATCAAAACTCAGG+CGG + chr5.3:20724320-20724339 None:intergenic 40.0%
GCCTCAACATTGTTAAGTCA+AGG + chr5.3:20725367-20725386 None:intergenic 40.0%
TAAGATTGGCACATGCAATC+AGG + chr5.3:20726013-20726032 None:intergenic 40.0%
TCCACCATAAACCTGGAAAA+GGG + chr5.3:20725069-20725088 None:intergenic 40.0%
TCTTGAACAAAACCATGGAG+TGG - chr5.3:20725891-20725910 MS.gene070682:intron 40.0%
TGATGAAGAGTTGAGAAGTC+AGG - chr5.3:20724872-20724891 MS.gene070682:intron 40.0%
TGCATTGTGCATATTTCCAG+GGG + chr5.3:20725252-20725271 None:intergenic 40.0%
TGGACACCACTAGAAAATCA+AGG - chr5.3:20723206-20723225 MS.gene070682:CDS 40.0%
TTCAAAACAATGTTCAGCGC+AGG + chr5.3:20724107-20724126 None:intergenic 40.0%
TTTATCAGAGCACGCTCAAA+CGG + chr5.3:20725158-20725177 None:intergenic 40.0%
TTTGAGCGTGCTCTGATAAA+GGG - chr5.3:20725158-20725177 MS.gene070682:intron 40.0%
! ATGTTTTGCCTGTCCTACAA+AGG - chr5.3:20723903-20723922 MS.gene070682:CDS 40.0%
! CGAGTGAGAGAAAGGTATAA+TGG - chr5.3:20722214-20722233 MS.gene070682:intron 40.0%
! CTCTTTTTGTCTTCCATCAG+AGG + chr5.3:20725125-20725144 None:intergenic 40.0%
! GAAAGTTGACTTTACAACGC+AGG + chr5.3:20724359-20724378 None:intergenic 40.0%
! TATAGACAGGTGATTTGGCA+GGG - chr5.3:20724825-20724844 MS.gene070682:intron 40.0%
! TCTTCCCTTTTCCAGGTTTA+TGG - chr5.3:20725062-20725081 MS.gene070682:intron 40.0%
! TTTTCCTCCACCAAAATCAC+AGG + chr5.3:20724781-20724800 None:intergenic 40.0%
!! CACTAGGGAGTACTATTCAT+TGG - chr5.3:20723817-20723836 MS.gene070682:CDS 40.0%
AAAATCACAGGCTGAATGGG+CGG + chr5.3:20724769-20724788 None:intergenic 45.0%
AAACTCAGGCGGTGAGAAAA+TGG + chr5.3:20724309-20724328 None:intergenic 45.0%
ATCCTGTGTGGAAGGATTCA+AGG - chr5.3:20723599-20723618 MS.gene070682:intron 45.0%
ATCTTCATTTCTGCTTCCCC+TGG - chr5.3:20725233-20725252 MS.gene070682:intron 45.0%
CACCAAAATCACAGGCTGAA+TGG + chr5.3:20724773-20724792 None:intergenic 45.0%
CTCCACCATAAACCTGGAAA+AGG + chr5.3:20725070-20725089 None:intergenic 45.0%
GAAAGTAGCGAGTGAGAGAA+AGG - chr5.3:20722206-20722225 MS.gene070682:intron 45.0%
GAACTCCAACTACCTTCAAG+AGG - chr5.3:20724467-20724486 MS.gene070682:intron 45.0%
GAAGTCTGAAGGATACATCC+AGG - chr5.3:20723376-20723395 MS.gene070682:CDS 45.0%
GACCTCCATCAAGAAATACC+TGG + chr5.3:20723397-20723416 None:intergenic 45.0%
GAGCTTAGCTTCCTTAACAG+AGG - chr5.3:20721707-20721726 MS.gene070682:CDS 45.0%
GATGCTAAGGCACGAAAGAA+AGG + chr5.3:20722923-20722942 None:intergenic 45.0%
GCATTACCAGAGAAGTCTGA+AGG - chr5.3:20723365-20723384 MS.gene070682:CDS 45.0%
GGCTCTTGCAAAGTATAGAC+AGG - chr5.3:20724812-20724831 MS.gene070682:intron 45.0%
GTTTGAGCGTGCTCTGATAA+AGG - chr5.3:20725157-20725176 MS.gene070682:intron 45.0%
TACCTTGAATCCTTCCACAC+AGG + chr5.3:20723604-20723623 None:intergenic 45.0%
TCTCCAAGTGCTCTGATATG+GGG + chr5.3:20724399-20724418 None:intergenic 45.0%
TGACCCATCAATGCCATGTT+TGG + chr5.3:20725866-20725885 None:intergenic 45.0%
TGACTTTACAACGCAGGTGT+TGG + chr5.3:20724353-20724372 None:intergenic 45.0%
TGGTGGTTGAGGTTCTTTGT+TGG - chr5.3:20722339-20722358 MS.gene070682:intron 45.0%
TGTGACCTCTTGAAGGTAGT+TGG + chr5.3:20724475-20724494 None:intergenic 45.0%
TTTGAGCATGCAGTGGTTGA+AGG - chr5.3:20725920-20725939 MS.gene070682:intron 45.0%
! AAGGCCAAACATGGCATTGA+TGG - chr5.3:20725859-20725878 MS.gene070682:intron 45.0%
! ATTTCTTCAGACGAGCGTTG+AGG + chr5.3:20724447-20724466 None:intergenic 45.0%
! CAAGTGTTGCTCCTCTGTTA+AGG + chr5.3:20721721-20721740 None:intergenic 45.0%
! CATTCAGCCTGTGATTTTGG+TGG - chr5.3:20724771-20724790 MS.gene070682:intron 45.0%
! GGATTGTTGCTAGCTGCTTT+GGG - chr5.3:20724924-20724943 MS.gene070682:intron 45.0%
! GTATAGACAGGTGATTTGGC+AGG - chr5.3:20724824-20724843 MS.gene070682:intron 45.0%
! TCCCTTTTCCAGGTTTATGG+TGG - chr5.3:20725065-20725084 MS.gene070682:intron 45.0%
! TCTGCACCTCCGATTTGATA+AGG - chr5.3:20724533-20724552 MS.gene070682:CDS 45.0%
!! AGGCCAAACATGGCATTGAT+GGG - chr5.3:20725860-20725879 MS.gene070682:intron 45.0%
!! GCTGTGATATTGGCTTCACA+AGG + chr5.3:20723175-20723194 None:intergenic 45.0%
!! GTTGATGATAGGTTGGACAC+AGG - chr5.3:20725813-20725832 MS.gene070682:intron 45.0%
!! TCATTTTGGTGTGGTGGTTG+AGG - chr5.3:20722328-20722347 MS.gene070682:intron 45.0%
!! AATAAAAAAAATAGTTATAT+TGG + chr5.3:20725551-20725570 None:intergenic 5.0%
AGCTTCCTGAAGTGTACTCG+TGG - chr5.3:20723792-20723811 MS.gene070682:intron 50.0%
AGGTCTGAACCAGCAAAGGA+TGG - chr5.3:20723412-20723431 MS.gene070682:CDS 50.0%
ATGGAGGTCTGAACCAGCAA+AGG - chr5.3:20723408-20723427 MS.gene070682:CDS 50.0%
CACGCTCAAACGGCAAAGTA+AGG + chr5.3:20725148-20725167 None:intergenic 50.0%
CAGTTGGCTGTTGCATGTAC+AGG + chr5.3:20723872-20723891 None:intergenic 50.0%
CGGATTGTTGCTAGCTGCTT+TGG - chr5.3:20724923-20724942 MS.gene070682:intron 50.0%
CTAGACATTCACGCCTTCTG+AGG - chr5.3:20723023-20723042 MS.gene070682:intron 50.0%
CTCAAACTCTGACCACTCCA+TGG + chr5.3:20725906-20725925 None:intergenic 50.0%
CTGTACATGCAACAGCCAAC+TGG - chr5.3:20723870-20723889 MS.gene070682:CDS 50.0%
GGCACATGCAATCAGGAACA+GGG + chr5.3:20726006-20726025 None:intergenic 50.0%
GGTCTGAACCAGCAAAGGAT+GGG - chr5.3:20723413-20723432 MS.gene070682:CDS 50.0%
GTCAGAGTTTGAGCATGCAG+TGG - chr5.3:20725913-20725932 MS.gene070682:intron 50.0%
GTCTGAACCAGCAAAGGATG+GGG - chr5.3:20723414-20723433 MS.gene070682:CDS 50.0%
TACAGGTGCAGCCTTGATTC+TGG + chr5.3:20723855-20723874 None:intergenic 50.0%
TCTGAACCAGCAAAGGATGG+GGG - chr5.3:20723415-20723434 MS.gene070682:CDS 50.0%
TGCAACAGCCAACTGGTATC+TGG - chr5.3:20723877-20723896 MS.gene070682:CDS 50.0%
TGGCACATGCAATCAGGAAC+AGG + chr5.3:20726007-20726026 None:intergenic 50.0%
! AAGTGTACTCGTGGAGCACT+AGG - chr5.3:20723801-20723820 MS.gene070682:intron 50.0%
! ACAGGTGATTTGGCAGGGAA+GGG - chr5.3:20724830-20724849 MS.gene070682:intron 50.0%
! AGTGTACTCGTGGAGCACTA+GGG - chr5.3:20723802-20723821 MS.gene070682:intron 50.0%
! CTCAGAAGGCGTGAATGTCT+AGG + chr5.3:20723025-20723044 None:intergenic 50.0%
! GCCCATTCAGCCTGTGATTT+TGG - chr5.3:20724768-20724787 MS.gene070682:intron 50.0%
! GTTCCCCATATCAGAGCACT+TGG - chr5.3:20724393-20724412 MS.gene070682:intron 50.0%
!! CGCAGGTGTTGGATATCCAT+AGG + chr5.3:20724342-20724361 None:intergenic 50.0%
!! TCAGCCTGTGATTTTGGTGG+AGG - chr5.3:20724774-20724793 MS.gene070682:intron 50.0%
ACGATGCCCAATGACTCCTG+AGG - chr5.3:20724896-20724915 MS.gene070682:intron 55.0%
AGCAACAATCCGACCTCCTC+AGG + chr5.3:20724915-20724934 None:intergenic 55.0%
ATGCCCAATGACTCCTGAGG+AGG - chr5.3:20724899-20724918 MS.gene070682:intron 55.0%
CCAATGACTCCTGAGGAGGT+CGG - chr5.3:20724903-20724922 MS.gene070682:intron 55.0%
CGACCTCCTCAGGAGTCATT+GGG + chr5.3:20724905-20724924 None:intergenic 55.0%
GTGCTCCACGAGTACACTTC+AGG + chr5.3:20723800-20723819 None:intergenic 55.0%
GTTTCTCTGCAGCGCATTCG+AGG - chr5.3:20723709-20723728 MS.gene070682:intron 55.0%
TACGTACCCCCATCCTTTGC+TGG + chr5.3:20723424-20723443 None:intergenic 55.0%
TGGCTGCTCCACCATAAACC+TGG + chr5.3:20725076-20725095 None:intergenic 55.0%
! AGCGTTGAGGATTGCGTAGG+CGG + chr5.3:20724434-20724453 None:intergenic 55.0%
! AGGTTTATGGTGGAGCAGCC+AGG - chr5.3:20725075-20725094 MS.gene070682:intron 55.0%
! GACAGGTGATTTGGCAGGGA+AGG - chr5.3:20724829-20724848 MS.gene070682:intron 55.0%
!! ACGAGCGTTGAGGATTGCGT+AGG + chr5.3:20724437-20724456 None:intergenic 55.0%
CCGACCTCCTCAGGAGTCAT+TGG + chr5.3:20724906-20724925 None:intergenic 60.0%
GCTGCACAGCGCTCCTCAGA+AGG + chr5.3:20723039-20723058 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 20721704 20726033 20721704 ID=MS.gene070682
chr5.3 mRNA 20721704 20726033 20721704 ID=MS.gene070682.t1;Parent=MS.gene070682
chr5.3 exon 20725938 20726033 20725938 ID=MS.gene070682.t1.exon1;Parent=MS.gene070682.t1
chr5.3 CDS 20725938 20726033 20725938 ID=cds.MS.gene070682.t1;Parent=MS.gene070682.t1
chr5.3 exon 20724671 20724733 20724671 ID=MS.gene070682.t1.exon2;Parent=MS.gene070682.t1
chr5.3 CDS 20724671 20724733 20724671 ID=cds.MS.gene070682.t1;Parent=MS.gene070682.t1
chr5.3 exon 20724474 20724555 20724474 ID=MS.gene070682.t1.exon3;Parent=MS.gene070682.t1
chr5.3 CDS 20724474 20724555 20724474 ID=cds.MS.gene070682.t1;Parent=MS.gene070682.t1
chr5.3 exon 20724301 20724371 20724301 ID=MS.gene070682.t1.exon4;Parent=MS.gene070682.t1
chr5.3 CDS 20724301 20724371 20724301 ID=cds.MS.gene070682.t1;Parent=MS.gene070682.t1
chr5.3 exon 20724117 20724205 20724117 ID=MS.gene070682.t1.exon5;Parent=MS.gene070682.t1
chr5.3 CDS 20724117 20724205 20724117 ID=cds.MS.gene070682.t1;Parent=MS.gene070682.t1
chr5.3 exon 20723813 20724016 20723813 ID=MS.gene070682.t1.exon6;Parent=MS.gene070682.t1
chr5.3 CDS 20723813 20724016 20723813 ID=cds.MS.gene070682.t1;Parent=MS.gene070682.t1
chr5.3 exon 20723183 20723453 20723183 ID=MS.gene070682.t1.exon7;Parent=MS.gene070682.t1
chr5.3 CDS 20723183 20723453 20723183 ID=cds.MS.gene070682.t1;Parent=MS.gene070682.t1
chr5.3 exon 20722751 20722978 20722751 ID=MS.gene070682.t1.exon8;Parent=MS.gene070682.t1
chr5.3 CDS 20722751 20722978 20722751 ID=cds.MS.gene070682.t1;Parent=MS.gene070682.t1
chr5.3 exon 20722463 20722660 20722463 ID=MS.gene070682.t1.exon9;Parent=MS.gene070682.t1
chr5.3 CDS 20722463 20722660 20722463 ID=cds.MS.gene070682.t1;Parent=MS.gene070682.t1
chr5.3 exon 20721704 20721913 20721704 ID=MS.gene070682.t1.exon10;Parent=MS.gene070682.t1
chr5.3 CDS 20721704 20721913 20721704 ID=cds.MS.gene070682.t1;Parent=MS.gene070682.t1
Gene Sequence

>MS.gene070682

ATGGAGCTTAGCTTCCTTAACAGAGGAGCAACACTTGTTGTTTCTCTTTCCTTGTTCCTTTTCATTCATCATGTCTCTTCAACTTCTATGTACTCGGTGAGTTTAATAAATCTTCAATTTTTTTTCTTTCTCGGAAGTTTTATTTTTAATTCTTTTTCTAATTTGGGTGGAACAGTAACATTACCGTTATGTCTTTTATTTCATTTTCAGTAATGTTTTTTTCCTTGGATTATTTTATATGTGTGTTTGTTTAGTTAATTTCTGATTTGTTTTTTTTTTTTGTTAGTTGGTTTTAGTTTATTATCAGAAATCTTTTGAGTTTCATAGTAAATTTTATTTTAGAGAAATTTAGAAACTGGGGTTTGGTTGATCAATATTTGTGTGTGTTTTTGGGGTGAAAGAAATTATATGGGGTTTAGTTAATAAGCGTTTAGATTATTTGTTTGATGCTGTTTAGCGTTAAGATTAATTTTTGGATTGGGAATTTGACAGCTTAGAAAATGAAAGTAGCGAGTGAGAGAAAGGTATAATGGAATTAATTGGGTTGACTGAGTTAGCTAAATTTGTGTAACTAATTTAACAGCCTATTAATTATAGATCACATGGTTTAGGACATGCTATTACTCATTTTGGTGTGGTGGTTGAGGTTCTTTGTTGGATAGTTACTTGCAATTAATTTGAAGAAAAGGGAATTAACAAAAAAAGGGAATTAGGAAAATAAAATAAAATTGAAGGGGCAGAATTAGGAATTGTTTAGGTAAATATTATAAAATTGTGTCCAATGGAAGGCTTAATATTGATAATGAAAAAAAAAAAAATTGTCAAAAAAAAATGATATGATGCTTGGTTAACTTGCCTTGTAAAAGTCACATAATTCCTGTTTAGATATAAACAACTTAATTAAACGCTTATGGCATAAGCACTTATCATATAGTGCTTATATGTAAGCTATTTCTGTAGCAAATGATAAATTAAAGTCAAATTGTTCTTGTATAAGCTATAACCTGTTTTCATAAGCTATCTTGAAATTTATATGATTGGAAATAAGCTGAAAACAACTTATGCACATGTCATAAGCTATTTCTATAAGCTCTCCCAAACAGTCTCATAAGTACTTATGGTAGTTGATAAACTCAAATAGACCAATCCAAACATGCTCAATATCTATTAGAATGAATAACATCATCGTGTTCATGCTGTTTAATTCCTAGTTTTTCCTTTCTTTCGTGCCTTAGCATCTCGTTATGATTTTAGTTTATTGGCTTTCCATACCTTATAACTTGGAACTTGCTTTATGTAGGAAATGAATCCTATAAAACCTAGACATTCACGCCTTCTGAGGAGCGCTGTGCAGCGTGAAACAGTAAGTTAATTTAGCAGTGAATGCTTTCTTTAAACAACTTTTATTTTGGTTTTTGTTAGTTTTGATTGTTCATTTTATATATTTTATAATTTGTTATTGTTTTCTCCTTGTGAAGCCAATATCACAGCTTTCCGAAATATGGACACCACTAGAAAATCAAGGTTGGAAATCTTGCGATGAATCGGAAAATAGGCCAAGTGAGTGAAAGTTACTTTATTTTATCTTTGAGAACACATGATGTTTGGTTATGTATATTTCTTTATCTAATCATTTTGTCACTGGAACTTGAATTGAAGCAGCATTACCAGAGAAGTCTGAAGGATACATCCAGGTATTTCTTGATGGAGGTCTGAACCAGCAAAGGATGGGGGTACGTATTTTAAAGCCTTTTAATTAGATCAAGCTTTTTCTTTCGTGTATGCGAAAGATTTATAATTTTTACACGGTGTTTAATTATTTTTTTAGATATGTGATGCAGTTGCTGTTGCGAAGATACTGAATGCAACTCTTGTGATCCCATACCTTGAATTAAATCCTGTGTGGAAGGATTCAAGGTAGTGTCAGAAACTAGAACTACAATTTATTGTATTATTTTGTTCCGTGGATTGTGCACGATGTAATGTATTGATCGAGGTTGCTTATGTTTCTCTGCAGCGCATTCGAGGATATATTTGATGTGGATCATTTCATTGATGTATTAAAGGATGATGTTTCTATAGTTAAAGAGCTTCCTGAAGTGTACTCGTGGAGCACTAGGGAGTACTATTCATTGGCTATTCGAGATACCAGAATCAAGGCTGCACCTGTACATGCAACAGCCAACTGGTATCTGGAAAATGTTTTGCCTGTCCTACAAAGGTTAGTAGTTACCTGAATCTGTATTCTGTACTTTTAAATGCTAGTGCATTCATGACTTGAAATGTTTCGGACCAATAACTTTAACATTAGTCGATTCAGTGACTAAGATTATAGACACTTTTATTCATAATTGCAATTGCAACTGCTTTAGCATTTATATAACGTTATCAATTACAGAACCTGCGCTGAACATTGTTTTGAAGATTATTTATTACTTCTTTGTTTTTAAATAATCTTTCTATCGATTTTAAATAAAGATATGGCTGATTTTAAGGTGTATATTATGGGGATGCTGCAAGCTCTGTATATTCTCTTTGAATTTTGAAATTCTAATATCATGGTGTTTTGGTATGATGCAGTTATGGTGTTGCTGCAATCTCTCCATTTTCTCACCGCCTGAGTTTTGATAACTTGCCTATGGATATCCAACACCTGCGTTGTAAAGTCAACTTTCAAGCTTTAGACTTTGTTCCCCATATCAGAGCACTTGGAGATTCTCTCATCAGCCGCCTACGCAATCCTCAACGCTCGTCTGAAGAAATGAACTCCAACTACCTTCAAGAGGTCACAAATGTAGATGATAATAAAAATGCAGGGAAATTTGTTGTTCTGCACCTCCGATTTGATAAGGTATTTATCTTTTTCTCATTTATCATCATCATCATCTGTACATACAATGTTGTAAACTTTGTCTCTGTTTGTTTCTTAATTTTTCTTTTTTGTTGGTATAACTATCTTGACGTTACAGGAGTGCTAGCAACACACTCCCTGCCCTATTTTTACATGCATTTGTCTGTAAATTGAGAAAGTTTGTGTGATAATATTTTATTACAGGATATGGCCGCCCATTCAGCCTGTGATTTTGGTGGAGGAAAAGCTGAAAAATTGGCTCTTGCAAAGTATAGACAGGTGATTTGGCAGGGAAGGGTTCTTAATTCCCAATTCACTGATGAAGAGTTGAGAAGTCAGGGACGATGCCCAATGACTCCTGAGGAGGTCGGATTGTTGCTAGCTGCTTTGGGATTCGACAACAGCACTCGTTTATATCTTGCCTCTCATAAGGTTTACCCATGTTATTTTTTAGTTTGAAGCAGTAGAGTCTCATTCATTTTAATTATTAGTGCCTTATCTAAAATATCTTCCCTTTTCCAGGTTTATGGTGGAGCAGCCAGGATTTCTACTTTGAAGCAATTGTTTCCTCTGATGGAAGACAAAAAGAGCCTTACTTTGCCGTTTGAGCGTGCTCTGATAAAGGGAAAAGCTTCTCTATTAGCCGCACTTGACTACTATATTAGTATACATAGTGACATCTTCATTTCTGCTTCCCCTGGAAATATGCACAATGCATTGGTGAGTTCTCCCCTAGATTTACCATATATATGACTACATTTCATATGCATTGCCAATATTAGTAACTAGTCTAGAATACTGATAATGGACCTTGACTTAACAATGTTGAGGCCATGTTCATATTCAATTATTTATTATGAGATGCATTTGGCCATACAAACAGAATTCCTGTGAACATGGGACATTAATGAGTTTGTCATATAACAATTAATTACTCCCTCCGTATCATATTTAGAGTATTGTTTGTCTCACATTATTTGTCATTTTAAAATACCAATATAACTATTTTTTTTATTTTTTTACTGTTATACTCTTATTTAGTAAATGAAACACATTATCATAGAAATTACTAGTACACAGAATAATTTTCTTAAAAAGTGTGTATTACTATAAATGACAATGACACTTAATACATGAGATGGAGGGAGTAACACTATAAGAAATCTGTGTTTAGAACTGTCTAGACAACATATTTATGATGGTACTGCAAGGCTGTTGAAACAGTTGAATTGAATTTATAAATGATAATGAATGCTTTGTTGATGATAGGTTGGACACAGGACATACTTGAACTTGAAGACTATAAGGCCAAACATGGCATTGATGGGTCAACTGTTCTTGAACAAAACCATGGAGTGGTCAGAGTTTGAGCATGCAGTGGTTGAAGGTCATGAAAGTAGACAAGGTCAACTCAGGCTTAGAAAGCCTAAACAATCCATATACACATACCCTGTTCCTGATTGCATGTGCCAATCTTAA
Protein sequence

>MS.gene070682.t1

MELSFLNRGATLVVSLSLFLFIHHVSSTSMYSEMNPIKPRHSRLLRSAVQRETPISQLSEIWTPLENQGWKSCDESENRPTLPEKSEGYIQVFLDGGLNQQRMGICDAVAVAKILNATLVIPYLELNPVWKDSSAFEDIFDVDHFIDVLKDDVSIVKELPEVYSWSTREYYSLAIRDTRIKAAPVHATANWYLENVLPVLQSYGVAAISPFSHRLSFDNLPMDIQHLRCKVNFQALDFVPHIRALGDSLISRLRNPQRSSEEMNSNYLQEVTNVDDNKNAGKFVVLHLRFDKDMAAHSACDFGGGKAEKLALAKYRQVIWQGRVLNSQFTDEELRSQGRCPMTPEEVGLLLAALGFDNSTRLYLASHKVYGGAARISTLKQLFPLMEDKKSLTLPFERALIKGKASLLAALDYYISIHSDIFISASPGNMHNALVGHRTYLNLKTIRPNMALMGQLFLNKTMEWSEFEHAVVEGHESRQGQLRLRKPKQSIYTYPVPDCMCQS