AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069107

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069107.t1 MTR_5g032100 97.619 504 11 1 1 504 1 503 0.0 1025
MS.gene069107.t1 MTR_3g100310 68.164 512 154 1 2 504 6 517 0.0 734
MS.gene069107.t1 MTR_3g086570 39.278 443 237 8 71 504 130 549 9.61e-107 329
MS.gene069107.t1 MTR_3g005340 39.606 457 234 10 59 503 119 545 1.64e-101 316
MS.gene069107.t1 MTR_1g031590 38.261 460 243 13 56 504 119 548 3.92e-100 312
MS.gene069107.t1 MTR_4g084570 38.326 454 245 12 59 504 123 549 2.03e-98 308
MS.gene069107.t1 MTR_2g016470 39.293 481 262 13 45 503 143 615 7.10e-98 308
MS.gene069107.t1 MTR_5g059260 40.984 427 213 12 89 502 159 559 3.20e-93 295
MS.gene069107.t1 MTR_3g099480 31.648 534 288 11 9 503 43 538 1.11e-89 285
MS.gene069107.t1 MTR_4g082310 37.587 431 220 9 87 504 97 491 2.11e-89 283
MS.gene069107.t1 MTR_2g059350 34.115 469 249 9 56 499 71 504 2.21e-85 273
MS.gene069107.t1 MTR_5g042200 36.538 468 243 12 54 503 47 478 2.27e-85 272
MS.gene069107.t1 MTR_3g096020 33.849 517 271 11 11 502 116 586 1.29e-84 273
MS.gene069107.t1 MTR_5g064620 33.817 482 234 9 57 503 81 512 2.37e-84 270
MS.gene069107.t1 MTR_3g086570 43.168 322 153 7 71 385 130 428 2.52e-84 268
MS.gene069107.t1 MTR_4g110030 34.728 478 243 12 56 499 63 505 3.14e-83 267
MS.gene069107.t1 MTR_6g016015 35.198 429 230 9 86 501 114 507 1.51e-82 265
MS.gene069107.t1 MTR_5g099010 36.947 452 235 13 61 503 90 500 5.03e-82 266
MS.gene069107.t1 MTR_4g125460 35.153 458 244 11 60 503 61 479 2.30e-81 261
MS.gene069107.t1 MTR_4g132590 37.029 451 259 11 71 503 50 493 4.03e-81 261
MS.gene069107.t1 MTR_4g100810 36.444 450 241 14 61 503 79 490 5.04e-81 262
MS.gene069107.t1 MTR_3g115220 34.821 448 251 9 61 503 82 493 8.10e-81 262
MS.gene069107.t1 MTR_4g132590 37.029 451 259 11 71 503 149 592 5.27e-80 261
MS.gene069107.t1 MTR_4g125460 35.153 458 244 11 60 503 200 618 7.93e-80 261
MS.gene069107.t1 MTR_1g102030 34.979 466 243 14 58 503 58 483 1.09e-78 255
MS.gene069107.t1 MTR_5g012770 34.714 507 258 15 9 503 50 495 1.15e-74 244
MS.gene069107.t1 MTR_4g019210 32.967 455 254 10 61 502 205 621 3.24e-74 247
MS.gene069107.t1 MTR_3g026650 34.947 475 250 10 43 502 126 556 2.32e-73 243
MS.gene069107.t1 MTR_4g023030 34.505 455 247 10 60 502 201 616 4.22e-71 238
MS.gene069107.t1 MTR_5g081690 33.042 457 253 11 61 503 94 511 7.90e-70 232
MS.gene069107.t1 MTR_3g026650 34.321 405 210 8 43 435 126 486 2.34e-57 198
MS.gene069107.t1 MTR_1g101990 34.304 309 167 8 201 503 1 279 4.01e-48 169
MS.gene069107.t1 MTR_4g010990 27.527 465 264 13 53 503 34 439 1.52e-43 160
MS.gene069107.t1 MTR_3g026650 32.390 318 159 8 43 348 126 399 1.96e-37 142
MS.gene069107.t1 MTR_4g010990 26.471 408 234 11 103 503 2 350 2.70e-35 135
MS.gene069107.t1 MTR_7g017330 28.358 335 200 6 81 405 92 396 4.06e-30 124
MS.gene069107.t1 MTR_3g092590 28.783 337 188 8 73 393 76 376 2.18e-29 122
MS.gene069107.t1 MTR_3g092590 27.475 404 236 10 10 393 10 376 5.67e-29 121
MS.gene069107.t1 MTR_1g101985 46.226 106 56 1 58 162 58 163 1.64e-26 106
MS.gene069107.t1 MTR_7g064210 36.471 170 77 3 223 385 37 182 2.79e-24 105
MS.gene069107.t1 MTR_1g007390 26.341 410 239 11 10 393 10 382 3.55e-23 103
MS.gene069107.t1 MTR_4g008200 24.444 360 202 12 85 439 33 327 1.25e-20 94.0
MS.gene069107.t1 MTR_4g008200 24.444 360 202 12 85 439 84 378 3.12e-20 93.2
MS.gene069107.t1 MTR_2g038320 24.377 361 199 11 85 439 90 382 1.41e-19 91.3
MS.gene069107.t1 MTR_3g077710 53.125 64 11 1 292 355 4 48 1.29e-13 66.6
MS.gene069107.t1 MTR_8g017030 49.206 63 12 1 293 355 5 47 8.86e-12 61.2
MS.gene069107.t1 MTR_1g095680 39.130 92 44 2 228 319 16 95 3.39e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069107.t1 AT5G65470 73.428 493 123 3 17 503 13 503 0.0 762
MS.gene069107.t1 AT4G24530 70.650 477 140 0 27 503 41 517 0.0 726
MS.gene069107.t1 AT3G26370 38.612 461 246 9 58 504 120 557 1.56e-108 334
MS.gene069107.t1 AT1G35510 40.132 456 232 10 59 503 132 557 2.81e-103 321
MS.gene069107.t1 AT1G29200 41.150 452 240 11 71 503 50 494 2.31e-96 300
MS.gene069107.t1 AT1G29200 41.150 452 240 11 71 503 50 494 2.31e-96 300
MS.gene069107.t1 AT1G38065 39.468 451 228 12 68 502 17 438 8.53e-96 298
MS.gene069107.t1 AT1G38065 40.278 432 213 12 87 502 26 428 5.11e-95 296
MS.gene069107.t1 AT1G29200 41.150 452 240 11 71 503 166 610 7.00e-95 300
MS.gene069107.t1 AT1G38131 40.278 432 213 12 87 502 155 557 2.14e-93 296
MS.gene069107.t1 AT1G29200 41.844 423 224 10 96 503 5 420 7.59e-93 289
MS.gene069107.t1 AT2G01480 37.762 429 232 11 83 503 151 552 5.33e-91 289
MS.gene069107.t1 AT1G14970 36.784 454 252 12 58 503 7 433 3.99e-90 283
MS.gene069107.t1 AT1G14970 37.500 424 233 9 87 503 9 407 1.69e-89 280
MS.gene069107.t1 AT1G14970 36.403 467 261 13 46 503 114 553 1.24e-88 283
MS.gene069107.t1 AT1G14020 38.657 432 214 11 87 504 91 485 1.28e-88 281
MS.gene069107.t1 AT4G38390 39.423 416 215 9 90 503 126 506 3.63e-87 278
MS.gene069107.t1 AT1G62330 36.853 464 263 15 61 502 194 649 1.68e-85 277
MS.gene069107.t1 AT3G02250 34.534 472 245 12 59 501 62 498 1.36e-84 270
MS.gene069107.t1 AT1G22460 33.262 469 253 8 56 503 129 558 1.97e-84 271
MS.gene069107.t1 AT1G04910 35.099 453 252 10 58 503 58 475 2.74e-84 270
MS.gene069107.t1 AT2G03280 37.995 429 216 11 87 501 88 480 7.07e-84 268
MS.gene069107.t1 AT1G20550 37.045 440 230 12 71 503 91 490 8.20e-84 270
MS.gene069107.t1 AT5G64600 35.637 463 240 9 60 502 90 514 8.79e-84 269
MS.gene069107.t1 AT2G37980 35.371 458 245 10 59 503 197 616 1.83e-83 271
MS.gene069107.t1 AT1G11990 36.345 476 266 14 58 504 145 612 2.07e-82 268
MS.gene069107.t1 AT5G15740 33.898 472 248 13 59 501 62 498 3.29e-82 264
MS.gene069107.t1 AT1G76270 37.204 422 222 10 86 503 105 487 3.72e-81 263
MS.gene069107.t1 AT1G11990 36.009 461 251 13 58 504 160 590 3.74e-81 264
MS.gene069107.t1 AT4G16650 38.528 462 233 15 50 503 86 504 8.14e-81 262
MS.gene069107.t1 AT4G16650 38.528 462 233 15 50 503 86 504 8.14e-81 262
MS.gene069107.t1 AT2G01480 37.949 390 210 9 83 466 151 514 3.58e-80 259
MS.gene069107.t1 AT2G03280 35.746 456 216 12 87 501 88 507 8.60e-78 253
MS.gene069107.t1 AT5G35570 31.855 496 286 10 22 503 184 641 1.05e-75 251
MS.gene069107.t1 AT3G54100 31.931 523 289 11 9 502 127 611 1.91e-75 250
MS.gene069107.t1 AT2G44500 34.812 451 252 6 60 502 145 561 3.39e-74 245
MS.gene069107.t1 AT5G01100 33.550 462 252 11 57 502 186 608 7.30e-74 246
MS.gene069107.t1 AT3G07900 32.751 458 250 9 61 502 157 572 7.48e-70 234
MS.gene069107.t1 AT3G26370 43.116 276 125 7 58 324 120 372 1.52e-67 223
MS.gene069107.t1 AT5G63390 31.788 453 263 9 61 503 133 549 4.56e-64 218
MS.gene069107.t1 AT1G52630 27.655 452 259 14 60 503 45 436 1.52e-46 168
MS.gene069107.t1 AT2G44500 33.788 293 155 4 61 348 146 404 2.35e-41 153
MS.gene069107.t1 AT1G52630 26.355 406 235 12 103 503 2 348 1.95e-37 141
MS.gene069107.t1 AT3G03810 31.385 325 179 9 81 393 83 375 2.30e-34 137
MS.gene069107.t1 AT3G03810 31.385 325 179 9 81 393 83 375 2.30e-34 137
MS.gene069107.t1 AT3G30300 28.529 333 200 7 84 407 97 400 1.95e-29 122
MS.gene069107.t1 AT3G30300 28.529 333 200 7 84 407 97 400 1.95e-29 122
MS.gene069107.t1 AT3G21190 25.485 361 200 9 87 443 90 385 2.84e-23 102
MS.gene069107.t1 AT1G51630 25.613 367 192 10 87 443 91 386 5.57e-23 101

Find 129 sgRNAs with CRISPR-Local

Find 275 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTTCATTGATGTATTAA+AGG 0.217296 5.4:-22247108 MS.gene069107:CDS
CGGATTGTTGCTAGCTGCTT+TGG 0.252846 5.4:-22245921 MS.gene069107:CDS
TCAACTCTTCATCAGTGAAT+TGG 0.272382 5.4:+22245981 None:intergenic
TATCACAGCTTTCGGAAATA+TGG 0.274971 5.4:-22247670 MS.gene069107:CDS
AATCTCCAAGTGCTCTGATA+TGG 0.282135 5.4:+22246448 None:intergenic
CTTGCGATGAATCGGAAAAT+AGG 0.283776 5.4:-22247619 MS.gene069107:CDS
CTCTTCAACTTCTATGTATT+CGG 0.284297 5.4:-22249110 MS.gene069107:intron
GCCCATTCAGCCTGTGATTT+CGG 0.293347 5.4:-22246076 MS.gene069107:CDS
ATTTCCGAAAGCTGTGATAT+TGG 0.307364 5.4:+22247674 None:intergenic
AAACTCAGGCGGTGAGAAAA+TGG 0.310777 5.4:+22246540 None:intergenic
CAAAGTATAGACAGGTGATT+TGG 0.333507 5.4:-22246024 MS.gene069107:CDS
TGCAACAGCCAACTGGTATC+TGG 0.335170 5.4:-22246979 MS.gene069107:CDS
TACTGGTGCAGCCTTGATTC+TGG 0.343702 5.4:+22247004 None:intergenic
CCGACTTCCTCGGGAGTCAT+TGG 0.362184 5.4:+22245941 None:intergenic
AGGAACAGGGTATGTGTATA+TGG 0.362734 5.4:+22244837 None:intergenic
CATTTCTTCAGACGAGCGTT+GGG 0.364333 5.4:+22246401 None:intergenic
GGATTGTTGCTAGCTGCTTT+GGG 0.372795 5.4:-22245920 MS.gene069107:CDS
TGACCCATCAATGCCATGTT+TGG 0.373511 5.4:+22244964 None:intergenic
CAACTCTTCATCAGTGAATT+GGG 0.375810 5.4:+22245982 None:intergenic
AGGTAACTACTAACCTTTGT+AGG 0.380843 5.4:+22246940 None:intergenic
ACTTGAACTTGAAGACTATA+AGG 0.381120 5.4:-22244987 MS.gene069107:CDS
GAAGCCAATATCACAGCTTT+CGG 0.401834 5.4:-22247678 MS.gene069107:intron
AAGGCCAAACATGGCATTGA+TGG 0.402671 5.4:-22244968 MS.gene069107:CDS
TGGCACATGCAATCAGGAAC+AGG 0.405776 5.4:+22244823 None:intergenic
TTTGAGCGTGCTCTGATAAA+GGG 0.411174 5.4:-22245667 MS.gene069107:CDS
CTACATTTGTGACCTCTTGA+AGG 0.413082 5.4:+22246367 None:intergenic
TTTATATCTTGCCTCTCATA+AGG 0.413884 5.4:-22245879 MS.gene069107:intron
CACCGAAATCACAGGCTGAA+TGG 0.415308 5.4:+22246074 None:intergenic
TACATCCAGGTATTTCTTGA+TGG 0.422805 5.4:-22247467 MS.gene069107:CDS
GTGCTCCACGAGTACACTTC+AGG 0.423676 5.4:+22247059 None:intergenic
TGTGACCTCTTGAAGGTAGT+TGG 0.429082 5.4:+22246374 None:intergenic
ATCTCCAAGTGCTCTGATAT+GGG 0.429348 5.4:+22246449 None:intergenic
ACAGGATTTAATTCAAGGTA+TGG 0.429350 5.4:+22247273 None:intergenic
TCCACACAGGATTTAATTCA+AGG 0.432589 5.4:+22247268 None:intergenic
TCTGCACCTTCGATTTGATA+AGG 0.434704 5.4:-22246313 MS.gene069107:intron
GTTTCTCTGCAGCGCATTCG+AGG 0.443894 5.4:-22247147 MS.gene069107:intron
CAGTTGGCTGTTGCATGTAC+TGG 0.452590 5.4:+22246987 None:intergenic
AATGCATTGTGCATATTTCC+TGG 0.457863 5.4:+22245574 None:intergenic
TGATGAAGAGTTGAGAAGTC+AGG 0.457882 5.4:-22245972 MS.gene069107:CDS
TTGGAAATCTTGCGATGAAT+CGG 0.459724 5.4:-22247627 MS.gene069107:CDS
TATGTGTATATGGATTGTTT+AGG 0.459764 5.4:+22244847 None:intergenic
ACCGAAATCACAGGCTGAAT+GGG 0.460554 5.4:+22246075 None:intergenic
AGCAACAATCCGACTTCCTC+GGG 0.460817 5.4:+22245932 None:intergenic
ATCCTGTGTGGAAGGATTCA+AGG 0.461236 5.4:-22247257 MS.gene069107:intron
GACAGGTGATTTGGCAGGGA+AGG 0.464416 5.4:-22246015 MS.gene069107:CDS
TTTGAGCATGCAGTGGTTGA+AGG 0.466848 5.4:-22244907 MS.gene069107:CDS
CGACTTCCTCGGGAGTCATT+GGG 0.469542 5.4:+22245942 None:intergenic
ATTCAGCCTGTGATTTCGGT+GGG 0.473135 5.4:-22246072 MS.gene069107:CDS
AAGTGTACTCGTGGAGCACT+AGG 0.473525 5.4:-22247055 MS.gene069107:CDS
GATTGCGAAGGCGGCTGATG+AGG 0.474687 5.4:+22246424 None:intergenic
ATGTATCCTTCAGACTTCTC+TGG 0.474788 5.4:+22247485 None:intergenic
GAAGCAATTGTTTCCTCTGA+TGG 0.475940 5.4:-22245716 MS.gene069107:CDS
CAAGTGTTGCTCCTCTGTTA+AGG 0.479005 5.4:+22249173 None:intergenic
TAACCTTGGCACATGCAATC+AGG 0.479867 5.4:+22244817 None:intergenic
ATCTTCATTTCTGCTTCACC+AGG 0.483138 5.4:-22245592 MS.gene069107:CDS
GGTCTGAACCAGCAAAGGAT+GGG 0.485650 5.4:-22247443 MS.gene069107:CDS
TTTATCAGAGCACGCTCAAA+AGG 0.485770 5.4:+22245670 None:intergenic
AAGAGACATGATGAATGAAA+AGG 0.486074 5.4:+22249128 None:intergenic
CACTAGGGAGTACTATTCAT+TGG 0.486981 5.4:-22247039 MS.gene069107:CDS
CATTCAGCCTGTGATTTCGG+TGG 0.489218 5.4:-22246073 MS.gene069107:CDS
GCTGCACGGCGCTCCTCAGA+AGG 0.491312 5.4:+22247821 None:intergenic
TTGCTTCAAAGTAGAAATCC+TGG 0.492690 5.4:+22245732 None:intergenic
TCAGCCTGTGATTTCGGTGG+GGG 0.500201 5.4:-22246070 MS.gene069107:CDS
ATTGCGAAGGCGGCTGATGA+GGG 0.502270 5.4:+22246425 None:intergenic
AGGAAATATGCACAATGCAT+TGG 0.503143 5.4:-22245572 MS.gene069107:intron
CACGCTCAAAAGGCAAAGTA+AGG 0.503931 5.4:+22245680 None:intergenic
GTATAGACAGGTGATTTGGC+AGG 0.507391 5.4:-22246020 MS.gene069107:CDS
GTTCCCCATATCAGAGCACT+TGG 0.511029 5.4:-22246453 MS.gene069107:CDS
AGGTCTATGGTGGAGCAGCC+AGG 0.513876 5.4:-22245750 MS.gene069107:CDS
ACTTACTGTTTCACGCTGCA+CGG 0.513950 5.4:+22247807 None:intergenic
GTCTGAACCAGCAAAGGATG+GGG 0.514933 5.4:-22247442 MS.gene069107:CDS
CAGTACATGCAACAGCCAAC+TGG 0.517080 5.4:-22246986 MS.gene069107:CDS
GGCTCTTGCAAAGTATAGAC+AGG 0.518636 5.4:-22246032 MS.gene069107:CDS
TAGCAACAATCCGACTTCCT+CGG 0.519476 5.4:+22245931 None:intergenic
AGGTCTGAACCAGCAAAGGA+TGG 0.519610 5.4:-22247444 MS.gene069107:CDS
TGACTTTACAACGCAGGTGT+TGG 0.521199 5.4:+22246496 None:intergenic
TCATTTCTTCAGACGAGCGT+TGG 0.523115 5.4:+22246400 None:intergenic
CTCAGAAGGCGTGAGTGTCT+AGG 0.527498 5.4:+22247835 None:intergenic
AGCGTTGGGGATTGCGAAGG+CGG 0.528379 5.4:+22246415 None:intergenic
ACTACTAACCTTTGTAGGAC+AGG 0.531765 5.4:+22246945 None:intergenic
ACAGGTGATTTGGCAGGGAA+GGG 0.537749 5.4:-22246014 MS.gene069107:CDS
TGAATTAAATCCTGTGTGGA+AGG 0.541914 5.4:-22247265 MS.gene069107:CDS
CAGGATTTAATTCAAGGTAT+GGG 0.544888 5.4:+22247274 None:intergenic
CACCACTAGAAAATCAAGGT+TGG 0.561009 5.4:-22247646 MS.gene069107:CDS
CGCAGGTGTTGGATATCCAT+AGG 0.561306 5.4:+22246507 None:intergenic
CTCAAACTCTGACCACTCCA+TGG 0.563819 5.4:+22244924 None:intergenic
TGTAAGCAATCTATTAACCT+TGG 0.565987 5.4:+22244803 None:intergenic
AGGCCAAACATGGCATTGAT+GGG 0.568191 5.4:-22244967 MS.gene069107:CDS
TATTCGAGATACCAGAATCA+AGG 0.571849 5.4:-22247015 MS.gene069107:CDS
GAAGTCTGAAGGATACATCC+AGG 0.574141 5.4:-22247480 MS.gene069107:CDS
TACCTTGAATCCTTCCACAC+AGG 0.577185 5.4:+22247255 None:intergenic
ATGGAGGTCTGAACCAGCAA+AGG 0.578859 5.4:-22247448 MS.gene069107:CDS
GTTCCTGATTGCATGTGCCA+AGG 0.580025 5.4:-22244820 MS.gene069107:CDS
TAAATACCTTATCAAATCGA+AGG 0.581327 5.4:+22246307 None:intergenic
TTCAGCCTGTGATTTCGGTG+GGG 0.582553 5.4:-22246071 MS.gene069107:CDS
TGGACACCACTAGAAAATCA+AGG 0.585064 5.4:-22247650 MS.gene069107:CDS
AAAGTAGACAAGGTCAACTC+AGG 0.585115 5.4:-22244879 MS.gene069107:CDS
ATCCAGGTATTTCTTGATGG+AGG 0.585774 5.4:-22247464 MS.gene069107:CDS
CTAGACACTCACGCCTTCTG+AGG 0.586628 5.4:-22247834 MS.gene069107:CDS
AGCTTCCTGAAGTGTACTCG+TGG 0.588164 5.4:-22247064 MS.gene069107:CDS
GTTAAGGAAGCTAAGCTCCA+TGG 0.590912 5.4:+22249189 None:intergenic
ATTCGAGGATATATTTGATG+TGG 0.596280 5.4:-22247132 MS.gene069107:CDS
GAACTCCAACTACCTTCAAG+AGG 0.598101 5.4:-22246379 MS.gene069107:CDS
GCATTACCAGAGAAGTCTGA+AGG 0.598413 5.4:-22247491 MS.gene069107:CDS
GATGAAGAGTTGAGAAGTCA+GGG 0.598917 5.4:-22245971 MS.gene069107:CDS
GACCTCCATCAAGAAATACC+TGG 0.602514 5.4:+22247462 None:intergenic
TAGGCAAGTTATCAAAACTC+AGG 0.602743 5.4:+22246526 None:intergenic
TATAGACAGGTGATTTGGCA+GGG 0.613330 5.4:-22246019 MS.gene069107:CDS
AGTGTACTCGTGGAGCACTA+GGG 0.613717 5.4:-22247054 MS.gene069107:CDS
TCTCCAAGTGCTCTGATATG+GGG 0.629289 5.4:+22246450 None:intergenic
ACGAGCGTTGGGGATTGCGA+AGG 0.631745 5.4:+22246412 None:intergenic
ATTTCTTCAGACGAGCGTTG+GGG 0.633988 5.4:+22246402 None:intergenic
GAGCTTAGCTTCCTTAACAG+AGG 0.634652 5.4:-22249184 MS.gene069107:CDS
GAAGACTATAAGGCCAAACA+TGG 0.637671 5.4:-22244977 MS.gene069107:CDS
ACTGTTCTTGAACAAAACCA+TGG 0.641651 5.4:-22244941 MS.gene069107:CDS
CCAATGACTCCCGAGGAAGT+CGG 0.643324 5.4:-22245941 MS.gene069107:CDS
GTTGATGATAGGTTGGACAC+AGG 0.651170 5.4:-22245014 MS.gene069107:intron
GGCACATGCAATCAGGAACA+GGG 0.652188 5.4:+22244824 None:intergenic
ACATGATTAAACCTTATGAG+AGG 0.655571 5.4:+22245868 None:intergenic
GTCAGAGTTTGAGCATGCAG+TGG 0.658254 5.4:-22244914 MS.gene069107:CDS
ATGATGAATGAAAAGGAACA+AGG 0.675260 5.4:+22249135 None:intergenic
GAAAGTTGACTTTACAACGC+AGG 0.676645 5.4:+22246490 None:intergenic
ACCTTGAATTAAATCCTGTG+TGG 0.679224 5.4:-22247269 MS.gene069107:CDS
GAAGGTCATGAAAGTAGACA+AGG 0.680741 5.4:-22244889 MS.gene069107:CDS
TCTGAACCAGCAAAGGATGG+GGG 0.685694 5.4:-22247441 MS.gene069107:intron
GCAAGTTATCAAAACTCAGG+CGG 0.692975 5.4:+22246529 None:intergenic
TCTTGAACAAAACCATGGAG+TGG 0.694603 5.4:-22244936 MS.gene069107:CDS
ACTAATGTAGTAGTCAAGTG+CGG 0.713873 5.4:+22245627 None:intergenic
ACGTTGCCCAATGACTCCCG+AGG 0.739166 5.4:-22245948 MS.gene069107:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAATAAAATTGAAG+GGG - chr5.4:22245566-22245585 MS.gene069107:intron 10.0%
!! AACAAAATAATACAATAAAT+TGG + chr5.4:22246792-22246811 None:intergenic 10.0%
!! GAAAATAAAATAAAATTGAA+GGG - chr5.4:22245565-22245584 MS.gene069107:intron 10.0%
!!! AAAGATTTATAATTTTTACA+CGG - chr5.4:22246637-22246656 MS.gene069107:intron 10.0%
!! AAATGAAATAAAAGACATAA+CGG + chr5.4:22245002-22245021 None:intergenic 15.0%
!! GGAAAATAAAATAAAATTGA+AGG - chr5.4:22245564-22245583 MS.gene069107:intron 15.0%
!! TATCTTGAAATTTATATGAT+TGG - chr5.4:22245868-22245887 MS.gene069107:intron 15.0%
!!! AATCTTCATTTTTTTTTTCT+CGG - chr5.4:22244927-22244946 MS.gene069107:CDS 15.0%
!!! ATCGATTTTAAATAAAGATA+TGG - chr5.4:22247322-22247341 MS.gene069107:CDS 15.0%
!!! ATTTTGAAATTCTAATATCA+TGG - chr5.4:22247400-22247419 MS.gene069107:intron 15.0%
!!! CATATATATGGTAAATTTTA+GGG + chr5.4:22248465-22248484 None:intergenic 15.0%
!!! GTTTGTGTAATAATATTTTA+TGG - chr5.4:22247892-22247911 MS.gene069107:intron 15.0%
!!! TCATATATATGGTAAATTTT+AGG + chr5.4:22248466-22248485 None:intergenic 15.0%
!!! TTAAGATTAATTTTTGGATT+GGG - chr5.4:22245311-22245330 MS.gene069107:intron 15.0%
!! AAAAAAAATGATATGATGCT+TGG - chr5.4:22245674-22245693 MS.gene069107:CDS 20.0%
!! AAAAGATTGATCCAATTAAA+AGG + chr5.4:22246602-22246621 None:intergenic 20.0%
!! AAAAGGGAATTAACAAAAAA+AGG - chr5.4:22245535-22245554 MS.gene069107:intron 20.0%
!! AAAGGGAATTAACAAAAAAA+GGG - chr5.4:22245536-22245555 MS.gene069107:intron 20.0%
!! AAGGTATAAAGGAATTAATT+GGG - chr5.4:22245374-22245393 MS.gene069107:intron 20.0%
!! ACAATACTCTAAATATGATA+CGG + chr5.4:22248675-22248694 None:intergenic 20.0%
!! ACCAAAAAAAAAAACAAATC+AGG + chr5.4:22245112-22245131 None:intergenic 20.0%
!! ATTAACAAAAAAAGGGAATT+AGG - chr5.4:22245543-22245562 MS.gene069107:intron 20.0%
!! TAAGTTGTTTATATCTAAAC+AGG + chr5.4:22245729-22245748 None:intergenic 20.0%
!! TAGATGATAATAAAAATGCA+GGG - chr5.4:22247656-22247675 MS.gene069107:CDS 20.0%
!! TCATTTCATTGATGTATTAA+AGG - chr5.4:22246895-22246914 MS.gene069107:intron 20.0%
!! TGAATAATTGAATATGAACA+TGG + chr5.4:22248566-22248585 None:intergenic 20.0%
!! TTTAGAGAAATTTAGAAACT+GGG - chr5.4:22245185-22245204 MS.gene069107:intron 20.0%
!!! AAAGGTATAAAGGAATTAAT+TGG - chr5.4:22245373-22245392 MS.gene069107:intron 20.0%
!!! AATTTTTTTTCTAATTTGGG+TGG - chr5.4:22244963-22244982 MS.gene069107:CDS 20.0%
!!! ATCTATATTTGTGTGTTTTT+GGG - chr5.4:22245217-22245236 MS.gene069107:intron 20.0%
!!! ATTTTAAGGTGTATATTATG+GGG - chr5.4:22247348-22247367 MS.gene069107:intron 20.0%
!!! GATTTTAAGGTGTATATTAT+GGG - chr5.4:22247347-22247366 MS.gene069107:intron 20.0%
!!! GTAATGTTTTTTTTTTTCCT+TGG - chr5.4:22245025-22245044 MS.gene069107:intron 20.0%
!!! GTATTTTAAAGCCTTTTAAT+TGG - chr5.4:22246588-22246607 MS.gene069107:intron 20.0%
!!! GTTAAGATTAATTTTTGGAT+TGG - chr5.4:22245310-22245329 MS.gene069107:intron 20.0%
!!! TCCTGATTTGTTTTTTTTTT+TGG - chr5.4:22245108-22245127 MS.gene069107:intron 20.0%
!!! TCGTTATGATTTTAGTTTAT+TGG - chr5.4:22246089-22246108 MS.gene069107:intron 20.0%
!!! TCTTTAAACAACTTTCATTT+TGG - chr5.4:22246239-22246258 MS.gene069107:intron 20.0%
!!! TGATTTTAAGGTGTATATTA+TGG - chr5.4:22247346-22247365 MS.gene069107:intron 20.0%
!!! TTAGCGTTAAGATTAATTTT+TGG - chr5.4:22245305-22245324 MS.gene069107:intron 20.0%
!!! TTGTTTTTTTTTTTGGTAGT+TGG - chr5.4:22245115-22245134 MS.gene069107:intron 20.0%
!!! TTTTAGAGAAATTTAGAAAC+TGG - chr5.4:22245184-22245203 MS.gene069107:intron 20.0%
! AACACACATAAAATAATCCA+AGG + chr5.4:22245045-22245064 None:intergenic 25.0%
! AATTATAGATCACATGGTTT+AGG - chr5.4:22245441-22245460 MS.gene069107:intron 25.0%
! ACAACTTAATTAAACGCTTA+TGG - chr5.4:22245741-22245760 MS.gene069107:CDS 25.0%
! CCATGTGATCTATAATTAAT+AGG + chr5.4:22245438-22245457 None:intergenic 25.0%
! CCTATTAATTATAGATCACA+TGG - chr5.4:22245435-22245454 MS.gene069107:intron 25.0%
! GTAGATGATAATAAAAATGC+AGG - chr5.4:22247655-22247674 MS.gene069107:CDS 25.0%
! TAAATACCTTATCAAATCGA+AGG + chr5.4:22247699-22247718 None:intergenic 25.0%
! TAATAGATATTGAGCATGTT+TGG + chr5.4:22246000-22246019 None:intergenic 25.0%
! TAATGTTAAAGTTATTGGTC+CGG + chr5.4:22247134-22247153 None:intergenic 25.0%
! TATCATAAAATTGTGTCCAA+TGG - chr5.4:22245614-22245633 MS.gene069107:CDS 25.0%
! TATTCATTATGAGATGCATT+TGG - chr5.4:22248580-22248599 MS.gene069107:intron 25.0%
! TCATAGAAATTACTAGTACA+CGG - chr5.4:22248788-22248807 MS.gene069107:intron 25.0%
! TCGACTAATGTTAAAGTTAT+TGG + chr5.4:22247139-22247158 None:intergenic 25.0%
! TCTAGACAACATATTTATGA+TGG - chr5.4:22248910-22248929 MS.gene069107:intron 25.0%
! TTAGAGAAATTTAGAAACTG+GGG - chr5.4:22245186-22245205 MS.gene069107:intron 25.0%
!! AGTTACTTGCAATTAATTTG+AGG - chr5.4:22245512-22245531 MS.gene069107:intron 25.0%
!! GATCTATATTTGTGTGTTTT+TGG - chr5.4:22245216-22245235 MS.gene069107:intron 25.0%
!! GTGTAATAATATTTTATGGC+AGG - chr5.4:22247896-22247915 MS.gene069107:intron 25.0%
!! TAATATTTTATGGCAGGATA+TGG - chr5.4:22247902-22247921 MS.gene069107:intron 25.0%
!! TATGTGTATATGGATTGTTT+AGG + chr5.4:22249159-22249178 None:intergenic 25.0%
!! TGCAATTAATTTGAGGAAAA+GGG - chr5.4:22245519-22245538 MS.gene069107:intron 25.0%
!! TTATCTAATCATTTTGTCAC+TGG - chr5.4:22246473-22246492 MS.gene069107:CDS 25.0%
!! TTGCAATTAATTTGAGGAAA+AGG - chr5.4:22245518-22245537 MS.gene069107:intron 25.0%
!!! TAAAGATATGGCTGATTTTA+AGG - chr5.4:22247334-22247353 MS.gene069107:intron 25.0%
!!! TATTGTATTATTTTGTTCCG+TGG - chr5.4:22246795-22246814 MS.gene069107:intron 25.0%
!!! TCTATATTTGTGTGTTTTTG+GGG - chr5.4:22245218-22245237 MS.gene069107:intron 25.0%
AAAATCATAACGAGATGCTA+AGG + chr5.4:22246082-22246101 None:intergenic 30.0%
AAAGCAAGTTCCAAGTTATA+AGG + chr5.4:22246124-22246143 None:intergenic 30.0%
AACAGTCTCATAAGTACTTA+TGG - chr5.4:22245948-22245967 MS.gene069107:CDS 30.0%
AAGAGACATGATGAATGAAA+AGG + chr5.4:22244878-22244897 None:intergenic 30.0%
AAGTACTTATGAGACTGTTT+GGG + chr5.4:22245947-22245966 None:intergenic 30.0%
AAGTTCCAAGTTATAAGGTA+TGG + chr5.4:22246119-22246138 None:intergenic 30.0%
ACAGGATTTAATTCAAGGTA+TGG + chr5.4:22246733-22246752 None:intergenic 30.0%
ACATGATTAAACCTTATGAG+AGG + chr5.4:22248138-22248157 None:intergenic 30.0%
ACTTGAACTTGAAGACTATA+AGG - chr5.4:22249016-22249035 MS.gene069107:intron 30.0%
AGACAAATGCATGTAACAAT+AGG + chr5.4:22247859-22247878 None:intergenic 30.0%
ATAAAATTGTGTCCAATGGA+AGG - chr5.4:22245618-22245637 MS.gene069107:CDS 30.0%
ATACTCTAAATATGATACGG+AGG + chr5.4:22248672-22248691 None:intergenic 30.0%
ATATTTATGATGGTACTGCA+AGG - chr5.4:22248920-22248939 MS.gene069107:intron 30.0%
ATCAATATTAAGCCTTCCAT+TGG + chr5.4:22245633-22245652 None:intergenic 30.0%
ATGACACTTAATACATGAGA+TGG - chr5.4:22248850-22248869 MS.gene069107:intron 30.0%
ATGATGAATGAAAAGGAACA+AGG + chr5.4:22244871-22244890 None:intergenic 30.0%
ATTCATGACTTGAAATGTTC+CGG - chr5.4:22247112-22247131 MS.gene069107:CDS 30.0%
ATTCGAGGATATATTTGATG+TGG - chr5.4:22246871-22246890 MS.gene069107:intron 30.0%
CAGGATTTAATTCAAGGTAT+GGG + chr5.4:22246732-22246751 None:intergenic 30.0%
CTCTTCAACTTCTATGTATT+CGG - chr5.4:22244893-22244912 MS.gene069107:CDS 30.0%
GACAAATGCATGTAACAATA+GGG + chr5.4:22247858-22247877 None:intergenic 30.0%
GGTGGTGAAAGAAATTATAT+GGG - chr5.4:22245242-22245261 MS.gene069107:intron 30.0%
GTGGTGAAAGAAATTATATG+GGG - chr5.4:22245243-22245262 MS.gene069107:intron 30.0%
TAAAGTAACTTTCACTCACT+TGG + chr5.4:22246410-22246429 None:intergenic 30.0%
TAAGTACTTATGAGACTGTT+TGG + chr5.4:22245948-22245967 None:intergenic 30.0%
TACTCTAAATATGATACGGA+GGG + chr5.4:22248671-22248690 None:intergenic 30.0%
TATGCATGGAGTCATATATA+TGG + chr5.4:22248477-22248496 None:intergenic 30.0%
TCAAGATAGCTTATGAAAAC+AGG + chr5.4:22245856-22245875 None:intergenic 30.0%
TTTATATCTTGCCTCTCATA+AGG - chr5.4:22248124-22248143 MS.gene069107:intron 30.0%
! GCATTTAAAAGTACAGATTC+AGG + chr5.4:22247086-22247105 None:intergenic 30.0%
! GGAATTATGTGACTTTTACA+AGG + chr5.4:22245708-22245727 None:intergenic 30.0%
! TGGTGGTGAAAGAAATTATA+TGG - chr5.4:22245241-22245260 MS.gene069107:intron 30.0%
!! ATGAATGCTTTGTTGATGAT+AGG - chr5.4:22248978-22248997 MS.gene069107:intron 30.0%
!! GTTTTTGTTTGATGCAGTTA+TGG - chr5.4:22247424-22247443 MS.gene069107:intron 30.0%
AAAATTGAAGGGGCAGAATT+AGG - chr5.4:22245576-22245595 MS.gene069107:CDS 35.0%
AAACAGAATTCCTGTGAACA+TGG - chr5.4:22248609-22248628 MS.gene069107:intron 35.0%
AACAGAATTCCTGTGAACAT+GGG - chr5.4:22248610-22248629 MS.gene069107:intron 35.0%
AATGCATTGTGCATATTTCC+TGG + chr5.4:22248432-22248451 None:intergenic 35.0%
ACACTTAATACATGAGATGG+AGG - chr5.4:22248853-22248872 MS.gene069107:intron 35.0%
ACCTTGAATTAAATCCTGTG+TGG - chr5.4:22246734-22246753 MS.gene069107:intron 35.0%
ACTAATGTAGTAGTCAAGTG+CGG + chr5.4:22248379-22248398 None:intergenic 35.0%
ACTGTTCTTGAACAAAACCA+TGG - chr5.4:22249062-22249081 MS.gene069107:intron 35.0%
AGGAAATATGCACAATGCAT+TGG - chr5.4:22248431-22248450 MS.gene069107:intron 35.0%
AGGTAACTACTAACCTTTGT+AGG + chr5.4:22247066-22247085 None:intergenic 35.0%
ATATTGGCAATGCATATGCA+TGG + chr5.4:22248491-22248510 None:intergenic 35.0%
ATTTCCGAAAGCTGTGATAT+TGG + chr5.4:22246332-22246351 None:intergenic 35.0%
CAAAGTATAGACAGGTGATT+TGG - chr5.4:22247979-22247998 MS.gene069107:intron 35.0%
CAACTCTTCATCAGTGAATT+GGG + chr5.4:22248024-22248043 None:intergenic 35.0%
CACGAAAGAAAGGAAAAACT+AGG + chr5.4:22246059-22246078 None:intergenic 35.0%
CACTTAATACATGAGATGGA+GGG - chr5.4:22248854-22248873 MS.gene069107:intron 35.0%
GAAATTTAGAAACTGGGGTT+TGG - chr5.4:22245191-22245210 MS.gene069107:intron 35.0%
GATATTGAGCATGTTTGGAT+TGG + chr5.4:22245995-22246014 None:intergenic 35.0%
GCTTTCCATACCTTATAACT+TGG - chr5.4:22246111-22246130 MS.gene069107:intron 35.0%
GGCAGAATTAGGAATTGTTT+AGG - chr5.4:22245587-22245606 MS.gene069107:CDS 35.0%
TACATCCAGGTATTTCTTGA+TGG - chr5.4:22246536-22246555 MS.gene069107:CDS 35.0%
TAGGCAAGTTATCAAAACTC+AGG + chr5.4:22247480-22247499 None:intergenic 35.0%
TATCACAGCTTTCGGAAATA+TGG - chr5.4:22246333-22246352 MS.gene069107:CDS 35.0%
TATTCGAGATACCAGAATCA+AGG - chr5.4:22246988-22247007 MS.gene069107:CDS 35.0%
TCAACTCTTCATCAGTGAAT+TGG + chr5.4:22248025-22248044 None:intergenic 35.0%
TCACAGGAATTCTGTTTGTA+TGG + chr5.4:22248606-22248625 None:intergenic 35.0%
TCCACACAGGATTTAATTCA+AGG + chr5.4:22246738-22246757 None:intergenic 35.0%
TGAATTAAATCCTGTGTGGA+AGG - chr5.4:22246738-22246757 MS.gene069107:intron 35.0%
TTGCTTCAAAGTAGAAATCC+TGG + chr5.4:22248274-22248293 None:intergenic 35.0%
TTGGAAATCTTGCGATGAAT+CGG - chr5.4:22246376-22246395 MS.gene069107:CDS 35.0%
! AACATTTTCCAGATACCAGT+TGG + chr5.4:22247035-22247054 None:intergenic 35.0%
! ACGATGTAATGTATTGATCG+AGG - chr5.4:22246825-22246844 MS.gene069107:intron 35.0%
! ACTTGGAACTTGCTTTATGT+AGG - chr5.4:22246128-22246147 MS.gene069107:intron 35.0%
! CTTTGAGAACACATGATGTT+TGG - chr5.4:22246436-22246455 MS.gene069107:CDS 35.0%
! GCTAGGCTAGTTACTAATAT+TGG + chr5.4:22248507-22248526 None:intergenic 35.0%
! GGACATGCTATTACTCATTT+TGG - chr5.4:22245462-22245481 MS.gene069107:intron 35.0%
! TAAAACATCTTCCCTTTTCC+AGG - chr5.4:22248233-22248252 MS.gene069107:intron 35.0%
! TGAGTGAGAGAAAGGTATAA+AGG - chr5.4:22245363-22245382 MS.gene069107:intron 35.0%
! TTCCAACCTTGATTTTCTAG+TGG + chr5.4:22246362-22246381 None:intergenic 35.0%
!! ATGCTTTGTTGATGATAGGT+TGG - chr5.4:22248982-22249001 MS.gene069107:intron 35.0%
!! GAGTTTTGATAACTTGCCTA+TGG - chr5.4:22247480-22247499 MS.gene069107:CDS 35.0%
!! TATTACTCATTTTGGTGTGG+TGG - chr5.4:22245470-22245489 MS.gene069107:intron 35.0%
!! TGCTATTACTCATTTTGGTG+TGG - chr5.4:22245467-22245486 MS.gene069107:intron 35.0%
!! TTTTGGATTGGGAATTTGAC+AGG - chr5.4:22245322-22245341 MS.gene069107:intron 35.0%
!!! ATATTTGTGTGTTTTTGGGG+TGG - chr5.4:22245221-22245240 MS.gene069107:intron 35.0%
AAAGTAGACAAGGTCAACTC+AGG - chr5.4:22249124-22249143 MS.gene069107:CDS 40.0%
AATCTCCAAGTGCTCTGATA+TGG + chr5.4:22247558-22247577 None:intergenic 40.0%
AATGCATGTAACAATAGGGC+AGG + chr5.4:22247854-22247873 None:intergenic 40.0%
ACCTTGACTTAACCATGTTG+AGG - chr5.4:22248540-22248559 MS.gene069107:intron 40.0%
ACTACTAACCTTTGTAGGAC+AGG + chr5.4:22247061-22247080 None:intergenic 40.0%
AGGAACAGGGTATGTGTATA+TGG + chr5.4:22249169-22249188 None:intergenic 40.0%
AGGTCCATTATCAGTATGCT+AGG + chr5.4:22248524-22248543 None:intergenic 40.0%
ATATGAACATGGCCTCAACA+TGG + chr5.4:22248555-22248574 None:intergenic 40.0%
ATCCAGGTATTTCTTGATGG+AGG - chr5.4:22246539-22246558 MS.gene069107:CDS 40.0%
ATCTCCAAGTGCTCTGATAT+GGG + chr5.4:22247557-22247576 None:intergenic 40.0%
ATCTTCATTTCTGCTTCACC+AGG - chr5.4:22248411-22248430 MS.gene069107:intron 40.0%
ATGCATGTAACAATAGGGCA+GGG + chr5.4:22247853-22247872 None:intergenic 40.0%
ATGTATCCTTCAGACTTCTC+TGG + chr5.4:22246521-22246540 None:intergenic 40.0%
ATTACATCGTGCACAATCCA+CGG + chr5.4:22246815-22246834 None:intergenic 40.0%
CACCACTAGAAAATCAAGGT+TGG - chr5.4:22246357-22246376 MS.gene069107:CDS 40.0%
CTACATTTGTGACCTCTTGA+AGG + chr5.4:22247639-22247658 None:intergenic 40.0%
CTAGCCTAGCATACTGATAA+TGG - chr5.4:22248517-22248536 MS.gene069107:intron 40.0%
CTTGCGATGAATCGGAAAAT+AGG - chr5.4:22246384-22246403 MS.gene069107:CDS 40.0%
GAAAGTAGTGAGTGAGAGAA+AGG - chr5.4:22245355-22245374 MS.gene069107:intron 40.0%
GAAGACTATAAGGCCAAACA+TGG - chr5.4:22249026-22249045 MS.gene069107:intron 40.0%
GAAGCAATTGTTTCCTCTGA+TGG - chr5.4:22248287-22248306 MS.gene069107:intron 40.0%
GAAGCCAATATCACAGCTTT+CGG - chr5.4:22246325-22246344 MS.gene069107:CDS 40.0%
GAAGGTCATGAAAGTAGACA+AGG - chr5.4:22249114-22249133 MS.gene069107:CDS 40.0%
GATGAAGAGTTGAGAAGTCA+GGG - chr5.4:22248032-22248051 MS.gene069107:intron 40.0%
GCAAGTTATCAAAACTCAGG+CGG + chr5.4:22247477-22247496 None:intergenic 40.0%
TCTTGAACAAAACCATGGAG+TGG - chr5.4:22249067-22249086 MS.gene069107:intron 40.0%
TGATGAAGAGTTGAGAAGTC+AGG - chr5.4:22248031-22248050 MS.gene069107:intron 40.0%
TGGACACCACTAGAAAATCA+AGG - chr5.4:22246353-22246372 MS.gene069107:CDS 40.0%
TGGGGGAAAAGCTGAAAAAT+TGG - chr5.4:22247950-22247969 MS.gene069107:intron 40.0%
TTCAAAACAATGTTCAGCGC+AGG + chr5.4:22247264-22247283 None:intergenic 40.0%
TTTATCAGAGCACGCTCAAA+AGG + chr5.4:22248336-22248355 None:intergenic 40.0%
TTTGAGCGTGCTCTGATAAA+GGG - chr5.4:22248336-22248355 MS.gene069107:intron 40.0%
! ATGTTTTGCCTGTCCTACAA+AGG - chr5.4:22247050-22247069 MS.gene069107:CDS 40.0%
! CTCTTTTTGTCTTCCATCAG+AGG + chr5.4:22248303-22248322 None:intergenic 40.0%
! GAAAGTTGACTTTACAACGC+AGG + chr5.4:22247516-22247535 None:intergenic 40.0%
! TATAGACAGGTGATTTGGCA+GGG - chr5.4:22247984-22248003 MS.gene069107:intron 40.0%
! TCTGCACCTTCGATTTGATA+AGG - chr5.4:22247690-22247709 MS.gene069107:intron 40.0%
! TTTTGAGCGTGCTCTGATAA+AGG - chr5.4:22248335-22248354 MS.gene069107:intron 40.0%
!! CACTAGGGAGTACTATTCAT+TGG - chr5.4:22246964-22246983 MS.gene069107:CDS 40.0%
!! CGTGAGTGTCTAGGTTTTAT+AGG + chr5.4:22246162-22246181 None:intergenic 40.0%
AAACTCAGGCGGTGAGAAAA+TGG + chr5.4:22247466-22247485 None:intergenic 45.0%
ACCGAAATCACAGGCTGAAT+GGG + chr5.4:22247931-22247950 None:intergenic 45.0%
ACTTACTGTTTCACGCTGCA+CGG + chr5.4:22246199-22246218 None:intergenic 45.0%
ATCCTGTGTGGAAGGATTCA+AGG - chr5.4:22246746-22246765 MS.gene069107:intron 45.0%
ATTCAGCCTGTGATTTCGGT+GGG - chr5.4:22247931-22247950 MS.gene069107:intron 45.0%
CACGCTCAAAAGGCAAAGTA+AGG + chr5.4:22248326-22248345 None:intergenic 45.0%
CATGAATGTCCCATGTTCAC+AGG + chr5.4:22248622-22248641 None:intergenic 45.0%
CATTTCTTCAGACGAGCGTT+GGG + chr5.4:22247605-22247624 None:intergenic 45.0%
GAACTCCAACTACCTTCAAG+AGG - chr5.4:22247624-22247643 MS.gene069107:CDS 45.0%
GAAGTCTGAAGGATACATCC+AGG - chr5.4:22246523-22246542 MS.gene069107:CDS 45.0%
GACCTCCATCAAGAAATACC+TGG + chr5.4:22246544-22246563 None:intergenic 45.0%
GAGCTTAGCTTCCTTAACAG+AGG - chr5.4:22244819-22244838 MS.gene069107:CDS 45.0%
GATGCTAAGGCACGAAAGAA+AGG + chr5.4:22246069-22246088 None:intergenic 45.0%
GCATTACCAGAGAAGTCTGA+AGG - chr5.4:22246512-22246531 MS.gene069107:CDS 45.0%
GCCTCAACATGGTTAAGTCA+AGG + chr5.4:22248544-22248563 None:intergenic 45.0%
GGCTCTTGCAAAGTATAGAC+AGG - chr5.4:22247971-22247990 MS.gene069107:intron 45.0%
TAACCTTGGCACATGCAATC+AGG + chr5.4:22249189-22249208 None:intergenic 45.0%
TACCTTGAATCCTTCCACAC+AGG + chr5.4:22246751-22246770 None:intergenic 45.0%
TAGCAACAATCCGACTTCCT+CGG + chr5.4:22248075-22248094 None:intergenic 45.0%
TCATTTCTTCAGACGAGCGT+TGG + chr5.4:22247606-22247625 None:intergenic 45.0%
TCCACCATAGACCTGGAAAA+GGG + chr5.4:22248247-22248266 None:intergenic 45.0%
TCTCCAAGTGCTCTGATATG+GGG + chr5.4:22247556-22247575 None:intergenic 45.0%
TGACCCATCAATGCCATGTT+TGG + chr5.4:22249042-22249061 None:intergenic 45.0%
TGACTTTACAACGCAGGTGT+TGG + chr5.4:22247510-22247529 None:intergenic 45.0%
TGGTGGTTGAGGTTCTTTGT+TGG - chr5.4:22245487-22245506 MS.gene069107:intron 45.0%
TGTGACCTCTTGAAGGTAGT+TGG + chr5.4:22247632-22247651 None:intergenic 45.0%
TTTGAGCATGCAGTGGTTGA+AGG - chr5.4:22249096-22249115 MS.gene069107:intron 45.0%
! AAGGCCAAACATGGCATTGA+TGG - chr5.4:22249035-22249054 MS.gene069107:intron 45.0%
! ATTTCTTCAGACGAGCGTTG+GGG + chr5.4:22247604-22247623 None:intergenic 45.0%
! CAAGTGTTGCTCCTCTGTTA+AGG + chr5.4:22244833-22244852 None:intergenic 45.0%
! GGATTGTTGCTAGCTGCTTT+GGG - chr5.4:22248083-22248102 MS.gene069107:intron 45.0%
! GTATAGACAGGTGATTTGGC+AGG - chr5.4:22247983-22248002 MS.gene069107:intron 45.0%
! TCTTCCCTTTTCCAGGTCTA+TGG - chr5.4:22248240-22248259 MS.gene069107:intron 45.0%
!! AGGCCAAACATGGCATTGAT+GGG - chr5.4:22249036-22249055 MS.gene069107:intron 45.0%
!! GCTGTGATATTGGCTTCACA+AGG + chr5.4:22246322-22246341 None:intergenic 45.0%
!! GTTGATGATAGGTTGGACAC+AGG - chr5.4:22248989-22249008 MS.gene069107:intron 45.0%
!! TCATTTTGGTGTGGTGGTTG+AGG - chr5.4:22245476-22245495 MS.gene069107:intron 45.0%
!!! TTTGTGTGTTTTTGGGGTGG+TGG - chr5.4:22245224-22245243 MS.gene069107:intron 45.0%
!! AAATAATAATAATGTTATAT+TGG + chr5.4:22248728-22248747 None:intergenic 5.0%
!!! TTTAATTTTTTTTCTAATTT+GGG - chr5.4:22244960-22244979 MS.gene069107:CDS 5.0%
!!! TTTTAATTTTTTTTCTAATT+TGG - chr5.4:22244959-22244978 MS.gene069107:CDS 5.0%
AGCAACAATCCGACTTCCTC+GGG + chr5.4:22248074-22248093 None:intergenic 50.0%
AGCTTCCTGAAGTGTACTCG+TGG - chr5.4:22246939-22246958 MS.gene069107:intron 50.0%
AGGTCTGAACCAGCAAAGGA+TGG - chr5.4:22246559-22246578 MS.gene069107:CDS 50.0%
ATGGAGGTCTGAACCAGCAA+AGG - chr5.4:22246555-22246574 MS.gene069107:CDS 50.0%
CACCGAAATCACAGGCTGAA+TGG + chr5.4:22247932-22247951 None:intergenic 50.0%
CAGTACATGCAACAGCCAAC+TGG - chr5.4:22247017-22247036 MS.gene069107:CDS 50.0%
CAGTTGGCTGTTGCATGTAC+TGG + chr5.4:22247019-22247038 None:intergenic 50.0%
CATTCAGCCTGTGATTTCGG+TGG - chr5.4:22247930-22247949 MS.gene069107:intron 50.0%
CGGATTGTTGCTAGCTGCTT+TGG - chr5.4:22248082-22248101 MS.gene069107:intron 50.0%
CTCAAACTCTGACCACTCCA+TGG + chr5.4:22249082-22249101 None:intergenic 50.0%
CTCCACCATAGACCTGGAAA+AGG + chr5.4:22248248-22248267 None:intergenic 50.0%
GCCCATTCAGCCTGTGATTT+CGG - chr5.4:22247927-22247946 MS.gene069107:intron 50.0%
GGCACATGCAATCAGGAACA+GGG + chr5.4:22249182-22249201 None:intergenic 50.0%
GGTCTGAACCAGCAAAGGAT+GGG - chr5.4:22246560-22246579 MS.gene069107:CDS 50.0%
GTCAGAGTTTGAGCATGCAG+TGG - chr5.4:22249089-22249108 MS.gene069107:intron 50.0%
GTCTGAACCAGCAAAGGATG+GGG - chr5.4:22246561-22246580 MS.gene069107:CDS 50.0%
GTTCCTGATTGCATGTGCCA+AGG - chr5.4:22249183-22249202 MS.gene069107:CDS 50.0%
TACTGGTGCAGCCTTGATTC+TGG + chr5.4:22247002-22247021 None:intergenic 50.0%
TCTGAACCAGCAAAGGATGG+GGG - chr5.4:22246562-22246581 MS.gene069107:CDS 50.0%
TGCAACAGCCAACTGGTATC+TGG - chr5.4:22247024-22247043 MS.gene069107:CDS 50.0%
TGGCACATGCAATCAGGAAC+AGG + chr5.4:22249183-22249202 None:intergenic 50.0%
TTCAGCCTGTGATTTCGGTG+GGG - chr5.4:22247932-22247951 MS.gene069107:intron 50.0%
! AAGTGTACTCGTGGAGCACT+AGG - chr5.4:22246948-22246967 MS.gene069107:intron 50.0%
! ACAGGTGATTTGGCAGGGAA+GGG - chr5.4:22247989-22248008 MS.gene069107:intron 50.0%
! AGTGTACTCGTGGAGCACTA+GGG - chr5.4:22246949-22246968 MS.gene069107:intron 50.0%
! GTTCCCCATATCAGAGCACT+TGG - chr5.4:22247550-22247569 MS.gene069107:intron 50.0%
! TCCCTTTTCCAGGTCTATGG+TGG - chr5.4:22248243-22248262 MS.gene069107:intron 50.0%
! TTTTCCCCCACCGAAATCAC+AGG + chr5.4:22247940-22247959 None:intergenic 50.0%
!! CGCAGGTGTTGGATATCCAT+AGG + chr5.4:22247499-22247518 None:intergenic 50.0%
ATTGCGAAGGCGGCTGATGA+GGG + chr5.4:22247581-22247600 None:intergenic 55.0%
CCAATGACTCCCGAGGAAGT+CGG - chr5.4:22248062-22248081 MS.gene069107:intron 55.0%
CGACTTCCTCGGGAGTCATT+GGG + chr5.4:22248064-22248083 None:intergenic 55.0%
CTAGACACTCACGCCTTCTG+AGG - chr5.4:22246169-22246188 MS.gene069107:intron 55.0%
GTGCTCCACGAGTACACTTC+AGG + chr5.4:22246947-22246966 None:intergenic 55.0%
GTTTCTCTGCAGCGCATTCG+AGG - chr5.4:22246856-22246875 MS.gene069107:intron 55.0%
TACGTACCCCCATCCTTTGC+TGG + chr5.4:22246571-22246590 None:intergenic 55.0%
TCAGCCTGTGATTTCGGTGG+GGG - chr5.4:22247933-22247952 MS.gene069107:intron 55.0%
! CTCAGAAGGCGTGAGTGTCT+AGG + chr5.4:22246171-22246190 None:intergenic 55.0%
! GACAGGTGATTTGGCAGGGA+AGG - chr5.4:22247988-22248007 MS.gene069107:intron 55.0%
ACGTTGCCCAATGACTCCCG+AGG - chr5.4:22248055-22248074 MS.gene069107:intron 60.0%
CCGACTTCCTCGGGAGTCAT+TGG + chr5.4:22248065-22248084 None:intergenic 60.0%
GATTGCGAAGGCGGCTGATG+AGG + chr5.4:22247582-22247601 None:intergenic 60.0%
TGGCTGCTCCACCATAGACC+TGG + chr5.4:22248254-22248273 None:intergenic 60.0%
! AGCGTTGGGGATTGCGAAGG+CGG + chr5.4:22247591-22247610 None:intergenic 60.0%
! AGGTCTATGGTGGAGCAGCC+AGG - chr5.4:22248253-22248272 MS.gene069107:intron 60.0%
!! ACGAGCGTTGGGGATTGCGA+AGG + chr5.4:22247594-22247613 None:intergenic 60.0%
GCTGCACGGCGCTCCTCAGA+AGG + chr5.4:22246185-22246204 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 22244816 22249209 22244816 ID=MS.gene069107
chr5.4 mRNA 22244816 22249209 22244816 ID=MS.gene069107.t1;Parent=MS.gene069107
chr5.4 exon 22249111 22249209 22249111 ID=MS.gene069107.t1.exon1;Parent=MS.gene069107.t1
chr5.4 CDS 22249111 22249209 22249111 ID=cds.MS.gene069107.t1;Parent=MS.gene069107.t1
chr5.4 exon 22247813 22247875 22247813 ID=MS.gene069107.t1.exon2;Parent=MS.gene069107.t1
chr5.4 CDS 22247813 22247875 22247813 ID=cds.MS.gene069107.t1;Parent=MS.gene069107.t1
chr5.4 exon 22247615 22247696 22247615 ID=MS.gene069107.t1.exon3;Parent=MS.gene069107.t1
chr5.4 CDS 22247615 22247696 22247615 ID=cds.MS.gene069107.t1;Parent=MS.gene069107.t1
chr5.4 exon 22247442 22247512 22247442 ID=MS.gene069107.t1.exon4;Parent=MS.gene069107.t1
chr5.4 CDS 22247442 22247512 22247442 ID=cds.MS.gene069107.t1;Parent=MS.gene069107.t1
chr5.4 exon 22247258 22247346 22247258 ID=MS.gene069107.t1.exon5;Parent=MS.gene069107.t1
chr5.4 CDS 22247258 22247346 22247258 ID=cds.MS.gene069107.t1;Parent=MS.gene069107.t1
chr5.4 exon 22246954 22247157 22246954 ID=MS.gene069107.t1.exon6;Parent=MS.gene069107.t1
chr5.4 CDS 22246954 22247157 22246954 ID=cds.MS.gene069107.t1;Parent=MS.gene069107.t1
chr5.4 exon 22246314 22246584 22246314 ID=MS.gene069107.t1.exon7;Parent=MS.gene069107.t1
chr5.4 CDS 22246314 22246584 22246314 ID=cds.MS.gene069107.t1;Parent=MS.gene069107.t1
chr5.4 exon 22245880 22246107 22245880 ID=MS.gene069107.t1.exon8;Parent=MS.gene069107.t1
chr5.4 CDS 22245880 22246107 22245880 ID=cds.MS.gene069107.t1;Parent=MS.gene069107.t1
chr5.4 exon 22245573 22245770 22245573 ID=MS.gene069107.t1.exon9;Parent=MS.gene069107.t1
chr5.4 CDS 22245573 22245770 22245573 ID=cds.MS.gene069107.t1;Parent=MS.gene069107.t1
chr5.4 exon 22244816 22245025 22244816 ID=MS.gene069107.t1.exon10;Parent=MS.gene069107.t1
chr5.4 CDS 22244816 22245025 22244816 ID=cds.MS.gene069107.t1;Parent=MS.gene069107.t1
Gene Sequence

>MS.gene069107

ATGGAGCTTAGCTTCCTTAACAGAGGAGCAACACTTGTTGTTGTTTCTCTTTCCTTGTTCCTTTTCATTCATCATGTCTCTTCAACTTCTATGTATTCGGTGAGTTTAATAAATCTTCATTTTTTTTTTCTCGGAAGTTTTATTTTTAATTTTTTTTCTAATTTGGGTGGAACAGTAACATTACCGTTATGTCTTTTATTTCATTTTCAGTAATGTTTTTTTTTTTCCTTGGATTATTTTATGTGTGTTTGTTTAATTAATTTTTTGAGTAATTTACTTTCTTAAGTGTTTTTCCTGATTTGTTTTTTTTTTTGGTAGTTGGTTTTATTTTATTATCAGAAATCTTTTGAGTTTCATAGTAAATTTTATTTTAGAGAAATTTAGAAACTGGGGTTTGGTTGATCTATATTTGTGTGTTTTTGGGGTGGTGGTGAAAGAAATTATATGGGGTTTAGTTAATAAGCGTTTAGATTATTTGTTTGATGCTGTTTAGCGTTAAGATTAATTTTTGGATTGGGAATTTGACAGGCTTAGAAAATGAAAGTAGTGAGTGAGAGAAAGGTATAAAGGAATTAATTGGGTTGACTGAGTTAGTAAATTTGTGTAACTAATTTAACAGCCTATTAATTATAGATCACATGGTTTAGGACATGCTATTACTCATTTTGGTGTGGTGGTTGAGGTTCTTTGTTGGATAGTTACTTGCAATTAATTTGAGGAAAAGGGAATTAACAAAAAAAGGGAATTAGGAAAATAAAATAAAATTGAAGGGGCAGAATTAGGAATTGTTTAGGTAAATATCATAAAATTGTGTCCAATGGAAGGCTTAATATTGATAATGAAAAAAAAAAAATTGTCAAAAAAAATGATATGATGCTTGGTTAACTTGCCTTGTAAAAGTCACATAATTCCTGTTTAGATATAAACAACTTAATTAAACGCTTATGGCATAAGCACTTATCATATAGTGCTTATATGTAAGCTATTTCTGTAGCAAATGATAAATTAAAGTCAAATTGTTCTTGTATAAGCTATAACCTGTTTTCATAAGCTATCTTGAAATTTATATGATTGGAAATAAGCTGAAAACAACTTATGCACATGTCATAAGCTATTTCTATAAGCTCTCCCAAACAGTCTCATAAGTACTTATGGTAGTTGATAAACTCAAATAGACCAATCCAAACATGCTCAATATCTATTAGAATGAATAACATCATCGTGTTCATGCTGTTTAATTCCTAGTTTTTCCTTTCTTTCGTGCCTTAGCATCTCGTTATGATTTTAGTTTATTGGCTTTCCATACCTTATAACTTGGAACTTGCTTTATGTAGGAAATGAATCCTATAAAACCTAGACACTCACGCCTTCTGAGGAGCGCCGTGCAGCGTGAAACAGTAAGTTAATTGAGCAGCGAATGCTTTCTTTAAACAACTTTCATTTTGGTTTTTGTTAGTTTTGATTGTTCATTTTATATATTTTTATAATTTGTTATTGTTTTCTCCTTGTGAAGCCAATATCACAGCTTTCGGAAATATGGACACCACTAGAAAATCAAGGTTGGAAATCTTGCGATGAATCGGAAAATAGGCCAAGTGAGTGAAAGTTACTTTATTTTATCTTTGAGAACACATGATGTTTGGTTATGTATATTTCTTTATCTAATCATTTTGTCACTGGAACTTGAATTGAAGTAGCATTACCAGAGAAGTCTGAAGGATACATCCAGGTATTTCTTGATGGAGGTCTGAACCAGCAAAGGATGGGGGTACGTATTTTAAAGCCTTTTAATTGGATCAATCTTTTTCTTTCGTGTATGCGAAAGATTTATAATTTTTACACGGTGTTTAATTATTTTTTTAGATATGTGATGCAGTTGCTGTTGCGAAGATACTGAATGCAACTCTTGTGATCCCATACCTTGAATTAAATCCTGTGTGGAAGGATTCAAGGTAGTGTCAGAAACTAGAACTCCAATTTATTGTATTATTTTGTTCCGTGGATTGTGCACGATGTAATGTATTGATCGAGGTTGCTTATGTTTCTCTGCAGCGCATTCGAGGATATATTTGATGTGGATCATTTCATTGATGTATTAAAGGATGATGTTTCTATAGTTAAAGAGCTTCCTGAAGTGTACTCGTGGAGCACTAGGGAGTACTATTCATTGGCTATTCGAGATACCAGAATCAAGGCTGCACCAGTACATGCAACAGCCAACTGGTATCTGGAAAATGTTTTGCCTGTCCTACAAAGGTTAGTAGTTACCTGAATCTGTACTTTTAAATGCTAGTGCATTCATGACTTGAAATGTTCCGGACCAATAACTTTAACATTAGTCGATTCAGTGACTAAGATTATAGACACTTTTATTCATAATTGCAACTGCAACTGCTTTAGCATTTATATAACGTTATCAATTATATAACGTTATCAATTACAGAACCTGCGCTGAACATTGTTTTGAAGATTATTTATTACTTCTTTGTTTTTAAATAATCTTTCTATCGATTTTAAATAAAGATATGGCTGATTTTAAGGTGTATATTATGGGGATGCTGCAAGCTCTGTATATTCTCTTTGAATTTTGAAATTCTAATATCATGGTGTTTTTGTTTGATGCAGTTATGGTGTTGCTGCAATCTCTCCATTTTCTCACCGCCTGAGTTTTGATAACTTGCCTATGGATATCCAACACCTGCGTTGTAAAGTCAACTTTCAAGCTTTAGACTTTGTTCCCCATATCAGAGCACTTGGAGATTCCCTCATCAGCCGCCTTCGCAATCCCCAACGCTCGTCTGAAGAAATGAACTCCAACTACCTTCAAGAGGTCACAAATGTAGATGATAATAAAAATGCAGGGAAATTTGTTGTTCTGCACCTTCGATTTGATAAGGTATTTATCTTTTTCTCATTTATCATCATCATTGTCATCATCTGTACACGCAATGTTGTAAAATTTGTCTCTGTTTCTTAATTTTTCTTGTTTGTTCGTATAACTATCTTGACGTTACAGTAGTGCTAGCAACACACTCCCTGCCCTATTGTTACATGCATTTGTCTGTAAATTGAAAAAGTTTGTGTAATAATATTTTATGGCAGGATATGGCAGCCCATTCAGCCTGTGATTTCGGTGGGGGAAAAGCTGAAAAATTGGCTCTTGCAAAGTATAGACAGGTGATTTGGCAGGGAAGGGTTCTTAATTCCCAATTCACTGATGAAGAGTTGAGAAGTCAGGGACGTTGCCCAATGACTCCCGAGGAAGTCGGATTGTTGCTAGCTGCTTTGGGATTCGACAACAGCACTCGTTTATATCTTGCCTCTCATAAGGTTTAATCATGTTATTTTTTAGTTTGAAACAGTAGAGTCTCATTCATTTTAACTATTAGTGCTTGTTTTAAAATGATTATGTTTTTCTAAAACATCTTCCCTTTTCCAGGTCTATGGTGGAGCAGCCAGGATTTCTACTTTGAAGCAATTGTTTCCTCTGATGGAAGACAAAAAGAGCCTTACTTTGCCTTTTGAGCGTGCTCTGATAAAGGGAAAAGCTTCGCTATTAGCCGCACTTGACTACTACATTAGTATACATAGTGACATCTTCATTTCTGCTTCACCAGGAAATATGCACAATGCATTGGTGAGTTCTCCCTAAAATTTACCATATATATGACTCCATGCATATGCATTGCCAATATTAGTAACTAGCCTAGCATACTGATAATGGACCTTGACTTAACCATGTTGAGGCCATGTTCATATTCAATTATTCATTATGAGATGCATTTGGCCATACAAACAGAATTCCTGTGAACATGGGACATTCATGAGTTTGTCATATAACAATTAATTACTCCCTCCGTATCATATTTAGAGTATTGTTTGTCTCACATTATTTGTCATTTTAAAATACCAATATAACATTATTATTATTTTTACTGATATACTCTTATCTAGTAAATGAAACACATTTTATCATAGAAATTACTAGTACACGGAATAATTTTCTTAAAAAGTGTGTATAACTATAAATGACAATGACACTTAATACATGAGATGGAGGGAGTAACACTATAAGAAATCTGTGTTTAGAACTGTCTAGACAACATATTTATGATGGTACTGCAAGGCTGTTGAAACAGTTGAATTGAATTTATAAATGATAATGAATGCTTTGTTGATGATAGGTTGGACACAGGACATACTTGAACTTGAAGACTATAAGGCCAAACATGGCATTGATGGGTCAACTGTTCTTGAACAAAACCATGGAGTGGTCAGAGTTTGAGCATGCAGTGGTTGAAGGTCATGAAAGTAGACAAGGTCAACTCAGGCTTAGAAAGCCTAAACAATCCATATACACATACCCTGTTCCTGATTGCATGTGCCAAGGTTAA
Protein sequence

>MS.gene069107.t1

MELSFLNRGATLVVVSLSLFLFIHHVSSTSMYSEMNPIKPRHSRLLRSAVQRETPISQLSEIWTPLENQGWKSCDESENRPTLPEKSEGYIQVFLDGGLNQQRMGICDAVAVAKILNATLVIPYLELNPVWKDSSAFEDIFDVDHFIDVLKDDVSIVKELPEVYSWSTREYYSLAIRDTRIKAAPVHATANWYLENVLPVLQSYGVAAISPFSHRLSFDNLPMDIQHLRCKVNFQALDFVPHIRALGDSLISRLRNPQRSSEEMNSNYLQEVTNVDDNKNAGKFVVLHLRFDKDMAAHSACDFGGGKAEKLALAKYRQVIWQGRVLNSQFTDEELRSQGRCPMTPEEVGLLLAALGFDNSTRLYLASHKVYGGAARISTLKQLFPLMEDKKSLTLPFERALIKGKASLLAALDYYISIHSDIFISASPGNMHNALVGHRTYLNLKTIRPNMALMGQLFLNKTMEWSEFEHAVVEGHESRQGQLRLRKPKQSIYTYPVPDCMCQG